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In Korea, ship collisions represent the majority of marine accidents and are substantially more likely in port areas where ship traffic is dense. To improve the safe navigation of vessels, the Korea Maritime Safety Audit (KMSA) has been conducting maritime traffic flow evaluations based on the Environmental Stress (ES) model since 2009. The ES model quantifies the subjective stress felt by the navigator; however, it does not reflect the relative bearing between ships. Risk assessment models using the ship domain are designed to consider the conditions of a potential encounter with a target ship and the possibility of avoidance action. However, even if the target ship does not invade the domain of the own ship, collision may occur at the closest point of approach (CPA). Therefore, in this study, the Collision RIsk (CoRI) model which moves the existing ship domain to the CPA is applied to compare the collision risk according to the navigator's awareness of the vessel encounter conditions. The results demonstrate that the CoRI model is a better risk assessment method than the ES model because it sensitively reflects the risks due to collision avoidance action, such as course alteration and speed changes.

This work studied Gram-positive and weak electricigen Corynebacterium glutamicum for its ability to transfer electrons and to produce bioelectricity in microbial fuel cells (MFCs). The electrochemical and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) results revealed that C. glutamicum had the potential to mediate electron transfer to an electrode by emitting its own extracellular electron shuttles such as flavins. To enhance the current collection from C. glutamicum, a carbon cloth anode was modified with ferrocene-branched chitosan hydrogel (redox-hydrogel). The maximum current density of the ferrocene-branched chitosan redox hydrogel anode with C. glutamicum was drastically increased to 120 &micro, A cm&minus, 2 relative to a bare carbon cloth electrode with C. glutamicum (261 nA cm&minus, 2). The power density and polarization curves for the MFC operation with the redox-hydrogel-modified anode showed that C. glutamicum effectively generated bioelectricity by means of the redox-hydrogel anode. The results suggest that, in such an electro-fermentation process, ferrocene-branched chitosan hydrogel grafted onto an anode surface would also facilitate both electron transfer from C. glutamicum to the anode and bioelectricity generation.

The MAPK signaling cascade is universal among eukaryotes and mediates a variety of environmental and developmental responses. Two Arabidopsis MAPKs, MPK3 and MPK6, have been shown to be activated by various stimuli and suggested as a convergence point of different signaling pathways. It is known that these MAPKs, MPK3/MPK6, control the discrete stages of stomatal development in Arabidopsis, but how they are regulated and how the same MAPK components can achieve signaling specificity is largely unknown. We recently demonstrated that MAP Kinase Phosphatase 1 (MKP1) promotes stomatal differentiation by suppressing activation of MPK3/MPK6 in the stomatal lineage. By expressing MKP1 in discrete stomatal precursor cell types, we further identified that MKP1 plays an important role at the early stage of stomatal development for the cell fate transition leading to stomatal differentiation. While MKP1 was previously known as a key regulator of environmental stress responses, our data illustrate a novel role of MKP1 in plant development: it acts as one of the specificity-determining regulators of MAPK signaling to enforce proper stomatal development in Arabidopsis.

In this study, lipid extraction from Chlorella vulgaris was performed by using mixtures of molten salt and ionic liquid. The yield-enhancing effects of blending of molten salt with ionic liquid were investigated. Among the three molten salts (Zn(NO 3 ) 2 ·6H 2 O, Mg(ClO 4 ) 2 ·6H 2 O, and FeCl 3 ·6H 2 O), FeCl 3 ·6H 2 O showed a high lipid extraction yield (113.0 mg/g cell) and good reaction performance. When FeCl 3 ·6H 2 O was mixed with [Emim]OAc (5:1, w/w), the lipid extraction yield increased to 227.6 mg/g cell, a performance similar to that of single [Emim]OAc (218.7 mg/g cell). When lipid was extracted by the FeCl 3 ·6H 2 O/[Emim]OAc mixture at a 5:1 (w/w) blending ratio, 90 °C temperature, and 1 h duration, the fatty acid content of the extracted lipid was 981.7 mg/g lipid, indicating less than 2% impurity. The lipid extraction from C . vulgaris was improved by the synergistic effects of molten salt and ionic liquid with different ions.

Consumption of waxy corn is steadily increasing due to consumer preference for natural food. However, availability of waxy corn is limited because most of them are used for steamed waxy corn. It is therefore important to create new demands of waxy corn using food processing technology. There is little prior research about characteristics of milled kernel in waxy corn. This study examined the physicochemical and cooking properties of milled kernel using nine waxy corn hybrids. Colored hybrids (Heukjinju, Miheukchal, and Eolrukchal1) showed high milling yield and low change of grain length after milling process compared to other hybrids. The total starch content ranged from 62.1 to 68.4% and the gelatinization temperature of starch was lowest in Yeonnong1 (69.9℃). The breakdown viscosity of Yeonnong1, Miheukchal and Heukjinju was higher than those of tested hybrids, whereas setback viscosity of those hybrids was lower. In cooking properties of milled waxy corn, water absorption and volume expansion rate were high in Yeonnong2 (84.9, 219.3%), Miheukchal (85.9, 211.4%), and Heukjinju (80.9, 203.7%). In the sensory test, the overall preference was significantly higher in Heukjinju and Miheukchal. Thus, Heukjinju, Yeonnong2, and Miheukchal showed good scores in milling yield, cooking properties, and the preference of sensory test. These results will provide fundamental information to extend milled waxy corns usage.Keywords : corn, milling corn, physicochemical, cooking quality

Magnesia-zirconia (MgO-ZrO2) support was prepared by a sol-gel method, and magnesium vanadate nano-catalysts supported on magnesia-zirconia (X-Mg3(VO4)2/MgO-ZrO2) were then prepared by a wet impregnation method with a variation of vanadium content (X = 6.6, 9.9, 12.8, 15.2, and 19.1 wt%). X-Mg3(VO4)2/MgO-ZrO2 nano-catalysts were applied to the oxidative dehydrogenation of n-butane to n-butene and 1,3-butadiene. The formation of X-Mg3(VO4)2/MgO-ZrO2 nano-catalysts was well confirmed by XRD, XPS, and ICP-AES analyses. 15.2-Mg3(VO4)2/MgO-ZrO2 and 19.1-Mg3(VO4)2/MgO-ZrO2 catalysts experienced a catalyst deactivation, while the other Mg3(VO4)2/MgO-ZrO2 catalysts showed a stable catalytic performance during the whole reaction time. The effect of oxygen property of X-Mg3(VO4)2/MgO-ZrO2 nano-catalysts on the catalytic performance in the oxidative dehydrogenation of n-butane was investigated. Experimental results revealed that oxygen capacity of the catalyst was closely related to the catalytic performance, while oxygen mobility of the catalyst played an important role in the catalyst stability. Among the catalysts tested, 12.8-Mg3(VO4)2/MgO-ZrO2 catalyst showed the best catalytic performance in terms of yield for TDP (total dehydrogenation products).

