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The aim of the research was to investigate the influence of terminal sire line (TSL) and male category (MC) on carcass and meat quality of commercial fatteners. The study was performed on 180 pigs originating from three terminal sire lines: A (Pietrain × Large White), B (pure Pietrain), and C (Pietrain × Duroc × Large White), being assigned to three groups according to MC: immunocastrates (IC, n = 60), surgical castrates (SC, n = 60) and entire males (EM, n = 60). TSL affected most of the carcass and meat quality traits, together with the androstenone concentration. At the same time, MC had a significant effect on fat thickness, ham circumference, drip loss, cooking loss and androstenone and skatole concentrations. A significant interaction effect was observed for carcass length and ham circumference, as well as for most of the measured meat quality traits (except cooking loss, CIE L*, CIE b*, and Warner Bratzler Shear Force (WBSF)). Among the three investigated sire lines, immunocastration was shown to be most beneficial for fatteners sired by the TSL C. However, if deciding to raise EM, fatteners from the TSL B are recommended in terms of carcass and meat quality, although strategies for avoiding boar taint in their carcasses must be taken into consideration.

The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.

Compared to non-megaprojects, megaprojects are often more complex and riskier, and construction employees are thus susceptible to a higher level of psychosocial hazards, which adversely affect their psychosocial health and safety performance. The psychosocial safety climate evaluates the employees’ perceived level of psychosocial health and safety of the workplace and reveals the causes of psychosocial hazards that need to be addressed; it is, therefore, of great significance to determine whether the psychosocial safety climate (PSC) of megaprojects is different from that of non-megaprojects. A questionnaire survey is described involving 10 megaprojects and 143 non-megaprojects in China. The results show that, contrary to expectations, the psychosocial safety climate of megaprojects is significantly better than that of non-megaprojects. Compared with those of non-megaprojects, the employees of megaprojects have a higher common perception of the organization’s emphasis on psychosocial health and safety-related policies, procedures, and behavioral practices in work processes. This research, for the first time, demonstrates and tests the use of the psychosocial safety climate scale (PSC-12) for measuring the construction industry’s PSC, provides insights for understanding the psychosocial safety climate of megaprojects, and serves as a reference for organizational management to intervene in employees’ psychosocial health and correct unsafe behaviors. It also contributes to theoretical research and the measurement standards of psychological safety in megaprojects.

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While natural intoxications with seeds of Ricinus communis (R. communis) have long been known, the toxic protein ricin contained in the seeds is of major concern since it attracts attention of those intending criminal, terroristic and military misuse. In order to harmonize detection capabilities in expert laboratories, an international proficiency test was organized that aimed at identifying good analytical practices (qualitative measurements) and determining a consensus concentration on a highly pure ricin reference material (quantitative measurements). Sample materials included highly pure ricin as well as the related R. communis agglutinin (RCA120) spiked into buffer, milk and meat extract; additionally, an organic fertilizer naturally contaminated with R. communis shred was investigated in the proficiency test. The qualitative results showed that either a suitable combination of immunological, mass spectrometry (MS)-based and functional approaches or sophisticated MS-based approaches alone successfully allowed the detection and identification of ricin in all samples. In terms of quantification, it was possible to determine a consensus concentration of the highly pure ricin reference material. The results provide a basis for further steps in quality assurance and improve biopreparedness in expert laboratories worldwide.

Ricinus communis intoxications have been known for centuries and were attributed to the toxic protein ricin. Due to its toxicity, availability, ease of preparation, and the lack of medical countermeasures, ricin attracted interest as a potential biological warfare agent. While different technologies for ricin analysis have been established, hardly any universally agreed-upon “gold standards” are available. Expert laboratories currently use differently purified in-house materials, making any comparison of accuracy and sensitivity of different methods nearly impossible. Technically challenging is the discrimination of ricin from R. communis agglutinin (RCA120), a less toxic but highly homologous protein also contained in R. communis. Here, we established both highly pure ricin and RCA120 reference materials which were extensively characterized by gel electrophoresis, liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI MS/MS), and matrix-assisted laser desorption ionization–time of flight approaches as well as immunological and functional techniques. Purity reached >97% for ricin and >99% for RCA120. Different isoforms of ricin and RCA120 were identified unambiguously and distinguished by LC-ESI MS/MS. In terms of function, a real-time cytotoxicity assay showed that ricin is approximately 300-fold more toxic than RCA120. The highly pure ricin and RCA120 reference materials were used to conduct an international proficiency test.

