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This study aimed to improve the antioxidant and nitric oxide scavenging activities of ginseng marc fermented by Pediococcus acidilactici, thereby creating a biofunctional resource with improved anti-inflammatory capability. P. acidilactici was inoculated in 1% ginseng marc extract; cell viability, pH, and total titratable acidity were measured. Total phenolic and flavonoid contents were measured using Folin–Ciocalteu reagent and colorimetric method. Ferric reducing antioxidant power (FRAP), β-carotene, and sodium nitroprusside (SNP) assays were used to evaluate functionality. Polyphenols and flavonoids totaled 33.7 ± 0.4 and 10.0 ± 0.4 mg/g of solid, respectively, at 24 h fermentation. P. acidilactici had 40 nM β-galactosidase and 20 nM β-glucosidase activities. Antioxidative activities increased up to 34.5 and 10.2%, respectively, as measured via FRAP and β-carotene assays. Anti-inflammatory activity of the fermented extract—as measured via SNP assay—increased 342%, suggesting that ginseng marc fermented by P. acidilactici could be used in food or pharmaceutical industries.

Ginseng marc, a by-product of the extraction of fresh ginseng, is known to have bioactive compounds, but is frequently discarded as agriculture waste. The objectives of our study were to assess the antioxidative activity of fermented ginseng marc extract using Lactobacillus plantarum KCCM 11613P and to evaluate antibacterial activity of fermented milk with added ginseng marc extract during fermentation. After 24 h of fermentation of ginseng marc extract, the viable cell number was increased to 7.7 ± 0.1 log cfu/mL, and the pH and total titratable acidity were 4.2 ± 0.4 and 0.6% lactic acid, respectively. The total phenolic and flavonoid contents of fermented ginseng marc extract increased by 32.4 and 23.3%, respectively. Higher antioxidative activity of fermented ginseng marc extract was obtained in the β-carotene bleaching, ferric-reducing ability of plasma, and ferric thiocyanate assays than the 1,1-diphenyl-2-picrylhydrazy assay. However, the 1,1-diphenyl-2-picrylhydrazy scavenging effect decreased due to lowered pH. During production of fermented milk with ginseng, inhibition rate of Staphylococcus aureus and Escherichia coli were 9.7 and 2.3%, respectively. The present study shows the possibilities of Lactobacillus plantarum KCCM 11613P used as a fermentation strain and ginseng marc used as a functional supplement in milk.

Antioxidative and cytotoxic effects of chamomile (Matricaria chamomilla) fermented by Lactobacillus plantarum were investigated to improve their biofunctional activities. Total polyphenol (TP) content was measured by the Folin-Denis method, and the antioxidant activities were assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and β-carotene bleaching method. AGS, HeLa, LoVo, MCF-7, and MRC-5 (normal) cells were used to examine the cytotoxic effects by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. The TP content of fermented chamomile reduced from 21.75 to 18.76 mg gallic acid equivalent (mg GAE)/g, but the DPPH radical capturing activity of fermented chamomile was found to be 11.1% higher than that of nonfermented chamomile after 72 h of fermentation. Following the β-carotene bleaching, the antioxidative effect decreased because of a reduction in pH during fermentation. Additionally, chamomile fermented for 72 h showed a cytotoxic effect of about 95% against cancer cells at 12.7 mg solid/ml of broth, but MRC-5 cells were significantly less sensitive against fermented chamomile samples. These results suggest that the fermentation of chamomile could be applied to develop natural antioxidative and anticancer products.

Class I methanol masers are found to be good tracers of the interaction between outflows from massive young stellar objects with their surrounding media. Although polarization observations of Class II methanol masers have been able to provide information about magnetic fields close to the central (proto)stars, polarization observations of Class I methanol masers are rare, especially at 44 and 95 GHz. We present the results of linear polarization observations of 39 Class I methanol maser sources at 44 and 95 GHz. These two lines are observed simultaneously with one of the 21 m Korean VLBI Network telescopes in single-dish mode. Approximately 60% of the observed sources have fractional polarizations of a few percent in at least one transition. This is the first reported detection of linear polarization of the 44 GHz methanol maser. The two maser transitions show similar polarization properties, indicating that they trace similar magnetic environments, although the fraction of the linear polarization is slightly higher at 95 GHz. We discuss the association between the directions of polarization angles and outflows. We also discuss some targets having different polarization properties at both lines, including DR21(OH) and G82.58+0.20, which show the 90{\deg} polarization angle flip at 44 GHz.

We study the physical and chemical properties of the molecular clump hosting a young stellar cluster, IRAS 20160+3636, which is believed to have formed via the “collect and collapse” process. Physical parameters of the UC Hii region associated with the embedded cluster are measured from the radio continuum observations. This source is found to be a typical Galactic UC Hii region, with a B0.5 type exciting star, if it is ionized by a single star. We derive a CN/HCN abundance ratio larger than 1 over this region, which may suggest that this clump is being affected by the UV radiation from the Hii region.

A rapid and specific PCR assay for the simultaneous detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. Multiplex PCR developed in this study produced only actA, fliC, hbl, invA, ileS amplicons, but did not produce any non-specific amplicon. The primer sets successfully amplified the target genes in the multiplex PCR without any non-specific or additional bands on the other strains. The multiplex PCR assays also amplified some target genes from five pathogens, and multiplex amplification was obtained from as little as 1 pg of DNA. According to the results from the sensitivity evaluation, the multiplex PCR developed in this study detected 10 cells/mL of the pathogens inoculated in milk samples, respectively. The results suggested that multiplex PCR was an effective assay demonstrating high specificity for the simultaneous detection of five target pathogens in food system.

Summary Black rice bran was fermented with Bacillus subtilis KU3 isolated from Korean traditional food, Kimchi. Antioxidant and cytotoxic activities of the fermented black rice bran were investigated. Total phenolic and anthocyanin contents decreased from 171.54 mg GAE g−1 and 2.31 mg g−1 to 139.13 mg GAE g−1 and 2.12 mg g−1, respectively, after fermentation. Antioxidant activities determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, β-carotene bleaching and ferric thiocyanate assay were correlated with total phenolic and anthocyanin contents. Non-fermented black rice bran extract (NFBE) showed greater antioxidant activities than fermented black rice bran extract (FBE). Cytotoxic activities measured by MTT assay showed that both NFBE and FBE had over 50% activities. The cytotoxic activities of FBE against MCF-7 and HeLa cells were 71.65% and 68.07%, respectively, at 8.0 mg mL−1, but those of NFBE were lower than 50%. These results suggested that the cytotoxic activity of black rice bran improved through fermentation, while antioxidant activity reduced.

