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The Circular Electron Positron Collider (CEPC) is one of the future experiments which aims to precisely study the properties of the Higgs boson and to search for new physics beyond the Standard Model. To achieve that, it puts strict requirements on the particle reconstruction and identification ability of the CEPC detector. In order to obtain better particle identification performance, the drift chamber is chosen for the outer tracking detector which can provide information used by the cluster counting method for getting excellent particle identification performance. To evaluate the performance of the cluster counting method, it is necessary to develop a simulation tool that can be used to precisely simulate the response of the gaseous particle detector. This work proposes a new way to combine Geant4 and Garfield++ at the Geant4 step level for the drift chamber simulation. Due to the extreme time consumption of simulating the avalanche process in Garfield++, a fast signal response simulation method based on a neural network has been developed to replace detailed simulation. It shows the fast simulation can give consistent results with Garfield++ simulation and achieve the speedup by a factor of 200.

Rotator cuff (RC) tears are a common cause of upper extremity disability. Any tear size can result in subsequent muscle atrophy and fatty infiltration (FI). Preoperative muscle degeneration can predict repair and postoperative functional outcomes. Muscle residential fibro-adipogenic progenitors (FAPs) are found to be capable of differentiating into beige adipocytes that release factors to promote muscle growth. This study evaluated the regenerative potential of local cell transplantation of beige FAPs to mitigate muscle degeneration in a murine massive RC tear model. Beige FAPs were isolated from muscle in UCP-1 reporter mice by flow cytometry as UCP-1+ /Sca1+ /PDGFR+ /CD31- /CD45- /integrin α7- . C57/BL6J mice undergoing supraspinatus tendon tear with suprascapular nerve transection (TT + DN) received either no additional treatment, phosphate-buffered saline injection, or beige FAP injection 2 weeks after the initial injury. Forelimb gait analysis was used to assess shoulder function with DigiGait. Mice were sacrificed 6 weeks after cell transplantation. FI, fibrosis, fiber size, vascularity were analyzed and quantified via ImageJ. Our results showed that beige FAP transplantation significantly decreased fibrosis, FI, and atrophy, enhanced vascularization compared with saline injection and non-treatment groups. Beige FAP transplantation also significantly improved shoulder function as measured by gait analysis. This study suggests that beige-differentiated FAPs may serve as a treatment option for RC muscle atrophy and FI, thus improving shoulder function in patients with massive RC tendon tears. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:1159-1166, 2020.

The aim of the study was to investigate the clinicopathological characteristics and prognosis of proliferative glomerulonephritis with monoclonal immunoglobulin deposits (PGNMID) and determine the differences between PGNMID associated with extrarenal disease and without clear etiology as well as the differences between IgG1 and IgG3 subtypes.Data from 46 patients with PGNMID observed from January 2014 to September 2021 in Peking University First Hospital were retrospectively analyzed, including 36 patients without clear etiology (Group A) and 10 patients with extrarenal disease (Group B).At presentation patients showed proteinuria (95.7%), hematuria (89.1%), renal insufficiency (73.9%), and hypocomplementemia of C3 or C4 (35.6%). Monoclonal immunoglobulin or cell clones were detected in 22.2% of patients (10/45). The monoclonal immunoglobulins deposited in kidney were IgG3 in 40 patients, IgG1 in 5, and IgM in one. Monoclonal IgG1 deposits were more common in Group B than in Group A (4/10 vs. 1/36, p = 0.006). The intensity of glomerular C3 deposition and the frequency of subendothelial deposits in IgG3 subtype were significantly higher than those in IgG1 subtype. During a median follow-up time of 12.2 (range 1-61) months, a higher level of serum creatinine at biopsy and a higher percentage of global glomerulosclerosis were independent predictors of end-stage kidney disease.PGNMID associated with extrarenal disease was more likely to have monoclonal IgG1 deposits. PGNMID of IgG3 subtype differs from IgG1 subtype by higher intensity of glomerular C3 deposition and higher frequency of subendothelial deposits. Serum creatinine and global glomerulosclerosis were independent prognostic predictors of ESKD in PGNMID.

BackgroundEffective and accurate screening of oncological biomarkers in peripheral blood circulation plays an increasingly vital role in diagnosis and prognosis. High-sensitivity assays can effectively aid clinical decision-making and intervene in cancer in a localized status before they metastasize and become unmanageable. Meanwhile, it is equally pivotal to prevent overdiagnosis of non-life-threatening cancer by eliminating unnecessary treatment and repeated blood draws. Unfortunately, current clinical screening methodologies can hardly simultaneously attain sufficient sensitivity and specificity, especially under resource-restrained circumstances. To circumvent such limitations, particularly for cancer biomarkers from early-onset and recurrence, we aim to develop a universal plasmonic platform for clinical applications, which macroscopically amplifies multiplexed fluorescence signals in a broad spectral window readily adapts to current assay setups without sophisticated accessories or expertise at low cost. MethodsThe plasmonic substrate was chemically synthesized in situ at the solid-liquid interface by rationally screening a panel of reducing monosaccharides and tuning the redox reactions at various catalyst densities and precursor concentrations. The redox properties were studied by Benedict’s assay and electrochemistry. We systemically characterized the morphologies and optical properties of the engineered plasmonic Ag structures by scanning electron microscopy (SEM) and spectroscopy. The structure-fluorescence enhancement correlation was explicitly explained by the finite-difference time-domain (FDTD) simulation and a computational model for gap distribution. Next, we established an enhanced fluoroimmunoassay (eFIA) using a model biomarker for prostate cancer (PCa) and validated it in healthy and PCa cohorts. Prognosis was explored in patients subject to surgical and hormonal interventions following recommended PCa guidelines. ResultsThe monosaccharide-mediated redox reaction yielded a broad category of Ag structures, including sparsely dispersed nanoparticles of various sizes, semi-continuous nanoislands, and crackless continuous films. Optimal broad-spectral fluorescence enhancement from green to far-red was observed for the inhomogeneous, irregularly-shaped semi-continuous Ag nanoisland substrate (AgNIS), synthesized from a well-balanced redox reaction at a stable rate mediated by mannose. In addition, different local electric field intensity distributions in response to various incident excitations were observed at the nanoscale, elucidating the need for irregular and inhomogeneous structures. AgNIS enabled a maximized 54.7-fold macroscopically amplified fluorescence and long-lasting photostability. Point-of-care availability was fulfilled using a customized smartphone prototype with well-paired optics. The eFIA effectively detected the PCa marker in cell lines, xenograft tumors, and patient sera. The plasmonic platform rendered a diagnostic sensitivity of 86.0% and a specificity of 94.7% and capably staged high-grade PCa that the clinical gold standard test failed to stratify. Patient prognosis of surgical and hormonal interventions was non-invasively monitored following efficient medical interventions. The assay time was significantly curtailed on the plasmonic platform upon microwave irradiation. ConclusionsBy investigating the effects of monosaccharides on the seed-mediated chemical synthesis of plasmonic Ag structures, we deduced that potent multiplexed fluorescence enhancement originated from both an adequate reducing power and a steady reduction rate. Furthermore, the inhomogeneous structure with adequate medium gap distances afforded optimal multiwavelength fluorescence enhancement, thus empowering an effective eFIA for PCa. The clinically validated diagnostic and prognostic features, along with the low sample volume, point-of-care feasibility with a smartphone, and microwave-shortened assay time, warrant its potential clinical translation for widespread cancer biomarker analysis.

