Search

The present study was undertaken to investigate the effect of anxiety on pulmonary function parameters and respiratory sinus arrhythmia (RSA) in healthy people under real-life conditions. This study consisted of two interrelated parts. During the first stage, eighty healthy students were examined in the following sequence: recording of heart rate variability (HRV) and respiration parameters at rest and shortly before real life stress. In a longitudinal study (the second stage), we assessed the profile of cardiorespiratory activity over 50 days in ten healthy women. Pulmonary function parameters like breath rate, tidal volume, forced expiratory volume in ones (FEV1), peak expiratory flow (PEF), forced expired flow at 25%, 50%, 75% of FVC, forced expired flow from 25-75% of FVC (FEF25-75%) and HRV measures (SDNN, RMSSD, pNN50, LF, HF, HFnorm, LF/HF ratio) of all subjects were tested. State anxiety was measured by Spielberger state anxiety inventory. Higher levels of state anxiety were associated with higher levels of breath rate, tidal volume and reduced HRV parameters, especially indicators of the RSA (HF and HFnorm) at baseline. These changes depend on the category of state anxiety: the group of students with a qualitative increase in state anxiety before examination has increased level of FEV1, PEF, forced expired flow at 25%, 50%, 75% of FVC, FEF25-75%. Less reactive students have no difference in respiratory parameters. We found a strong negative correlation between the level of HF at rest and state anxiety scores before examination. Longitudinal study found a negative correlation between RSA parameters and PEF, positive correlation between state anxiety and PEF of the majority those surveyed, except for two women with low levels of state anxiety and RSA indicators were in a narrow range. The variations of cardiorespiratory parameters during a longitudinal study depend on the changes of state anxiety. The higher level of state anxiety was associated with significant changes in RSA and the respiratory parameters. These results lend strong support to the notion that parasympathetic function is a critical physiological component of emotional processes.

We show that a fluid dynamical scenario, already well tested against identified particle p(t) spectra, describes quantitatively the observed mass splitting of the elliptical flow coefficients v(2) for pions and protons. This provides a strong argument in favor of the existence of a fluid dynamical expansion in p-Pb collisions at 5.02 TeV.

Resistance of three materials, produced on the basis of concrete compounds to the action of microscopic fungi, isolated from damaged living buildings, has been first investigated. It has been shown that samples of froth-block and thermoeffective block had low fungal resistance, in contrast to samples of cellular polystyrene concrete, which were resistant to fungal action, that can be associated with peculiarities of their component composition.

The features of radial growth of three microscopic fungi species were investigated at illumination by yellow, blue, green and red light. The studied species of fungi differed by isolation site (some of them were isolated from the places of high radioactive pollution, others--from the places with background level of radioactivity) and pigmentation degree (melanin-containing and light-colored). The parameters of radial growth, which are the integral indexes of the fungal organism physiology state were investigated: radial growth rate, branching degree and summarizing index--the intensity of substrate consumption. It was shown that the melanin-containing fungi Cladosporium cladosporioides growth was twice more rapid in response to blue and yellow light. Whereas such light depressed growth of non-pigmented strain of the same species as compared to control. A light response of other pigment-containing species Hormoconis resinae was far less expressed, and the light-colored species Paecilomyces lilacinus had no response to any offered conditions of illumination.

Functional states were studied in students in the intersession period and just before exams. It was found that there was an increase in markers of sympathetic activity and a reduction in markers of parasympathetic tone of the autonomic nervous system. A considerable individual variability in the pattern of a reaction was revealed in relation to the body mass index in students.

Analytical procedures for the detection of certain local anesthetics (including lidocaine, trimecaine, articaine, and anilocaine) in blood and urine are described with special reference to their application in chemotoxicological studies. The description is focused on the methods for isolation of the assay preparations from biological fluids and their identification with the help of up-to-date chromatographic techniques, such as thin layer chromatography, high performance liquid chromatography, and gas-liquid chromatography.

