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The Carpathian arc formed during Cretaceous to Miocene time by continental collision between Europe and smaller continental fragments following southward and westward subduction of ocean floor. During the last stages of thrusting in the outer Carpathians, a set of discrete basins formed behind the Carpathian loop. These basins are regions of middle to late Miocene extension and are connected to each other and to areas of coeval shortening in the outer Carpathian thrust belt by a system of strike-slip faults. Sinistral northeast trending and dextral northwest trending sets of conjugate shears reflect overall east-west extension of the intra-Carpathian region during middle to late Miocene time. Palinspastic reconstruction of the basins indicates about 100 (±50) km of east-west extension across the intra-Carpathian region during this time. We interpret the Vienna basin, which is superimposed partly on the flysch nappes of the outer West Carpathians, as the result of thin-skinned extensional tectonics above a shallow detachment surface within the crust. Extension of upper crustal rocks above this detachment was probably accomodated by thrusting of the outermost nappes of the West Carpathians over the European platform. Extension of basins farther inside the Carpathian loop probably involved rocks at deeper crustal levels and within the upper mantle. The most internally situated basins formed by extensional processes that involved the entire lithosphere. Thus the depth to which extension occurred seems to be closely related to the proximity of each basin to the active thrust front in the Carpathians. This can be related to the geometry of the subduction zone. Extension within the intra-Carpathian basins began first in the northernmost basins and migrated southward and eastward over a ten million year period. Palinspastic reconstruction of the basins at three different times during the Miocene (17.5, 16.5 and 13.0 Ma) predicts eastward migration of the zone of thrusting and shortening within the Carpathian mountains to accomodate the synchronous eastward and southward migration of areas of extension. From these reconstructions, about 120 (±60) km of east-west shortening is predicted across the outer East Carpathians from about 17.5 Ma until present. Both predictions are in good agreement with the timing and magnitude of shortening in the Carpathian thrust belt as determined directly from rocks within the belt. From this we infer a close relationship for middle to late Miocene extension of the intra-Carpathian region and middle to late Miocene crustal shortening within the outer Carpathians. During middle and late Miocene time, deformation of the Pannonian fragment was dominated by extensional and wrench tectonics, except along a narrow zone in the outer Carpathian thrust belt. Middle and late Miocene thrusting in the Carpathians is interpreted as the result of simple shear caused by relative motion between Europe and the overriding Pannonian fragment at the subduction boundary, rather than of regional compression of lithospheric rocks at depth. The initiation of extensional tectonics within the intra-Carpathian region in middle Miocene time can be related to reorganization of major fragment boundaries of the Eastern European Alpine system. [ABSTRACT FROM AUTHOR]

Abstract Lake-floor morphologies may be significantly different from seafloor topographies of other basins, typically observed in passive or active continental margins. The bathymetry of large paleo-lakes is often overwritten by subsequent tectonic evolution, burial beneath thick overburden and inherent compaction effects. We study the evolution of such an initial underfilled, balance fill and finally overfilled large paleo-lake basin by the interpretation of 2D and 3D seismic data set corroborated with calibrating wells in the example of the Neogene Pannonian Basin of Central Europe. Lake Pannon persisted for about 7–8 Myr and was progressively filled by clastic material sourced by the surrounding mountain chains and transported by large rivers, such as the paleo-Danube and paleo-Tisza. We combined sedimentological observations with a backstripping methodology facilitated by well lithology and porosity data to gradually remove the sediment overburden. This approach has resulted in a morphological reconstruction of the former depositional surfaces with special focus on the prograding shelf-margin slopes. Our calculations show that the water depth of the lake was more than 1000 m in the deepest sub-basins of the Great Hungarian Plain of the Pannonian Basin. The significant compaction associated with lateral variations of Neogene sediment thicknesses has created non-tectonic normal fault offsets and folds. These features have important effects on fluid migration and hydrocarbon trapping. We furthermore compare the geometries and effects of such non-tectonic features with the activity of larger offset sinistral strike-slip zones using 3D seismic attributes. Graphical abstract Image 1 Highlights • Lake Pannon shows the transition from an underfilled to an overfilled paleo-lake. • Calculations indicate the variation of water depth values from ~ 75 m up to ~ 1 km. • Sedimentary transport routes were determined by inherited and active tectonics. • Differential compaction induced folds and faults are analysed. [ABSTRACT FROM AUTHOR]

