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As a typical glycolytic inhibitor, 3-bromopyruvate (3-BrPA) has been extensively studied in cancer therapy in recent decades. However, few studies focused on 3-BrPA in regulating the growth and development of insects, and the relationship and regulatory mechanism between glycolysis and chitin biosynthesis remain largely unknown. The Hyphantria cunea, named fall webworm, is a notorious defoliator, which caused a huge economic loss to agriculture and forestry. Here, we investigated the effects of 3-BrPA on the growth and development, glycolysis, carbohydrate homeostasis, as well as chitin synthesis in H. cunea larvae. To elucidate the action mechanism of 3-BrPA on H. cunea will provide a new insight for the control of this pest. The results showed that 3-BrPA dramatically restrained the growth and development of H. cunea larvae and resulted in larval lethality. Meanwhile, we confirmed that 3-BrPA caused a significant decrease in carbohydrate, adenosine triphosphate (ATP), pyruvic acid (PA), and triglyceride (TG) levels by inhibiting glycolysis in H. cunea larvae. Further studies indicated that 3-BrPA significantly affected the activities of hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), glucose 6-phosphate dehydrogenase (G6PDH) and trehalase, as well as expressions of the genes related to glycolysis, resulting in carbohydrate homeostasis disorder. Moreover, it was found that 3-BrPA enhanced 20-hydroxyecdysone (20E) signaling by upregulating HcCYP306A1 and HcCYP314A1, two critical genes in 20E synthesis pathway, and accelerated chitin synthesis by upregulating transcriptional levels of genes in the chitin synthesis pathway in H. cunea larvae. Taken together, our findings provide a novel insight into the mechanism of glycolytic inhibitor in regulating the growth and development of insects, and lay a foundation for the potential application of glycolytic inhibitors in pest control as well.

B10 cells are regulatory B cells capable of producing IL-10 for maintaining immune homeostasis. Dysregulation of B10 cells occurs in autoimmune and inflammatory diseases. Modulation or adoptive transfer of B10 cells is a promising therapeutic strategy. The short-chain fatty acids (SCFAs), the metabolites of microbiota, play a critical role in maintaining immune homeostasis and are the potential drugs for the modulation of B10 cells. It is not clear whether and how SCFAs upregulate the frequency of B10 cells. Here, we found that SCFAs could promote murine and human B10 cell generation in vitro. Upregulation of B10 cells by butyrate or pentanoate was also observed in either healthy mice, mice with dextran sodium sulfate (DSS)-induced colitis, or mice with collagen-induced arthritis. Moreover, SCFA treatment could ameliorate clinical scores of colitis and arthritis. Adoptive transfer of B cells pretreated with butyrate showed more alleviation of DSS-induced colitis than those without butyrate. A further study demonstrates that SCFAs upregulate B10 cells in a manner dependent on their histone deacetylase (HDAC) inhibitory activity and independent of the G-protein-coupled receptor pathway. Transcriptomic analysis indicated that the MAPK signaling pathway was enriched in B10 cells treated with butyrate. A study with inhibitors of ERK, JNK, and p38 MAPK demonstrated that activating p38 MAPK by butyrate is critical for the upregulation of B10 cells. Moreover, HDAC inhibitor has similar effects on B10 cells. Our study sheds light on the mechanism underlying B10 cell differentiation and function and provides a potential therapeutic strategy with SCFAs and HDAC inhibitors for inflammation and autoimmune diseases.

Gold complexes have been recognized as potential anticancer agents against various kinds of diseases due to their inherent suppressions of antioxidant thioredoxin reductase (TrxR) activity. Herein, a powerful aggregation-induced emission luminogen (AIEgen), TBP-Au, was designed and synthesized by integrating an anticancer Au(I) moiety with an AIE-active photosensitizer (TBP), in which both the production and consumption routes of reactive oxygen species (ROS) were elaborately considered simultaneously to boost the anticancer efficacy. It has been demonstrated that TBP-Au could realize superior two-photon fluorescence imaging in tumor tissues with high resolution and deep penetration as well as long-term imaging in live animals due to its AIE property. In addition, the introduction of a special Au(I) moiety could tune the organelle specificity and efficiently facilitate the ROS-determined photodynamic therapy (PDT). More impressively, TBP-Au could efficiently eliminate cancer cells under light irradiation through the preconceived synergetic approaches from the PDT and the effective suppression of TrxR, demonstrating that TBP-Au holds great potential for precise cancer theranostics.

Periodontitis is a common chronic inflammatory disease that can result in tooth loss and poses a risk to systemic health. Lymphocytes play important roles in periodontitis through multiple mechanisms. Regulatory lymphocytes including regulatory B cells (Bregs) and T cells (Tregs) are the main immunosuppressive cells that maintain immune homeostasis, and are critical to our understanding of the pathogenesis of periodontitis and the development of effective treatments. In this review, we discuss the phenotypes, roles, and modulating strategies of regulatory lymphocytes including Bregs and Tregs in periodontitis and frequently cooccurring inflammatory diseases such as rheumatoid arthritis, Alzheimer disease, diabetes mellitus, and stroke. The current evidence suggests that restoring immune balance through therapeutic targeting of regulatory lymphocytes is a promising strategy for the treatment of periodontitis and other systemic inflammatory diseases.

