171 results on '"Liu, Yun-Qi"'
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152. Modified polyoxometalate: a novel monocapped bi‐supporting and reduced α‐Keggin structure {PMo12O40[Cu(2,2′‐bpy)]}[Cu(2,2′‐bpy)(en)(H2O)]2.
- Author
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Lu, Yu-Kun, Li, Ya-Ping, Yang, Ling-Yu, Wang, Wen-Hong, Pan, Yuan, Yan, Wen-Fu, and Liu, Yun-Qi
- Subjects
- *
HYDROGEN bonding , *METHYLENE blue , *X-ray diffraction , *WATER , *POLYANIONS , *CRYSTALLIZATION , *COORDINATION polymers - Abstract
A novel modified polyoxometalate, {PMo12O40[Cu(2,2′‐bpy)]}[Cu(2,2′‐bpy)(en)(H2O)]2 [2,2′‐bpy is 2,2′‐bipyridyl (C10H8N2) and en is ethylenediamine (C2H8N2)], has been synthesized hydrothermally and structurally characterized by elemental analysis, TG, IR, XPS and single‐crystal X‐ray diffraction. The structural analysis reveals that the compound contains the reduced Keggin polyanion [PMo12O40]6− as the parent unit, which is monocapped by [Cu(2,2′‐bpy)]2+ fragments via four bridging O atoms on an {Mo4O4} pit and bi‐supported by two [Cu(2,2′‐bpy)(en)(H2O)]2+ coordination cations simultaneously. There exist strong intramolecular π–π stacking between the capping and supporting units, which play a stabilizing role during the crystallization of the compound. Adjacent POM clusters are further aggregated to form a three‐dimensional supramolecular network through noncovalent forces, hydrogen bonding and π–π stacking interactions. In addition, the photocatalytic properties were investigated in detail, and the results indicated that the compound can be used as a photocatalyst towards the decomposition of the organic pollutant methylene blue (MB). [ABSTRACT FROM AUTHOR]
- Published
- 2019
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153. A facile hydrothermal synthesis and growth mechanism of novel hollow β-MnO2 polyhedral nanorods
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Ji, Zhong-Hai, Dong, Bin, Guo, Hai-Ling, Chai, Yong-Ming, Li, Yan-Peng, Liu, Yun-Qi, and Liu, Chen-Guang
- Subjects
- *
CRYSTAL growth , *MANGANESE oxides , *NANOROD synthesis , *MOLECULAR structure , *CHEMICAL sample preparation , *X-ray diffraction , *OSTWALD ripening - Abstract
Abstract: Novel hollow β-MnO2 polyhedral nanorods have been successfully synthesized by a simple hydrothermal process. The morphology and structure of the samples obtained at different reaction time have been characterized by XRD, TEM and SEM. The results indicate that β-MnO2 nanorods prepared at reaction time 12 h have hollow, polyhedral structure and uniform size with the length of about 2–3 μm. At longer reaction time, the shells of the hollow β-MnO2 polyhedral nanorods were broken. After analyzing the above results, it''s obvious that the reaction time has a great effect on the structure and morphology of the products. The morphology evolution of MnO2 products demonstrates that the growth mechanism of hollow β-MnO2 polyhedral nanorods could be assigned to the cooperation of oriented attachment and Ostwald ripening mechanism. [Copyright &y& Elsevier]
- Published
- 2012
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154. The complete chloroplast genome of Primula amethystina subsp . argutidens (Primulaceae).
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Wang SB, Liu YQ, Zhang L, Li R, and Huang Y
- Abstract
Primula amethystina subsp. argutidens (Franchet) W. W. Smith & H. R. Fletcher (1942) is a blooming plant of the family Primulaceae. Here, we sequenced, assembled, and annotated the complete chloroplast (cp) genome of P. amethystina subsp. argutidens . The cp genome of P. amethystina subsp. argutidens is 151,560 bp in length with a GC content of 37%. The assembled genome has a typical quadripartite structure, containing a large single-copy (LSC) region of 83,516 bp, a small single-copy (SSC) region of 17,692 bp, and a pair of inverted repeat (IR) regions of 25,176 bp. The cp genome contains 115 unique genes, including 81 protein-coding genes, four rRNA genes, and 30 tRNA genes. Phylogenetic analysis showed that P. amethystina subsp. argutidens was closely related to P. amethystina ., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
- Published
- 2023
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155. GDF11 promotes wound healing in diabetic mice via stimulating HIF-1ɑ-VEGF/SDF-1ɑ-mediated endothelial progenitor cell mobilization and neovascularization.
