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Three-dimensional human intestinal organoids (hIO) are widely used as a platform for biological and biomedical research. However, reproducibility and challenges for large-scale expansion limit their applicability. Here, we establish a human intestinal stem cell (ISC) culture method expanded under feeder-free and fully defined conditions through selective enrichment of ISC populations (ISC 3D-hIO ) within hIO derived from human pluripotent stem cells. The intrinsic self-organisation property of ISC 3D-hIO , combined with air-liquid interface culture in a minimally defined medium, forces ISC 3D-hIO to differentiate into the intestinal epithelium with cellular diversity, villus-like structure, and barrier integrity. Notably, ISC 3D-hIO is an ideal cell source for gene editing to study ISC biology and transplantation for intestinal diseases. We demonstrate the intestinal epithelium differentiated from ISC 3D-hIO as a model system to study severe acute respiratory syndrome coronavirus 2 viral infection. ISC 3D-hIO culture technology provides a biological tool for use in regenerative medicine and disease modelling., (© 2024. The Author(s).)

With rising concerns about the functional role of long-term care hospitals in the Korean medical system, this study aimed to observe the experience of admission in the long-term care hospitals and their association with medical expenditures among patients with colorectal cancer, and to investigate disparities among vulnerable populations. Data were obtained from the National Health Insurance Senior Cohort Database in South Korea for the period 2008-2019. With 6,305 patients newly diagnosed with colorectal cancer between 2008 and 2015, we conducted a regression analysis using the Generalized Estimating Equation model with gamma distribution to investigate the association between health expenditure and the experience of long-term care hospitals. We also explored the interaction effect of disability or income, followed by subgroup analysis. Among patients who received care at long-term care hospitals, the health expenditure within one year and five years after the incidence of colorectal cancer was found to be higher than in those who did not receive such care. It was observed that the low-income and disabled groups experienced higher disparities in health expenditure. The rise in health expenditure highlights importance for functional improvement, aligning with these initial purpose of long-term care hospitals to address the growing healthcare needs of the elderly population and ensure efficient healthcare spending, of long-term care hospitals. To achieve this original intent, it is imperative for government initiatives to focus on reducing quality gaps in long-term care hospital services and addressing cost disparities among individuals with cancer, including those with disabilities or low-income., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three Lactobacillus strains ( Limosilactobacillus reuteri KGC1901, Lacticaseibacillus casei KGC1201, Limosilactobacillus fermentum KGC1601) isolated from Panax ginseng , using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.

This study focuses on the electrochemical properties of layered double hydroxide (LDH), which is a specific structure of NiCoCu LDH, and the active species therein, rather than the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) of ternary NiCoCu LDH materials. Six types of catalysts were synthesized using the reflux condenser method and coated onto a nickel foam support electrode. Compared to bare, binary, and ternary electrocatalysts, the NiCoCu LDH electrocatalyst exhibited higher stability. The double layer capacitance (Cdl) of the NiCoCu LDH (12.3 mF cm -2 ) is greater than that of the bare and binary electrocatalysts, indicating that the NiCoCu LDH electrocatalyst has a larger electrochemical active surface area. In addition, the NiCoCu LDH electrocatalyst has a lower overpotential of 87 mV and 224 mV for the HER and OER, respectively, indicating its excellent activity with the bare and binary electrocatalysts. Finally, it is demonstrated that the structural characteristics of the NiCoCu LDH contribute to its excellent stability in long-term HER and OER tests., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: RAJA ANNAMALAI reports financial support was provided by Yeungnam University. RAJA ANNAMALAI reports a relationship with Yeungnam University that includes: employment. RAJA ANNAMALAI has patent pending to RAJA ANNAMALAI. None., (Copyright © 2023 Elsevier Inc. All rights reserved.)

Background: Child anxiety disorders are highly prevalent yet undertreated. As parents are often 'gatekeepers' to children receiving treatment and support, this study aimed to investigate modifiable parental factors affecting professional help-seeking for their children from general practitioners (GPs), psychologists, and paediatricians., Methods: In this study, 257 Australian parents of children aged 5-12 years with elevated anxiety symptoms completed a cross-sectional online survey. The survey assessed help-seeking from a GP, psychologist, and paediatrician (General Help Seeking Questionnaire), as well as anxiety knowledge (Anxiety Literacy Scale), help-seeking attitudes (Attitudes Toward Seeking Professional Psychological Help), personal stigma (Generalised Anxiety Stigma Scale) and self-efficacy (Self-Efficacy in Seeking Mental Health Care)., Results: 66.9% of participants had sought help from a GP, 61.1% from a psychologist, and 33.9% from a paediatrician. Help-seeking from a GP or psychologist was associated with lower personal stigma (p = .02 and p = .03 respectively). Participants who sought help from a psychologist also had more positive attitudes toward seeking professional support (p = .01). Conversely, knowledge of anxiety disorders and self-efficacy were not associated with help-seeking from any source., Limitations: Limitations of the study include the representativeness of our sample (female gender, higher education level); unexplained variance potentially accounted for by other factors (e.g., structural barriers); lack of prior validation of measures in a parent sample., Conclusions: This research will inform the development of public health policy and psychoeducation interventions for parents, to reduce personal stigma and increase positive attitudes towards professional help-seeking, in turn improving help-seeking for child anxiety., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