Energy and environmental pollution are major global challenges. This paper aims to examine the impact of China’s energy-consumption permit trading scheme (ECPT) on environmental pollution and the influence mechanisms. The study constructs provincial panel data for China from 2006 to 2020 and uses the difference-in-difference (DID) method to investigate the issue. Our results demonstrate that ECPT significantly curbs SO2 emissions, as confirmed by a series of statistical robustness tests. Specifically, the ECPT has significantly reduced SO2 by approximately 30.4%. Furthermore, the ECPT reduces SO2 emissions mainly by optimizing the structure of energy consumption and promoting technological innovation. In addition, the impact of the ECPT on SO2 emissions is more pronounced in the central and western provinces of China, and in provinces with lower levels of industrial structure and high environmental regulation intensity. This study provides a perspective from a developing country and makes an important contribution to the existing research by exploring the curbing effect of energy policy on SO2 emissions.

The fine structure of sweet tea polysaccharide (STP-60a) has been characterized. However, the biological activity of STP-60a has not been extensively explored. This study aims to evaluate the anti-aging activity of STP-60a using Caenorhabditis elegans (C. elegans) as a model and to investigate the underlying molecular mechanism. 400 μg/mL of STP-60a increased the mean lifespan of C. elegans by 22.88%, reduced the lipofuscin content by 33.01%, and improved the survival rate under heat stress and oxidative stress by 32.33% and 27.63%, respectively. Further research in lifespan-related mutants revealed that STP-60a exerted anti-aging effects mainly through insulin and mitochondrial signaling pathways. Through qRT-PCR and microscopic imaging of transgenic nematodes, we found that 400 μg/mL of STP-60a increased the expression of daf-16, skn-1, and hsf-1 downstream of the insulin pathway by 1.68-fold, 1.88-fold, and 1.03-fold, respectively, and promoted the accumulation of daf-16 and skn-1 in the nucleus. STP-60a also significantly regulated the function of the mitochondrial respiratory chain and unfolded protein recovery system. Furthermore, STP-60a activated the autophagy level in C. elegans, and the mutation of daf-2 or clk-1 inhibited the upregulation of autophagy genes by STP-60a, suggesting that autophagy acted as an effector of the insulin and mitochondrial pathways during STP-60a antiaging.

In this study, an inulin-type fructan (PGPI-1-a) was isolated from the roots of Platycodon grandiflorum. PGPI-1-a was composed of (2 → 1)-linked β-D-fructofuranose (Fruf) and a terminal α-d-glucopyranose (Glcp) with a molecular weight of 12.1 kDa. PM2.5 exposure has brought a great threat to human health in recent years. Therefore, this study explored the effect of PGPI-1-a on the intestinal microbial community structure of rats exposed to PM2.5 using the animal model of PM2.5 inhalation exposure. The results showed that PGPI-1-a could regulate the intestinal microbiota by partly restoring the perturbed levels of Peptoniphilaceae_[G-2] and Lachnospiraceae_[G-2] caused by PM2.5 exposure. In addition, the relative abundance of Butyrivibrio, a butyric acid-producing genera, significantly increased after PGPI-1-a intervention. These results indicated that PGPI-1-a could improve the imbalance of intestinal microbiota due to PM2.5 exposure to a certain extent.

Microenergy acoustic pulses (MAP) is a modified low-intensity extracorporeal shock wave therapy that currently used for treating musculoskeletal disorders. However, its function on muscle regeneration after ischemia-reperfusion injury (IRI) remains unknown. This study aimed to explore the effect of MAP on muscle injury after IRI and its underlying mechanisms. Ten-week-old C57BL/6J mice underwent unilateral hindlimb IRI followed with or without MAP treatment. Wet weight of tibialis anterior muscles at both injury and contralateral sides were measured followed with histology analysis at 3 weeks after IRI. In in vitro study, the myoblasts, endothelial cells and fibro-adipogenic progenitors (FAP) were treated with MAP. Cell proliferation and differentiation were assessed, and related gene expressions were measured by real-time PCR. Our results showed that MAP significantly increased the muscle weight and centrally nucleated regenerating muscle fiber size along with a trend in activating satellite cells. In vitro data indicated that MAP promoted myoblast proliferation and differentiation and endothelial cells migration. MAP also induced FAP brown/beige adipogenesis, a promyogenic phenotype of FAPs. Our findings demonstrate the beneficial function of MAP in promoting muscle regeneration after IR injury by inducing muscle stem cells proliferation and differentiation.

Warm- and cool-season turfgrasses were originated from different locations with contrasting heat tolerance. The molecular mechanisms of heat tolerance have not been extensively studied in turfgrass species. In this study, transcriptomic analysis showed that bermudagrass was more tolerant to heat stress as evidenced by lower contents of H

We present a new divergence method to estimated methane (CH4) emissions from satellite observed mean mixing ratio of methane (XCH4) by deriving the regional enhancement of XCH4 in the Planetary Boundary Layer (PBL). The applicability is proven by comparing the estimated emissions with its known emission inventory from a 3-month GEOS-Chem simulation. When applied to TROPOspheric Monitoring Instrument observations, sources from well-known oil/gas production areas, livestock farms and wetlands in Texas become clearly visible in the emission maps. The calculated yearly averaged total CH4 emission over the Permian Basin is 3.06 (2.82, 3.78) Tg a−1 for 2019, which is consistent with previous studies and double that of EDGAR v4.3.2 for 2012. Sensitivity tests on PBL heights, on the derived regional background and on wind speeds suggest our divergence method is quite robust. It is also a fast and simple method to estimate the CH4 emissions globally.

