2,309 results on '"Muñoz S"'
Search Results
152. Comparación de sutura mecánica con sutura manual de la faringe en laringectomía total
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Osorio M., Jaime, primary, Morales A., Carlos, additional, Contreras R., Pilar, additional, Pineda D., Rodrigo, additional, Silva R., Fernanda, additional, Muñoz S., Daniel, additional, Suazo D., Angélica, additional, Cancino C., Miguel, additional, and Arregui V., Rodrigo, additional
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- 2021
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153. Feeding strategies of the copepod Acartia clausi on single and mixed diets of toxic and non-toxic strains of the dinoflagellate Alexandrium minutum
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Barreiro, A., Guisande, C., Frangópulos, M., González-Fernández, A., Muñoz, S., Pérez, D., Magadán, S., Maneiro, I., Riveiro, I., and Iglesias, P.
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- 2006
154. Rol protector de N-acetilcisteína en la ototoxicidad inducida por cisplatino en pacientes con cáncer de cabeza y cuello. Ensayo clínico controlado aleatorizado
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Muñoz S.,Daniel, Cardemil M.,Felipe, Readi S.,Andrés, and Contreras R.,José Miguel
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otoprotección ,ototoxicidad ,cisplatino ,hipoacusia ,N-acetilcisteina - Abstract
Resumen Introducción: La ototoxicidad por cisplatino es un evento muy frecuente y sus consecuencias producen mucho deterioro en los pacientes. El diagnóstico precoz es esencial, pues permitiría implementar apropiadamente estrategias para aminorar su efecto. Entre estas tenemos la N-acetilcisteína, un agente antioxidante que ha demostrado efecto otoprotector. Objetivo: Evaluar el rol otoprotector de N-acetilcisteína comparado con placebo, en pacientes con cáncer de cabeza y cuello tratados con cisplatino. Material y Método: Ensayo clínico aleatorizado, paralelo y controlado con placebo. Se incluyen pacientes con cáncer de cabeza y cuello que requieren tratamiento con cisplatino, dos ramas: un grupo control que recibe placebo y otro que recibe el fármaco. Se realizan audiometrías de altas frecuencias (6-16 kHz) antes, durante y una vez finalizado el tratamiento. Resultados: Se aleatorizaron 45 pacientes, 23 al grupo intervencional y 22 al grupo control. Se encontró una incidencia general de la ototoxicidad del 73%, un empeoramiento en relación con tiempo de medición, una detención y estabilización del efecto ototóxico en el grupo que recibió N-acetilcisteína, todas estas diferencias fueron significativas. Conclusión: La N-acetilcisteína no previene la ototoxicidad inducida por cisplatino, pero modifica su curso de instalación y progresión. No se registraron efectos adversos al uso del fármaco. El monitoreo audiológico precoz es esencial para identificar la ototoxicidad y ejercer acciones para modificar su curso y mejorar la calidad de vida.
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- 2021
155. A Survey-based Approach for Selecting the Stations and Links for a Rapid Transit Network
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Escudero, L. F. and Muñoz, S.
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- 2014
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156. Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts
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Talayero, P., Mancebo, E., Calvo-Pulido, J., Rodríguez-Muñoz, S., Bernardo, I., Laguna-Goya, R., Cano-Romero, F. L., García-Sesma, A., Loinaz, C., Jiménez, C., Justo, I., and Paz-Artal, E.
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- 2016
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157. Masonry mortar design incorporating crushed recycled glass.
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Muñoz, S. and Mendoza, S.
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MASONRY , *CONSTRUCTION materials , *RENEWABLE natural resources , *BRICKLAYERS , *MORTAR - Abstract
Mortar is considered one of the construction materials in great demand in the world, obtaining materials for its preparation require the exploitation of a large part of renewable and nonrenewable resources. In order to dissipate the consumption of natural aggregates, the reuse of different materials that are normally discarded has been studied; glass is an important option for the preparation of mortar. Therefore, the objective of the present investigation was to design a mortar for masonry incorporating crushed glass. The mixing design was made for dosages of 1: 3.5, 1: 4, 1: 5 and 1: 6 with substitution of 5%, 10%, 15%, 20%, 25% and 30% of fine aggregate by crushed glass; tested at the age of 7, 14, 21 and 28 days. The fluidity of the mortar in fresh state and the mechanical properties such as the resistance to compression and bending of the mortar, resistance to adhesion by bending in masonry piles, resistance to axial compression of piles and resistance to diagonal compression in walls were evaluated. of bricklayer. The highest resistance was obtained for the dosage of 1: 3.5 with 30% as the optimum percentage of substitution, reaching a higher resistance compared to the standard mortar; for the dosage of 1: 4 the optimum percentage was 25%; for 1: 5 it was determined with 20%; and finally, 10% was obtained as the best percentage for the 1: 6 dosages. From these values it was determined that, once the optimal percentages were reached, the strength of the mortar decreases as the substitution increases. The results obtained allowed to conclude that the crushed glass significantly influences the mortar properties. [ABSTRACT FROM AUTHOR]
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- 2022
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158. Medication reconciliation at hospital admission and discharge in an orthopedic surgery and traumatology department
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Franco-Donat, M., Soler-Company, E., Valverde-Mordt, C., García-Muñoz, S., Rocher-Milla, A., and Sangüesa-Nebot, M.J.
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- 2010
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159. Conciliación de la medicación al ingreso y al alta hospitalaria en un servicio de cirugía ortopédica y traumatología
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Franco-Donat, M., Soler-Company, E., Valverde-Mordt, C., García-Muñoz, S., Rocher-Milla, A., and Sangüesa-Nebot, M.J.
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- 2010
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160. Rentabilidad y seguridad de la biopsia renal percutánea en riñones nativos con aguja automática de 16G
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Fortuño Andrés, J.R., de Lamo Muñoz, S., García Pérez, E., and Mateos Álvarez, A.