A hydrothermal technique was employed to synthesize a Sr 2 Nb 2 O 7 -rGO-ZnO (SNRZ) ternary nanocatalyst, in which ZnO and Sr 2 Nb 2 O 7 were deposited on reduced graphene oxide (rGO) sheets. The surface morphologies, optical properties, and chemical states, of the photocatalysts were characterized to understand their properties. The SNRZ ternary photocatalyst was superior over the reduction of Cr (VI) to harmless Cr (III) compared to the efficiencies obtained using bare, binary, and composite catalysts. The effects of various parameters, including the solution pH and weight ratio, on the photocatalytic reduction of Cr (VI) were investigated. The highest photocatalytic reduction performance (97.6%) was achieved at pH 4 and a reaction time of 70 min. Photoluminescence emission measurements were used to confirm efficient charge migration and separation across the SNRZ, which improved the reduction of Cr (VI). A feasible reduction mechanism for the SNRZ photocatalyst is proposed. This study presents an effective, inexpensive, non-toxic, and stable catalyst, for the reduction of Cr (VI) to Cr (III) using SNRZ ternary nanocatalysts., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Annamalai Raja reports financial support was provided by Yeungnam University. Annamalai Raja reports a relationship with Yeungnam University that includes: board membership. Annamalai Raja has patent nil pending to nil. None., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Background: A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing the GI epithelial cells to function less efficiently, leading to inflammation and an imbalance in the gut microbiome. PM10 may, however, act as an exacerbation factor in patients with inflamed intestinal epithelium, which is associated with inflammatory bowel disease., Objective: The purpose of this study was to dissect the pathology mechanism of PM10 exposure in inflamed intestines., Methods: In this study, we established chronically inflamed intestinal epithelium models utilizing two-dimensional (2D) human intestinal epithelial cells (hIECs) and 3D human intestinal organoids (hIOs), which mimic in vivo cellular diversity and function, in order to examine the deleterious effects of PM10 in human intestine-like in vitro models., Results: Inflamed 2D hIECs and 3D hIOs exhibited pathological features, such as inflammation, decreased intestinal markers, and defective epithelial barrier function. In addition, we found that PM10 exposure induced a more severe disturbance of peptide uptake in inflamed 2D hIECs and 3D hIOs than in control cells. This was due to the fact that it interferes with calcium signaling, protein digestion, and absorption pathways. The findings demonstrate that PM10-induced epithelial alterations contribute to the exacerbation of inflammatory disorders caused by the intestine., Conclusions: According to our findings, 2D hIEC and 3D hIO models could be powerful in vitro platforms for the evaluation of the causal relationship between PM exposure and abnormal human intestinal functions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Son, Son, Yu, Baek, Park, Lee, Lee, Kim and Son.)

Patient-derived human induced pluripotent stem cells (iPSCs) provide a potentially useful resource for studying disease pathology and therapeutics. In this study, we generated the breast cancer patient-derived KRIBBi009-A-iPSC line from normal fibroblasts using the Sendai virus, which expressed pluripotent markers and exhibited differentiation capacity across 3 germ layers through in vitro differentiation and in vivo teratoma assay. A normal karyotype and the absence of cross-contamination of the cell lines were confirmed. Consequently, the developed iPSC line has been confirmed to be suitable for use in various studies., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

Leakage accidents at chemical facilities have a negative impact on both the environment and human life, and the government has established and implemented regulations on dikes in order to minimize such accidents. However, the overtopping phenomenon in which chemicals overflow the dike due to catastrophic leakage requires additional safeguards. In this study, the mitigation effect was confirmed by simulating tanks and dikes using various deflector plates to minimize the effect of spilled chemicals. ANSYS Fluent 19.1, a computational fluid dynamics program, was used, and the overtopping effect was compared with a dike design that satisfies the safety regulations using a volume of fluid (VOF) model that analyzes multiphase flow through a surface tracking technique. Nitric acid and sulfuric acid were used in the study; they were selected because they are frequently involved in leakage accidents. In the event of a leak in a liquid tank, a dike with a deflector plate was very effective in reducing overtopping, and a deflector at a 45° angle was more effective than a 30° deflector. However, it is necessary to install additional safeguards at the joint between the dike and the deflection plate to withstand the force of the liquid.

Indium hydroxide (In(OH) 3 ) and indium oxide (In 2 O 3 ) have proven to be efficient catalysts for photocatalytic water-splitting reactions to produce hydrogen (H 2 ) and for organic pollutant degradation applications. However, the limited optical absorption features of indium-based nanostructures have restricted their practical applications. In this study, we have successfully designed indium hydroxide- and indium oxide-loaded metal sulfide (cadmium sulfide, CdS) heterostructures as excellent photocatalytic systems for photocatalytic hydrogen evolution and tetracycline hydrochloride pollutant degradation reactions. In this system, In(OH) 3 and In 2 O 3 established Type-I and S-scheme heterojunctions, respectively, with CdS, resulting in superior charge separation properties and outstanding photocatalytic activity. Specifically, the rational and appropriate design of the aforementioned indium-based heterostructures promoted the separation of photoexcited charge carriers via Type-I and S-scheme paths. Accordingly, enhanced photocatalytic H 2 evolution activities of 9.58 and 14.98 mmol·g -1 ·h -1 were achieved for CdS-In(OH) 3 and CdS-In 2 O 3 , respectively. Furthermore, the highest degradation efficiency of CdS-In 2 O 3 was ∼ 90%, which was higher than those of CdS-In(OH) 3 (72%) and bare CdS nanorods (51%). Therefore, the results of this study provide an opportunity to enhance the catalytic activities of heterostructured photocatalytic systems by utilizing the strategy of transitioning band structure alignment from the Type-I to the S-scheme., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