The detection and identification of botulinum neurotoxins (BoNT) is complex due to the existence of seven serotypes, derived mosaic toxins and more than 40 subtypes. Expert laboratories currently use different technical approaches to detect, identify and quantify BoNT, but due to the lack of (certified) reference materials, analytical results can hardly be compared. In this study, the six BoNT/A1–F1 prototypes were successfully produced by recombinant techniques, facilitating handling, as well as improving purity, yield, reproducibility and biosafety. All six BoNTs were quantitatively nicked into active di-chain toxins linked by a disulfide bridge. The materials were thoroughly characterized with respect to purity, identity, protein concentration, catalytic and biological activities. For BoNT/A1, B1 and E1, serotypes pathogenic to humans, the catalytic activity and the precise protein concentration were determined by Endopep-mass spectrometry and validated amino acid analysis, respectively. In addition, BoNT/A1, B1, E1 and F1 were successfully detected by immunological assays, unambiguously identified by mass spectrometric-based methods, and their specific activities were assigned by the mouse LD50 bioassay. The potencies of all six BoNT/A1–F1 were quantified by the ex vivo mouse phrenic nerve hemidiaphragm assay, allowing a direct comparison. In conclusion, highly pure recombinant BoNT reference materials were produced, thoroughly characterized and employed as spiking material in a worldwide BoNT proficiency test organized by the EQuATox consortium.

Oxidation is one of the major reasons for impaired quality of meat and meat products but can be prevented by the addition of antioxidants. In the present study, the effect of dietary sweet chestnut wood extract and hop cones on the quality and oxidative stability of meat and dry-cured products was investigated. Control pigs (N = 11) were fed a commercial diet (13.1 MJ metabolizable energy, 15.5% crude protein), while the other two experimental groups were supplemented with 3% of sweet chestnut wood extract (Tannin; N = 12) or 0.4% of hop cones (Hops; N = 11). The quality of meat and dry-cured products was evaluated by means of chemical composition, water holding capacity, objective color, and lipid and protein oxidation. No major effects of sweet chestnut wood extract or of hops supplementation were observed, nevertheless, some indications of improved water holding capacity could be attributed to antioxidants supplementation. The color evolution of dry-cured bellies from Tannin and Hops groups of pigs during refrigerated storage was also indicative of an improved oxidative stability.

This study aimed to develop the method for determination of the ultra-small superparamagnetic iron oxide nanoparticle (USPION)-originated iron (UOI) in the tissues of rats on the basis of the magnetic characteristics (MC) in the liver, left heart ventricle (LHV), kidneys, aorta and blood of Wistar-Kyoto (WKY). Rats were treated intravenously by USPIONs dispersed in saline (transmission electron microscope (TEM) mean size ~30 nm, hydrodynamic size ~51 nm, nominal iron content 1 mg Fe/mL) at the low iron dose of 1 mg/kg. MC in the form of the mass magnetisation (M) versus the magnetic field (H) curves and temperature dependences of M (determined using the SQUID magnetometer), histochemical determination of iron (by Perl’s method) and USPION-induced superoxide production (by lucigenin-enhanced chemiluminescence) were investigated 100 min post-infusion. USPIONs significantly elevated superoxide production in the liver, LHV, kidney and aorta vs. the control group. Histochemical staining confirmed the presence of iron in all solid biological samples, however, this method was not suitable to unequivocally confirm the presence of UOI. We improved the SQUID magnetometric method and sample preparation to allow the determination of UOI by measurements of the MC of the tissues at 300 K in solid and liquid samples. The presence of the UOI was confirmed in all the tissues investigated in USPIONs-treated rats. The greatest levels were found in blood and lower amounts in the aorta, liver, LHV and kidneys. In conclusion, we have improved SQUID-magnetometric method to make it suitable for detection of low amounts of UOI in blood and tissues of rats.

Classification of carcasses at the slaughter line allows an optimisation of its processing and differentiated payment to producers. Boar taint is a quality characteristic that is evaluated in some slaughter plants. This odour and flavour is mostly present in entire males and perceived generally by sensitive consumers as unpleasant. In the present work, the methodologies currently used in slaughter plants for boar taint classification (colorimetric method and sensory quality control-human nose) and the methodologies that have the potential to be implemented on/at the slaughter line (mass spectrometry, Raman and biosensors) have been summarized. Their main characteristics are presented and an analysis of strengths, weaknesses, opportunities and threats (SWOT) has been carried out. From this, we can conclude that, apart from human nose, the technology that arises as very promising and available on the market, and that will probably become a substitute for the colorimetric method, is the tandem between the laser diode thermal desorption ion source and the mass spectrometry (LDTD-MS/MS) with automation of the sampling and sample pre-treatment, because it is able to work at the slaughter line, is fast and robust, and measures both androstenone and skatole.

