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We have conducted the study of ultrastructural changes of wall and cytoplasm of Plantago lanceolata (English plantain) pollen grains during the first 10 min of hydration and activation proc...

Light cycle oil (LCO) is an inexpensive feedstock for the production of high-added-commercial-value-mono-aromatic compounds such as benzene, toluene and xylenes (BTX). To extend the knowledge on the processing of LCO for BTX production, the hydrocracking reaction was studied using a commercial NiMo/Al2O3 catalyst, ZSM-5 zeolite and their mechanical mixtures (20/80, 30/70 and 50/50) for processing tetralin as model feedstock in a bench-scale-trickle-bed reactor at 450–500 °C, 3.9–5.9 MPa, 1.3 1/h and H2/feed volume ratio of 168–267 m3/m3. Accessible, well-dispersed and strong Brönsted acid sites eased the hydrocracking of tetralin to BTX and the metallic hydrogenation functions from nickel–molybdenum catalysts were also required to minimize deactivation. To achieve suitable tetralin conversions (86–95 wt%), high BTX selectivity in the liquid phase (44–70 wt%) and suitable catalytic activities for coke precursor hydrogenation (to reduce deactivation), NiMo/Al2O3//ZSM-5 mixtures (50–80 ZSM-5) were employed, which probed to be effective.

Furfural is a toxic compound that can be found in a wide variety of everyday food and beverages, notably espresso coffee. Given the toxicity of furfural, exposure could lead to liver and skin cancer. Recently, graphene oxide (GO) has been used for application in Solid Phase Micro-Extraction (SPME) due to its large surface area and wide variety of oxygenated functional groups, which makes it an excellent support for the adsorption of different organic compounds. Therefore, in this work a thermally reduced graphene oxide coating (rGOC) was synthetized for the micro-extraction of furfural in espresso coffee. This rGOC was able to absorb the furfural from espresso coffee despite having thickness of around only 1.9 μm. An analysis and optimization of extraction conditions of the furfural in the espresso coffee was carried out and then applied to the quantification of furfural. The furfural was quantified using a Gas Chromatograph coupled to a Mass Spectrometer (GC–MS). The concentration of furfural found was 2.1724 ± 0.0837 mg/L using rGOC whereas with commercial DVB/CAR/PDMS fiber was 2.0766 ± 0.0444 mg/L. The present work attempts to expand the applications of GO in SPME through the analysis of furfural in complex matrix foodstuffs such as espresso coffee. The application of rGOC, as presented in this article, has the advantage of being easy to prepare, requires a low quantity of material and together with its low cost, such application can easily be used instead of commercial fibers.

The present work shows the properties of BaTiO3:Ho3+ and BaTiO3:Ho3+/Yb3+ phosphors, prepared by the sol-gel method. The samples were characterized by powder X-ray diffraction (PXRD), Raman spectroscopy and scanning electronic microscopy (SEM). Upconversion (UC) and second harmonic generation (SHG) were monitored by photoluminescence. The UC mechanism was studied by time-resolved spectroscopy. Through PXRD, it was possible to determine that all synthesized phases crystallized in a cubic perovskite-type structure. Raman spectra corroborated the coexistence of both cubic and tetragonal phases. For the BaTiO3:Ho3+ samples excited at 1200 and 1150 nm, the emission spectra exhibited only one peak corresponding to the second harmonic of the emission wavelength. On the other hand, for the BaTiO3:Ho3+/Yb3+ samples excited at 975 nm, the emission spectra exhibited an intense green band at 554 nm, a medium intensity red band at 665 nm and a weak band at 760 nm. The study of the dependence of UC emission intensity on the pump power of the incident laser and the decay curves of red and green emissions were used to elucidate the UC mechanism, which corresponded to the process known as ground state absorption/energy transfer upconversion.

The corrosion behaviour of microporous nickel-chromium systems was studied by means of conventional electrochemical techniques and localized ones using two chloride based electrolytes with and without cupric ions in their composition. The conscious combination of different methodologies using these techniques has provided valuable information about the corrosion process. Open Circuit Potential (OCP) and Electrochemical Impedance Spectroscopy (EIS) measurements were performed in bulk solution, whilst Scanning Kelvin Probe (SKP) measurement were carried out using two methodologies: i) electrolyte droplets monitoring (measuring simultaneously potential and droplet height with time), and ii) potential maps of dried surfaces that previously were exposed to droplets. Further characterization was done based on the morphology of the attack and composition on the surface by Optical Microscopy (OM), Field Emission- Scanning Electron Microscope (FE-SEM) and X-ray Photoelectron Spectroscopy (XPS) together with the analysis of electrolyte composition with time by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Results have shown the harmful effect of Cu2+ cations in the corrosion resistance and a different morphological impact on the surface. Such negative effect has revealed a new time constant at high frequency in the impedance diagrams as well as an abrupt potential decrease (due to a change in the cathodic reaction involved: Cu2+ + e− ↔ Cu+ takes place) using SKP droplet test. Apparently, Cu+ species were stabilized in bulk solution by the formation of chloride complexes, as was confirmed by the precipitation of a white CuCl compound during droplet evaporation, pointing out the key role of Cu+ in the corrosion process.