Repetitive transcranial magnetic stimulation (rTMS) is used to treat neurological diseases such as stroke and Parkinson's disease (PD). Although rTMS has been used clinically, its underlying therapeutic mechanism remains unclear. The objective of the present study was to clarify the neuroprotective effect and therapeutic mechanism of rTMS in an animal model of PD. Adult Sprague-Dawley rats were unilaterally injected with 6-hydroxydopamine (6-OHDA) into the right striatum. Rats with PD were then treated with rTMS (circular coil, 10 Hz, 20 min/day) daily for 4 weeks. Behavioral assessments such as amphetamine-induced rotational test and treadmill locomotion test were performed, and the dopaminergic (DA) neurons of substantia nigra pas compacta (SNc) and striatum were histologically examined. Expression of neurotrophic/growth factors was also investigated by multiplex ELISA, western blotting analysis and immunohistochemistry 4 weeks after rTMS application. Among the results, the number of amphetamine-induced rotations was significantly lower in the rTMS group than in the control group at 4 weeks post-treatment. Treadmill locomotion was also significantly improved in the rTMS-treated rats. Tyrosine hydroxylase-positive DA neurons and DA fibers in rTMS group rats were greater than those in untreated group in both ipsilateral SNc and striatum, respectively. The expression levels of brain-derived neurotrophic factor, glial cell line-derived neurotrophic factor, platelet-derived growth factor, and vascular endothelial growth factor were elevated in both the 6-OHDA-injected hemisphere and the SNc of the rTMS-treated rats. In conclusion, rTMS treatment improved motor functions and survival of DA neurons, suggesting that the neuroprotective effect of rTMS treatment might be induced by upregulation of neurotrophic/growth factors in the PD animal model.

The purpose of this study was to investigate the effects of children's temperament, parent-child communication, peer relationships on children's cognitive and affective happiness. The participants consisted of 396 elementary school students aged 10-13 dwelling in Jeonbuk province. Data was analyzed through descriptive statistics, t-test, regression analysis using the SPSS 18.0 program. The most salient results from this study were as follows: first, there were statistically significant differences in the children's happiness according to academic performance and family's economic level; children who considered themselves to have good grades and to have a higher standard of living were happier than other groups. However, there was no significant difference in children's happiness in relation to gender. Second, children's temperament(sociability, activity), parent-child open communication and peer relationships were positively associated with the children's cognitive and affective happiness. Children's emotional temperament, parent-child problem communication were negatively associated with the children's cognitive and affective happiness. Third, as the results of regression analysis, parent-child open communication, children's temperament(activity, sociability), peer relationships were considered to be the most influential factors in explaining cognitive happiness. And parent-child open communication, parent-child problem communication, children's temperament(activity) were influential factors in explaining affective happiness. In conclusion, children's happiness is critically affected not only by internal variables but also environmental ones such as parents and peers.

To produce biodiesel efficiently from fish oil containing 4% free fatty acid, esterification and trans-esterification were carried out with Vietnam catfish oil, which was kindly provided from GS-bio company. Heterogeneous solid acid catalysts such as Amberlyst-15 and Amberlyst BD-20 and sulfuric acid as homogeneous acid catalyst were used for the esterification of free fatty acids in the fish oil. Sulfuric acid showed the highest removal efficiency of free fatty acid and the shortest reaction time among three acid catalysts. The base catalysts for trans-esterification such as KOH, NaOCH3 and NaOH were compared with each other and KOH was determined to be the best transesterification catalyst. Some solid material, which assumed to be sapo- nified product from glycerol and biodiesel, were observed to form in the fish oil biodiesel when using NaOCH3 and NaOH as the transesterification catalyst. The initial acid value of fish oil was proven to have a negative effect on biodiesel conversion. Of the three catalysts, KOH catalyst transesterification was shown to have high content of FAME and the optimal ratio of methanol/oil ratio was identified to be 9:1.

A study using 3D virtual garment simulation is carried out for the evaluation and application on the pants fit for obese women in their age of 20s and 30s. The results are as follows; 5 obese women in their 20s and 30s were selected for the testing 3D body. They showed no significant differences in all items, comparing with the data of 5th Size Korea body dimensions. The average waist circumstance of the subjects` 3D body dimensions was 87.0cm, hip circumstance was 102.4cm, BMI was , and their obese body types had similar mean values. Based on the detailed design of ready made pants and the study results of 20~30s obese women preference for pants design, pants of straight silhouette and semi-tight fit which have waist line lowered, no front dart and one back dart, were manufactured with 100% black cotton and cotton spandex mixed fabrics. When comparing the appearances between real garment and virtual garment, the average of the real garment with 100% cotton was 3.70 and the virtual garment was 4.05. The average of real garment with cotton spandex mixed fabrics was 3.75 and the virtual garment was 4.06. Therefore, the average of virtual garment was highly evaluated. When comparing the results of evaluating the appearance, there was no significant difference caused by materials between real garment and virtual garment. The expression for the ease of virtual garment and real garment was also similar for good evaluation. Thus, 3D virtual garment simulation did positively prove its reliability and effect.

The nursery active-wear uniform designs of Internet shopping malls are simple and similar; however, the lat- est children's wear trends (mostly composed of training jumpers and pants) do not reflect these qualities. Children's folk- lore plays (widely used in child education) have an instructive value for the emotional and intellectual development of a child. Nursery active-wear uniform designs applied to children's folklore's plays could be positive for the emotional devel- opment and specialized cultural education of children. We designed three nursery active-wear uniforms with three dif- ferent design concepts (casual, semi-formal and sporty) regardless of sex. The main color of the three nursery active-wear uniforms was yellow. We used three different chroma and values of yellow through three design concepts: Design I of a nursery active-wear uniform (a training suit style with a good activity), Design II of a nursery active-wear uniform (a semi suit style), and Design III of a nursery active-wear uniform (a sportswear style that reflected a sport wear trend). We expressed children's folklore plays in the pattern of a nursery active-wear uniform. The pattern was characterized as a pair of bears flying kites. This pattern was utilized in three nursery active-wear uniforms with the logo and name of the nursery. We modified the designs of active-wear nursery uniforms based on an evaluation of 33 special panels. We made three nursery active-wear uniforms for five-year olds.