This paper reports a simple method of designing and synthesizing magnetic iron oxide (IO) integrated locust bean gum-cl-polyacrylonitrile hydrogel nanocomposites (LBG-cl-PAN/IONP) by in situ mineralization of iron ions in a hydrogel matrix. A two-step gel crosslink method followed by co-precipitation method was used to prepare these novel hydrogels embedded with magnetic iron oxide nanoparticles. The LBG-cl-PAN/IONP hydrogel nanocomposite (HNC) were tested in batch adsorption experiments for their ability to remove a cationic dyes, methylene blue (MB) & Methyl violet (MV), from aqueous solution. In order to analyze the LBG-cl-PAN/IONP HNC, FTIR, XRD, XPS, VSM, TEM, and EDX techniques were applied. Numerous operating parameters were studied, including the amount of adsorbent, the contact time, pH, temperature, the dye concentration, and the coexisting ion concentration. According to the Langmuir isotherm model, MB and MV had maximum monolayer adsorptive capacities of 1250 and 1111 mg/g, respectively. LBG-cl-PAN/IONP HNC controlled IONP oxidation as well as sustained adsorptive removal over a wide pH range (7-10). The key mechanism of adsorption consisted of electrostatic interaction and ion exchange. For successful use in successive cycles after regeneration using HNO 3 as eluent, the LBG-cl-PAN/IONP HNC can easily be reused. As a material, the LBG-cl-PAN/IONP HNC is a promising sorbent or composite material for removing toxic dyes from water, and therefore can be applied to enhance water and wastewater treatment technology. Additionally, we have briefly evaluated LBG-cl-PAN/IONP HNC for antibacterial and supercapacitor applications. According to our knowledge, this is the first report describing the use of LBG-cl-PAN/IONP HNC multifunctional efficacy as an excellent sorbent, antibacterial and electrochemical supercapacitor applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Earth abundant metal based plasmonic photocatalysis is one of the most proficient approaches to degrade the emergent organic pollutants in contaminated water. Here, we report that using one-dimensional CdS/zero-dimensional Bi quantum dot (QD) heterostructures (1D/0D CdS/Bi HSs) were obtained via a simple solvothermal reaction. The results specified that the Bi QDs were grown onto CdS NRs through the reduction of Bi 3+ ions. The Bi modified CdS HSs were employed as a photocatalyst for pharmaceutical pollutant tetracycline degradation and the optimized sample showed the maximum photocatalytic degradation activity of 90% under visible light radiation within 60 min, which is greater than the pure CdS (52%) under identical conditions. Based on the structural characterizations and degradation efficiency, the obtained CdS/Bi is a promising photocatalyst for the treatment of wastewater which contains emerging pollutants such as organic dyes and pharmaceutical antibiotics during the industrial processes. The boosted photocatalytic degradation efficiency is credited to the doped Bi 3+ species; surface plasmon resonance effect that raised from metallic Bi QDs and proficient photoinduced charge carriers separation., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The number of meiotic crossovers is tightly controlled and most depend on pro-crossover ZMM proteins, such as the E3 ligase HEI10. Despite the importance of HEI10 dosage for crossover formation, how HEI10 transcription is controlled remains unexplored. In a forward genetic screen using a fluorescent crossover reporter in Arabidopsis thaliana, we identify heat shock factor binding protein (HSBP) as a repressor of HEI10 transcription and crossover numbers. Using genome-wide crossover mapping and cytogenetics, we show that hsbp mutations or meiotic HSBP knockdowns increase ZMM-dependent crossovers toward the telomeres, mirroring the effects of HEI10 overexpression. Through RNA sequencing, DNA methylome, and chromatin immunoprecipitation analysis, we reveal that HSBP is required to repress HEI10 transcription by binding with heat shock factors (HSFs) at the HEI10 promoter and maintaining DNA methylation over the HEI10 5' untranslated region. Our findings provide insights into how the temperature response regulator HSBP restricts meiotic HEI10 transcription and crossover number by attenuating HSF activity., (© 2022 The Authors.)

In this work, we tailor facile hydrogels nanocomposite (HNC) based on sustainable karaya gum for water treatment. Karaya gum crosslink poly(acrylamide-co-acrylonitrile) @ silver nanoparticle (KG-cl-P(AAm-co-AN)@AgNPs) HNC were made by an aqueous free radical in situ crosslink copolymerization of acrylamide (AAm) and acrylic acid (AA) in aqueous solution of KG-stabilized AgNPs. FTIR, XRD, DTA-TGA, SEM, and TEM were used to characterize HNC. The hydrogels' swelling, diffusion, and network characteristics were investigated. The removal efficiency of HNC was found to be 99% at pH 8 for a crystal violet (CV), dose of 0.02 g after 1 h. Dye adsorption by these hydrogels was also investigated in terms of isotherms, and kinetics. The dye's exceptionally high adsorption capacity on HNC for CV removal is explained by H-bonding interactions, as well as dipole-dipole and electrostatic interactions between anionic adsorbent and cationic dye molecules (Qmax, 1000 mg/g). The HNC can be regenerated with 0.1 M HCl and reused at least 10 times maintaining over 68% dye removal. The loading of AgNPs into the polymeric matrix of KG-cl-P(AAm-co-AN) significantly increases the removal percentage of CV dye from its aqueous solution, according to this study., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Ternary heterostructures play a crucial role in improving the separation of charge carriers and fast surface reaction kinetics, which in turn helps in understanding the effective photocatalytic water splitting performance. Herein, CuS/Ag 2 O nanoparticles were presented on a graphitic carbon nitride (g-C 3 N 4 ) surface to obtain CuS/Ag 2 O/g-C 3 N 4 material using facile hydrothermal and precipitation methods. Structural and morphological studies confirmed the presence of ternary nanostructures comprising CuS, Ag 2 O, and g-C 3 N 4 with nanoparticle and nanosheet morphologies. The as-synthesized CuS/Ag 2 O/g-C 3 N 4 exhibited a remarkable photocatalytic H 2 production of 1752 µmol.h -1 .g -1 cat N 3 N 4 . The improved H 2 production performance which is due to the effective interfacial CuS/Ag 2 N 3 N 4 heterojunction interface and superior hole (h + ) trapping capability of the CuS at the CuS/Ag 2 N 3 N 4 interface. This can efficiently enhance the lifetime of photoexcited charge carriers and enhance the electron density for the production of H 2 . The optimum CuS/Ag 2 N 3 N 4 heterostructure remained stable after 8 successive experimental cycles, although with a slight change in the H 2 production rate. Therefore, this study offers a novel approach to exploit the efficacy through the synergetic effect of integrating CuS as the photocatalyst and Ag 2 O as the visible sensitizer, thus proposing a viable strategy of using earth-abundant material to enhance the conversion of solar energy to fuel., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)

The purpose of this study was to evaluate the defined daily doses (DDD)/1000 prescriptions/month (DPM) as a new indicator that can be used in pharmacies, and to describe antimicrobial use patterns in pharmacies nationwide in Japan. Dispensing volumes, number of prescriptions received, and facility information were obtained from 2638 pharmacies that participated in a survey. DPM was calculated based on the dispensing volume and number of prescriptions, which are routinely collected data that are simple to use. Use of third-generation cephalosporins, quinolones, and macrolides in pharmacies that received prescriptions primarily from hospitals or clinics decreased from January 2019 to January 2021. In particular, the antimicrobial use was higher in otorhinolaryngology departments than in other departments, despite a decrease in the antimicrobial use. In the linear multiple regression analysis, otorhinolaryngology department was independently associated with the third-generation cephalosporin, quinolone, and macrolide prescription in all periods. This study reveals for the first-time trends in antimicrobial use through a new indicator using the volume of drugs dispensed in pharmacies throughout Japan. Antimicrobial use differed by the medical department, suggesting the need to target interventions according to the department type.