There has been growing research interest in what we term empathy-based stress, a process of traumatic stressor exposure, empathic experience, and adverse reactions among particular empathy-related professions, captured in the literatures on compassion fatigue, secondary traumatic stress, and vicarious traumatization in trauma-related lines of work. Although these three empathy-based strain constructs are highly related, each represents different components of similar strain responses. Unfortunately, extant reviews of the empathy-based stress literature are non-comprehensive and/or out of date. This qualitative review thus aims to synthesize and summarize the current literature on empathy-based stress at work and contribute to theoretical, methodological, and practical improvements in this area of research and practice. After introducing empathy-based strain constructs and their defining characteristics, we detail our review methodology and the primary theoretical and empirical themes derived through our review of the past decade of published literature. Then, we summarize conceptual, methodological, and analytical gaps in the empathy-based stress literature, helping to generate recommendations for the literature moving forward.

Three-dimensional graphene materials (3D) have attracted great interest in recent years due to the outstanding properties that are product of the combination between graphene intrinsic properties and 3D materials macroscopic properties, such as porosity and easy manipulation due to their macroscopic scale dimensions. These characteristics have given them notorious advantage for various applications over other materials. This study reports a methodology to obtain a 3D material made with reduced graphene oxide (rGO) using spherical particles of calcium carbonate (CaCO3) as sacrificial template. CaCO3 particles were coated with graphene oxide (GO), using a layer-by-layer procedure (LbL) and later, templates were removed using acid solution. The obtained monolith retains the shape of the templates. The morphology, thermal stability and chemical composition of the monolith were characterized by several techniques: X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), X-ray photoemission spectroscopy (XPS), Raman spectroscopy and infrared spectroscopy (FTIR). The results showed that the obtained monolith has spherical hollow spaces distributed throughout the 3D structure. In addition, physisorption analysis using N2 revealed that the obtained monolith increased 40% its surface area compared to a conventional rGO aerogel prepared without using sacrificial templates.

Organic semiconductors are of interest for (opto)electronic applications due to their low cost, solution processability, and tunable properties. Recently, natural product-derived organic pigments attracted attention due to their extraordinary environmental stability and unexpectedly good optoelectronic performance, in spite of only partially conjugated molecular structure. Fungi-derived pigments are a naturally sourced, sustainable class of materials that are largely unexplored as organic semiconductor materials. We present a study of the optical and electronic properties of a fungi-derived pigment xylindein, which is secreted by the wood-staining fungi Chlorociboria aeruginosa, and its blends with poly(methyl methacrylate) (PMMA) and crystalline nanocellulose (CNC). Optical absorption spectra of xylindein revealed the presence of two tautomers whose structures and properties were established using density functional theory. Pronounced pigment aggregation in polar solvents and in films, driven by intermolecular hydrogen bonding, was also observed. The pigment exhibited high photostability, electron mobility up to 0.4 cm2/(V s) in amorphous films, and thermally activated charge transport and photoresponse with activation energies of ∼0.3 and 0.2 eV, respectively. The dark and photocurrents in xylindein:PMMA blends were comparable to those in pristine xylindein film, whereas blends with CNC exhibited lower currents due to inhomogeneous distribution of xylindein in the CNC.

A novel waterborne coating has been successfully designed by combining good barrier properties and a built-in ability to in situ phosphatize low carbon steel substrates. The physical barrier protec...

Two moderately halophilic and psychrotolerant new Mycoplasma species were isolated from common cephalopods. Three strains were isolated in pure culture from two individual European flying squid (Todarodes sagittatus), and two individual octopuses (Octopus vulgaris). The strains showed optimal growth at 25 °C and a salinity of 3% (w/v) NaCl. Molecular analyses revealed that the isolates belonged to two new, but phylogenetically related species, divergent from all previously described Mollicutes, representing the first marine isolates of the class, and also the first Mycoplasma strains for which NaCl requirement has been demonstrated. A genome search against all available marine metagenomes and 16S rRNA gene databases indicated that these two species represent a novel non-free-living marine lineage of Mollicutes, specifically associated with marine animals. Morphology and physiology were compatible with other members of this group, and genomic and phenotypic analyses demonstrated that these organisms represent two novel species of the genus Mycoplasma, for which the names Mycoplasma marinum sp. nov. and Mycoplasma todarodis sp. nov. are proposed; the type strains are PET (DSM 105487T, CIP 111404T) and 5HT (DSM 105,488T, CIP 111405T), respectively., RRM acknowledges the Spanish Ministry of Economy project CLG2015_66686-C3-1-P also supported with European Regional Development Fund (FEDER) funds. ASR acknowledges the Gobierno de Canarias (Spain) project P2007/046. RRM acknowledges the financial support of the sabbatical stay at Georgia Institute of Technology supported by the grant PRX18/00048 of the Ministry of Sciences, Innovation and Universities. KTK’s research was supported, in part, by the U.S. National Science Foundation (Award No. 1,759,831).