Background: Previous studies have found that the dissemination pattern and delivery mechanism of information can provide crucial resources and empowerment for individuals to the promotion of health literacy. The present study investigates how health information sources are associated with health literacy among older adults in west China, and tries to explain the mechanisms underlying the link between health information sources and health literacy in the Chinese context. Methods: The cross-sectional study employed a representative sample of 812 urban citizens aged 60 and older in 2017 in Western China. Results: We found that health information sources including healthcare practitioners (B = 4.577, p < 0.001), neighbors (B = 2.545, p < 0.05), newspapers (B = 4.280, p < 0.001), and television (B = 4.638, p < 0.001) were positively associated with health literacy. Additionally, age (B = −1.781, p < 0.001) was negatively associated with health literacy, and the socio-economic status factors including minority (B = −10.005, p < 0.001), financial strain status of perceived very difficult (B = −10.537, p < 0.001), primary school (B = 11.461, p < 0.001), junior high school (B = 18.016, p < 0.001), polytechnic school or senior high school (B = 21.905, p < 0.001), college and above (B = 23.433, p < 0.001) were significantly linked to health literacy, and suffering from chronic diseases (B = 3.430, p < 0.01) was also positively related to health literacy. Conclusions: Health information sources including healthcare practitioners, neighbors, newspapers, and television have a strong influence on health literacy, which implies that the four main types of sources are the important patterns of health information dissemination in the reinforcement of health literacy. In addition, the present findings also indicate age, minority and disease differences in health literacy and confirm the influence of enabling factors including educational attainment and financial strain on health literacy. Based on these findings and their implications, specific evidence is presented for the reinforcement of health literacy in interpersonal and mass communication, and in the educational and financial settings in the Chinese context. The present results also suggest that the age-specific, minority-specific and disease-specific measures should be taken to promote health literacy among older adults.

The Madin–Darby bovine kidney (MDBK) cell line is currently used for the production of bovine alphaherpesvirus-1 (BoHV-1) vaccine. For the purpose of vaccine manufacturing, suspension cells are preferred over adherent ones due to simplified sub-cultivation and an easier scale-up process, both of which could significantly reduce production cost. This study aimed to establish a procedure for the culture of BoHV-1 in the suspended MDBK cell line in serum-free medium. We screened several commercially available serum-free media and chose ST503 for subsequent experiments. We successfully adapted the adherent MDBK cells to suspended growth in ST503 in the absence of serum. The maximum density of suspension-adapted MDBK cells could reach 2.5 × 107 cells/mL in ST503 medium with optimal conditions. The average size of suspension-adapted cells increased to 18 ± 1 µm from 16 ± 1 µm. Moreover, we examined tumorigenicity of the suspended cells and found no sign of tumorigenicity post adaptation. Next, we developed a protocol for the culture of BoHV-1 in the cell line described above and found that ultrasonic treatment could facilitate virus release and enhance virus yield by 11-fold, with the virus titer reaching 8.0 ± 0.2 log10TCID50/mL. Most importantly, the prototype inactivated BoHV-1 vaccine we generated using the suspension cultures of MDBK cells induced neutralizing antibodies to a titer comparable to that of the commercial inactivated BoHV-1 vaccine. Overall, we established and optimized a protocol for the production of inactivated BoHV-1 vaccine in MDBK cells adapted for suspension culture, which provides insights for future large-scale manufacturing of BoHV-1 vaccine.

Ultrasound is a widely used technology for diagnosing developmental dysplasia of the hip (DDH) because it does not use radiation. Due to its low cost and convenience, 2-D ultrasound is still the most common examination in DDH diagnosis. In clinical usage, the complexity of both ultrasound image standardization and measurement leads to a high error rate for sonographers. The automatic segmentation results of key structures in the hip joint can be used to develop a standard plane detection method that helps sonographers decrease the error rate. However, current automatic segmentation methods still face challenges in robustness and accuracy. Thus, we propose a neonatal hip bone segmentation network (NHBS-Net) for the first time for the segmentation of seven key structures. We design three improvements, an enhanced dual attention module, a two-class feature fusion module, and a coordinate convolution output head, to help segment different structures. Compared with current state-of-the-art networks, NHBS-Net gains outstanding performance accuracy and generalizability, as shown in the experiments. Additionally, image standardization is a common need in ultrasonography. The ability of segmentation-based standard plane detection is tested on a 50-image standard dataset. The experiments show that our method can help healthcare workers decrease their error rate from 6%-10% to 2%. In addition, the segmentation performance in another ultrasound dataset (fetal heart) demonstrates the ability of our network.

Background Pneumonia is a frequent infectious disease that mainly affects the children and the global death rate is nearly 19% among children at the below 5 age. β-caryophyllene is an active compound, mainly occurs in the spices and it possesses immense biological activities. Objective This investigation deliberated to scrutinize the beneficial actions of β-caryophyllene against the M. pneumoniae induced pneumonia. Methods The pneumonia was stimulated to the BALB/c mice by infecting them with 100 µl of M. pneumonia for 2 days via nasal drops with the concomitant treatment with 20 mg/kg of β-caryophyllene. The total cells in the BALF of test mice were counted by using the Neuber chamber. The total protein and the pro-inflammatory cytokines status were examined by using the commercial ELISA kits. The PCR technique was used to measure the M. pneumoniae bacterial load. The NF-ƙB expression was investigated using western blotting. The lung tissues were analyzed microscopically. Results The β-caryophyllene notably diminished the total protein status, total cell count, and bacterial load in the pneumonia provoked mice. The marked reduction in the status of pro-inflammatory regulators was seen in the β-caryophyllene supplemented pneumonia mice. β-caryophyllene also down-regulated the expression of NF-ƙB thereby reduced the lung inflammation and tissue damages as seen in the result of histological analysis. Conclusion These findings were confirmed the therapeutic potential of β-caryophyllene against the M. pneumoniae-activated pneumonia in animals.

Background Context The association between low back pain and lumbar disc degeneration is not fully characterized. One potentially overlooked factor of this process is the lumbar multifidus, which plays a role in segmental stabilization and locomotion. Previous reports have shown multifidus degeneration to be associated with disc degeneration. The goal of this study was to develop a mouse model of advanced lumbar disc degeneration to recapitulate the pathology of the human multifidus in patients with lumbar disc degeneration and low back pain. Methods C57BL/6 mice underwent a left anterolateral approach to the lumbar spine and disc puncture with a micro scalpel at L5/6 and L6/S1. Mice underwent behavioral analysis and functional gait testing. A subset of mice underwent 14T T2-weighted MRI to assess disc degeneration and paraspinal muscle quality. At 6 and 15 weeks, mice were sacrificed, and the multifidus muscles were harvested and divided into proximal and distal segments relative to disc injury. Histological analysis was performed to assess muscle degeneration, fiber type, and macrophage density. Fibroadipogenic progenitors (FAPs) were isolated for gene expression analysis with qPCR. Results MRI demonstrated decreased intervertebral disc signal and paraspinal muscle atrophy at 6 weeks, with progressive degeneration and atrophy at 15 weeks. Disc injury resulted in delayed functional recovery and impaired gait. Histology demonstrated progressive multifidus fibrofatty degeneration between 6 and 15 weeks. CD68+ macrophage density was increased at 6 weeks but not 15 weeks. FAPs exhibited increased fibrotic and adipogenic gene expression at 6 weeks compared to sham with less of a difference in gene expression by 15 weeks. Conclusions We have developed a mouse model of disc injury-mediated paraspinal muscle degeneration that recapitulates features of degenerative muscle pathology observed in patients with lumbar disc degeneration, and highlights the role of FAPs in mediating fibrofatty muscle degeneration after disc injury.