Background: Recent studies have raised concern about the association of fluoroquinolones with an increased risk of aortic aneurysm and aortic dissection. We aimed to evaluate such risk in a Korean population., Methods: We conducted a nested case-control study using data from the National Health Insurance Service collected from 2013 to 2017 in Korea. The study cohort included patients older than 40 years and excluded patients who had used fluoroquinolones or been diagnosed with aortic aneurysm, aortic dissection, or related diseases 1 year prior to the cohort entry date. We randomly matched four controls in the risk set with each case of aortic aneurysm and aortic dissection (same sex, age, and cohort entry date). We assessed the risk of aortic aneurysm and aortic dissection from fluoroquinolones and adjusted for potential confounders using a conditional logistic regression model., Results: A total of 29,638 aortic aneurysm and aortic dissection patients were identified between 2014 and 2017. The use of fluoroquinolones within a year was associated with a 10% increased risk of aortic aneurysm and aortic dissection (adjusted odds ratio: 1.10, 95% CI 1.07-1.14, p < 0.05) compared with nonusers. The risk was higher in patients who had used fluoroquinolones within 60 days (adjusted odds ratio: 1.53, 95% CI 1.46-1.62, p < 0.05). The risk of aortic aneurysm and aortic dissection positively correlated with the cumulative dose and duration of fluoroquinolone therapy (p < 0.001)., Conclusions: Our study provides real-world evidence of the risk of aortic aneurysm and aortic dissection from fluoroquinolones in Korea. Patients and medical professionals should be aware that fluoroquinolones can increase the risk of aortic aneurysm and aortic dissection, which may be acerbated by high dosage and duration of use., (© 2022. The Author(s).)

Designing efficient ternary nanostructures is a feasible approach for energy production under simulated solar irradiation. In this study, excellent photoexcited charge carrier separation and enhanced visible-light response were achieved with nitrogen-doped titania nanobelts (N-TNBs), whose 1D geometry facilitated the fabrication of a heterostructure with SnS 2 on the surface of graphitic carbon nitride (g-C 3 N 4 ). We established the design of SnS 2 @N-TNB and SnS 2 @N-TNB/g-C 3 N 4 heterostructures by in situ hydrothermal and ultrasonication processes, and achieved commendable simulated solar light driven photocatalytic H 2 generation. UV-vis diffuse reflectance spectroscopy analysis revealed a red shift in the absorption spectra of the SnS 2 @N-TNB and SnS 2 @N-TNB/g-C 3 N 4 samples. The H 2 produced via SnS 2 @N-TNB-10/g-C 3 N 4 (6730.8 µmol/g/h) was 2.6 times higher than that produced by SnS 2 @N-TNB (2515.1 µmol/g/h), and 299 times higher than that produced by N-TNB (22.5 µmol/g/h). The improved photocatalytic H 2 production was attributed to the maximum interface contact between SnS 2 @N-TNB and g-C 3 N 4 , and to the improved visible-light absorption and effective charge-carrier separation. Therefore, the present study provides novel insights for combining the advantages of ternary materials to improve the conversion of solar energy to H 2 fuel., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Little is known about the modulatory capacity of the microbiota in early intestinal development. We examined various intestinal models that respond to gut microbial metabolites based on human pluripotent stem cell-derived human intestinal organoids (hIOs): physiologically relevant in vitro fetal-like intestine, intestinal stem cell, and intestinal disease models. We found that a newly isolated Limosilactobacillus reuteri strain DS0384 accelerated maturation of the fetal intestine using 3D hIO with immature fetal characteristics. Comparative metabolomic profiling analysis revealed that the secreted metabolite N-carbamyl glutamic acid (NCG) is involved in the beneficial effect of DS0384 cell-free supernatants on the intestinal maturation of hIOs. Experiments in an intestinal stem cell spheroid model and hIO-based intestinal inflamed model revealed that the cell-free supernatant from DS0384 comprising NCG promoted intestinal stem cell proliferation and was important for intestinal protection against cytokine-induced intestinal epithelial injury. The probiotic properties of DS0384 were also evaluated, including acid and bile tolerance and ability to adhere to human intestinal cells. Seven-day oral administration of DS0384 and cell-free supernatant promoted the intestinal development of newborn mice. Moreover, NCG exerted a protective effect on experimental colitis in mice. These results suggest that DS0384 is a useful agent for probiotic applications and therapeutic treatment for disorders of early gut development and for preventing intestinal barrier dysfunction.