Due to the strong public initiative in Europe and increased regulator focus to mitigate pain, surgical castration of pigs is being gradually abandoned, while the importance of other sex categories like entire males (EM) and immunocastrates (IC) increases. Although beneficial for animal welfare and economics, their use also brings forward several quality problems. Besides the occurrence of boar taint in EM, these include excessive carcass leanness, softer fat, meat color and pH deviations, inferior water holding capacity and increased meat toughness. In this paper, the raw material differences between the male sex categories and their influence on product quality are reviewed, and possible solutions are presented. Using EM for dried or thermally processed products may result in lower processing yields and inferior sensory quality, which may partially be prevented by applying specific processing adaptations. Immunocastration is a viable solution, especially when prolonging the vaccination to slaughter interval. Low to medium levels of boar taint can be effectively managed in most of the meat products, applying procedures like cooking, microbial inoculation or masking (by spices and especially smoking), while highly tainted material can be valorized only by combining various methods and/or with dilution of the tainted meat.

Surgical castration of piglets without pain relief is still common practice in many countries. Possible alternatives for surgical castration are application of pain relief or anaesthesia or production of boars (entire males) and immunocastrates. Each of these alternatives faces advantages and disadvantages which may result in different citizen attitudes and consumers acceptability. Understanding which practice is acceptable to whom and why may further stimulate implementation. Consumer (n = 3251) and stakeholder (n = 1027) attitudes towards surgical castration without pain relief, surgical castration with anaesthesia, immunocastration, and production of boars were surveyed from April to June 2020 via an online questionnaire in 16 countries (>175 respondents per country). Surgical castration without pain relief was separated from each of the alternatives due to animal welfare and showed the lowest acceptability (32%). Within the alternatives, a further partitioning between the alternatives was based on perceived quality and food safety, with an acceptance of 85% for applying anaesthesia, 71% for immunocastration, and 49% for boar production. Differences depending on professional involvement and familiarity with agriculture could be observed, mainly for the acceptance of surgical castration without anaesthesia, immunocastration, and boars. Castration with anaesthesia was highly accepted by all types of respondents.

Modern pig breeds, which have been genetically improved to achieve fast growth and a lean meat deposition, differ from local pig breeds with respect to fat deposition, fat specific metabolic characteristics and various other properties. The present review aimed to elucidate the mechanisms underlying the differences between fatty local and modern lean pig breeds in adipose tissue deposition and lipid metabolism, taking into consideration morphological, cellular, biochemical, transcriptomic and proteomic perspectives. Compared to modern breeds, local pig breeds accumulate larger amounts of fat, which generally contains more monounsaturated and saturated fatty acids; they exhibit a higher adipocyte size and higher activity of lipogenic enzymes. Studies using transcriptomic and proteomic approaches highlighted several processes like immune response, fatty-acid turn-over, oxidoreductase activity, mitochondrial function, etc. which differ between local and modern pig breeds.

Alternative solutions to the surgical castration of piglets need to be assessed because this is a particularly sensitive issue for the processing of traditional pork products. Currently, the available information about the advantages and drawbacks of castration for dry-cured products is limited; thus, the objective of this study was to evaluate the quality of Slovenian dry-cured ham (Kraški pršut) from entire males (EM), immunocastrates (IC) and surgical castrates (SC). Hams (12 per sex group) were processed for one year and physical-chemical, rheological and sensory analysis of the dry-cured hams was performed. With regard to processing aptitude, the main difference was in the subcutaneous fat thickness, which influenced the level of dehydration and salt intake. This was further reflected in the physical-chemical traits and the texture, which were measured instrumentally or assessed by panelists. Regarding the aforementioned traits, EM and IC were generally similar and different from SC. On the contrary, sensory profiling of odor, taste and flavor demonstrated that EM had the lowest overall sensory quality, different from both IC and SC, and presented odors and flavors described as sweat, manure, sharp and persistent. We confirmed that dry-curing did not eliminate the perception of boar taint in the product from EM. The IC were similar in many aspects to EM except for the odor, taste and flavor of dry-cured hams, in which case they were more similar to SC.

This study investigated the effects of (−)-epicatechin (Epi) in young male borderline hypertensive rats (BHR) during two weeks of treatment (Epi group, 100 mg/kg/day p.o.) and two weeks post treatment (PE group). Epi reduced blood pressure (BP), which persisted for two weeks post treatment. This was associated with delayed reduction of anxiety-like behaviour. Epi significantly increased nitric oxide synthase (NOS) activities in the aorta and left heart ventricle (LHV) vs. the age-matched controls without affecting the brainstem and frontal neocortex. Furthermore, Epi significantly reduced the superoxide production in the aorta and relative content of iron-containing compounds in blood. Two weeks post treatment, the NOS activities and superoxide productions in the heart and aorta did not differ from the age-matched controls. The gene expressions of the NOSs (nNOS, iNOS, eNOS), nuclear factor erythroid 2-related factor 2 (Nrf2), and peroxisome proliferator-activated receptor-γ (PPAR-γ) remained unaltered in the aorta and LHV of the Epi and PE groups. In conclusion, while Epi-induced a decrease of the rats’ BP persisted for two weeks post treatment, continuous Epi treatments seem to be necessary for maintaining elevated NO production as well as redox balance in the heart and aorta without changes in the NOSs, Nrf2, and PPAR-γ gene expressions.