This study was performed to develop a predictive growth model of Listeria monocytogenes to ensure the safety of raw pork. The pork samples were inoculated with a cocktail of two L. monocytogenes strains ATCC 15313 and L13-2 isolated from pork and were stored at 5, 15, and 25 °C. Results were evaluated using the MicroFit program. To develop primary models, the Baranyi, modified Gompertz, and Logistic model equations were applied to the observed data. The mathematically predicted growth rate parameters were evaluated using the coefficient of determination (R2), bias factor (Bf), accuracy factor (Af), and mean square error (MSE). The Baranyi model, which showed an R2 of 0.998 and MSE of 0.006, was more suitable than the modified Gompertz and Logistic models. In validation study of secondary model, it appeared that MSE's of specific growth rate (SGR) and lag time (LT) were relatively accurate and suitable for modeling the growth of L. monocytogenes. These values indicated that the developed models were acceptable for expressing the growth of microorganisms on raw pork, which can be applied to ensure the safety of meats and to establish standards for avoiding microbial contamination.

Antioxidant and in vitro anti-inflammatory activities of citrus (Citrus unshiu) peel extracts and their acid hydrolysates were evaluated. Citrus peel extracts were extracted by subcritical water, hot water, and ethanol. Subcritical water extraction led to extract more phenolic compounds than hot water or ethanol extraction. Acid hydrolysis more than doubled the extracts’ total phenolics and flavonoids. Acid hydrolysates showed greater DPPH-radical scavenging activities and antioxidant activities, as assayed by β-carotene bleaching and ferric thiocyanate, than the initial extracts. Anti-inflammatory activities of citrus peel extracts and hydrolysates, determined by the inhibition of hyaluronidase activity, showed that the inhibition activities of hot water and ethanol extracts increased from 2.1 and 1.8% to 37.0 and 18.5%, respectively, upon acid hydrolysis; however, the anti-inflammatory activity of the subcritical water extract was not improved. These results indicated that acid hydrolysis of citrus peel extracts regardless of their extraction methods improved the antioxidative and anti-inflammatory activities.

Predictive models to describe the growth of Bacillus cereus in tteokgalbi were developed during storage under various temperatures (5–25C) and inoculation levels (2 and 3 log) to estimate the shelf life. To develop primary models, the observed data were applied to the Baranyi and Gompertz equations. The growth rate was dependent on temperature, but the effect of inoculation level on growth rate was not significant (P > 0.05). Thus, the secondary polynomial model was developed as a function of temperature. At 5C, the predicted shelf life of tteokgalbi was approximately 65.5–73.2 h. The shelf life at 15 or 25C decreased sharply during storage, and was less than 20 h during storage. All primary and polynomial secondary models were characterized by high R2 values, small root mean square error values and very ideal values of Bf and Af. These results present reliable predictive growth models describing the growth of B. cereus under various temperature conditions in tteokgalbi. Practical Applications Many traditional homemade foods have been industrialized at recent days. Tteokgalbi is the most popular processed pork product in Korea, and the acceptability of consumers is high because of its good flavor and nutritional value. During industrial production, the possibility of contamination of pathogens including Bacillus cereus can be high through manufacture and distribution. The predictive model in this study can be used to control the possibility of contamination for safety of tteokgalbi as basic information.

The pharmacological activities of Panax ginseng, Panax quinquefolium, and Panax notoginseng as herbal medicinal resources were reviewed to evaluate their biofunctional effects. P. ginseng contains relatively many kinds of ginsenoside that possess pharmacological activity compared with P. quinquefolium and P. notoginseng. However, the ratio of panaxatriol and panaxadiol of ginsenoside in P. notoginseng is higher than those of P. ginseng and P. quinquefolium. The free sugar content of P. ginseng is similar to P. quinquefolium but is different from P. notoginseng. The total free amino acid content is highest in P. ginseng and lowest in P. notoginseng. P. ginseng has the highest antioxidative effects and P. ginseng and P. quinquefoilum possess more potent cytotoxicities on A549 and SK-OV-3 cell lines than P. notoginseng. Particularly, red ginseng of P. ginseng shows the highest antidiabetic effects, suggesting that it possesses activity to enhance insulin secretion as well as to prevent a destruction of pancr...

Citrus fruit ( Citrus unshiu ) peels were extracted with hot water and then acid-hydrolyzed using hydrochloric acid. Antimicrobial activities of acid-hydrolyzed Citrus unshiu peel extract were evaluated against pathogenic bacteria, including Bacillus cereus , Staphylococcus aureus , and Listeria monocytogenes . Antilisterial effect was also determined by adding extracts at 1, 2, and 4% to whole, low-fat, and skim milk. The cell numbers of B. cereus, Staph. aureus, and L. monocytogenes cultures treated with acid-hydrolyzed extract for 12h at 35°C were reduced from about 8log cfu/mL to Bacillus cereus was more sensitive to acid-hydrolyzed Citrus unshiu peel extract than were the other bacteria. The addition of 4% acid-hydrolyzed Citrus unshiu extracts to all types of milk inhibited the growth of L. monocytogenes within 1d of storage at 4°C. The results indicated that Citrus unshiu peel extracts, after acid hydrolysis, effectively inhibited the growth of pathogenic bacteria. These findings indicate that acid hydrolysis of Citrus unshiu peel facilitates its use as a natural antimicrobial agent for food products.

Preservation of backseolgi by modified-atmosphere packaging (MAP) method for industrial production was evaluated with respect to shelf life and a prevalent flora was identified. Backseolgi was stored in low-density polyethylene (LDP), top-sealing package (TSP), and MAP packages. The number of microflora did not substantially change in all samples at 3°C for 30 days, but rapidly increased in LDP and TSP samples at 30°C. MAP was the most effective storage method. With DNA sequence analysis, it appeared that the predominant contaminant belonged to the genus Penicillium, and it was named Penicillium sp. DA.

