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452 results on '"Lama G."'

154. Effect of placenta growth factor-1 on proliferation and release of nitric oxide, cyclic AMP and cyclic GMP in human epithelial cells expressing the FLT-1 receptor.

Author

Angelucci, Cristiana, Lama, Gina, Iacopino, Fortunata, Maglione, D, Sica, Gigliola, Angelucci C (ORCID:0000-0002-5177-6533), Lama G (ORCID:0000-0001-6036-3071), Iacopino F (ORCID:0000-0001-8691-6607), Sica G (ORCID:0000-0002-7231-9139), Angelucci, Cristiana, Lama, Gina, Iacopino, Fortunata, Maglione, D, Sica, Gigliola, Angelucci C (ORCID:0000-0002-5177-6533), Lama G (ORCID:0000-0001-6036-3071), Iacopino F (ORCID:0000-0001-8691-6607), and Sica G (ORCID:0000-0002-7231-9139)

Abstract

We investigated the effect of placenta growth factor-1 (P1GF-1) on cell growth and on the release of nitric oxide (NO), cyclic AMP (cAMP) and cyclic GMP (cGMP) in human malignant epithelial cells. A noteworthy increase in proliferation was induced in choriocarcinoma cells (BeWo) by P1GF-1 treatment, while breast cancer cells (CG-5) were minimally affected. Western blotting and immunocytochemistry demonstrated the expression of the P1GF-1 receptor fms-like tyrosine kinase-1 (Flt-1) in these models. NO was released in the BeWo culture medium as a result of P1GF-1 treatment, with maximal induction occurring after 6 h. Enhanced cAMP levels were observed after 80 min-6 h, while the amounts of cGMP produced were undetectable. In summary, PIGF-1 stimulates the proliferation of cell types that express Flt-1, other than endothelial cells. In BeWo cells, this effect is preceded by the induction of NO and cAMP as probable downstream effectors of Flt-1 activation.

Published
2001

155. Direct effects of GnRH agonists in human hormone-sensitive endometrial cells

Author

Sica, Gigliola, Schinzari, Giovanni, Angelucci, Cristiana, Lama, Gina, Iacopino, Fortunata, Sica G (ORCID:0000-0002-7231-9139), Schinzari G (ORCID:0000-0001-6105-7252), Angelucci C (ORCID:0000-0002-5177-6533), Lama G (ORCID:0000-0001-6036-3071), Iacopino F (ORCID:0000-0001-8691-6607), Sica, Gigliola, Schinzari, Giovanni, Angelucci, Cristiana, Lama, Gina, Iacopino, Fortunata, Sica G (ORCID:0000-0002-7231-9139), Schinzari G (ORCID:0000-0001-6105-7252), Angelucci C (ORCID:0000-0002-5177-6533), Lama G (ORCID:0000-0001-6036-3071), and Iacopino F (ORCID:0000-0001-8691-6607)

Abstract

The antiproliferative effect of two GnRH agonists (leuprorelin acetate and triptorelin), alone or combined with tamoxifen (TAM) or medroxyprogesterone acetate (MPA), on human estrogen-sensitive endometrial cancer cells (Ishikawa) was investigated. Although ineffective when tested alone in all the culture conditions used, both analogues counteracted or even suppressed the estrogen-stimulated growth of Ishikawa cells. The antiestrogenic effect of TAM or MPA was not modified by their association with high doses of the GnRH analogues, but low concentrations of triptorelin combined with MPA 10(-7) M determined a reduction in cell numbers which was greater than that obtained with the progestin or the analogue alone. In addition, analogue treatment prevented the estrogen-induced decrease in the level of estrogen receptors. Our data provide evidence that GnRH agonists can directly inhibit estrogen-stimulated endometrial cancer cell growth and suggest that they may interfere with steroid-receptor machinery.