Construction cost and duration are two critical project indicators. It is acknowledged that these two indicators are closely dependent and highly uncertain due to various common factors and limited data for explanatory model calibration. However, the stochastic dependence underlying construction cost and duration is usually ignored and the subsequent probabilistic analysis can be misleading. In response, this study develops a Nataf distribution model of building cost and duration, in which the uncertainties of total cost, unit cost, and duration are respectively quantified by univariate distribution fitting, while their stochastic dependence is inferred by maximum likelihood estimation. This method is applied to the costs and durations of 77 China residential building projects completed between 2011 and 2016. The goodness of fit test illustrates that the data conform well to the developed model. The conditional distributions of cost and duration are then derived and the corresponding conditional expectations and variances are given. The results provide the distribution of building costs for a desired duration and the expected duration given a budget. This, together with the ability to update probabilities when new project information is available, confirms the potential of the proposed model to benefit precontract decision making from a risk perspective.

Abstract Time‐series models applied in the study of animal population dynamics commonly assume linearity on the log‐scale, leading to log‐normally distributed rates of increase. While this is often computationally convenient, in particular when performing statistical inference in the presence of observation error, it may lead to unrealistic predictions for animals with a limited reproduction. We introduce a model that includes an explicit bound on the reproductive rate of an individual, and apply this to a population time series of ungulates in Kruger National Park, South Africa. Due to observational error, the year‐to‐year increases in animal counts occasionally exceeded the maximal reproductive rate of the animals. In such cases, the traditional unbounded model showed a tendency of overfitting data, leading to unrealistic predictions of the underlying population increase. An observed increase above the maximal reproductive rate also provides empirical confirmation that observation error exists. The model with an explicit bound was able to utilize this in order to separate observational error from population process noise, which the traditional unbounded model was unable to do. We conclude that enforcing a strict upper bound on the reproductive rate of an animal population model may lead to more realistic statistical inference than commonly applied log‐linear models when an explicit bound on reproductive rate is known. We further conclude that introducing a bound on reproduction can greatly assist in separating observational error and population process noise for slow life histories, or more generally, when the rate of sampling is high compared to reproductive rates.

Consumers frequently make decisions about how much they are willing to pay (WTP) for specific products and services, but little is known about the neural mechanisms underlying such calculations. In this study, we were interested in testing whether specific brain activation—the asymmetry in engagement of the prefrontal cortex—would be related to consumer choice. Subjects saw products and subsequently decided how much they were willing to pay for each product, while undergoing neuroimaging using electroencephalography. Our results demonstrate that prefrontal asymmetry in the gamma frequency band, and a trend in the beta frequency band that was recorded during product viewing was significantly related to subsequent WTP responses. Frontal asymmetry in the alpha band was not related to WTP decisions. Besides suggesting separate neuropsychological mechanisms of consumer choice, we find that one specific measure—the prefrontal gamma asymmetry—was most strongly related to WTP responses, and was most coupled to the actual decision phase. These findings are discussed in light of the psychology of WTP calculations, and in relation to the recent emergence of consumer neuroscience and neuromarketing.

Deciding whether bid for the construction project is classic risk-based decision-making that is crucial to the survival and prosperousness of construction companies. More contractors are adopting group bidding decision-making to reduce the number of incorrect decisions. However, previous related studies mainly focus on project factors that overlook the members of bidding groups and the interaction between bidding group members. The aim of this paper, therefore, is to investigate the potential factors that influence group bidding decision-making for construction projects. Twenty factors relating to individual, group, and organizational levels are identified through a literature review and interviews with experienced practitioners. A questionnaire survey of 203 Chinese international contractors is conducted to obtain the importance of the factors involved. The results of the ranking analysis indicate 14 critical factors, among which “risk perception” and “team decision preference” are regarded as the two most significant. Moreover, 20 factors can be classified into five groupings: (1) values and strategic goals, (2) collaboration and learning orientation, (3) consensus reaching, (4) risk awareness, and (5) empowerment and development, with risk awareness having an especially strong influence. This research deepens the understanding of factors belonging to different organizational levels that need to be highlighted during the group bidding decision-making process and provides strategies for bidding groups and their companies to improve their decision performance.