In order to combat environmental pollution, China enacted the Environmental Protection Tax Law in early 2018. Yet the impacts of the environmental tax on individual regions with different socioeconomic statuses, which are crucial for social justice and public acceptance, remain unclear. Based on a Multi-Regional Input-Output (MRIO) Table and a nationally regulated tax payment calculation method, this study analyzes the distributional impacts of the environmental tax due to each province’s consumption from both inter-provincial and rural-urban aspects. The national tax revenue based on the current levy mechanism is estimated to be only one seventh of the economic loss from premature mortality caused by ambient particulate matter (PM2.5). The taxation may slightly alleviate urban-rural inequality but may not be helpful with reducing inter-provincial inequality. We further analyze two alternative levy mechanisms. If each province imposes taxes to products it consumes (rather than produces, as in the current mechanism), with the tax rate linearly dependent on its per capita consumption expenditure, this would moderately increase the national tax revenue and effectively reduce inter-provincial inequality. To better compensate for the economic cost of air pollution and reduce regional inequality, it would be beneficial to increase the tax rate nationwide and implement a levy mechanism based on provincially differentiated levels of consumption and economic status.

Emission datasets of nitrogen oxides ( NOx ) at high horizontal resolutions (e.g., 0.05 ∘ × 0.05 ∘ ) are crucial for understanding human influences at fine scales, air quality studies, and pollution control. Yet high-resolution emission data are often missing or contain large uncertainties especially for the developing regions. Taking advantage of long-term satellite measurements of nitrogen dioxide ( NO2 ), here we develop a computationally efficient method of estimating NOx emissions in major urban areas at the 0.05 ∘ × 0.05 ∘ resolution. The top-down inversion method accounts for the nonlinear effects of horizontal transport, chemical loss, and deposition. We construct a two-dimensional Peking University High-resolution Lifetime-Emission-Transport model (PHLET), its adjoint model (PHLET-A), and a satellite conversion matrix approach to relate emissions, lifetimes, simulated NO2 , and satellite NO2 data. The inversion method is applied to the summer months of 2012–2015 in the Yangtze River Delta (YRD; 29–34 ∘ N, 118–123 ∘ E) area, a major polluted region of China, using the NO2 vertical column density data from the Peking University Ozone Monitoring Instrument NO2 product (POMINO). A systematic analysis of inversion errors is performed, including using an independent test based on GEOS-Chem simulations. Across the YRD area, the summer average emissions obtained in this work range from 0 to 15.3 kg km −2 h −1 , and the lifetimes (due to chemical loss and deposition) range from 0.6 to 3.3 h. Our emission dataset reveals fine-scale spatial information related to nighttime light, population density, road network, maritime shipping, and land use (from a Google Earth photo). We further compare our emissions with multiple inventories. Many of the fine-scale emission structures are not well represented or not included in the widely used Multi-scale Emissions Inventory of China (MEIC).

Fetus death risk reduction is included in the United Nations Sustainable Development Goals. However, little is known about how missed abortion in the first trimester (MAFT) is related to maternal air pollution exposure. We quantify the link between air pollution exposure and MAFT in Beijing, China, a region with severe MAFT and air quality problems. We analyse the records of 255,668 pregnant women from 2009 to 2017 and contrast them with maternal exposure to air pollutants (particulate matter PM2.5, SO2, O3 and CO). We adjust for confounding factors such as sociodemographic characteristics, spatial autocorrelation and ambient temperature. We find that, for all four pollutants, an increased risk of MAFT is associated with rises in pollutant concentrations and the adjusted odds ratios (ORs) of these associations increase with higher concentrations. For example, the adjusted OR of MAFT risk for a 10.0 μg m−3 increase in SO2 exposure is between 1.29 and 1.41 at concentrations of 7.1–19.5 μg m−3; it drops to 1.17 below this range and rises to 1.52 above it at higher SO2 concentrations. This means that the risk increase is not linear but becomes more severe the higher the pollutant concentration. The findings provide evidence linking fetus disease burden and maternal air pollution exposure. A study of over 250,000 pregnant women in Beijing, China, finds that missed abortion is associated with the concentration of four major air pollutants. The association is not linear but becomes stronger with higher concentrations.

The physiochemical properties of nanoparticles (NPs), including surface charge, will affect their uptake, transformation, translocation, and final fate in the environment. In this study, we compared the phytoxoxicity and transport behaviors of nano CeO2 (nCeO2) functionalized with positively charged (Cs-nCeO2) and negatively charged (PAA-nCeO2) coatings. Cucumber seedlings were hydroponically exposed to 0-1000 mg/L of Cs-nCeO2 and PAA-nCeO2 for 14 days and the contents, distribution, translocation, and transformation of Ce in plants were analyzed using inductively coupled plasma mass spectrometry, micro X-ray fluorescence (μ-XRF), and X-ray absorption near-edge spectroscopy (XANES), respectively. Results showed that the seedling growth and Ce contents in plant tissues were functions of exposure concentrations and surface charge. Cs-nCeO2 was adsorbed strongly on a negatively charged root surface, which led to significantly higher Ce contents in the roots and lower translocation factors of Ce from the roots to shoots in Cs-nCeO2 group than in PAA-nCeO2 group. The results of μ-XRF showed that Ce elements were mainly accumulated at the root tips and lateral roots, as well as in the veins and at the edge of leaves. XANES results revealed that the proportion of Ce(III) was comparable in the plant tissues of the two groups. We speculated that Cs-nCeO2 and PAA-nCeO2 were partially dissolved under the effect of root exudates, releasing Ce3+ ions as a result. Then, the Ce3+ ions were transported upward in the form of Ce(III) complexes along the vascular bundles and eventually accumulated in the veins. The other portion of Cs-nCeO2 and PAA-nCeO2 entered the roots through the gap of a Casparian strip at root tips/lateral roots and was transported upward as intact NPs and finally accumulated at the edge of the blade. This study will greatly advance our information on how the properties of NPs influence their phytotoxicity, uptake, and subsequent trophic transfer in terrestrial food webs.