Aim: Primary forests have high conservation value but are rare in Europe due to historic land use. Yet many primary forest patches remain unmapped, and it is unclear to what extent they are effectively protected. Our aim was to (1) compile the most comprehensive European‐scale map of currently known primary forests, (2) analyse the spatial determinants characterizing their location and (3) locate areas where so far unmapped primary forests likely occur. Location: Europe. Methods: We aggregated data from a literature review, online questionnaires and 32 datasets of primary forests. We used boosted regression trees to explore which biophysical, socio‐economic and forest‐related variables explain the current distribution of primary forests. Finally, we predicted and mapped the relative likelihood of primary forest occurrence at a 1‐km resolution across Europe. Results: Data on primary forests were frequently incomplete or inconsistent among countries. Known primary forests covered 1.4 Mha in 32 countries (0.7% of Europe's forest area). Most of these forests were protected (89%), but only 46% of them strictly. Primary forests mostly occurred in mountain and boreal areas and were unevenly distributed across countries, biogeographical regions and forest types. Unmapped primary forests likely occur in the least accessible and populated areas, where forests cover a greater share of land, but wood demand historically has been low. Main conclusions: Despite their outstanding conservation value, primary forests are rare and their current distribution is the result of centuries of land use and forest management. The conservation outlook for primary forests is uncertain as many are not strictly protected and most are small and fragmented, making them prone to extinction debt and human disturbance. Predicting where unmapped primary forests likely occur could guide conservation efforts, especially in Eastern Europe where large areas of primary forest still exist but are being lost at an alarming pace. [ABSTRACT FROM AUTHOR]

Three-dimensional geophysical modelling of the early Late Miocene Pásztori volcano (ca. 11-10 Ma) and adjacent area in the Little Hungarian Plain Volcanic Field of the Danube Basin was carried out to get an insight into the most prominent intra-crustal structures here. We have used gridded gravity and magnetic data, interpreted seismic reflection sections and borehole data combined with re-evaluated geological constraints. Based on petrological analysis of core samples from available six exploration boreholes, the volcanic rocks consist of a series of alkaline trachytic and trachyandesitic volcanoclastic and effusive rocks. The measured magnetic susceptibilities of these samples are generally very low suggesting a deeper magnetic source. The age of the modelled Pásztori volcano, buried beneath a 2 km-thick Late Miocene-to-Quaternary sedimentary sequence, is 10.4 +/− 0.3 Ma belonging to the dominantly normal C5 chron. Our model includes crustal domains with different effective induced magnetizations and densities: uppermost 0.3-1.8 km thick layer of volcanoclastics underlain by a trachytic-trachyandesitic coherent and volcanoclastic rock units of a maximum 2 km thickness, with a top situated at minimal depth of 2.3 km, and a deeper magmatic pluton in a depth range of 5-15 km. The 3D model of the Danube Basin is consistent with observed high ΔZ magnetic anomalies above the volcano, while the observed Bouguer gravity anomalies correlate better with the crystalline basement depth. Our analysis contributes to deeper understanding of the crustal architecture and the evolution of the basin accompanied by alkaline intraplate volcanism. [ABSTRACT FROM AUTHOR]

Nitric oxide (NO) is one of the key elements in the complex signalling pathway leading to stomatal closure by inducing reversible protein phosphorylation and Ca2+ release from intracellular stores. As photosynthesis in guard cells also contributes to stomatal function, the aim of this study was to explore the potential role of NO as a photosynthetic regulator. This work provides the first description of the reversible inhibition of the effect of NO on guard cell photosynthetic electron transport. Pulse amplitude modulation (PAM) chlorophyll fluorescence measurements on individual stomata of peeled abaxial epidermal strips indicated that exogenously applied 450nM NO rapidly increases the relative fluorescence yield, followed by a slow and constant decline. It was found that NO instantly decreases photochemical fluorescence quenching coefficients (qP and qL), the operating quantum efficiency of photosystem II (ΦPSII), and non-photochemical quenching (NPQ) to close to zero with different kinetics. NO caused a decrease in NPQ, which is followed by a slow and continuous rise. The removal of NO from the medium surrounding the epidermal strips using a rapid liquid perfusion system showed that the effect of NO on qP and ΦPSII, and thus on the linear electron transport rate through PSII (ETR), is reversible, and the constant rise in NPQ disappears, resulting in a near steady-state value. The reversible inhibition by NO of the ETR could be restored by bicarbonate, a compound known to compete with NO for one of the two coordination sites of the non-haem iron (II) in the QAFe2+QB complex. [ABSTRACT FROM AUTHOR]

Questions: Can the climatic adaptive capacity of natural ecosystems be estimated with using landscape indicators based on vegetation or land-cover data? Can species distribution model (SDM) outputs be enhanced using such indicators? What are the data requirements and optimal parameter values of potential indicators? Location: Indicator framework: unspecified. Case study: Kiskunság, Hungary. Methods: (1) We define a general framework for handling adaptation in ecological climate change impact assessments based on IPCC definitions. (2) As a part of this general framework, we propose an indicator framework consisting of two specific indicators (landscape connectivity and landscape diversity index) to estimate adaptive capacity of ecosystems to climate change. (3) Using old-field regeneration as a proxy process, we test the proposed indicators, perform sensitivity analysis to optimize them and detect limits of their applicability. Results: Landscape metrics could provide significant information on regeneration success of old-fields. A combination of large-scale connectivity and local diversity had the highest explanatory power, with connectivity being clearly superior. The tested indicator framework can be applied on the basis of commonly available land-cover data sets. Ecological factors (like dispersal distances) are more important determinants of indicator performance than technical parameters or data resolution, provided minimum data quality is given. The dispersal distance of characteristic species of the Kiskunság forest-steppe region was ∼1 to 8 km during the last one to four decades. Conclusions: The results convincingly show that relatively simple and tractable metrics can effectively indicate the landscape-specific capacity of ecosystems to adjust to climatic changes. We argue that the use of adaptive capacity indicator frameworks consisting of simple but ecologically meaningful indicators should accompany every policy-oriented SDM study. [ABSTRACT FROM AUTHOR]