Onion (Allium cepa L.) peels were extracted by ethanol, hot water and subcritical water (SW) extraction and their antioxidant activities were evaluated. Extraction yields of SW extraction were 4-fold higher than ethanol extraction. However, the ethanol extraction increased the total phenolics contents (327.5 mg GAE/g extract) and flavonoids contents (183.95 mg QE/g extract) in the onion peel extract. The onion peel extracts by ethanol extraction showed greater DPPH radical scavenging activities and greater antioxidant activities determined by ferric thiocyanate assay than those by hot water extraction and SW extraction at 165°C. Antioxidant activity of onion peel extract by SW extraction at 110°C was similar to that of ethanol extraction. HPLC profiles revealed that SW extraction at lower temperature (110°C) increased the concentration of quercetin. These results demonstrated that the onion peel extracts produced by SW extraction technique have great potential as a source for useful antioxidant.

Hydroponic ginseng (HPG) and soil-cultured ginseng (SCG) were extracted in 70% methanol to quantify relative content of 8 ginsenosides and polyphenolic compounds, and flavonoids to compare their antioxidative effects. Level of nitric oxide and inflammatory targets produced in LPS-stimulated RAW 264.7 cells were measured. 2-year-old HPG shoots contained highest levels of ginsenoside Rb2, Rb3, Rd, Re, and F1. Total polyphenol content was highest in shoots of HPG, followed by roots of HPG and SCG. HPG shoots had high radical scavenging activity and an elevated ability to inhibit linoleic acid oxidation. 2-year-old HPG shoots reduced nitric oxide production in RAW 264.7 cells by 47%, whereas 6-year-old SCG roots reduced it by only 21%. HPG also significantly lowered mRNA expression of iNOS, TNF-α, IL-1β, and IL-6, as determined by RT-PCR, compared to SCGs. Therefore, HPG may have potential for utilization as an alternative to SCG, because of superior antioxidant and anti-inflammatory properties.

We present molecular line and dust continuum observations of a Planck-detected cold cloud, G074.11+00.11. The cloud consists of a system of curved filaments and a central star-forming clump. The clump is associated with several infrared sources and H2O maser emission. We aim to determine the mass distribution and gas dynamics within the clump, to investigate if the filamentary structure seen around the clump repeats itself on a smaller scale, and to estimate the fractions of mass contained in dense cores and filaments. The velocity distribution of pristine dense gas can be used to investigate the global dynamical state of the clump, the role of filamentary inflows, filament fragmentation and core accretion. We use molecular line and continuum observations from single dish observatories and interferometric facilities to study the kinematics of the region. The molecular line observations show that the central clump may have formed as a result of a large-scale filament collision. The central clump contains three compact cores. Assuming a distance of 2.3 kpc, based on Gaia observations and a three-dimensional extinction method of background stars, the mass of the central clump exceeds 700 solar masses, which is roughly 25% of the total mass of the cloud. Our virial analysis suggests that the central clump and all identified substructures are collapsing. We find no evidence for small-scale filaments associated with the cores. Our observations indicate that the clump is fragmented into three cores with masses in the range [10,50] solar masses and that all three are collapsing. The presence of an H2O maser emission suggests active star formation. However, the CO lines show only weak signs of outflows. We suggest that the region is young and any processes leading to star formation have just recently begun., 17 pages, 19 figures, 3 tables

We present (sub)millimeter imaging at 0.5'' resolution of the massive star-forming region G358.93-0.03 acquired in multiple epochs at 2 and 3 months following the recent flaring of its 6.7 GHz methanol maser emission. Using SMA and ALMA, we have discovered 14 new Class II methanol maser lines ranging in frequency from 199 GHz to 361 GHz, which originate mostly from vt=1 torsionally-excited transitions and include one vt=2 transition. The latter detection provides the first observational evidence that Class II maser pumping involves levels in the vt=2 state. The masers are associated with the brightest continuum source (MM1), which hosts a line-rich hot core. The masers present a consistent curvilinear spatial velocity pattern that wraps around MM1, suggestive of a coherent physical structure 1200 au in extent. In contrast, the thermal lines exhibit a linear pattern that crosses MM1 but at progressive position angles that appear to be a function of either increasing temperature or decreasing optical depth. The maser spectral profiles evolved significantly over one month, and the intensities dropped by factors of 3.0 to 7.2, with the vt=2 line showing the largest change. A small area of maser emission from only the highest excitation lines closest to MM1 has disappeared. There are seven additional dust continuum sources in the protocluster, including another hot core (MM3). We do not find evidence for a significant change in (sub)millimeter continuum emission from any of the sources during the one month interval, and the total protocluster emission remains comparable to prior single dish measurements., 10 pages, 2 figures, 3 tables, accepted for publication in The Astrophysical Journal Letters. All maser transitions detected in this object can be displayed via an option on the advanced interface of http://splatalogue.net

The aim of this study was to evaluate the antimicrobial efficacy of fermented Inula britannica extract (FIBE) against Staphylococcus aureus strains including methicillin-resistant S. aureus (MRSA). I. britannica extract was fermented by Lactobacillus plantarum KCCM 11613P, and the pathogenicity of S. aureus strains was determined via assessment of coagulase, DNase, and hemolytic activities. Epicatechin concentration increased from 4.38 to 6.05 μg/mg during fermentation (p < 0.01). FIBE treatment inhibited coagulase release from S. aureus to levels below the inhibitory concentration. FIBE promoted the release of intracellular nucleic acids and N-phenyl-1-naphthylamine absorption. In three S. aureus strains, damaged cells exhibited 21.58, 16.79, and 17.65% decreases in membrane potential induced by cell membrane depolarization, respectively (p < 0.05). Upon FIBE treatment in culture, the minimum inhibitory concentration of FIBE exerted a bacteriostatic effect. In conclusion, FIBE possesses antimicrobial properties, including inhibition of virulence factors, damage to cell membranes, and inhibition of bacterial growth. PRACTICAL APPLICATIONS: Methicillin-resistant Staphylococcus aureus (MRSA) is a serious concern in hospitals because of its known antibiotic resistance. Vancomycin and tigecycline are used for treating MRSA, but the appearance of vancomycin-intermediate and multidrug-resistant strains of these bacteria has created a demand for new antimicrobial agents. This study demonstrates the effective application of Inula britannica and fermentation technology for developing natural antimicrobial agents against methicillin-resistant Staphylococcus aureus.

Correction for ‘High-detectivity perovskite-based photodetector using a Zr-doped TiOx cathode interlayer’ by C. H. Ji et al., RSC Adv., 2018, 8, 8302–8309.