- Author
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Zhang Y, Zhang YY, Pan ZW, Li QQ, Sun LH, Li X, Gong MY, Yang XW, Wang YY, Li HD, Xuan LN, Shao YC, Li MM, Zhang MY, Yu Q, Li Z, Zhang XF, Liu DH, Zhu YM, Tan ZY, Zhang YY, Liu YQ, Zhang Y, Jiao L, and Yang BF
- Subjects
- Animals, Humans, Mice, Bone Morphogenetic Proteins metabolism, Chemokine CXCL12 drug effects, Chemokine CXCL12 metabolism, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 metabolism, Neovascularization, Physiologic, Vascular Endothelial Growth Factor A drug effects, Vascular Endothelial Growth Factor A metabolism, Recombinant Proteins metabolism, Recombinant Proteins therapeutic use, Hypoxia-Inducible Factor 1, alpha Subunit drug effects, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Diabetes Mellitus, Experimental complications, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Experimental metabolism, Endothelial Progenitor Cells metabolism, Endothelial Progenitor Cells pathology, Growth Differentiation Factors therapeutic use, Growth Differentiation Factors metabolism, Wound Healing drug effects
- Abstract
Non-healing diabetic wounds (DW) are a serious clinical problem that remained poorly understood. We recently found that topical application of growth differentiation factor 11 (GDF11) accelerated skin wound healing in both Type 1 DM (T1DM) and genetically engineered Type 2 diabetic db/db (T2DM) mice. In the present study, we elucidated the cellular and molecular mechanisms underlying the action of GDF11 on healing of small skin wound. Single round-shape full-thickness wound of 5-mm diameter with muscle and bone exposed was made on mouse dorsum using a sterile punch biopsy 7 days following the onset of DM. Recombinant human GDF11 (rGDF11, 50 ng/mL, 10 μL) was topically applied onto the wound area twice a day until epidermal closure (maximum 14 days). Digital images of wound were obtained once a day from D0 to D14 post-wounding. We showed that topical application of GDF11 accelerated the healing of full-thickness skin wounds in both type 1 and type 2 diabetic mice, even after GDF8 (a muscle growth factor) had been silenced. At the cellular level, GDF11 significantly facilitated neovascularization to enhance regeneration of skin tissues by stimulating mobilization, migration and homing of endothelial progenitor cells (EPCs) to the wounded area. At the molecular level, GDF11 greatly increased HIF-1ɑ expression to enhance the activities of VEGF and SDF-1ɑ, thereby neovascularization. We found that endogenous GDF11 level was robustly decreased in skin tissue of diabetic wounds. The specific antibody against GDF11 or silence of GDF11 by siRNA in healthy mice mimicked the non-healing property of diabetic wound. Thus, we demonstrate that GDF11 promotes diabetic wound healing via stimulating endothelial progenitor cells mobilization and neovascularization mediated by HIF-1ɑ-VEGF/SDF-1ɑ pathway. Our results support the potential of GDF11 as a therapeutic agent for non-healing DW., (© 2022. The Author(s), under exclusive licence to Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Chinese Pharmacological Society.)
- Published
- 2023
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156. Predicting willingness to donate blood based on machine learning: two blood donor recruitments during COVID-19 outbreaks.
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Wu HY, Li ZG, Sun XK, Bai WM, Wang AD, Ma YC, Diao RH, Fan EY, Zhao F, Liu YQ, Hong YZ, Guo MH, Xue H, and Liang WB
- Subjects
- Humans, Machine Learning, Intention, Disease Outbreaks, Blood Donors, COVID-19 epidemiology
- Abstract
Machine learning methods are a novel way to predict and rank donors' willingness to donate blood and to achieve precision recruitment, which can improve the recruitment efficiency and meet the challenge of blood shortage. We collected information about experienced blood donors via short message service (SMS) recruitment and developed 7 machine learning-based recruitment models using PyCharm-Python Environment and 13 features which were described as a method for ranking and predicting donors' intentions to donate blood with a floating number between 0 and 1. Performance of the prediction models was assessed by the Area under the receiver operating characteristic curve (AUC), accuracy, precision, recall, and F1 score in the full dataset, and by the accuracy in the four sub-datasets. The developed models were applied to prospective validations of recruiting experienced blood donors during two COVID-19 pandemics, while the routine method was used as a control. Overall, a total of 95,476 recruitments via SMS and their donation results were enrolled in our modelling study. The strongest predictor features for the donation of experienced donors were blood donation interval, age, and donation frequency. Among the seven baseline models, the eXtreme Gradient Boosting (XGBoost) and Support vector machine models (SVM) achieved the best performance: mean (95%CI) with the highest AUC: 0.809 (0.806-0.811), accuracy: 0.815 (0.812-0.818), precision: 0.840 (0.835-0.845), and F1 score of XGBoost: 0.843 (0.840-0.845) and recall of SVM: 0.991 (0.988-0.994). The hit rate of the XGBoost model alone and the combined XGBoost and SVM models were 1.25 and 1.80 times higher than that of the conventional method as a control in 2 recruitments respectively, and the hit rate of the high willingness to donate group was 1.96 times higher than that of the low willingness to donate group. Our results suggested that the machine learning models could predict and determine the experienced donors with a strong willingness to donate blood by a ranking score based on personalized donation data and demographical details, significantly improve the recruitment rate of blood donors and help blood agencies to maintain the blood supply in emergencies., (© 2022. The Author(s).)
