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576 results on '"Maelandsmo, Gunhild M"'

152. Species-specific in vivo engraftment of the human BL melanoma cell line results in an invasive dedifferentiated phenotype not present in xenografts

Author

Cedervall, Jessica, Jamil, Seema, Prasmickaite, Lina, Cheng, YenFu, Eskandarpour, Malihe, Hansson, Johan, Maelandsmo, Gunhild M., Ringborg, Ulrik, Gulyas, Miklos, Zhen, He Suo, Kanter, Lena, Ahrlund-Richter, Lars, Cedervall, Jessica, Jamil, Seema, Prasmickaite, Lina, Cheng, YenFu, Eskandarpour, Malihe, Hansson, Johan, Maelandsmo, Gunhild M., Ringborg, Ulrik, Gulyas, Miklos, Zhen, He Suo, Kanter, Lena, and Ahrlund-Richter, Lars

Abstract

For clinically relevant studies on melanoma progression and invasiveness, in vivo experimental systems with a human cellular microenvironment would be advantageous. We have compared tumor formation from a human cutaneous malignant melanoma cell line (BL), after injection as conventional xenografts in the mouse, or when injected into a predominantly species-specific environment of human embryonic stem cell-derived teratoma induced in the mouse (the hEST model). The resulting melanoma histology was generally analogous, both systems showing delimited densely packed areas with pleomorphic cells of malignant appearance. A specificity of the integration process into the human embryonic teratoma tissues was indicated by the melanoma exclusively being found in areas compatible with condensed mesenchyme, similar to neural crest development. Here, also enhanced neovascularization was seen within the human mesenchymal tissues facing the BL melanoma growth. Furthermore, in the hEST model an additional melanoma cell phenotype occurred, located at the border of, or infiltrating into, the surrounding human loose mesenchymal fibrous stroma. This BL population had a desmoplastic spindle-like appearance, with markers indicative of dedifferentiation and migration. The appearance of this apparently more aggressive phenotype, as well as the induction of human angiogenesis, shows specific interactions with the human embryonic microenvironment in the hEST model. In conclusion, these data provide exciting options for using the hEST model in molecular in vivo studies on differentiation, invasiveness, and malignancy of human melanoma, while analyzing species-specific reactions in vivo.

Published
2009
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153. Protein Expression of the Cell-Cycle Inhibitor p27Kip1 in Malignant Melanoma : Inverse Correlation with Disease-Free Survival

Author

Flørenes, Vivi Ann, Mælandsmo, Gunhild M., Kerbel, Robert S., Slingerland, Joyce M., Nesland, Jahn M., and Holm, Ruth

Subjects
Cyclin-Dependent Kinase Inhibitor p21, Skin Neoplasms, Tumor Suppressor Proteins, Cell Cycle Proteins, Cyclin-Dependent Kinases, Disease-Free Survival, Immunoenzyme Techniques, Survival Rate, Cyclins, Humans, Neoplasm Invasiveness, Enzyme Inhibitors, Neoplasm Metastasis, Melanoma, Microtubule-Associated Proteins, Nevus, Cyclin-Dependent Kinase Inhibitor p27, Regular Articles
Abstract

In the present study we analyzed, by immunohistochemistry, a panel of human melanomas for protein expression of the cyclin-dependent kinase (cdk) inhibitor p27Kip1 and evaluated whether deregulated expression correlates with clinical outcome for this type of cancer. We found that p27Kip1 was strongly expressed by normal melanocytes and benign nevi, whereas in malignant melanoma, a heterogeneous expression pattern was observed. In the case of nodular melanomas, the level of p27Kip1 was found to correlate significantly with the thickness of the tumor, with less protein expressed in thicker lesions. We also found that patients having tumors with fewer than 5% p27Kip1-staining cells had a significantly higher risk of early relapse of their disease compared with those expressing moderate or high levels. In contrast, the level of p27Kip1 did not correlate with tumor thickness or disease-free survival in patients with superficial spreading melanomas, suggesting that p27Kip1 may play different roles in these two major pathological subgroups of malignant melanoma. Furthermore, p27Kip1 did not appear to have an influence on overall survival for either subgroup. When we examined the combined effect of p21WAF1/CIP1 (another cdk inhibitor) and p27Kip1 on clinical outcome, we found that analysis of these two cdk inhibitors together may have greater prognostic potential than either alone. In conclusion, our results suggest that virtually complete loss of p27Kip1 protein expression has potential importance as a prognostic indicator of early relapse in patients with nodular melanoma. The results, furthermore, underscore the value of analyzing multiple cell cycle regulatory proteins to obtain the most reliable indication of prognosis.

