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Objective. Submaximal myocardial hyperemia is known not to be achieved in 16-18% of cases during cardiac stress tests and monotonic intracubital administration of adenosine triphosphate (ATP) at a dose of 140-160 pg/kg/min. The authors set a task to elaborate a new algorithm for stress echocardiography (SEchoCG) with ATP, providing for the possibility of a stepwise increase in the dosage of the drug, as well as to test it in healthy individuals and patients with coronary heart disease (CHD).Material and methods. The authors elaborated a new algorithm for SEchoCG on the basis of an analysis of the main science databases and their first own experience in using ATP during SEchoCG. The key provisions of the new algorithm were: (a) the exercise test consisted of 3 stages (EchoCG data should be recorded before, during, and 5 minutes after ATP infusion); (b) the criterion for achieving submaximal myocardial hyperemia during ATP administration is a systolic blood pressure (SBP) reduction of 5 and more mm Hg; (c) EchoCG was usually recorded at Stage 2 of the test 3 minutes after the start of ATP administration and with a decline in SBP; (d) the initial dose of ATP administration was 140 pg/kg/min; if SBP did not decrease at 3 minutes of the drug administration, the dosage should be first increased up to 175 pg/kg/ min at 1 minute; if there was no effect, the dosage should be increased up to 210 pg/kg/min at another 2-3 minutes. The algorithm was tested in 9 healthy volunteers, and in 26 patients with CHD.Results. Testing the new algorithm showed that all cases achieved submaximal myocardial hyperemia. SBP decreased below 90 mm Hg in 2 patients; 1 patient developed second-degree atrioventricular block; however, a simple decrease in the ATP infusion rate within 30 sec leveled this symptomatology. The SEchoCG data acceptable for the subsequent analysis of myocardial contractility and deformation could be recorded in all the examinees.Conclusion. The new algorithm for SEchoCG with ATP is effective in recording EchoCG findings. To have a final decision on the safety and information value of a new stress test protocol, it may be recommended to further test those in larger groups of patients during SEchoCG and in the use of other imaging procedures to assess myocardial contractility and perfusion.

Specialists in pharmacognosy are facing an important task of improving quality control methods for combination herbal medicinal products in order to enable reliable assessment of the content of active substances that are responsible for the drug’s pharmacological effect.The aim of the study was to investigate the possibility of using the European Pharmacopoeia method to determine the total content of anthracene derivatives in the antihaemorrhoidal medicinal herb mixture Proctophytol® and to propose limit values for the total content of anthracene derivatives.Materials and methods: individual mixture components containing anthracene derivatives, such as senna leaves and frangula bark, as well as model mixtures containing these individual components in a 1:1 ratio, and a model mixture imitating Proctophytol® were used in the study. The determination of the anthracene derivatives content was carried out using spectrophotometry.Results: it was demonstrated that the European Pharmacopoeia method could be used to assess the quality of Proctophytol® in terms of anthracene derivatives total content. The spectrophotometric method described in the European Pharmacopoeia has advantages over the methods described in manufacturer specifications for Russian products, because it allows for thorough extraction of the active substances and is standardized for anthracene derivatives.Conclusions: the European Pharmacopoeia method was adjusted to determine anthracene derivatives in the medicinal herb mixture Proctophytol®. It was proposed to use 70% ethanol instead of 70% methanol as extraction solvent. The authors identified optimum sample weights and test solution dilutions, and calculated and verified the limit for anthracene derivatives content—“Not less than 1.9%”. The adjusted method can be recommended for inclusion in the monographs of the State Pharmacopoeia of the Russian Federation for medicinal herb mixtures similar to Proctophytol®.

