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Reaction of cyclometallated iridium(III) complex Ir(ppy)2(PPh3)Cl (2, ppy = 2-phenylpyridine) with dicyanamide or tricyanomethanide gave neutral mononuclear complexes Ir(ppy)2(PPh3)N(CN)2 (3a) or Ir(ppy)2(PPh3)C(CN)3 (3b), and dicyanamide/tricyanomethanide-linked binuclear iridium(III) complexes [{Ir(ppy)2(PPh3)}2N(CN)2]+ (4a) or [{Ir(ppy)2(PPh3)}2C(CN)3]+ (4b). Substitution of coordinated chloride in the precursor 2 with dicyanamide or tricyanomethanide improved significantly the luminescence properties of 3a–4b. Compared with that in the precursor 2 (1.6%), 2.2 to 9.3-fold enhancement of emission quantum yields was detected in 3a–4b.

The preparation, characterization and photophysical properties of heterobinuclear complexes {Pt(C^N^N)(C Cbpy)}Ln(hfac)3 (C^N^N = 2-(6-(naphthalen-3-yl)-4-phenylpyridin-2-yl)pyridine; HC Cbpy = 5-ethynyl-2,2′-bipyridine; Ln = Nd, Eu, Yb; hfac = hexafluoroacetylacetonate) are described. With excitation at 390 ⩽ λex ⩽ 500 nm which is the MLCT/LLCT absorption region of the Pt(C^N^N)(C Cbpy) chromophore, lanthanide luminescence is successfully attained by Pt → Ln energy transfer from the platinum(II) antenna chromophore to the lanthanide center across the bridging C Cbpy ligand.

The structures of a one-dimensional compound Cu(dien)V2O6·H2O 1 and a two-dimensional compound Ni(dien)V2O6 2, where dien is diethylenetriamine, have been determined by X-ray diffraction. The former is composed of corner-sharing VO4 tetrahedra chains, with Cu(dien)2+ fragments attached to every other VO4 unit. The latter has a layer structure with the V4O12 tetramers connected by edge-shared Ni2(µ-O)2(dien)24+ dimers. The layers are further stacked to form one-dimensional channels along the [001] direction. Magnetic susceptibility measurements for both compounds have been performed down to 2 K. Paramagnetic behavior is observed in 1, whereas ferromagnetic interaction is found to be dominant in 2.

A new pillared layered mixed-valence copper phosphonate Na2Cu15(hedp)6(OH)2(H2O), where hedp represents 1-hydroxyethylidenediphosphonate anion, has been hydrothermally synthesized and structurally determined. Crystal data: trigonal, space group P, a = b = 11.720(2), c = 9.531(1) A, U = 1134(2) A3, Z = 1. The structure consists of honeycomb layers of edge-sharing (CuII3O)O3 units (sheet A) and edge-sharing NaO6 octahedra and CuIIO4 planes (sheet B). The layers are pillared alternately through 18-membered rings which are made up from the organic backbone of the diphosphonate groups and the CuIO2 units. A three-dimensional framework is thus formed which contains intersecting channels where the H2O molecules reside. The magnetic properties of the compound have been investigated. No long-range ordering has been found down to 2 K.

Crystallographic characterization of the metastable alkynyl complexes [Au6Ag13(μ-dppm)3(μ3-η1-C⋮CC6H4R-4)14]5+ (R = H (1a), CH3 (2a), But (3a)) is described. Photolysis of the green Au6Ag13 alkynyl...

A series of highly phosphorescent PtAg2 heterotrinuclear alkynyl complexes with bis(diphenylphosphinomethyl)phenylphosphine (dpmp) were prepared and characterized structurally. The solution phosphorescence with various emitting colors is systematically modulated by modifying substituents as well as π-conjugated systems in aromatic acetylides. The crystals, powders, or films exhibit reversible stimuli-responsive phosphorescence changes upon exposure to vapor of MeCN, pyridine, DMF, etc., resulting from perturbation of d(8)-d(10) metallophilic interaction in the excited states as a consequence of the formation/disruption of Ag-solvent bonds. Both experimental and time-dependent density functional theory (TD-DFT) studies demonstrate that d(8)-d(10) metallophilic interaction exerts a crucial role on phosphorescent characteristics due to the PtAg2 cluster-based (3)[d → p] state. This study affords a paradigm for phosphorescence modulation in d(8)-d(10) heteronuclear complexes.

Grain weight, an essential yield component, is under strong genetic control and markedly influenced by the environment. Here, by genome-wide association analysis with a panel of 94 elite common wheat varieties, 37 loci were found significantly associated with thousand-grain weight (TGW) in one or more environments differing in water and fertiliser levels. Five loci were stably associated with TGW under all 12 environments examined. Their elite alleles had positive effects on TGW. Four, two, three, and two loci were consistently associated with TGW in the irrigated and fertilised (IF), rainfed (RF), reduced nitrogen (RN), and reduced phosphorus (RP) environments. The elite alleles of the IF-specific loci enhanced TGW under well-resourced conditions, whereas those of the RF-, RN-, or RP-specific loci conferred tolerance to the TGW decrease when irrigation, nitrogen, or phosphorus were reduced. Moreover, the elite alleles of the environment-independent and -specific loci often acted additively to enhance TGW. Four additional loci were found associated with TGW in specific locations, one of which was shown to contribute to the TGW difference between two experimental sites. Further analysis of 14 associated loci revealed that nine affected both grain length and width, whereas the remaining loci influenced either grain length or width, indicating that these loci control grain weight by regulating kernel size. Finally, the elite allele of Xpsp3152 frequently co-segregated with the larger grain haplotype of TaGW2-6A, suggesting probable genetic and functional linkages between Xpsp3152 and GW2 that are important for grain weight control in cereal plants. Our study provides new knowledge on TGW control in elite common wheat lines, which may aid the improvement of wheat grain weight trait in further research.