BACKGROUND Since extrusion has many desirable characteristics such as high shear, rapid mixing, short residence time, controllable barrel temperature, and adaptability to process modification, the technology has been applied as a viable continuous pretreatment method for empty fruit bunches (EFB). RESULTS Optimized conditions for a continuous twin-screw-driven reactor (CTSR) were determined to be 170°C barrel temperature, 1.6% (w/v) sodium hydroxide, 1:8 solid to liquid ratio, and 5 rpm screw rotational speed. Under these operating conditions, 56.55% of lignin and 39.18% of hemicellulose fractions were solubilized from raw EFB, which increases the glucan content in pretreated EFB from 39.25% to 53.60%. After subsequent 48-h enzymatic hydrolysis of pretreated EFB, the highest digestibility of 92.4% was achieved with an enzyme loading of 30 FPU g−1-glucan for cellulase and 70 pNPG g−1-glucan for β-glucosidase. CONCLUSION CTSR processing has great potential for increasing the efficiency of EFB pretreatment compared with batch pretreatments. © 2013 Society of Chemical Industry

Microalgae biorefinement has attracted in intensive academic and industrial interest worldwide for its potential to replace petrol biofuels as economically and environmentally advantageous alternatives. However, harvesting and lipid extraction remain as critical and difficult issues to be resolved. In the present study, four amino-groups functionalized organic-nano clays were prepared. Specifically, Mg or Al or Ca backboned and covalently linked with 3-aminopropyltriethoxysilane or 3-[2-(2-aminoethylamino)ethylamino]propyltrimethoxysilane by sol-gel reaction under ambient conditions, resulted in Mg-APTES clay, Al-APTES clay, Ca-APTES clay, and Mg-N3 clay, respectively. Each organic-nanoclay was utilized for lipid extraction from wet microalgae biomass. As a result, the lipid-extraction efficiency of paste docosahexaenoic acid (DHA)-rich Chlorella sp. with low lipid content was high, while one of paste oleaginous Chlorella sp. with high lipid content was relatively low. Despite the low lipid-extraction efficiencies in all of the wet microalgae biomass, the conversion of the extracted lipids' fatty acids to biodiesel was nearly 100%.

Receptor kinases play a central role in various biological processes, but due to their low abundance and highly hydrophobic and dynamic nature, only a few of them have been functionally characterized, and their partners and ligands remain unidentified. Receptor protein extraction and purification from plant tissues is one of the most challenging steps for the success of various biochemical analyses to characterize their function. Immunoprecipitation is a widely used and selective method for enriching or purifying a specific protein. Here we describe two different optimized protein purification protocols, batch and on-chip immunoprecipitation, which efficiently isolate plant membrane receptor kinases for functional analysis.

Background: Current single-stage delignification-pretreatment technologies to overcome lignocellulosic biomass recalcitrance are usually achieved at the expense of compromising the recovery of the polysaccharide components, particularly the hemicellulose fraction. One way to enhance overall sugar recovery is to tailor an efficient two-stage pretreatment that can pre-extract the more labile hemicellulose component before subjecting the cellulose-rich residual material to a second-stage delignification process. Previous work had shown that a mild steam pretreatment could recover >65% of the hemicellulose from poplar while limiting the acid-catalysed condensation of lignin. This potentially allowed for subsequent lignin extraction using various lignin solvents to produce a more accessible cellulosic substrate. Results: A two-stage approach using steam and/or solvent pretreatment was assessed for its ability to separate hemicellulose and lignin from poplar wood chips while providing a cellulose-rich fraction that could be readily hydrolysed by cellulase enzymes. An initial steam-pretreatment stage was performed over a range of temperatures (160–200 °C) using an equivalent severity factor of 3.6. A higher steam temperature of 190 °C applied over a shorter residence time of 10 min effectively solubilized and recovered 75% of the hemicellulose while enhancing the ability of various solvents [deep eutectic solvent (DES), ethanol organosolv, soda/anthraquinone (soda/AQ) or a hydrotrope] to extract lignin in a second stage. When the second-stage treatments were compared, the mild DES treatment (lactic acid and betaine) at 130 °C, removed comparable amounts of lignin with higher selectivity than did the soda/AQ and organosolv pretreatments at 170 °C. However, the cellulose-rich substrates obtained after the second-stage organosolv and soda/AQ pretreatments showed the highest cellulose accessibility, as measured by the Simon’s staining technique. They were also the most susceptible to subsequent enzymatic hydrolysis. Conclusions: The second-stage pretreatments varied in their ability to solubilize and extract the lignin component of steam-pretreated poplar while enhancing the enzymatic hydrolysis of the resulting cellulose-rich residual fractions. Although DES extraction was more selective in extracting lignin from the steam-pretreated substrates, the organosolv and soda/AQ post treatments disrupted the cellulose structure to a greater extent while enhancing the ease of enzymatic hydrolysis. Graphical abstract Effective hemicellulose removal via steam pretreatment followed by subsequent lignin extraction under acidic, alkaline or solvolytic conditions results in a highly accessible, more readily hydrolysed cellulose fraction.

Peptide ligand–receptor kinase interactions have emerged as a key component of plant growth and development. Now, highly related small signaling peptides have been shown to act antagonistically on the same receptor kinase, providing new insights into how plants optimize developmental processes using competitive peptides.

Secreted peptides mediate intercellular communication [1 and 2]. Several secreted peptides in the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family regulate morphogenesis of tissues, such as stomata and inflorescences in plants [3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15]. The biological functions of other EPFL family members remain unknown. Here, we show that the EPFL2 gene is required for growth of leaf teeth. EPFL2 peptide physically interacts with ERECTA (ER) family receptor-kinases and, accordingly, the attenuation of ER family activities leads to formation of toothless leaves. During the tooth growth process, responses to the phytohormone auxin are maintained at tips of the teeth to promote their growth [ 16, 17, 18 and 19]. In the growing tooth tip of epfl2 and multiple er family mutants, the auxin response becomes broader. Conversely, overexpression of EPFL2 diminishes the auxin response, indicating that the EPFL2 signal restricts the auxin response to the tooth tip. Interestingly, the tip-specific auxin response in turn organizes characteristic expression patterns of ER family and EPFL2 by enhancing ER family expression at the tip while eliminating the EPFL2 expression from the tip. Our findings identify the novel ligand-receptor pairs promoting the tooth growth, and further reveal a feedback circuit between the peptide-receptor system and auxin response as a mechanism for maintaining proper auxin maxima during leaf margin morphogenesis.