Published
2001

158. Hormone receptors and growth factors in carcinoma of the prostate

Author

Sica, Gigliola, Lama, Gina, Angelucci, Cristiana, Zelano, Giovanni, Sica G (ORCID:0000-0002-7231-9139), Lama G (ORCID:0000-0001-6036-3071), Angelucci C (ORCID:0000-0002-5177-6533), Zelano G (ORCID:0000-0003-1315-6859), Sica, Gigliola, Lama, Gina, Angelucci, Cristiana, Zelano, Giovanni, Sica G (ORCID:0000-0002-7231-9139), Lama G (ORCID:0000-0001-6036-3071), Angelucci C (ORCID:0000-0002-5177-6533), and Zelano G (ORCID:0000-0003-1315-6859)

Abstract

The therapeutic and prognostic significance of androgen receptors in prostatic carcinoma has been examined on the basis of data obtained with the different techniques used in receptor determination. Moreover, the role of some polypeptide growth factors in the regulation of prostatic cancer growth and progression has been reviewed. Great attention has been focused on in vitro models utilized to investigate androgen receptor alterations and the effects of the different positive and negative regulators of prostatic carcinoma cell growth.

Published
1997

161. Effect of natural beta-interferon on estrogen receptor mRNA of breast cancer cells

Author

Sica, Gigliola, Angelucci, Cristiana, Iacopino, Fortunata, Lama, Gina, Robustelli della Cuna, G, Serlupi-Crescenzi, O, Donini, S, Sica G (ORCID:0000-0002-7231-9139), Angelucci C (ORCID:0000-0002-5177-6533), Iacopino F (ORCID:0000-0001-8691-6607), Lama G (ORCID:0000-0001-6036-3071), Sica, Gigliola, Angelucci, Cristiana, Iacopino, Fortunata, Lama, Gina, Robustelli della Cuna, G, Serlupi-Crescenzi, O, Donini, S, Sica G (ORCID:0000-0002-7231-9139), Angelucci C (ORCID:0000-0002-5177-6533), Iacopino F (ORCID:0000-0001-8691-6607), and Lama G (ORCID:0000-0001-6036-3071)

Abstract

We have demonstrated that natural beta-interferon (beta-IFN) enhances estrogen receptor (ER) mRNA of a human breast cancer cell line, CG-5. Cells were sensitive to the effect of beta-IFN at concentrations ranging from 10 to 100 IU/ml. The increase of ER mRNA was seen after 48 hr of treatment in at least three separate experiments. Our results are in agreement with the previously observed enhancement of receptor protein. In addition, they suggest that the IFN-induced promotion of the antiproliferative activity of drugs which act via ER may be due, in part, to increased receptor synthesis.

Published
1992

167. Ciclosporine (CsA) vs Cyclophosphamide (Cyc) in children with steroid-dependentfrequently relapsing nephrotic syndrome (SD/FR NS): Evaluation of growth 08320215 V3

Author

Edefonti, A., primary, Ghio, L., additional, Rizzoni, G., additional, Gusmano, R., additional, Lama, G., additional, Zacchello, G., additional, Andreucci, V., additional, Castellani, A., additional, Cinotti, G., additional, Bettinelli, A., additional, Ponticelli, C., additional, and Della Casa-Alberighi, O., additional

Published
1992
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168. Treating homeless clients with severe mental illness and substance use disorders: costs and outcomes.

Author

Morse GA, Calsyn RJ, Klinkenberg WD, Helminiak TW, Wolff N, Drake RE, Yonker RD, Lama G, Lemming MR, and McCudden S

Abstract

This study compared the costs and outcomes associated with three treatment programs that served 149 individuals with dual disorders (i.e., individuals with co-occurring severe mental illness and substance use disorders) who were homeless at baseline. The three treatment programs were: Integrated Assertive Community Treatment (IACT), Assertive Community Treatment only (ACTO), and standard care (Control). Participants were randomly assigned to treatment and followed for a period of 24 months. Clients in the IACT and ACTO programs were more satisfied with their treatment program and reported more days in stable housing than clients in the Control condition. There were no significant differences between treatment groups on psychiatric symptoms and substance use. The average total costs associated with the IACT and Control conditions were significantly less than the average total costs for the ACTO condition. [ABSTRACT FROM AUTHOR]