Octahedral hexanuclear ferrocenylacetylide complexes [Ag6(μ-dppm)2(μ3-η1-CCFc)4(CH3OH)2](BF4)2 (1) and [Cu6(μ-dppm)2(μ3-η1-CCFc)4(ClO4)2] (2) (dppm=Ph2PCH2PPh2, Fc=ferrocenyl) are synthesised and characterized structurally.

Nasopharyngeal carcinoma (NPC) is a type of head and neck cancer with significantly high prevalence in Southern China. Unlike other head and neck cancers, mutations or deletions of tumor suppressor genes in NPC are not common. Recently, downregulation of tumor suppressor genes expression by microRNA (miRNA) is increasingly recognized as an important mechanism of nasopharyngeal tumorigenesis. In this study, we reported that microRNA-144 (miR-144) was frequently upregulated in NPC specimens and cell lines. Repression of miR-144 significantly decreased cell proliferation, clonogenicity, migration, invasion and tumor formation in nude mice, while restoring miR-144 in miR-144-attenuated NPC cells exhibited a strong tumorigenic role. Further, we found that miR-144 was inversely correlated with the tumor suppressor gene phosphatase and tensin homolog (PTEN) in NPC specimens and cell lines, and then we identified PTEN as a direct target of miR-144 in NPC cell lines. PTEN downregulation in miR-144-attenuated cells could increase cell growth, migration and invasion. Mechanistic investigations revealed that miR-144 suppressed the expression of PTEN to increase the expression of pAkt and cyclin D1 to promote G(1)-phase transition and decrease E-cadherin to promote migration and invasion. Taken together, we provide compelling evidence that miR-144 functions as an onco-miRNA in NPC, and its oncoeffects are mediated chiefly by repressing PTEN expression to activate the PI3K/Akt pathway.

The reaction between [Cu2(Ph2PNHPPh2)2(MeCN)2](BF4)2 and sodium salt of mercaptoacetic acid (HSCH2COONa) at room temperature for 12 h produced a doubly μ3-SH-bridged tricopper complex [Cu3(μ-Ph2PNHPPh2)3(μ3-SH)2](BF4)·2MeOH·Et2O which affords a trigonal bipyramidal core of Cu3(μ3-SH)2 with copper ⋯ copper separations around 3.0 A. The complex is photoluminescent and it displays an emissive maximum at 523 nm in the solid state at 293 K.

In protein coding regions prediction works, the predicted non-coding percentile (PNCP) is usually used as the threshold to separate the DNA sequences into two kinds of regions, i.e. protein coding regions and non-coding regions. In this paper, a new protein coding regions prediction algorithm based on the hybrid threshold is presented. First, the normalized power spectral density (PSD) of a DNA sequence is calculated using the prediction algorithm based on narrow pass-band filters (NPBF), and the maximum PSD value of the DNA sequence is used as the normalization standard in the NPBF algorithm. Second, the coding regions' PSD curve is set up as a trapezoid model characterized by its slope and height. Third, the method for calculating the hybrid threshold which taking the slope and the height both into consideration is presented. Finally, the algorithm is performed on DNA dataset HMR195. Using the approximate correlation (AC) as the evaluation measure of prediction accuracy, the prediction results of the proposed algorithm reach to 0.48 for the dataset, which is much better than the modified Gabor wavelet transform algorithm. The prediction results are also presented in the form of q9 proposed by Chun-Ting Zhang in 2002.

Deletion of the short arm of chromosome 3 is one of the most frequent genetic alterations in many solid tumors including nasopharyngeal carcinoma (NPC), suggesting the existence of one or more tumor suppressor genes (TSGs) within the frequently deleted region. A putative TSG RBMS3 (RNA binding motif, single stranded interacting protein 3), located at 3p24-p23, has been identified in our previous study. Here, we reported that downregulation of RBMS3 was detected in 3/3 NPC cell lines and 13/15 (86.7%) primary NPC tissues. Functional studies using both overexpression and suppression systems demonstrated that RBMS3 has a strong tumor suppressive role in NPC. The tumor suppressive mechanism of RBMS3 was associated with its role in cell cycle arrest at the G1/S checkpoint by upregulating p53 and p21, downregulating cyclin E and CDK2, and the subsequent inhibition of Rb-ser780. Further analysis demonstrated that RBMS3 had a pro-apoptotic role in a mitochondrial-dependent manner via activation of caspase-9 and PARP. Finally, RBMS3 inhibited microvessel formation, which may be mediated by down-regulation of MMP2 and β-catenin and inactivation of its downstream targets, including cyclin-D1, c-Myc, MMP7, and MMP9. Taken together, our findings define a function for RBMS3 as an important tumor suppressor gene in NPC., published_or_final_version

An optimized fast fixed point algorithm based on modified Newton iteration method has been proposed. With good performance ofthe blind image separation, the optimized algorithm can improve the convergence speed greatly.We proposed a new adaptive enhancement parameter to enhance the separated images effectively. The experimental results demonstrate that the new algorithm is superior.