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- 2010
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161. Aumento de la confianza en la interpretación del PET con 18F-Florbetaben: “machine learning” basado en la aproximación cuantitativa
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García Vicente, A.M., primary, Tello Galán, M.J., additional, Pena Pardo, F.J., additional, Amo-Salas, M., additional, Mondejar Marín, B., additional, Navarro Muñoz, S., additional, Rueda Medina, I., additional, Poblete García, V.M., additional, Marsal Alonso, C., additional, and Soriano Castrejón, Á., additional
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- 2021
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162. P06.06 Clinical Implications of Tumour Spread Through Air Spaces (STAS) In Lung Adenocarcinoma Patients Treated With Surgery
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Gutierrez Sainz, L., primary, Lopez Muñoz, S., additional, Cruz Castellanos, P., additional, Esteban Rodriguez, M.I., additional, Higuera, O., additional, and De Castro Carpeño, J., additional
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- 2021
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163. Rol protector de N-acetilcisteína en la ototoxicidad inducida por cisplatino en pacientes con cáncer de cabeza y cuello. Ensayo clínico controlado aleatorizado
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Muñoz S., Daniel, primary, Cardemil M., Felipe, additional, Readi S., Andrés, additional, and Contreras R., José Miguel, additional
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- 2021
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164. 344MO Experience of GEINO (Spanish cooperative group for research in neuro-oncology) oncologists in the management of adult medulloblastoma
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Vieito Villar, M., primary, Luque Caro, R., additional, Martin Soberon, M.C., additional, Herrero Ibañez, A., additional, Girones Sarrio, R., additional, Luque Caro, N., additional, Alonso Garcia, M., additional, Quintanar Verduguez, T., additional, del Barco Berrón, S., additional, Moya Horno, I., additional, Tuñón Cabeza, M.T., additional, Gallego, O., additional, de las Peñas, R., additional, Vaz Salgado, M.Á., additional, Ceballos Lenza, I., additional, Cunquero-Tomás, A.J., additional, Peralta Muñoz, S., additional, Gutierrez Toribio, M., additional, and Diaz Santos, J., additional
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- 2021
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165. 92P First-results of the CLIMB360 study, a prospective molecular screening program across multiple cancer types based on circulating tumor DNA (ctDNA)
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Garcia-Corbacho, J., primary, Victoria Ruiz, I., additional, Angelats, L., additional, Indacochea, A., additional, Martínez-Vidal, J., additional, Sole Bentz, P., additional, Moreno, D., additional, Faull, I., additional, Campillo, M.L., additional, Muñoz, S., additional, Saurí, T., additional, Reguart Aransay, N., additional, Viñolas, N., additional, Esposito, F.M., additional, Mezquita, L., additional, Basté, N., additional, Oliveres, H., additional, Castillo, S., additional, Nogué, M., additional, and Prat, A., additional
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- 2021
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166. LBA18 POSEIDON randomized phase II trial: Tamoxifen (TAM) + taselisib or placebo (PLA) in patients (pts) with hormone receptor positive (HR+)/HER2- metastatic breast cancer (MBC)
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Oliveira, M., primary, Baird, R.D., additional, Voorthuis, R.A.B., additional, De Boo, L., additional, van Rossum, A.G.J., additional, Garrigos Cubells, L., additional, Muñoz, S., additional, López-García, D., additional, Saura Manich, C., additional, Schrier, M., additional, Mandjes, I., additional, Sikorska, K., additional, Schot, M., additional, Kateb, A., additional, Mather, C., additional, Beelen, K., additional, Cortés, J., additional, Caldas, C., additional, and Linn, S.C., additional
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- 2021
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167. 1268P Real-time clinical utility of ctDNA genomic alterations in untreated patients with advanced NSCLC
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De Herreros, M. Garcia, primary, Muñoz, S., additional, Teixido, C., additional, Díez-Guardia, V., additional, Arcocha, A., additional, Pipinikas, C., additional, Martínez, D., additional, Castillo, S., additional, Reyes, R., additional, Melia, M. Riudavets, additional, Auclin, E., additional, Marin, E., additional, Howarth, K., additional, Martinez, D., additional, Puig, J.A., additional, Prat, A., additional, Aransay, N. Reguart, additional, Viñolas, N., additional, and Mezquita, L., additional
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- 2021
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168. Estimation of the through-plane thermal conductivity of polymeric ion-exchange membranes using finite element technique
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Barragán, V.M., primary, Izquierdo-Gil, M.A., additional, Maroto, J.C., additional, Antoranz, P., additional, and Muñoz, S., additional
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- 2021
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169. Involvement in Catalonia of the Anaesthesiology and Resuscitation Services in the SARS-CoV-2 pandemic. A real, cost-effective solution to an unprecedented health crisis
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Villalonga Vadell, R.M., Sala Blanch, X., Roigé Solé, J., Garcia Eroles, X., Morros Viñoles, C., Valero Castell, R., Bausili Ribera, M., Pedregosa Sanz, A., Sergi Angeles Fite, G., Abreu Paradell, S., Adalia Bartolomé, R., Álvarez Pérez, A., Amigó Bonet, N., Asbert Sagasti, R., Barbal Badia, F., Carramiñana Dominguez, A., Castellnou Ferré, J., Bernia Gil, J.A., Carol Boeris, F., Colomina Soler, M.J., Chamero Pastilla, A., del Moral García, M.V., del Pozo, D., Escolano Villén, F., Esquius Jofre, P., Ferrando Ortolá, C., García Marqueta, C., Hernández Aguado, C., Hernando Pastor, D., Isach Comallonga, N., Isern Domingo, X., Cesar Jaramillo Carrión, L., Koo García, M., Lacambra Basil, M., Lázaro Alcay, J.J., Mailan Bello, J., Manrique Muñoz, S., Martínez Palli, G., Martínez Jérez, L., Masdeu Castellví, J., Mercadal Mercadal, J., Montero Matamala, A., Morales Torres, O., Moret Ferron, M.L., Moret Ruiz, E., Muñoz Falcón, L., de Nadal Clanchet, M., Miquel Ortells Nebot, P., Prat Vallribera, A., Remartinez Fernandez, F., Roca Campos, P., Roldan Osuna, J., Sabaté Pes, A., Sala Francino, P., Saludes Serra, J., Samsó Sabe, E., Sarmiento Meneses, E., Soto Ejarque, J.M., Tomas Torrelles, A., Tomás Esteban, A., and Yuste Graupera, M.
