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Achromobacter spanius strain 6 is a Gram-negative soil bacterium isolated from a hydrocarbon-degrading microcosm. The draft genome sequence of A. spanius strain 6 is 6.57 Mb with a G+C content of 64.7% and 5,855 protein coding genes., Achromobacter spanius strain 6 is a Gram-negative soil bacterium isolated from a hydrocarbon-degrading microcosm. The draft genome sequence of A. spanius strain 6 is 6.57 Mb with a G+C content of 64.7% and 5,855 protein coding genes. Multiple genes involved in degradation of aromatics are present in this strain.

High levels of religious involvement have been associated with positive mental health outcomes in older adults. This study investigated whether the effects of religion on healthy aging could generalize to older LGBTQ adults.This study examined religious affiliation in childhood versus at present, as well as the relationships of outness to religious community and religious engagement with depression and loneliness in 102 lesbian, gay, and bisexual cisgender, transgender, and questioning adults (LGBTQ) over the age of 55. As part of a larger study, participants completed several questions and measures including: one outness to religious community item, 4 religious engagement questions, the Center for Epidemiological Studies Depression Scale, and the 8-item UCLA Loneliness Scale.Many older LGBTQ adults reported changes in religious affiliation from childhood to adulthood. Greater levels of outness to religious communities and lower religious engagement were related to lower levels of depression and loneliness.Findings suggest that outness to religious communities may be beneficial to the mental health of older LGBTQ adults. Higher levels of distress may lead to higher levels of religious engagement.Religion, especially those that are supportive of sexual minorities, may help to improve the well-being of older LGBTQ adults.

This study summarizes the association between medical amnesty policy (MAP) implementation and drinking behaviors at a 4-year university.First-year students (N = 1,246; 50.8% male) were assessed in the academic year pre- (2009-2010, n = 571) and post-implementation (2010-2011, n = 675). A cohort-sequential design was used. Students were assessed at college entry and end-of-year. Self-report measures assessed recent alcohol use, physiological consequences, and helping behaviors specific to alcohol-related medical emergencies.MAP implementation did not increase drinking, overall consumption, or the incidence of physiological consequences. Modest increases in contacting residence life staff in the event of an emergency were also found.This study provides empirical support that MAP policies do not increase consumption or problems and may reduce barriers to seeking help in the event of an emergency. Additional research is needed to establish the effectiveness of MAPs as an environmental-level strategy to reduce harmful drinking on campus.

Multipoint linkage analysis is an important approach for localizing disease-associated loci in pedigrees. Linkage analysis, however, is sensitive to misspecification of marker allele frequencies. Pedigrees from recently admixed populations are particularly susceptible to this problem because of the challenge of accurately accounting for population structure. Therefore, increasing emphasis on use of multiethnic samples in genetic studies requires reevaluation of best practices, given data currently available. Typical strategies have been to compute allele frequencies from the sample, or to use marker allele frequencies determined by admixture proportions averaged over the entire sample. However, admixture proportions vary among pedigrees and throughout the genome in a family-specific manner. Here, we evaluate several approaches to model admixture in linkage analysis, providing different levels of detail about ancestral origin. To perform our evaluations, for specification of marker allele frequencies, we used data on 67 Caribbean Hispanic admixed families from the Alzheimer's Disease Sequencing Project. Our results show that choice of admixture model has an effect on the linkage analysis results. Variant-specific admixture proportions, computed for individual families, provide the most detailed regional admixture estimates, and, as such, are the most appropriate allele frequencies for linkage analysis. This likely decreases the number of false-positive results, and is straightforward to implement.

Purpose Earlier, we reported development and initial testing of a rapid, low burden measure of wheelchair seated posture (the Seated Posture Scale [SPS]) for research and clinical use. The purpose of this study was to test the intrarater reliability, interrater reliability, and criterion related validity of the SPS. Design We used a descriptive design to test and develop the instrument. Methods We used the method described by Walter, Donner, and Eliasziw to determine 46 participants were needed, from two Veterans Administration nursing homes or CLCs (community living centers). Using a digital goniometer to score rapidly and with two trained raters, we scored individual participants simultaneously and again in succession. For criterion related concurrent validity, we also scored each participant with Section 2 of the Seated Postural Control Measure for Adults. Intrarater reliability, interrater reliability, and criterion related, concurrent validity were assessed using kappa statistics for individual instrument items (and corresponding 95% confidence intervals where appropriate) and intraclass correlation coefficients (ICC) for total scores. Findings The intrarater intraclass correlation coefficient (ICC) was .995; interrater ICC was .80; interrater reliability kappas ranged from -.03 to .80. Criterion-related, concurrent validity kappas ranged from .13 to .91. ICC for total scores was .85. Conclusion The SPS has sufficient preliminary validity and reliability to support measurement of wheelchair-seated posture in outcomes research and clinical use. Clinical relevance In the future, rehabilitation nurses may use the SPS to improve management of wheelchair seated posture, to improve clinical outcomes, particularly for those who do not reposition themselves.