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- 2022
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157. Electroacupuncture Attenuates Post-Inflammatory IBS-Associated Visceral and Somatic Hypersensitivity and Correlates With the Regulatory Mechanism of Epac1-Piezo2 Axis.
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Guo J, Chen L, Wang YH, Song YF, Zhao ZH, Zhao TT, Lin ZY, Gu DM, Liu YQ, Peng YJ, Pei LX, and Sun JH
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- Animals, Hyperalgesia etiology, Hyperalgesia metabolism, Hyperalgesia therapy, Mice, Serotonin metabolism, Electroacupuncture, Guanine Nucleotide Exchange Factors genetics, Ion Channels genetics, Irritable Bowel Syndrome therapy
- Abstract
Electroacupuncture (EA) is considered to have a therapeutic effect in the relief of irritable bowel syndrome (IBS)-associated visceral hypersensitivity via the reduction of the level of 5-hydroxytryptamine (5-HT) and 5-HT
3 receptors (5-HT3 R). However, whether Epac1/Piezo2, as the upstream of 5-HT, is involved in this process remains unclear. We investigated whether EA at the ST36 and ST37 acupoints alleviated visceral and somatic hypersensitivity in a post-inflammatory IBS (PI-IBS) model mice via the Epac1-Piezo2 axis. In this study, we used 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced PI-IBS as a mouse model. Visceral sensitivity was assessed by the abdominal withdrawal reflex test. Somatic sensitivity was evaluated by the hind paw withdrawal threshold. Quantitative real-time PCR, immunofluorescence staining, ELISA, and Western blotting were performed to examine the expressions of Epac1, Piezo2, 5-HT, and 5-HT3 R from the mouse distal colon/L5-S2 dorsal root ganglia (DRG). Our results showed that EA improved the increased visceral sensation and peripheral mechanical hyperalgesia in PI-IBS model mice, and the effects of EA were superior to the sham EA. EA significantly decreased the protein and mRNA levels of Epac1 and Piezo2, and reduced 5-HT and 5-HT3 R expressions in the distal colon. Knockdown of colonic Piezo2 eliminated the effect of EA on somatic hypersensitivity. Combined knockdown of colonic Epac1 and Piezo2 synergized with EA in relieving visceral hypersensitivity and blocked the effect of EA on somatic hypersensitivity. Additionally, protein levels of Epac1 and Piezo2 were also found to be decreased in the L5-S2 DRGs after EA treatment. Taken together, our study suggested that EA at ST36 and ST37 can alleviate visceral and somatic hypersensitivity in PI-IBS model mice, which is closely related to the regulation of the Epac1-Piezo2 axis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Guo, Chen, Wang, Song, Zhao, Zhao, Lin, Gu, Liu, Peng, Pei and Sun.)- Published
- 2022
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158. Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production.