Periodontitis induced by bacteria especially Porphyromonas gingivalis (P. gingivalis) is the most prevalent microbial disease worldwide and is a significant risk factor for systemic diseases such as rheumatoid arthritis (RA). RA and periodontitis share similar clinical and pathologic features. Moreover, the prevalence of RA is much higher in patients with periodontitis than in those without periodontitis. To explore the immunologic mechanism of periodontitis involved in RA, we established a mouse model of periodontitis and then induced RA. According to the results of paw thickness, arthritis clinical score, arthritis incidence, microscopic lesion using H&E staining, and micro-CT analysis, periodontitis induced by P. gingivalis promoted the occurrence and development of collagen-induced arthritis (CIA) in mice. Furthermore, periodontitis enhanced the frequency of CD19+ B cells, Th17, Treg, gMDSCs, and mMDSCs, whereas down-regulated IL-10 producing regulatory B cells (B10) in CIA mice preinduced for periodontitis with P. gingivalis. In vitro stimulation with splenic cells revealed that P. gingivalis directly enhanced differentiation of Th17, Treg, and mMDSCs but inhibited the process of B cell differentiation into B10 cells. Considering that adoptive transfer of B10 cells prevent RA development, our study, although preliminary, suggests that down-regulation of B10 cells may be the key mechanism that periodontitis promotes RA as the other main immune suppressive cells such as Treg and MDSCs are up-regulated other than down-regulated in group of P. gingivalis plus CIA.

The development of dark pro‐aggregation‐induced‐emission luminogens (pro‐AIEgens) based on the solid‐state intramolecular motion–caused quenching effect has been rarely reported in biochemical analysis. Herein, an azo‐conjugated pro‐AIEgen derived from tetraphenylethene is constructed. Due to the active molecular motion of the azo bond, it retains a nonemissive property in both solution and solid forms. The obtained pro‐AIEgen can be internalized by living cells and undergo reductase‐modulated derotation under hypoxia to emit light. It shows increasing fluorescence when the intracellular oxygen concentration becomes lower, making it a potential probe to sense the hypoxic environment. The present probe can also visualize the hypoxic environment created by photodynamic therapy treatment.

The study on the fast-growing traits of trees, mainly valued by tree height (TH) and diameter at breast height (DBH), is of great significance to promote the development of the forest industry. Quantitative trait locus (QTL) mapping based on high-density genetic maps is an efficient approach to identify genetic regions for fast-growing traits. In our study, a high-density genetic map for the F1 population was constructed. The genetic map had a total size of 5,484.07 centimorgan (cM), containing 5,956 single nucleotide polymorphisms (SNPs) based on Specific Length Amplified Fragment sequencing. Six fast-growing related stable QTL were identified on six chromosomes, and five stable QTL were identified by a principal component analysis (PCA). By combining the RNA-seq analysis for the two parents and two progenies with the qRT-PCR analysis, four candidate genes, annotated as DnaJ, 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), Caffeic acid 3-O-methyltransferase 1 (COMT1), and Dirigent protein 6 (DIR6), that may regulate height growth were identified. Several lignin biosynthesis-related genes that may take part in height growth were detected. In addition, 21 hotspots in this population were found. The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of TH and DBH.

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from freshwater of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30 ℃ in medium containing 0-0.75%(w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C16:1ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main ubiquinone was Q-10. The G + C content of genomic DNA was 69.8 mol%. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to M. pamukkalensis JCM 30229T with 95.9% sequences similarity. Based on taxonomic results in this study, we proposed that strain L22T was placed into a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (= KCTC 72858T = MCCC 1H00432T).

Intraarticular injection of hyaluronic acid (HA) for viscosupplementation is a nonsurgical therapy for osteoarthritis (OA). However, HA fails to lubricate under a significant load and tends to be depolymerized by the overproduction of reactive oxygen species (ROS) in inflammation. Here, we for the first time reported that fullerene-like MoS

The Chinese CubeSat Mission, Gamma Ray Integrated Detectors (GRID), recently detected its first gamma-ray burst, GRB 210121A, which was jointly observed by the Gravitational wave high-energy Electromagnetic Counterpart All-sky Monitor (GECAM). This burst is confirmed by several other missions, including \fermi and \textit{Insight}-HXMT. We combined multi-mission observational data and performed a comprehensive analysis of the burst's temporal and spectral properties. Our results show that the burst is relatively special in its high peak energy, thermal-like low energy indices, and large fluence. By putting it to the $E_{\rm p}$-$E_{\rm\gamma, iso}$ relation diagram with assumed distance, we found this burst can be constrained at the redshift range of [0.3,3.0]. The thermal spectral component is also confirmed by the direct fit of the physical models to the observed spectra. Interestingly, the physical photosphere model also constrained a redshift of $z\sim$ 0.3 for this burst, which help us to identify a host galaxy candidate at such a distance within the location error box. Assuming the host galaxy is real, we found the burst can be best explained by the photosphere emission of a typical fireball with an initial radius of $r_0\sim$ 3.2 $\times 10^7$ cm., Comment: ApJ accpeted; 12 pages, 11 figures, 3 tables