Planar platinum(II) complexes Pt(bpyC≡CSiMe(3))(C≡CC(6)H(4)R-4)(2) (R = H (1), Bu(t) (2)) with 5-trimethylsilylethynyl-22'-bipyridine show an unusual, reversible, and reproducible mechanical stimuli-responsive color and luminescence switch. When crystalline 1 or 2 is ground, bright yellow-green emitting is immediately converted to red luminescence with an emission red shift of 121-155 nm for 1 or 53-89 nm for 2. Meanwhile, the crystalline state is transformed to an amorphous phase that can be reverted to the original crystalline state by organic vapor adsorbing or heating, along with red luminescence turning back to yellow-green emitting. The reversibility and reproducibility of luminescence mechanochromic properties have been dynamically monitored by the variations in emission spectra and X-ray diffraction patterns. The drastic grinding-triggered emission red shift is likely involved in the formation of a dimer or an aggregate through Pt-Pt interaction, resulting in a conversion of the (3)MLCT/(3)LLCT emissive state in the crystalline state into the (3)MMLCT triplet state in the amorphous phase. Compared with the drastic grinding-triggered emission red shift in 1 (121-155 nm), the corresponding response shift in 2 (53-89 nm) is much smaller since a bulky tert-butyl in C≡CC(6)H(4)bu(t)-4 induces the planar platinum(II) molecules to stack through a longer Pt-Pt distance and less intermetallic contact compared with that in 1, as suggested from EXAFS studies.

Objectives Interaction between neoplastic and stromal cells plays an important role in tumour progression. It was recently found that WNT2 was frequently overexpressed in fibroblasts isolated from tumour tissue tumour fibroblasts (TF) compared with fibroblasts from non-tumour tissue normal fibroblasts in oesophageal squamous cell carcinoma (OSCC). This study aimed to investigate the effect of TF-secreted Wnt2 in OSCC development via the tumourestroma interaction. Methods Quantitative PCR, western blotting, immunohistochemistry and immunofluorescence were used to study the expression pattern of Wnt2 and its effect on the Wnt/b-catenin pathway. A Wnt2-secreting system was established in Chinese hamster ovary cells and its conditioned medium was used to study the role of Wnt2 in cell proliferation and invasion. Results Expression of Wnt2 could only be detected in TF but not in OSCC cancer cell lines. In OSCC tissues, Wnt2 (+) cells were mainly detected in the boundary between stroma and tumour tissue or scattered within tumour tissue. In this study, Wnt2-positive OSCC was defined when five or more Wnt2(+) cells were observed in 2003 microscopy field. Interestingly, Wnt2-positive OSCC (22/51 cases) was significantly associated with lymph node metastases (p¼0.001), advanced TNM stage (p¼0.001) and disease-specific survival (p

A series of platinum(II) complexes with 1,3-bis(2-pyridylimino)isoindoline (BPI) derivatives were prepared by substitution of the coordinated Cl in the precursor complex Pt(BPI)Cl with a N-heterocyclic ligand such as pyridine, phthalazine or phenanthridine. These complexes display orange to red luminescence in fluid dichloromethane solutions and in the solid states at room temperature. The photophysical properties were tuned by introducing electron-withdrawing -NO(2) or electron-donating -NH(2) to the BPI ligand. The DFT computational studies suggest that the emission in the N-heterocyclic ligand substituted platinum(II) complexes originates mainly from the (3)[π→π*(BPI)] (3)IL triplet excited state, mixed with some (3)[dπ(Pt)→π*(BPI)] (3)MLCT character. Compared with the precursor Pt(BPI)Cl, both the low-energy absorption and the emission in the N-heterocyclic ligand substituted platinum(II) complexes exhibits a distinct blue-shift due to an obviously enhanced contribution from the (3)IL state and a reduced (3)MLCT character.

According to the principle of the weak economy, mathematical model of emergency logistics routing optimization has been established in this paper, and the model is solved by the uniform mutation ant colony algorithm, simulation shows that the optimal results of uniform mutation ant colony algorithm are better than ant colony algorithm. At the same time, compared with the mathematical model of the smallest time, mathematical model of emergency logistics routing optimization reduce the distribution costs on the basis of time requirements which should be satisfied.

Background In order to help establish heterotic groups of Chinese northern wheat cultivars (lines), Diversity arrays technology (DArT) markers were used to investigate the genetic diversity and population structure of Chinese common wheat (Triticum aestivum L.). Results In total, 1637 of 7000 DArT markers were polymorphic and scored with high confidence among a collection of 111 lines composed mostly of cultivars and breeding lines from northern China. The polymorphism information content (PIC) of DArT markers ranged from 0.03 to 0.50, with an average of 0.40, with P > 80 (reliable markers). With principal-coordinates analysis (PCoA) of DArT data either from the whole genome or from the B-genome alone, all lines fell into one of two major groups reflecting 1RS/1BL type (1RS/1BL and non-1RS/1BL). Evidence of geographic clustering of genotypes was also observed using DArT markers from the A genome. Cluster analysis based on the unweighted pair-group method with algorithmic mean suggested the existence of two subgroups within the non-1RS/1BL group and four subgroups within the 1RS/1BL group. Furthermore, analysis of molecular variance (AMOVA) revealed highly significant (P < 0.001) genetic variance within and among subgroups and among groups. Conclusion These results provide valuable information for selecting crossing parents and establishing heterotic groups in the Chinese wheat-breeding program.