Published
1998

154. Osteopontin is a prognostic biomarker in non-small cell lung cancer

Author

Rud, Ane Kongsgaard, primary, Boye, Kjetil, additional, Øijordsbakken, Miriam, additional, Lund-Iversen, Marius, additional, Halvorsen, Ann Rita, additional, Solberg, Steinar K, additional, Berge, Gisle, additional, Helland, Åslaug, additional, Brustugun, Odd Terje, additional, and Mælandsmo, Gunhild M, additional

Published
2013
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160. Nuclear Legumain Activity in Colorectal Cancer

Author

Haugen, Mads H., primary, Johansen, Harald T., additional, Pettersen, Solveig J., additional, Solberg, Rigmor, additional, Brix, Klaudia, additional, Flatmark, Kjersti, additional, and Maelandsmo, Gunhild M., additional

Published
2013
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161. Expression of S100A4 by a variety of cell types present in the tumor microenvironment of human breast cancer

Author

Cabezón, Teresa, Celis, Julio E, Skibshøj, Inge, Klingelhöfer, Jörg, Grigorian, Mariam, Gromov, Pavel, Rank, Fritz, Myklebust, June Helen, Maelandsmo, Gunhild M, Lukanidin, Eugene, Ambartsumian, Noona, Cabezón, Teresa, Celis, Julio E, Skibshøj, Inge, Klingelhöfer, Jörg, Grigorian, Mariam, Gromov, Pavel, Rank, Fritz, Myklebust, June Helen, Maelandsmo, Gunhild M, Lukanidin, Eugene, and Ambartsumian, Noona

Abstract

The S100A4 protein, which is involved in the metastasis process, is a member of the S100 superfamily of Ca-binding proteins. Members of this family are multifunctional signaling proteins with dual extra and intracellular functions involved in the regulation of diverse cellular processes. Several studies have established a correlation between S100A4 protein expression and worse prognosis for patients with various malignancies including breast cancer. In this article, we have used specific antibodies in combination with immunohistochemistry (IHC) to identify the cell types that express S100A4 in human breast cancer biopsies obtained from high-risk patients. IHC analysis of 68 tumor biopsies showed that the protein is expressed preferentially by various cell types present in the tumor microenvironment (macrophages, fibroblasts, activated lymphocytes), rather than by the tumor cells themselves. Moreover, we show that the protein is externalized by the stroma cells to the fluid that bathes the tumor microenvironment, where it is found in several forms that most likely correspond to charge variants. Using a specific ELISA test, we detected a significant higher concentration of S100A4 in the tumor interstitial fluid (TIF) as compared to their corresponding normal counterparts (NIF).

Published
2007

166. In vivo MRI and histopathological assessment of tumor microenvironment in luminal-like and basal-like breast cancer xenografts

Author

Huuse, Else M., primary, Moestue, Siver A., additional, Lindholm, Evita M., additional, Bathen, Tone F., additional, Nalwoga, Hawa, additional, Krüger, Kristi, additional, Bofin, Anna, additional, Maelandsmo, Gunhild M., additional, Akslen, Lars A., additional, Engebraaten, Olav, additional, and Gribbestad, Ingrid S., additional

Published
2011
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183. Species-Specific In vivo Engraftment of the Human BL Melanoma Cell Line Results in an Invasive Dedifferentiated Phenotype Not Present in Xenografts

Author

Cedervall, Jessica, primary, Jamil, Seema, additional, Prasmickaite, Lina, additional, Cheng, YenFu, additional, Eskandarpour, Malihe, additional, Hansson, Johan, additional, Mælandsmo, Gunhild M., additional, Ringborg, Ulrik, additional, Gulyas, Miklos, additional, Zhen, He Suo, additional, Kanter, Lena, additional, and Ährlund-Richter, Lars, additional

Published
2009
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190. Expression of S100A4 by a variety of cell types present in the tumor microenvironment of human breast cancer

Author

Cabezón, Teresa, primary, Celis, Julio E., additional, Skibshøj, Inge, additional, Klingelhöfer, Jörg, additional, Grigorian, Mariam, additional, Gromov, Pavel, additional, Rank, Fritz, additional, Myklebust, June Helen, additional, Mælandsmo, Gunhild M., additional, Lukanidin, Eugene, additional, and Ambartsumian, Noona, additional

Published
2007
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192. Poly(sebacic anhydride) nanocapsules as carriers: effects of preparation parameters on properties and release of doxorubicin.

