Search

Additional file 3. Quality inspection results of the samples.

Background: TNF-α has been reported to be closely associated with autoimmune inflammatory diseases. This study aims to investigate the role of TNF-α -308(rs1800629) G/A gene polymorphisms as well as neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in predicting the efficacy and safety of TNF inhibitors (TNFi) in patients with ankylosing spondylitis (AS), rheumatoid arthritis (RA), and psoriasis arthritis (PsA).Methods: A total of 515 subjects (181 AS, 144 RA, 48 PsA, 10 hyperbilirubinemia, 10 hyperlipidemia and 122 healthy control) were recruited in this study. The accuracy of RT-PCR methods for identifying individual TNF-α -308 genotypes was assessed using sequencing as the gold standard. Baseline NLR and PLR of patients with AS, RA and PsA and healthy controls (HC) were calculated and compared. Meanwhile, differences between responders and non-responders to TNFi treatment as well as between individuals with and without adverse effects (AE) among responders were compared.Results: The RT-PCR method is stable and reliable for TNF-α -308G/A gene polymorphism analysis, independent of sample status. The GG genotype was overwhelmingly represented, with relatively few GA genotype, whilst the AA genotype was not detected in this study. There was no observed association between TNF-α-308G/A polymorphism and susceptibility in AS, RA or PsA patients. Patients with AS, RA, and PsA had a higher NLR, compared to the HC group. Apart from PsA patients, AS and RA patients had a higher PLR, compared to the HC group. NLR was positively correlated with PLR. Furthermore, a lack of response was more frequently observed in AS and RA patients that carrying the GA genotype than the GG genotype. AS and RA patients with AE had higher NLR and PLR, compared with the non-AE group.Conclusion: Our study preliminarily shown that combining TNF-α -308G/A polymorphisms with NLR and PLR can predict the responsiveness and safety of anti-TNF therapy in patients with AS or RA.

Additional file 2. The sequences of miRNA mimics, miRNA inhibitor, scrambled miRNA, Map4k4 wild-type and mutant.

Additional file 5. The the number of predicted target genes of differentially expressed miRNAs (MT-CCD).

Background: Stage IV breast cancer was considered to be an incurable disease with a poor survival outcome. Among them, patients with de novo stage IV breast cancer (BC) account for approximately 25%. Systemic therapy has remained the cornerstone of treatment, and the role of local therapy (Surgical resection of the primary tumor and/or radiotherapy) for de novo stage IV BC remains controversial. Aim: This study was conducted to examine the clinical and pathological profile and survival outcomes of patients with de novo stage IV BC treated with local therapy at our institutions. Settings and design: The study was conducted at two Chinese tertiary hospitals and was retrospective in nature. Methods and patients: All de novo stage IV BC patients (all female) treated with local therapy and systemic therapy alone at our hospitals between March 2007 and November 2016 were enrolled in the study. Data were retrospectively extracted from the patients’ case records. Data were analyzed according the clinicopathological features and treatment outcomes. Overall survival was used as the primary study outcome. Univariable and multivariable Cox regression analysis were used to assess the association between local therapy and overall survival. Further stratified survival analysis was used to assess the effect of surgical resection of the primary tumor before or after systemic therapy on survival outcomes. Kaplan-Meier method was used for survival analysis and the outcome variables were compared using the log-rank test. Results: A total of 138 cases of de novo stage IV BC patients admitted to our institutions were included, of whom 80 underwent local treatment. There was no significant difference in age, primary tumor N-stage, Ki-67 index, ER/PR status, and HER-2 expression between local treatment group and systemic therapy group. But there were differences in tumor size and metastatic site between the two groups. Surgical resection of the primary tumor was performed in 71/138 (51.4%) patients and 26 cases of the patients had combined radiotherapy. The median duration of follow-up was 31±20.6 months. Median survival time of all patients was 32.1 months, and the 3-year and 5-year survival rates were 36.2% and 12.3%, respectively. The median survival time of local treatment group and systemic therapy group were 38.0 and 19.0 months (HR 0.50; 95% CI 0.34-0.73; p Conclusions: Our data support that local therapy mainly based on surgical resection of the primary tumor can improve overall survival in patients with de novo stage IV BC, but fail to demonstrate the survival benefit from radiotherapy alone. However, prospective evidence from phase-3 randomized controlled trials on the feasibility of local therapy in de novo stage IV BC is required before its routine use can be recommended. Key words: De novo stage IV breast cancer; Local therapy; Surgical resection of the primary tumor; Survival outcomes Research protocol number: 2017-AF29-058 Citation Format: Ren Chongxi, Sun Jianna, Kong Lingjun, Liu Hong. Local therapy and its association on survival outcomes in patients with de novo stage IV breast cancer: Results from a retrospective cohort study [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P1-20-19.