Published
2006
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171. Neurofibromatosis type 1 and optic pathway glioma. A long-term follow-up

Author

Lama, G., Esposito Salsano, M., Grassia, C., Calabrese, E., Grassia, M. G., Bismuto, R., Mariarosa AB Melone, Russo, S., Scuotto, A., Lama, G., ESPOSITO SALSANO, M., Grassia, C., Calabrese, E., Grassia, M., Bismuto, R., Melone, Mariarosa Anna Beatrice, Russo, S., Scuotto, Assunta, Lama, G, ESPOSITO SALSANO, M, Grassia, C, Calabrese, E, Grassia, MARIA GABRIELLA, Bismuto, R, Melone, Ma, Russo, S, and Scuotto, A.

Subjects
Adult, Male, Neurofibromatosis 1, Time Factors, neuroimaging, Adolescent, Remission, Spontaneous, Infant, optic nerve glioma, Magnetic Resonance Imaging, Severity of Illness Index, eye diseases, neurofibramotosis type 1, Child, Preschool, Optic Chiasm, Prevalence, Humans, Female, Visual Pathways, Intracranial Hypertension, Child, Neurofibromatosis type 1, Follow-Up Studies, Retrospective Studies
Abstract

AIM: Optic pathway gliomas (OPG) are the predominant intracranial tumours associated with neurofibromatosis type 1 (NF1). The aim of this study was to evaluate the prevalence and the outcome of OPG in 200 NF1 patients (122 males and 78 females, aged 1-25 years) followed up to 16 years (mean of 6 years). METHODS: All children were evaluated by a detailed physical, neurological and ophthalmological examination. Fifteen out of 200 (7.5%) of these patients (7 males, 8 females) were identified with evidence of optic pathway tumours. RESULTS: Nine children had symptoms such as endocranial hypertension, seizures, headache; 4 patients only showed anomalies at ophthalmological examination; 2 patients had no symptoms or signs. All children had evidence of optic pathway tumour on magnetic resonance imaging. Three had a prechiasmal tumour, 2 had a chiasmal tumour, 1 had prechiasmal/chiasmal tumour, 2 had a prechiasmal/chiasmal and postchiasmal tumour, 2 had a chiasmal and postchiasmal tumour, 4 had a massive involvement of the optic system, 1 child exhibited a bilateral involvement of the optic nerves with additional impairment of the chiasm. Four patients had partial and/or subtotal spontaneous regression. CONCLUSIONS: Because optic pathway tumours arise in children younger than 6 years of age, all NF1 children should undergo yearly ophtalmologic examination and growth assessment to monitor signs of precocious puberty.

177. [The role of zinc in childhood]

Author

REA F, LAMA G, PERRONE, Laura, Rea, F, Lama, G, and Perrone, Laura

Subjects
Zinc, Child, Preschool, Age Factors, Nutritional Requirements, Humans, Infant, Child
Published
1981

178. Increased serum calcitonin, reduced serum 1,25-(OH) 2-vitamin D and normal parathormone concentrations in idiopathic hypercalciuria

Author

Lama, G., Stile, V., Ragone, G., Meninno, V., Senatore, S. N., Russo, A., Lepore, A., Scopacasa, F., Iafusco, D., Arnaldo CARBONE, Lama, G, Stile, V, Ragone, G, Meninno, V, Senatore, Sn, Russo, A, Lepore, A, Scopacasa, F, Iafusco, Dario, and Carbone, A.