Summary: Pre-treatment with 10−4 M salicylic acid (SA) in hydroponic culture medium provided protection against salinity stress in tomato plants (Solanum lycopersicum L. cv. Rio Fuego). The effect of 10−7 or 10−4 M SA on the water status of plants was examined in relation to the biosynthesis and accumulation of abscisic acid (ABA) in order to reveal the role of SA in the subsequent response to salt stress. Both pre-treatments inhibited the K+(86Rb+) uptake of plants, reduced the K+ content of leaves, and caused a decrease in leaf water potential (ψ w). Due to the changes in the cellular water status, SA triggered the accumulation of ABA. Since the decrease in ψ w proved to be transient, the effect of SA on ABA synthesis may also develop via other mechanisms. In spite of osmotic adaptation, the application of 10−4 M, but not 10−7 M SA, led to prolonged ABA accumulation and to enhanced activity of aldehyde oxidase (AO1, EC.1.2.3.1.), an enzyme responsible for the conversion of ABA-aldehyde to ABA, both in root and leaf tissues. AO2–AO4 isoforms from the root extracts also exhibited increased activities. The fact that the activities of AO are significantly enhanced both in the leaves and roots of plants exposed to 10−4 M SA, may indicate a positive feedback regulation of ABA synthesis by ABA in this system. Moreover, during a 100mM NaCl treatment, higher levels of free putrescine or spermine were found in these leaves or roots, respectively, than in the salt-stressed controls, suggesting that polyamines may be implicated in the protection response of the cells. As a result, Na+ could be transported to the leaf mesophyll cells without known symptoms of salt toxicity. [Copyright &y& Elsevier]

The role of nitric oxide (NO) in photosynthesis is poorly understood as indicated by a number of studies in this field with often conflicting results. As various NO donors may be the primary source of discrepancies, the aim of this study was to apply a set of NO donors and its scavengers, and examine the effect of exogenous NO on photosynthetic electron transport in vivo as determined by chlorophyll fluorescence of pea (Pisum sativum) leaves. Sodium nitroprusside-induced changes were shown to be mediated partly by cyanide, and s-nitroso-N-acetylpenicillinamine provided low yield of NO. However, the effects of S-nitrosoglutathione are inferred exclusively by NO, which made it an ideal choice for this study. QA reoxidation kinetics show that NO slows down electron transfer between QA and QB and inhibits charge recombination reaction of QZ with the S2 state of the water-oxidizing complex in photosystem II. Consistent with these results, chlorophyll fluorescence induction suggests that NO also inhibits steady-state photochemical and non-photochemical quenching proceses. NO also appears to modulate reaction-center-associated nonphotochemical quenching. [ABSTRACT FROM PUBLISHER]

In this paper we present the main characteristics of a new, grid-based, landscape-ecology-oriented, satellite-image supported, field vegetation mapping method, called MÉTA (MÉTA stands for Magyarországi Élõhelyek Térképi Adatbázisa: GIS Database of the Hungarian Habitats). The goals of the MÉTA method based vegetation mapping program (MÉTA mapping) include the following: (1) to map the actual (semi-)natural vegetation of Hungary; (2) to evaluate Hungarian (semi-)natural vegetation heritage for conservation purposes; (3) to evaluate the present state of Hungarian landscapes from a vegetation point of view; (4) to collect vegetation and landscape ecological data for the prognosis of future changes of vegetation and the landscape. Spatial resolution, mapped attributes and mapping methods were developed to meet these goals. The MÉTA method uses a hexagon grid with cells of 35 hectares. In the hexagons, habitat types are listed, then the area, naturalness-based habitat quality, spatial pattern in the hexagon, effect of the neighbourhood, connectedness, and threats are recorded for each habitat type. Other attributes are recorded in the hexagons: potential natural vegetation, area occupied by invasive plant species, area of old fields, land use of grasslands, and landscape health status (naturalness and regeneration potential of the landscape in general). One hundred hexagons form a quadrat -- mainly for practical, organizational reasons, but also for collecting certain vegetation data at this spatial scale. For standardization of mapping, three different pre-printed data sheets and two different kinds of guides have been composed (Mapping Guide and Habitat Guide) and field trainings were organized. For standardization of estimation of naturalness-based habitat quality and regeneration potential field examples were prepared for each habitat type and each category of these attributes. [ABSTRACT FROM AUTHOR]

