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Compared with natural enzymes, artificial mimic enzymes have been widely studied for their high stability and cost effectiveness. In this study, CuSe nanoplates as a simulated enzyme which does not contain precious metals, has peroxidase activity. CuSe nanoplates were prepared and characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray energy dispersive spectrometer (EDS). Kinetic studies show that CuSe nanoplates exhibits a higher affinity for 3,3′,5,5′-teramethylbenzidine (TMB) than horseradish peroxidase (HRP). The rapid colorimetric determination of H2O2 and L-cysteine were developed based on the catalytic efficiency. The linear range of detection for H2O2 is 5.0×10−6~8.0×10−5 M, and the detection limit is 2.9×10−6 M, while the relative standard error is less than 5%. In addition, L-cysteine was detected with a detection limit of 0.2×10−6 M. The good selectivity of the determination to H2O2 and L-cysteine in aqueous solution was also achieved. CuSe nanoplates as a simulated enzyme for sensor applications would be used in environmental monitoring and biomedical analysis.

Additional file 9: Supplementary Fig S7. Co-transformation of VaPYL9 and VaPCMT. VaPYL9 and VaPCMT were together introduced into Y2H gold and its presence was identified in Y2H gold by 1 % agarose gel electrophoresis.

Additional file 1: Supplementary Table S1. Detailed bioinformatic information of VaPYL9 gene in grape.

Additional file 4: Supplementary Fig S2. The construction of VaPYL9 transient expression vector. The presence of VaPYL9 were verified including amplified cDNA (a), introduced into Escherichia coalition (b) and Agrobacterium tumefaciens strain GV3101 (c) by 1 % agarose gel eletrophoresis, respectively.

Additional file 8: Supplementary Fig S6. Self-activation toxicity test of bait protein VaPYL9 and prey protein VaPCMT. Dental plaque didn’t show blue. They were verified that there were no self-activation toxicity.

Additional file 6: Supplementary Fig S4. The identification of transgenic tomatoes. Through Agrobacterium-mediated leaf disk transformation method, transgenic tomatoes were gained. Through screening, stable and homozygous lines (#2, #6, #9) were identified and named OE1, OE2, OE3 by 1 % agarose gel electrophoresis. These stable lines were used for the following experiment.

Additional file 3: Supplementary Fig S1. Multiply sequence alignment of PYL gene members among grape, tomato, Arabidopsis thaliana. Underline part was conserved domain (polyketide-cyc2) feature in PYL sequences. The length of VaPYL9 domain sequence was 1-178.

Additional file 5: Supplementary Fig S3. The construct of VaPYL9 overexpression vector. The presence of VaPYL9 were verified including amplified cDNA (a), introduced into Escherichia coalition (b) and Agrobacterium tumefaciens strain GV3101 (c) by 1 % agarose gel eletrophoresis, respectively.

Additional file 7: Supplementary Fig S5. The construction pGBKT7-VaPYL9 and pGADT7-VaPCMT. The presence of VaPYL9 (a) and VaPCMT (b) were verified including amplified cDNA, introduced into Y2H gold by 1 % agarose gel electrophoresis, respectively.

Cysteine-rich receptor-like kinases (CRKs) took crucial roles in plant cell growth and development, as well as environmental adaption. Apple (Malus domestica) had been considered a staple fruit crop and a model of woody plants. In this study, the annotation, evolution, and duplication of the CRK gene family members in apple (MdCRK) were characterized. Besides, we also investigated the expressional patterns of MdCRKs in various tissue types and response to signals from Alternaria alternate apple pathotype (AaAP), Penicillium expansum (Pe), and Valsa mali (Vm). A total of 36 MdCRKs were annotated. The phylogenetic analysis of CRKs among apple, Arabidopsis thaliana, rice (Oryza sativa), cotton (Gossypium hirsutum), banana (Musa acuminata), and tomato (Solanum lycopersicum) revealed the distinct evolutionary characteristics in plants. Based on gene duplication analysis, seven tandem gene clusters containing 24 members and ten segmental gene pairs were found from MdCRKs. A large number segmental gene pairs were identified from CRKs between apple and cotton. Abscisic acid (ABA) and methyl jasmonate (MeJA)–responsive cis-elements were discovered from the promoter region of most MdCRKs. MdCRKs showed distinct tissue and developmental expression patterns. Most members displayed distinct expression patterns among various tissue types. Six members were differentially expressed in response to signals from at least two pathogenic fungi. Our study provides valuable information for further studies on the evolution and functional investigation on disease resistance of CRKs.

To observe the effect of electroacupuncture (EA) on the expression of soluble guanylate cyclase (sGC), cyclic guanosine phosphate (cGMP) and protein kinase G (PKG) of cerebral vascular smooth muscle in cerebral infarction (CI) rats, so as to study its dynamic regulation mechanism.Male Wistar rats were randomly divided into normal control (After modeling, the immunoactivity and activities of sGC at 3 h, PKG at 3 and 6 h and cGMP from 3 h to 24 h were ob-viously decreased in the model group relevant to the normal control and sham-operation groups (EA intervention can significantly inhibit the down-regulation of sGC, PKG and cGMP expression of cerebral artery smooth muscle in MCAO model rats, which plays an important role in inhibiting cerebral artery smooth muscle spasm after ischemia, maintaining normal vascular function and state, and thus increasing blood perfusion around cerebral infarction area. However, acupuncture effect has a certain time-effectiveness.

Additional file 2: Supplementary Table S2. Information of the BAM genes used to construct the phylogenetic tree in Fig. 1a.

Additional file 1: Supplementary Table S1. qRT-PCR primers for expression on analysis of VvBAM genes.

Additional file 3: Supplementary Table S3. Summary of reads based on RNA sequence data obtained from each sample after 24 h LT stress.