In order to achieve accurate gesture control, a gesture detection module is designed based on the infrared obstacle avoidance module array, and the robot gesture trajectory control is studied. AT89S52 single chip microcomputer is used as the upper computer to complete the collection and recognition of gesture motion through gesture detection module, which sends the processed moving target vector of the robot to the lower computer MCU STC89C52 through HC-12 wireless module, and then the operation of the mobile robot is controlled by L298N. The experimental results show that the system has high gesture recognition rate, and the mobile robot can run stably under the given trajectory of gesture, which verifies the practicability of the gesture detection module design and the effectiveness of the control system.

We performed a systematic search for X-ray bursts of the SGR J1935+2154 using the Fermi Gamma-ray Burst Monitor continuous data dated from Jan 2013 to October 2021. Eight bursting phases, which consist of a total of 353 individual bursts, are identified. We further analyze the periodic properties of our sample using the Lomb-Scargle periodogram. The result suggests that those bursts exhibit a period of ~ 238 days with a ~ 63.2% duty cycle. Based on our analysis, we further predict two upcoming active windows of the X-ray bursts. Since July 2021, the beginning date of our first prediction has been confirmed by the ongoing X-ray activities of the SGR J1935+2154., Comment: ApJL published; 16 pages, 11 figures, 3 tables

Gamma-ray bursts have been phenomenologically classified into long and short populations based on whether the observed duration is longer or shorter than two seconds. Multi-wavelength and multi-messenger observations in recent years have revealed that in general long GRBs originate from massive star core collapse events, whereas short GRBs originate from binary neutron star mergers. It has been known that the duration criterion is sometimes unreliable, and multi-wavelength criteria are needed to identify the physical origin of a particular GRB. Some apparently long GRBs have been suggested to have a neutron star merger origin, whereas some apparently short GRBs have been attributed to genuinely long GRBs whose short, bright emission is above the detector's sensitivity threshold. Still, there has been no known case that a GRB is genuinely short but originates from death of a massive star. Here we report the comprehensive analysis of the multi-wavelength data of a bright short GRB 200826A. This burst has a sharp 1-second spike, which is not part of an underlying long-duration event. Its other observational properties are, however, inconsistent with those of other short GRBs believed to originate from binary neutron star mergers. Rather, these properties resemble those of long GRBs. This burst presents a challenge to the traditional GRB classification scheme and reveals a class of core-collapse-origin GRBs with genuinly short durations.

The development of dark pro-AIEgens based on solid-state intramolecular motion-caused quenching effect has been rarely reported in biochemical analysis. Herein, an azo-conjugated pro-AIEgen derived from tetraphenylethene is constructed. Owing to the active photoisomerization of the azo bond, it retains a non-emissive property in both solution and solid forms. The obtained pro-AIEgen can be internalized by living cells and undergo reductase-modulated derotation under hypoxia to emit the light. It shows increasing fluorescence when intracellular oxygen concentration becomes lower, making it a potential probe to sense the hypoxic environment. The present probe can also visualize the hypoxic environment created by photodynamic therapy treatment.

High‐throughput gene sequencing has identified various genetic variants as the culprits for some common hereditary cancers. However, the heritability of a substantial proportion of cancers remains unexplained, which may result from rare deleterious mutations hidden in a myriad of nonsense genetic variations. This poses a great challenge to the understanding of the pathology and thus the rational design of effective treatments for affected patients. Here, whole genome sequencing is employed in a representative case in which one monozygotic twin is discordant for lung inflammatory myofibroblastoma to disclose rare tumor‐related mutations. A missense single nucleotide variation rs61955126 T>C in the lysine methyltransferase SETD8 (accession: NM_020382, SETD8C302R) is exposed. It is shown that SETD8 is vital for genomic integrity by promoting faithful DNA replication, and its C302R mutation downregulates the p53/p21 pathway. Importantly, the SETD8C302R mutation significantly increases the sensitivity of cancer cells to WEE1 inhibition. Given that WEE1 inhibitors have shown great promise for clinical approval, these results impart a potential therapeutic approach using WEE1 inhibitor for cancer patients carrying the same mutation, and indicate that genome sequencing and genetic functional studies can be integrated into individualized therapies., Whole genome sequencing on a pair of identical twins discordant for inflammatory myofibroblastoma and following functional studies reveal a missense single nucleotide variation (rs61955126 T>C) in the lysine methyltransferase SETD8 (accession: NM_020382, SETD8C302R). SETD8 promotes faithful DNA replication and genomic integrity, whereas SETD8C302R mutation downregulates the p53/p21 pathway and increases sensitivity of cancer cells to WEE1 inhibition.