The purpose of this study was to investigate the effects of body weight, perception of body shape and satisfaction with body shape on obesity stress and self-esteem among middle school students. The participants in this study were 446 middle school students in K-city. The major results of this study are as follows. First, male students demonstrated higher obesity rates than female students, and female students considered themselves to be fatter and less satisfied with their body shape than male students. Female students had significantly more obesity-related stress than male students. Second, there were significant differences in physical obesity stress according to body weight status among male and female students, and there was statistically significant difference in psychological obesity stress according to body weight status among male students. Male and female students who considered themselves to be fatter and less satisfied with their body shape suffered from high obesity stress. Third, there was no significant difference in self-esteem according to body weight status, but self-esteem was affected by perception and satisfaction of body shape; male and female students who considered themselves to be fatter and less satisfied with their body shape showed low levels of self-esteem. As the result of regression analysis, psychological obesity stress was the strongest factor in explaining self-esteem among all students, and satisfaction of body shape was also found to be a significant variable among female students.

In this study, 4-methylmorpholine N-oxide (NMMO) and four ionic liquids were compared to determine the most suitable type for use in pretreatment of oil palm empty fruit bunch (EFB) to enhance enzymatic digestibility and obtain maximal fermentable sugar (glucose). Four types of ionic liquids, namely, 1-ethyl-3-methyl imidazolium acetate ([Emim]OAc), 1-butyl-3-methyl imidazolium chloride ([Bmim]Cl), tributyl methyl ammonium methylsulfate (MTBS), and 1-ethyl-3-methyl imidazolium diethylphosphate ([Emim]DEP), were used. The effects of pretreatment temperature and time on the enzymatic digestibility of EFB were examined. Among NMMO and four ionic liquids, NMMO and [Emim]OAc, showing high enzymatic digestibility, were found to be most effective for EFB pretreatment. The enzymatic digestibility of NMMO-pretreated EFB increased as pretreatment temperature and time increased. After [Emim]OAc pretreatment, the crystallinity index of EFB decreased, due to the conversion of crystalline cellulose to amorphous cellulose, and the enzymatic digestibility thereby was increased to 96.6% at 110 °C for 2 h.

Gout occurs as a response to monosodium urate crystal, that is present in joints, bones and soft tissue. The classic symptoms of gouty arthritis are recurrent attacks of acute, markedly painful monoarticular or oligoarticular inflammation; but polyarthritis and chronic arthritis can also occur. Differential diagnosis from infectious arthritis is important. A definitive diagnosis requires the direct identification of urate crystals in the joint, and the exclusion of infection. We report the case of systemic inflammatory response syndrome (SIRS), developed from acute polyarticular gout. SIRS is characterized by loss of local control of inflammation, or an overly activated response resulting in an exaggerated systemic response. The SIRS was presumably due to systemic effects of a localized inflammatory response to urate crystals.

X-Mg3(VO4)2/MgO–ZrO2 catalysts were prepared by a wet impregnation method with a variation of vanadium content (X = 2.8, 4.2, 5.6, 9.0, and 11.2 wt%), and they were applied to the oxidative dehydrogenation of n-butane. Effect of oxygen capacity and acidity of Mg3(VO4)2/MgO–ZrO2 catalysts on the catalytic performance in the oxidative dehydrogenation of n-butane was investigated. Experimental results revealed that oxygen capacity and acidity of catalyst were closely related to the catalytic performance in the oxidative dehydrogenation of n-butane. It was found that the catalytic performance of X-Mg3(VO4)2/MgO–ZrO2 catalysts increased with increasing oxygen capacity and with increasing acidity of the catalyst.

Eight-zone para-xylene (PX) simulated moving-bed (SMB) involves three flushing sequences to prevent purity deterioration caused by SMB bed-lines connecting a rotary valve to beds. Beside the recycle flow from the last zone to the first, the extract fluid trapped in bed-line (BL) is fed back to one bed ahead of the extract bed in accord with the line flushing ( LF ) and secondary flushing ( SF i ), forming an internal recycle flow. Both LF and SF i form the closed loop to increase purity and recovery, while the tertiary flushing ( TF o ) works somewhat independently of the other flushing streams for recovery enhancement. Thus, a two-level optimization strategy was proposed to identify optimum bed-configuration and flushing flow rates of LF , SF i and TF o . This study also examined how axial dispersion inside BL influences recovery and purity. The paper shows that the annual profit of the 8-zone SMB increases by 50 tons of PX/year over that of 7-zone SMB.

Vanadium-magnesium oxide catalysts supported on nano-structured MgO and ZrO2 (Mg3(VO4)2/MgO/ZrO2) were prepared by a wet impregnation method with a variation of Mg:Zr ratio (8:1, 4:1, 2:1, and 1:1). For comparison, Mg3(VO4)2/MgO and Mg3(VO4)2/ZrO2 catalysts were also prepared by a wet impregnation method. The prepared catalysts were applied to the oxidative dehydrogenation of n-butane in a continuous flow fixed-bed reactor. Mg3(VO4)2/MgO/ZrO2 (Mg:Zr = 4:1, 2:1, and 1:1) and Mg3(VO4)2/ZrO2 catalysts showed a stable catalytic activity during the whole reaction time, while Mg3(VO4)2/MgO/ZrO2 (8:1) and Mg3(VO4)2/MgO catalysts experienced a severe catalyst deactivation. Deactivation of Mg3(VO4)2/MgO/ZrO2 (8:1) and Mg3(VO4)2/MgO catalysts was due to their low oxygen mobility. Effect of oxygen capacity (the amount of oxygen in the catalyst involved in the reaction) of the supported Mg3(V04)2 catalysts on the catalytic performance in the oxidative dehydrogenation of n-butane was investigated. Experimental results revealed that oxygen capacity of the catalyst was closely related to the catalytic activity in the oxidative dehydrogenation of n-butane. A large oxygen capacity of the catalyst was favorable for obtaining a high catalytic activity in this reaction. Among the catalysts tested, Mg3(VO4)2/MgO/ZrO2 (4:1) catalyst with the largest oxygen capacity showed the best catalytic performance.