Introduction:Demyelinating diseases of the central nervous system (CNS) comprise a group of neurological disorders characterized by progressive (and eventually irreversible) loss of oligodendrocytes and myelin sheaths in the white matter tracts. Some of myelin disorders include: Multiple sclerosis, Guillain-Barré syndrome, peripheral nerve polyneuropathy and others. To date, the etiology of these disorders is not well known and no effective treatments are currently available against them. Therefore, further research is needed to gain a better understand and treat these patients. To accomplish this goal, it is necessary to have appropriate animal models that closely resemble the pathophysiology and clinical signs of these diseases. Herein, we describe the model of toxic demyelination induced by cuprizone (CPZ), a copper chelator that reduces the cytochrome and monoamine oxidase activity into the brain, produces mitochondrial stress and triggers the local immune response. These biochemical and cellular responses ultimately result in selective loss of oligodendrocytes and microglia accumulation, which conveys to extensive areas of demyelination and gliosis in corpus callosum, superior cerebellar peduncles and cerebral cortex. Remarkably, some aspects of the histological pattern induced by CPZ are similar to those found in multiple sclerosis. CPZ exposure provokes behavioral changes, impairs motor skills and affects mood as that observed in several demyelinating diseases. Upon CPZ removal, the pathological and histological changes gradually revert. Therefore, some authors have postulated that the CPZ model allows to partially mimic the disease relapses observed in some demyelinating diseases.Conclusion:for five decades, the model of CPZ-induced demyelination is a good experimental approach to study demyelinating diseases that has maintained its validity, and is a suitable pharmacological model for reproducing some key features of demyelinating diseases, including multiple sclerosis.

The synthesis and surface-specific spectroscopic analysis of α-pinene isotopologues and their corresponding secondary organic material is reported., Atmospheric aerosol–cloud interactions remain among the least understood processes within the climate system, leaving large uncertainties in the prediction of future climates. In particular, the nature of the surfaces of aerosol particles formed from biogenic terpenes, such as α-pinene, is poorly understood despite the importance of surface phenomena in their formation, growth, radiative properties, and ultimate fate. Herein we report the coupling of a site-specific deuterium labeling strategy with vibrational sum frequency generation (SFG) spectroscopy to probe the surface C–H oscillators in α-pinene-derived secondary organic aerosol material (SOM) generated in an atmospheric flow tube reactor. Three α-pinene isotopologues with methylene bridge, bridgehead methine, allylic, and vinyl deuteration were synthesized and their vapor phase SFG spectra were compared to that of unlabeled α-pinene. Subsequent analysis of the SFG spectra of their corresponding SOM revealed that deuteration of the bridge methylene C–H oscillators present on the cyclobutane ring in α-pinene leads to a considerable signal intensity decrease (ca. 30–40%), meriting speculation that the cyclobutane moiety remains largely intact within the surface bound species present in the SOM formed upon α-pinene oxidation. These insights provide further clues as to the complexity of aerosol particle surfaces, and establish a framework for future investigations of the heterogeneous interactions between precursor terpenes and particle surfaces that lead to aerosol particle growth under dynamically changing conditions in the atmosphere.

Biogenic aerosols may play an active role in various diseases. Pollutant gases and bioaerosols coexist in the atmosphere with the possibility of interacting with each other increasing their adverse impacts on human health. The study of long-term trends and the correlation between the pollen concentration from selected taxa (especially those related to allergies) and both the main atmospheric pollutants and the meteorological parameters has enabled us to identify the main factors that affect pollen concentration in the atmosphere. This study analyzes the long-term trend in CO, NO, NO2, PM10, SO2 and O3 from 1997 to 2016 and Fraxinus, Poaceae and Populus pollen concentrations in the city of Leon from 1994 to 2016. In general, there is a significant decreasing trend in atmospheric pollutant concentrations and a significant increasing trend in Fraxinus pollen concentrations. In addition, the influence of air pollutants and climatic factors on pollen concentrations and pollination period duration was studied using the Spearman correlation, showing that the flowering and pollination periods depend largely of the weather conditions before these periods and are influenced by air pollutant concentrations.

Traumatic brain injury (TBI) is associated with increased risk for mental health disorders, impacting post-injury quality of life and societal reintegration. TBI is also associated with deficits in psychosocial processing, defined as the cognitive integration of social and emotional behaviors, however little is known about how these deficits manifest and their contributions to post-TBI mental health. In this pre-clinical investigation using rats, a single mild blast TBI (mbTBI) induced impairment of psychosocial processing in the absence of confounding physical polytrauma, post-injury motor deficits, affective abnormalities, or deficits in non-social behavior. Impairment severity correlated with acute upregulations of a known oxidative stress metabolite, 3-hydroxypropylmercapturic acid (3-HPMA), in urine. Resting state fMRI alterations in the acute post-injury period implicated key brain regions known to regulate psychosocial behavior, including orbitofrontal cortex (OFC), which is congruent with our previous report of elevated acrolein, a marker of neurotrauma and 3-HPMA precursor, in this region following mbTBI. OFC of mbTBI-exposed rats demonstrated elevated mRNA expression of metabotropic glutamate receptors 1 and 5 (mGluR1/5) and injection of mGluR1/5-selective agonist in OFC of uninjured rats approximated mbTBI-induced psychosocial processing impairment, demonstrating a novel role for OFC in this psychosocial behavior. Furthermore, OFC may serve as a hotspot for TBI-induced disruption of psychosocial processing and subsequent mental health disorders.