Accompanied by an X-ray burst, the fast radio burst (FRB) FRB 200428 was recently confirmed as originating from the Galactic magnetar soft gamma repeater (SGR) SGR J1935+2154. Just before and after FRB 200428 was detected, the Five-hundred-meter Aperture Spherical radio Telescope (FAST) had been monitoring SGR J1935+2154 for eight hours. From UTC 2020 April 27 23:55:00 to 2020 April 28 00:50:37, FAST detected no pulsed radio emission from SGR J1935+2154, while Fermi/Gamma-ray Burst Monitor registered 34 bursts in the X/soft $\gamma$-ray band, forming a unique sample of X-ray bursts in the absence of FRBs. After a comprehensive analysis on light curves, time-integrated, and time-resolved spectral properties of these FRB-absent X-ray bursts, we compare this sample with the FRB-associated X-ray burst detected by Insight-HXMT, INTEGRAL, and Konus-Wind. The FRB-associated burst distinguishes itself from other X-ray bursts by its nonthermal spectrum and a higher spectral peak energy, but otherwise is not atypical. We also compare the cumulative energy distribution of our X-ray burst sample with that of first repeating FRB source, FRB 121102, with the calibration of FRB 200428-X-ray burst association. We find a similarity between the two, offering indirect support of the magnetar origin of cosmological FRBs. The event rate density of magnetar bursts is about $\sim 150$ times higher than the FRB event rate density at the energy of FRB 200428. This again suggests that, if all FRBs originate from magnetars, only a small fraction of X-ray bursts are associated with FRBs., Comment: 18 pages, 3 tables, 8 figures. Published in the Astrophysical Journal Letter on January 12, 2021

Background: To date, the medical resources and the important clinical risk factors of non-severe patients with the 2019 coronavirus disease (COVID-19) have not been investigated.Methods: we retrospectively analyszed 104 patients with COVID-19 admitted to the isolation ward in Hubei, China, from February to March 2020. The general information, clinical symptoms, blood test and CT imaging characteristics were analyzed. Results: The median length of stay (LOS) is 14 days, and the cost of hospitalization (COH) is ＄2,522.86. The main influencing factors include the following：① CT imaging: lesions of the lungs were bilateral and multiple. The range of involved and interstitial changes affect the medical burden significantly (P=0.009 and P=0.013, respectively); ②Treatments: Patients were more severe with high flow nasal cannula oxygen therapy (HFNC) or used methylprednisolone more than 1mg / kg; ③ Blood test indicators: lymphocyte (LC) count level Conclusions: The hospitalization cost of non-severe COVID-19 is higher than that of the common CAP patients. The main influencing factors are multiple extensive interstitial changes on CT scans, absolute lymphocyte level

To explore the water purification efficiency and phytoplankton control efficiency of the water source ecological purification system, and evaluate the effectiveness of the functional group (FG) and morphology-based functional group (MBFG) in response to the internal environment and water quality of the water source ecological purification system, in the summer of 2018, the water quality and phytoplankton functional groups of each unit of the Yanlong Lake ecological purification system were monitored and analyzed. The results showed that the Yanlong Lake water source ecological purification system can effectively purify the water. The average values of total phosphorus, total nitrogen, dissolved oxygen, and turbidity in the influent water were 0.20 mg·L

The isothermal constant strain rate compression test of Ti-10.2Mo-4.9Zr-5.5Sn alloy was carried out by Gleeble-3500 thermomechanical simulator. The hot deformation behavior at temperature of 670–820 °C and strain rate of 0.001–10s−1 was studied, and the activation energy of hot deformation was calculated. The results show that the flow stress of the alloy is more sensitive to the deformation temperature and strain rate, and is reduced by the increase of the deformation temperature and the decrease of the strain rate; the activation energy of the hot deformation of the alloy is higher than the self-diffusion activation energy of pure α titanium and pure β titanium alloy. According to the error calculation, the correlation coefficient and the average relative error of the constitutive model of Ti-10.2Mo-4.9Zr-5.5Sn alloy established by strain compensation are 0.9716 and 6.24%, and they own good precision. Based on the hot processing map of the Ti-10.2Mo-4.9Zr-5.5Sn alloy set up by the dynamic material model, instability zone's unstable form is mainly local flow and stable zone's deformation mechanism is mainly dynamic recovery and dynamic recrystallization through analyzing stable and unstable zones' microstructure; and it is found that the suitable deformation parameters of Ti-10.2Mo-4.9Zr-5.5Sn alloy are as follows: deformation temperature 670–700 °C, strain rate 0.001–0.003s−1, deformation temperature 790–820 °C, and strain rate 0.001–0.1s−1 through the processing map and microstructure observation.

Solar heating system has raised much attention due to the global energy shortage and environment pollution. The dynamic heat release performance of water tank (WT) with phase change materials (PCM) plays an important role in the operation of solar heating system, while seldom available literatures focused on this research topic. In this paper, an experimental system for heat release of the WT-PCM was established. The temperature variations of the water and the PCM during heat release of the WT-PCM and the traditional water tank were measured respectively, and their dynamic heat release performance were also analyzed based on the measured data. The results are as follows: the heat release of the WT-PCM can be divided into three phases: sensible heat before phase change, latent heat during phase change and sensible heat after phase change, and the water temperature will be temporarily heated after the sudden drop; the water supply temperature decline rate of both tanks will increase dramatically at the initial stage of heat release, when the PCM temperature is reduced to 50℃, the water supply decline rate of the WT-PCM is less than that of the traditional water tank, while when PCM is completely solidified, the water supply temperature decline rate of the WT-PCM is more than that of the traditional water tank. The heat release performance of the WT-PCM is much better than that of the traditional water tank. The research results can provide useful reference for engineering application of the WT-PCM.