Objective Neuroendocrine tumors of the lung are currently staged with the 7th edition TNM non–small cell lung cancer staging system. This decision, based on data analysis without data on histology or disease-specific survival, makes its applicability limited. This study proposes a specific staging system for these tumors. Methods We retrospectively analyzed 510 consecutive patients (female/male, 313/197; median age, 61 years; interquartile range, 51-70) undergoing lung resection for a primary neuroendocrine tumor between 2000 and 2015 in 8 centers. Multivariable analysis was performed using a Cox proportional hazard model to identify factors associated with disease-specific survival. A new staging system was proposed on the basis of the results of this analysis. Kaplan–Meier disease-specific survival was analyzed by stage using the proposed and the 7th TNM staging system. Results Follow-up was completed in 490 of 510 patients at a median of 51 months (interquartile range, 18-99). Histology (G1-typical carcinoid vs G2-atypical carcinoid vs G3-large-cell neuroendocrine carcinoma) and pT were independently associated with survival, but pN was not. After regrouping histology and pT, we proposed the following staging system: IA (pT1-2G1), IB (pT3G1, pT1G2), IIA (pT4G1, pT2-3G2, pT1G3), IIB (pT4G2, pT2-3G3), and III (pT4G3). The 5-year survivals were 97.9%, 81.0%, 69.1%, 51.8%, and 0%, respectively. By using the 7th TNM, 5-year survivals were 95.0%, 92.3%, 67.7%, 70.9%, and 65.1% for stage IA, IB, IIA, IIB, and III, respectively. Conclusions Incorporating histology and regrouping tumor stage create a unique neuroendocrine tumor staging system that seems to predict survival better than the 7th TNM classification.

We present the Moments in Time Dataset, a large-scale human-annotated collection of one million short videos corresponding to dynamic events unfolding within three seconds. Modeling the spatial-audio-temporal dynamics even for actions occurring in 3 second videos poses many challenges: meaningful events do not include only people, but also objects, animals, and natural phenomena; visual and auditory events can be symmetrical in time (“opening” is “closing” in reverse), and either transient or sustained. We describe the annotation process of our dataset (each video is tagged with one action or activity label among 339 different classes), analyze its scale and diversity in comparison to other large-scale video datasets for action recognition, and report results of several baseline models addressing separately, and jointly, three modalities: spatial, temporal and auditory. The Moments in Time dataset, designed to have a large coverage and diversity of events in both visual and auditory modalities, can serve as a new challenge to develop models that scale to the level of complexity and abstract reasoning that a human processes on a daily basis.

Financial literacy is defined as “the ability to use knowledge and skills to manage financial resources effectively for a lifetime of financial well-being.” The Money Smart Peer-to-Peer Program (MSP) was developed by the FDIC for use with diverse populations. MSP was culturally adapted for use in Jamaica using the Johns Hopkins DIME process. Peer counselling programs consistently demonstrate that they are acceptable and effective with adults who have limited education and low-income by overcoming barriers that are typically encountered when people attempt to access unfamiliar services or guidance from a professional. Research conducted with the MSP program reveals that program participants were more likely to open and save using deposit accounts, use and adhere to a budget, and have increased confidence in their financial abilities 6 to 12 months after completing the course. Implementation of MSP increases the likelihood that people can retire with dignity and financial security.

Understanding the effect of conventional and alternative fuels on the marine bacterial community is crucial, as it pertains to the impact, biodegradation, and final fate of these fuels in the environment. Metagenomics analysis demonstrated that conventional and alternative fuels promoted the growth of Proteobacteria. Marinobacter and Desulfovibrio were predominant in seawater exposed to conventional jet propellant-5 (JP-5), while Hyphomonas and Rhodovulum were most abundant in seawater with hydroprocessed renewable jet fuel (HRJ) and conventional F-76 diesel, respectively. The phyla Bacteroidetes, Firmicutes, and Lentisphaerae were underrepresented in samples with fuel, and these phyla were largely comprised of unclassified bacteria. Culture-dependent tests isolated several of the same genera detected in high abundance by metagenomics DNA sequencing, including Marinobacter, Rhodovulum, and Halobacillus. Growth studies in fuel and gas chromatography analysis demonstrated that isolates grew in fuel and meta...

The African elephant consists of forest and savanna subspecies. Both subspecies are highly endangered due to severe poaching and habitat loss, and knowledge of their population structure is vital to their conservation. Previous studies have demonstrated marked genetic and morphological differences between forest and savanna elephants, and despite extensive sampling, genetic evidence of hybridization between them has been restricted largely to a few hybrids in the Garamba region of northeastern Democratic Republic of Congo (DRC). Here, we present new genetic data on hybridization from previously unsampled areas of Africa. Novel statistical methods applied to these data identify 46 hybrid samples--many more than have been previously identified--only two of which are from the Garamba region. The remaining 44 are from three other geographically distinct locations: a major hybrid zone along the border of the DRC and Uganda, a second potential hybrid zone in Central African Republic and a smaller fraction of hybrids in the Pendjari-Arli complex of West Africa. Most of the hybrids show evidence of interbreeding over more than one generation, demonstrating that hybrids are fertile. Mitochondrial and Y chromosome data demonstrate that the hybridization is bidirectional, involving males and females from both subspecies. We hypothesize that the hybrid zones may have been facilitated by poaching and habitat modification. The localized geography and rarity of hybrid zones, their possible facilitation from human pressures, and the high divergence and genetic distinctness of forest and savanna elephants throughout their ranges, are consistent with calls for separate species classification.

Extracellular vesicles (EVs) are produced by all domains of life. In Gram-negative bacteria, EVs are produced by the pinching off of the outer membrane; however, how EVs escape the thick cell walls of Gram-positive bacteria, mycobacteria and fungi is still unknown. Nonetheless, EVs have been described in a variety of cell-walled organisms, including Staphylococcus aureus, Mycobacterium tuberculosis and Cryptococcus neoformans. These EVs contain varied cargo, including nucleic acids, toxins, lipoproteins and enzymes, and have important roles in microbial physiology and pathogenesis. In this Review, we describe the current status of vesiculogenesis research in thick-walled microorganisms and discuss the cargo and functions associated with EVs in these species.