Cadmium sulfide (CdS) nanoparticles of about 3 nm in diameter have been prepared by the sonochemical reduction of a mixed solution of cadmium chloride (CdCl2), sodium thiosulfate (Na2S2O3) and isopropyl alcohol ((CH3)2CHOH) in Ar atmosphere at room temperature. The CdS nanoparticles were characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). The formation mechanism of CdS nanoparticles is also discussed together with the optical absorption spectroscopy analysis. The method presented here can be used for the preparation of other sulfide nanoparticles.

A major objective of quantitative trait locus (QTL) studies is to find genes/markers that can be used in breeding programs via marker assisted selection (MAS). We surveyed the QTLs for yield and yield-related traits and their genomic distributions in common wheat (Triticum aestivum L.) in the available published reports. We then carried out a meta-QTL (MQTL) analysis to identify the major and consistent QTLs for these traits. In total, 55 MQTLs were identified, of which 12 significant MQTLs were located on wheat chromosomes 1A, 1B, 2A, 2D, 3B, 4A, 4B, 4D and 5A. Our study showed that the genetic control of yield and its components in common wheat involved the important genes such as Rht and Vrn. Furthermore, several significant MQTLs were found in the chromosomal regions corresponding to several rice genomic locations containing important QTLs for yield related traits. Our results demonstrate that meta-QTL analysis is a powerful tool for confirming the major and stable QTLs and refining their chromosomal positions in common wheat, which may be useful for improving the MAS efficiency of yield related traits.

In RFID system, more than one tag answering the query of reader together can result in tag collision, which makes the communication between reader and tag failure. For the problem, a novel anti-collision algorithm was present in the paper. The new algorithm transmitted the maximal collision bit position but the serial number of tag, which reduced the data transmitted greatly, and uses splitting to make the algorithm working in a smaller scope which reduces the number of searching. Computer simulation result shown that the new algorithm was efficient.

In RFID systems, some readers will suffer from RF interference, which can lead to reader collision. Consequently, an anti-collision algorithm based on distributed power control (DPC) scheme was proposed as a solution for the problem in this paper. The proposed algorithm dynamically adjusts power output of a RFID reader based on the signal-to-interference ratio (SIR) of RFID reader receiver, minimizes the reader collision ratio while maintaining a desired read range. The simulation results shown that the proposed algorithm reduces the collision ratio greatly while ensure overall coverage area of the RFID system.

Multifunctional heteropentanuclear Al(3)Ln(2) (Ln = Nd, Eu, Yb) clusters with a novel structure exhibit significantly fluoride-enhanced lanthanide emission intensity and lifetime in both solid state and solution as well as unusual white-light emitting for an Al(3)Eu(2) complex.

The reaction of 1,2-bis(5-(diphenylphosphino)-2-methylthien-3-yl) cyclopentene (PPh(2)-DTE-PPh(2)) with the triruthenium cluster precursor [Ru(3)O(OAc)(6)(py)(2)(CH(3)OH)](PF(6)) (1) gave monomeric or dimeric derivatives [Ru(3)O(OAc)(6)(py)(2){PPh(2)-DTE-PPh(2)}](PF(6)) ([2]PF(6)) and [{Ru(3)O(OAc)(6)(py)(2)}(2){mu-PPh(2)-DTE-PPh(2)}](PF(6))(2) ([3]PF(6)). Reduction of [2](+) and [3](2+) afforded one- or two-electron-reduced neutral products Ru(3)O(OAc)(6)(py)(2){PPh(2)-DTE-PPh(2)} (2) and {Ru(3)O(OAc)(6)(py)(2)}(2){mu-PPh(2)-DTE-PPh(2)} ()3, respectively. These triruthenium complexes show remarkable photochromism through photochemical ring-closing (UV light irradiation) and ring-opening (Vis light irradiation) processes as well as electrochromic properties through oxidation/reduction in the triruthenium cluster. Both the photochromic and electrochromic properties of 2 and 3 are highly reversible.