A new sampling technique for the application of proper orthogonal decomposition to a set of snapshots has been recently developed by the authors to facilitate a variety of data processing tasks (J. Comput. Phys. 335, 2017). According to it, robust modal expansions result from performing the decomposition on a limited number of relevant snapshots and a limited number of discretization mesh points, which are selected via Gauss elimination with double pivoting on the original snapshot matrix containing the given data. In the present work, the sampling method is adapted and combined with low-dimensional modeling. This combination yields a novel adaptive algorithm for the simulation of time-dependent non-linear dynamics in pattern-forming systems. Convenient snapshot sets, computed on demand over the evolution, are stored to record local temporal events whose underlying mechanisms are essential for the approximations. Also, a collection of sparse grid points, which are used to construct the mode basis and the reduced system of equations, is adaptively sampled according to unlinked spatial structures. The outcome is a reduced order model of the problem that (i) yields reliable approximations of the dynamical transitions, (ii) is well-suited to describe localized spatio-temporal complexity, and (iii) provides fast computations. Robustness, accuracy, and computational efficiency of the proposed algorithm are illustrated for some relevant pattern-forming systems, in both one and two spatial dimensions, exhibiting solutions with a rich spatio-temporal structure.

UV-light degradation of wood is one of the top reasons for consumer replacement of outdoor wooden structures. This type of degradation is seldom mechanical, and is instead often motivated by loss of aesthetics (graying). There are numerous commercial products available on the market that deal with this loss of color, many of which contain added pigments to ‘rejuvenate’ or ‘revitalize’ greyed wood. These pigments are almost uniformly synthetic. In contrast, pigments from wood decay fungi (spalting), which have been used in woodworking since the 1400s (intarsia), have remarkable optical (UV-light resistance) properties due to their naphthoquinonic configuration. In recent years the pigments made from these fungi have been extracted and tested across numerous substrates, from solar cells to textile dyes. In this work, researchers extracted pigments from Scytalidium cuboideum (red pigmentation) and Chlorociboria aeruginosa (blue-green pigmentation), solubilized the pigments in raw linseed oil, and tested the resulting solution on samples of Douglas-fir (Pseudotsuga menziesii) and western white pine (Pinus monticola). These mixtures were compared against a ‘stain and coat’ treatment (utilizing an aniline stain and coated with raw linseed oil), raw linseed oil, and untreated wood. The wood samples were then placed in an accelerated weathering machine (Q-UV) following the ASTM G154 standard, for 500 and 1000 h. The results showed that while no visible color change occurred to the wood when the pigmented oil was applied, the red pigment oil significantly lowered the coating degradation for both wood types at an exposure of 500 h. The results show the potential applications for fungal pigments in the wood coating industry, as it offers an increased coating service life. As there is a shift to renewable products, the pigments from wood decay fungi show potential as additives for wood coatings.

Synopsis Animals live in heterogeneous environments must navigate in order to forage or capture food, defend territories, and locate mates. These heterogeneous environments have a variety of substrates that differ in their roughness, texture, and other properties, all of which may alter locomotor performance. Despite such natural variation in substrate, many studies on locomotion use noncompliant surfaces that either are unrepresentative of the range of substrates experienced by species or underestimate maximal locomotor capabilities. The goal of this study was to determine the role of forefeet and hindfeet on substrates with different properties during walking in a generalized sprawling tetrapod, the tiger salamander (Ambystoma tigrinum). Adult salamanders (n = 4, SVL = 11.2–14.6 cm) walked across level dry sand (DS), semi-soft plaster of Paris (PoP), wet sand (WS), and a hard, noncompliant surface (table)—substrates that vary in compliance. Trials were filmed in dorsal and anterior views. Videos were analyzed to determine the number of digits and surface area of each foot in contact with the substrate. The surface area of the forelimbs contacting the substrate was significantly greater on DS and PoP than on WS and the table. The surface area of the hindlimbs contacting the substrate was significantly greater on DS than on all other substrates. There were no significant differences in the time that the fore- or hindfeet were in contact with the substrate as determined by the number of digits. We conclude that salamanders modulate the use of their feet depending on the substrate, particularly on DS which is known to increase the mechanical work and energy expended during locomotion owing to the fluid nature of its loose particles. More studies are needed to test a wider range of substrates and to incorporate behavioral data from field studies to get a better understanding of how salamanders are affected by different substrates in their natural environment.