Subjects
Calcitonin, Male, Calcitriol, Intestinal Absorption, Parathyroid Hormone, Humans, Calcium, Female, Kidney Diseases, Child
Abstract

Twelve children (5 males and 7 females, mean age 8.5 years) with idiopathic hypercalciuria (IH), 9 with absorptive IH (1 type I and 8 type II) and 3 with renal IH, were followed in our outpatient hospital from September 1981 to March 1987. Mean (+/- 1 SD) free diet calciuria was 5.9 +/- 1.66 mg/kg/day. Diagnosis was made measuring urinary Ca/Cr ratio after controlled diet and calcium loading test. Serum parathormone (PTH), 1,25-(OH)2-D3 and calcitonin (TCT) values and renal threshold phosphate concentration (TmPO4/GFR) were also studied. Serum PTH was normal in all children. Serum TCT levels were significantly increased in the patients with IH compared with controls (p less than 0.001) while serum 1,25-(OH)2-D3 levels were significantly reduced compared with controls (p less than 0.001).

Published
1988

181. Neurofibromatosis type 1 in children: 10 years of experience

Author

Lama, G., Grassia, C., Avino, G., Salsano, Me, Calabrese, E., Tarallo, MR, Riccio, V., Cantone, D., Conforti, R., Costagliola, C., Mariarosa AB Melone, Scuotto, A., Lama, G, Grassia, C, Avino, G, ESPOSITO SALSANO, M, Calabrese, E, Tarallo, Mr, Riccio, V, Cantone, Daniela, Conforti, Renata, Costagliola, C, Melone, Mariarosa Anna Beatrice, and Scuotto, Assunta

Subjects
KeyWords Plus: GENE, IDENTIFICATION, DIAGNOSIS, CHILDHOOD, neurofibromatosis type 1, optic pathway glioma, neurofibromas, scoliosis, UBOs, learning disabilitie

182. Thyroid function in uremic children

Author

Ng, Santo, Rn, Fine, Carella C, Leumann E, Amato G, Fine S, Nuzzi F, Giovambattista Capasso, Capodicasa G, Lama G, DE SANTO, Ng, Fine, R, Carella, C, Leumann, E, Amato, G, Fine, S, Nuzzi, F, Capasso, Giovambattista, Capodicasa, G, and Lama, G.

Subjects
Thyroid Hormones, Adolescent, Peritoneal Dialysis, Continuous Ambulatory, Renal Dialysis, Thyroid Gland, Humans, Kidney Failure, Chronic, Thyroid Function Tests, Child, Uremia
Abstract

Abnormalities in thyroid function have been reported in adults with renal failure. Few data are available for uremic children and, in general, they are similar to data obtained in adults, although conflicting results on FT4have been reported. Variations in data may reflect differences in patient selection, differing ages and degrees of renal function of patients, and on different forms of dialysis, as well as laboratory methods. The objectives of this study were to assess thyroid function in uremic children with varying degrees of renal impairment and receiving different replacement therapies; to use accurate measurements of FT4; and to use age-related normal data.

187. Tamoxifen, but not estradiol, modulates the growth of a human melanoma cell line

Author

Lama, G., Sica, G., Bruzzese, N., Nori, S. L., Stefania D'Atri, Turriziani, M., and Bonmassar, E.

Subjects
cell culture, melanoma cell, tamoxifen, human cell, article, antineoplastic activity, progesterone receptor, cell proliferation, female, diethylstilbestrol, estradiol, estrogen receptor, progesterone receptor, tamoxifen, antineoplastic activity, article, cell culture, cell growth, cell proliferation, controlled study, female, human, human cell, melanoma cell, estradiol, cell growth, diethylstilbestrol, controlled study, human, estrogen receptor

188. The Italian Pilot project of the NF Register

Author

Balestrazzi, P., Lorenzetti, M. E., Sigorini, M., Lama, G., Avino, G., Rossi, L. N., Zanolini, C., Perniola, T., Margari, L., Panizon, F., Rabusin, M., Pavone, L., Ruggieri, M., Micheli, R., Gitti, F., Cacciari, E., Bergamaschi, R., Boscherini, B., Galasso, C., Franzoni, E., Migliore, M. R., Giannoti, A., Digilio, M. C., Bonioli, E., Bellini, C., Martini, C., Savasta, S., Gabrielli, O., Pellegrini, C., Iwaneiko, G., Gennari, G., Lanzi, G., Zambrino, C. A., Banchini, G., Garavelli, L., Strisciuglio, P., Corrado Romano, Zelante, L., Pasquinelli, A., Da Pelo, A., Biasini, G. C., Casadei, G. P., Comellini, L., Tozzola, A., Bassanetti, F., Busti, G., Scorza, P., Ciatto, C., and Pallotta, R.