Crude canola oil (CCO) contains about 100–300 ppm of phospholipids, which have shown negative effects on biodiesel/buffer solution phase separation, resulting in low biodiesel production yield. Therefore, phospholipids should be removed before transesterification by a degumming process for efficient production of biodiesel. In this study, two-step enzymatic processes (degumming and transesterification) were carried out for the production of biodiesel from CCO. Degumming of CCO was performed using phospholipase A2 as a degumming reagent. The initial phospholipid content was reduced to less than 5 ppm by enzymatic degumming. The effects of three formulations of enzyme catalyst on the efficiency of transesterification were investigated. As a result, conversion rates of degummed CCO to fatty acid methyl esters (FAME) were 68.56%, 70.15%, and 84.25%, respectively. Lipase formulation composed of a 1:1 (vol:vol) enzyme mixture of Rhizopus oryzae and Candida rugosa showed the best performance among those tested. In order to recover and reuse the lipase catalyst efficiently, a 1:1 enzyme mixture of R. oryzae and C. rugosa was immobilized on silica gel. The immobilized lipase was used in subsequent transesterification optimization experiments. Optimization of transesterification was performed by response surface methodology (RSM). A total of 20 experiments based on RSM were carried out, and the optimal reaction conditions appeared to be 24.4% (w/w) immobilized catalyst, 13.5% (w/w) buffer solution, and 15.8% (w/w) methanol based on oil mass. Conversion rate of degummed CCO to FAME was determined to be 88.9% under optimal conditions.

MgO–ZrO 2 supports with different Mg:Zr ratios (16:1, 8:1, 4:1, 2:1, and 1:1) were prepared by a gel-oxalate co-precipitation method, and Mg 3 (VO 4 ) 2 /MgO–ZrO 2 catalysts were then prepared by a wet impregnation method. The prepared catalysts were applied to the oxidative dehydrogenation of n-butane to n-butene and 1,3-butadiene. Experimental results revealed that oxygen capacity and acidity of the catalyst were closely related to the catalytic performance. Among the catalyst tested, Mg 3 (VO 4 ) 2 /MgO–ZrO 2 (4:1) catalyst, which retained the largest oxygen capacity and acidity, exhibited the best catalytic performance in the oxidative dehydrogenation of n-butane.

Multicellular organisms achieve final body shape and size by coordinating cell proliferation, expansion, and differentiation. Loss of function in the Arabidopsis ERECTA ( ER ) receptor-kinase gene confers characteristic compact inflorescence architecture, but its underlying signaling pathways remain unknown. Here we report that the expression of ER in the phloem is sufficient to rescue compact er inflorescences. We further identified two EPIDERMAL PATTERNING FACTOR-LIKE ( EPFL ) secreted peptide genes, EPFL4 and EPFL6/CHALLAH ( CHAL ), as redundant, upstream components of ER -mediated inflorescence growth. The expression of EPFL4 or EPFL6 in the endodermis, a layer adjacent to phloem, is sufficient to rescue the er -like inflorescence of epfl4 epfl6 plants. EPFL4 and EPFL6 physically associate with ER in planta. Finally, transcriptome analysis of er and epfl4 epfl6 revealed a potential downstream component as well as a role for plant hormones in EPFL4/6- and ER-mediated inflorescence growth. Our results suggest that intercell layer communication between the endodermis and phloem mediated by peptide ligands and a receptor kinase coordinates proper inflorescence architecture in Arabidopsis .

The levels of interleukin-6 (IL-6) are proportionate to injury; it is the most commonly used quantitative marker in surgical studies. Cytokines and the acute-phase response play an important role in controlling the human immune system. The objective of this study was to compare the systemic acute cytokine response and clinical outcomes of conventional laparoscopic and single port laparoscopic cholecystectomy. We compared patients who underwent single port laparoscopic cholecystectomy (the single port group) with patients who underwent conventional laparoscopic cholecystectomy (the conventional group) according to the clinical variables, IL-6, leukocyte subpopulations, and visual analog scale (VAS) pain score. The mean age in the single port group was significantly younger ( P = 0.010) and the mean operation time in the conventional group was significantly shorter ( P = 0.002). Postoperative 4-hour VAS pain score was slightly worse in the single port laparoscopic cholecystectomy group, but was not significantly different. We found no difference in clinical outcomes, the level of serum IL-6, C-reactive protein, leukocyte subpopulations, and complications between the two groups. Stress response in single port laparoscopic cholecystectomy is equal to conventional surgery. Postoperative 4-hour VAS pain score was slightly worse and the operation time is significantly longer in the single port laparoscopic cholecystectomy group.

In this study, the production of bio-diesel from animal oil by esterification and trans-esterification was investigated. There were three different extraction methods for oil extraction from raw animal fat. Heterogeneous catalysts such as Amberlyst-15 and Amberlyst BD-20 and a homogeneous catalyst such as sulfuric acid were used for esterification. Among three catalysts, the removal efficiency of Free Fatty Acid (FFA) was the highest in sulfuric acid. Response surface method was carried out to find the optimal esterification condition of sulfuric acid and methanol. After the esterification under the optimal condition, this animal fat was used for the trans-esterification. Animal oil used for trans-esterification was below 1% of FFA content and 0.09% of water content. The catalysts for trans-esterification were KOH, NaOH and . To investigate the effects of catalyst type and amount on trans-esterification, The amount of catalyst were changed with 0.3, 0.6 and 0.9 wt%. The molar ratio of methanol/oil was changed with 4, 6, 9 and 12. The amount of catalyst was fixed to 0.8 wt%. The KOH catalyst showed the highest FAME conversion for trans- esterification, and the optimal methanol/oil weight ratio was 6. In the experiments of various catalysts and methanol molar ratios, the highest content of FAME is 96%. However, this FAME content was below Korean bio-diesel standard which is 96.5% of FAME content. After distillation, FAME content increased to 98%.