Platinum(II) complex [Pt(Me(3)SiC[triple bond]CbpyC[triple bond]CSiMe(3))(C[triple bond]CPh)(2)] (1) with 5,5-bis(trimethylsilylethynyl)-2,2'-bipyridine (Me(3)SiC[triple bond]CbpyC[triple bond]CSiMe(3)) and phenylacetylene (PhC[triple bond]CH) exhibits unusual luminescence vapochromism to volatile organic compounds (VOCs) including CH(2)Cl(2), CHCl(3), and CH(3)I, which is useful for detection of volatile halohydrocarbon with one carbon atom and molecular weight less of than 150. Crystal structural determination of 1, 1 x CHCl(3), 1 x 1/2(CH(2)ClCH(2)Cl), and 1 x 1/2(toluene) revealed a staggered packing for 1 x CHCl(3) whereas there was an antiparallel packing for other three structures in stacking of planar platinum(II) moieties. The Pt...Pt distance is 3.302(1) A in 1 x CHCl(3), whereas it is4.0 A in the other three structures. Complex 1 displays bright orange luminescence in dichloromethane solution, arising from pi(phenylacetylide)--pi*(Me(3)SiC[triple bond]CbpyC[triple bond]CSiMe(3)) (3)LLCT and d(Pt)--pi*(Me(3)SiC[triple bond]CbpyC[triple bond]CSiMe(3)) (3)MLCT triplet states which are supported by DFT calculation. The solid-state emission occurs at approximately 762 nm for 1 x VOC (VOC = CH(2)Cl(2), CHCl(3), and CH(3)I), whereas it was at approximately 562 (603sh) or 603 (562sh) nm for 1 and other 1 x VOC, corresponding to a vapochromic response shift of approximately 160-200 nm. The dramatic vapochromism and vapoluminescence of 1 to the vapor of CH(2)Cl(2), CHCl(3), or CH(3)I are induced by a reversible conversion of the emissive state from (3)MLCT/(3)LLCT character to (3)MMLCT/(3)LLCT state.

Background Many studies have shown that colon cancer is an estrogen-dependent carcinoma. This study explored the efficacy of endocrine therapy in colon cancer cells with high metastatic potential (HT29). We investigated the proliferation of HT29 cells after exposure to endocrine therapy (tamoxifen) and 5-FU. Methods Apoptosis was evaluated using flow cytometry. The expression of matrix metalloproteinases 7 (MMP-7) and estrogen receptor beta (ERβ) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and western blot. The migration capability of treated cells was determined with wound scratch assay. Results Tamoxifen alone, 5-FU alone, and the combination of the two drugs can significantly inhibit HT29 cell proliferation and migration, block the cells in G2/M phase and induce cell apoptosis. These drugs also can down-regulate MMP7 and ERβ expression. Conclusion Our findings suggest that endocrine therapy is an efficient therapy for inhibiting ERβ-positive colon cancer cell proliferation and migration via down-regulation of MMP7.

The therapeutic effects of chemotherapy on gastric cancer are still unsatisfactory. Predicting chemosensitivity of tumors as therapeutic guidance could help to improve the efficacy of chemotherapy. This study was to evaluate the chemosensitivity of gastric cancer to single or combined therapeutic agents by histoculture drug response assay, to evaluate the expression levels of multidrug resistance (MDR) genes and proteins and analyze their correlations to the chemosensitivity of gastric cancer.The inhibitory effects of single agents, including epirubicin, cisplatin (DDP), oxaliplatin, 5-fluorouracil (5-FU), taxetere, irinotecan, and combined regimens, including 5-FU, epirubicin plus DDP, 5-FU plus irinotecan, 5-FU plus oxaliplatin, and 5-FU, taxetere plus DDP, on 22 specimens of gastric cancer were evaluated by histoculture drug response assay. The mRNA and protein expression of MDR1, MRP1 and ABCG2 in gastric cancer were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.The inhibition rate of gastric cancer by 5-FU (29.8%) and sensitivity of gastric cancer to 5-FU (50.0%) were the highest among single agents; the inhibition rate of gastric cancer by 5-FU, taxetere plus DDP (59.8%) and sensitivity to 5-FU, epirubicin plus DDP (77.3%) were the highest among combined regimens; combined regimens achieved higher inhibition rate and sensitivity as compared with single agents (P0.05). The positive rates of MDR1, MRP1, and ABCG2 mRNA were 90.9%, 54.5%, and 77.3%; the positive rates of MDR1, MRP1, and ABCG2 proteins were 36.4%, 54.5%, and 36.4%. High expression of MDR proteins in gastric cancer was related with the resistance to epirubicin (P0.05).High expression of MDR1, MRP1, ABCG2 proteins in gastric cancer is related with the resistance to epirubicin, which indicates that these MDR genes may play a role in conferring the drug resistance to epirubicin.

A platinum(II) complex of 5,5'-bis(trimethylsilylethynyl)-2,2'-bipyridine displays unusually reversible colour and luminescence changes when exposed to specific organic vapours, correlated well with Pt...Pt distances as well as pi-pi and C-H...(C[triple bond]C) interactions in the stacking of square-planar platinum(II) moieties.

This study was purposed to investigate the expression of annexin II in patients with hematologic malignancies and its role in genesis and development of hematologic malignancies. The expression levels of annexin II in bone marrow cells from untreated 81 patients with acute leukemia, 6 patients with MM and 20 patients with iron deficiency anemia were detected by real-time PCR assay. The results showed that the expression of annexin II mRNA significantly increased in M(3) patients as compared with others, the expression of annexin II gene in groups M(5), MM, M(4) were higher than that of other groups except M(3) group, and there were no significant difference in expression of annexin II gene between M(1) + M(2) groups and controls. It is concluded that the expression of annexin II gene significantly increased in patients M(5), M(4), MM, who showed higher ratio of infiltration than other patients. It is inferred that the annexin II participates in invasion and infiltration of hematologic malignancies probably through enhancing the degradation of extracellular matrix by cells of hematologic malignancies.