White ginseng extract (WGE) was hydrolyzed with organic acids to increase the yield of ginsenoside Rg3 which has high bio-functional activities. The condition of hydrolysis by citric acid was studied to maximize a production of ginsenoside Rg3. The total content of ginsenoside Rg3 in WGE treated with 10% citric acid at 110°C for 1 h was 10-folds greater than that of commercial extract. The antitumor and antioxidant activities of WGE and the acid-hydrolysates were evaluated via an MTT assay and the DPPH method, respectively. At 1.0 mg/mL, hydrolysates inhibited the proliferation of AGS and Hep-2 cells by more than 90%, but did not inhibit their growth. It suggests that citric acid hydrolysis enhances the content of Rg3. In addition, the acid hydrolysates evidenced a free radical scavenging activity of 80% at a concentration of 4.0 mg/mL, while WGE exhibited only a 49% free radical scavenging activity at the same concentration.

Red ginseng extract (RGE) and white ginseng extract (WGE) were treated with partially purified β-glucosidase to increase a production of minor ginsenosides. The enzyme produced from Aspergillus usamii KCTC 6954 was precipitated with (NH4)2SO4. Ginseng extracts were treated with a crude extract possessing β-glucosidase activity (1,089.2 μM/mL·min) at 60°C for 72 h. The results of HPLC showed that enzyme-treated RGE and enzymetreated WGE have increased amounts of minor ginsenosides compared to each controls implying that the ginsenoside Rb1 in WGE and RGE is converted enzymatically to Rd, F2, Rg3, and compound K. In cytotoxicity study, 2.5 mg/mL of RGE, 1.25 mg/mL of ERGE, and 5 mg/mL of WGE and EWGE were effective against the HepG2, AGS, and DLD-1, but HeLa and SK-MES-1 were not affected at any concentration. The results suggested that cytotoxicity of ginseng extracts treated with β-glucosidase were greater than that of each control against cancer cells.

The antimicrobial effect of a novel flavonoid (7-O-butylnaringenin) on Helicobacter pylori 26695, 51, and SS1 strains and its inhibitory effect on the urease activity of the strains were evaluated and compared with those of several natural flavonoids. First, various flavonoids were screened for antimicrobial activities using the paper disc diffusion method. Hesperetin and naringenin showed the strongest antimicrobial effects among the natural flavonoids tested, and thus hesperetin and naringenin were selected for comparison with 7-O-butylnaringenin. The antimicrobial effect of 7-O-butylnaringenin was greater than that of the hesperetin and naringenin. H. pylori 51 was more sensitive to 7-O-butylnaringenin (2 log reduction of colony forming units, p < 0.05) than the other two strains at 200 μM. 7-O-Butylnaringenin also showed the highest inhibitory effect against urease activity of H. pylori. Morphological changes of H. pylori 26695 treated with these flavonoids indicated that both hesperetin and 7-O-butylnaringenin at 200 μM damaged the cell membranes.

The objective of this study was to evaluate the antibacterial activity of a novel flavonoid, 7-O-butyl naringenin, as well as natural flavonoids (quercetin, naringenin) against methicillin-resistant Staphylococcus aureus (MRSA). 7-O-butyl naringenin showed great anti-MRSA, with a MIC (minimum inhibitory concentration) value of 0.625 mM. The number of cells treated with 25 μM 7-O-butyl naringenin was reduced by 5.2 log CFU/mL compared with the control (DMSO). The result indicated that 7-O-butyl naringenin was more effective than the two natural flavonoids against MRSA, and may have potential as a novel therapeutic agent for MRSA infections.

Summary Antibacterial activities of ginseng extracts produced from ginseng by-products, stems and leaves, using subcritical water extraction (SWE) at 110, 165 and 190 °C, were evaluated and compared with those of ginseng extracts prepared by hot water and ethanol extraction. The ginseng stems–leaves extract produced by SWE at 190 °C contained the greatest concentration of phenolics (98.4 mg GAE g−1 of extract). All ginseng extracts inhibited the growth of Bacillus cereus, Salmonella enteritidis, Escherichia coli O157:H7 and Listeria monocytogenes. Among four strains, B. cereus was more sensitive to the ginseng extract by SWE at 190 °C than other bacteria. Cell membranes of bacteria were disrupted by the addition of SWE ginseng extract, observed by transmission electron microscopy (TEM), with the release of cellular contents. These findings provided evidence about the potential utilisation of ginseng stems and leaves by using an environmental friendly extraction process, SWE, to produce ginseng extract for the inhibition of bacteria growth.

We analyzed both HCN J=1-0 and HNC J=1-0 line profiles to study the inflow motions in different evolutionary stages of massive star formation: 54 infrared dark clouds (IRDCs), 69 high-mass protostellar object (HMPOs), and 54 ultra-compact HII regions (UCHIIs). The inflow asymmetry in HCN spectra seems to be prevalent throughout all the three evolutionary phases, with IRDCs showing the largest excess in blue profile. In the case of HNC spectra, the prevalence of blue sources does not appear, excepting for IRDCs. We suggest that this line is not appropriate to trace inflow motion in evolved stages of massive star formation because the abundance of HNC decreases at high temperatures. This result spotlights the importance of considering chemistry in the dynamics study of massive star-forming regions. The fact that the IRDCs show the highest blue excess in both transitions indicates that the most active inflow occurs in the early phase of star formation, i.e., the IRDC phase rather than in the later phases. However, mass is still inflowing onto some UCHIIs. We also found that the absorption dips of the HNC spectra in 6 out of 7 blue sources are red-shifted relative to their systemic velocities. These red-shifted absorption dips may indicate global collapse candidates, although mapping observations with better resolution are needed to examine this feature in more detail., 36 pages, 7 figures, 5 Tables, accepted for publication in ApJS