The Cumanians were originally Asian pastoral nomads who in the 13th century migrated to Hungary. We have examined mitochondrial DNA from members of the earliest Cumanian population in Hungary from two archeologically well-documented excavations and from 74 modern Hungarians from different rural locations in Hungary. Haplogroups were defined based on HVS I sequences and examinations of haplogroup-associated polymorphic sites of the protein coding region and of HVS II. To exclude contamination, some ancient DNA samples were cloned. A database was created from previously published mtDNA HVS I sequences (representing 2,615 individuals from different Asian and European populations) and 74 modern Hungarian sequences from the present study. This database was used to determine the relationships between the ancient Cumanians, modern Hungarians, and Eurasian populations and to estimate the genetic distances between these populations. We attempted to deduce the genetic trace of the migration of Cumanians. This study is the first ancient DNA characterization of an eastern pastoral nomad population that migrated into Europe. The results indicate that, while still possessing a Central Asian steppe culture, the Cumanians received a large admixture of maternal genes from more westerly populations before arriving in Hungary. A similar dilution of genetic, but not cultural, factors may have accompanied the settlement of other Asian nomads in Europe. [ABSTRACT FROM AUTHOR]

Lake Balaton (Hungary), the largest lake in Central Europe, formed by the interplay of tectonic and external forces. Its shallow water and young soft sediments together allow to carry out ultra-high resolution reflection seismic surveys to investigate shallow tectonic structures and near surface stratigraphy at depth of ca. 0–30 m. To document neotectonics of the eastern lake basin and its onshore surroundings to the East, we have integrated new ultra-high-resolution seismic-reflection data with deeper penetrating multichannel lake and land seismic profiles, regional geological, geophysical and seismicity data, and geomorphological observations. Combined use of these different data sets provided an opportunity to understand better, how these different types and scales of structural features are linked. In our study area, late middle to late Miocene formations compose a deepening than shallowing sedimentary cycle from terrestrial clastic through offshore marl and deltaic sequence filling up the basin between ca. 8.6 to 7.5 Ma. The deltaic sequence is unconformably overlain by erosional remnants of late Pleistocene fluvial deposits and a mantle of latest Pleistocene to Holocene lake mud. Post-early Miocene deformation history involved two phases; a latest middle to early late Miocene transtension and a dominantly strike-slip regime with locally transpressional or transtensional character. The latter neotectonic phase reactivated the earlier faults and resulted in the propagation of 4 major fault zones across the complete late Miocene sequence. The resulting young faults show segmented geometry, stepovers, and connecting splays. The deformation also induced the modest but penetrative folding of the highest preserved Miocene deltaic sequence. The change in deformation style could happen during the late-stage of delta formation, at ca. 8 Ma although a slightly younger timing is not excluded. Faults imaged offshore apparently do not offset the Holocene lacustrine mud by discrete fractures, but the improved distribution map of recent seismicity and morphotectonic indices along their onshore continuations suggests that several segments of the fault pattern are still active, and might be capable of generating earthquakes. Integration of these different data provided an opportunity to understand better, how these different types and scales of structural features are linked and evolved one after another. • 4 fault zones and 4 types of folds were mapped below Lake Balaton. • Post-8-Ma neotectonic faults are the reactivation of earlier Miocene structures. • The mapped fault pattern serves as an example for the complexity of segmented fault zone. • Faults below the lake continue onland form a coherent structural pattern. • Recent fault activity is suggested by the new seismicity map. [ABSTRACT FROM AUTHOR]

Background: Medical students are at risk of contracting and transmitting infectious diseases such as pertussis. Complete vaccination status is important to protect own, patient and public health. Knowing own vaccination status is elementary for following current vaccination recommendations, including boosters. We aimed to assess pertussis vaccination status and vaccination acceptance among medical students of different nationalities.Methods: A cross-sectional multicenter health survey at German and Hungarian universities enclosed international medical students in the 1st, 3rd and 5th year of study. Self-reported data from 2655 students regarding pertussis vaccination status were analyzed. Subgroup analysis enclosed data of German (n = 1217), Hungarian (n = 960) and other nationality (n = 478) students ("other").Results: More Hungarians reported basic immunization (39.0% vs 15.8% Germans vs 24.3% others, p ≤ 0.05). Booster vaccination was reported more by Germans (60.5% vs 43.6% Hungarians vs 36.0% others, p ≤ 0.05). Germans were more likely to report being unvaccinated (3.7% vs 0.9% Hungarians, p ≤ 0.05). More medical students of other nationalities were unaware of their pertussis vaccination status (37.4% vs 20.0% Germans/ 16.5% Hungarians, p ≤ 0.05). 75.2% (n = 1931) rated pertussis vaccinations as absolutely necessary (86.2% Hungarians vs 69.8% Germans/ 66.1% others, p ≤ 0.05).Conclusions: Positive attitudes towards vaccinations were reported but a large group reported insufficient vaccination status and being not aware of their status, especially among international students. Hungarians possibly have a better vaccination status than reported, based on mandatory vaccinations in childhood. The low awareness of vaccination status has implications for future booster vaccinations. All students should be informed about current recommendations and receive vaccination offers in frames of low-threshold medical services. [ABSTRACT FROM AUTHOR]