CeO2 nanoparticles were incorporated in waterborne binders containing high biobased content (up to 70%) in order to analyze the anticorrosion performance for direct to metal coatings. Biobased binders were synthesized by batch miniemulsion polymerization of 2-octyl acrylate and isobornyl methacrylate monomers using a phosphate polymerizable surfactant (Sipomer PAM200) that lead to the formation of phosphate functionalized latexes. Upon the direct application of such binders on steel, the functionalized polymer particles were able to interact with steel, creating a thin phosphatization layer between the metal and the polymer and avoiding flash rust. The in situ incorporation of the CeO2 nanoparticles during the polymerization process led to their homogeneous distribution in the final polymer film, which produced outstanding anticorrosion performance according to the Electrochemical Impedance Spectroscopy measurements. In fact, steel substrates coated with the hybrid polymer film (30–40 µm thick) showed high barrier corrosion resistance after 41 days (~1000 h) of immersion in NaCl water solution and active inhibition capabilities thanks to the presence of the CeO2 nanoparticles. This work opens the door to the fabrication of sustainable hybrid anticorrosion waterborne coatings. This research was funded by the Spanish Government, grant numbers MINECO CTQ-2017-87841-R and CER-20191003, and by the Basque Government “Grupos Consolidados del Sistema Universitario Vasco”, grant number IT999-16.

Graphene oxide (GO) is a carbon material with wide chemistry, and its functionalization represents the main challenge to develop multi-functional materials for different applications. Besides to epoxide and carboxyl groups, the alkenes that arise during the graphite oxidation seem to provide an excellent approach for developing selective reactions. In specific, α,β-unsaturated acids can be functionalized by thiol-ene Michael addition (TEMA) using base catalysts. However, when base catalysts are used, the thiol-epoxide ring opening reaction (TEROR) has been considered as the predominant and unique reaction. Therefore, there is a need to understand the reactivity of the GO when TEMA and TEROR take place from a qualitative and quantitative point of view. Herein, we studied the functionalization of GO and reduced graphene oxide (RGO) with cysteamine (CA) using different base catalysts. XPS results revealed that despite the thermal reduction of GO, the functionalization was possible confirming the coupling reaction between the CA and α,β-unsaturated acids of RGO by TEMA. In addition, a quantitative analysis was conducted via fluorescent labelling corroborating that TEMA produced more CA molecules bonded to GO than TEROR.

Objectives: Estimates of depression prevalence in pregnancy and postpartum are based on the Edinburgh Postnatal Depression Scale (EPDS) more than on any other method. We aimed to determine if any EPDS cutoff can accurately and consistently estimate depression prevalence in individual studies. Methods: We analyzed datasets that compared EPDS scores to Structured Clinical Interview for DSM (SCID) major depression status. Random‐effects meta‐analysis was used to compare prevalence with EPDS cutoffs versus the SCID. Results: Seven thousand three hundred and fifteen participants (1017 SCID major depression) from 29 primary studies were included. For EPDS cutoffs used to estimate prevalence in recent studies (≥9 to ≥14), pooled prevalence estimates ranged from 27.8% (95% CI: 22.0%–34.5%) for EPDS ≥ 9 to 9.0% (95% CI: 6.8%–11.9%) for EPDS ≥ 14; pooled SCID major depression prevalence was 9.0% (95% CI: 6.5%–12.3%). EPDS ≥14 provided pooled prevalence closest to SCID‐based prevalence but differed from SCID prevalence in individual studies by a mean absolute difference of 5.1% (95% prediction interval: 13.7%, 12.3%). Conclusion: EPDS ≥14 approximated SCID‐based prevalence overall, but considerable heterogeneity in individual studies is a barrier to using it for prevalence estimation., This study was funded by the Canadian Institutes of Health Research (CIHR, KRS‐140994). Ms. Lyubenova was supported by the Mitacs Globalink Research Internship Program. Ms. Neupane was supported by G.R. Caverhill Fellowship from the Faculty of Medicine, McGill University. Drs. Levis and Wu were supported by Fonds de recherche du Québec‐Santé (FRQS) Postdoctoral Training Fellowships. Mr. Bhandari was supported by a studentship from the Research Institute of the McGill University Health Centre. Ms. Rice was supported by a Vanier Canada Graduate Scholarship. Ms. Azar was supported by a FRQS Masters Training Award. The primary study by Barnes et al. was supported by a grant from the Health Foundation (1665/608). The primary study by Beck et al. was supported by the Patrick and Catherine Weldon Donaghue Medical Research Foundation and the University of Connecticut Research Foundation. The primary study by Helle et al. was supported by the Werner Otto Foundation, the Kroschke Foundation, and the Feindt Foundation. Prof. Robertas Bunevicius, MD, PhD (1958‐2016) was Principal Investigator of the primary study by Bunevicius et al., but passed away and was unable to participate in this project. The primary study by Chaudron et al. was supported by a grant from the National Institute of Mental Health (grant K23 MH64476). The primary study by Tissot et al. was supported by the Swiss National Science Foundation (grant 32003B 125493). The primary study by Tendais et al. was supported under the project POCI/SAU‐ESP/56397/2004 by the Operational Program Science and Innovation 2010 (POCI 2010) of the Community Support Board III and by the European Community Fund FEDER. The primary study by Garcia‐Esteve et al. was supported by grant 7/98 from the Ministerio de Trabajo y Asuntos Sociales, Women's Institute, Spain. The primary study by Howard et al. was supported by the National Institute for Health Research (NIHR) under its Programme Grants for Applied Research Programme (Grant Reference Numbers RP‐PG‐1210‐12002 and RP‐DG‐1108‐10012) and by the South London Clinical Research Network. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR or the Department of Health and Social Care. The primary study by Phillips et al. was supported by a scholarship from the National Health and Medical and Research Council (NHMRC). The primary study by Nakić Radoš et al. was supported by the Croatian Ministry of Science, Education, and Sports (134‐0000000‐2421). The primary study by Navarro et al. was supported by grant 13/00 from the Ministry of Work and Social Affairs, Institute of Women, Spain. The primary study by Pawlby et al. was supported by a Medical Research Council UK Project Grant (number G89292999N). The primary study by Quispel et al. was supported by Stichting Achmea Gezondheid (grant number z‐282). Dr. Robertson‐Blackmore was supported by a Young Investigator Award from the Brain and Behavior Research Foundation and NIMH grant K23MH080290. The primary study by Rochat et al. was supported by grants from the University of Oxford (HQ5035), the Tuixen Foundation (9940), the Wellcome Trust (082384/Z/07/Z and 071571), and the American Psychological Association. Dr. Rochat receives salary support from a Wellcome Trust Intermediate Fellowship (211374/Z/18/Z). The primary study by Prenoveau et al. was supported by The Wellcome Trust (grant number 071571). The primary study by Stewart et al. was supported by Professor Francis Creed's Journal of Psychosomatic Research Editorship fund (BA00457) administered through University of Manchester. The primary study by Tandon et al. was funded by the Thomas Wilson Sanitarium. The primary study by Tran et al. was supported by the Myer Foundation who funded the study under its Beyond Australia scheme. Dr. Tran was supported by an early career fellowship from the Australian National Health and Medical Research Council. The primary study by Vega‐Dienstmaier et al. was supported by Tejada Family Foundation, Inc, and Peruvian‐American Endowment, Inc. Drs. Benedetti and Thombs were supported by FRQS researcher salary awards.