Most bipolar outflows are associated with individual young stellar objects and have small opening angles. Here we report the discovery of an extremely wide-angle ($\sim$180$\arcdeg$) bipolar outflow ("EWBO") in a cluster forming region AFGL 5142 from low-velocity emission of the HCN (3-2) and HCO$^{+}$ (3-2) lines. This bipolar outflow is along a north-west to south-east direction with a line-of-sight flow velocity of about 3 km~s$^{-1}$ and is spatially connected to the high-velocity jet-like outflows. It seems to be a collection of low-velocity material entrained by the high-velocity outflows due to momentum feedback. The total ejected mass and mass loss rate due to both high velocity jet-like outflows and the "EWBO" are $\sim$24.5 M$_{\sun}$ and $\sim1.7\times10^{-3}$ M$_{\sun}$~yr$^{-1}$, respectively. Global collapse of the clump is revealed by the "blue profile" in the HCO$^{+}$ (1-0) line. A hierarchical network of filaments was identified in NH$_{3}$ (1,1) emission. Clear velocity gradients of the order of 10 km~s$^{-1}$~pc$^{-1}$ are found along filaments, indicating gas inflow along the filaments. The sum of the accretion rate along filaments and mass infall rate along the line of sight is $\sim$3.1$\times10^{-3}$ M$_{\sun}$~yr$^{-1}$, which exceeds the total mass loss rate, indicating that the central cluster is probably still gaining mass. The central cluster is highly fragmented and 22 condensations are identified in 1.1 mm continuum emission. The fragmentation process seems to be determined by thermal pressure and turbulence. The magnetic field may not play an important role in fragmentation., accepted by ApJ

Rb-1-hydrolyzing beta-glucosidase from Aspergillus niger KCCM 11239 was studied to develop a bioconversion process for minor ginsenosides. The specific activity of the purified enzyme was 46.5 times greater than that of the crude enzyme. The molecular weight of the native enzyme was estimated to be approximately 123 kDa. The optimal pH of the purified enzyme was pH 4.0, and the enzyme proved highly stable over a pH range of 5.0-10.0. The optimal temperature was 70 degrees C, and the enzyme became unstable at temperatures above 60 degrees C. The enzyme was inhibited by Cu2+, Mg2+, Co2+, and acetic acid (10 mM). In the specificity tests, the enzyme was found to be active against ginsenoside Rb-1, but showed very low levels of activity against Rb-2, Rc, Rd, Re, and Rg(1). The enzyme hydrolyzed the 20-C,beta-(1 -> 6)-glucoside of ginsenoside Rb-1 to generate ginsenoside Rd and Rg(3), and hydrolyzed 3-C,beta-(1 -> 2)-glucoside to generate F-2. The properties of the enzyme indicate that it could be a useful tool in biotransformation applications in the ginseng industry, as well as in the development of novel drug compounds.

Rb1-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239 was studied to develop a bioconversion process for minor ginsenosides. The specific activity of the purified enzyme was 46.5 times greater than that of the crude enzyme. The molecular weight of the native enzyme was estimated to be approximately 123 kDa. The optimal pH of the purified enzyme was pH 4.0, and the enzyme proved highly stable over a pH range of 5.0–10.0. The optimal temperature was 70 °C, and the enzyme became unstable at temperatures above 60 °C. The enzyme was inhibited by Cu2+, Mg2+, Co2+, and acetic acid (10 mM). In the specificity tests, the enzyme was found to be active against ginsenoside Rb1, but showed very low levels of activity against Rb2, Rc, Rd, Re, and Rg1. The enzyme hydrolyzed the 20-C,β-(1→6)-glucoside of ginsenoside Rb1 to generate ginsenoside Rd and Rg3, and hydrolyzed 3-C,β-(1→2)-glucoside to generate F2. The properties of the enzyme indicate that it could be a useful tool in biotransformation applications in the ginseng industry, as well as in the development of novel drug compounds.

Particle dispersion and fluctuations were investigated in a liquid–solid fluidised bed with viscous liquid media by employing the relaxation method based on the stochastic model. The bed expansion or contraction during the transient state was analysed by means of the histogram of pressure drop variation with elapsed time. The relaxation behaviour of the liquid–solid fluidised bed appeared to be heterogeneous followed by homogeneous expansion or contraction. Effects of liquid velocity (UL), particle size (dP), liquid viscosity (µL) and liquid holdup or bed porosity (eL) on the fluctuating frequency (F) and dispersion coefficient (DP) of fluidised solid particles were examined. The values of F and DP increased with an increase in dP but decreased with µL and exhibited local maxima with variations of UL and eL. The flow pattern of solid particles could be changed from uniform or pseudo-homogeneous to turbulent random behaviour with an increase in the liquid velocity and liquid holdup or bed porosity. © 2012 Canadian Society for Chemical Engineering

We have carried out simultaneous 22 GHz H2O and 44 GHz Class I CH3OH maser line surveys of more than 1500 intermediate- and high-mass YSOs in the Galaxy using newly-constructed KVN 21-m telescopes. As the central (proto)stars evolve, the detection rates of the two masers rapidly decrease for intermediate-mass YSOs while the rates increase for high-mass YSOs. These results suggest that the occurrence of the two masers is closely related both to the evolutionary stage of the central objects and to the circumstellar environments. CH3OH masers always have very similar velocities (−1) to the natal dense cores, whereas H2O masers often have significantly different velocities. The isotropic luminosities of both masers are well correlated with the bolometric luminosities of the central (proto)stars.

We have started survey observations of the 22 GHz water maser sources associated with high-mass young stellar objects (HM-YSOs) as a part of the KaVA (KVN and VERA Array) large program (LP). The aim of our LP is to understand dynamical evolution of jets/outflows from HM-YSOs by analyzing 3D velocity structures of water maser features. In the first year (2016-2017), an imaging survey toward 25 HM-YSOs has been conducted and the 22 GHz water masers are detected toward 21 sources. Spatial distributions of maser features for individual sources are mapped. To complement physical properties in the vicinity of HM-YSOs, we have carried out ALMA cycle 3 observations of thermal molecular lines and continuum emissions toward 11 selected samples. Summary of the KaVA first year observations and the initial results from the ALMA toward one of our targets, G25.82-0.17, are reported.

In this study, the antimicrobial effects of an onion peel extract prepared using subcritical water extraction (SWE) were assessed for possible development into new bio-functional materials. The extraction temperatures were controlled to 110 and 160°C. At 0.15, 0.3, 0.6, and 1.2 mg extract/mL of broth, the growth inhibition and bactericidal activity of SWE extracts against Bacillus cereus KCCM 40935 and KCCM 11341 were compared with those of ethanol and hot-water extracts. In the case of B. cereus KCCM 40935, it appeared that over 0.6 mg/mL of SWE (110°C) extract exerted a bactericidal effect, and 1.2 mg/mL of SWE (160°C) extract exerted a bacteriostatic effect during culturing, and also that B. cereus KCCM 11341 was more resistant than B. cereus KCCM 40935. Furthermore, our results demonstrated that the death time of 107 CFU/mL of B. cereus KCCM 40935 treated with SWE (110°C) extract at 1.2 mg/mL was 60 min at maximum in 0.8% NaCl. Additionally, the cells damaged by SWE extract were observed with a SEM. It was suggested that an extract of onion peels prepared via SWE (110°C) could be used as a functional biomaterial for the food or pharmaceutical industries.