Our previous studies had identified that both crude extracts and total alkaloid from Chelidonium majus exerted a significant antifeeding and larval lethality on Lymantria dispar. Moreover, sanguinarine, chelidonine, berberine hydrochloride and coptisine were the main alkaloid in C. majus exerting toxicity to L. dispar. In this paper, we evaluated the insecticidal and antifeeding activities of each alkaloid on the 3rd instar L. dispar larvae by bioassay. Meanwhile, the effects of alkaloids from C. majus on the activities and mRNA levels of three main digestive enzymes in L. dispar larvae were investigated. The results indicated that sanguinarine possessed the strongest insecticidal activity with a LD50 value of 4.963 μg/larva, and the coptisine showed little lethality to 3 rd instar L. dispar larvae among four alkaloids from C. majus. The insecticidal capacity of four alkaloids on 3rd instar L. dispar larvae was in the following decreasing order of sanguinarine > chelidonine > berberine hydrochloride > coptisine. Similarly, except coptisine, the other three alkaloids significantly reduced food intakes of third instar L. dispar larvae and suppressed activities of three digestive enzymes (α-amylase, lipase and total protease) simultaneously. Finally, qRT-PCR analysis revealed that the transcriptions of α-amylase, lipase and serine protease were affected by sanguinarine. Especially, at 48 h after treatment, the mRNA expressions of those digestive enzymes were significantly suppressed by sanguinarine. In conclusion, we suggested that alkaloids from C. majus induced antifeeding and larval lethality on L. dispar larvae by suppressing food intake and digestive enzymes in L. dispar. Our findings provide a novel insight into evaluating the antifeeding and insecticidal properties of C. majus, which afford a new strategy for integrated pest management programs as well.

In this article, the authors investigate the complete convergence and complete moment convergence of the maximum partial sums for arrays of rowwise asymptotically almost negatively associated random variables without assumptions of identical distribution and stochastic domination, and obtain some new results, which not only generalize the corresponding theorems of Hu and Taylor (1997), Gan and Chen (2007),Wu (2012), but also improve them, respectively.

Millilensing of Gamma-Ray Bursts (GRBs) is expected to manifest as multiple emission episodes in a single triggered GRB with similar light-curve patterns and similar spectrum properties. Identifying such lensed GRBs could help improve constraints on the abundance of compact dark matter. Here we present a systemic search for millilensing among 3000 GRBs observed by the \textit{Fermi} GBM up to 2021 April. Eventually we find 4 interesting candidates by performing auto-correlation test, hardness test, and time-integrated/resolved spectrum test to the whole sample. GRB 081126A and GRB 090717A are ranked as the first class candidate based on their excellent performance both in temporal and spectrum analysis. GRB 081122A and GRB 110517B are ranked as the second class candidates (suspected candidates), mainly because their two emission episodes show clear deviations in part of the time-resolved spectrum or in the time-integrated spectrum. Considering a point mass model for the gravitational lens, our results suggest that the density parameter of lens objects with mass $M_{\rm L}\sim10^{6} M_{\odot}$ is larger than $1.5\times10^{-3}$., Comment: Accepted for publication in The Astrophysical Journal

In this paper, we consider the complete convergence for weighted sums of negatively superadditive-dependent (NSD) random variables without assumptions of identical distribution. Some suffic...

The enzymatic antioxidant system (EAS) protects aerobic cells from oxidative stress. However, it is brittle and susceptible of inactivation of reactive oxygen species (ROS) immoderate production. Here, we demonstrated that MoS2 nanosheets (few-layer MoS2), as a multifunctional nanozyme, possess intrinsic activity of mimicking enzymes of superoxide dismutases (SODs), catalases (CATs), and peroxidases (PODs) under physiological conditions (pH 7.4, 25 °C). Further, MoS2 nanosheets showed POD-like activity by transferring electrons instead of generating ROS. Similar to EAS, a defense termed nanozymatic antioxidant system (NAS) was developed by MoS2 nanosheets, for regulation of oxidative stress. Surprisingly, this NAS can effectively scavenge other free radicals including hydroxyl radicals (•OH), nitrogen-centered free radicals (•DPPH), and nitric oxide (•NO). To evaluate these unique properties of MoS2-based NAS in vivo, Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and A549 cell models were...

A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D-π-A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.

