151. Inverse correlation between steady-state RNA and cell surface T cell receptor levels.
- Author
-
Maguire JE, McCarthy SA, Singer A, and Singer DS
- Subjects
- Animals, Antigens, Differentiation, T-Lymphocyte analysis, CD4 Antigens analysis, CD8 Antigens, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Organ Specificity, RNA genetics, Receptors, Antigen, T-Cell genetics, Spleen immunology, Thymus Gland immunology, RNA metabolism, Receptors, Antigen, T-Cell biosynthesis, T-Lymphocytes immunology
- Abstract
The relationship between steady-state RNA and cell surface levels of T cell receptor (TCR) was examined in mature T cells and immature CD4+CD8+ double positive thymocytes. TCR is expressed at high levels on the surface of mature T cells and at much lower levels on double positive thymocytes. We demonstrate that in direct contrast to surface expression, TCR-alpha, -beta, CD3-delta, -epsilon, -gamma, and sigma RNA levels are much higher in the immature double positive thymocyte population than in mature T cells. These results demonstrate that quantitative differences in TCR surface expression in immature and mature T cells are not due to increases in TCR RNA levels.
- Published
- 1990
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