OBJECTIVE: This prospective longitudinal study aims to determine the risk factors of wandering-related adverse consequences in community-dwelling persons with mild dementia. These adverse consequences include negative outcomes of wandering (falls, fractures, and injuries) and eloping behavior. METHODS: We recruited 143 dyads of persons with mild dementia and their caregivers from a veteran's hospital and memory clinic in Florida. Wandering-related adverse consequences were measured using the Revised Algase Wandering Scale - Community Version. Variables such as personality (Big Five Inventory), behavioral response to stress, gait, and balance (Tinetti Gait and Balance), wayfinding ability (Wayfinding Effectiveness Scale), and neurocognitive abilities (attention, cognition, memory, language/verbal skills, and executive functioning) were also measured. Bivariate and logistic regression analyses were performed to assess the predictors of these wandering-related adverse consequences. RESULTS: A total of 49% of the study participants had falls, fractures, and injuries due to wandering behavior, and 43.7% demonstrated eloping behaviors. Persistent walking (OR = 2.6) and poor gait (OR = 0.9) were significant predictors of negative outcomes of wandering, while persistent walking (OR = 13.2) and passivity (OR = 2.55) predicted eloping behavior. However, there were no correlations between wandering-related adverse consequences and participants' characteristics (age, gender, race, ethnicity, and education), health status (Charlson comorbidity index), or neurocognitive abilities. CONCLUSION: Our results highlight the importance of identifying at-risk individuals so that effective interventions can be developed to reduce or prevent the adverse consequences of wandering. Copyright © 2015 John Wiley & Sons, Ltd. Language: en

The study examined Florida assisted living facility staff perspectives of disaster preparedness, response, and recovery after the 2004 and 2005 hurricanes. A descriptive study using qualitative and quantitative approaches. One hundred seventy Florida assisted living facility staff who worked in areas under hurricane threat and provided care to residents during a hurricane. A questionnaire that was mailed or administered to staff attending a regional planning conference with quantitative and qualitative items. Of the 143 facilities that were affected by a hurricane, 77 % reported that they sheltered residents in place, while 23 % indicated that they evacuated for one or more of the hurricanes. Almost one-third of facilities lost telephone service and almost half lost electrical power during one or more of the hurricanes. Key topics noted by both evacuating facilities and those that sheltered in place were 1) decision-making, 2) planning, support, and supplies, 3) communication, and 4) electricity. Unique to assisted living facilities is the role of residents’ family in assuming responsibility for evacuation and shelter during hurricanes. Facility staff at both evacuating and sheltering facilities indicated that the decision to evacuate or to shelter in place was difficult and the electrical power was critical to resident well-being. Assisted living facilities should develop disaster preparedness plans in conjunction with local and state emergency offices. Adequate disaster preparedness has implications for residents’ safety as well as assisted living facility staff during disasters.

Focused on protecting a few The illegal ivory trade threatens the persistence of stable wild elephant populations. The underground and covert nature of poaching makes it difficult to police. Wasser et al. used genetic tools to identify the origins of elephant tusks seized during transit (see the Perspective by Hoelzel). The majority of source animals were part of just a few wild elephant populations in Africa—and just two areas since 2006. Increased focus on enforcement in a few such areas could help interrupt poaching activities and restore wild elephant populations. Science , this issue p. 84 ; see also p. 34

In the Fluid and Catheter Treatment Trial (FACTT) of the National Institutes of Health Acute Respiratory Distress Syndrome Network, a conservative fluid protocol (FACTT Conservative) resulted in a lower cumulative fluid balance and better outcomes than a liberal fluid protocol (FACTT Liberal). Subsequent Acute Respiratory Distress Syndrome Network studies used a simplified conservative fluid protocol (FACTT Lite). The objective of this study was to compare the performance of FACTT Lite, FACTT Conservative, and FACTT Liberal protocols.Retrospective comparison of FACTT Lite, FACTT Conservative, and FACTT Liberal. Primary outcome was cumulative fluid balance over 7 days. Secondary outcomes were 60-day adjusted mortality and ventilator-free days through day 28. Safety outcomes were prevalence of acute kidney injury and new shock.ICUs of Acute Respiratory Distress Syndrome Network participating hospitals.Five hundred three subjects managed with FACTT Conservative, 497 subjects managed with FACTT Liberal, and 1,124 subjects managed with FACTT Lite.Fluid management by protocol.Cumulative fluid balance was 1,918 ± 323 mL in FACTT Lite, -136 ± 491 mL in FACTT Conservative, and 6,992 ± 502 mL in FACTT Liberal (p0.001). Mortality was not different between groups (24% in FACTT Lite, 25% in FACTT Conservative and Liberal, p = 0.84). Ventilator-free days in FACTT Lite (14.9 ± 0.3) were equivalent to FACTT Conservative (14.6 ± 0.5) (p = 0.61) and greater than in FACTT Liberal (12.1 ± 0.5, p0.001 vs Lite). Acute kidney injury prevalence was 58% in FACTT Lite and 57% in FACTT Conservative (p = 0.72). Prevalence of new shock in FACTT Lite (9%) was lower than in FACTT Conservative (13%) (p = 0.007 vs Lite) and similar to FACTT Liberal (11%) (p = 0.18 vs Lite).FACTT Lite had a greater cumulative fluid balance than FACTT Conservative but had equivalent clinical and safety outcomes. FACTT Lite is an alternative to FACTT Conservative for fluid management in Acute Respiratory Distress Syndrome.