The increasing impact of bacteria on cancer progression and treatments has been witnessed in recent years. Insufficient attention to cancer-related bacteria may lead to distant metastasis, poor therapeutic efficiency and low survival rates for cancers. Exploiting new approaches that enable selective imaging and effective killing of cancer cells and bacteria are thus of great value for the battle against cancers. Herein, we report an aggregation-induced emission (AIE) luminogen, namely TPPCN, with intense emission and efficient reactive oxygen species production for fluorescence imaging and killing cancer cells and Gram-positive bacteria. This work not only demonstrates the potential of AIE luminogens in comprehensive cancer treatments but also stimulates the enthusiasm of scientists to design more multifunctional AIE systems for both cancer and bacteria theranostics.

The study of the gravity response of roots and shoots is of great significance when exploring the polarity of plants and the development of the forest industry. In our study, normal and inverted cuts of Salix matsudana (Koidz) were cultured. The total RNAs of roots and shoots were extracted. Based on the comparative transcriptome, 412 and 668 genes were differentially expressed. The plasma membrane-, cell wall-, and extracellular region-related genes were up-regulated in the shoots, while the carbon metabolism and the nitrogen metabolism were up-regulated in the roots. Combining the alternative splicing genes, we found a potential gravity response network: in the shoots, LecRLKs were highly sensitive to gravity and further affected the alternative splicing of SNARE, as well as inducing an inhomogeneous distribution of auxin and a negative geotropism curve. In the roots, AP2/ERFs and STRKs were highly sensitive to gravity and regulated the expression level of STPKs and WAKs, finally resulting in a geotropism curve. Moreover, cell division was suppressed in both the roots and the shoots under inverted conditions with different mechanisms. Cell division inhibitors (KRPs) were up-regulated in the roots, while DNA helicase MCMs were down-regulated in the shoots. These results provide an important foundation for further studies of the molecular mechanisms and genetic regulation of plant responses to gravity and the plant polarity of forest trees.

The utilization of renewable biomass cellulose in treating pollution of heavy metal ions (HMIs) is one of overarching and appealing strategies, because it simultaneously satisfies sustainable development and resolves ever-increasing environmental issue. In this regard, the composite cellulose@Mg(OH)2 was prepared via a facile method and explored for its use as water treatment agent. It is demonstrated that the smaller and thinner hexagonal Mg(OH)2 flakes are constructed onto the cellulose substrate by self-assembling; two components are chemically coupled via hydrogen bonds and Mg-Oc (cellulose oxygen) dative bonds; what’s more, the coupling of cellulose with Mg(OH)2 (1 0 1) facet is much more preferential than with (0 0 1) facet. The resultant composite material shows remarkable HMI removal performance: large capacities of 734.9, 595.8 and 1473.1 mg g−1 for Cd2+, Cu2+ and Pb2+, respectively. Further assisted by good shaping property of the cellulose substrate, the composite is capable of being made into filter, which practically separates HMIs and purifies wastewater with high removal efficiency (99.99%) for Cd2+ even after operating for 110 days and potable water can be obtained. The mechanism is delineated with removal models and characterizations of HMI-recovered products.

In this paper, we set up a solar energy-phase change heat storage system and experimental study on the matching of the phase-change tank and three different terminal cooling equipment. Among them, phase-change water tank is used as heat source, meanwhile, radiator, heater and capillary as terminal cooling equipment. When radiator the terminal is radiator, the indoor temperature is difficult to meet the heating temperature requirements. When the terminal is heater, the indoor temperature can meet the requirements of heating temperature. However, the longitudinal distribution and lateral distribution of indoor air temperature are unevenly distributed and affect the thermal comfort of human body. When the terminal is capillary tube, the indoor temperature maintain at a high temperature and the temperature is satisfied with the thermal comfort of human body. Therefore, the capillary tube as the terminal cooling equipment is more advantageous of matching with the phase-change tank.

Giant flares (GFs) are unusual bursts from soft gamma-ray repeaters (SGRs) that release an enormous amount of energy in a fraction of a second. The afterglow emission of these SGR-GFs or GF candidates is a highly beneficial means of discerning their composition, relativistic speed, and emission mechanisms. GRB 200415A is a recent GF candidate observed in a direction coincident with the nearby Sculptor galaxy at 3.5 Mpc. In this work, we searched for transient gamma-ray emission in past observations by Fermi-LAT in the direction of GRB 200415A. These observations confirm that GRB 200415A is observed as a transient GeV source only once. A pure pair-plasma fireball cannot provide the required energy for the interpretation of GeV afterglow emission and a baryonic poor outflow is additionally needed to explain the afterglow emission. A baryonic rich outflow is also viable, as it can explain the variability and observed quasi-thermal spectrum of the prompt emission if dissipation is happening below the photosphere via internal shocks. Using the peak energy ($E_p$) of the time-resolved prompt emission spectra and their fluxes ($F_p$), we found correlation between $E_p$ and $F_p$ or $E_p$ and isotropic luminosity $L_{\rm iso}$ for GRB 200415A. This supports the intrinsic nature of $E_p$-$E_{\rm iso}$ correlation found in SGRs-GFs, hence favoring a baryonic poor outflow. Our results also indicate a different mechanism at work during the initial spike, and that the evolution of the prompt emission spectral properties in this outflow would be intrinsically due to the injection process., Accepted for publication in RAA