There is insufficient evidence on the relationship between air pollution and mortality from cardiovascular disease (CVD) in northeast China. Here, we explored the short-term effects of air pollution on CVD mortality and preliminarily investigated differences in population susceptibility to air pollution in Shenyang, China. CVD mortality, air pollution, and meteorological data during 2013–2016 were obtained. Time-series analysis was applied to evaluate the association between air pollution and daily CVD mortality with different lag structures. In the single-pollutant model, each 10 μg/m3 increase in PM2.5, PM10, SO2, NO2, and O3 concentrations and 1 mg/m3 increase in CO concentrations at lag0 (same day) was significantly associated with an increase of 0.40% (95% confidence interval, 0.22–0.59%), 0.26% (0.12–0.40%), 0.43% (0.16–0.70%), 0.90% (0.14–1.67%), 0.76% (0.21–1.32%), and 3.33% (0.97–5.75%), respectively, in overall CVD mortality. Susceptibility to air pollutants was higher among females, elderly people, and ischemic heart disease patients. Furthermore, air pollution effects on CVD mortality were 2–8 times greater during the non-heating period. In conclusion, the air pollutants PM2.5, PM10, SO2, NO2, O3, and CO showed significant positive effects on CVD mortality in Shenyang, China. These findings highlight the adverse effects of air pollution and suggest the need for personal protective equipment and reduction of air pollution sources.

There exist substantial differences in top-of-atmosphere direct radiative forcing of aerosols due to a region's economic production (RFp) and consumption (RFc), in the context of economic globalization, trade and globalizing air pollution. Yet an explicit systematic analysis of all socioeconomic and atmospheric factors determining the RF difference is lacking. Here, we evaluate five socioeconomic (population, per capita output, emission intensity) and atmospheric (chemical efficiency and radiative efficiency) factors that determine a region's RFp, RFc and their difference. We consider the RF of secondary inorganic aerosols, primary organic aerosols and black carbon by 10 regions worldwide in 2007. The population size varies by a factor of nine across the regions, and per capita output by 40 times from both production- and consumption-based perspectives. The cross-regional spread reaches a factor of 181 (species dependent) for production-based emission intensity and a factor of 96 for consumption-based intensity. From one region to another, production-based chemical efficiency changes within a factor of 5 and consumption-based efficiency within a factor of 3.5. Radiative efficiency varies slightly across the regions (within 2) from both production- and consumption-based perspectives. Although socioeconomic factors are often a greater driver for the difference between a source region's RFp and RFc, the atmospheric factors are also important for many source regions and species. Our results contribute to regional attribution of climate change and establishment of effective international collaborative mitigation strategies.

Satellite retrieval of vertical column densities (VCDs) of tropospheric nitrogen dioxide (NO2) is critical for NOx pollution and impact evaluation. For regions with high aerosol loadings, the retrieval accuracy is greatly affected by whether aerosol optical effects are treated implicitly (as additional “effective” clouds) or explicitly, among other factors. Our previous POMINO algorithm explicitly accounts for aerosol effects to improve the retrieval, especially in polluted situations over China, by using aerosol information from GEOS-Chem simulations with further monthly constraints by MODIS/Aqua aerosol optical depth (AOD) data. Here we present a major algorithm update, POMINO v1.1, by constructing a monthly climatological dataset of aerosol extinction profiles, based on level 2 CALIOP/CALIPSO data over 2007–2015, to better constrain the modeled aerosol vertical profiles. We find that GEOS-Chem captures the month-to-month variation in CALIOP aerosol layer height (ALH) but with a systematic underestimate by about 300–600 m (season and location dependent), due to a too strong negative vertical gradient of extinction above 1 km. Correcting the model aerosol extinction profiles results in small changes in retrieved cloud fraction, increases in cloud-top pressure (within 2 %–6 % in most cases), and increases in tropospheric NO2 VCD by 4 %–16 % over China on a monthly basis in 2012. The improved NO2 VCDs (in POMINO v1.1) are more consistent with independent ground-based MAX-DOAS observations (R2=0.80, NMB = −3.4 %, for 162 pixels in 49 days) than POMINO (R2=0.80, NMB = −9.6 %), DOMINO v2 (R2=0.68, NMB = −2.1 %), and QA4ECV (R2=0.75, NMB = −22.0 %) are. Especially on haze days, R2 reaches 0.76 for POMINO v1.1, much higher than that for POMINO (0.68), DOMINO v2 (0.38), and QA4ECV (0.34). Furthermore, the increase in cloud pressure likely reveals a more realistic vertical relationship between cloud and aerosol layers, with aerosols situated above the clouds in certain months instead of always below the clouds. The POMINO v1.1 algorithm is a core step towards our next public release of the data product (POMINO v2), and it will also be applied to the recently launched S5P-TROPOMI sensor.

e23522 Background: High-dose methotrexate (HDMTX) is a renally eliminated and nephrotoxic therapy for osteosarcoma (OS). Serum cystatin C (Cys C) has emerged as an alternative to serum creatinine (Scr) for estimation of glomerular filtration rate (GFR). We aim to analyze the value of the baseline Cys C in predicting MTX elimination in patients with OS. Methods: All consecutive patients who were treated with HDMTX in Shanghai Jiao Tong University Affiliated Sixth People¡¯s Hospital, between November 2011 and April 2018, were retrospectively reviewed. The baseline GFR was calculated by three different equations based on the Scr, Cys C and their combination. MTX concentrations at 24, 48 and 72 hours after the termination of infusion were routinely monitored using high performance liquid chromatography. Results: A total of 158 courses of MTX infusion were administered. The incidences of delayed excretion of MTX at 24, 48, and 72 hours (serum concentrations at 24 h > 10 μmol/L, 48h > 1 μmol/L, 72h >0.1 μmol/L) were 62.3%, 44.9% and 29.7%, respectively. The baseline Scr in the delayed excretion (DE) group were significantly lower than that in the normal excretion (NE) group at all three time points (24h: 58.8 µmol/L vs 47.86 µmol/L, P£¼0.05; 48h: 59.02 µmol/L vs 47.48 µmol/L, P£¼0.05; 72h: 62.29 µmol/L vs 51.51 µmol/L, P£¼0.05). However, the baseline Cys C in the DE group were similar to that in the NE group at all three time points (24h: 0.96 mg/L vs 0.90 mg/L, P = 0.11; 48h: 0.96 mg/L vs 0.92 mg/L, P = 0.17; 72h: 0.96 mg/L vs 0.93 mg/L, P£¾0.05). The Scr-based estimated GFR in the DE group were significantly lower than that in the NE group at all three time points (24h: 148.42 mL/min vs 186.79 mL/min, P£¼0.05; 48h: 147.24 mL/min vs 188.82 mL/min, P£¼0.05; 72h: 142.95 mL/min vs 171.04 mL/min, P£¼0.05). The Scr- and Cys C-based estimated GFR in the DE group were also significantly lower than that in the NE group at all three time points (24h: 140.33 mL/min vs 161.39 mL/min, P£¼0.05; 48h: 141.69 mL/min vs 159.04 mL/min, P£¼0.05; 72h: 138.33 mL/min vs 152.31 mL/min, P£¼0.05). However, the Cys C based estimated GFR in the DE group were similar to that in the NE group at all three time points (24h: 95.95 mL/min vs 101.31 mL/min, P = 0.183; 48h: 97.49 mL/min vs 98.64 mL/min, P = 0.763; 72h: 95.30 mL/min vs 99.06 mL/min, P = 0.375). Conclusions: Baseline Cys C alone is unable to predict MTX elimination in patients with OS.[Table: see text]