- Author
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Du F, Liu YQ, Xu YS, Li ZJ, Wang YZ, Zhang ZX, and Sun XM
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- Genetic Vectors, Membrane Transport Proteins genetics, Protein Transport, Recombinant Proteins genetics, Cloning, Molecular methods, DNA-Directed RNA Polymerases genetics, Escherichia coli genetics, Gene Expression Regulation, Bacterial, Recombinant Proteins biosynthesis, Viral Proteins genetics
- Abstract
Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (P
lacUV5 ), which is leakier and more active than wild-type lac promoter (PlacWT ) under certain growth conditions. These characteristics are not advantageous for the production of those recombinant proteins with toxic or growth-burdened. On the one hand, leakage expression of T7 RNAP leads to rapid production of target proteins under non-inducing period, which sucks resources away from cellular growth. Moreover, in non-inducing or inducing period, high expression of T7 RNAP production leads to the high-production of hard-to-express proteins, which may all lead to loss of the expression plasmid or the occurrence of mutations in the expressed gene. Therefore, more BL21 (DE3)-derived variant strains with rigorous expression and different expression level of T7 RNAP should be developed. Hence, we replaced PlacUV5 with other inducible promoters respectively, including arabinose promoter (ParaBAD ), rhamnose promoter (PrhaBAD ), tetracycline promoter (Ptet ), in order to optimize the production of recombinant protein by regulating the transcription level and the leakage level of T7 RNAP. Compared with BL21 (DE3), the constructed engineered strains had higher sensitivity to inducers, among which rhamnose and tetracycline promoters had the lowest leakage ability. In the production of glucose dehydrogenase (GDH), a protein that causes host autolysis, the engineered strain BL21 (DE3::ara) exhibited higher biomass, cell survival rate and foreign protein expression level than that of BL21 (DE3). In addition, these engineered strains had been successfully applied to improve the production of membrane proteins, including E. coli cytosine transporter protein (CodB), the E. coli membrane protein insertase/foldase (YidC), and the E. coli F-ATPase subunit b (Ecb). The engineered strains constructed in this paper provided more host choices for the production of recombinant proteins., (© 2021. The Author(s).)- Published
- 2021
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159. Asymmetric Reductive Amination/Ring-Closing Cascade: Direct Synthesis of Enantioenriched Biaryl-Bridged NH Lactams.
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Zhang Y, Liu YQ, Hu L, Zhang X, and Yin Q
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- Amination, Catalysis, Lactams chemistry, Molecular Structure, Amides chemistry, Lactams chemical synthesis
- Abstract
We report here a Ru-catalyzed enantioselective synthesis of biaryl-bridged NH lactams through asymmetric reductive amination and a spontaneous ring-closing cascade from keto esters and NH
4 OAc with H2 as reductant. The reaction features broad substrate generality and high enantioselectivities (up to >99% ee). To showcase the practical utility, a highly enantioselective synthesis of 5-ethylindolobenzazepinone C , a promising antimitotic agent, has been rapidly completed. Furthermore, the amide group in the products enables versatile elaborations through directed C-H functionalization.- Published
- 2020
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160. The urinary levels of CTX-II, C2C, PYD, and Helix-II increased among adults with KBD: a cross-sectional study.
- Author
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Song QQ, Sun LY, Li CH, Liu YJ, Cui SL, Liu YQ, Cao YH, Pei JR, Wang Y, Lian W, Jiao Z, Deng Q, and Yu J
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- Adult, Aged, Biomarkers urine, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Amino Acids urine, Collagen Type II urine, Kashin-Beck Disease diagnosis, Kashin-Beck Disease urine, Peptide Fragments urine
- Abstract
Background: Kashin-Beck disease (KBD) is an endemic osteoarthropathy, and its pathogenesis is still not entirely clear. Pathologically, many KBD changes are similar to those of osteoarthritis (OA). Therefore, this study aimed to identify changes in the levels of potential urinary biomarkers for OA, including C-telopeptide of type II collagen (uCTX-II), type II collagen cleavage neoepitope (uC2C), pyridinoline (uPYD), and uHelix-II, among adults with KBD., Methods: Urinary samples of 83 external control (EC) subjects, 91 KBD patients, and 86 internal control (IC) subjects were tested by ELISA after the subjects completed a questionnaire and X-ray examination., Results: The medians of the four markers in the KBD group were higher than those in the EC group and those in the IC group. The medians in the grade II KBD group were higher than those in the grade I group but were not statistically significant (P = 0.301, P = 0.408, P = 0.204, and P = 0.898 for uCTX-II, uC2C, uPYD, and uHelix-II, respectively). The area under the curve (AUC) of uCTX-II (0.775) was higher than that of the others (0.672, 0.639, and 0.628 for uC2C, uPYD, and uHelix-II, respectively)., Conclusion: The levels of uCTX-II, uC2C, uPYD, and uHelix-II were elevated in adults with KBD and showed an increasing trend as the severity of KBD increased. The prediction accuracy of uCTX-II was more useful than that of the others for assisting in the diagnosis of KBD.
- Published
- 2019
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161. Modified polyoxometalate: a novel monocapped bi-supporting and reduced α-Keggin structure {PMo 12 O 40 [Cu(2,2'-bpy)]}[Cu(2,2'-bpy)(en)(H 2 O)] 2 .