A review of available results on non-destructive testing of physical systems, using the concept of topological sensitivity, is presented. This mathematical tool estimates the sensitivity of a set of measurements in some given sensors, distributed along the system, to defects/flaws that produce a degradation of the system. Such degradation manifests itself on the properties of the system. The good performance of this general purpose post-processing method is reviewed and illustrated in some applications involving non-destructive testing. These applications include structural health monitoring, considering both elastodynamic ultrasonic guided Lamb waves and active infrared thermography. Related methods can also be used in other fields, such as diagnosis/prognosis of engineering devices, which is also considered.

Haloarchaea produce C50 carotenoids such as bacterioruberin, which are of biotechnological in-terest. This study aimed to analyze the effect of different environmental and nutritional conditions on the cellular growth and dynamics of carotenoids accumulation in Haloferax mediterranei. The maximum production of carotenoids (40 µg·mL−1) was obtained during the stationary phase of growth, probably due to nutrient-limiting conditions (one-step culture). By seven days of culture, 1 mL culture produced 22.4 mg of dry weight biomass containing 0.18 % (w/w) of carotenoids. On the other hand, carbon-deficient cultures (low C/N ratio) were observed to be optimum for C50 bacterioruberin production by Hfx. mediterranei, but negatively affected the growth of cells. Thus, a two-steps process was evaluated for optimum carotenoids yield. In the first step, a nutri-ent-repleted culture medium enabled the haloarchaea to produce biomass, while in the second step, the biomass was incubated under osmotic stress and in a carbon-deficient medium. Under the conditions used, the obtained biomass contained 0.27% (w/w) of carotenoids after seven days, which accounts for 58.49 µg·mL−1 of carotenoids for a culture with turbidity 14.0. This work was funded by a research grant from MINECO Spain (RTI2018-099860-B-I00) and the University of Alicante (VIGROB-309). We are also indebted to the Andalusian Government (research project BIO-214). ZM was assisted by a pre-doctoral grant from “Plan Propio de Investigación” of the University of Huelva, Spain. MG was awarded with a pre-doctoral fellowship from the Valencian Community Government (ACIF/2019/043).