Waste-to-energy (WTE) incineration technologies are considered an effective solution for sustainable and efficient municipal solid waste (MSW) disposal in China, and the public-private partnership (PPP) arrangement has been widely used to construct and operate WTE incineration projects. However, PPP WTE incineration projects in China are affected by numerous risks due to the long concession period, various participants, and other factors commonly involved in PPPs, resulting in a number of failures. In light of the pivotal role that risk identification, analysis, and response play in the successful development of PPP WTE incineration projects, this paper presents a multiple case study to identify the risk factors involved in China by drawing on experience from the real-life risk events of 35 PPP WTE incineration plants. 18 risk factors are identified; the most critical of which being public opposition risk, environmental pollution risk, government decision-making risk, a defective legal and regulatory system, and MSW supply risk. The results of the study provide a solid foundation for the future risk analysis, risk allocation, and risk response of PPP WTE incineration projects, and shed light on performance improvement of the PPP WTE incineration projects as well as the development of the PPP WTE industry in China.

The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje). We successfully analyzed allelic variation in 39 polymorphisms, located in 33 candidate genes. Results provide relevant information regarding genetic diversity and segregation of SNPs associated to production and quality traits. Coat color and morphological trait-genes that show low level of segregation, and fixed SNPs may be useful for traceability. On the other hand, we detected SNPs which may be useful for association studies as well as breeding programs. For instance, we observed predominance of alleles that might be unfavorable for disease resistance and boar taint in most breeds and segregation of many alleles involved in meat quality, fatness and growth traits. Overall, these findings provide a detailed catalogue of segregating candidate SNPs in 20 European local pig breeds that may be useful for traceability purposes, for association studies and for breeding schemes. Population genetic analyses based on these candidate genes are able to uncover some clues regarding the hidden genetic substructure of these populations, as the extreme genetic closeness between Iberian and Alentejana breeds and an uneven admixture of the breeds studied. The results are in agreement with available knowledge regarding breed history and management, although largest panels of neutral markers should be employed to get a deeper understanding of the population's structure and relationships.

Isolated dislocations of the scaphoid are extremely uncommon injuries and are often associated with significant ligamentous failures. Since scaphoid dislocations typically present with associated carpal fractures, few cases of isolated dislocations of the scaphoid exist in the literature. The proposed treatment options in the literature range from closed reduction and casting to open reduction and internal fixation. We present the case of a 41-year-old male with an isolated scaphoid dislocation in whom open reduction and internal fixation was performed with K-wires. At five months follow-up, the patient had returned to work and all desired activities.

The effect of dietary fibre content on intestinal production of indolic compounds was studied in immunocastrated pigs (IC). In addition, entire males (EM) and IC were compared on control diet (with low fibre content). For the study 32 crossbred pigs were assigned, within a litter to 4 treatment groups; 24 pigs were immunocastrated (at the age of 77 and 112 days) and 8 pigs were kept as entire males (EM). IC were split into three groups (IC_H, IC_M and IC_L) fed three diets differing in crude fibre (34, 60 and 80 g/kg dry matter, respectively) and net energy (NE) (10.0, 9.3, 8.5 MJ NE/kg/DM, respectively). EM were fed high NE i.e. low fibre diet. The experiment started when pigs were 84 days old and finished at the age of 172 days, when pigs were sent to slaughter. Skatole and indole concentrations were determined in the samples of intestinal content taken from caecum (CE), ascending (AC) and descending colon (DC). The concentration of indole was the highest in CE and proximal part of the colon, while skatole concentration increased in the distal parts of the large intestine. Concentrations of indolic compounds did not differ between EM and IC that were fed the same diet. Lowering dietary NE by inclusion of high fibre ingredients reduced the production of indole in the intestinal content of IC pigs, whereas the production of skatole was not affected.

The article analyses the Host Nation Support (HNS) planning process in five separate phases according to the NATO procedures. The author emphasizes the importance, purpose and result of every planning stage. The main objective of the article is to apply general rules to the concrete examples of international air forces exercises. The author further identifies his comprehension of HNS formed in the specific conditions of the 22th Helicopter Base. The logistic planning process analysis is based on the experience from exercises Ramstein Rover and Ample Strike held in the period of 2012-2014. The paper is focused on exercises preparatory stages that create the basis for a successful accomplishment of the executive part. The results of the article present the trend of planning responsibility delegation to the tactical level and define the specifics of adapted planning process. The author’s contribution may be used as a lead for the future application of the HNS concept.

The paper describes basic principles of ad-hoc routes assignment. Each carrier has its own interface called information system KADR with SŽDC for capacity requests management. The paper describes all steps which must be done before train departure.