To explore the interactions of nanoparticles and bioresources and elucidate their effects on the morphology of the resulting composite, hierarchically structured cellulose@ZnO composites have been synthesized by an environmentally friendly hydrothermal method in one step. First, self-assembly induces the formation of hierarchical three-level structures, including cellulose/ZnO nanofibers, layers, and microfibers. Then, ZnO microparticles deposit onto the surface of the third-level cellulose/ZnO microfibers and accomplish the fabrication of a cellulose@ZnO composite, which eventually defines the hierarchical morphology of synthesized materials. The self-assembly mechanism was comprehensively examined. The electrostatic attraction between cellulose and ZnO, not hydrogen bonding, was found to be the main driving force for the formation of the first-level structure. A density functional theory study was conducted to support the self-assembly mechanism by optimizing the cellulose/ZnO structures at the molecula...

This study aimed to explore the effects of RACK1 gene silencing on the apoptosis and proliferation of hepatocellular carcinoma (HCC) MHCC97-H cells. After transfecting MHCC97-H cells with siRNA, RACK1 gene silencing model was established. The cells were divided into blank group, siRNA group and empty plasmid group, respectively. The mRNA and protein expressions of RACK1, cyclin D1 and BAX were determined by qRT-PCR and Western blotting. CCK-8 assay, flow cytometry and FITC-Annexin V/PI staining were used to determine cell viability, cell cycle and cell apoptosis, respectively. The results of qRT-PCR and Western blotting suggested that when compared with the blank group and the empty plasmid group, the mRNA and protein expressions of RACK1 and Cyclin D1 decreased significantly while the mRNA and protein BAX expressions increased substantially in the siRNA group (all P

GRB 200219A is a short gamma-ray burst (GRB) with an extended emission (EE) lasting $\sim 90$s. By analyzing data observed with the {\em Swift}/BAT and {\em Fermi}/GBM, we find that a cutoff power-law model can adequately fit the spectra of the initial short pulse with $\rm E_{p}=1387^{+232}_{-134}$ keV. More interestingly, together with the EE component and early X-ray data, it exhibits plateau emission smoothly connected with a $\sim t^{-1}$ segment and followed by an extremely steep decay. The short GRB composed of those three segments is unique in the {\em Swift} era and is very difficult to explain with the standard internal/external shock model of a black hole central engine, but could be consistent with the prediction of a magnetar central engine from the merger of an NS binary. We suggest that the plateau emission followed by a $\sim t^{-1}$ decay phase is powered by the spin-down of a millisecond magnetar, which loses its rotation energy via GW quadrupole radiation. Then, the abrupt drop decay is caused by the magnetar collapsing into a black hole before switching to EM-dominated emission. This is the first short GRB for which the X-ray emission has such an intriguing feature powered by a magnetar via GW-dominated radiation. If this is the case, one can estimate the physical parameters of a magnetar, the GW signal powered by a magnetar and the merger-nova emission are also discussed., Comment: 21 pages, 2 Tables, 7 figures, ApJL in press, two more references added, matched the published version

The giant flares of soft gamma-ray repeaters (SGRs) have long been proposed to contribute to at least a subsample of the observed short gamma-ray bursts (GRBs). In this paper, we perform a comprehensive analysis of the high-energy data of the recent bright short GRB 200415A, which was located close to the Sculptor galaxy. Our results suggest that a magnetar giant flare provides the most natural explanation for most observational properties of GRB 200415A, including its location, temporal and spectral features, energy, statistical correlations, and high-energy emissions. On the other hand, the compact star merger GRB model is found to have difficulty reproducing such an event in a nearby distance. Future detections and follow-up observations of similar events are essential to firmly establish the connection between SGR giant flares and a subsample of nearby short GRBs., Comment: ApJ published in August 2020 (Yang et al. 2020, ApJ, 899, 106)

Mine ecological restoration is the current necessary step in the process of resources exploitation, so for the study of the mining area ecological restoration, ecological restoration of phosphate rock mountain lands after the ecological benefit evaluation, using the analytic hierarchy process (AHP) method and frequency analysis method to select the phosphate rock mountain land ecological benefit evaluation index, evaluated by calculation results shows that the calculation by using the underground runoff water conservation quantity is 11832.036 m3/a with water and soil conservation quantity of 14976.4 m3/a. If the area of vegetation broad-leaved forest that can be formed after ecological restoration in the mining area is 50.0 h m2 and the area of coniferous forest is 10.0 h m2, the blocking dust capacity can be obtained to be 832.11 t/a. The indirect economic value of water conservation, water and soil conservation, and environmental purification calculated by shadow engineering method is respectively 8791.6 yuan/a, 166.6 yuan/a, and 141458.7 yuan/a, and the environmental value of water purification is 13121.784 yuan/a, and the overall indirect economic benefit value is 1.64 105 yuan/a. The practice shows that the research index of ecological restoration strategy is more comprehensive and scientific, which provides another method for ecological environment restoration strategy of mining areas in China and promotes the sustainable development of ecological environment.