- Author
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Lu YK, Li YP, Yang LY, Wang WH, Pan Y, Yan WF, and Liu YQ
- Abstract
A novel modified polyoxometalate, {PMo
12 O40 [Cu(2,2'-bpy)]}[Cu(2,2'-bpy)(en)(H2 O)]2 [2,2'-bpy is 2,2'-bipyridyl (C10 H8 N2 ) and en is ethylenediamine (C2 H8 N2 )], has been synthesized hydrothermally and structurally characterized by elemental analysis, TG, IR, XPS and single-crystal X-ray diffraction. The structural analysis reveals that the compound contains the reduced Keggin polyanion [PMo12 O40 ]6- as the parent unit, which is monocapped by [Cu(2,2'-bpy)]2+ fragments via four bridging O atoms on an {Mo4 O4 } pit and bi-supported by two [Cu(2,2'-bpy)(en)(H2 O)]2+ coordination cations simultaneously. There exist strong intramolecular π-π stacking between the capping and supporting units, which play a stabilizing role during the crystallization of the compound. Adjacent POM clusters are further aggregated to form a three-dimensional supramolecular network through noncovalent forces, hydrogen bonding and π-π stacking interactions. In addition, the photocatalytic properties were investigated in detail, and the results indicated that the compound can be used as a photocatalyst towards the decomposition of the organic pollutant methylene blue (MB).- Published
- 2019
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162. MicroRNA-132 regulates total protein of Nav1.1 and Nav1.2 in the hippocampus and cortex of rat with chronic cerebral hypoperfusion.
- Author
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Hu XL, Wang XX, Zhu YM, Xuan LN, Peng LW, Liu YQ, Yang H, Yang C, Jiao L, Hang PZ, and Sun LH
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- Animals, Brain Ischemia metabolism, Brain Ischemia pathology, Cerebral Cortex blood supply, Cerebral Cortex pathology, Cerebrovascular Circulation physiology, Cognitive Dysfunction metabolism, Cognitive Dysfunction pathology, Dementia metabolism, Dementia pathology, Disease Models, Animal, HEK293 Cells, Hippocampus blood supply, Hippocampus pathology, Humans, Male, MicroRNAs genetics, NAV1.2 Voltage-Gated Sodium Channel genetics, Neurodegenerative Diseases metabolism, Neurodegenerative Diseases pathology, Neurons metabolism, Neurons pathology, Rats, Rats, Sprague-Dawley, Temporal Lobe pathology, Cerebral Cortex metabolism, Hippocampus metabolism, MicroRNAs metabolism, NAV1.1 Voltage-Gated Sodium Channel genetics, NAV1.1 Voltage-Gated Sodium Channel metabolism, NAV1.2 Voltage-Gated Sodium Channel metabolism
- Abstract
Nav1.1 and Nav1.2 are the voltage-gated sodium channel alpha subunit1 and 2, encoded by the genes of SCN1A and SCN2A. Previous studies have shown that chronic cerebral hypoperfusion (CCH) could induce neuropathological and cognitive impairment and increased total Nav1.1 and Nav1.2protein levels, yet the detailed mechanisms are not fully understood. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are involved in the regulation of dementia. miR-132 is known to play a key role in neurodegenerative disease. Here, we determined that miR-132 regulates Nav1.1 and Nav1.2 under CCH state. In this study, the expression of miR-132 was decreased in both the hippocampus and cortex of ratsfollowing CCH generated by bilateral common carotid artery occlusion (2VO). Lentiviral-mediated overexpression of miR-132 ameliorated dementia vulnerability induced by 2VO. At the molecular level, miR-132 repressed the increased protein expression of Nav1.1 and Nav1.2 in both the hippocampus and cortex induced by 2VO. MiR-132 suppressed, while AMO-miR-132 enhanced, the level of Nav1.1 and Nav1.2 in primary cultured neonatal rat neurons (NRNs) detected by both western blot analysis and immunofluorescence analysis. Results obtained by dual luciferase assay showed that overexpression of miR-132 inhibited the expression of Nav1.1 and Nav1.2 in human embryonic kidney 293 (HEK293T) cells. Additionally, binding-site mutation failed to influence Nav1.1 and Nav1.2, indicating that Nav1.1 and Nav1.2 are potential targets for miR-132. Taken together, our findings demonstrated that miR-132 protects against CCH-induced learning and memory impairments by down-regulating the expression of Nav1.1 and Nav1.2, and SCN1A and SCN2A are the target genes of miR-132., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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163. Serum levels of PIICP, PIIANP, and PIIBNP are decreased in patients with an endemic osteochondropathy, Kashin-Beck disease.