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- 2021
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170. Implicación en Cataluña de los Servicios de Anestesiología y Reanimación en la pandemia por SARS-CoV-2. Una solución real coste-efectiva para una crisis sanitaria sin precedentes
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Villalonga Vadell, R.M., Sala Blanch, X., Roigé Solé, J., Garcia Eroles, X., Morros Viñoles, C., Valero Castell, R., Bausili Ribera, M., Pedregosa Sanz, A., Sergi Angeles Fite, G., Abreu Paradell, S., Adalia Bartolomé, R., Álvarez Pérez, A., Amigó Bonet, N., Asbert Sagasti, R., Barbal Badia, F., Carramiñana Dominguez, A., Castellnou Ferré, J., Bernia Gil, J.A., Carol Boeris, F., Colomina Soler, M.J., Chamero Pastilla, A., del Moral García, M.V., del Pozo, D., Escolano Villén, F., Esquius Jofre, P., Ferrando Ortolá, C., García Marqueta, C., Hernández Aguado, C., Hernando Pastor, D., Isach Comallonga, N., Isern Domingo, X., Cesar Jaramillo Carrión, L., Koo García, M., Lacambra Basil, M., Lázaro Alcay, J.J., Mailan Bello, J., Manrique Muñoz, S., Martínez Palli, G., Martínez Jérez, L., Masdeu Castellví, J., Mercadal Mercadal, J., Montero Matamala, A., Morales Torres, O., Moret Ferron, M.L., Moret Ruiz, E., Muñoz Falcón, L., de Nadal Clanchet, M., Miquel Ortells Nebot, P., Prat Vallribera, A., Remartinez Fernandez, F., Roca Campos, P., Roldan Osuna, J., Sabaté Pes, A., Sala Francino, P., Saludes Serra, J., Samsó Sabe, E., Sarmiento Meneses, E., Soto Ejarque, J.M., Tomas Torrelles, A., Tomás Esteban, A., and Yuste Graupera, M.
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- 2021
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171. Momento de inicio de radioterapia posoperatoria como factor pronóstico en pacientes con cáncer de laringe avanzado
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Daniel Muñoz S, José M Contreras R, Maritza Rahal E, and Pilar Contreras R
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Cáncer de laringe escamoso ,supervivencia ,radioterapia posoperatoria ,retraso ,General Medicine ,momento de inicio de radioterapia - Abstract
RESUMEN Introducción: Dentro de los factores que juegan un rol en la supervivencia y recidiva de enfermedad de los pacientes con cáncer laríngeo escamoso operados se encuentra el tiempo de inicio de la radioterapia (RT) posoperatoria. Objetivo: Determinar el impacto del retraso de inicio de RT posoperatoria en la supervivencia y recidiva de enfermedad en pacientes con cáncer de laringe escamoso avanzado operado. Material y método: Estudio tipo cohorte retrospectiva. Recolección de datos mediante revisión de fichas clínicas. Análisis de supervivencia y recidiva de enfermedad mediante el método de Kaplan-Meier, comparación de curvas con prueba de Log-Rank y modelo de regresión de Cox para análisis de factores pronósticos. Resultados: El tiempo de espera entre la cirugía y el inicio de la RT en nuestras realidades hospitalarias fueron 11 semanas. La supervivencia específica a 5 años en los pacientes que comienzan la RT ≤6 semanas desde la cirugía es de 33,3% y disminuye a 20% en aquellos que la comienzan >6 semanas (p =0,20). Conclusión: Los pacientes que inician la RT en más de 6 semanas desde la cirugía no presentan una diferencia estadísticamente significativa en el pronóstico.
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- 2018
172. Influencia del exceso de peso en el éxito de la adenoamigdalectomía en pacientes con hiperplasia adenoamigdalina sintomática
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Daniel Muñoz S, Leopoldo Córdova P, Gerardo Oberreuter E, Ignacio Cortés F, and Loreto Carrasco M
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adenoamigdalectomía ,sobrepeso ,hipertrofia ,adenoamigdalina ,General Medicine ,Hiperplasia ,obesidad - Abstract
Resumen Introducción: En población pediátrica con malnutrición por exceso, existe controversia respecto al rol de la hiperplasia adenoamigdalina en la etiología de los trastornos del sueño y de la efectividad de la adenoamigdalectomía sobre dicha sintomatología. Objetivo: Comparar la efectividad de la adenoamigdalectomía entre pacientes pediátricos eutróficos y con malnutrición por exceso sometidos a adenoamigdalectomía por hiperplasia adenoamigdalina, en relación a la disminución de la sintomatología. Materiales y método: Estudio retrospectivo mediante revisión de fichas clínicas entre junio de 2016 y enero de 2017 de pacientes operados de adenoamigdalectomía por hiperplasia adenoamigdalina sintomática. Se clasificaron los pacientes de acuerdo a edad y estado nutricional en 4 grupos y se evaluó la resolución de la sintomatologia mediante interrogación a padres/tutores. Resultados: Se incluyeron 98 pacientes, con una edad media de 6,3 años. 44,9% de los pacientes fueron eutróficos y 55,1% con malnutrición por exceso. El análisis estadístico entre pacientes eutróficos y aquellos con malnutrición no demostró diferencias significativas en relación a la resolución de la sintomatología. Conclusión: La adenoamigdalectomía por hiperplasia adenoamigdalina sintomática se asocia a una reducción de la frecuencia de roncopatía con pausas en pacientes pediátricos, independientemente del estado nutricional.
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- 2018
173. Application of multivariate latent variable modeling to pilot-scale spray drying monitoring and fault detection: Monitoring with fundamental knowledge
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Garcia-Munoz, S. and Settell, D.
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- 2009
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174. Adherencia al uso de audífonos en adultos mayores con hipoacusia: Un ensayo clínico aleatorizado para evaluar un programa de rehabilitación auditiva
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Cardemil M.,Felipe, Aguayo G.,Lorena, Fuentes L.,Eduardo, Muñoz S.,Daniel, Barría E.,Tamara, Fuente C.,Adrian, Rahal E.,Maritza, Yueh,Bevan, and Rojas C.,Graciela
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rehabilitación ,adulto mayor ,audífono ,adherencia ,Hipoacusia - Abstract
Resumen Introducción: La presbiacusia es una condición muy prevalente. Habitualmente se implementan audífonos como tratamiento, pero un problema es la baja adherencia exhibida que puede rondar un 50%. Objetivo: Evaluar si el programa de rehabilitación auditiva Active Communication Education (ACE) mejora la adherencia al uso del audífono. Material y Método: Se realizó un ensayo clínico aleatorizado controlado para evaluar la efectividad del programa ACE sumado a un refuerzo teleeducativo para mejorar la adherencia al uso del audífono. El proyecto fue aprobado por comité de ética y cada participante firmó consentimiento informado. La adherencia se evaluó por medio de la escala CIRUA y Dr. Yueh. Cada participante fue seguido en su domicilio en controles cada 3 meses hasta 1 año posterior a la intervención por encuestadores entrenados. Resultados: Se incluyeron 202 participantes, 101 en cada rama. La mediana de edad fue de 78 años y el 59,9% de la muestra fue de sexo femenino. El PTP promedio óseo fue de 48,59 dB y el aéreo fue de 55 dB. El grupo control tuvo adherencia de 65,91% y 62,30%. El grupo intervencional tuvo una adherencia de 78,41% y 72,28%. Hubo diferencias significativas en el porcentaje de adherencia entre grupo control y experimental según CIRUA (p = 0,027) y Yueh (p = 0,011). Conclusión: El programa de rehabilitación auditiva ACE es eficaz para mejorar la adherencia al uso del audífono en pacientes adultos mayores con hipoacusia. Este estudio representa uno de los mayores esfuerzos publicados para evaluar la efectividad de los programas de rehabilitación auditiva en adultos mayores con hipoacusia.
