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In radiation therapy, calculation of dose within the patient contains inherent uncertainties, inaccuracies, limitations, and the potential for random error. Thus, point dose‐independent verification of such calculations is a well‐established process, with published data to support the setting of both action levels and tolerances. Mobius3D takes this process one step further with a full independent calculation of patient dose and comparisons of clinical parameters such as mean target dose and voxel‐by‐voxel gamma analysis. There is currently no published data to directly inform tolerance levels for such parameters, and therefore this work presents a database of 1000 Mobius3D results to fill this gap. The data are tested for normality using a normal probability plot and found to fit this distribution for three sub groups of data; Eclipse,iPlan and the treatment site Lung. The mean (μ) and standard deviation (σ) of these sub groups is used to set action levels and tolerances at μ ± 2σ and μ ± 3σ, respectively. A global (3%, 3 mm) gamma tolerance is set at 88.5%. The mean target dose tolerance for Eclipse data is the narrowest at ± 3%, whilst iPlan and Lung have a range of −5.0 to 2.2% and −1.8 to 5.0%, respectively. With these limits in place, future results failing the action level or tolerance will fall within the worst 5% and 1% of historical results and an informed decision can be made regarding remedial action prior to treatment.

Figure S1. Depth of coverage summarised for the non-bisulphite treated samples per extended bait sequence reference contig. Reference extended bait sequence contigs here are organized using POPseq chromosomal pseudomolecules. a) Displays data for the NBTS sample and b) displays data for the NBTF sample. (PDF 1425Â kb)

Table S1. Drought tolerance associated genes. 120-mer probes were tiled end-to-end across these genes of particular interest [15â 18]. (PDF 81Â kb)

Figure S2. Design of the 12 Mbp wheat gene capture array. The 110 Mbp design target sequence for the capture probe set is as described by Gardiner et al. (Gardiner et al., 2015). The RNA baits for this SureSelect Methyl-Seq Target Enrichment system are all 120Â bp in length, unique, non-repetitive and are evenly placed across the available wheat genic target sequence according to the design illustrated. (PDF 187Â kb)

Shiga-toxigenic bacteriophages are converting lambdoid phages that impart the ability to produce Shiga toxin to their hosts. Little is known about the function of most of the genes carried by these phages or the impact that lysogeny has on the Escherichia coli host. Here we use next-generation sequencing to compare the transcriptomes of E. coli strains infected with an Stx phage, before and after triggering of the bacterial SOS response that initiates the lytic cycle of the phage. We were able to discriminate between bacteriophage genes expressed in the lysogenic and lytic cycles, and we describe transcriptional changes that occur in the bacterial host as a consequence of Stx phage carriage. Having identified upregulation of the glutamic acid decarboxylase (GAD) operon, confirmed by reverse transcription-quantitative PCR (RT-qPCR), we used phenotypic assays to establish the ability of the Stx prophage to confer a greater acid resistance phenotype on the E. coli host. Known phage regulators were overexpressed in E. coli, and the acid resistance of the recombinant strains was tested. The phage-encoded transcriptional regulator CII was identified as the controller of the acid response in the lysogen. Infection of an E. coli O157 strain, from which integrated Stx prophages were previously removed, showed increased acid resistance following infection with a nontoxigenic phage, ϕ24B. In addition to demonstrating this link between Stx phage carriage and E. coli acid resistance, with its implications for survival postingestion, the data set provides a number of other potential insights into the impact of lambdoid phage carriage on the biology of E. coli.

The author writes about the preretirement planning seminars that he has been conducting for the Canadian Ministry of Education for five years and about the selection and training of an appropriate resource facilitator for such seminars. (MF)

The author shares his experiences in presenting a program designed to increase employee awareness of the need to plan for a fulfilling retirement. (EM)

As a result of a seminar investigating the need for certification of trainers, a list of core competencies for training and development specialists was compiled. This checklist is organized into eleven areas of competency: (1) administration; (2) communication; (3) course design; (4) evaluation; (5) group dynamics process; (6) learning theory; (7) manpower planning; (8) person/organization interface; (9) teaching practice; (10) training equipment and materials; and (11) training needs analysis. For each of the competency areas, specific occupational skills are presented for four types of persons who conduct training or development programs: instructors; managers; designers; and consultants. (GDC)

Basic evaluation research of interim programs (concentrated curricular periods separated from the traditional semester) has employed three conceptual models. The three are identified, significant studies using them are outlined, and a fourth model is presented and recommended for use. (Editor/JT)

Figure S2. Read quality distribution for PacBio. (PDF 5 kb)

Figure S5. Impact of library preparation method on barcode switching probability. (ZIP 16 kb)

Figure S7. Taxonomic profiling of different platforms. (PDF 7 kb)

Figure S1. AMPLImock pipeline. (PDF 886 kb)

Figure S6. Heatmap of observed species in the single-species libraries. (PDF 32 kb)

Figure S8. OTU comparison for all platforms. (PDF 8 kb)