Pseudomonas stutzeri strain 19 is a Gram-negative bacterium capable of degrading aromatic hydrocarbons. The draft genome of P. stutzeri 19 is estimated to be 5.1 Mb, containing 4,652 protein-coding genes and a G+C content of 63.3%. Multiple genes responsible for the degradation of aromatics are present in this strain.

Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive bacteria secreted extracellular vesicles (EVs) was not pursued due to the assumption that the thick peptidoglycan cell wall would prevent their release to the environment. However, following discovery in fungi, which also have cell walls, EVs have now been described for a variety of Gram-positive bacteria. EVs purified from Gram-positive bacteriaare implicated in virulence, toxin release and transference to host cells, eliciting immune responses, and spread of antibiotic resistance.Listeria monocytogenesis a Gram-positive bacterium that is the etiological agent of listeriosis. Here we report thatL. monocytogenesproduces EVs with diameter ranging from 20-200 nm, containing the pore-forming toxin listeriolysin O(LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC). Using simultaneousmetabolite,protein, andlipidextraction (MPLEx) multi-omics we characterized protein, lipid and metabolite composition of bacterial cells and secreted EVs and found that EVs carry the majority of listerial virulence proteins. Cell-free EV preparations were toxic to the murine macrophage cell line J774.16, in a LLO-dependent manner, evidencing EV biological activity. The deletion ofplcAincreased EV toxicity, suggesting PI-PLC can restrain LLO activity. Using immunogold electron microscopy we detect LLO localization at several organelles within infected human epithelial cells and with high-resolution fluorescence imaging we show that dynamic lipid structures are released fromL. monocytogenesthat colocalize with LLO during infection. Our findings demonstrate thatL. monocytogenesutilize EVs for toxin release and implicate these structures in mammalian cytotoxicity.

Lung cancer is the number one cause of cancer deaths. Many early stage lung cancer patients have resectable tumors; however, their cardiopulmonary function needs to be properly evaluated before they are deemed operative candidates. Consequently, a subset of such patients is asked to undergo standard pulmonary function tests, such as cardiopulmonary exercise tests (CPET) or stair climbs, to have their pulmonary function evaluated. The standard tests are expensive, labor intensive, and sometimes ineffective due to co-morbidities, such as limited mobility. Recovering patients would benefit greatly from a device that can be worn at home, is simple to use, and is relatively inexpensive. Using advances in information technology, the goal is to design a continuous, inexpensive, mobile and patient-centric mechanism for evaluation of a patient's pulmonary function. A light mobile mask is designed, fitted with CO2, O2, flow volume, and accelerometer sensors and tested on 18 subjects performing 15 minute exercises. The data collected from the device is stored in a cloud service and machine learning algorithms are used to train and predict a user's activity .Several classification techniques are compared - K Nearest Neighbor, Random Forest, Support Vector Machine, Artificial Neural Network, and Naive Bayes. One useful area of interest involves comparing a patient's predicted activity levels, especially using only breath data, to that of a normal person's, using the classification models.

As older persons make up an ever greater proportion of the world’s population, a range of concerns are being voiced by policy-makers, program managers, and care providers about best or optimal practices for serving this population’s needs during all stages of disasters. Given that age-related vulnerabilities are common in late life, this article describes existing systems of care in the United States for the provision of disaster mental health services. Second, it evaluates the evidence for disaster treatment interventions with this subgroup of the population. Third, it synthesizes the findings of recent studies focusing on screening, assessment, and treatment approaches. To advance our current system of care and to adequately respond to the mental health needs of older persons, it is advantageous to periodically review progress, identify current gaps and unmet needs, and describe opportunities for improvement. (Disaster Med Public Health Preparedness. 2018; 12: 366–372)

Disasters are catastrophic events that suddenly and adversely affect populations. International humanitarian support is routinely offered after disasters to alleviate human suffering and facilitate the recovery process. Policymakers and responders use their knowledge and experiences to develop and implement systematized strategies that can be followed during disasters. Yet despite these efforts, the rate of disaster-related morbidity and mortality for older adults remains significantly higher than the general population. Because disasters occur without warning it is difficult to test and enhance strategies and protocols that are intended to mitigate threats and hazards and to quickly and effectively resolve their negative consequences. By identifying persistent challenges encountered during disasters and considering issues facing an aging population, organizations responsible for disaster response may be better attuned to the changing needs of the population and more able to address older adults in their planning and training processes. This symposium will feature five speakers representing Cameroon, Jamaica, Japan, New Zealand, Nepal, Philippines, and the United States. Presentations will describe actual disasters and the challenges and opportunities for preparing and responding to older adults during disasters in countries with varied financial, material, and human resources. Older adults displaced during disasters, assistance during displacement, as well as during return and reintegration after the event will be described. Finally, we will discuss why so many identified problems remain unresolved over time and how disaster managers might adapt lessons learned into processes so that they are culturally sensitive, relevant, and useful in affected regions.