Photocatalysis is a promising approach for generating hydrogen, an eco-friendly and cost-effective fuel. It is hypothesized that the ternary catalyst ZnIn 2 S 4 -rGO-CuInS 2 , prepared by ultrasonication method, should be effective for optimized photocatalytic hydrogen generation in a Na 2 S/Na 2 SO 3- water mixture. The as-synthesized catalyst was characterized using various surface analytical and optical techniques. Field-emission scanning electron microscopy and high-resolution transmission electron microscopy analyses revealed that marigold-like structured ZnIn 2 S 4 and layer-structured CuInS 2 were dispersed on the reduced graphene oxide sheets. The ternary ZnIn 2 S 4 -rGO-CuInS 2 system showed enhanced photocatalytic H 2 production compared to pure ZnIn 2 S 4 , CuInS 2 , ZnIn 2 S 4 -rGO, CuInS 2 -rGO, and ZnIn 2 S 4 -CuInS 2 catalysts under visible light illumination. The fabricated ZnIn 2 S 4 -rGO-CuInS 2 catalyst afforded hydrogen generation of 2531 μmol/g after 5 h. The enhanced performance of the ZnIn 2 S 4 -rGO-CuInS 2 catalyst originates from the synergetic effect with rGO as the electron transfer medium, and is confirmed by photocurrent density and photoluminescence measurements that indicate reduced recombination between the excited electron and hole pairs, and fast electron transfer in the ternary composite. The excellent performance of the ZnIn 2 S 4 -rGO-CuInS 2 catalyst for up to three consecutive cycles was demonstrated in cyclic stability tests under visible-light illumination., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Abiotic stress, a serious threat to plants, occurs for extended periods in nature. Abscisic acid (ABA) plays a critical role in abiotic stress responses in plants. Therefore, stress responses mediated by ABA have been studied extensively, especially in short-term responses. However, long-term stress responses mediated by ABA remain largely unknown. To elucidate the mechanism by which plants respond to prolonged abiotic stress, we used long-term ABA treatment that activates the signalling against abiotic stress such as dehydration and investigated mechanisms underlying the responses. Long-term ABA treatment activates constitutive photomorphogenic 1 (COP1). Active COP1 mediates the ubiquitination of golden2-like1 (GLK1) for degradation, contributing to lowering expression of photosynthesis-associated genes such as glutamyl-tRNA reductase (HEMA1) and protochlorophyllide oxidoreductase A (PORA), resulting in the suppression of chloroplast development. Moreover, COP1 activation and GLK1 degradation upon long-term ABA treatment depend on light intensity. Additionally, plants with COP1 mutation or exposed to higher light intensity were more sensitive to salt stress. Collectively, our results demonstrate that long-term treatment of ABA leads to activation of COP1 in a light intensity-dependent manner for GLK1 degradation to suppress chloroplast development, which we propose to constitute a mechanism of balancing normal growth and stress responses upon the long-term abiotic stress., (© 2021 John Wiley & Sons Ltd.)

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome., (© 2021. The Author(s).)

Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among the currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful of RBPs known to enhance MT and several others reported to suppress MT, leaving the global impact of RBPs on MT elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 human RBPs and evaluated the quantitative effect of individual RBPs on MT efficacy. In contrast to previous studies, we show that most RBPs significantly affect MT and that all of those MT-regulating RBPs function as MT enhancers rather than suppressors, by making the local secondary structure of the target site accessible to Argonaute. Our findings illuminate the unappreciated regulatory impact of human RBPs on MT, and as these RBPs may play key roles in the gene regulatory network governed by metazoan miRNAs, MT should be understood in the context of co-regulating RBPs., (© 2021. The Author(s).)

Photocatalytic removal is more appropriate for the destruction of organic contaminants. The ternary Bi 2 MoO 6 -reduced graphene oxide (rGO)-TiO 2 catalyst was synthesized using a simple hydrothermal method, and various surface analytical optical techniques were analyzed. The photocatalytic decomposition efficiency of the Bi 2 MoO 6 -rGO-TiO 2 composite was 92.3% higher than those of pure and binary photocatalysts. The effects of operational parameters, such as catalyst ratio, catalyst variation, rGO ratio variation, and pH value variation were also analyzed. The as-prepared ternary photocatalyst exhibited low photoluminescence and high photocurrent density, which suppressed photon-induced electron and hole (h + ) recombination and effective charge separation. The study demonstrated that rGO has excellent electron transfer performance and enhanced photocatalytic reaction stability. The perfect cycling stability of Bi 2 MoO 6 -rGO-TiO 2 was retained even after five consecutive cycles on the photocatalytic degradation reaction performance. In this study, we propose a decomposition performance mechanism for ofloxacin degradation that underwent visible-light irradiation., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Bimetallic alloy nanospheres hybridized with semiconductor square-shaped discs are promising catalysts for photocatalytic water splitting, because they exhibit multicomponent interactions, high catalytic activity, and stability. Herein, Cu-Pd/N-Bi 2 WO 6 heterostructures consisting of bimetallic Cu-Pd alloy nanospheres uniformly dispersed on N-Bi 2 WO 6 square-shaped discs are reported. The as-prepared 1 wt% Cu-Pd/N-Bi 2 WO 6 catalyst exhibits a higher H 2 production rate (4213 µmol/g) under simulated solar light illumination than N-Bi 2 WO 6 (291 µmol/g). The considerably high H 2 production rate is ascribed to the exposed catalytically active sites of the Cu-Pd alloy nanospheres, which facilitate the formation of rapid charge transfer channels between Cu-Pd and N-Bi 2 WO 6 . Moreover, the photocatalyst stability is improved by aggregation of the highly dispersed Cu-Pd alloy nanospheres on the N-Bi 2 WO 6 surface. Accordingly, a reaction mechanism based on the work functions of the bimetallic Cu-Pd alloy nanospheres and N-Bi 2 WO 6 square-shaped discs is proposed to elucidate the photocatalytic reaction pathway. The holes (which accumulate in the N-Bi 2 WO 6 square-shaped discs) and Pd (which acts as an electron channel) can effectively inhibit the recombination of charge carriers, and Cu (which acts as the cocatalyst) can synergistically increase the H + reduction rate. This study provides a new effective route for the design of high-performance heterostructures for efficient photocatalytic H 2 production., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Objective: The study aims to understand the relationship between the living-alone period and depressive symptoms among the elderly in South Korea., Methods: The study obtained data on the elderly over 65 years old from the 2006 to 2019 Korea Welfare Panel Study. Generalized estimating equation (GEE) analysis was performed to understand changes in depressive symptoms over periods of living alone. Additional subgroup analysis of age and gender was conducted to observe differences., Results: A total 568 (19% of the study samples) elderly transitioned from living with others into a living-alone state. Males and females showed a significant increase in depressive symptoms as they started to live alone, which gradually decreased with prolonged periods of living alone. The result of the GEE analysis demonstrated high odds of depressive symptoms during the first (OR = 1.857; 95% CI: 1.492-2.310), second (OR = 1.414; 95% CI: 1.127-1.774), and third (OR = 1.413; 95% CI: 1.105-1.808) years of living alone compared with living with others. Regarding additional subgroup analysis, the female and old-old groups showed high odds of depressive symptoms in the first 3 years of living alone, whereas the male and young-old groups showed high odds for the first year only., Conclusions: The study provides valuable insights into the relationship between the living-alone period and depressive symptoms among the elderly. Future research using the living-alone period on various social factors are recommended., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Recently, the frenetic development of stable quaternary material with a wide range of solar energy absorption and separation of charge carrier has emerged as a favorable material for the solar-to-hydrogen conversion. In this work, quaternary CuS-AgVO 3 /Ag-TNR heteronanostructures (HNSs) synthesized by an ultra-sonication method for stabilized solar light photocatalytic hydrogen production in glycerol-water mixture. Among the prepared photocatalysts, the 1 wt% CuS-AgVO 3 /Ag-TNR HNS produced the highest H 2 activity (756 µmol/g), approximately 84 times greater than the TNR due to higher charge separation, excellent conductivity, plasmonic resonance effect, and electron-storing capacity. Interestingly, the accelerated charge transfer pathway through the Schottky junction between the AgVO 3 and Ag to the conduction band of the TNR and thereafter to the electron acceptor of CuS for the reduction of H + ions to H 2 . Additionally, a possible photocatalytic mechanism of CuS-AgVO 3 /Ag-TNR HNS for improved H 2 production was proposed based on the results obtained by various characterization techniques. Therefore, present research work explores the new insights to design high-performance CuS-AgVO 3 /Ag-TNR HNS material for the conversion of clean renewable H 2 energy for the futuristic transport applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Inc. All rights reserved.)