Background: The primary aim of the study was to investigate the cytokine/chemokine response in the kidney, lung, and liver following acute kidney injury (AKI). The secondary aim was to test whether α-melanocyte-stimulating hormone (α-MSH) could prevent a reduction in organ function, and attenuate the inflammatory cytokine/chemokine response within the kidney, lung, and liver following AKI in rats with or without preexisting chronic kidney disease (CKD). Methods: A two-stage animal model, in which AKI was induced in rats with preexisting CKD, induced by 5/6 nephrectomy (Nx), was used. Six weeks later, AKI was induced by intestinal ischemia and reperfusion (IIR). Sham procedures [S(Nx) and S(IIR)] were also performed. Results: Increasing levels of serum creatinine (sCr) demonstrated progressive development of CKD in response to Nx, and following IIR sCr levels increased further significantly, except in the S(Nx) group treated with α-MSH. However, no significant differences in the fractional increase in sCr were observed between any of the groups exposed to IIR. In kidney, lung, and liver tissue the levels of interleukin (IL)-1β were significantly higher in rats undergoing IIR when compared to the S(IIR) and control rats. The same pattern was observed for the chemokine monocyte chemoattractant protein (MCP)-1 in lung and liver tissue. Furthermore, kidney IL-1β and RANTES levels were significantly increased after IIR in the Nx rats compared to the S(Nx) rats. Conclusion: Both the functional parameters and the cytokine/chemokine response are as dramatic when AKI is superimposed onto CKD as onto non-CKD. No convincing protective effect of α-MSH was detected.

This paper presents the first inclusive scientometric review of the economic areas of green building (GBE). The aim is to methodically examine and summarize the state-of-the-art of the GBE body of knowledge. To this end, this study analyses 1713 GBE-related bibliographic records retrieved from the Web of Science by using the quantitative method of knowledge mapping. The knowledge base, knowledge domain, and knowledge evolution of how they interacted with each other are explored using document co-citation analysis and keywords co-citation analysis of the existing body of literature. The research findings are informative in recognizing and interpreting the underlying structure and trends in GBE. A knowledge map provides a valuable and instructive understanding of the evolution and status quo of the GBE knowledge body, as well as assisting in recognizing the gaps and deficiencies involved. The results will help in understanding how GBE knowledge is evolving and its role played in green building, and thus provide suggestions of how academic research can enhance sustainability practices in terms of economic area in the future.

Abandoning of male piglets castration in the European Union is a challenge for the pork production sector in particular for high-quality dry-cured traditional products. The information on consumer acceptability of dry-cured products from alternatives is limited, so the objective was to test the consumer acceptability of unsmoked traditional dry-cured belly (Kraška panceta) processed from three sex categories, i.e., surgical castrates (SC), entire males (EM) and immunocastrates (IC). Consumers (n = 331) were asked to taste dry-cured bellies from EM, IC and SC and to score the taste appreciation on a 9 cm unstructured scale. After tasting the pancetta of three sex categories, the consumers attributed the lowest acceptability scores to SC, whereas IC and EM received similar scores. Only about a quarter of consumers attributed the lowest score to EM, mainly when boar taint compounds were present. The results of this study indicate that a certain share of consumers was sensitive to taste deficiencies and that the leanness of this product is very important for consumers.

There are indications of reduced meat quality in entire male pigs (EMs) in comparison to surgically castrated pigs (SCs); however, the differences are not strongly confirmed, and the etiology is not clarified. In the present study, samples of the longissimus dorsi, pars lumborum muscle (LL) from EMs (n = 12) and SCs (n = 12) of the same age and weight were evaluated at the physico-chemical and proteomic level. EMs exhibited lower intramuscular fat content, higher collagen content with higher solubility, a higher level of protein carbonyl groups (indicating higher protein oxidation), lower water holding capacity, and tougher meat than SCs. Proteomic analysis revealed differences in heat shock proteins expression, while a greater abundance of several other identified proteins (malate dehydrogenase, Na/K-transporting adenosintriphosphatase (ATP-ase) subunit alpha-1, and blood plasma proteins) indicates that EMs have a more oxidative metabolic profile than that of SCs. More abundant protein fragments (mainly actin and myosin heavy chain) suggest a higher degree of proteolysis in EMs, which was not followed by lower meat toughness.