The antimicrobial effects of the natural flavonoids kaempferol, quercetin, apigenin, and naringenin as well as a novel fla-vonoid 7-O-butyl naringenin against the growth of four meat-born Staphylococcus aureus strains were evaluated. First, theflavonoids were screened for inhibitory effects against the growth of each strain using the paper disc diffusion method. Sec-ond, the growth inhibitory effects of flavonoids that showed antimicrobial activity were measured using the microplatemethod. Third, the bactericidal effects of flavonoids were evaluated in a 0.8% (w/v) NaCl solution. All flavonoids showedbacteriostatic effects at >20 mM. Among the flavonoids studied, quercetin was more effective than the others tested. How-ever, the inhibitory effect of 7-O-butyl naringenin on growth of S. aureus KCCM 32395 was greater than that of quercetinat the same concentration. Additionally, 7-O-butyl naringenin exhibited significant bactericidal effects at >25 µM. Whenbacterial cells were examined using scanning electron microscopy, it appeared that the S. aureus membranes were damagedor morphologically changed when treated with quercetin and 7-O-butyl naringenin at 200 µM.Key words: flavonoid, 7-O-butyl naringenin, Staphylococcus aureus, antimicrobial effect

We carry out 100-GHz band test observations with the newly-constructed KVN 21-m radio telescopes in order to evaluate their performance. The three telescopes have similar performance parameters. The pointing accuracies are about 4 00 rms for the entire sky. The main beam sizes are about 30 00 (FWHMs), which is nearly the difiraction limit of the telescopes at the observing frequency (97 GHz). The measured aperture and main-beam e‐ciencies are about 52% and 46%, respectively, for all three telescopes. The estimated moon e‐ciency is »84% for the KVN Tamna telescope. The flrst sidelobes appear 50 00 (»1.6£FWHM) from the main beam centers and the levels are on average i14 dB.

The objective of this study was to evaluate the antimicrobial effects of various natural flavonoids against growth of psy-chotropic Bacillus cereus strains, which cause dairy food outbreaks. Flavonoids were first screened for their ability to inhibitgrowth of B. cereus strains using the paper-disc diffusion test. Second, the growth inhibitory effect of selected flavonoidswas evaluated in tryptic soy broth supplemented with 0.6% yeast extract, and the bactericidal effect of the flavonoids wasmeasured in 0.8% (w/v) NaCl solution. Based on the paper-disc diffusion test, kaempferol was effectively active against B.cereus P14 and B. cereus KCCM 40935. Kaempferol had an antimicrobial effect at concentrations greater than 100 µM, andthe numbers of B. cereus P14 and B. cereus KCCM 40935 decreased by 3.55 and 1.5 log cycles, respectively. The cell num-bers of B. cereus P14 and B. cereus KCCM 40935 treated with 50 µM kaempferol were reduced by 4.18 and 2.84 log cyclesduring a 24 h incubation to test the bactericidal effect of kaempferol ( p

The objective of this study was to evaluate the antimicrobial and antioxidative effects of the onion peels extract prepared via the subcritical water extraction (SWE) method. First, the number of cells treated with the extract was reduced by 0.7–1.1 log CFU/mL compared with the control. The SWE extract showed 76.08% scavenging activity and it was more effective than butylated hydroxytoluene (BHT) at 61.3 ppm in lipid peroxidation inhibitory effects. In addition, the antioxidative effect of SWE extract measured via the ferric thiocyanate (FTC) method was 2-fold that of BHT. The results of this study suggest that the onion peels extract prepared via the SWE method may have potential alternative antimicrobial and antioxidative effects as functional substances.

We performed a molecular line survey of 82 high-mass protostellar objects in search for inflow signatures associated with high-mass star formation. Using the H$^{13}$CO$^+$ (1$-$0) line as an optically thin tracer, we detected a statistically significant excess of blue asymmetric line profiles in the HCO$^+$ (1$-$0) transition, but nonsignificant excesses in the HCO$^+$ (3$-$2) and H$_2$CO (2$_{12}$$-$1$_{11}$) transitions. The negative blue excess for the HCN (3$-$2) transition suggests that the line profiles are affected by dynamics other than inflow motion. The HCO$^+$ (1$-$0) transition thus seems to be the suitable tracer of inflow motions in high-mass star-forming regions, as previously suggested. We found 27 inflow candidates that have at least one blue asymmetric profile and no red asymmetric profile, and derived the inflow velocities to be 0.23$-$2.00 km s$^{-1}$ for 20 of them using a simple two-layer radiative transfer model. Our sample is divided into two groups in different evolutionary stages. The blue excess of the group in relatively earlier evolutionary stages was estimated to be slightly higher than that of the other in the HCO$^+$ (1$-$0) transition., Comment: 35 pages, 4 figures, Published in ApJS

Flavonoids have biofunctional effects as antioxidant and antimicrobial agents. The objective of this study was to evaluate the bactericidal activity and antilipopolysaccharide (LPS) effects of flavonoids on Escherichia coli O157:H7. The effects of flavonoids on the growth of E. coli O157:H7 were bacteriostatic rather than bactericidal at concentrations below 200 μM, and there was no significant difference among the flavonoids tested (p

We make the first data release (DR1) of the ongoing ESO Public Survey SAMPLING (this http URL). DR1 comprises of 124 fields distributed in $70^\circ < l < 216^\circ$, $-35^\circ < b < 25^\circ$. The 12CO and 13CO (2-1) cubes are gridded in $8"$ pixels, with an effective resolution of $36"$. The channel width is 0.33 km/s and the RMS noise is $T_{\rm mb}

Listeriolysin O (LLO) is a heat-labile hemolysin produced by Listeria mono-cytogenes. Its hemolytic activity has been evaluated qualitatively by sodium dodecyl sulfate (SDS) electrophoresis and immunoblotting. In this experiment, an enzyme-linked immunosorbent assay (ELISA) was developed for quantitative analysis of LLO by using Streptolysin O (SLO) and antistreptolysin O (ASO) as the reagents. The selected coating and blocking buffers were 0.05 M Tris buffer (pH 8.5) and 0.25% casein solution with phosphate-buffered saline solution + 0.05% Tween 20 (PBS-T), respectively. A relationship between ASO and antibody was achieved with 5 mg/ml ASO and a 1:1,000 dilution of conjugate. The heat stability of LLO at 48, 62, 72, and 80C was examined by using this method and compared with a traditional hemolysis assay. Although the LLO is inactivated easily at those temperatures, the protein structure was not affected at temperatures lower than 80C for 3 min, pointing to a need for both hemolysis and ELISA to be conducted in determining both the activity and presence of LLO in foods.