Nowadays there is not an effective drug for the treatment of infections caused by human adenovirus (HAdV) which supposes a clinical challenge, especially for paediatric and immunosuppressed patients. Here, we describe the design, synthesis and biological evaluation as anti-adenovirus agents of a new library (57 compounds) of diester, monoester and triazole derivatives based on 3-amino-1,2-propanediol skeleton. Seven compounds (17, 20, 26, 34, 44, 60 and 66) were selected based on their high anti-HAdV activity at low micromolar concentration (IC50 from 2.47 to 5.75 µM) and low cytotoxicity (CC50 from 28.70 to >200 µM). In addition, our mechanistic assays revealed that compounds 20 and 44 might be targeting specifically the HAdV DNA replication process, and compound 66 would be targeting HAdV E1A mRNA transcription. For compounds 17, 20, 34 and 60, the mechanism of action seems to be associated with later steps after HAdV DNA replication., This work has been supported by Ministerio de Ciencia, Innovación y Universidades, Plan Estatal 2017-2020 Retos - Proyectos I + D + i (PID2019-104767RB-I00); Ministerio de Economía y Competitividad, Plan Nacional I + D + i 2013-2016, Retos-Proyectos (CTQ2016-78580-C2-2-R); Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/0009), cofinanced by European Development Regional Fund “A way to achieve Europe”, the Instituto de Salud Carlos III, Proyectos de Investigación en Salud (PI17/01055; PI18/01191) and Proyectos de Desarrollo Tecnológico en Salud (DTS17/00130), the Spanish Adenovirus Network (AdenoNet, BIO2015/68990-REDT), and the program “Nicolás Monardes” (C-0059-2018), Servicio Andaluz de Salud, Junta de Andalucía.

Synopsis Lateral undulation and trunk flexibility offer performance benefits to maneuverability, stability, and stride length (via speed and distance traveled). These benefits make them key characteristics of the locomotion of tetrapods with sprawling posture, with the exception of turtles. Despite their bony carapace preventing lateral undulations, turtles are able to improve their locomotor performance by increasing stride length via greater limb protraction. The goal of this study was to quantify the effect of reduced lateral flexibility in a generalized sprawling tetrapod, the tiger salamander (Ambystoma tigrinum). We had two potential predictions: (1) either salamanders completely compensate by changing their limb kinematics, or (2) their performance (i.e., speed) will suffer due to the reduced lateral flexibility. This reduction was performed by artificially limiting trunk flexibility by attaching a 2-piece shell around the body between the pectoral and pelvic girdles. Adult tiger salamanders (n = 3; SVL = 9–14.5 cm) walked on a 1-m trackway under three different conditions: unrestricted, flexible shell (Tygon tubing), and rigid shell (PVC tubing). Trials were filmed in a single, dorsal view, and kinematics of entire midline and specific body regions (head, trunk, tail), as well as the fore and hind limbs, were calculated. Tygon individuals had significantly higher curvature than both PVC and unrestricted individuals for the body, but this trend was primarily driven by changes in tail movements. PVC individuals had significantly lower curvature in the trunk region compared with unrestricted individuals or Tygon; however, there was no difference between unrestricted and Tygon individuals suggesting the shells performed as expected. PVC and Tygon individuals had significantly higher curvature in the tails compared with unrestricted individuals. There were no significant differences for any limb kinematic variables among treatments including average, minimum, and maximum angles. Thus, salamanders respond to decreased lateral movement in their trunk by increasing movements in their tail, without changes in limb kinematics. These results suggest that tail undulations may be a more critical component to sprawling-postured tetrapod locomotion than previously recognized.

This chapter applies the HODMD and STKD methods to detect the spatio-temporal patterns driving the flow in the wake of wind turbines, considering two types of applications. In the first application, HODMD and STKD will identify the main flow instabilities that permit studying the bifurcation process that occurs in the wake of the wind turbine, considering two types of wind turbines, one with vertical axis and another with horizontal axis. Using SKTD, it is possible to simplify the description of the complex flow in the wake as a small group of well organized traveling waves. In the second application, the HODMD algorithm is slightly modified to predict, at different spatial locations, the temporal evolution of the wind flow found upstream a wind turbine with horizontal axis. This second application uses actual LiDAR experimental data. Finally, the readers will create their own model for data forecasting in wind turbines using a toy model.

The G-protein coupled receptors (GPCRs) activated by free fatty acids (FFAs) have emerged as new and exciting drug targets, due to their plausible translation from pharmacology to medicines. This perspective aims to report recent research about GPR120/FFAR4 and its involvement in several diseases, including cancer, inflammatory conditions, and central nervous system disorders. The focus is to highlight the importance of GPR120 in Type 2 diabetes mellitus (T2DM). GPR120 agonists, useful in T2DM drug discovery, have been widely explored from a structure-activity relationship point of view. Since the identification of the first reported synthetic agonist TUG-891, the research has paved the way for the development of TUG-based molecules as well as new and different chemical entities. These molecules might represent the starting point for the future discovery of GPR120 agonists as antidiabetic drugs.

This paper deals with the data-driven reduced order modeling of high dimensional systems, using a tensor decomposition plus one-dimensional interpolation. The (many) involved dimensions are usually associated with space, and/or time, and/or various parameters the system may depend on. Three tensor decomposition methods are considered, namely recursive proper orthogonal decomposition, higher order singular value decomposition, and proper generalized decomposition. The former method exhibits a well-established mathematical foundation (namely, rigorous error estimates have been obtained) in the continuous limit, while rigorous error estimates for the remaining two decompositions are available in the discrete case only. The data-driven ROM is first described and its combination with each of the three tensor decompositions is evaluated using a toy model tensor. In addition, application is made to the real-time simulation of air-wall heat transfer in buildings. In this application, the performance of the data-driven ROM is compared with that of a typical empirical model, as well as with radial basis function interpolation.