In order to improve the electrochemical performance of the NiCo 2 O 4 material, Ni ions were partially substituted with Cu 2+ ions having excellent reducing ability. All of the electrodes were fabricated by growing the Ni 1-x Cu x Co 2 O 4 electrode spinel-structural active materials onto the graphite felt (GF). Five types of electrodes, NiCo 2 O 4 /GF, Ni 0.875 Cu 0.125 Co 2 O 4 /GF, Ni 0.75 Cu 0.25 Co 2 O 4 /GF, Ni 0.625 Cu 0.375 Co 2 O 4 /GF, and Ni 0.5 Cu 0.5 Co 2 O 4 /GF, were prepared for application to the oxygen evolution reaction (OER). As Cu 2+ ions were substituted, the electrochemical performances of the NiCo 2 O 4 -based structures were improved, and eventually the OER activities were also greatly increased. In particular, the Ni 0.75 Cu 0.25 Co 2 O 4 /GF electrode exhibited the best OER activity in a 1.0 M KOH alkaline electrolyte: the cell voltage required to reach a current density of 10 mA cm -2 was only 1.74 V (η = 509 mV), and a low Tafel slope of 119 mV dec -1 was obtained. X-ray photoelectron spectroscopy (XPS) analysis of Ni 1-x Cu x Co 2 O 4 /GF before and after OER revealed that oxygen vacancies are formed around active metals by the insertion of Cu ions, which act as OH-adsorption sites, resulting in high OER activity. Additionally, the stability of the Ni 0.75 Cu 0.25 Co 2 O 4 /GF electrode was demonstrated through 1000th repeated OER acceleration stability tests with a high faradaic efficiency of 94.3%.

One dimensional (1D) metal sulfide nanostructures are one of the most promising materials for photocatalytic water splitting reactions to produce hydrogen (H 2 ). However, tuning the nanostructural, optical, electrical and chemical properties of metal sulfides is a challenging task for the fabrication of highly efficient photocatalysts. Herein, 1D CdS nanorods (NRs) were synthesized by a facile and low-cost solvothermal method, in which reaction time played a significant role for increasing the length of CdS NRs from 100 nm to several micrometers. It is confirmed that as the length of CdS NR increases, the visible photocatalytic H 2 evolution activity also increases and the CdS NR sample obtained at 18 hr. reaction time exhibited the highest H 2 evolution activity of 206.07 μmol.g -1 .h -1 . The higher H 2 evolution activity is explained by the improved optical absorption properties, enhanced electronic bandstructure and decreased electron-hole recombination rate.

Key Message: We produced a biologically active phage-encoded endolysin, LysP11, in N. benthamiana. Plant-produced LysP11 exhibited robust antimicrobial activity against E. rhusiopathiae, and C-terminal domain of LysP11 bound specifically to E. rhusiopathiae. Bacterial resistance to antibiotics, a serious issue in terms of global public health, is one of the leading causes of death today. Thus, new antimicrobial agents are needed to combat pathogens. Recent research suggests that bacteriophages and endolysins derived from bacteriophages are potential alternatives to traditional antibiotics. Here, we examined the antimicrobial activity of LysP11, which is encoded by Propionibacterium phage P1.1 and comprises an N-terminal amidase-2 domain and a C-terminal domain with no homology to other bacteriophage endolysins. LysP11 was produced in Nicotiana benthamiana (N. benthamiana) using an Agrobacterium-mediated transient expression strategy. LysP11 was purified on microcrystalline cellulose-binding resin after attachment of the Clostridium thermocellum-derived family 3 cellulose-binding domain as an affinity tag. The affinity tag was removed using the small ubiquitin-related modifier (SUMO) domain and SUMO-specific protease. Plant-produced LysP11 showed strong antimicrobial activity toward Erysipelothrix rhusiopathiae (E. rhusiopathiae), mediated via lysis of the cell wall. Lytic activity was optimal at pH 8.0-9.0 (37 °C) and increased at higher concentrations of NaCl up to 400 mM. Furthermore, the C-terminal domain of LysP11 bound specifically to the E. rhusiopathiae cell wall. Based on these results, we propose that LysP11 is a potential candidate antimicrobial agent against E. rhusiopathiae.

The minerals have a somewhat slower than other transition metals at critical reduction rates in their ability to deliver oxygen. Thus, single minerals alone do not exhibit a higher oxygen transfer capacity than metal oxide oxygen carriers. In this study, we try to solve the problem of single mineral ilmenite (FeTiO₃) by combining it with Fe-based red mud and Cu oxide. When the ilmenite was used without calcination, the CH₄-CO/air redox cycle showed rapid decayed. However, when ilmenite was calcined, the CH4-CO/air redox cycle became stable, and the oxygen transfer rate increased to 4.2%. This is because the FeTiO₃ structure was converted to the pseudobrookite (Fe₂TiO 5 ) structure through the calcination process. That is, the Fe 2+ ion in the ilmenite structure was converted into an Fe 3+ ion. When 30 wt.% of red mud was added to the Fe ion, it reacted with the rutile-type titania mixed with pseudobrookite-typed Fe₂TiO 5 , producing an almost perfect pseudobrookite crystal. This resulted in a slight increase in the capacity of oxygen transfer to 4.9%. When 15 wt.% of Cu oxide was added, the oxygen transfer capacity increased to 6.0%. This performance was indicated by the cyclic voltammetry curve that remained constant even after 200 cycles. Here, we argue that if low-cost minerals as a base material are used in appropriate amounts, the production of a lowest-cost oxygen carrier can be achieved.