We present a simultaneous single-dish survey of 22 GHz water maser and 44 GHz and 95 GHz class I methanol masers toward 77 6.7 GHz class II methanol maser sources, which were selected from the Arecibo methanol maser Galactic plane survey (AMGPS) catalog.Water maser emission is detected in 39 (51%) sources, of which 15 are new detections. Methanol maser emission at 44 GHz and 95 GHz is found in 25 (32%) and 19 (25%) sources, of which 21 and 13 sources are newly detected, respectively. We find 4 high-velocity (> 30 km/s) water maser sources, including 3 dominant blue- or redshifted outflows.The 95 GHz masers always appear with the 44 GHz maser emission. They are strongly correlated with 44 GHz masers in velocity, flux density, and luminosity, while they are not correlated with either water or 6.7 GHz class II methanol masers. The average peak flux density ratio of 95 GHz to 44 GHz masers is close to unity, which is two times higher than previous estimates. The flux densities of class I methanol masers are more closely correlated with the associated BGPS core mass than those of water or class II methanol masers. Using the large velocity gradient (LVG) model and assuming unsaturated class I methanol maser emission, we derive the fractional abundance of methanol to be in a range of 4.2*10^-8 to 2.3*10^-6, with a median value of 3.3\pm2.7*10^-7., 45 pages, 15 figures, 5 tables. Accepted for publication in ApJS

In this study, the effects of magnolia (Magnolia (M.) denudata) extract fermentation in increasing the extract’s antioxidative and anticancer activities were investigated. Magnolia was fermented by Pediococcus acidilactici KCCM 11614. The total phenolic content was determined by the Folin-Ciocalteu’s method and the antioxidative effects by 1,1-diphenyl-2-picrylhydrazy (DPPH) and ferric reducing ability of plasma (FRAP) assay. Anticancer activity against cancer and normal cells was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). Total phenolic content during fermentation increased from 38.1 to 47.0 mg gallic acid equivalent (GAE)/g of solid matter. The radical scavenging activity was 91.4% after 72 h fermentation. Fermented magnolia’s antioxidative effect was threefold higher than that of the (non-fermented) control. Fermentation (48 h) increased anticanceric activity against AGS, LoVo, and MCF-7 cancer cells 1.29- to 1.36-fold compared with that of the control, but did not affect MRC-5 (normal) cells, suggesting that fermented magnolia could be used as a natural antioxidative and anticancer agent.

Using the HCN and HNC J=1--0 line observations, the abundance ratio of HCN/HNC has been estimated for different evolutionary stages of massive star formation: Infrared dark clouds (IRDCs), High-mass protostellar object (HMPOs), and Ultra-compact HII regions (UCHIIs). IRDCs were divided into `quiescent IRDC cores' and `active IRDC cores', depending on star formation activity. The HCN/HNC ratio is known to be higher at active and high temperature regions related to ongoing star formation, compared to cold and quiescent regions. Our observations toward 8 quiescent IRDC cores, 16 active IRDC cores, 23 HMPOs, and 31 UCHIIs show consistent results; the ratio is 0.97~($\pm~0.10$), 2.65~($\pm~0.88$), 4.17~($\pm~1.03$) and 8.96~($\pm~3.32$) in these respective evolutionary stages, increasing from quiescent IRDC cores to UCHIIs. The change of the HCN/HNC abundance ratio, therefore, seems directly associated with the evolutionary stages of star formation, which have different temperatures. One suggested explanation for this trend is the conversion of HNC to HCN, which occurs effectively at higher temperatures. To test the explanation, we performed a simple chemical model calculation. In order to fit the observed results, the energy barrier of the conversion must be much lower than the value provided by theoretical calculations., Comment: 55 pages, 8 figures, accepted for publication in ApJS

W49N is a mini-starburst in the Milky Way and thus an ideal laboratory for high-mass star formation studies. Due to its large distance (11.1$_{-0.7}^{+0.9}$ kpc), the kinematics inside and between the dense molecular clumps in W49N are far from well understood. The SMA observations resolved the continuum emission into two clumps. The molecular line observation of SO$_{2}$ (28$_{4,24}$-28$_{3,25}$) suggests that the two clumps have a velocity difference of $\sim$7 km~s$^{-1}$. The eastern clump is very close to two radio sources "G1" and "G2", and the western clump coincides with a radio source "B". The HCN (3-2) line reveals an extremely energetic outflow, which is among the most energetic molecular outflows in the Milky Way. This is the first report of high-velocity molecular outflow detection in W49N. The outflow jet might be in precession, which could account for the distribution, velocity and rotation of water maser spots. Three absorption systems are identified in HCO$^{+}$ (3-2) spectra. The absorption features are blueshifted with respect to the emission of SO$_{2}$ (28$_{4,24}$-28$_{3,25}$) lines, indicating that a cold layer is expanding in front of the warm gas. Further analysis indicates that the expansion is decelerated from the geometric expansion centers., Comment: Accepted to ApJ

We investigated the antagonistic effects of two lactococcal bacteriocins, nisin or lacticin NK24, on the growth, the survival of Listeria monocytogenes in vacuum or modified atmosphere-packaged ground beef. Ground beef was inoculated with approximately 3 log colony-forming units (CFU) of L. monocytogenes ATCC 15313 culture per gram of ground beef. Inoculated samples were blended with/without 100 AU/g nisin or lacticin NK24,, subsequently vacuum or modified atmosphere packed at 4℃. Listeria in the bacteriocin-treated, control samples was subsequently isolated from both vacuum, modified atmosphere packs, enumerated as CFU on Listeria Isolation Agar medium. Microbial counts in ground beef treated with bacteriocin declined steadily, while those of non-treated beef samples increased steadily. The results obtained demonstrate that nisin inhibits the growth of L. monocytogenes more effectively than lacticin NK24 at 100 AU/g. The use of lactococcal bacteriocins, such as nisin or lacticin NK24, in vacuum or modified atmosphere packaging offers a promising approach for eliminating or reducing the risk of L. monocytogenes contamination in ground beef.