2019 the outbreak of New Coronavirus pneumonia COVID-19 in Wuhan is characterized by high infectivity, long incubation period and high mortality rate. It seriously interferes with the normal production activities during the outbreak. Therefore, it is of great significance to study its transmission mechanism and the future development trend. In this paper, the SEIR model of new coronavirus was established, and the value assignment method and least square method were used to solve the model. The fitting results and the real value had a good match, indicating that the model was more reasonable. The epidemic data of Wuhan, Guangdong and Brazil were analyzed and predicted respectively. The analysis found that the epidemic situation in Wuhan was basically stable; the epidemic situation in Guangdong was relatively late, and the control was relatively timely. The epidemic situation in Brazil started in the middle of March, so the mortality rate continued to rise from March to April, but the cure rate remained low. The epidemic prevention and control suggestions were put forward to Wuhan, Guangdong and Brazil respectively. The theoretical analysis of the model shows that prevention and control isolation and medical tracking isolation play an important role in inhibiting the large-scale spread of the epidemic. In addition, individuals improve their awareness of prevention and take strict self-protection measures to curb the increase in the number of infected people. The SEIR epidemic model of the new coronavirus established in this paper can be used for covid-19 The purpose of this study is to provide theoretical support for making future intervention decisions

Bladder cancer represents a highly heterogeneous disease characterized by distinct histological, molecular and clinical phenotypes, and a detailed analysis of tumor cell invasion and crosstalks within bladder tumor cells has not been determined. Here, we applied droplet-based single-cell RNA sequencing (scRNA-seq) to acquire transcriptional profiles of 36 619 single cells isolated from seven patients. Single cell transcriptional profiles matched well with the pathological basal/luminal subtypes. Notably, in T1 tumors diagnosed as luminal subtype, basal cells displayed characteristics of epithelial-mesenchymal transition (EMT) and mainly located at the tumor-stromal interface as well as micrometastases in the lamina propria. In one T3 tumor, muscle-invasive tumor showed significantly higher expression of cancer stem cell markers SOX9 and SOX2 than the primary tumor. We additionally analyzed communications between tumor cells and demonstrated its relevance to basal/luminal phenotypes. Overall, our single-cell study provides a deeper insight into the tumor cell heterogeneity associated with bladder cancer progression.

Mycoplasma bovis is a significant bacterial pathogen which is able to persist in cattle and cause chronic diseases. This phenomenon may relate to M. bovis evading the immune system of the host. Immunoglobulin-binding proteins are widely distributed in a variety of pathogenic bacteria, including some Mycoplasma species. These proteins are considered to help the bacteria evade the immune response of the host. Here we found M. bovis strain PG45 can bind to IgG from several animals. MBOVPG45_0375 encodes a putative membrane protein, has strong amino acid sequence similarity with Immunoglobulin G-binding protein in Mycoplasma mycoides subsp. capri. Hence, we constructed recombinant MBOVPG45_0375 (r0375) in the Escherichia coli expression system and demonstrated that r0375 can bind to IgG non-immunologically rather than specific binding similar to interaction of antigen and antibody. Moreover, r0375 can bind to the Fab fragment of IgG. Also, the binding of r0375 and IgG inhibits the formation of antigen-antibody union. Furthermore, MBOVPG45_0376 encodes an IgG-cleaving protein of M. bovis strain PG45. Nevertheless, r0375 binding to IgG is required for the cleavage activity of recombinant 0376 (r0376). The activity of r0376 is also affected by incubation time and temperature. In addition, we found both MBOVPG45_0375 and MBOVPG45_0376 are membrane proteins of M. bovis strain PG45. These results about MBOVPG45_0375 as an IgG-binding protein and MBOVPG45_0376 as an IgG-cleaving protein offer a new insight into the interaction between M. bovis and its host.

Global tropospheric nitrogen dioxide (NO

The high-resolution Tropospheric Monitoring Instrument (TROPOMI) satellite observations of atmospheric methane offer a powerful tool to identify emission hot spots and quantify regional emissions. The divergence of horizontal fluxes of NO2 has already been proven to be an efficient way to resolve and quantify high sources on a global scale. Since the lifetime of CH4 is in the order of 10 years, the sinks can be ignored at the synoptic time scale which makes the divergence method even more applicable to CH4 than to short-lived NO2. Because plumes of newly emitted CH4 disperse within the Planetary Boundary Layer (PBL), we first convert the satellite observed total column average (XCH4) to a regional enhancement of methane in the PBL (∆XCH4_PBL) by using the CAMS global methane background reanalysis fields above the PBL. These model fields represent the transport- and chemically-modulated large-scale distribution of methane. Secondly, the divergence of ∆XCH4_PBL is derived by the use of the wind speeds halfway within the PBL. Based on the divergence, methane emissions are estimated on a 0.25°× 0.25° grid. We tested our new method for Texas in the United States and quantified methane emissions from the well-known oil-gas fields in the Permian Basin, as well as from – less well quantitatively established – oil-gas fields located in southern coastal areas. Compared to traditional inverse methods, our method is not restricted by an a priori emission inventory and so far unidentified local sources (i.e. emissions from livestock in feed yards) may be found. Due to its computational efficiency, the method might be applied in the near future globally on the current spatial resolution.

The influence of morphology on the biological effects of nanomaterials (NMs) has not been well understood. In the present study, we compared the phytotoxicity of rod-shaped nano-cerium dioxide (R-CeO2) and nano-cerium phosphate (R-CePO4) to lettuce plants. The results showed that R-CeO2 significantly inhibited the root elongation of lettuce, induced oxidative damages, and caused cell death, while R-CePO4 was nontoxic to lettuce. The different distribution and speciation of Ce in plant tissues were determined by transmission electron microscopy (TEM) and X-ray absorption near edge spectroscopy (XANES) combined with linear combination fitting (LCF). The results showed that in the R-CeO2 group, part of Ce was transformed from Ce(IV) to Ce(III), while only Ce(III) was present in the R-CePO4 group. When interacting with plants, R-CeO2 is easier to be dissolved and transformed than R-CePO4, which might be the reason for their different phytotoxicity. Although both are Ce-based NMs and have the same morphology, the toxicity of R-CeO2 seems to come from the released Ce3+ ions rather than its shape. This research emphasizes the importance of chemical composition and reactivity of NMs to their toxicological effects.