Background: Tobacco use is the leading preventable cause of death worldwide. Besides cigarette smoking, waterpipe and e-cigarettes are gaining popularity among young adults. Medical students' smoking behavior is of particular interest because of their impending role in health promotion as future physicians. Aim of our study is to examine the prevalence and predictors of cigarette, waterpipe and e-cigarette use and the association of tobacco use with self-reported health status in an international sample of medical students.Methods: In a multicenter cross-sectional study data on different aspects of health behavior were collected from medical students of 65 nationalities using a self-administered questionnaire in Germany (Dresden, Munich) and Hungary (Budapest, Pécs). The survey was conducted among 1st, 3rd and 5th year students. To explore associations between smoking behavior and socio-cultural factors Pearson's chi2-tests and multivariate binary logistic regression analyses were performed.Results: The largest subpopulations were formed by German (n = 1289), Hungarian (n = 1055) and Norwegian (n = 147) students. Mean age was 22.5 ± 3.3 years. Females represented 61.6% of the sample. In the whole sample prevalence of cigarette smoking was 18.0% (95% CI 16.6-19.4%), prevalence of waterpipe use was 4.8% (95% CI 4.0-5.7%), that of e-cigarette 0.9% (95% CI 0.5-1.2%). More males (22.0%) than females (15.5%) reported cigarette smoking. The lowest prevalence of cigarette smoking was found among Norwegian students (6.2%). Cigarette smokers were older, waterpipe users were younger than non-users. E-cigarette use was not associated with age of the students. Religious involvement was protective only against cigarette smoking. Financial situation showed no association with any kind of tobacco consumption. Cigarette smokers and e-cigarette users were less likely to report very good or excellent health status.Conclusions: Cigarette smoking is still the most popular way of consuming tobacco, although alternative tobacco use is also prevalent among medical students. To further health consciousness, medical schools should pay more attention to students' health behavior, especially their smoking habits. Tobacco prevention and cessation programs for medical students should consider not only the health risks of cigarette smoking but the need to discourage other forms of tobacco use, such as waterpipe. [ABSTRACT FROM AUTHOR]

The existing plant trait databases' applicability is limited for studies dealing with the flora and vegetation of the eastern and central part of Europe and for large-scale comparisons across regions, mostly because their geographical data coverage is limited and they incorporate records from several different sources, often from regions with markedly different climatic conditions. These problems motivated the compilation of a regional dataset for the flora of the Pannonian region (Eastern Central Europe). PADAPT, the Pannonian Dataset of Plant Traits relies on regional data sources and collates data on 54 traits and attributes of the plant species of the Pannonian region. The current version covers approximately 90% of the species of the region and consists of 126,337 records on 2745 taxa. By including species of the eastern part of Europe not covered by other databases, PADAPT can facilitate studying the flora and vegetation of the eastern part of the continent. Although data coverage is far from complete, PADAPT meets the longstanding need for a regional database of the Pannonian flora., (© 2023. Springer Nature Limited.)

Deeper understanding on natural forest dynamics requires long-term data series from forests that have not been affected by human interventions, which are often scarce especially in the Pannonian Bioregion. Unmanaged, but regularly inventoried forest reserves provide an opportunity to fill this gap. The dataset provides repeated inventory data for 233 permanent plots situated in the core areas of six forest reserves selected from primary forests (Kékes), long abandoned forests (Kecskés-galya, Szalafő, Várhegy) and abandoned ones (Hidegvíz-völgy, Nagy Istrázsa-hegy). The sampled old stands represent the four most widespread hilly forest types in Hungary: Carpathian submountainous beech forest; sessile oak-hornbeam forest; Turkey oak and sessile oak forest; downy oak forest. In each plot, stand level attributes included main mensuration variables (canopy closure, stand height, tree density, basal area, living and dead volume, lying deadwood and admixture of the main tree species). Tree level attributes (diameter at breast height, height measured and estimated, crown position in the canopy, health status, tree history of all trees or shrubs having diameter larger or equal to 5 cm) were also measured in two inventories (after 6-16 years) for a total of 6,986 individual trees sampled in all plots. Fagus sylvatica L., Quercus petraea agg., Q. cerris L., Q. pubescens Willd., Carpinus betulus L., Acer campestre L. and Cornus mas L. were the most abundant. The individual tree history classification refers to regeneration ingrowth, growing phase, mortality, decaying phase and disappearance events, that can be used for calculation of various stand dynamics attributes. The dataset offers valuable opportunities for quantifying changes in stand structures and tree population dynamic attributes after the abandonment of management. Inventory data can be integrated with environmental and climatic information to understand the drivers of forest stand dynamics under a changing climate., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Author(s).)