191. The pilot project of the Italian Neurofibromatosis Register | Il progetto pilota di Registro Italiano delle Neurofibromatosi

Author

Balestrazzi, P., Lorenzetti, M. E., Sigorini, M., Lama, G., Avino, G., Rossi, L. N., Zanolini, C., Perniola, T., Lucia Margari, Panizon, F., Rabusin, M., Pavone, L., Ruggieri, M., Micheli, R., Gitti, F., Cacciari, E., Bergamaschi, R., Boscherini, B., Galasso, C., Franzoni, E., Migliore, M. R., Giannotti, A., Digilio, M. C., Bonioli, E., Bellini, C., Manini, C., Savasta, S., Gabrielli, O., Pellegrini, C., Iwaneiko, G., Gennari, G., Lanzi, G., Zambrino, C. A., Banchini, G., Garavelli, L., Strisciuglio, P., Romano, C., Zelante, L., Pasquinelli, A., Da Pelo, A., Biasini, G. C., Casadei, G. P., Comellini, L., Tozzola, A., Bassanetti, F., Busti, G., Scorza, P., Ciatto, C., Vullo, C., Banin, P., and Loi, M.

192. The pilot project of the Italian Neurofibromatosis Register,Il progetto pilota di Registro Italiano delle Neurofibromatosi

Author

Balestrazzi, P., Lorenzetti, M. E., Sigorini, M., Lama, G., Avino, G., Rossi, L. N., Zanolini, C., Perniola, T., Margari, L., Panizon, F., Rabusin, M., Pavone, L., Ruggieri, M., Micheli, R., Gitti, F., Cacciari, E., Bergamaschi, R., Boscherini, B., Galasso, C., Franzoni, E., Migliore, M. R., Giannotti, A., Digilio, M. C., Bonioli, E., Bellini, C., Manini, C., Savasta, S., Gabrielli Orazio, Pellegrini, C., Iwaneiko, G., Gennari, G., Lanzi, G., Zambrino, C. A., Banchini, G., Garavelli, L., Strisciuglio, P., Romano, C., Zelante, L., Pasquinelli, A., Da Pelo, A., Biasini, G. C., Casadei, G. P., Comellini, L., Tozzola, A., Bassanetti, F., Busti, G., Scorza, P., Ciatto, C., Vullo, C., Banin, P., and Loi, M.

194. The pilot project of the Italian Neurofibromatosis Register [Il progetto pilota di Registro Italiano delle Neurofibromatosi]

Author

Balestrazzi, P., Lorenzetti, Me, Sigorini, M., Lama, G., Avino, G., Rossi, Ln, Zanolini, C., Permiola, T., Margari, L., Panizon, F., Pavone, L., Ruggieri M, Micheli, R., Gitti, F., Cacciari, E., Bergamaschi, R., Boscherini, B., Galasso, C., Franzoni, E., Migliore, Mr, Giannotti, A., Digilio, Mc, Bonioli, E., Bellini, C., Manini, C., Savasta, S., Gabrielli, O., Pellegrini, C., Iwaneiko, G., Gennari, G., Lanzi, G., Zambrino, Ca, Banchini, G., Caravelli, L., Strisciuglio, P., Corrado Romano, Zelante, L., Pasquinelli, A., Da Pelo, A., Biasini, Gc, Casadei, Gp, Comellini, L., Tozzoli, A., Bassanetti, F., Busti, G., Scorza, P., Ciatto, C., Vullo, C., Banin, P., and Loi, M.