Dry-fermented sausages were produced in a traditional way, without addition of nitrites and starter cultures, from meat of an autochthonous breed (Krškopolje pig) raised either in a conventional indoor or organic husbandry system. Physicochemical and sensory analyses were performed at the end of processing to characterize their quality. Dry-fermented sausages from organic pork retained more moisture, which resulted in higher water activity and softer texture (instrumental and sensory). They were more oxidized (higher thiobarbituric acid reactive substances (TBARS)), in agreement with more unsaturated fatty acid profile, a higher score for rancid taste, and a higher relative abundance of volatiles from lipid β-oxidation. Overall, dry-fermented sausages from organic pork had lower levels of volatile compounds, particularly, those originating from spices (despite the same quantity added) and lower levels of amino-acid degradation. Sensory analysis showed that dry-fermented sausages from organic pork had less intensive and vivid color, tasted more bitter and sour, and had more off-tastes. The observed differences could be related to initial differences in raw material (differences in meat pH and level of polyunsaturated fatty acids) affecting the process of fermentation.

In this paper, the notion of stability is extended to network flows over time. As a useful device in our proofs, we present an elegant preflow-push variant of the Gale-Shapley algorithm that operates directly on the given network and computes stable flows in pseudo-polynomial time, both in the static flow and the flow over time case. We show periodical properties of stable flows over time on networks with an infinite time horizon. Finally, we discuss the influence of storage at vertices, with different results depending on the priority of the corresponding holdover edges.

Capitalising useful knowledge for construction procurementselection (CPS) decisions would provide a valuable asset to clientorganisations, as the successful/unsuccessful experience wouldhelp decision-makers avoid the occurrence of similar errors andensure the most suitable procurement system is employed fora construction project. As a result, there is a need to examinethe potential for developing a knowledge management model tocapture and reuse experiential knowledge to guide CPS decisions.This paper begins by identifying a suitable approach for managingCPS knowledge. This is followed by a discussion of the knowledgerequired for CPS decision support. A prototype knowledgemanagementmodel is developed, using the case-based reasoning(CBR) approach, and a mechanism for the retrieval and reuse ofknowledge for guiding CPS decisions is elucidated. The resultsindicate that CBR is a suitable tool for formulating the procurementselection parameters and selecting a suitable procurementsystem for a construction project. This is primarily becausethe CBR approach is flexible enough to allow closely matchinghistoric cases to be retrieved as well as enabling the decisionmakerto adapt the proposed solution based on the predominantcharacteristics of the client, project and external environmentpertinent to the current project.

Despite its obvious theoretical benefits, there has been some reservations regarding the practical implementation of Value Management (VM) for construction projects. In particular, these concerns the extra time and costs involved in conducting VM in relation to the actual benefits gained as a consequence of its use. This paper provides the result of an interview survey of the experiences and observations of 17 professionals working in the property and construction industry to establish the extent to which this is an issue. The main conclusion is that the process is working well, with VM being popular among those with experience in its use - which has been extended into the area of consultant selection. However, in contrast with the prescriptive literature, much of the participants' experiences appear to be more concerned with VM's contribution to the identification and management of the risks involved in project delivery than the straight value-for-money aspects. This may be due to the higher levels of uncertainty involved in construction work than in VM's original use in construction.

This paper provides the results of an exploratory survey of construction industry managersin Singapore to isolate some of the common effects of national and organisational culture,together with the personal characteristics of managers, on the efficacy of project communication.By examination of significant correlation coefficients, the various types of influencesare identified. The results of the research suggest that the managers’ attitude andbehaviours toward communication may be guided to large extent by their level of competence.The study also provides evidence to suggest that the individuals’ understanding ofthe communication process and its barriers, the way they behave with other individualsand expect to be treated, varies according to national cultures.

Postmoderna, to není pouze dekonstrukce, kterou nabízí Jacques Derrida, a která je v českém právním prostředí chápána jako pouhá destrukce. Tento článek se zaměří spíše než na dekonstruktivistické tázání na jazykovou a narativní povahu práva. Cílem je poukázat na proměnu vědění, kterou se současným pojetím práva můžeme spojit: právo nelze poznávat jako konečnou sadu výpovědí o společensky aprobovaném jednání, či jednání, o kterém tvrdíme, že je žádoucí. Je to sada příběhů, která se neustále mění, která se vyvíjí, a ve které se odehrávají impulsivní i plánované boje o ovládnutí významů. Právo není možné poznávat ve své úplnosti. Právo jako celek je pouze ideální typ (Weber), jehož skutečné poznání je nespojité a v úplnosti nemožné. To je právo, na které nám ukazuje postmoderna, a které ukážeme v tomto článku.

Governments usually guarantee the amount of investment income to private sector partners to encourage their participation in Public-Private Partnership urban infrastructure development projects, with the ‘float return on investment guarantee’ being the main method in use by the Chinese government today. The current problems with the float return on investment guarantee are analysed and a guarantee approach with embedded motivatio nal behaviour is presented as an alternative. A pricing method option is then introduced as the motivation-embedded return guarantee has similar charac­teristics to real options. From this, a valuation model is developed that provides the basis of a new systematic method for calculating the government guarantee value.