An electronic health record (EHR) contains various clinical information for pharmacovigilance studies, but they remain difficult to use. From 2016 to 2018, the ministry of food and drug safety and Korea institute of drug safety & risk management (KIDS) converted the EHRs of more than 9 million patients to a MOA common data model (CDM). KIDS developed the Medical record observation and assessment for drug safety network (MOA-Net), a web portal site to build a network between CDM data partners. Through MOA-Net, hospitals participated in pharmacovigilance studies and confirmed the usability and adequacy of the CDM.

Background: Diet plays an important role in both the development and management of diabetes., Objective: Using data from the Korean Genome Epidemiology Study, we assessed dietary patterns associated with the clinical indicators of diabetes., Methods: This study included 7255 subjects aged 40-69 y. Individuals with chronic diseases were excluded. The daily intakes of specific food items were assessed using a dish-based semiquantitative food-frequency questionnaire comprising 103 items; the food items were then grouped into 26 food groups. Dietary patterns were analyzed by the reduced rank regression method using glycated hemoglobin, the homeostasis model of insulin resistance, and fasting glucose concentrations as dependent variables. We investigated the associations between dietary patterns and incident diabetes using the Cox proportional hazards model., Results: During an 11.5-y follow-up, the incidence of diabetes was 11.8/1000 person-years. The dietary pattern related to selected biomarkers of diabetes was characterized by a relatively high intake of kimchi, beef, other meat, fish, and coffee in men and a high intake of rice, kimchi, and fruit in women. In men, the association of dietary patterns with incident diabetes was significant only in the obese group, and those in the top quartile of the dietary pattern score had a 1.72 times (95% CI: 1.15, 2.56 times) greater risk of incident diabetes than those in the bottom quartile. Conversely, dietary patterns in women were not associated with incident diabetes., Conclusion: Using reduced rank regression, we identified dietary patterns related to selected biomarkers of diabetes in a long-term study with follow-up data in Korea., (© 2018 American Society for Nutrition.)

Egg products are widely consumed in Korea and continue to be associated with risks of Staphylococcus aureus -induced food poisoning. This prompted the development of predictive mathematical models to understand growth kinetics of S. aureus in egg products in order to improve the production of domestic food items. Egg products were inoculated with S. aureus and observe S. aureus growth. The growth kinetics of S. aureus was used to calculate lag-phase duration (LPD) and maximum specific growth rate (µ max ) using Baranyi model as the primary growth model. The secondary models provided predicted values for the temperature changes and were created using the polynomial equation for LPD and a square root model for µ max . In addition, root mean square errors (RMSE) were analyzed to evaluate the suitability of the mathematical models. The developed models demonstrated 0.16-0.27 RMSE, suggesting that models properly represented the actual growth of S. aureus in egg products.

Background: Obesity can lead to psychological, social, and medical problems that may negatively affect the quality of life Aim: In our study, we aimed to evaluate the body perception, psychological distress, and subjective quality of life of obese subjects in comparison with normal weighted ones., Methods: A total of 494 subjects, aged between 18 and 64 years, were included to the study. Patients with the body mass index (BMI) of ≥18.5-24.9 kg/m2 were assigned to the control group; BMI ≥ 30 kg/m2 were assigned to the obese patient group. An ethical committee approval with protocol No. of 2011/242 was obtained from the Ethical Committee of Eskişehir Osmangazi University Faculty of Medicine. Interviews were performed two times with obese subjects in the 1st and the 6th month of weight loss program, once with the control group. Data identification form, the form which included the biochemical parameters, Trait Anxiety inventory, State anxiety scale, Quality of Life scale-Short Form [(SF-36)(QOL)], Multidimensional Body-Self Relations Questionnaire (MBSRQ), Zung Depression Rating Scale, and Zung Anxiety Rating Scale were applied to participants., Results: Obesity is more common in women. Obesity reduces the quality of life, negatively affects body perception, and triggers anxiety. Because of their body structure, obese individuals are exposed to social pressure and unethical attitudes., Conclusions: Both health risks and social implications of obesity are important. Further studies are needed to ethically evaluate the psycho-social problems caused by obesity.

We investigated the subdomain structures of single-layer graphene oxide (GO) by characterizing local friction and conductance using conductive atomic force microscopy. Friction and conductance mapping showed that a single-layer GO flake has subdomains several tens to a few hundreds of nanometers in lateral size. The GO subdomains exhibited low friction (high conductance) in the sp(2)-rich phase and high friction (low conductance) in the sp(3)-rich phase. Current-voltage spectroscopy revealed that the local current flow in single-layer GO depends on the quantity of hydroxyl and carboxyl groups, and epoxy bridges within the 2-dimensional carbon layer. The presence of subdomains with different sp(2)/sp(3) carbon ratios on a GO flake was also confirmed by chemical mapping using scanning transmission X-ray microscopy. These results suggest that spatial mapping of the friction and conductance can be used to rapidly identify the composition of heterogeneous single-layer GO at nanometer scale, which is essential for understanding charge transport in nanoelectronic devices.

This study reports a method for the facile and high-yield exfoliation of WX2 (X = S, Se) by sonication under aqueous conditions using single-stranded DNA (abbreviated as ssDNA) of high molecular weight. The ssDNA provided a high degree of stabilization and prevented reaggregation, and it enhanced the exfoliation efficiency of WX2 nanosheets due to adsorption on the WX2 surface and the electrostatic repulsion of sugars in the ssDNA backbone. The exfoliation yield was higher with ssDNA (80%-90%) than without (2%-4%); the yield with ssDNA was also higher than the value previously reported for aqueous exfoliation (∼10%). Given that two-dimensional nanomaterials have potential health and environmental applications, we investigated antibacterial activity of exfoliated WX2-ssDNA nanosheets, relative to graphene oxide (GO), and found that WSe2-ssDNA nanosheets had higher antibacterial activity against Escherichia coli K-12 MG1655 cells than GO. Our method enables large-scale exfoliation in an aqueous environment in a single step with a short reaction time and under ambient conditions, and it can be used to produce surface-active or catalytic materials that have broad applications in biomedicine and other areas.