- Author
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Lian W, Liu H, Sun LY, Liu YQ, Cui SL, Wang Y, Song QQ, Deng Q, Wang SP, Cao YH, Zhang XY, Jiang YY, Lv HY, Duan LB, and Yu J
- Subjects
- Aged, Biomarkers blood, China epidemiology, Female, Humans, Kashin-Beck Disease epidemiology, Male, Middle Aged, Osteochondrosis blood, Osteochondrosis diagnostic imaging, Osteochondrosis epidemiology, Collagen Type II blood, Kashin-Beck Disease blood, Kashin-Beck Disease diagnostic imaging, Procollagen blood
- Abstract
Background: Kashin-Beck disease (KBD) is an endemic, chronic, degenerative osteoarthropathy. KBD is usually diagnosed by using X-ray image and clinical symptoms, lacking of serological biomarkers. The serum level of PIICP, PIIANP, and PIIBNP can specifically reflect the damage of the cartilage. So, in this study, the serum levels of PIICP, PIIANP, and PIIBNP were detected in order to determine whether they can be used as potential biomarkers for the diagnosis of KBD., Method: Using a status survey, the survey sites were selected in the KBD historical endemic areas and non-endemic areas in Jilin and Heilongjiang provinces. All local residents have undergone clinical examination, X-ray examination of the hands and knees, and questionnaire survey. A total of 554 people were surveyed, and 184 residents who are eligible for inclusion criteria were selected as our subjects. Fifty-six cases were diagnosed as KBD and 63 individuals were included as internal control and 65 subjects were included as external control. And blood samples of surveyed subjects were collected, and the serum was separated to detect the levels of PIICP, PIIANP, and PIIBNP by ELISA. Statistical analysis was performed using the SPSS software., Results: There were no statistically significant differences in age and sex among the three groups. The Kruskal-Wallis H test showed that the serum levels of PIICP, PIIANP, and PIIBNP were significantly different among the three groups. Multiple comparisons using Dunnett's T3 test revealed that serum levels of PIICP, PIIANP, and PIIBNP were significantly lower in KBD patients than in internal and external control. However, there was no significant difference between the internal and external control., Conclusions: The results preliminarily indicated that the levels of PIICP, PIIANP, and PIIBNP in serum could reflect the abnormal synthesis of type II collagen in KBD patients and suggested that these indicators might be used as potential biomarkers for the diagnosis of KBD.
- Published
- 2018
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164. Hyperbaric oxygen preconditioning inhibits skin flap apoptosis in a rat ischemia-reperfusion model.
- Author
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Xiao YD, Liu YQ, Li JL, Ma XM, Wang YB, Liu YF, Zhang MZ, Zhao PX, Xie F, and Deng ZX
- Subjects
- Animals, Caspase 3 metabolism, Disease Models, Animal, MAP Kinase Kinase Kinase 5 metabolism, Male, Proto-Oncogene Proteins c-bcl-2 metabolism, Random Allocation, Rats, Sprague-Dawley, Reperfusion Injury metabolism, Reperfusion Injury pathology, Skin metabolism, Skin pathology, bcl-2-Associated X Protein metabolism, Apoptosis, Hyperbaric Oxygenation, Ischemic Preconditioning methods, Reperfusion Injury prevention & control, Skin blood supply
- Abstract
Background: Hyperbaric oxygen (HBO) improves skin flap function and inhibits partial necrosis induced by ischemia-reperfusion (I/R) injury. Our study aimed to evaluate the mechanism underlying HBO regulation of the antiapoptosis factors associated with I/R injury of skin flaps., Methods: The rats were divided into sham surgery, I/R, and HBO groups. Rats from the HBO group received HBO preconditioning followed by I/R surgery. Blood perfusion of the skin flaps was measured with laser Doppler flowmeters. Tissue morphology and apoptosis were subsequently assessed based on hematoxylin-eosinhe and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining. Protein expression of phosphorylated apoptosis signal-regulating kinase 1 (pASK-1), phosphorylated c-Jun N-terminal kinase (pJNK), B-cell lymphoma-2 (Bcl-2), and Bcl2-associated X protein (Bax) was examined by immunodetection, and Bcl-2 messenger RNA expression was detected by quantitative polymerase chain reaction. In addition, caspase-3 activity was also measured., Results: The result of microcirculation analysis showed that the survival and blood perfusion rates significantly increased in the skin flap after HBO exposure. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining revealed that cell apoptosis was significantly attenuated in the HBO group. Furthermore, HBO preconditioning increased the expression of Bcl-2 and inhibited pASK-1, pJNK, and Bax expression as determined by both immunohistochemistry and Western blot. Caspase-3 activity and the Bax/Bcl-2 ratio declined in the HBO group., Conclusions: HBO preconditioning effectively ameliorates I/R injury by regulating the apoptosis signal-regulating kinase 1 and/or c-Jun N-terminal kinase pathway and anti- and proapoptosis factors., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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165. Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway.