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- 2021
175. Parametric investigation of temperature distribution in field activated sintering apparatus
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Muñoz, S. and Anselmi-Tamburini, U.
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- 2013
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176. Arsenic, diet and occupational exposure on prostate cancer risk in Córdoba, Argentina
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Román, M, primary, Muñoz, S, additional, Niclis, C, additional, and Díaz, M, additional
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- 2014
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177. Thermal Conductivity of Powder Aggregates and Porous Compacts
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Montes, J. M., Cuevas, F. G., Cintas, J., and Muñoz, S.
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- 2012
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178. Anatomy and morphology of female flowers of ‘Serr’ walnuts (Juglans regiaL.), with and without pistillate flower abortion (PFA)
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González R., C., primary, Lemus S., G., additional, and Muñoz S., C., additional
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- 2021
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179. Enfermedad de Fabry y embarazo. Situación actual y revisión de la evidencia
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Blasco, F., primary, Guillamón, A.E., additional, and Muñoz, S., additional
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- 2021
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180. P-164 MONEO: A phase II study of avelumab plus FLOT in the peri-operative treatment for patients with resectable gastric or gastroesophageal junction cancer
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Alsina, M., primary, Ponz-Sarvise, M., additional, Lopez Garcia, D., additional, Gonzalez, M., additional, De Andrea, C., additional, Gros, A., additional, Vivancos, A., additional, Jimenez Fonseca, P., additional, Diez, M., additional, Arrazubi, V., additional, Sanz-García, E., additional, Martinez de Castro, E., additional, Guardeño Sanchez, R., additional, Calvo Campos, M., additional, Bugés Sánchez, C., additional, Muñoz, F., additional, Tabernero, J., additional, Villacampa, G., additional, Muñoz, S., additional, and Melero, I., additional
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- 2021
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181. Acute intrapapillary and peripapillary subretinal hemorrhage
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Vigués-Jorba, L., primary, Grau Verger, J., additional, Martin Pujol, R., additional, and Muñoz, S., additional
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- 2021
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182. Alcohol, methylxanthine-containing beverages, and colorectal cancer in Córdoba, Argentina
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Muñoz, S E, Navarro, A, Lantieri, M J, Fabro, M E, Peyrano, M G, Ferraroni, M, Decarli, A, La Vecchia, C, and Eynard, A R
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- 1998
183. N-nitroso compounds and man: sources of exposure, endogenous formation and occurrence in body fluids
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Hecht, S S, Muñoz, S E, Chatenoud, L, La Vecchia, C, Negri, E, Levi, F, and Hoffmann, D
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- 1998
184. Trends in cancer mortality in Argentina, 1966-91
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Muñoz, S E, Chatenoud, L, La Vecchia, C, Negri, E, and Levi, F
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- 1998
185. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, MA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Álvarez, ÉMC, Alves, S, Alves da Costa, C, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, Z, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, WL, Araya, 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Tuxworth, RI, Tyler, JK, Tyutereva, EV, Uchiyama, Y, Ugun-Klusek, A, Uhlig, HH, Ułamek-Kozioł, M, Ulasov, IV, Umekawa, M, Ungermann, C, Unno, R, Urbe, S, Uribe-Carretero, E, Üstün, S, Uversky, VN, Vaccari, T, Vaccaro, MI, Vahsen, BF, Vakifahmetoglu-Norberg, H, Valdor, R, Valente, MJ, Valko, A, Vallee, RB, Valverde, AM, Van den Berghe, G, van der Veen, S, Van Kaer, L, van Loosdregt, J, van Wijk, SJL, Vandenberghe, W, Vanhorebeek, I, Vannier-Santos, MA, Vannini, N, Vanrell, MC, Vantaggiato, C, Varano, G, Varela-Nieto, I, Varga, M, Vasconcelos, MH, Vats, S, Vavvas, DG, Vega-Naredo, I, Vega-Rubin-de-Celis, S, Velasco, G, Velázquez, AP, Vellai, T, Vellenga, E, Velotti, F, Verdier, M, Verginis, P, Vergne, I, Verkade, P, Verma, M, Verstreken, P, Vervliet, T, Vervoorts, J, Vessoni, AT, Victor, VM, Vidal, M, Vidoni, C, Vieira, OV, Vierstra, RD, Viganó, S, Vihinen, H, Vijayan, V, Vila, M, Vilar, M, Villalba, JM, Villalobo, A, Villarejo-Zori, B, Villarroya, F, Villarroya, J, Vincent, O, Vindis, 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Woehlbier, U, Wollert, T, Wong, E, Wong, JH, Wong, RW, Wong, VKW, Wong, WW-L, Wu, A-G, Wu, C, Wu, J, Wu, KK, Wu, M, Wu, S-Y, Wu, S, Wu, WKK, Wu, X, Wu, Y-W, Wu, Y, Xavier, RJ, Xia, H, Xia, L, Xia, Z, Xiang, G, Xiang, J, Xiang, M, Xiang, W, Xiao, B, Xiao, G, Xiao, H, Xiao, H-T, Xiao, J, Xiao, L, Xiao, S, Xiao, Y, Xie, B, Xie, C-M, Xie, M, Xie, Y, Xie, Z, Xilouri, M, Xu, C, Xu, E, Xu, H, Xu, J, Xu, L, Xu, WW, Xu, X, Xue, Y, Yakhine-Diop, SMS, Yamaguchi, M, Yamaguchi, O, Yamamoto, A, Yamashina, S, Yan, S, Yan, S-J, Yan, Z, Yanagi, Y, Yang, C, Yang, D-S, Yang, H, Yang, H-T, Yang, J-M, Yang, J, Yang, L, Yang, M, Yang, P-M, Yang, Q, Yang, S, Yang, S-F, Yang, W, Yang, WY, Yang, X, Yang, Y, Yao, H, Yao, S, Yao, X, Yao, Y-G, Yao, Y-M, Yasui, T, Yazdankhah, M, Yen, PM, Yi, C, Yin, X-M, Yin, Y, Yin, Z, Ying, M, Ying, Z, Yip, CK, Yiu, SPT, Yoo, YH, Yoshida, K, Yoshii, SR, Yoshimori, T, Yousefi, B, Yu, B, Yu, H, Yu, J, Yu, L, Yu, M-L, Yu, S-W, Yu, VC, Yu, WH, Yu, Z, Yuan, J, Yuan, L-Q, Yuan, S, Yuan, S-SF, Yuan, Y, Yuan, Z, Yue, J, Yue, Z, Yun, J, Yung, RL, Zacks, DN, Zaffagnini, G, Zambelli, VO, Zanella, I, Zang, QS, Zanivan, S, Zappavigna, S, Zaragoza, P, Zarbalis, KS, Zarebkohan, A, Zarrouk, A, Zeitlin, SO, Zeng, J, Zeng, J-D, Žerovnik, E, Zhan, L, Zhang, B, Zhang, DD, Zhang, H, Zhang, H-L, Zhang, J, Zhang, J-P, Zhang, KYB, Zhang, LW, Zhang, L, Zhang, M, Zhang, P, Zhang, S, Zhang, W, Zhang, X, Zhang, X-W, Zhang, XD, Zhang, Y, Zhang, Y-D, Zhang, Y-Y, Zhang, Z, Zhao, H, Zhao, L, Zhao, S, Zhao, T, Zhao, X-F, Zhao, Y, Zheng, G, Zheng, K, Zheng, L, Zheng, S, Zheng, X-L, Zheng, Y, Zheng, Z-G, Zhivotovsky, B, Zhong, Q, Zhou, A, Zhou, B, Zhou, C, Zhou, G, Zhou, H, Zhou, J, Zhou, K, Zhou, R, Zhou, X-J, Zhou, Y, Zhou, Z-Y, Zhou, Z, Zhu, B, Zhu, C, Zhu, G-Q, Zhu, H, Zhu, W-G, Zhu, Y, Zhuang, H, Zhuang, X, Zientara-Rytter, K, Zimmermann, CM, Ziviani, E, Zoladek, T, Zong, W-X, Zorov, DB, Zorzano, A, Zou, W, Zou, Z, Zuryn, S, Zwerschke, W, Brand-Saberi, B, Dong, XC, Kenchappa, CS, Lin, Y, Oshima, S, Rong, Y, Sluimer, JC, Stallings, CL, and Tong, C-K
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
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- 2021
186. Análisis transcultural de los niveles de actividad física y la intención de ser físicamente activo en población adolescente de Chile y España en función de variables sociodemográficas
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Rodríguez-Muñoz, S., Gallardo, L.O., Abarca Sos, A., and Moreno Doña, A.
- Abstract
El principal objetivo fue determinar si existen diferencias en los niveles de actividad física (NAF) y la intención de ser físicamente activo entre países en función del género, curso y estatus socioeconómico. Participaron 3052 adolescentes (14.58 ± 1.39 años), 1516 chicos y 1536 chicas. Del total, 734 chilenos y 2318 españoles. Las variables medidas fueron los NAF con el “International Physical Activity Questionnaire- Short Form” (IPAQ-SF), la intención de ser físicamente activo con el “Intention to be Physically Active” y el estatus socioeconómico familiar con el “Family Affluence Scale II” (FASII). Se encontraron diferencias significativas en los NAF y en la intención de ser físicamente activo entre países en función del género (p < .001), curso (p < .001) y estatus socioeconómico (p < .01), siendo más altas en España. Además, la relación de cada una de estas variables con los NAF y la intencionalidad varía según el país. Como la relación de las variables sociodemográficas con los NAF y la intención de ser físicamente activo varía en función del país sería necesario llevar a cabo más estudios transculturales que permitan realizar intervenciones de promoción de actividad física y salud adaptadas a las necesidades de cada lugar. The main goal was to analyze the physical activity levels and the intention to be physically active differences between both countries regarding the gender, course and socioeconomic status.The sample was 3,052 adolescents (14.58 ± 1.39 years), 1,516 males and 1,536 females. From the whole participants, 734 were Chilean and 2,318 Spanish.Physical activity levels were measured using the “International Physical Activity Questionnaire- Short Form” (IPAQ-SF), the intention to be physically active using the “Intention to be Physically Active” scale and the socioeconomic status using the “Family Affluence Scale II” (FASII). There are significant differences in physical activity levels and the intention to be physically active between both countries according to the gender (p < .001), course (p < .001) and socioeconomic status (p < .01). Data were higher in Spain than Chile. Moreover, the relation between these sociodemographic variables with physical activity levels and the intention to be physically active were different regarding the country. As the relation between these sociodemographic variables with physical activity levels and the intention to be physically active is different according to the country, cross-cultural researches are required to develop physical activity and health interventions adapted to people`s needs.
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- 2021
187. Absorción sistémica de un jarabe de vancomicina oral para el tratamiento de la infección por Clostridium difficile
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Edo Peñarrocha,J, Belles Medall,MD, Garcia Muñoz,S, and Ferrando Piqueres,R
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Vancomicina oral ,absorción sistémica ,Clostridium difficile - Abstract
RESUMEN La infección por Clostridium difficile (ICD) ha aumentado su incidencia en los últimos años, convirtiéndose en una de las infecciones más comunes adquiridas en el ámbito hospitalario, causando una diarrea infecciosa que está fuertemente asociada al uso de antibióticos. En la actualidad, los antibióticos para su tratamiento son la vancomicina oral y fidaxomicina, siendo la vancomicina oral la más coste-efectiva para el Sistema Nacional de Salud. Se describe el caso de una paciente pluripatológica sometida a dos sesiones de hemodiálisis semanales, que es diagnosticada de ICD evolucionada a colitis pseudomembranosa confirmada por endoscopía. Se inició tratamiento para la ICD con una fórmula magistral de vancomicina oral a una dosis de 250 mg cada 6 horas. La terapia con vancomicina oral no fue efectiva ni segura en la paciente, produciéndose una absorción sistémica significativa de vancomicina llegando a concentraciones plasmáticas de 15,57 mcg/mL. Fueron necesarios 8 días para completar la eliminación de la vancomicina, durante los cuales la paciente se sometió a 4 sesiones de hemodiálisis. El caso descrito pone de manifiesto la necesidad de monitorizar las concentraciones plasmáticas de vancomicina durante el tratamiento de ICD, especialmente en pacientes con elevadas dosis de fármaco, con insuficiencia renal y afectación de la integridad del tracto gastrointestinal.