The purpose of the present study was to determine whether there is a menstrual cycle effect on heart rate, blood pressure and heart rate variability. 10 healthy regularly cycling females (age 19-23 years) were studied during the follicular phase and luteal phase over two month. We found significant changes in heart rate, AMo and stress index during the menstrual cycle with a minimum in the follicular phase and maximum in the luteal phase. The HF and LF components decreased more during the luteal phase than during the follicular phase (p < 0.05), whereas a tendency for increase LF/HF was observed in the luteal phase. In the follicular phase SDNN, pNN50, Mo, MxDMn were significantly higher than in the luteal phase. Furthermore, the VIK was higher in the luteal phase compared to the follicular phase (p = 0.003). Blood pressure did not show any significant change during both these phases of the menstrual cycle. These findings indicate that sympathetic nervous activity in the luteal phase is greater than in the follicular phase, whereas parasympathetic nervous activity is predominant in the follicular phase. A difference of the balance of ovarian hormones may be responsible for these changes of autonomic functions during the menstrual cycle.

Growth characteristics of Hormoconis resinae (Lindau) von Arx & de Vries, isolated from premises of the 4th unit of the Chernobyl NPP were studied on solid and liquid media. Radial growth rate of the studied strains varied from 0.05 to 0.08 mm an hour and did not depend on radioactivity of isolation place and composition of the nutrient medium. Index of substrate I colonizing intensity has demonstrated that all studied strains grew better on nutrient-rich medium. Morphological changes in studied strains on starving nutrient-poor media showed a decrease of biomass accumulation, reduction of sporogenesis, inhibition of micelial growth. Concerning both characteristic of biomass accumulation and morphological changes, all studied strains were referred to 3 groups: (1) heterotrophic strains, (2) strains with a tendency to oligocarbotolerance, (3) strains of intermediate group.

The effect of hydrogen peroxide ( 10(-9)-10(-1) M) on the mycelial growth of the fungi Alternaria alternata, Cladosporium cladosporioides, Mucor hiemalis, and Paecilomyces lilacinus has been studied. The growth of fungi isolated from habitats with a background level of radioactive contamination was stopped by H2O2 concentrations equal to 10(-3) and 10(-2) M, whereas the growth of fungi that were isolated from habitats with high levels of radioactive contamination was only arrested by 10(-1) M H2O2. The response of the different fungi to hydrogen peroxide was of three types: (1) a constant growth rate of fungal hyphae at H2O2 concentrations between 10(-9) and 10(-4) M and a decrease in this rate at 10(-3) M H2O2, (2) a gradual decrease in the growth rate as the H2O2 concentration was increased, and (3) an increase in the growth rate as the H2O2 concentration was increased from 10(-7) to 10(2)-5 M. The melanin-containing species A. alternata and C. cladosporioides exhibited all three types of growth response to hydrogen peroxide, whereas the light-pigmented species M. hiemalis and P. lilacinus showed only the first type of growth response. A concentration of hydrogen peroxide equal to 10(-1) M was found to be lethal to all of the fungi studied. The most resistant to hydrogen peroxide was found to be the strain A. alternata 56, isolated from the exclusion zone of the Chernobyl Nuclear Power Plant.

The results of surgical treatment of 600 patients with rectal fistula were analyzed. Intrasphincter fistula was observed in 204 patients (34%), transsphinctered fistula--in 72 patients (12%), extrasphincter fistula--in 324 patients (54%). In order to decrease the rate of recurrence of fistula, during performance of plastics of internal hole of the fistula the graft of all the wall of rectum (not only its mucosa) was mobilized up to the level of anal sphincter. Post-operative complications and recurrence of fistula occurred in 4% of patients.

The experience of treatment of 9 patients with metachronous megacolon and colonic cancer was analyzed. Clinical signs of the disease are diverse. Radical surgical intervention is singularis method of treatment of such patients.