Vanadium-magnesium oxide (Mg3(VO4)2) catalysts supported on Al2O3, ZrO2, MgO, and CeO2 were prepared by a wet impregnation method, and they were applied to the oxidative dehydrogenation of n-butane. Effect of oxygen capacity and oxygen mobility of supported Mg3(VO4)2 catalysts on the catalytic performance in the oxidative dehydrogenation of n-butane was investigated. Experimental results revealed that large oxygen capacity of the catalyst was favorable for obtaining high catalytic activity at the initial stage of reaction, while facile oxygen mobility of the catalyst led to stable catalytic activity during the catalytic reaction in the oxidative dehydrogenation of n-butane.

The present study investigated the mediating effects of negative automatic thoughts on the relationship between the communication styles of fathers and their children`s happiness. The participants consisted of 509 elementary school (225 5th, 6th graders) and middle school (284 1st, 2nd graders) students in Seoul and Jeonju city. The instruments used were the Parent-Adolescent Communication Inventory (Barnes & Olson, 1982), the Korean Children`s Automatic Thoughts Scales (Moon, 2002), and the Happiness Inventory for Children (Lee, 2005). Data were analyzed by means of both descriptive statistics and regression analysis using the SPSS (12.0 version). The results indicated that the effects of a father`s communication style on children`s happiness was partially mediated by their negative automatic thoughts. In conclusion, negative automatic thoughts has a mediating effect upon a father`s communication style as it relates to the happiness of their children.

Solid pseudopapillary neoplasm (SPN) of the pancreas is a rare neoplasm that accounts for about 1 to 2 per cent of all pancreatic tumors. The aim of this study was to delineate the clinicopathological characteristics and surgical outcomes of solid pseudopapillary neoplasm of the pancreas. We retrospectively reviewed the clinicopathological characteristics and surgical outcomes of 18 patients who underwent surgery for SPN of the pancreas between January 1, 2001 and November 1, 2010. The patient group was comprised of 14 females and four males and the median patient age at diagnosis was 32.4 years (range 10∼68 years). Eleven of the 18 patients were symptomatic at the time of diagnosis. The type of surgery was selected according to the location and presentation of the tumor. The resection margins were negative in all patients. One patient had distant metastasis and recurrent mass node repetitively. She underwent seven operations for recurrence of SPN during the follow-up period of 218 months. Complete surgical excision is the treatment of choice for SPN of the pancreas and can give a good prognosis. Although sometimes patients have repetitive metastases or recurrences, patients undergoing complete surgical excision of the tumor will have a good outcome.

Background Patients with umbilical hernias complicated by liver cirrhosis have an increased likelihood of complications following herniorrhaphy. The aim of this study was to investigate the clinical outcomes in patients with umbilical hernias complicated by liver cirrhosis. Methods Between 2001 and 2010, 44 patients were enrolled in this study. The comparison between non-operative and operative group was performed. Patients who underwent emergency versus elective surgery were also compared. Results Of the 44 patients, there were 33 men and 11 women. Thirty-one patients (70.5%) underwent surgery and 13 patients (29.5%) were treated conservatively. Overall morbidity and mortality rates following herniorrhaphy were 42% and 6.5%. The mean albumin level was significantly lower and total bilirubin, creatinine and mean model of end-stage liver disease score were significantly higher in non-operative group than in operative group. Combined resection was performed more frequently in the emergency group than in elective group. A significantly higher proportion of patients in emergency operation group had postoperative complications ( P = 0.01), especially ascites ( P = 0.02). The operative time and postoperative hospital stay were significantly shorter in the elective operation group than in emergency operation group. Conclusions Early, elective repair of umbilical hernias in cirrhotic patients should be advocated considering the hepatic reserve and patient's condition. Ascites control is the mainstay of post-operative management.

The current status of and challenges associated with bioenergy production and utilization in Korea are reviewed in this paper. Bioenergy (particularly transport biofuels) has emerged as a promising option for mitigating Korea's CO2 emissions and enhancing its energy security. Korea's limited biomass resources and the high cost of biofuels are the major barriers to achieving 2030's implementation targets. Efforts to identify new suitable biomass resources for biofuels production are ongoing and intensive. Aquatic biomasses including algae and plantation wastes collected in the Southeast Asia region have been found to have great potential as feedstocks. R&D on technologies that can more efficiently convert biomass materials to biofuels also are underway. It is expected that cost-effective biofuels will be in adequate supply from 2020 and that, by 2030, their use will contribute effectively to the realization of sustainable growth in Korea.

Bile duct stones, which obstruct the common bile duct, potentially result in complications, such as acute cholangitis and pancreatitis. We present a case involving a patient with normal liver function tests from whom we removed a giant common bile duct stone measuring 7.5 centimeters × 4.0 centimeters × 4.0 centimeters. Postoperatively recurrent common bile duct stones were observed and removed with an endoscopic maneuver in the three-year follow-up period. Recurrent bile duct stones are frequently reported in the case of large size of stones or multiple stones. Surgical treatment may then be considered as a first-line treatment in cases of recurrent common bile duct stones. When an endoscopic or surgical approach is used for the treatment of giant common bile duct stones, careful observation is of the utmost importance and treatment innovations may be necessary.

This study was conducted to investigate the combined effect of fatty acid synthase (FASN) and Acetyl CoA Carboxylase-α (ACACA) genes on carcass traits of Korean cattle (Hanwoo). A total of 1,057 commercial Hanwoo cattle provided by the NongHyup Livestock Research Center (NLRC) and Hanwoo Performance Competition (HPC) were used to analyze the effect of four single nucleotide polymorphisms (SNPs) within FASN (g.11280A>G, g.16024A>G, g.16039T>C, and g.17924A>G) and one SNP within ACACA (g.2274G>A) genes. In addition, the effect of genotypic combinations between FASN (g.17924A>G) and ACACA (g.2274G>A) SNPs has been studied with carcass traits. Significant associations were identified between g.17924A>G of FASN and carcass weight and back fat, and between the ACACA gene SNP g.2274G>A and longissimus muscle area with HPC samples. It was also found that both effects of FASN g.17924A>G and ACACA g.2274G>A polymorphisms were consistent in NLRC samples. Combined analyses of both NLRC and HPC samples also revealed the significant associations between the FASN g.17924A>G and carcass weight and back fat and between the ACACA g.2274G>A and back fat, respectively. The effect of the genotypic combination of g.17924A>G within FASN and g.2274G>A within ACACA genes showed that the combination of both GG genotypes of FASN and ACACA SNPs causes higher carcass weight and marbling score. The results of this study indicate that the two SNP markers within the FASN and ACACA genes can be utilized to select superior Hanwoo cows and calves in commercial Hanwoo farms. (Key words : FASN, ACACA, Hanwoo, Carcass Traits, interaction)

This study is to verify hypotheses about the efficacy and success factors of the IT Mentor project(in which 524 students participated) which is one of the most best practices in Korea. The empirical results found that all the aspects of students, industrial experts, professors, support programs and other infrastructure have certain influences on the industry-academia cooperation. In addition, logit regression model was employed to investigate the efficacy factors of industry and university`s satisfaction to IT mentor system. It showed that industry`s satisfaction depends on students` participation in company`s projects and students` improvements of major competence.

In this study, degumming process was carried out for reducing to less than 10 ppm of phosphorus contents and primary properties of crude canola oil including 0.64 mgKOH/g of acid value, 0.09% of water contents, 0.13% of insoluble impurities, and 40 ppm of phosphorus contents. Efficiency of water degumming and enzymatic degumming was compared for the selection of suitable process obtaining feedstock of biodiesel. Degumming method was deter- mined for preparation of raw material of biodiesel, and reaction conditions were also established. The most effective conditions for water degumming were 2% distilled water (w/w oil), 30 o C of reaction temperature, 900 rpm of agitation speed, and 30 min of reaction time, respectively. In case of enzymatic degumming, optimal conditions were found to be 90 ppm of phospholipase A2 (w/w oil), 50 o C of reaction temperature at pH 5, respectively. When comparing water degumming with enzymatic degumming, efficiency of enzymatic degumming was better than water degumming. How- ever, water degumming method was much more suitable for the production of biodiesel feedstock considering reaction time and process feasibility.

The purpose of this study was improvement of the pants fit for obese women. The results were as follows; 5 obese women in their 20s and 30s were selected for the testing 3D body. They showed no significant differences in all items, comparing with the data of 5th Size Korea body dimensions. The average waist circumstance of the subjects` 3D body dimensions was 87.0 cm and hip circumstance was 102.4 cm, and their obese body types had similar mean values. Based on the survey results and the 3D body dimensions of 5th Size Korea body dimensions, a pants pattern to supplement their body type and improve a fit was designed with waist circumference(88 cm), hip circumference(103 cm), crotch length(27.1 cm), thigh circumference(62 cm), pants length(97 cm), pants hem line circumference(44 cm). Based on the detailed design of ready made pants and the study results of young obese women`s preference for pants design, pants of straight silhouette and semi-tight fit which have waist line lowered by 3.5 cm from the waist circumference line, 3.5 cm belt width, no front dart and one back dart, were manufactured with 100% black cotton and cotton spandex mixed fabrics.

Single port laparoscopic surgery has come to the forefront of minimally invasive surgery. For those familiar with conventional techniques, however, this type of operation demands a different type of eye/hand coordination and involves unfamiliar working instruments. Herein, the authors describe the learning curve and the clinical outcomes of single port laparoscopic cholecystectomy for 150 consecutive patients with benign gallbladder disease. All patients underwent single port laparoscopic cholecystectomy using a homemade glove port by one of five operators with different levels of experiences of laparoscopic surgery. The learning curve for each operator was fitted using the non-linear ordinary least squares method based on a non-linear regression model. Mean operating time was 77.6 ± 28.5 min. Fourteen patients (6.0%) were converted to conventional laparoscopic cholecystectomy. Complications occurred in 15 patients (10.0%), as follows: bile duct injury (n = 2), surgical site infection (n = 8), seroma (n = 2), and wound pain (n = 3). One operator achieved a learning curve plateau at 61.4 min per procedure after 8.5 cases and his time improved by 95.3 min as compared with initial operation time. Younger surgeons showed significant decreases in mean operation time and achieved stable mean operation times. In particular, younger surgeons showed significant decreases in operation times after 20 cases. Experienced laparoscopic surgeons can safely perform single port laparoscopic cholecystectomy using conventional or angled laparoscopic instruments. The present study shows that an operator can overcome the single port laparoscopic cholecystectomy learning curve in about eight cases.

PtRuPd nanoparticles on carbon black were prepared and characterized as electrocatalysts for methanol oxidation reaction in direct methanol fuel cells. Nano-sized Pd (2-4 nm) particles were deposited on Pt/C and PtRu/C (commercial products) by a simple chemical reduction process. The structural and physical information of the PtRuPd/C were confirmed by TEM and XRD, and their electrocatalytic activities were measured by cyclic voltammetry and linear sweep voltammetry. The catalysts containing Pd showed higher electrocatalytic activity for methanol oxidation reaction than the other catalysts. This might be attributed to an increase in the electrochemical surface area of Pt, which is caused by the addition of Pd; this results in increased catalyst utilization.

This investigation examined the association between demographic variables, acculturation and acculturative stress. Participants in the study were 360 Pilipino, Vietnamese, Chinese, Chinese-Korean and Japanese woman who had married internationally and were living in Jeonbuk-Do. Hierarchical regression analyses determined that among the independent variables examined, age, Korean language ability, marital satisfaction, and ethnic identity had significant predictive effects on the levels of acculturative stress experienced. Young women who had lower Korean language proficiency, lower marital satisfaction, and higher ethnic identity showed more higher level of acculturative stress. Implications of this research were discussed with particular attention given to possible support programs for women in international marriages.

This study examined the effect of parents` neglect behavior and children`s irrational beliefs on children`s anger expression style. The participants were 379 5th and 6th grade elementary school children. The major findings were as follows. Children tended to perceive higher levels of their parents` cognitive and emotional neglect than physical and home environmental neglect. Boys perceived higher levels of their parents` physical, supervisory and home environmental neglect than girls, and girls showed higher level of excessively self-expecting belief than boys. There were statistically significant correlations between the parents` neglect behavior, their child`s irrational beliefs and anger expression style. A regression analysis showed that parents` physical and cognitive neglect were predictors of children`s anger control. Children`s excessive apprehension and avoidance beliefs were the best predictors of children`s anger-in, while children`s deterministic belief was the best predictor of children`s anger-out. Parents` physical neglect behavior appeared to be powerful predictor.