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- 2021
188. High-dose versus low-dose corticosteroid treatment strategy in patients hospitalised with COVID-19: effect on ICU admission rate
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García Muñoz,S, Santos San Segundo,M, Cebolla Beltrán,T, Boso Ribelles,V, and Ferrando Piqueres,R
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SARS-Cov-2 ,ICU ,COVID-19 ,corticosteroids - Abstract
SUMMARY Objective: COVID-19 is associated with lung damage and a high mortality rate in hospitalised patients. Corticoids may reduce the evolution to respiratory failure and death. This study aims to assess the effect on the ICU admission rate of a change in corticosteroid treatment strategy, administering low versus high doses. Methods: A retrospective, observational study was designed. Confirmed COVID-19 patients indicated to start treatment with corticosteroids were enrolled. To study the association between the type of corticosteroid used and admission to the ICU, a binary logistic regression model was constructed. This model included variables that could cause confusion or influence the response: gender, age, comorbidities, and analytical data. Results: From 190 admitted patients, 127 were enrolled in the study. In both groups, patients received a minimum of two doses of corticosteroids during admission. 12.4% (12/97) of the patients who received methylprednisolone (high doses) were subsequently admitted to the ICU, compared to 30.0% (9/30) of the patients who received dexamethasone (low doses). In the logistic regression model constructed, the type of corticosteroid (low-dose dexamethasone) (p=0.002), male gender (p=0.023), age over 50 years (p=0.014) and IL-6 level >70 pg/mL (p=0.004) remained as predictive factors for admission to the ICU. Conclusions: In the studied population of patients hospitalised for COVID-19, the use of high-dose methylprednisolone is associated with a lower rate of admission to the ICU than the administration of low-dose dexamethasone.
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- 2021
189. SARS-CoV-2 Antibody Prevalence Among 85,529 Healthcare Workers Following the First Wave of COVID-19 in Chile
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Alonso Ap, Bravo P, Anne J. Lagomarcino, Pablo Vial, Muñoz S, Zuñiga M, María Paz Bertoglia, Rodriguez Ma, and M O'Ryan
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Emergency rooms ,Increased risk ,Venipuncture ,Coronavirus disease 2019 (COVID-19) ,Fingerstick ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Health care ,Medicine ,business ,Antibody prevalence ,Demography - Abstract
Background: Healthcare workers (HCWs) are at increased risk for SARS-CoV-2 infection, however not all face the same risk. We aimed to determine antibody prevalence and risk factors associated with seropositivity in the Chilean HCW community. Methods: This was a nationwide, cross-sectional study consisting of a questionnaire and COVID-19 antibody testing. All HCWs in the Chilean public health care system were invited to participate three to four months following the peak of the country's first wave. Findings: Overall SARS-Cov-2 blood antibody positivity by fingerstick or venipuncture in 85 529 HCWs was 7 · 2%, ranging from 1 · 6% to 12 · 4% between regions. SARS-Cov-2 positive PCR results were self-reported in 8 330 individuals (9 · 7%) of which 47% were seropositive. Overall 10 863 (12 · 7%) either reported prior PCR positive results and/or were seropositive. Several factors were independently associated with higher IRR for seropositivity, including working in hospital (IRR 1·484), medicine/surgery w ards (IRR 1·383), emergency room (IRR 1·266), and night shifts (IRR 1·616), as were history of contact with a confirmed case (IRR 1·462), and use of public transport (IRR 1·367). These variables remained significant when including self-reported PCR positive cases in the model. Interpretation: HCWs in the hospital were at highest risk for COVID-19, especially if working in medicine/surgery wards or emergency rooms, in night shifts, older age, exposed to confirmed cases and/or using public transport. Antibody results using lateral flow likely underestimated true infection rates by nearly 40-50%. Nevertheless, risk factors were sustained when adjusting for self-reported PCR positive cases. Funding: No funding institution. Conflict of Interest: Authors do not declare any conflict of interest. Ethical Approval: The study was designed in August 2020 by Health Ministry personnel in conjunction with an academicadvisory board; the study was approved by the Ethical Committee of the Servicio de Salud Araucania Sur (No CEC-201, August 10, 2021).
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- 2021
190. Estimation of the through-plane thermal conductivity of polymeric ion-exchange membranes using finite element technique
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Barragán García, Vicenta María, Izquierdo Gil, María Amparo, Maroto, J. C., Antoranz Canales, Pedro, Muñoz, S., Barragán García, Vicenta María, Izquierdo Gil, María Amparo, Maroto, J. C., Antoranz Canales, Pedro, and Muñoz, S.