BackgroundSpace flight is a special abiotic stress condition. Due to the development of space technology, its potential value has received widespread attention. Space mutation breeding is one of the important methods for human beings to solve food security. However, the molecular mechanism of space mutagenesis is still not very clear.ResultIn this study, two kinds of rice, Dongnong423 (DN3) and Dongnong416 (DN6), were carried on the SJ-10 retractable satellite for 12.5 days in orbit, returned to the ground and planted in the field until the three-leaf (TLP) and tillering stage (TS). The results of antioxidant enzyme activity, soluble sugar, and electron leakage rate revealed that the space environment caused the stress response of rice. The TLP and TS of DN3 identified 113 and 58 different metabolites, respectively. The TLP and TS of DN6 identified 107 and 77 different metabolites, respectively. These metabolites include amino acids, sugars, fatty acids, organic acids and secondary metabolites. We used qRT-PCR technology to explore the changes of enzyme genes in the TCA cycle and amino acid metabolism pathway. Combined with the results of metabolomics, we determined that during the TLP, the TCA cycle rate of DN3 was inhibited and amino acid metabolism was activated, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. In TS, the TCA cycle rate of DN3 was inhibited, and amino acid metabolism was not significantly changed, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. These results suggest that the space environment has different stress response mechanisms to the two rice varieties, and these differences may be reflected in energy consumption and compound biosynthesis.ConclusionsThis research uses metabolomics for the first time to explore the effects of space flight team rice. This research provides new insights for further exploring the effects of space biology and space mutation breeding.

The stellar initial mass function (IMF) is fundamental for many areas of astrophysics, but its origin remains poorly understood. It may be inherited from the core mass function (CMF) or arise as a result of more chaotic, competitive accretion. Dense, gravitationally bound cores are seen in molecular clouds and some observations have suggested that the CMF is similar in shape to the IMF, though translated to higher masses by a factor of $\sim3$. Here we measure the CMF in 28 dense clumps within 3.5 kpc that are likely to be central regions of massive protoclusters, observed via $1.3\:{\rm{mm}}$ dust continuum emission by the ALMAGAL project. We identify 222 cores using the dendrogram algorithm with masses ranging from 0.04 to $252\:M_{\odot}$. We apply completeness corrections for flux and number recovery, estimated from core insertion and recovery experiments. At higher masses, the final derived CMF is well described by a single power law of the form $dN/d\:{\textrm{log}}\:M\propto\:M^{-\alpha}$ with $\alpha\simeq0.94\pm0.08$. However, we find evidence of a break in this power-law behavior between $\sim5$ and $15\:M_{\odot}$, which is, to our knowledge, the first time such a break has been found in distant ($\gtrsim 1$ kpc) regions by ALMA. We compare this massive protocluster CMF with those derived using the same methods in the G286 protocluster and a sample of Infrared Dark Clouds. The massive protocluster CMF is significantly different, i.e., containing more massive cores, which is a potential indication of the role of environment on the CMF and IMF., Comment: Accepted for publication in ApJ, 25 pages, 10 figures, 4 tables

Following the COVID-19 outbreak, unlike the plunge in traditional mainstream assets, crypto assets have performed very well. Indices that reflect changes in the crypto assets market have also grown in recent years. However, in terms of cryptocurrency index-related studies, there are not many, and the period analyzed is mostly three years. This study analyzes historical data from July 2014 to April 2020 based on the cryptocurrency index CRIX and six other traditional mainstream assets to verify the impact of crypto assets on traditional portfolios. By using the DCC-GARCH model, this study finds out the low dynamic correlation between the crypto assets and traditional ones. Furtherly, by using the mean–variance model, Cornish-Fisher expansion and T-copula CVaR approach to check the frontier line and portfolio performance, this study finds out that crypto assets have the potential to improve the risk-return characteristics of traditional portfolios.

To protect walnut peptides from harsh external environments during their storage and digestion, proliposomes loaded with walnut peptides were fabricated using sucrose, trehalose, and mannitol as carriers and lyoprotectants. The physicochemical properties, environmental stability, antioxidant/antibacterial activities, and digestion in vitro of the proliposomes were evaluated. The freshly prepared liposomes were uniform in size, but the hydrated proliposomes showed a more uneven size distribution. The lyoprotectants helped maintain favorable liposome shape during lyophilization. Alongside the lyoprotectants, the walnut peptides further stabilized the lipid bilayer. Proliposomes encapsulation didn't impact the peptides' antioxidant activity. Furthermore, walnut peptides-loaded proliposomes exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The proliposomes were stable during gastric-phase digestion. The lyoprotectants changed the free fatty acid release behaviors of the proliposomes. These characteristics suggest potential applications for proliposomes as effective delivery systems for biopeptides in food stuffs, thereby protecting bioactivities during storage and passage through the gastrointestinal tract.

With the advancement of science and technology, humans are chronically exposed to ionizing radiation. It is crucial to look for efficient and low-toxic anti-radiation agents. Through preliminary screening, we found that Acanthopanax senticosus polysaccharide (ASPS) played a major role in regulating immune damage caused by radiation. The objective of this study was to apply the Caenorhabditis elegans—P. aeruginosa (PA14) infection model to illuminate the mechanism of ASPS increasing the pathogen resistance of radiation-damaged nematodes. Results indicated that ASPS (1 mg/mL) significantly enhanced the pathogen resistance of radiation-damaged nematodes by directly elevating the immune response of nematodes rather than by affecting the bacterial activity. Through further research on the p38 MAPK signaling pathway and related mutants, we found that ASPS functioned by the p38 MAPK pathway in the intestine, and SKN-1, ATF-7 as the downstream targets of PMK-1 participated the regulation of ASPS. In addition, ASPS markedly alleviated the stress status of damaged nematodes by regulating oxidative stress. Collectively, our findings suggest that ASPS enhances the pathogen resistance of radiation-damaged nematodes through the intestinal p38MAPK-SKN-1/ATF-7 pathway and stress response.

Identifying the financial fraud behavior of listed companies in a timely manner and helping investors avoid risks is a key measure to promote the healthy development of the capital market. Aiming at the lag of traditional fraud identification method, a text classification model suitable for Chinese capital market is constructed based on user generated content (UGC) on social media platform. To detect fraudulent companies,TF-IDF features, topic features and explosive news quantity features are extracted guided by systemic functional linguistics (SFL) theory. Empirical analysis is conducted by crawling the news and comments of 124 companies from Eastmoney and JRJ.com.The empirical analysis results show that the model constructed by the article can effectively extract the implicit information in unstructured data and improve the timeliness of screening fraudulent behaviors of listed companies. The topic and keyword features in text comments play an important role in distinguishing the fraud of listed companies. On the one hand, it helps individual and institutional investors avoid investment traps, on the other hand, it helps regulators detect companies with fraudulent potential in time to prevent market risks.