- Author
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Liu YQ, Liu YF, Ma XM, Xiao YD, Wang YB, Zhang MZ, Cheng AX, Wang TT, Li JL, Zhao PX, Xie F, and Zhang X
- Subjects
- Animals, Apoptosis genetics, Free Tissue Flaps blood supply, MAP Kinase Kinase Kinase 5 metabolism, Male, Rats, Rats, Sprague-Dawley, bcl-2-Associated X Protein metabolism, Apoptosis drug effects, Hydrogen administration & dosage, Ischemia prevention & control, Proto-Oncogene Proteins c-bcl-2 metabolism, Reperfusion Injury prevention & control, Saline Solution, Hypertonic administration & dosage, Skin blood supply
- Abstract
Introduction: Many pathways have been reported involving the effect of hydrogen-rich saline on protecting skin flap partial necrosis induced by the inflammation of ischemia/reperfusion injury. This study focused on the influence of hydrogen-rich saline treatment on apoptosis pathway of ASK-1/JNK and Bcl-2/Bax radio in I/R injury of skin flaps., Methods: Adult male Sprague-Dawley rats were divided into three groups. Group 1 was sham surgery group, Group 2 and 3 were ischemia/reperfusion surgery treated with physiological saline and hydrogen-rich saline respectively. Blood perfusion of flap was measured by Laser doppler flowmeters. Hematoxylin and eosin staining was used to observe morphological changes. Early apoptosis in skin flap was observed through TUNEL staining and presented as the percentage of TUNEL-positive cells of total cells. pASK-1, pJNK, Bcl-2 and Bax were examined by immunodetection. In addition Bcl-2, Bax and caspase-3 were detected by qPCR. Caspase-3 activity was also measured., Results: Compared to the Group 2, tissues from the group 3 were observed with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis., Conclusion: The results indicate that hydrogen-rich saline could ameliorate ischemia/reperfusion injury and improve flap survival rate by inhibiting the apoptosis factor and, at the same time, promoting the expression of anti-apoptosis factor., (Copyright © 2015. Published by Elsevier Ltd.)
- Published
- 2015
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166. μ(3)-Dodeca-tungsto(V,VI)aluminato-κO:O':O''-tris-[aqua-bis-(ethyl-ene-diamine-κN,N')copper(II)].
- Author
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Lu YK, Qu YY, Tian MM, Diao CL, and Liu YQ
- Abstract
The title compound, [AlCu(3)W(12)O(40)(C(2)H(8)N(2))(6)(H(2)O)(3)], was prepared under hydro-thermal conditions. The Cu(2+) ion displays an elongated octa-hedral geometry defined by one bridging O atom from the polyoxidoanion and a coordinated water mol-ecule in axial positions and four N atoms of the two chelating ethyl-enediamine (en) ligands in equatorial positions. The one-electron reduced [AlW(12)O(40)](6-) anion coordinates three [Cu(en)(H(2)O)](2+) fragments, generating a neutral tri-supported Keggin-type polyoxidometalate (POM). This tri-supported POM is located in a special position of [Formula: see text] symmetry and therefore O atoms from the central AlO(4) tetra-hedron are disordered over two sets of sites. Disorder is also observed for three other bridging O atoms of the POM. In the crystal, mol-ecules are connected via N-H⋯O and O-H⋯O hydrogen bonds, forming a three-dimensional framework.
- Published
- 2011
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167. Benzyl 5-phenyl-pyrazolo-[5,1-a]isoquino-line-1-carboxyl-ate.
- Author
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Lu YK, Yao X, Luo LW, Lü RQ, and Liu YQ
- Abstract
In the title compound, C(25)H(18)N(2)O(2), the pyrazolo-[5,1-a]iso-quin-oline ring system is approximately planar [maximum deviation = 0.027 (2) Å] and is oriented at dihedral angles of 57.22 (6) and 71.36 (7)° with respect to the two phenyl rings. The phenyl rings are twisted to each other by a dihedral angle of 66.33 (8)°. A weak intra-molecular C-H⋯O hydrogen bond occurs. In the crystal, weak C-H⋯π inter-actions are present.
- Published
- 2011
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168. Poly[[(4,4'-bipyridine-κN)[μ(3)-(S)-2-hy-droxy-butane-dioato-κO,O:O:O]zinc] dihydrate].