Depression as well as dissatisfaction with sexual wellbeing is common in older lesbian women and gay men. This study explored the relationship of sexual functioning and satisfaction with depression severity in lesbians and gay men who were ages 55 years and older. As part of a larger study, 32 lesbian women and 44 gay men were given the Center for Epidemiological Studies Depression Scale, Changes in Sexual Functioning Questionnaire, and questions regarding sexual satisfaction and frequency of current sexual activity. In gay men, depression severity was significantly correlated with sexual functioning (r=-.448, p=.015) but not with general sexual satisfaction (r=-.212, p=.287). When examining specific items of sexual satisfaction, enjoyment, frequency, desire, and thoughts and pleasure of thoughts of sexual activity were significantly negatively correlated with depression severity. As for sexual functioning, only ability and frequency of ejaculation and pleasure in orgasm was negatively correlated with depression severity in gay men. In lesbian women, depression was not significantly correlated with sexual functioning or satisfaction or to specific items of sexual satisfaction and functioning. Results suggest that depression severity was negatively correlated with sexual satisfaction but less so with functioning in gay men. However, no significant relationships were found between sexual functioning and satisfaction with depression in lesbian women. These findings suggest that sexual satisfaction and functioning may play an important role in the emotional wellbeing of older gay men, but not in older lesbian women.

Prior GWAS have identified loci associated with red blood cell (RBC) traits in populations of European, African, and Asian ancestry. These studies have not included individuals with an Amerindian ancestral background, such as Hispanics/Latinos, nor evaluated the full spectrum of genomic variation beyond single nucleotide variants. Using a custom genotyping array enriched for Amerindian ancestral content and 1000 Genomes imputation, we performed GWAS in 12,502 participants of Hispanic Community Health Study and Study of Latinos (HCHS/SOL) for hematocrit, hemoglobin, RBC count, RBC distribution width (RDW), and RBC indices. Approximately 60% of previously reported RBC trait loci generalized to HCHS/SOL Hispanics/Latinos, including African ancestral alpha- and beta-globin gene variants. In addition to the known 3.8kb alpha-globin copy number variant, we identified an Amerindian ancestral association in an alpha-globin regulatory region on chromosome 16p13.3 for mean corpuscular volume and mean corpuscular hemoglobin. We also discovered and replicated three genome-wide significant variants in previously unreported loci for RDW (SLC12A2 rs17764730, PSMB5 rs941718), and hematocrit (PROX1 rs3754140). Among the proxy variants at the SLC12A2 locus we identified rs3812049, located in a bi-directional promoter between SLC12A2 (which encodes a red cell membrane ion-transport protein) and an upstream anti-sense long-noncoding RNA, LINC01184, as the likely causal variant. We further demonstrate that disruption of the regulatory element harboring rs3812049 affects transcription of SLC12A2 and LINC01184 in human erythroid progenitor cells. Together, these results reinforce the importance of genetic study of diverse ancestral populations, in particular Hispanics/Latinos., Author summary Red blood cells (RBC) are important for transport of oxygen to tissues throughout the body. Distribution of RBC traits differs by ethnicity and gender, and both genetic and acquired factors likely contribute to these differences. Prior genetic studies have identified physical regions of the genome associated with RBC traits in populations with European, African, and Asian ancestry. These studies have not included individuals with ancestry from the American continents (Amerindian ancestry), such as Hispanics/Latinos. In an analysis of RBC traits in up to 19,608 Hispanics/Latinos, we identified an Amerindian-ancestry genetic association in a known alpha-globin regulatory region. We also identified three new RBC trait associations, including a regulatory variant of SLC12A2 that encodes a RBC membrane ion-transport protein. Experimental disruption of this regulatory element led to reduced expression of both SLC12A2 and an adjacent long non-coding RNA in human erythroid progenitor cells. These results contribute to understanding the physiology of red blood cells and reinforce the importance of genetic study of diverse ancestry populations, in particular Hispanics/Latinos.

Suicide among older adults and Veterans is increasingly recognized as a serious and rapidly growing public health problem. Older adults (age >65 per medicare), are more likely to die by suicide than any other adult age group, due to more lethal methods of suicide attempt. Suicide ideation and behaviors are the most common clinical emergency faced by mental health professionals. Despite sustained research efforts and the introduction of evidence-based practices into clinical practice, older individuals’ risk for suicide remains difficult to predict and treat, even with thorough assessment and competent intervention. US Department of Veterans Affairs. and Veterans Health Administration has designated Suicide Prevention Teams at all of its hospitals and is committed to the development and implementation of strategies designed to better identify and treat Veterans who are in emotional crisis and to the promotion of change in the environment, culture, and health care through clinical and public health programs that directly target suicide prevention.

Nocardioides luteus strain BAFB is a Gram-positive bacterium that efficiently degrades C 8 to C 11 alkanes aerobically. The draft genome of N. luteus BAFB is 5.76 Mb in size, with 5,358 coding sequences and 69.9% G+C content. The genes responsible for alkane degradation are present in this strain.