Serum and liver metabolites in rats fed red ginseng (RG) were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The mass data were analyzed by partial least squares-discriminant analysis (PLS-DA) to discriminate between control and RG groups and identify metabolites contributing to this discrimination. The RG group was clearly separated from the control group on PLS-DA scores plot for serum samples, but not liver samples. The major metabolites contributing to the discrimination included lipid metabolites (lysophosphatidylcholine, acyl-carnitine, and sphingosine), isoleucine, nicotinamide, and corticosterone in the serum; the blood levels of all but isoleucine were reduced by RG administration. Not all metabolites were positively correlated with the health benefits of RG. However, the blood levels of lysophosphatidylcholine, which stimulate various diseases, and long-chain acylcarnitines and corticosterone, which activate the stress response, were reduced by RG, suggesting long-term RG might relieve stress and prevent physiological and biological problems.

We analyzed metabolites from a 50% aqueous methanol extract of green teas treated with different shade periods (0, 15, 18, and 20 days) to investigate the effect of low light on their nutritional and sensory qualities. The shaded groups could be clearly distinguished from the control (0 day), and the 20 day group was separated from the 15 and 18 day groups. The shade treatment increased quercetin-galactosylrutinoside, kaempferol-glucosylrutinoside, epicatechin gallate, epigallocatechin gallate, tryptophan, phenylalanine, theanine, glutamine, glutamate, and caffeine levels but decreased quercetin-glucosylrutinoside, kaempferol-glucoside, gallocatechin, and epigallocatechin levels. Further studies on the nutritional benefits of these metabolites are needed. However, this result, along with the sensory evaluation and color measurement data, suggests that shade treatment improves the nutritional and sensory quality of green tea. Thus, we proposed a metabolomic pathway related to the effect of low light, which could elucidate the relationship between low light and tea quality.

Objective: This study evaluated the frequencies of allergic symptoms and rate of upper respiratory infections during the past year in the general population, kitchen workers (KW) and health care workers (HCW)., Methods: The European Community Respiratory Health Survey (ECRHS) was used to inquire retrospectively about asthma and asthma-like symptoms and the number of treatments required for previous upper respiratory tract infections (URTI: acute pharyngitis, acute sinusitis, etc.) during the past year for health care workers, kitchen workers, and members of the general population. Adjusted odds ratios by gender, age, and smoking status were calculated., Results: 579 subjects (186 from the general population, 205 KW, and 188 HCW; 263 females, 316 males) participated in the study. Noninfectious (allergic) rhinitis was significantly higher in the HCW and KW groups than in the general population (P < 0.001). Cumulative asthma was significantly higher only in the HCW group (P < 0.05). In addition, the HCW and KW groups had significantly higher risks of ≥2/year URTI (OR: 1.59, 95% CI: 1.07-2.38 versus OR: 1.57, 95% CI: 1.05-2.38) than the general population., Conclusion: Occupational allergic respiratory diseases are an important and growing health issue. Health care providers should become familiar with workplace environments and environmental causes of occupational rhinitis and asthma.

We used UPLC-Q-TOF MS to analyze hepatic metabolites of rats aged 6, 12, 18, and 24 months; the MS data were processed by partial least-squares discriminant analysis (PLS-DA) to investigate the discrimination among sample groups. Rats were significantly separated with increasing age, except those aged between 6 and 12 months. We identified only 25 of 120 metabolites contributing to the separation: lipid metabolites (glycerol-3-phosphate, linolenic acid, lysophosphatidylcholines [lysoPCs]), energy metabolism intermediates (betaine, carnitine, acylcarnitines, creatine, pantothenic acid), nucleic acid metabolites (inosine, xanthosine, uracil, hypoxanthine, xanthine), and tyrosine. Aging accumulated energy metabolism intermediates, hypoxanthine, xanthine, and 2 major lysoPCs (C18:0 and C22:6). The NAD level and NAD/NADH ratio decreased with age. It was indicated that aging might decrease energy production through β-oxidation because of a decrease in NAD despite the accumulation of lipid energy metabolism intermediates. In addition to energy dysregulation, hypoxanthine and xanthine, which are elevated with age, might accumulate reactive oxygen species in the liver. These results strongly support two aging theories: those of energy dysregulation and free radicals. Additionally, we propose a metabolic pathway related to aging based on these hepatic metabolites. These metabolites and the proposed aging pathway could be used to understand aging and related diseases better, and increase the predictability of aging risk.

Objective: There are several pathways that mediate the aberrant metabolism of glucose and that might induce greater vascular damage in the setting of diabetes. The polyol pathway mediated by aldose reductase (AR) has been postulated to be one such pathway. However, it has been reported that AR reduces toxic lipid aldehydes and, under some circumstances, might be antiatherogenic., Methods and Results: Atherosclerosis development was quantified in 2 lines of transgenic mice expressing human AR (hAR) crossed on the apolipoprotein E knockout background. The transgenes were used to increase the normally low levels of this enzyme in wild-type mice. Both generalized hAR overexpression and hAR expression via the Tie 2 promoter increased lesion size in streptozotocin diabetic mice. In addition, pharmacological inhibition of AR reduced lesion size., Conclusions: Although in some settings AR expression might reduce levels of toxic aldehydes, transgenic expression of this enzyme within the artery wall leads to greater atherosclerosis.

Background: There have been multiple studies carried out in many countries with regard to the use of nonvitamin, nonmineral (NVNM) supplements. These studies have shown that the use of NVNM supplements is on the increase throughout the world, particularly in western countries. The aim of this study was to assess the extent of NVNM supplement use among Turkish university students., Methods: The survey was conducted between September and December 2004 at Osmangazi University, a public university located in the west of Turkey. Responses were analysed, using the chi-square (x2) test, t test and percent (%) ratios, according to gender and consumers. Differences were considered significant for p