Alogliptin, a dipeptidylpeptidase-4 inhibitor, is an oral hypoglycemic agent approved in many countries for the treatment of patients with type 2 diabetes, including the United States, Europe, and Japan. The drug is effective both as a monotherapy, and as an additional or combined treatment of type 2 diabetes. Alogliptin is well tolerated by patients, including the elderly, as well as those suffering from kidney and / or liver failure or having a high risk of cardiovascular events. The low risk of hypoglycemia, weight gain, acute pancreatitis, and side gastrointestinal events. During treatment with alogliptin has been demonstrated in both long-term (up to 4.5 years) studies and in actual clinical practice. Alogliptin increases postprandial levels of the glucagon-like peptide-1, which leads to insulin secretion and normalization of glucose homeostasis. Treatment with alogliptin is associated not only with improved glucose metabolism, but also with a decrease in blood pressure and arterial rigidity in patients with arterial hypertension and diabetes, as well as normalizing the lipid profile. In patients with diabetes mellitus who have recently undergone acute coronary syndrome and received alogliptin, the frequency of serious adverse cardiovascular events does not increase. Experimental data show that alogliptin reduces ventricular hypertrophy, interstitial fibrosis, and diastolic dysfunction. Alogliptin has a number of unique properties. It is assumed that it can increase the number of circulating endothelial progenitor cells that play an important role in endothelial repair and neovascularization. Alogliptin preserves the functionality and structure of the mitochondria of cardiomyocytes. The drug may be a potential treatment for patients with MODY1 diabetes at an early stage of the disease, when residual insulin secretion is preserved. Treatment with a fixed combination of Alogliptin + Metformin results in better glycemic control than monotherapy and is well tolerated. There is evidence that treatment with alogliptin is not associated with an increased risk of pancreatitis or pancreatic cancer.

The article presents the results of a comparative study of the biological, molecular and genetic characteristics of African swine fever virus (ASFV) isolates, obtained from domestic pigs and wild boars in the Russian Federation and South Ossetia from 2016 to 2018. The studied isolates caused the death of pigs manifesting, as a rule, signs of an acute or subacute form of the disease when using various methods of infection including intramuscular, direct contact, intranasal and oral routes. The virus was hemadsorbing, belonging to serotype 8 and genotype II, and accumulated in the blood with a titer of 6.5 to 7.5 lg HAU50/cm3. The ASFV isolates circulating in the central region of Russia were found to have an insertion of 10 base pairs in the intergenic region I73R/I329L. However, the ASFV isolated in the Irkutsk region and South Ossetia, as well as Georgia 2007/1 (FR682468.1), lacked this insertion.

The article provides a sequence of steps for using RISØ calorimeters for calibration and subsequent use of B3 radiochromic film dosimeters (GEX corporation) and a scanner for measuring absorbed doses. Calibration was carried out with the help of electron beam accelerator in the range of absorbed doses of 3 – 40 kGy (measurement range of RISØ calorimeters). In the course of the work, the following was carried out: – calibration of B3 radiochromic dosimetry films using RISØ calorimeters; – plotting a calibration curve for B3 radiochromic dosimetric films; – calculation of approximation functions; – development of a technique for using a flatbed scanner to measure absorbed doses; – estimation of the measurement uncertainties of absorbed doses. Accelerator operation parameters: scanning frequency of the accelerated electron beam – 5 Hz, pulse frequency – 120 Hz, electron energy – 5 MeV, electron beam current – 60 μA. The measurement error of the absorbed dose is 5.8 %.

Isocitric acid (ICA) refers to a group of promising regulators of energy metabolism which has antistress, antihypoxic, and antioxidant activities. In this paper, we reported a process of ICA production from rapeseed oil using yeast Yarrowia lipolytica VKM Y-2373 in a 500-L fermentor. The producer synthesized 64.1 g/L ICA with a product yield of 0.72 g/g and a productivity 0.54 g/L·h. We also developed an effective purification method, including a cell separation, clarification, concentration, acidification, and crystallization process, which resulted in the formation of the crystals of monopotassium salt of ICA with a purity of 99.0–99.9%. To the best of our knowledge, this is the first report on an ICA production process at an upscaled bioreactor level.

African swine fever virus (ASFV) is an extremely genetically and phenotypically heterogeneous pathogen. Previously, we have demonstrated that experimental inoculation of pigs with an attenuated strain, Katanga-350 (genotype I, seroimmunotype I) (ASFV-Katanga-350), can induce protective immunity in 80% of European domestic pigs against the homologous virulent European strain Lisbon-57. At least 50% of the surviving pigs received protection from subsequent intramuscular infection with a heterologous virulent strain, Stavropol 01/08 (genotype II, seroimmunotype VIII) (ASFV-Stavropol 01/08). In this study, we assessed clinical signs, the levels of viremia, viral DNA, anti-ASFV antibodies and post-mortem changes caused by subsequent intramuscular injection with ASFV-Katanga-350 and heterologous ASFV-Stavropol 01/08. Inoculation of pigs with the ASFV-Katanga-350 did not protect animals from the disease in the case of the subsequent challenged ASFV-Stavropol 01/08. However, 40% of pigs were protected from death. Moreover, the surviving animals showed no pathomorphological changes or the presence of an infectious virus in the organs after euthanasia at 35 days post challenging. The ability/inability of attenuated strains to form a certain level of protection against heterologous isolates needs a theoretical background and experimental confirmation.