196. Proliferating fibroblasts and HeLa cells co-cultured in vitro reciprocally influence growth patterns, protein expression, chromatin features and cell survival

Author

Delinasios, J. G., Angeli, F., Koumakis, G., Kumar, S., Kang, W. -H, Sica, G., Iacopino, F., Lama, G., Lamprecht, S., Sigal-Batikoff, I., Tsangaris, G. T., Farfarelos, C. D., Farfarelos, M. C., Vairaktaris, E., stavros vassiliou, and Delinasios, G. J.

Subjects
cell affinity, Cell Survival, Cell Communication, In Vitro Techniques, Fibroblasts, co-culture, Chromatin, Coculture Techniques, HeLa, Tumor Microenvironment, Humans, Settore BIO/17 - ISTOLOGIA, Stromal Cells, cell-cell interactions, tumour microenvironment, Cell Proliferation, HeLa Cells
Abstract

to identify biological interactions between proliferating fibroblasts and HeLa cells in vitro.Fibroblasts were isolated from both normal and tumour human tissues. Coverslip co-cultures of HeLa and fibroblasts in various ratios with medium replacement every 48 h were studied using fixed cell staining with dyes such as Giemsa and silver staining, with immunochemistry for Ki-67 and E-cadherin, with dihydrofolate reductase (DHFR) enzyme reaction, as well as live cell staining for non-specific esterases and lipids. Other techniques included carmine cell labeling, autoradiography and apoptosis assessment.Under conditions of feeding and cell: cell ratios allowing parallel growth of human fibroblasts and HeLa cells, co-cultured for up to 20 days, a series of phenomena occur consecutively: profound affinity between the two cell types and exchange of small molecules; encircling of the HeLa colonies by the fibroblasts and enhanced growth of both cell types at their contact areas; expression of carbonic anhydrase in both cell types and high expression of non-specific esterases and cytoplasmic argyrophilia in the surrounding fibroblasts; intense production and secretion of lipid droplets by the surrounding fibroblasts; development of a complex net of argyrophilic projections of the fibroblasts; E-cadherin expression in the HeLa cells; from the 10th day onwards, an increasing detachment of batches of HeLa cells at the peripheries of colonies and appearance of areas with many multi-nucleated and apoptotic HeLa cells, and small HeLa fragments; from the 17th day, appearance of fibroblasts blocked at the G2-M phase. Co-cultures at approximately 17-20 days display a cell-cell fight with foci of (a) sparse growth of both cell types, (b) overgrowth of the fibroblasts and (c) regrowth of HeLa in small colonies. These results indicate that during their interaction with HeLa cells in vitro, proliferating fibroblasts can be activated against HeLa. This type of activation is not observed if fibroblast proliferation is blocked by contact inhibition of growth at confluency, or by omitting replacement of the nutrient medium.The present observations show that: (a) interaction between proliferating fibroblasts and HeLa cells in vitro drastically influences each other's protein expression, growth pattern, chromatin features and survival; (b) these functions depend on the fibroblast/HeLa ratio, cell topology (cell-cell contact and the architectural pattern developed during co-culture) and frequent medium change, as prerequisites for fibroblast proliferation; (c) this co-culture model is useful in the study of the complex processes within the tumour microenvironment, as well as the in vitro reproduction and display of several phenomena conventionally seen in tumour cytological sections, such as desmoplasia, apoptosis, nuclear abnormalities; and (d) overgrown fibroblasts adhering to the boundaries of HeLa colonies produce and secrete lipid droplets.

200. Literature Abstract.

Author

Lama, G., Luongo, I., Piscitelli, A., and Salsano, M.E.

Subjects
*ANGIOTENSIN converting enzyme, *NEPHROTIC syndrome in children, *PROTEINURIA
Abstract

Focuses on the antiproteinuric effect of angiotensin converting enzyme-I or enalapril in children with nephrotic syndrome. Increase of albumin levels after the treatment; Stability of creatinine clearance; Reduction of proteinuria.

Published
2001

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