Circulating white blood cell (WBC) counts (neutrophils, monocytes, lymphocytes, eosinophils, basophils) differ by ethnicity. The genetic factors underlying basal WBC traits in Hispanics/Latinos are unknown. We performed a genome-wide association study of total WBC and differential counts in a large, ethnically diverse US population sample of Hispanics/Latinos ascertained by the Hispanic Community Health Study and Study of Latinos (HCHS/SOL). We demonstrate that several previously known WBC-associated genetic loci (e.g. the African Duffy antigen receptor for chemokines null variant for neutrophil count) are generalizable to WBC traits in Hispanics/Latinos. We identified and replicated common and rare germ-line variants at FLT3 (a gene often somatically mutated in leukemia) associated with monocyte count. The common FLT3 variant rs76428106 has a large allele frequency differential between African and non-African populations. We also identified several novel genetic loci involving or regulating hematopoietic transcription factors (CEBPE-SLC7A7, CEBPA and CRBN-TRNT1) associated with basophil count. The minor allele of the CEBPE variant associated with lower basophil count has been previously associated with Amerindian ancestry and higher risk of acute lymphoblastic leukemia in Hispanics. Together, these data suggest that germline genetic variation affecting transcriptional and signaling pathways that underlie WBC development and lineage specification can contribute to inter-individual as well as ethnic differences in peripheral blood cell counts (normal hematopoiesis) in addition to susceptibility to leukemia (malignant hematopoiesis).

Historically, a number of terms have been used to differentiate indirect self-destructive behaviours (ISDBs) from overt acts of suicide such as partial, focal and chronic suicide, sub-intentional death, deliberate self-harm and silent suicide. Each of these terms encompasses a wide range of behaviours such as: refusing to drink or eat, medication mismanagement, self-mutilation, self-neglect, and medical noncompliance. These behaviours all describe deleterious, life-threatening actions that ultimately undermine the health of the individual and lead to premature death. ISDBs are life threatening and commonly occurring, but poorly understood, behaviours that can mask suicidal intent. While many psychological, behavioural, and biological risk factors for completed suicide have been identified, the etiology, role, and clinical correlates of ISDBs have not been satisfactorily examined. In an attempt to reach common ground in describing subtypes of indirect self-destructive behaviour, the National Institute of Mental Health Mental (NIMH) convened 22 experts who defined an ISDB as “…an act of omission or commission that causes self-harm leading indirectly, over time, to the patient’s death”. Reports issued by the Institute of Medicine and by the NIMH of the Aging Research Branch noted that ISDBs are of major concern because they are highly prevalent and result in greater morbidity and mortality than more easily recognized overt suicidal behaviours. These reports recommended that a standardized method to ascertain the extent and nature of ISDBs be developed to examine the etiology and clinical correlates of these behaviours. This chapter reviews the existing literature on ISDBs and offers research, clinical, and policy recommendations.

INTRODUCTION:: One key activity of the University of South Florida Preparedness and Emergency Response Learning Center is designing, developing, and delivering community preparedness, response, and recovery system training. BACKGROUND/RATIONALE:: Coalitions are vital for addressing emergencies or disaster situations within communities. The University of South Florida Community-Based Disaster Coalition was designed to address the challenges of building and sustaining coalitions, emphasize methods to enhance their sustainability and effectiveness, and strengthen their purpose and community impact during disasters. METHODS/ACTIVITY:: Teams of participants were offered 2 years of training to support coalition-building efforts. In year 1, participants engaged in 3 days of facilitator-led instruction, hands-on activities, tabletop exercises, and breakout groups to learn techniques to strengthen their coalition, which are the focus of this study. In year 2, participants engaged in additional training through course refreshers, distance learning opportunities, and webinars. Participants were grouped by county or region and comprised 6 to 9 people from a range of backgrounds and professions. RESULTS/OUTCOMES:: During the 2012 (year 1) trainings, 184 people attended the program, representing nearly half (31; 46%) of Florida counties. Performance data indicated that participants significantly improved their knowledge scores, and course evaluations indicated that they were satisfied with the course overall. DISCUSSION:: The Community-Based Disaster Coalition trainings focused on community capacity of disaster response in 31 counties, which represents close to 13 million people or nearly three-fourths of Florida residents. Training evaluations supported previous findings regarding critical coalition elements for development and sustainment, such as clear coalition purpose and goals. LESSONS LEARNED/NEXT STEPS:: Several lessons were evident and inform future Community-Based Disaster Coalition efforts including adapting training to meet coalition needs; supporting the process of coalition building; following up with extended training opportunities and resources; continuing to provide trainings to counties that have not yet participated; and expanding training in other states, regions, territories, and internationally. Language: en

Previously, extracellular vesicle production in Gram-positive bacteria was dismissed due to the absence of an outer membrane, where Gram-negative vesicles originate, and the difficulty in envisioning how such a process could occur through the cell wall. However, recent work has shown that Gram-positive bacteria produce extracellular vesicles and that the vesicles are biologically active. In this study, we show that Bacillus subtilis produces extracellular vesicles similar in size and morphology to other bacteria, characterized vesicles using a variety of techniques, provide evidence that these vesicles are actively produced by cells, show differences in vesicle production between strains, and identified a mechanism for such differences based on vesicle disruption. We found that in wild strains of B. subtilis, surfactin disrupted vesicles while in laboratory strains harbouring a mutation in the gene sfp, vesicles accumulated in the culture supernatant. Surfactin not only lysed B. subtilis vesicles, but also vesicles from Bacillus anthracis, indicating a mechanism that crossed species boundaries. To our knowledge, this is the first time a gene and a mechanism has been identified in the active disruption of extracellular vesicles and subsequent release of vesicular cargo in Gram-positive bacteria. We also identify a new mechanism of action for surfactin.