- Abstract
CRUE-CSIC (Acuerdos Transformativos 2021) Financial support of this work by Banco de Santander and Universidad Complutense de Madrid within the framework of Projects: PR75/18-21589, PR108/20-02 andthe Project for Research Groups (Bioelectromagnetism Research Group 910305) are gratefully acknowledged., The aim of this study is to calculate the through-plane thermal conductivity of commercial polymeric ion-exchange membranes. Different membranes were considered to study the influence of membrane properties on the thermal conductivity values. In particular we focused on reinforcement, ion exchange capacity and membrane density and thickness. For this purpose, we use a simple experimental setup and a numerical simulation to estimate the thermal conductivity from the experimental temperature profiles. Once the system is calibrated, the model includes as the only unknown parameter the membrane thermal conductivity. To validate the method, the thermal conductivity of the well-known Nafion membranes has been determined, a very good agreement was achieved in context from reliable literature values. The study also provides the thermal conductivity of other polymeric ion-exchange membranes with great potential in diverse applications under non-isothermal conditions. The calculated thermal conductivity for the different ion-exchange membranes is in the range from 0.04 Wm(-1) K-1 to 0.42 Wm(-1) K-1. The results show that the reinforcement leads to lower values of thermal conductivity whereas a higher density or heterogenous structure leads to higher thermal conductivity values. The approach presented here, combining experimental and simulation techniques, may provide a basis for confirming the effect of the polymeric ion-exchange membrane properties on the thermal conductivity and may shed light on the best choice for the electrolyte of membrane-based applications performance under non-isothermal conditions. Published by Elsevier Ltd., Universidad Complutense de Madrid/Banco de Santander, Universidad Complutense de Madrid, Depto. de Estructura de la Materia, Física Térmica y Electrónica, Fac. de Ciencias Físicas, TRUE, pub
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- 2021
191. Multiplex RNA-based detection of clinically relevant MET alterations in advanced non-small cell lung cancer
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Aguado, C., Teixido, C., Román, R., Reyes, R., Giménez-Capitán, A., Marin, E., Cabrera, C., Viñolas, N., Castillo, S., Muñoz, S., Arcocha, A., López-Vilaró, L., Sullivan, I., Aldeguer, E., Rodríguez, S., Moya, I., Viteri, S., Cardona, A.F., Palmero, R., Sainz, C., Mesa-Guzmán, M., Lozano, M.D., Aguilar-Hernández, A., Martínez-Bueno, A., González-Cao, M., Gonzalvo, E., Leenders, W.P.J., Rosell, R., Montuenga, L.M., Prat, A., Molina-Vila, M.A., Reguart, N., Aguado, C., Teixido, C., Román, R., Reyes, R., Giménez-Capitán, A., Marin, E., Cabrera, C., Viñolas, N., Castillo, S., Muñoz, S., Arcocha, A., López-Vilaró, L., Sullivan, I., Aldeguer, E., Rodríguez, S., Moya, I., Viteri, S., Cardona, A.F., Palmero, R., Sainz, C., Mesa-Guzmán, M., Lozano, M.D., Aguilar-Hernández, A., Martínez-Bueno, A., González-Cao, M., Gonzalvo, E., Leenders, W.P.J., Rosell, R., Montuenga, L.M., Prat, A., Molina-Vila, M.A., and Reguart, N.
- Abstract
Contains fulltext : 235656.pdf (Publisher’s version ) (Open Access), MET inhibitors have shown activity in non-small-cell lung cancer patients (NSCLC) with MET amplification and exon 14 skipping (METΔex14). However, patient stratification is imperfect, and thus, response rates have varied widely. Here, we studied MET alterations in 474 advanced NSCLC patients by nCounter, an RNA-based technique, together with next-generation sequencing (NGS), fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and reverse transcriptase polymerase chain reaction (RT-PCR), exploring correlation with clinical benefit. Of the 474 samples analyzed, 422 (89%) yielded valid results by nCounter, which identified 13 patients (3%) with METΔex14 and 15 patients (3.5%) with very-high MET mRNA expression. These two subgroups were mutually exclusive, displayed distinct phenotypes and did not generally coexist with other drivers. For METΔex14, 3/8 (37.5%) samples positive by nCounter tested negative by NGS. Regarding patients with very-high MET mRNA, 92% had MET amplification by FISH and/or NGS. However, FISH failed to identify three patients (30%) with very-high MET RNA expression, among which one received MET tyrosine kinase inhibitor treatment deriving clinical benefit. Our results indicate that quantitative mRNA-based techniques can improve the selection of patients for MET-targeted therapies.
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- 2021
192. Comparación de sutura mecánica con sutura manual de la faringe en laringectomía total
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Osorio M.,Jaime, Morales A.,Carlos, Contreras R.,Pilar, Pineda D.,Rodrigo, Silva R.,Fernanda, Muñoz S.,Daniel, Suazo D.,Angélica, Cancino C.,Miguel, Arregui V.,Rodrigo, Osorio M.,Jaime, Morales A.,Carlos, Contreras R.,Pilar, Pineda D.,Rodrigo, Silva R.,Fernanda, Muñoz S.,Daniel, Suazo D.,Angélica, Cancino C.,Miguel, and Arregui V.,Rodrigo
- Abstract
Resumen Introducción: La sutura mecánica es una opción para el cierre de la faringorrafia en laringectomía total por cáncer de laringe. Objetivo: Comparar el uso de sutura mecánica lineal con sutura manual de la faringe durante la laringectomía total por cáncer de laringe. Material y Método: Se realizó un estudio de tipo experimental prospectivo. Se compararon dos grupos pacientes: Los pacientes con sutura mecánica desde 2018 a marzo de 2020 y los pacientes con sutura manual previa a enero de 2018 en el Servicio de Otorrinolaringología del Hospital Barros Luco. Se analizaron fístula faringo-cutánea posoperatoria (FFC), tiempo operatorio, estadía hospitalaria y costo. Resultados: El estudio incluyó a 14 pacientes, cada grupo con n = 7. El grupo con sutura mecánica presento 0% de FFC y el grupo sutura manual 28%. El grupo con sutura mecánica reinicio alimentación a los 7 días y el grupo con sutura manual en promedio a los 11,5 (7-23) días. (p = 0,0023). El tiempo promedio de cirugía para el grupo experimental es de 288 ± 37,4 minutos y con sutura manual 311 ± 32,4 minutos. (p = 0,0176). El promedio de hospitalización para el grupo experimental fue de 11 ± 2,6 días (9 a 16), y para el grupo control fue de 21 ± 14 días (10 a 49) (p < 0,0001). Conclusión: La sutura mecánica es un procedimiento fácil de usar y seguro. Existiría un beneficio en el uso de sutura mecánica para el cierre faríngeo al compararlo con la sutura manual al disminuir el número de FFC, el tiempo operatorio y los días de hospitalización.
