151. Isolation of sucrose-negativeYersinia enterocolitica biotype 3 serotype 03 strains and their pathogenicity
- Author
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Ichiro Omori, Hiroshi Fukushima, Kenji Maruyama, Seiji Kaneko, and Ko Ito
- Subjects
Serotype ,Autoagglutination ,biology ,General Medicine ,Carbenicillin ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Microbiology ,Virology ,Enterobacteriaceae ,Yersinia kristensenii ,Plasmid ,Ampicillin ,medicine ,Yersinia enterocolitica ,medicine.drug - Abstract
The sucrose-negative strains ofYersinia enterocolitica biotype 3 serotype 03 phage type 2 were isolated from cecal contents and oral cavity swabs of slaughtered pigs and from a swab of a skinner at the slaughterhouse. These organisms differed fromY. kristensenii, determined by assaying the antibiotic susceptibilities to ampicillin, carbenicillin, and cephalothin. These organisms showed positive reactions in the presence of 44 Md plasmid, a calcium dependency, autoagglutination activity, and produced diarrhea in mice and a negative reaction for pyrazinamidase activity. Plasmid digestion with restriction endonucleases isolated from this organism showed the same patterns in biotypes 3 and 4 serotype 03. Therefore, the sucrose-negative strains ofY. enterocolitica biotype 3 serotype 03 are apparently pathogenic.
- Published
- 1988
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