This study explored Korean college students' place attachment to current home environments and their place attachment to childhood home environments. The study sought to confirm the relationship between childhood place attachment and collegehood place attachment to home environment. Data were collected from 336 students at four universities in Jellabuk-Do, Korea, and questionnaire survey was performed. Exploratory and confirmatory factor analysis was computed and showed five factors related to place attachment of home environment. Place intimacy, rootedness, place identity, place dependence, and place palyness showed in childhood place attachment to home; place identity, place affection, place restfulness, place dependence, and rootedness in collegehood place attachment to home. Canonical correlation analysis showed that childhood place attachment to home explained 43.9% variance with regard to collegehood place attachment to home. This relationship implied that childhood place attachment strongly affected subsequent collegehood place attachment.

The feasibility of the production of biodiesel from trap grease containing 51.5% free fatty acids (FFAs) was investigated. The esterification of FFAs by an acid catalyst followed by the transesterification of triglycerides by an alkali catalyst was examined. The esterification of trap grease by sulfuric acid as a homogeneous catalyst or by Amberlyst-15 as a heterogeneous catalyst was optimized through a response surface methodology. After the two-step esterification of trap grease by sulfuric acid, the acid value decreased from 102.9 mg KOH/g to 2.75 mg KOH/g. Through the transesterification by potassium hydroxide, fatty acid methyl ester (FAME) content reached 92.4%. Following the esterification of trap grease by Amberlyst-15, the acid value decreased to 3.23 mg KOH/g. With the transesterification by potassium hydroxide, FAME content increased to 94.1%. After the distillation of the produced biodiesel, FAME content increased again, to 97.6%. The oxidation stability of the trap grease biodiesel was 0.17 h, and its cold filter plugging point was 4 °C. As the FAME content of the trap grease biodiesel satisfies the Korean Biodiesel Standard, the trap grease biodiesel seems to be applicable for use as an engine fuel after properties improvement.

Biodiesel is a renewable fuel which can be produced through an esterification reaction. The cost of feedstock which resulted in that of biodiesel is a large problem to be resolved. Dark oil from industrial process can be a better alternative for biodiesel production because of its low price. In spite of this, the study of biodiesel production using the dark oil has not been reported. This study provides technical information and catalytic properties on this system. Among the several catalysts, WO 3 /ZrO 2 catalyst was the most effective catalyst in the esterification of the dark oil to fatty acid methyl esters (FAMEs). The catalytic reaction parameters were optimized that 20 wt.% WO 3 /ZrO 2 has a high FFA conversion of 96% at 150 °C, 0.4 g/ml (oil), 1:9 (oil to alcohol, molar ratio) and 2 h reaction time. The physical and chemical properties of the catalyst were characterized by XRD, Raman spectrometer, BET and NH 3 -TPD.

The current status and challenges associated with the production and utilization of cellulosic ethanol in Korea are reviewed in this paper. Cellulosic ethanol has emerged as a promising option for mitigating Korea's CO(2) emissions and enhancing its energy security. Korea's limited biomass resources is the most critical barrier to achieving its implementation targets for cellulosic ethanol. Efforts to identify new suitable biomass resources for cellulosic ethanol production are ongoing and intensive. Aquatic biomasses including macroalgae and plantation wastes collected in the Southeast Asia region have been found to have great potential as feedstocks for the production of cellulosic ethanol. R&D explorations into the development of technologies that can convert biomass materials to ethanol more efficiently also are underway. It is expected that cellulosic ethanol will be in supply from 2020 and that, by 2030, its use will have effectively reduced Korea's total gasoline consumption by 10%.

To maximize the production of biodiesel from soybean soapstock, the effects of water on the esterification of high-FFA (free fatty acid) oils were investigated. Oleic acid and high acid acid oil (HAAO) were esterified by reaction with methanol in the presence of Amberlyst-15 as a heterogeneous catalyst or sulfuric acid as a homogeneous catalyst. The yield of fatty acid methyl ester (FAME) was studied at oil to methanol molar ratios of 1:3 and 1:6 and reaction temperatures of 60 and 80 °C. The rate of esterification of oleic acid significantly decreased as the initial water content increased to 20% of the oil. The activity of Amberlyst-15 decreased more rapidly than that of sulfuric acid, due to the direct poisoning of acid sites by water. Esterification using sulfuric acid was not affected by water until there was a 5% water addition at a 1:6 molar ratio of oil to methanol. FAME content of HAAO prepared from soapstock rapidly increased for the first 30 min of esterification. Following the 30-min mark, the rate of FAME production decreased significantly due to the accumulation of water. When methanol and Amberlyst-15 were removed from the HAAO after 30 min of esterification and fresh methanol and a catalyst were added, the time required to reach 85% FAME content was reduced from 6 h to 1.8 h.

This study details the actual wearing conditions and favored design of ready-made pants for obese women, particularly for those in their twenties and thirties. The actual problems that customers feel and the preferred design of pants were suggested based on the survey and from the standpoint of consumers. The findings of the survey are as follows. The actual wearing conditions in the survey, 80.5% like wearing pants. The main reason to prefer pants is that pants are more convenient for social or professional work activities. The main reason to avoid wearing pants is that the pants (in general) do not mask an obese body shape. The most favored was Denim/Elastic and the second most favored was Cotton/Elastic from the survey. On average, elastic materials are preferred over non-elastic materials. The reason was the comfort in wearing and convenience in social or professional activities that could mask obesity. In terms of the style, the most favored was blue jeans and the second favored was semi-formal. A straight-silhouette is the most favored as it was able to help mask obesity. The most favored position for the waistline is 4 cm lower than the natural waistline. The most favored pant length is where the pants slightly touch the floor when they stand with shoes on. For obese women the pants development that covers the weak points of the body shape giving, which is comfortable and active are desired.

Dead volume classified as bed head, bed tail, and bed line affects the purity and recovery in industrial-scale simulated-moving-bed (SMB) processes. Considering the three types of dead volume, the operation strategies for four, seven, and eight zones are presented for an industrial-scale para-xylene (PX) SMB process, and their performances are compared in terms of purity, recovery, and dilution. The dead volume is treated by a Courant−Friedrichs−Lewy- (CFL-) number-insensitive method of characteristics (MOC). An extended node model integrating the dead volumes has been developed and solved by using the MOC. Seven-zone operation including line and secondary flushes enhances the purity, while keeping almost the same recovery as achieved with four-zone operation. The secondary flush introducing pure PX reduces the dilution of the extract. Eight-zone operation with both a secondary flush introducing pure PX and a tertiary flush-out shows high productivity with low dilution and over 99.7% purity.