Although numerous government, nonprofit, and relief organizations have endeavored to educate and prepare the American public for disasters, adults with physical, mental, and educational disabilities remain among the most vulnerable and least prepared subgroups of the population. The lack of alignment between the literacy demands of existing disaster preparedness and recovery materials and the literacy skills of many vulnerable subgroups limits their ability to understand and effectively use potentially life-saving information. We review the literature on literacy and vulnerable populations, propose a new model for disaster literacy, and describe opportunities for incorporating best practices into planning and preparedness activities. Disaster literacy is defined here as an individual's ability to read, understand, and use information to make informed decisions and follow instructions in the context of mitigating, preparing, responding, and recovering from a disaster. Recommendations are made for developing and evaluating disaster communication materials for vulnerable populations. To meet and improve the disaster literacy of vulnerable populations we suggest pilot-testing and evaluation be routinely used to inform selection of media type, message, and point of contact. (Disaster Med Public Health Preparedness. 2014;0:1–9)

Surges in demand for professional mental health services occasioned by disasters represent a major public health challenge. To build response capacity, numerous psychological first aid (PFA) training models for professional and lay audiences have been developed that, although often concurring on broad intervention aims, have not systematically addressed pedagogical elements necessary for optimal learning or teaching. We describe a competency-based model of PFA training developed under the auspices of the Centers for Disease Control and Prevention and the Association of Schools of Public Health. We explain the approach used for developing and refining the competency set and summarize the observable knowledge, skills, and attitudes underlying the 6 core competency domains. We discuss the strategies for model dissemination, validation, and adoption in professional and lay communities.

Graphical abstract, Many intracellular bacterial pathogens naturally release membrane vesicles (MVs) under a variety of growth environments. For pathogenic bacteria there are strong evidences that released MVs are a delivery mechanism for the release of immunologically active molecules that contribute to virulence. Identification of membrane vesicle-associated proteins that can act as immunological modulators is crucial for opening up new horizons for understanding the pathogenesis of certain bacteria and for developing novel vaccines. In this protocol, we provide all the details for isolating MVs secreted by either mycobacteria or Gram-positive bacteria and for the subsequent identification of the protein content of the MVs by mass spectrometry. The protocol is adapted from Gram-negative bacteria and involves four main steps: (1) isolation of MVs from the culture media; (2) purification of MVs by density gradient ultrucentrifugation; (3) acetone precipitation of the MVs protein content and in-solution trypsin digestion and (4) mass spectrometry analysis of the generated peptides and protein identification. Our modifications are:•Growing Mycobacteria in a chemically defined media to reduce the number of unrelated bacterial components in the supernatant.•The use of an ultrafiltration system, which allows concentrating larger volumes.•In solution digestion of proteins followed by peptides purification by ziptip.

Mental illness is one of the leading causes of disability, with direct and indirect costs posing a significant financial burden. Previously, a large prospective economic utility study (n>13,000) showed that the GeneSight® test, a psychiatric pharmacogenomic decision support tool powered by CPGx® technology, reduced medication costs, increased adherence, andreduced polypharmacy for patients who had failed monotherapy for psychiatric disorders. The current study, which is a sub-analysis of this larger study, assessed cost savings associated with combinatorial pharmacogenomic testing in patients with generalized anxiety disorder (GAD) and major depressive disorder (MDD). Medication costs were extracted using pharmacy claims data provided by Medco, a large pharmacy benefits manager, for patients with GAD (n=318) and MDD (n=459). Medication cost savings per member per year (PMPY) for 1 year following the test were compared between patients whose medication regimens were congruent with the test recommendations and those whose medication regimens were incongruent with these recommendations. When healthcare providers’ decisions were congruent with combinatorial pharmacogenomic testing, PMPY savings was $6,747 (pFunding AcknowledgementsResearch was funded by Assurex Health, Inc.

Background Controlled substance prescription use and abuse in the United States has become a public health epidemic. Specifically, benzodiazepine abuse in 2013 has increased as much 400 percent since 1996. Generalized Anxiety Disorder (GAD) was the most frequent reason these medications were given, accounting for 56% of prescriptions. Previously, GeneSight, a psychiatric pharmacogenomic decision support tool, was reported to significantly improve depression symptoms in treatment-resistant depression patients, reduce costs associated with prescriptions, and decrease polypharmacy. Methods To evaluate the combinatorial pharmacogenomics report as a tool to treat GAD and reduce the amount of benzodiazepines used, we analyzed data from two separate studies: (1) Individualized Medicine: Pharmacogenetic Assessment and Clinical Treatment (IMPACT) study, a longitudinal pilot research program , where 315 participants with anxiety disorders (N=210 with GAD) and follow-up data on symptom severity using the GAD-7 questionnaire were identified; and (2) MEDCO dataset (Winner et al., 2015; Curr Med Res Opin.;31:1633-43) in which patients (n=660) who were prescribed at least one benzodiazepine six months pre-testing and were followed six months post-testing. Results The analyses in the IMPACT study sample showed that medication treatment decisions congruent with the combinatorial pharmacogenomics GeneSight test results can help guide clinician treatment of GAD and result in significantly more improvement of anxiety symptom severity (congruent: -44.9±35.9% versus non-congruent: -25.5±31.8%; t=2.19; p=0.031). In the MEDCO dataset, we found that 18% (n=116) of the patients originally taking at least one benzodiazepine pre-testing (n=660) ceased their use of benzodiazepines post-testing with a significant decrease in benzodiazepine drug counts and refills after testing (mean count 1.2 vs. 0.9; p Discussion The GeneSight test can lead to more effective treatment of the anxiety symptoms in GAD patients and may lead to beneficial decrease in benzodiazepine use.

The Florida Public Health Training Center (FPHTC) at the University of South Florida (USF) College of Public Health (COPH) developed and implemented an Online Mentor Program (OMP) designed to suppo...