Recenzní poznámky k textu v tomto čísle Envigogiky: Svobodová, Z. (2016). O pasivitě člověka ke světu. Envigogika, 11(1). DOI: http://dx.doi.org/10.14712/18023061.517

In the winter of 1464, the Swedish bishop Kettil Karlsson (Vasa) instigated an uprising against King Christian I of Oldenburg who at the time ruled all of Scandinavia. In order to crush the uprising the king quickly responded with a war campaign against the insurgents. In April his army was ambushed in Hälla forest in Västmanland by forces commanded by the bishop. In the battle that followed the king was defeated and fled back to Stockholm. The study of medieval warfare in Sweden has hitherto yielded little interest among scholars. The aim of this article is thus to add to this sub-ject by investigating these events from a military point of view. The article address the question of the actual geographical location of the battle, what elements the two clash-ing armies composed of, further, what really happened during the course of the battle and finally how the battle ended and what the military consequences were.

Med stigende fokus på øget tværfaglighed og digitalisering i undervisningen på Københavns Universitet undersøger vi i denne artikel hvilke betingelser, der skal til, for at tværfaglighed og digitalisering rent faktisk kan omsættes til læringsudbytte? Det gør vi ved at reflektere over hvilke didaktiske komponenter, der har været virksomme i et nyligt afholdt, tværfagligt kursus: KOrtlægning af VIdenskabelige KOntroverser (KOVIKO). Vi identificerer tre vigtige komponenter som afgørende for de opnåede læringsmæssige resultater: Socialisering til et nyt fagligt fællesskab; internettet som omdrejningspunkt og oplæring til tværfaglige læringsindsatser. I konklusionen fremhæver vi det potentiale, som ligger i brug af digitale metoder og platforme som et fagligt integrerende element i "undervisningsbaseret forskning".With an increasing focus on interdisciplinarity and digitalization in teaching at the University of Copenhagen, we use this article to examine some of the conditions under which interdisciplinarity and digitalization can lead to better learning outcomes as well as improved study environments for students. We do this by reflecting on which didactic components were effective in a recently held course on Mapping Scientific Controversies (KOVIKO). We identify three components as particularly important factors behind achieved learning outcomes and improvements in study environment: an active effort to socialize students into a new interdisciplinary community; the internet as the pivotal object of the course; and encouraging interdisciplinary learning. We conclude by highlighting the potential that digital methods, as integrated elements of “teaching-based research”, hold.

Xanthomonas campestris is a phytopathogenic bacterium and causes many diseases of agricultural relevance. Volatiles were shown to be important in inter- and intraorganismic attraction and defense reactions. Recently it became apparent that also bacteria emit a plethora of volatiles, which influence other organisms such as invertebrates, plants and fungi. As a first step to study volatile-based bacterial–plant interactions, the emission profile of Xanthomonas c. pv. vesicatoria 85-10 was determined by using GC/MS and PTR–MS techniques. More than 50 compounds were emitted by this species, the majority comprising ketones and methylketones. The structure of the dominant compound, 10-methylundecan-2-one, was assigned on the basis of its analytical data, obtained by GC/MS and verified by comparison of these data with those of a synthetic reference sample. Application of commercially available decan-2-one, undecan-2-one, dodecan-2-one, and the newly synthesized 10-methylundecan-2-one in bi-partite Petri dish bioassays revealed growth promotions in low quantities (0.01 to 10 μmol), whereas decan-2-one at 100 μmol caused growth inhibitions of the fungus Rhizoctonia solani. Volatile emission profiles of the bacteria were different for growth on media (nutrient broth) with or without glucose.

Real estate developers in China are using mergers and acquisitions (M&As) to ensure their survival and competitiveness. However, no suitable method is yet available to assess whether such M&As provide enhanced value for those involved. Using a hybrid method of data envelopment analysis (DEA) and Malmquist total factor productivity (TFP) indices, this paper evaluates the short and medium term effects of M&As on acquirers’ economic performance with a set of 32 M&A cases occurring during 2000–2011 in China. The results of the analysis show that M&As generally have a positive effect on acquirers’ economic performance. Acquisitions on average experienced a steady growth in developer Malmquist TFP, a more progressive adoption of technology immediately after acquisition, a slight short-term decrease in technical efficiency after acquisition but followed by a marked increase in the longer term once the integration and synergy benefits were realised. However, there is no evidence to show whether developers achieved any short or long term scale efficiency improvements after M&A. The findings of this study provide useful insights on developer M&A performance from an efficiency and productivity perspective.