The etiological factors of squamous cell carcinomas of the head and neck have been well known for a long time. It is also well known that the incidence of oral cancer diagnosed in younger patients is on the rise. Due to the young age of these patients, the increase in the number of these cases and the fact that many of them neither smoke nor drink alcohol it has been suggested that other factors might be at play in the carcinogenesis of oral cancer. Thus, along the classic etiological factors of smoking and alcohol abuse certain molecular marker anomalies and the human papilloma virus (HPV) have emerged as potential factors. The aim of the present study is to verify the potential prognostic factors and to map the differences in biomarker expression between the young and the old patient groups. In the present study the immunohistochemical profile of samples obtained from oral squamous cell carcinomas was studied and compared with various clinico-pathological parameters. In 88 samples the expressions of p16, p53, Ki67, EGFR were studied with a tissue microarray technique under standard reaction conditions as well as the detection and typing of HPV infection with the Full Spectrum HPV DNA method. The biomarker expression profile of young patients with oral squamous cell carcinoma was compared to that of older patients (above 50). A significant difference was found between the immunohistochemical profile of the young and old patient groups in p16, Ki67 expression. The overall survival and progression free survival were influenced by p16 expression in young age., Competing Interests: MB was employed by Synlab Genoid Molecular Diagnostic Laboratory. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Csurgay, Zalatnai, Benczik, Csomó, Horváth, Lőrincz, Komlós and Németh.)

Background: The aetiology of oral cancer is multifactorial, as various risk factors (genetics, socioeconomic and lifestyle factors) contribute to its development. Data in the literature suggest that people with periodontal disease have an increased risk of developing oral cancer, and the severity of periodontitis correlates with the appearance of oral squamous cell carcinoma. The aim of this study was to revise the non-genetic risk factors that may influence the development of OC, while focusing on the dental and periodontal status and OH., Methods: Two hundred patients (hundred diagnosed with oral cancer and hundred without oral cancer) were enrolled in our case-control study, to evaluate the association between oral cancer and the presence and severity of periodontitis, while examining several risk factors that might be responsible for oral cancer formation. A questionnaire customised for oral cancer patients was used to obtain the socioeconomic and lifestyle risk factors that may influence the development of oral squamous cell carcinoma. The dental and periodontal status along with the level of oral hygiene was recorded quantitatively. The chi-square and Mann-Whitney tests and logistic regression were used for the statistical analysis., Results: By considering both the case and the control groups, a significant correlation was found between the incidence of oral cancer and some socioeconomic factors and lifestyle habits, such as the sex, age, education and alcohol consumption of an individual. The mean value of the Silness-Löe plaque index was significantly higher in the case population. The number of completely edentulous patients was higher among the oral cancer population. The incidence of oral cancer was 57.1% in patients with periodontal disease. In comparison, the incidence of oral squamous cell carcinoma was only 28.6% among the patients without periodontitis. Most of the oral cancer patients (72.1%) had stage 4 periodontitis. On the other hand, the vast majority of the control group (51.6%) had stage 2 periodontitis., Conclusion: Periodontitis can be an individual risk factor for oral cancer development. Periodontally compromised individuals should be strictly monitored, especially those with severe periodontitis and coexisting lifestyle risk factors. Maintaining their periodontal health in at-risk patients can minimize cancer risks., (© 2021. The Author(s).)

Objective: Delayed-onset infection is defined as infectious swelling and trismus accompanied by pain or the presence of suppuration starting approximately 30 days after surgery. This study aimed to describe the occurrence and potential predisposing factors of delayed-onset infection., Study Design: A retrospective case-control study of 223 lower third molar surgeries was performed. Participants were selected from among 1102 outpatients who underwent surgery between January 2013 and June 2018 at Semmelweis University. The inclusion criterion for the case group was inflammation of the operated area after suture removal. Patients in the control group were healthy nonsmokers <26 years old who healed without complication. Statistical analysis was performed using the Shapiro-Wilk test, the Mann-Whitney U test, and Fisher's exact test., Results: Complications occurred only in patients <26 years old approximately 29.5 days after surgery. A significantly higher risk was observed for younger age, total soft tissue coverage, deeper impaction, lower Nolla stage (P < .001), mesioangular direction (P = .002), and full bone coverage (P < .05). Distal space was inversely correlated with complications (P < .001)., Conclusions: Lower Nolla stage, total soft tissue coverage, lack of distal space, deeper impaction, or mesioangular tilt may promote delayed-onset infection. Follow-up of at-risk patients and the maintenance of oral hygiene are recommended., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Discrimination and species identification of meat has always been of paramount importance in the European meat market. This is often achieved using different conventional analytical methods but advanced sensor-based methods, such as the electronic tongue (e-tongue), are also gaining attention for rapid and reliable analysis. The aim of this study was to discriminate Angus, domestic buffalo, Hungarian Grey, Hungarian Spotted cattle, and Holstein beef meat samples from the chuck steak part of the animals, which mostly contained longissimus dorsi muscles, using e-tongue as a correlative technique with conventional methods for analysis of pH, color, texture, water activity, water-holding capacity, cooking yield, water binding activity, and descriptive sensory analysis. Analysis of variance (ANOVA) was used to determine significant differences between the measured quality traits of the five-meat species after analysis with conventional analytical methods. E-tongue data were visualized with principal component analysis (PCA) before classifying the five-meat species with linear discriminant analysis (LDA). Significant differences were observed among some of the investigated quality parameter. In most cases, Hungarian Grey was most different from the other species. Using e-tongue, separation patterns could be observed in the PCA that were confirmed with 100% recognition and 97.5% prediction of all the different meat species in LDA.