Bread and bakery products are one of the staple foods and make up a significant part of the daily human diet. However, the biological value is low and the content of essential amino acids is insufficient. In this regard, the goal of the work was to develop a recipe for wheat bread with the addition of amaranth flour and coriander. To determine the optimal recipe, laboratory baking of prototypes was carried out with different ratios of additional components. The addition of amaranth flour and ground coriander affected the color of the samples: from light yellow in the control to light brown in the test samples. The taste and smell of the prototypes also changed with the increase in non-traditional raw materials in the recipe. The acidity of the crumb increased depending on the amount of added amaranth flour and ground coriander: from 2.5 ºT for the control sample to 2.7 for the 3rd experimental sample. An inversely proportional effect of amaranth flour and ground coriander on porosity was established, which decreased from 75.0% for the control sample to 71.0% for test sample 3. The addition of amaranth flour and ground coriander to the recipe had a positive effect on the mass fraction of protein - the maximum value This indicator was recorded in samples 2 and 3 (8.3%), which is 0.8-0.1 abs. percent higher compared to the performance of the control sample and prototype 1

The temporality of the socio-technological transformations of industry 4.0 has exacerbated the problem of introducing and using digital technologies in the educational process. The development in this area is due to the presence of unique global challenges, the availability of digital devices, and the experience of distance learning due to the COVID-19 pandemic. As part of the work, a statistical study was conducted aimed at establishing the average level of proficiency in the basic skills of independent search for information on the Internet. A classification of digital solutions is formulated, and an attempt is made to synthesize criteria for the successful integration of digital solutions into the educational process, the following groups are distinguished: organizational, auxiliary, structural, and specialized. Independent searching for information is considered an element of the educational process, which changes almost uncontrollably, unlike other solutions in the field of digitalization of education. The process of digitalization is presented as a set of four elements, the successful implementation of which will allow the effective implementation of digital technologies in the educational process. An element of digital infrastructure is highlighted; an element of organization, an element of integrating teachers into a new digital educational environment, and a student element. It is concluded that it is necessary to introduce courses of additional competencies dedicated to working with information; the idea of integrating master’s students into research aimed at solving modern global challenges with the help of digital technologies is proposed.

The methodological and technical possibilities of monitoring temperature fields in the Sea of Japan by acoustic thermometry methods are presented. The proposed tomographic method for monitoring the dynamics and structure of water is based on the transmission and reception of complex phase-shift keyed acoustic signals on a diagnosed track with the determination of the travel time along various ray trajectories, followed by the sound speed (temperature) determination. The physical prerequisites for the practical implementation of thermometric studies at large distances are based on the acoustic “mudslide” effect—the phenomenon of the acoustic energy “injection“ from the near-bottom shelf area to the underwater sound channel of the deep ocean. Based on the Sea of Japan example, an acoustic thermometry system based on tomographic schemes with mobile and stationary hydroacoustic sources for promising work in the field of oceanic climatology is proposed. For numerical calculations of the signal propagation channels’ impulse responses between sources and receivers, a specialized database of oceanological information was formed for the northwestern part of the Sea of Japan. The database includes all available data from organizations in Russia, Japan, North Korea, the Republic of Korea, and the United States (23,247 stations completed from 1925 to 2017). In the example of the Sea of Japan, a high-precision acoustic thermometry system based on tomographic schemes with developed mobile and stationary hydroacoustic transmitting and receiving systems was proposed and experimentally tested.