We introduce and describe performance of the 6-meter telescope of Seoul Radio Astronomy Observatory (SRAO). All the softwares and instruments except the antenna structure and its driving system are developed for ourselves. The SIS mixer type receiver resulted in the receiver noise temperature less than 50 K (DSB) over the whole 3-mm radio window. An autocorrelation spectrometer, developed first in Korea, provides maximum 50 MHz band width over 1024 channels. Antenna surface is measured and adjusted using template method and radio holography which resulted in a superb surface accuracy bet-ter than 30. Accordingly, the aperture and beam efficiences amount to and , respectively, largely independent of frequency in the 85 - 115 GHz range. It is also found that telescope pointing errors are less than 10" in both azimuth and elevation and that antenna gain is almost constant against elevation greater than , without adjusting sub-reflector position. The SRAO 6-meter telescope is now fully operational and all these characteristics verify that observations are carried out with high precision and fidelity.

Apoptosis is a common mode of programmed cell death in multicellular organisms. However, the recent observation of yeast cell death displaying the morphology of apoptosis has suggested the presence of an ancestral cell death machinery. Here we examined apoptotic features induced by reactive oxygen species (ROS) in yeast. Saccharomyces cerevisiae show typical apoptotic features upon exposure to ROS: membrane staining with annexin V and DNA fragmentation by the TUNEL assay. The detection of apoptotic features in yeast strongly support the existence of molecular machinery performing the basic pathways of apoptosis. The phosphoinositide 3-OH kinase (PI3K)/protein kinase B (PKB) signaling pathway has been shown to prevent apoptosis in a variety of cells. It is therefore of interest to determine whether the PI3K/PKB signaling pathway is capable of protecting yeast from apoptosis induced by ROS. We determined that PI3K/PKB is capable of significantly inhibiting ROS-evoked apoptosis in yeast. These results suggest that yeast may provide a suitable model system in which to study the apoptotic signaling pathway elicited by a variety of stimuli.

Oxygen-rich Asymptotic Giant Branch (AGB) stars can be intense emitters of SiO ($v$=1 and 2, J=1$\rightarrow$0) and H$_2$O maser lines at 43 and 22 GHz, respectively. VLBI observations of the maser emission provide a unique tool to probe the innermost layers of the circumstellar envelopes in AGB stars. Nevertheless, the difficulties in achieving astrometrically aligned \water\ and $v$=1 and $v$=2 SiO maser maps have traditionally limited the physical constraints that can be placed on the SiO maser pumping mechanism. We present phase referenced simultaneous spectral-line VLBI images for the SiO $v$=1 and $v$=2, J=1$\rightarrow$0, and H$_2$O maser emission around the AGB star R\,LMi, obtained from the Korean VLBI Network (KVN). The simultaneous multi-channel receivers of the KVN offer great possibilities for astrometry in the frequency domain. With this facility we have produced images with bona-fide absolute astrometric registration between high frequency maser transitions of different species to provide the positions of the \water\ maser emission, and the centre of the SiO maser emission, and hence reducing the uncertainty in the proper motion for R\,LMi by an order of magnitude over that from Hipparcos. This is the first successful demonstration of source frequency phase referencing for mm-VLBI spectral-line observations and also where the ratio between the frequencies is not an integer., Comment: Accepted in AJ

We have carried out 21 cm radio continuum observations of 16 ultracompact H II regions using the VLA (DnC array) in a search for associated extended emission. We have also observed the H76α recombination line toward all the sources and the He76α line at the positions with strong H76α line emission. The UC H II regions have simple morphologies and large (10) ratios of single-dish to VLA fluxes. We detected extended emission toward all the sources. The extended emission consists of one to several compact (~1' or 0.5-5 pc) components and a diffuse extended (2'-12' or 4-19 pc) envelope. All the UC H II regions but two are located in the compact components, where the UC H II regions always correspond to their peaks. The compact components with UC H II regions are usually smaller and denser than those without UC H II regions. For individual sources, we derive the spectral types (O7-O4) of the ionizing stars and the fractions of UV photons absorbed by dust within the nebulae, which are significantly different from previous estimates based on the UC H II regions alone. Our recombination line observations show that the ultracompact, compact, and extended components have approximately the same velocity in the individual sources with one exception (G25.72+0.05), implying that they are physically associated. The compact components in each object appear to be ionized by separate sources, while the UC H II regions and their associated compact components are likely to be ionized by the same sources on the basis of the morphological relations mentioned above. This suggests that almost all of the observed UC H II regions are not "real" UC H II regions but ultracompact cores of more extended H II regions, and that their actual ages are much greater than their dynamical age (104 yr). We find that most of simple UC H II regions previously known have large ratios of single-dish to VLA fluxes, similar to our sources. Therefore, the "age problem" of UC H II regions does not seem to be as serious as earlier studies argued. We present a simple model in which the coexistence of the ultracompact, compact, and extended components for a long (>105 yr) time is easily explained by combining the champagne flow model with the hierarchical structure of massive star-forming regions. The well-known relation between the density and diameter of H II regions, ne ∝ D-1, is a natural consequence of the hierarchical structure according to our model. We discuss some individual sources.

A sophisticated photogrammetric adjustment technique was investigated to derive accurate positional information from the digitized declassified historical satellite imagery. We used extended Gauss-Markov model to adjust observation equations for differential rectification. Results show that we can obtain about 150 m horizontal accuracy of orthorectified ARGON image, which corresponds to the horizontal resolution of the image. The result is very promising concerning very wide of coverage (540 km×540 km) of single frame film ARGON imagery whose size is 120mm×120 mm. We identified that the consideration of Earth curvature effect for input photo coordinates is essential for the accurate orthorectification using the very wide ground coverage film. Result also suggests that the usefulness of declassified historical satellite imagery for scientific studies of very wide area when they are employed a sophisticated photogrammetric technique such as differential rectification.