Author

Bagherifam, Shahla, Griffiths, Gareth W., Mælandsmo, Gunhild M., Nyström, Bo, Hasirci, Vasif, and Hasirci, Nesrin

Subjects
ANHYDRIDES, DRUG delivery systems, DOXORUBICIN, NANOCAPSULES, NANOBIOTECHNOLOGY
Abstract

Poly(sebacic anhydride) (PSA) is a promising polymer for the production of drug delivery vehicles. The aim of this work is to study the effect of preparation parameters on the quality of the nanoparticles. In this study, doxorubicin (DOX)-loaded PSA nanocapsules were prepared by an emulsion method. Effects of factors such as type of organic solvent, co-solute (surfactant) and its concentration on drug-loading efficiency, particle size and size distribution, morphology and release profile were examined to gain insight in the preparation and stability of nanostructures. Particles with sizes in the range of 218-1198 nm were prepared. The smallest particles with a narrow size distribution were prepared by using polyvinyl alcohol as a co-solute and dichloromethane as a solvent. Efficiency and intracellular release of doxorubicin from the formulated particles were studied on MDA-MB-231 cells. It was observed that DOX-loaded PSA particles can diffuse into the cells and intracellular antitumour activity is directly related to the released amount of drug from the PSA nanocapsules. [ABSTRACT FROM AUTHOR]

Published
2015
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193. STAMP2 increases oxidative stress and is critical for prostate cancer.

Author

Jin, Yang, Wang, Ling, Qu, Su, Sheng, Xia, Kristian, Alexandr, Mælandsmo, Gunhild M, Pällmann, Nora, Yuca, Erkan, Tekedereli, Ibrahim, Gorgulu, Kivanc, Alpay, Neslihan, Sood, Anil, Lopez‐Berestein, Gabriel, Fazli, Ladan, Rennie, Paul, Risberg, Bjørn, Wæhre, Håkon, Danielsen, Håvard E, Ozpolat, Bulent, and Saatcioglu, Fahri

Abstract

The six transmembrane protein of prostate 2 ( STAMP2) is an androgen-regulated gene whose mRNA expression is increased in prostate cancer ( PCa). Here, we show that STAMP2 protein expression is increased in human PCa compared with benign prostate that is also correlated with tumor grade and treatment response. We also show that STAMP2 significantly increased reactive oxygen species ( ROS) in PCa cells through its iron reductase activity which also depleted NADPH levels. Knockdown of STAMP2 expression in PCa cells inhibited proliferation, colony formation, and anchorage-independent growth, and significantly increased apoptosis. Furthermore, STAMP2 effects were, at least in part, mediated by activating transcription factor 4 ( ATF4), whose expression is regulated by ROS. Consistent with in vitro findings, silencing STAMP2 significantly inhibited PCa xenograft growth in mice. Finally, therapeutic silencing of STAMP2 by systemically administered nanoliposomal si RNA profoundly inhibited tumor growth in two established preclinical PCa models in mice. These data suggest that STAMP2 is required for PCa progression and thus may serve as a novel therapeutic target. [ABSTRACT FROM AUTHOR]

Published
2015
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194. In Vivo 31P magnetic resonance spectroscopic imaging (MRSI) for metabolic profiling of human breast cancer xenografts.

Author

Esmaeili, Morteza, Moestue, Siver A., Hamans, Bob C., Veltien, Andor, Kristian, Alexandr, Engebråten, Olav, Mælandsmo, Gunhild M., Gribbestad, Ingrid S., Bathen, Tone F., and Heerschap, Arend

Abstract

Purpose To study cancer associated with abnormal metabolism of phospholipids, of which several have been proposed as biomarkers for malignancy or to monitor response to anticancer therapy. We explored 3D 31P magnetic resonance spectroscopic imaging (MRSI) at high magnetic field for in vivo assessment of individual phospholipids in two patient-derived breast cancer xenografts representing good and poor prognosis (luminal- and basal-like tumors). Materials and Methods Metabolic profiles from luminal-like and basal-like xenograft tumors were obtained in vivo using 3D 31P MRSI at 11.7T and from tissue extracts in vitro at 14.1T. Gene expression analysis was performed in order to support metabolic differences between the two xenografts. Results In vivo 31P MR spectra were obtained in which the prominent resonances from phospholipid metabolites were detected at a high signal-to-noise ratio (SNR >7.5). Metabolic profiles obtained in vivo were in agreement with those obtained in vitro and could be used to discriminate between the two xenograft models, based on the levels of phosphocholine, phosphoethanolamine, glycerophosphocholine, and glycerophosphoethanolamine. The differences in phospholipid metabolite concentration could partly be explained by gene expression profiles. Conclusion Noninvasive metabolic profiling by 3D 31P MRSI can discriminate between subtypes of breast cancer based on different concentrations of choline- and ethanolamine-containing phospholipids. J. Magn. Reson. Imaging 2015;41:601-609. © 2014 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Published
2015
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196. P50: Profiling DNA copy number changes in sarcomas by array comparative genomic hybridisation

Author

Meza-Zepeda, Leonardo A., primary, Kresse, Stine H., additional, Barragan-Polania, Ana H., additional, Tveito, Siri, additional, Wang, Junbai, additional, Kristiansen, Bjørn E., additional, Kuo, Wen-Lin, additional, Maelandsmo, Gunhild M., additional, Fodstad, Oystein, additional, Gregory, Simon G., additional, Gray, Joe W., additional, and Myklebost, Ola, additional

Published
2005
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