The Human papillomavirus type 18L1 (HPV18L1) gene was synthesized by overlapping PCR after optimization using plant preferred codons.The gene sequences of HPV18L1 were obtained from GenBank and analyzed using DNAMAN, Lasergene, Vector NTI and BLAST. The target sequence was selected and modified using plant preferred codons by the Synthetic Gene Designer and JCat (Java Codon Adaptation Tool) with the addition of a His-tag to the C-terminus to construct the full-length modified HPV18L1 (mHPV18L1). mHPV18L1 was divided into 5 large segments, namely LS1 to LS5, with sizes ranging from 204 to 477 bp. Forty-three small oligonucleotide fragments with sizes of 57-59 bp and 6 pairs of primers were designed and synthesized. mHPV18L1 was amplified by overlapping PCR and subcloned into pMD18-T vector. The recombinant plasmid was identified by restriction enzymes digestion and sequencing.mHPV18L1 was successfully assembled using overlapping PCR. The results of digestion with restriction enzymes and PCR amplification confirmed that the recombinant vector pMD18T- mHPV18L1 contained the inserts with expected size of 1749 bp. mHPV18L1 sequence was confirmed by sequencing.mHPV18L1 with plant preferred codons and the recombinant vector pMD18T- mHPV18L1 have been obtained.

Diversity Array Technology (DArT) markers were used to investigate the genetic diversity, population structure, and extent of linkage disequilibrium (LD) on a genome-wide level in Canadian barley (Hordeum vulgare L.). Approximately 1,000 DArT markers were polymorphic and scored with high confidence among a collection of 170 barley lines composed mostly of Canadian cultivars and breeding lines. The reproducibility of DArT markers proved very high, as 99.9% of allele calls were identical among seven replicated samples. The polymorphism information content (PIC) of DArT markers ranged between 0.04 and 0.50 with an average of 0.38. Using principal coordinate analysis (PCoA), most lines fell into one of two major groups reflecting inflorescence type (two-row versus six-row). Within these two large groups, evidence of geographic clustering of genotypes was also observed. A cluster analysis Unweighted Pair Group Method with Algorithmic Mean suggested the existence of three subgroups within the two-row group and four subgroups within the six-row group. An analysis of molecular variance (AMOVA) revealed highly significant (P0.001) genetic variance within subgroups, among subgroups, and among groups. Values of LD, expressed as r (2), declined with increasing genetic distance, and mean values of r (2) fell below 0.2 for markers located 2.6 cM apart. Approximately 8% of marker pairs located on the same chromosome and 3.4% of pairs located on different chromosomes were in LD (r ( 2 )0.2). Within both the subsets of two-row and six-row lines, LD extended slightly further (3.5 cM) than for the entire set, while 7.5% of intra-chromosomal locus pairs and2% of inter-chromosomal pairs were in LD. We discuss the implications of these findings with regard to the prospects of association mapping of complex traits in barley.

Studies have shown that estrogen receptor beta (ERbeta), which is highly expressed in colorectal cancer, is related to tumor metastasis. Matrix metalloproteinase (MMP)-7 is overexpressed in colorectal cancer, and plays an important role in tumor invasion and metastasis. This study was to explore the effect of tamoxifen (TAM) on apoptosis and MMP-7 expression in ERbeta-positive human colorectal cancer cell line HT-29.After exposure to TAM, the proliferation of HT-29 cells was detected by MTT assay; cell apoptosis was evaluated using flow cytometry; expressions of ERbeta and MMP-7 were measured by Western blot.TAM significantly inhibited cell growth of HT-29 in a time (24 h, 48 h, 72 h )-and dose (0, 10(-7), 10(-6), 10(-5),0 10(-4) mol/L )-dependent manner. TAM exposure caused significant cell apoptosis of HT-29 cells at the concentration of 10(-4) mol/L [(69.9+/-4.2)%]. Moreover, TAM could bind to ERbeta to down-regulate MMP-7 protein expression in HT-29 cells.High concentration of TAM can inhibit the proliferation of ERbeta-positive HT-29 cells, and effectively bind with ERbeta to down-regulate MMP-7 expression.

A series of mono-, di- and trimeric oxo-centred triruthenium cluster compounds with ortho-metallated polypyridyl ligands were prepared by reaction of oxo-centred triruthenium precursor compound [Ru3O(OAc)6(py)2(CH3OH)]+ (1) with bis(2,2'-bipyridin-5-yl)butadiyne (bpyC[triple bond, length as m-dash]C-C[triple bond, length as m-dash]Cbpy), bis(2,2'-bipridin-5-yl)ethyne (bpyC[triple bond, length as m-dash]Cbpy), 2,2'-bipyrazine (bpz), pyrazino[2,3-f]quinoxaline (pyq), or 4,7-phenanthroline (4,7-phen). As revealed by redox wave splitting, electronic interactions are operating between two Ru3O redox centres in most of the triruthenium cluster dimers and trimers. The cluster-cluster interactions are highly sensitive to the variations in bonding modes of the polypyridyl ligands. Ortho-metallation of the bridging ligand induces an enhanced electronic interaction between two triruthenium centres.