- Author
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Lu YK, Liu J, Diao CL, Lü RQ, and Liu YQ
- Abstract
In the title compound, {[Zn(C(4)H(4)O(5))(C(10)H(8)N(2))]·2H(2)O}(n), the Zn(II) ion displays a distorted tetra-gonal-pyramidal coordination environment with one hy-droxy O and three carboxyl-ate O atoms from three malate anions, and the one remaining position occupied by an N atom from a 4,4'-bipyridine ligand. The pyridine rings of the 4,4'-bipyridine ligand are twisted with respect to each other by a dihedral angle of 35.8 (2)°. The uncoordinated water mol-ecules are linked to the complex mol-ecules by O-H⋯O hydrogen bonds. Each malate anion forms four coordination bonds with three Zn atoms, establishing a layer structure parallel to the ac plane. Adjacent layers are further linked via O-H⋯N hydrogen bonding. π-π stacking between the pyridine rings [face-to-face distance = 3.651 (3) Å] occurs in the crystal structure.
- Published
- 2011
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169. Bis[tris-(ethyl-enediamine-κN,N')cobalt(III)] octa-kis-μ-(3)-oxido-hexa-deca-μ(2)-oxido-tetra-deca-oxido-μ(12)-tetra-oxo-silicato-octa-molybdenum(VI)hexa-vanadium(IV,V) hexa-hydrate.
- Author
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Lu YK, Tian MM, Xu SG, Lü RQ, and Liu YQ
- Abstract
The title compound, [Co(C(2)H(8)N(2))(3)](2)[SiMo(8)V(4)O(40)(VO)(2)]·6H(2)O, was prepared under hydro-thermal conditions. The asymmetric unit consists of a transition metal complex [Co(en)(3)](3+) cation (en is ethyl-enediamine), one half of an [SiMo(8)V(4)O(40)(VO)(2)](6-) heteropolyanion, two solvent water mol-ecules in general positions and two half-mol-ecules of water located on a mirror plane. In the complex cation, the Co(3+) ion is in a distorted octa-hedral coordination environment formed by six N atoms of the three chelating en ligands. One of the en ligands exhibits disorder of its aliphatic chain over two sets of sites of equal occupancy. The [SiMo(8)V(4)O(40)(VO)(2)](6-) heteropolyanion is a four-electron reduced bivanadyl-capped α-Keggin-type molybdenum-vanadium-oxide cluster. In the crystal, it is located on a mirror plane, which results in disorder of the central tetra-hedral SiO(4) group: the O atoms of this group occupy two sets of sites related by a mirror plane. Furthermore, all of the eight μ(2)-oxide groups are also disordered over two sets of sites with equal occupancy. There are extensive inter-molecular N-H⋯O hydrogen bonds between the complex cations and inorganic polyoxidoanions, leading to a three-dimensional supra-molecular network.
- Published
- 2011
- Full Text
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170. [XPS spectra of Langmuir-Blodgett films and their electroluminescence].
- Author
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Ouyang JM, Bai Y, Yu G, Wang S, Liu YQ, Li YF, and Zhu DB
- Abstract
The coordination of monolayer of amphiphilic ligands, N, N-bis(8-quinolinyl)alkyl propanediamide, H2A(alkyl = dodecyl, H2A12; hexadecyl, H2A16) with Cu2+ at air-water interface was investigated by X-ray Photoelectron Spectroscopy (XPS) and UV-Vis spectra. The collapse pressure of the monolayers of H2A12 and H2A16 increased from 12.5 and 15.6 mN x m(-1) to 28.0 and 33.8 mN x m(-1) after their coordination with Cu2+ ions, respectively. XPS indicated that the coordination ratio between H2A and Cu2+ was 1:1. The binding energies of Cu2p were 935 and 955 eV. The Langmuir-Blodgett(LB) films of H2A12 and H2A16 can be used as transporting materials in three-layer electroluminescence devices: ITO/TPD/LB films/Alq3/Al. The driving voltages were 6.5 and 7.5 V, the maximum luminances were 621 and 201 cd x m(-2), respectively.
- Published
- 2004
171. Preparation and Characterization of Novel Amphiphilic C(60) Derivatives.
- Author
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Zhu CC, Xu Y, Liu YQ, and Zhu DB
- Abstract
Amphiphilic C(60) derivatives of [(ethoxycarbonyl)decylene]fullerene and bis[(ethoxycarbonyl)decylene]fullerene, which are used to obtain organized molecular films by the LB technique, were prepared by the reaction of C(60) with diazo compounds. They were characterized by IR, UV-vis, (1)H-NMR, (13)C-NMR, and FD-MS. Direct evidence confirmed that the [6,5]-fullerenoid is the thermodynamically more stable isomer. It was converted from the arrangement of a [6,6]methanofullerene rather than a reaction product in 6,5 double bonds. A carbene mechanism is suggested for the reaction.
- Published
- 1997
- Full Text
- View/download PDF
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