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- 2021
193. Validation of analytical method by UV spectrophotometric quantification of gemfibrozil incorporated in the microemulsions
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Herrera P., María, Cantos C., José, Muñoz S., Kevin, Durán A., Jovan, Vinueza G., Julio, Kolenyak dos Santos, Fernanda, Herrera P., María, Cantos C., José, Muñoz S., Kevin, Durán A., Jovan, Vinueza G., Julio, and Kolenyak dos Santos, Fernanda
- Abstract
The present study aims to validate an analytical method by UV spectrophoto-metric for the quantification of gemfibrozil incorporated into microemulsions.Experimental development by constructing a ternary phase diagram for the iden-tification of microemulsions. The characterization of the microemulsions was carried out by the polarized light microscopy technique. The analytical methodwas validated according to the parameters described by the United State Phar-macopeia (USP) 39 NF34 and International Conference on Harmonization(ICH) (2005), which evaluated linearity, robustness, intra and inter day accuracy,specificity accuracy, limits of detection and quantification. The developed met-hod demons trated linearity with a coefficient of determination (R2) of 0.9898,in addition to precision, accuracy and robustness for each of the tests with va-lues that presented a coefficient of variation of less than 5 %. The results obtai-ned were favorable, since they comply with the parameters stipulated by USP39-NF34 (2016) and ICH (2005), indicating that the method is effective for theintended purpose.
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- 2021
194. Information retrieval on documents methodology based on entropy filtering methodologies
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Universidad EAFIT. Escuela de Ciencias, Modelado Matemático, Montoya, O.L.Q., Villa, L.F., Muñoz, S., Arenas, A.C.R., Bastidas, M., Universidad EAFIT. Escuela de Ciencias, Modelado Matemático, Montoya, O.L.Q., Villa, L.F., Muñoz, S., Arenas, A.C.R., and Bastidas, M.
- Abstract
Information retrieval problem occurs when the target information is not available 'literally' into the set of documents. In problems in which the goal is to find 'hidden' information, it is important to develop hybrid methodologies or improve and design a new one. In this work the authors are dealing with identifying the most informative piece of data on a collection of documents, in order to obtain the best result on a posterior fuzzy clustering stage. The aim is to find similarities between the documents and a reference target, to establish relationships related to a non-literal feature. We propose to apply the well-known entropy term weighting scheme and then show a posterior different procedures to the right election of the interest data. This procedure brings the biggest amount of information within the smallest amount of data. Applying a specific selection procedure for a group of words, gives more information to differentiate and separate the documents after using the entropy weighting. This returns considerable results on the processing time and the right fuzzy clustering of the documents collection. Copyright © 2015 Inderscience Enterprises Ltd.
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- 2021
195. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, D, Abdel-Aziz, A, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, M, Abudu, Y, Acevedo-Arozena, A, Adamopoulos, I, Adeli, K, Adolph, T, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, B, Agnello, M, Agostinis, P, Agrewala, J, Agrotis, A, Aguilar, P, Ahmad, S, Ahmed, Z, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, H, Al-Abd, A, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, M, Alberti, S, Alcocer-Gómez, E, Alessandri, C, Ali, M, Alim Al-Bari, M, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, G, Altan-Bonnet, N, Altieri, D, Álvarez, É, Alves, S, Alves da Costa, C, Alzaharna, M, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, A, Ammanathan, V, An, Z, Andersen, S, Andrabi, S, Andrade-Silva, M, Andres, A, Angelini, S, Ann, D, Anozie, U, Ansari, M, Antas, P, Antebi, A, Antón, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araújo, W, Araya, J, Arden, C, Arévalo, M, 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Werner, Brand-Saberi, Beate, Dong, X Charlie, Kenchappa, Chandra Shekar, Li, Zuguo, Lin, Yong, Oshima, Shigeru, Rong, Yueguang, Sluimer, Judith C, Stallings, Christina L, and Tong, Chun-Kit
- Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
196. 1278P ORIGEN: Multicenter study on the prevalence of EGFR gene mutations in patients with early-stage resectable non-small cell lung cancer in Spain
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Nadal, E., Fernandez, C. Alvarez, Arasanz, H., Muñoz, S. Peralta, Quintela, M. Lázaro, Teixido, C., Calvo de Juan, V., Alvarez, R.M., Espinar, J. Baena, Valdivia-Bautista, J., Arriola, E., Lastra, R., Caro, R. Bernabe, Camacho, C., Cordellat, A. Blasco, Sureda, B. Massuti, Guillaumes, M. Campayo, Dols, M. Cobo, Manrique, T. Garcia, and Marquez, G.J.
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- 2023
- Full Text
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197. LBA28 The PEGASUS trial: Post-surgical liquid biopsy-guided treatment of stage III and high-risk stage II colon cancer patients
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Lonardi, S., Pietrantonio, F., Tarazona Llavero, N., Montagut Viladot, C., Sartore Bianchi, A., Zampino, M.G., Elez Fernandez, M.E., Santos Vivas, C., Mandalà, M., Tamberi, S., Sciallero, M.S., Muñoz, S., Lazzari, L., Luraghi, P., Torri, V., Cervantes, A., Bardelli, A., Tabernero, J., Siena, S., and Marsoni, S.
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- 2023
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198. Nuevas aproximaciones al arte rupestre de Fuego-Patagonia, Chile: Caracterización y comparación de los sitios del continente y de los canales del extremo sur
- Author
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Camila Muñoz S.
- Subjects
Patagonia continental ,intercambio de información ,General Social Sciences ,códigos visuales ,arte rupestre ,canales - Abstract
Resumen: En este trabajo se presentan los resultados obtenidos a partir del registro de siete sitios con arte rupestre de Patagonia meridional, Chile: cuatro en el sector del continente y tres en los canales, a los cuales se integraron los datos de 44 yacimientos publicados en diferentes fuentes bibliográficas. A partir del análisis cuantitativo y estadístico realizado a través de tres unidades diferentes compuestas por el motivo (micro), el conjunto o panel (meso) y el sitio (macro), se busca ahondar, por primera vez, en la comparación de los contextos de los canales y el continente de la región de Magallanes, para dar luces respecto al posible intercambio de información y códigos visuales entre los distintos grupos que habitaron dicha región.
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- 2020
199. In vivo genetic engineering of murine pancreatic beta cells mediated by single-stranded adeno-associated viral vectors of serotypes 6, 8 and 9
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Jimenez, V., Ayuso, E., Mallol, C., Agudo, J., Casellas, A., Obach, M., Muñoz, S., Salavert, A., and Bosch, F.
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- 2011
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200. Punción ecodirigida de la vena yugular interna por abordaje posterior
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Pérez Reyes, J.M., Bethencourt Muñoz, S., Cabrejas Ibarz, M.T., Tejero García, M., Valdivia Martín, J., and González Miranda, F.
- Published
- 2008
- Full Text
- View/download PDF
Catalog
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