Reaction of Pt(dppm-P,P')Cl2 (dppm = 1,2-bis(diphenylphosphino)methane) with HCCPhtpy (HCCPhtpy = 4'-(4-ethynylphenyl)-2,2':6',2"-terpyridine) in the presence of copper(I) iodide and diisopropylamine induced isolation of mononuclear complex cis-Pt(dppm-P,P')(C[triple bond]CPhtpy)2 (1), which can be converted into face-to-face diplatinum(II) species Pt2(mu-dppm)2(C[triple bond]CPhtpy)4 (5) when equivalent dppm is added. Incorporating 1 or 5 to Ln(hfac)3(H2O)2 (Hhfac = hexafluoroacetylacetone) gave PtLn2 (Ln = Nd (2), Eu (3), Yb (4)) or Pt2Ln4 (Ln = Nd (6), Eu (7), Gd (8), Yb (9)) adducts with the lanthanide centers chelated by terdentate terpyridyl in the bridging C[triple bond]CPhtpy. The structures of 1, 6, 7, and 9 were determined by X-ray crystallography. Upon excitation at lambdaex = 360-450 nm (2-4) or 360-500 nm (6-9), where the PtII alkynyl antenna chromophores absorb strongly but the model complexes Ln(hfac)3(HC[triple bond]CPhtpy) lack obvious absorption in this region, these PtLn2 and Pt2Ln4 (Ln = Nd, Eu, Yb) species exhibit band-like lanthanide luminescence that is typical of the corresponding Ln3+ ions, demonstrating unambiguously that efficient Pt --> Ln energy transfer occurs indeed from the PtII alkynyl antenna chromophores to the lanthanide centers across the bridging CCPhtpy with intramolecular Pt...Ln distances being ca. 14.2 A. The Pt --> Ln energy transfer rate (kET) is 6.07 x 10(7) s(-1) for Pt2Nd4 (6) and 2.12 x 10(5) s(-1) for Pt2Yb4 (9) species.

A series of platinum(II) terpyridyl complexes with phenolic ethynyl ligands have been synthesized and characterized. Their photophysical and sensing properties towards anions such as fluoride, acetate and dihydrogenphosphate have been investigated. These complexes show a colorimetric response and fluorescence quenching in the presence of anions including fluoride, acetate and dihydrogenphosphate, and selective sensing towards fluoride in some cases. The sensing mechanism has been investigated by spectrophotometric and 1H NMR titration.

Heterododecanuclear Pt(6)Ln(6) (Ln = Nd, Yb) complexes of 4-ethynyl-2,2'-bipyridine (HC[triple bond, length as m-dash]Cbpy), prepared using emissive Pt(Me(3)SiC[triple bond, length as m-dash]Cbpy)(C[triple bond, length as m-dash]Cbpy)(2) as an alkynyl bridging "ligand", afford sensitized near-infrared (NIR) lanthanide luminescence by Pt --Ln energy transfer from both Pt(bpy)(acetylide)(2) and Pt(2)(dppm)(2)(acetylide)(2) chromophores.

Reaction of oxo-centered Ru(3)(III,III,III) precursor [Ru(3)O(OAc)(6)(py)(2)(CH(3)OH)](PF(6)) (1) with 1 equiv of 2,2'-azobispyridine (abpy) or 2,2'-azobis(5-chloropyrimidine) (abcp) induced the formation of stable Ru(3)(III,III,II) derivatives [Ru(3)O(OAc)(5){mu-eta(1)(N),eta(2)(N,N)-L}(py)(2)](PF(6)) (L = abpy (2), abcp (3)). As established in the structure of 3 by X-ray crystallography, 2 or 3 is derived from 1 by substitution of the axial methanol and one of the bridging acetates in the parent Ru(3)O(OAc)(6) cluster core with abpy or abcp in an mu-eta(1)(N),eta(2)(N,N) bonding mode. Reduction of 3 by hydrazine induces isolation of one-electron reduced neutral Ru(3)(III,II,II) product Ru(3)O(OAc)(5){mu-eta(1)(N),eta(2)(N,N)-abcp}(py)(2) (3a). As revealed by electrochemical and spectroscopic studies, substituting one of the bridging acetates in the parent Ru(3)O(OAc)(6) cluster core by abcp or abpy modifies dramatically the electronic and redox characteristics in the triruthenium derivatives. Relative to that for the parent compound [Ru(3)O(OAc)(6)(py)(3)](PF(6)) (E(1/2) = -0.46 V), triruthenium-based redox potential in the redox process Ru(3)O(III,III,III)/Ru(3)O(III,III,II) is significantly anodic-shifted to E(1/2) = +0.36 V for 2 and E(1/2) = +0.53 V for 3. Furthermore, the anodic shifts of redox potentials are progressively enhanced with a decrease of the formal oxidation states in the triruthenium cluster cores. As a consequence of remarkable positive shifts for redox potentials, the low-valence Ru(3)(III,III,II) and Ru(3)(III,II,II) species are stabilized and accessible.

This paper proposed a new blind equalization algorithm based on Cyclostationarity. Firstly analyzed the received signal processed by fractional sample, and then presented the novel algorithm based on second order statistics (SOS) and MMSE criterion. The advantage of this algorithm is less complexity than the traditional algorithms, and it's antinoise performance is better than the traditional equalizer based zero-force criterion.