Primary forests, defined here as forests where the signs of human impacts, if any, are strongly blurred due to decades without forest management, are scarce in Europe and continue to disappear. Despite these losses, we know little about where these forests occur. Here, we present a comprehensive geodatabase and map of Europe's known primary forests. Our geodatabase harmonizes 48 different, mostly field-based datasets of primary forests, and contains 18,411 individual patches (41.1 Mha) spread across 33 countries. When available, we provide information on each patch (name, location, naturalness, extent and dominant tree species) and the surrounding landscape (biogeographical regions, protection status, potential natural vegetation, current forest extent). Using Landsat satellite-image time series (1985-2018) we checked each patch for possible disturbance events since primary forests were identified, resulting in 94% of patches free of significant disturbances in the last 30 years. Although knowledge gaps remain, ours is the most comprehensive dataset on primary forests in Europe, and will be useful for ecological studies, and conservation planning to safeguard these unique forests., (© 2021. The Author(s).)

Background: Various surface treatment options have been adopted with the aim to improve osseointegration, reducing the overall treatment time. Implant stability of early loaded implants with different modified surfaces was compared in the present study., Methods: Patients were selected from the Department of Oro-Maxillofacial Surgery and Stomatology at Semmelweis University. Patients randomly received SA (alumina sandblasted and acid-etched), NH (bioabsorbable apatite nanocoating) or SLA (large-grit sandblasted and acid-etched) surface implants. Outcome measures were: implant success, implant stability, and periodontal parameters. The implant stability was measured at the time of implant placement (primary stability) and six weeks after (prothesis delivery, secondary stability). Osstell and Periotest were applied to take all the measurements. The primary and secondary stability were compared in the three study groups Finally the periimplant probing depth appearing after three months of loading was checked on 6 points around to the implant-supported prostheses. Shapiro-Wilk and Mann-Whitney tests were used for the comparison between the study groups., Results: A total of 75 implants with different length and diameter were inserted into various positions. One implant failed spontaneously at the fourth week after implant placement. The survival rate was 98,7%. Comparing the primary and secondary stability values, the data were significantly improved in every groups. The difference was the highest in the NH group, however, this difference was not significant compared to the two other groups. Good periodontal parameters were experienced in all the tested implants, independently by the groups., Conclusions: With the limitation of the present study, all the implants showed improved stability six weeks after implant placement. A trend of higher result was found for the NH group. Further studies with longer follow-up are needed to confirm this preliminary results., Trial Registration: Current Controlled Trials ISRCTN13181677; the date of registration: 04/03/2021. Retrospectively registered.

Introduction and Aim: Delayed-onset infection was defined as an infectious swelling and trismus accompained by pain or the presence of suppuration starting after the discharge of the patient, generally a week after surgery. Our aim was to describe incidence and possible risk factors of delayed-onset infection after lower wisdoom tooth removal., Material and Methods: Retrospective study of 851 lower third molar surgeries, performed between January 2013 and August 2017 at Semmelweis University Faculty of Dentistry, Department of Oro-Maxillofacial Surgery and Stomatology, has been done. The teeth were removed by 2 surgeons. Under age of 26, a case-control study comprising a total of 150 lower third molar surgeries were done to indentify possible risk factors., Results: 10 delayed-onset wound infections were recorded after suture removal (1.17% of the patients). The mean time elapsing from surgery to delayed-onset infection was 30 days. Lower third molars with total soft tissue coverage, a bigger amount of bone coverage, a lack of distal space (Pell-Gregory class III), deeper impaction (Pell-Gregory classes B, C) or mesioangular tilt are associated with the development of this complication (p<0.05). Female sex and young age seem to be risk factors, too., Conclusion: Patients with risk factors should be warned that infection may still occur several weeks after surgery. The clinicians should observe these patients longer and emphasize keeping their wound clean for a prolonged period. Orv Hetil. 2018; 159(31): 1278-1283.

Ancient DNA studies have established that Neolithic European populations were descended from Anatolian migrants who received a limited amount of admixture from resident hunter-gatherers. Many open questions remain, however, about the spatial and temporal dynamics of population interactions and admixture during the Neolithic period. Here we investigate the population dynamics of Neolithization across Europe using a high-resolution genome-wide ancient DNA dataset with a total of 180 samples, of which 130 are newly reported here, from the Neolithic and Chalcolithic periods of Hungary (6000-2900 bc, n = 100), Germany (5500-3000 bc, n = 42) and Spain (5500-2200 bc, n = 38). We find that genetic diversity was shaped predominantly by local processes, with varied sources and proportions of hunter-gatherer ancestry among the three regions and through time. Admixture between groups with different ancestry profiles was pervasive and resulted in observable population transformation across almost all cultural transitions. Our results shed new light on the ways in which gene flow reshaped European populations throughout the Neolithic period and demonstrate the potential of time-series-based sampling and modelling approaches to elucidate multiple dimensions of historical population interactions.