Introduction: There are few published empirical data on the effects of COVID-19 on mental health, and until now, there is no large international study. ----- Material and methods: During the COVID-19 pandemic, an online questionnaire gathered data from 55,589 participants from 40 countries (64.85% females aged 35.80 ± 13.61; 34.05% males aged 34.90±13.29 and 1.10% other aged 31.64±13.15). Distress and probable depression were identified with the use of a previously developed cut-off and algorithm respectively. ----- Statistical analysis: Descriptive statistics were calculated. Chi-square tests, multiple forward stepwise linear regression analyses and Factorial Analysis of Variance (ANOVA) tested relations among variables. ----- Results: Probable depression was detected in 17.80% and distress in 16.71%. A significant percentage reported a deterioration in mental state, family dynamics and everyday lifestyle. Persons with a history of mental disorders had higher rates of current depression (31.82% vs. 13.07%). At least half of participants were accepting (at least to a moderate degree) a non-bizarre conspiracy. The highest Relative Risk (RR) to develop depression was associated with history of Bipolar disorder and self-harm/attempts (RR = 5.88). Suicidality was not increased in persons without a history of any mental disorder. Based on these results a model was developed. ----- Conclusions: The final model revealed multiple vulnerabilities and an interplay leading from simple anxiety to probable depression and suicidality through distress. This could be of practical utility since many of these factors are modifiable. Future research and interventions should specifically focus on them.

Even though magnetic resonance and aircraft flight tests are associated with two different phenomena from the physical point of view, they are mathematically similar. This is because they both intend to calculate the natural frequencies and associated damping rates of a system from its experimentally measured response to impulsive excitation. In aircraft flight tests, the goal is to identifying aeroelastic natural frequencies and damping rates via the analysis of the response of the aircraft to impulsive motion of the control surfaces. Natural aeroelastic frequencies should be avoided in actual flights to prevent dynamic instabilities, such as flutter. In magnetic resonance, the natural frequencies are oscillating frequencies of an atom nucleus (associated with its spin), and the impulsive forcing is produced by a strong magnetic field. Calculating the frequencies and damping rates permits identifying the atom, which is useful in both analytical chemistry (nuclear magnetic resonance) and medical imaging (medical magnetic resonance).

The Agave tequilana Weber is an important commercial crop in the State of Jalisco, Mexico. However, the agave cultivation generates significant soil loss. For that reason, knowledge about the implementation of the agriculture management practices, such as manure application and the combination of inorganic fertilizers and manure, are relevant. The objective of this research was to determine the effect of agricultural management practices on the total organic carbon (TOC) in the soil in three study locations: Arandas, Tepatitl&aacute, n, and Acatic in the Altos Sur region of Jalisco. A random sampling was carried out in each study location, 12 samples were obtained for each location at 0&ndash, 30 cm deep, and a total of 36 samples were analyzed. The evaluated parameters were the potential hydrogen (pH), electrical conductivity (EC), bulk density (BD), soil-water saturation (SWS), total nitrogen (TN), and total organic carbon (TOC). Basic statistics and correlations between parameters were generated. In addition, to estimate TOC from a multivariate analysis, models were developed based on the lowest Akaike information criterion (AIC) and of the classification and regression trees (CART). ANOVA and Tukey test were determined. Results demonstrated a significant difference in the TOC percentages between the study locations. The Tukey test showed that there is no difference in TOC content between the Tepatitl&aacute, n and Arandas sites, but there is a difference between these two sites and the Acatic. The latter resulted with the lowest values of TOC. Long-term studies are recommended to develop crop management strategies.

Most of the research related to Peruvian Amazon fungi is focused on edible mushrooms and pathogens. Other important fungi, such as the spalting type (decay fungi that pigment wood internally), are not broadly studied, as most of them do not produce fruiting bodies and can be difficult to locate. Spalting fungi, however, are of broad economic importance due to their ability to produce pigments that enhance wood aesthetics, resulting in an increased economic value. In order to begin understanding the diversity of spalting fungi within certain regions of the Amazon, a sampling of downed trees and branches (through the opening of the xylem to identify potential pigmenting and zone line producing fungi) was done in the district of Las Piedras, Madre de Dios, Peru. Fungi suspected of causing internal pigment and zone lines were collected, cultured, isolated, and sequenced. The species found belonged to the orders Helotiales, Xylariales, Hypocreales, Russulales, Polyporales, Botryosphaeriales and two specimens of the class Leotiomycetes. The fungi collected produced pigments or zone lines in wild conditions and all of them were capable of wood decomposition. Interestingly, these are the same orders and genera as North American spalting fungi, which may indicate a correlation within species that pigment wood. The results obtained start a specific database of spalted fungi in the Amazon and, with it, help support an effort to increase the forest value of ecosystems primarily used for a few high-valued tree species.