Possible factors in prejudice toward Muslims and those perceived to be Muslims were investigated. We specifically investigated cues of foreignness that may communicate threat. Using a 2 (Complexion: dark vs. light) × 2 (Dress: Middle Eastern vs. Western) × 2 (Name: Allen vs. Mohammed) between-subjects design, we expected cues of foreignness (dress and name) to have a greater impact on perceptions of targets than phenotype (complexion). Participants reviewed portraits of young men varying in the manipulated characteristics and gave their impressions. Generally, complexion did not affect perceptions, but portraits in Middle Eastern dress were rated less positively. There was a name by dress interaction in which Allen in Western dress was rated least negatively. Implications for future research are discussed.

With the recent broad acceptance of body worn cameras (BWC) for police departments, there is an increased need to perform video analytics for this domain. However, body worn cameras pose several challenges including severe motion blur, barrel camera distortion from wide angle lenses, close proximity and odd viewing angles, and poor lighting conditions. In this paper, we evaluate the performance of several state of the art face detection approaches including Aggregate Channel Features [1] and Faster R-CNN [2] and show their limitations in this domain. We then describe how face detection can be improved for BWC by corroborating information from body parts detection. We design a system using 0–1 linear integer programming to optimize the matching of body parts detections for each person in the scene and to maximize the hit rate of faces with supporting evidence. By leveraging information from body parts detection, we are able to improve the average precision by nearly two percent.

Lung cancer is the number one cause of cancer deaths. Many early stage lung cancer patients have a resectable tumor, however, their cardiopulmonary function needs to be properly evaluated before they are deemed operative candidates. Pulmonary function is assessed via spirometry and diffusion capacity. If these are below a certain threshold, cardiopulmonary exercise testing (CPET) is recommended. CPET is expensive, labor intensive, and sometimes ineffective since the patient is unable to fully participate due to comorbidities, such as limited mobility. In addition, CPET is done using a set of physical activities that may or may not be relevant to the patient’s typical activities. This paper presents steps towards developing a solution to address this gap. Specifically, we present OOCOO, a mobile mask system designed to measure oxygen and carbon dioxide levels in respiration, as well as activity levels. Unlike state of practice, oxygen, carbon dioxide, and activity data can be continuously measured over a long period of time in the patient’s environment of choice. The mask is capable of wireless data transfer to commodity smartphones. We have carried out initial work on development of an Android application to capture, analyze, and share the data with authorized entities.

The genetic structure of human populations is often characterized by aggregating measures of ancestry across the autosomal chromosomes. While it may be reasonable to assume that population structure patterns are similar genome-wide in relatively homogeneous populations, this assumption may not be appropriate for admixed populations, such as Hispanics and African-Americans, with recent ancestry from two or more continents. Recent studies have suggested that systematic ancestry differences can arise at genomic locations in admixed populations as a result of selection and nonrandom mating. Here, we propose a method, which we refer to as the chromosomal ancestry differences (CAnD) test, for detecting heterogeneity in population structure across the genome. CAnD can incorporate either local or chromosome-wide ancestry inferred from SNP genotype data to identify chromosomes harboring genomic regions with ancestry contributions that are significantly different than expected. In simulation studies with real genotype data from phase III of the HapMap Project, we demonstrate the validity and power of CAnD. We apply CAnD to the HapMap Mexican-American (MXL) and African-American (ASW) population samples; in this analysis the software RFMix is used to infer local ancestry at genomic regions, assuming admixing from Europeans, West Africans, and Native Americans. The CAnD test provides strong evidence of heterogeneity in population structure across the genome in the MXL sample (p=1e−5), which is largely driven by elevated Native American ancestry and deficit of European ancestry on the X chromosomes. Among the ASW, all chromosomes are largely African derived and no heterogeneity in population structure is detected in this sample.

Gordonia sihwensis strain 9 is a Gram-positive bacterium capable of efficient aerobic degradation of branched and normal alkanes. The draft genome of G. sihwensis S9 is 4.16 Mb in size, with 3,686 coding sequences and 68.1% G+C content. Alkane monooxygenase and P-450 cytochrome genes required for alkane degradation are predicted in G. sihwensis S9.

Faster R-CNN achieves state-of-the-art performance on generic object detection. However, a simple application of this method to a large vehicle dataset performs unimpressively. In this paper, we take a closer look at this approach as it applies to vehicle detection. We conduct a wide range of experiments and provide a comprehensive analysis of the underlying structure of this model. We show that through suitable parameter tuning and algorithmic modification, we can significantly improve the performance of Faster R-CNN on vehicle detection and achieve competitive results on the KITTI vehicle dataset. We believe our studies are instructive for other researchers investigating the application of Faster R-CNN to their problems and datasets.

Platelets play an essential role in hemostasis and thrombosis. We performed a genome-wide association study of platelet count in 12,491 participants of the Hispanic Community Health Study/Study of Latinos by using a mixed-model method that accounts for admixture and family relationships. We discovered and replicated associations with five genes (ACTN1, ETV7, GABBR1-MOG, MEF2C, and ZBTB9-BAK1). Our strongest association was with Amerindian-specific variant rs117672662 (p value = 1.16× 10(-28)) in ACTN1, agene implicated in congenital macrothrombocytopenia. rs117672662 exhibited allelic differences in transcriptional activity and protein binding in hematopoietic cells. Our results underscore the value of diverse populations to extend insights into the allelic architecture of complex traits.