Introduction and Aim: The study compares the antibiotic prophylaxis combined with postoperative antibiotic therapy to preoperative chlorhexidine rinse combined with postoperative antibiotic therapy in preventing complications after surgical removal of a mandibular third molar., Method: 71 healthy patients in four groups were enrolled in the study: I. prophylactic dose of 2000 mg of amoxicillin clavulanate, continued with amoxicillin clavulanate postoperatively; II. prophylactic dose of 600 mg of clindamycin, continued with clindamycin postoperatively; III. prophylactic chlorhexidin rinsing, continued randomized amoxicillin clavulanate or clindamycin postoperatively; IV. control, with clindamycin postoperatively., Results: The pain was smaller in the prophylaxis groups. Alveolitis occurred only in the control group: 2 patients. Wound opening occurred in 22,2 % in group IV., 14,2 % in group II, 10 % in group I., 5 % in group III., Conclusion: We consider completing the indicated postoperative antibiotic prescription with antibiotic or antiseptic prophylaxis. Chlorhexidin prophylaxis could have the same positive effect. Orv. Hetil., 2017, 158(1), 13-19.

Introduction: The health status of doctors, nurses and any other professionals working in the healthcare sector influences the quality of their work. Therefore, health promotion of healthcare workers is not only an occupational health program but it can be considered as an action towards improving the quality of care., Aim: The current study was designed to assess the efficacy of a health promotion intervention among healthcare workers after health status assessment., Method: Before and after the intervention a self-assessment questionnaire-based health survey and physical status examination were performed. Members of the intervention group were offered to use fitness facilities, professional psychological help, dietary counselling, and dental consultation., Results: The intervention program lasted for 12 months with the participation of 79 health care professionals (based on their decision being in the intervention or in the control group). Significant decrease was found in the abdominal perimeter in participants of the intervention group and they ran a longer distance on the 12 minute-long-run test at the end of the study., Conclusions: The positive effect of regular physical exercise as part of active lifestyle has been demonstrated not only in the increase of physical capacity, but in the change of body shape, as well. Orv. Hetil., 2016, 157(39), 1563-1570.

Introduction: The changing of the family medicine can be observed in the New Millennium. Migration, the aging of the healers and informal payment are crucial to the human resource crisis of the health sector., Aim: The aim of this study was to investigate the family physicians' and residents' opinions about the vocation and informal payment., Method: Exploratory, quantitative study was carried out among family physicians (n = 363) and family physician residents (n = 180). The central questions of the study were the vocation, the income and the informal payment., Results: The most decisive factors of the carrier choice were altruism, service and responsibility. Residents were significantly rejective (19.7% vs. 38.3%, p<0.001) about informal payment. They would accept smaller amounts of informal payment (14.3% vs. 8.9%, p<0.034), and would spend it on praxis development (1.4% vs.9.4% p<0.023)., Conclusions: The attitudes of family physicians and residents are the same in case of the vocation, but on the issue of informal payment, the two generations have different opinions. Orv. Hetil., 2016, 157(36), 1438-1444.

The present study summarizes the most important events of life and professional career of Daniel Józsa born 220 years ago. Józsa, the Medical Chief of former Crasna county was born in Uzon of Székely region on 1, October, 1795. From the age of 10 he studied at the Bethlen College in Nagyenyed. In 1822 he attended the University of Medical Sciences in Vienna, where he got his doctor's degree in 1828. He wrote his dissertation on the diseases of the female breast. In the same year he settled with his first wife in Szilágysomlyó, Crasna county, where he was appointed Medical Chief of the county by the Transylvanian Governor. He gained great medical reputation being a lively, correct and energetic man with rational medical principles ahead of his time. He took part and held a presentation at the general meeting of the Hungarian physicians and nature researchers in Kolozsvár in 1844. As a result, he was elected associate member by both the Royal Hungarian Natural Science Society and the Royal Medical Association of Budapest. While he successfully fought against the spread of cholera during the epidemics of 1831, 1836 and 1848-49, he regularly took part in the public life of the county. From his first wife he had 6 children, from his second wife he had one child. He died in the village Bályok in Bihar county on 10, April, 1849. His tomb has been saved for future generations by the generosity of the local Reformed Church and of some doctors from Bályok.

Structural analysis of K+ channel pores suggests that the selectivity filter of the pore is an inherent sensor for extracellular K+ (Ko+); channels seem to be inactivated at low Ko+ because of a destabilization of the conducting state and a collapse of the pore. In the present study, the effect of depleting Ko+ on the activity of a plant K+ channel, KAT1, from Arabidopsis thaliana was investigated. This channel is thought to be insensitive to Ko+. The channel was therefore expressed in mammalian HEK293 cells and measured with patch clamp technology in the whole cell configuration. The effect of Ko+ depletion on channel activity was monitored from the tail currents before, during, and after washing Ko+ from the medium. The data show that a depletion of Ko+ results in a decrease in channel conductance, irrespective of whether K+ is simply removed or replaced by either Na+ or Li+. Quantitative analysis suggests that the channel has two binding sites for K+ with the dissociation constant in the order of 20 microM. This high sensitivity of the channel to Ko+ could serve as a safety mechanism, which inactivates the channel at low Ko+ and, in this way, prevents leakage of K+ from the cells via this type of channel.