To study the early phases of massive star formation, we present ALMA observations of SiO(5-4) emission and VLA observations of 6 cm continuum emission towards 32 Infrared Dark Cloud (IRDC) clumps, spatially resolved down to $\lesssim 0.05$ pc. Out of the 32 clumps, we detect SiO emission in 20 clumps, and in 11 of them the SiO emission is relatively strong and likely tracing protostellar outflows. Some SiO outflows are collimated, while others are less ordered. For the six strongest SiO outflows, we estimate basic outflow properties. In our entire sample, where there is SiO emission, we find 1.3 mm continuum and infrared emission nearby, but not vice versa. We build the spectral energy distributions (SEDs) of cores with 1.3 mm continuum emission and fit them with radiative transfer (RT) models. The low luminosities and stellar masses returned by SED fitting suggest these are early stage protostars. We see a slight trend of increasing SiO line luminosity with bolometric luminosity, which suggests more powerful shocks in the vicinity of more massive YSOs. We do not see a clear relation between the SiO luminosity and the evolutionary stage indicated by $L/M$. We conclude that as a protostar approaches a bolometric luminosity of $\sim 10^2 \: L_{\odot}$, the shocks in the outflow are generally strong enough to form SiO emission. The VLA 6 cm observations toward the 15 clumps with the strongest SiO emission detect emission in four clumps, which is likely shock ionized jets associated with the more massive ones of these protostellar cores., 42 pages, 18 figures, accepted to ApJ

Background Volumetric muscle loss (VML) often leads to chronic muscle weakness, impaired limb function, and even permanent disability. Recent studies suggest muscle residential fibro/adipogenic progenitors (FAPs) can adopt novel beige fat differentiation promoting muscle regeneration. The goal of this study is to define the role of FAP beige adipogenesis in muscle regeneration after VML in a mouse model.Methods Three months old male C57BL/6J mice, PDGFRα-GFP reporter mice, UCP-1 reporter mice, PDGFRα-CREERT/DTA inducible FAP depletion mice and NSG immune-deficient mice were used in this study. Volumetric muscle loss (VML) was created on tibialis anterior (TA) muscle with a punch. To induce FAP beige adipogenesis, Amibegron, a beta 3 adrenergic receptor (B3AR) agonist was administered to mice with I.P. injection. In a separate group, murine and human beige adipogenic FAPs was transplanted to mouse muscle after VML. Limb function was measured with gait analysis at 2 and 6 weeks after VML. Muscle histology and FAP gene expression analysis was also conducted at 2 and 6 weeks after VML.Results After VML, we observed robust proliferation of FAPs in PDGFRα-GFP reporter mice. PDGFRα-CREERT/DTA mice inducible FAP depletion mice showed reduced muscle regeneration after FAPs are depleted, suggesting a positive role of FAP in muscle regeneration after VML. Both Amibegron treatment and beige FAP transplantation significantly improved muscle regeneration and limb function after VML.Conclusion Stimulating FAP beige adipogenesis with B3AR agonists or transplantation of beige adipogenic FAPs could serve as new strategies in treating VML.

We report results of 0.05"-resolution observations toward the O-type proto-binary system IRAS 16547-4247 with the Atacama Large Millimeter/submillimeter Array (ALMA). We present dynamical and chemical structures of the circumbinary disk, circumstellar disks, outflows and jets, illustrated by multi-wavelength continuum and various molecular lines. In particular, we detect sodium chloride, silicon compounds, and vibrationally-excited water lines as probes of the individual protostellar disks at a scale of 100 au. These are complementary to typical hot-core molecules tracing the circumbinary structures on a 1000-au scale. The H2O line tracing inner-disks has an upper-state energy of Eu/k>3000K, indicating a high temperature of the disks. On the other hand, despite the detected transitions of NaCl, SiO, and SiS not necessarily having high upper-state energies, they are enhanced only in the vicinity of the protostars. We interpret that these molecules are the products of dust destruction, which only happens in the inner disks. This is the second detection of alkali metal halide in protostellar systems after the case of the disk of Orion Source I, and also one of few massive protostellar disks associated with high-energy transition water and silicon compounds. These new results suggest these "hot-disk" lines may be common in innermost disks around massive protostars, and have great potential for future research of massive star formation. We also tentatively find that the twin disks are counter-rotating, which might give a hint of the origin of the massive proto-binary system IRAS 16547-4247., 15 pages, 5 figures, 2 appendix figures. Published in ApJ Letters

Background: The muscle quality of the rotator cuff (RC), measured by atrophy and fatty infiltration (FI), is a key determinant of outcomes in RC injury and repair. The ability to regenerate muscle after repair has been shown to be limited. Purpose: To determine if there is a source of resident endogenous stem cells, fibroadipogenic progenitor cells (FAPs), within RC injury patients, and if these cells are capable of adipogenic, fibrogenic, and pro-myogenic differentiation. Study Design: Controlled laboratory study. Methods: A total of 20 patients between the ages of 40 and 75 years with partial- or full-thickness RC tears of the supraspinatus and evidence of atrophy and FI Goutallier grade 1, 2, or 3 were selected from 2 surgeons at an orthopaedic center. During the surgical repair procedure, supraspinatus muscle biopsy specimens were obtained for analysis as were deltoid muscle biopsy specimens to serve as the control. FAPs and satellite cells were quantified using fluorescence-activated cell sorting. Muscle FI and fibrosis was quantified using Oil Red O and Masson trichrome staining. FAP differentiation and gene expression profiles were compared across tear sizes after culture in adipogenic, fibrogenic, and beta-3 agonist (amibegron) conditions. Analysis of variance was used for statistical comparisons between groups, with P < .05 as statistically significant. Results: Histologic analysis confirmed the presence of fat in biopsy specimens from patients with full-thickness tears. There were more FAPs in the full-thickness tear group compared with the partial-thickness tear group (9.43% ± 4.25% vs 3.84% ± 2.54%; P < .01). Full-thickness tears were divided by tear size, with patients with larger tears having significantly more FAPs than those with smaller tears. FAPs from muscles with full-thickness tendon tears had more adipogenic and fibrogenic potential than those with partial tears. Induction of a beige adipose tissue (BAT) phenotype in FAPs was possible, as demonstrated by increased expression of BAT markers and pro-myogenic genes including insulin-like growth factor 1 and follistatin. Conclusion: Endogenous FAPs are present within the RC and likely are the source of FI. These FAPs were increased in muscles with in larger tears but are capable of adopting a pro-myogenic BAT phenotype that could be utilized to improve muscle quality and patient function after RC repair.