This paper presents a new blind equalization algorithm based on the second-order statistics (SOS). The equalization of the single-input multiple-output(SIMO) system can be obtained by fractionally sampled(FS). Due to fractionally sampling, the equivalent channel matrix possesses a particular structure which enables us to estimate the channel based only on the information contained in the covariance matrix at zero delay. According to different delay, the covariance matrix can be used to seek equalizer matrix with different delay, then take use of the minimum mean square error(MMSE) criterion to optimize the best delay covariance matrix of the SOS equalizer. Simulation results showed that the equalizer with optimized delay has better performance than other algorithms.

A remarkable cluster-cluster interaction is operative in dimers of delocalized Ru3O(OAc)5(py)2 clusters with ortho-metallated 2,2'-bipyrimidine (bpym) in an unprecedented mu4-eta1(C),eta1(C),eta2(N,N),eta2(N,N) bonding mode; interconversion of the dimeric species [{Ru3O(OAc)5(py)2}2(micro4-bpym)]n+ (n = 0, 1 or 2) with different charges is attainable by chemical oxidation or reduction.

Incorporation of diplatinum complex Pt2(micro-dppm)2(bpyC[triple bond]C)4 or Pt2(mu-dppm)2(phenC[triple bond]C)4 with Ln(hfac)3(H2O)2 (Ln = Nd, Eu, Yb) gave a series of Pt2Ln2 and Pt2Ln4 bimetallic arrays, in which the excitation of d(Pt) --pi*(R-C[triple bond]C) MLCT absorption induces sensitisation of lanthanide luminescence through efficient d --f energy transfer from Pt(II) alkynyl chromophores.

Reaction of oxo-centered triruthenium precursor compound [Ru3O(OAc)6(py)2(CH3OH)](PF6) (1) with 1.3 equiv of bipyridine ligand at ambient temperature gave oxo-centered triruthenium derivatives [Ru3O(OAc)5{micro-eta1(C),eta2(N,N)bipyridine}(py)2](PF6) (bipyridine = 4,4'-dibutyl-2,2'-bipyridine (dbbpy) (2),4,4'-dimethyl-2,2'-bipyridine (dmbpy) (3), 2,2'-bipyridine (bpy) (4), 5,5'-dibromo-2,2'-bipyridine (Br2bpy) (5), 1,10-phenanthroline (phen) (6)). Formation of compounds 2-6 involved substitution of the axial methanol and one of bridging acetates in the precursor compound 1 by an orthometalated bipyridine. Reduction of 2 and 4 by addition of excess hydrazine gave one-electron-reduced neutral products Ru3O(OAc)5(py)2{micro-eta1 (C),eta2(N,N)-bipyridine} (bipyridine = dbbpy (2a), bpy (4a)). As established in the structure of 3 by X-ray crystallography, the orthometalated 2,2'-bipyridine adopts a micro-eta1(C),eta2(N,N) bonding mode. In the 1H NMR spectra of 2-6, the protons of acetate, pyridine, and bipyridine show obvious paramagnetic shifts. Tentative assignments of these proton signals were carried out. Absorption spectra of the bipyridine triruthenium derivatives show characteristic intracluster charge transfer (IC) transitions in the visible to near-infrared region (600-1000 nm) and cluster-to-ligand charge transfer (CLCT) transitions at 320-450 nm. By comparison of the redox data for 2-6, it is concluded that introducing electron-donating substituents to the bipyridine favors stabilizing the [RuIII3]+ and [RuIII2RuII]0 states against disproportionation.

Reaction of Pt(diimine)(edt) (edt = 1,2-ethanedithiolate) with M(2)(dppm)(2)(MeCN)(2)(2+) (dppm = bis(diphenylphosphino)methane) gave heterotrinuclear complexes [PtCu(2)(edt)(mu-SH)(dppm)(3)](ClO(4)) (11) and [PtCu(2)(diimine)(2)(edt)(dppm)(2)](ClO(4))(2) (diimine = 2,2'-bpyridine (bpy), 12; 4,4'-dibutyl-2,2'-bipyridine (dbbpy), 13; phenanthroline (phen), 14; 5-bromophenanthroline (brphen), 15) when M = Cu(I). The reaction, however, afforded tetra- and trinuclear complexes [Pt(2)Ag(2)(edt)(2)(dppm)(2)](SbF(6))(2) (17) and [PtAu(2)(edt)(dppm)(2)](SbF(6))(2) (21) when M = Ag(I) and Au(I), respectively. The complexes were characterized by elemental analyses, electrospray mass spectroscopy, (1)H and (31)P NMR, IR, and UV-vis spectrometry, and X-ray crystallography for 14, 17, and 18. The Pt(II)Cu(I)(2) heterotrinuclear complexes 11-15 exhibit photoluminescence in the solid states at 298 K and in the frozen acetonitrile glasses at 77 K. It is likely that the emission originates from a ligand-to-metal charge transfer (dithiolate-to-Pt) (3)[p(S) --d(Pt)] transition for 11 and from an admixture of (3)[d(Cu)/p(S)-pi(diimine)] transitions for 12-16. The Pt(II)(2)Ag(I)(2) heterotetranuclear complexes 17 and 18 are nonemissive in the solid states and in solutions at 298 K but show photoluminescence at 77 K. The Pt(II)Au(I)(2) heterotrinuclear complexes 19-21, however, are luminescent at room temperature in the solid state and in solution. Compounds 19 and 20 afford negative solvatochromism associated with a charge transfer from an orbital of a mixed metal/dithiolate character to a diimine pi orbital.