Anomalous Hall effect (AHE) in GdFeCo/Ir/GdFeCo multilayered structures attracts great interest because all optical switching, spin-torque, and other effects promise effective application for ultrafast memory element creation. Since AHE is controlled by GdFeCo magnetization, domain dynamics has importance for practical applications. In our work, magnetization reversal in perpendicular GdFeCo/Ir/GdFeCo synthetic ferrimagnets is characterized by AHE measurements. The AHE hysteresis loop obtained with the field applied perpendicular to the sample plane is composed of three sub-loops, and two of them are symmetrically biased with respect to the third one. Switching magnetic fields for two of the three transitions are found to be dependent on magnetic history. In particular, exposure of the sample in the in-plane field leads to reduction of the out-of-plane switching fields in side sub-loops. A multiple series of perpendicular hysteresis loops recorded after exposure under high in-plane field reveals gradual (within 30 min) relaxation of the out-of-plane switching fields to their initial values observed in a non-magnetized sample. Domain wall mobility, limiting switching of the bilayer devices, is complicated due to the coupling between partial domains in each single layer. Unusual dynamics of double domain walls results in unexpected new phenomena affecting electrical processes in bilayer structures. [ABSTRACT FROM AUTHOR]

The series of Co(II), Fe(II), and Ni(II) mononuclear coordination compounds of [CoL(NCS)2]·3DMSO (1), [CoL(H2O)2](ClO4)2·DMSO (2), [CoL(H2O)(EtOH)][CoCl4]·2H2O (2a), [FeL(NCS)2]·DMSO (3), and [NiL(NCS)2]·CH3CN (4) composition (where L is 2,6-bis(1-(2-(4,6-dimethylpyrimidin-2-yl)hydrazineylidene)ethyl)pyridine), with an [MLA2] coordination unit (where A is a pair of apical monodentate ligands), was synthesized. In compounds 1, 2, 2a, and 3, the ligand L is pentadentate, and cobalt and iron ions are placed in a heavily distorted pentagonal pyramidal coordination environment, while in 4 the Ni(II) ion is hexacoordinated. Easy plane-type magnetic anisotropy (D = 13.69, 11.46, 19.5, and 6.2 cm−1 for 1, 2, 2a, and 4, respectively) was established for cobalt and nickel compounds, while easy axis-type magnetic anisotropy (D = −14.5 cm−1) was established for iron compound 3. The cobalt coordination compounds 1 and 2 show SIM behavior under a 1500 Oe external magnetic field, with effective magnetization reversal barriers of 65(1) and 60(1) K for 1 and 2, respectively. The combination of Orbach and Raman relaxation mechanisms was shown to adequately describe the temperature dependence of relaxation times for 1 and 2. CASSCF/NEVPT2 calculations were performed to model the parameters of the effective spin Hamiltonian for the compounds under study.

The paper evaluates the behavior of a red-mud solid fraction in a thickener feeder cup, aiming to identify the main characteristics of particle distribution in the flocculation zone and to determine the dependencies affecting the further process taking place in the particle-free sedimentation zone in the thickener-thickening unit. This work used mathematical and numerical modeling to study the influence of such parameters as the flow rate of the feed pulp in the thickener, the flow rate of the flocculant, the density of pulp at the inlet to the unit, and the viscosity and temperature of the pulp on the particle-size distribution from under the feeder cup. The results and dependencies obtained are intended to be used as nominal values in the red-mud thickening process performed on a lab-scale unit.

Yarrowia lipolytica yeast is well known to be able to synthesize citric acid (CA) in large amounts. This study deals with CA biosynthesis, the production of biomass, as well as the accumulation and composition of proteins and lipids in Y. lipolytica VKM Y-2373 grown in media with glucose at different concentrations of ammonium sulfate (from 2 to 10 g/L). It was found that these concentrations of nitrogen source are limiting for the growth of Y. lipolytica and that nitrogen deficiency is the main cause of CA excretion. At the high concentration of (NH4)2SO4 (10 g/L), the accumulation of cell biomass, biomass yield (YX/S), and protein concentration was higher than in the medium with 2 g/L ammonium sulfate by 4.3 times, 143%, and 5.1 times, respectively. CA was accumulated in meaningful quantities only in media containing 3–10 g/L (NH4)2SO4 with the maximum concentration of CA (99.9 g/L) at 4 g/L ammonium sulfate. Also of interest is the technological mode with 6 g/L (NH4)2SO4, which is characterized by high productivity (1.11 g/L × h). It should be noted that biomass contains large amounts of essential amino acids and unsaturated fatty acids and can be used in food biotechnologies and agriculture.