Recently two fast X-ray transients (XT1 and XT2) have been reported from the search in the Chandra Deep Field (CDF) data. Each transient shows an initial plateau lasting around hundreds to thousands seconds followed by a rapid decay in the light curve. In particular, CDF-S XT2 is found to be associated with a galaxy at redshift z= 0.738 and was explained as a counterpart of a binary neutron-star merger event. In this paper, motivated by the short duration and decay slopes of the two transients, we consider an alternative interpretation in which both events are accretion-driven flares from tidal disruption of white dwarfs by intermediate-mass black holes. We derive a theoretical model of the accretion rate history, and find that it fits the observed X-ray light curves well. The extremely super-Eddington peak luminosity of XT2 can be explained by the beaming effect of the system, likely in the form of a jet., 8 pages, 3 figures, 1 table. Accepted for publication in APJL

Assuming that the number of possible decisions for a transmitter (e.g., the number of possible beamforming vectors) has to be finite and is given, this paper investigates for the first time the problem of determining the best decision set when energy-efficiency maximization is pursued. We propose a framework to find a good (finite) decision set which induces a minimal performance loss w.r.t. to the continuous case. We exploit this framework for a scenario of energy-efficient MIMO communications in which transmit power and beamforming vectors have to be adapted jointly to the channel given under finite-rate feedback. To determine a good decision set we propose an algorithm which combines the approach of Invasive Weed Optimization (IWO) and an Evolutionary Algorithm (EA). We provide a numerical analysis which illustrates the benefits of our point of view. In particular, given a performance loss level, the feedback rate can by reduced by 2 when the transmit decision set has been designed properly by using our algorithm. The impact on energy-efficiency is also seen to be significant., 7 pages, 5 figures

In insects, 20-hydroxyecdysone (20E) mediates developmental transitions and regulates molting processes through activation of a series of transcription factors. Broad-Complex (Br-C), a vital gene in the 20E signalling pathway, plays crucial roles during insect growth processes. However, whether Br-C affects chitin synthesis in insects remains unclear. In the present study, the Br-C gene from Lymantria dispar, a notorious defoliator of forestry, was identified based on transcriptome data, and subjected to bioinformatic analysis. The regulatory functions of LdBr-C in chitin synthesis and metabolism in L. dispar larvae were analysed by RNA interference (RNAi). The full-length LdBr-C gene (1431 bp) encodes a 477 amino acid (aa) polypeptide containing a common BRcore region (391 aa) at the N-terminus and a C-terminal Zinc finger domain (56 aa) harbouring two characteristic C2H2 motifs (CXXC and HXXXXH). Phylogenetic analyses showed that LdBr-C shares highest homology and identity with Br-C isoform 7 (83.12%) of Helicoverpa armigera. Expression profiles indicate that LdBr-C was expressed throughout larval and pupal stages, and highly expressed in prepupal and pupal stages. Furthermore, LdBr-C expression was strongly induced by exogenous 20E, and suppressed dramatically after application of dsLdBr-C. Bioassay results showed that knockdown of LdBr-C caused larval developmental deformity, significant weight loss, and a mortality rate of 67.18%. Knockdown of LdBr-C significantly down-regulated transcription levels of eight critical genes (LdTre1, LdTre2, LdG6PI, LdUAP, LdCHS1, LdCHS2, LdTPS and LdCHT) related to chitin synthesis and metabolism, thereby lowering the chitin content in the midgut and epidermis. Our findings demonstrate that Br-C knockdown impairs larval development and chitin synthesis in L. dispar.

Background: Thoracogastric-airway fistula (TGAF) post-thoracic surgery is a rare and challenging complication for esophagectomy. The aim of this study was to explore the effectiveness of airway stenting for TGAF patients and find related factors coupled with healing of fistula. Methods: This is a retrospective study involving patients with TGAF who were treated with airway stentings. Based on different TGAF locations and sizes on chest computed tomography, covered metallic or silicon airway stents were implanted to cover orifices under interventional bronchoscopy. TGAF healing was defined as the primary outcome, and complete sealing of TGAF as the second outcome. The predictors for TGAF healing were analyzed in univariate and multivariate analysis. Results: A total of 58 TGAF patients were included, of whom 7 received straight covered metallic stents, 5 straight silicon stents, 3 L-shaped covered metallic stents, 21 large Y-shaped covered metallic stents, 17 large Y-shaped silicon stents, and 5 with Y-shaped covered metallic stents. Healing was achieved in 20 (34.5%) patients, and complete sealing in 45 (77.6%) patients. There were no significant differences in healing rate and complete sealing rate between patients receiving metallic stents and those with silicon stents. In univariate analysis, lacking a previous history of radiotherapy or chemotherapy, nonmalignant fistulas, small fistulas, and shorter postesophagectomy duration were found associated with a higher rate of TGAF healing. Only shorter postesophagectomy duration was associated with TGAF healing in multivariate analysis. Conclusions: Both silicon and covered metallic airway stenting are effective methods to close TGAF. A shorter postesophagectomy period may predict better TGAF healing. The reviews of this paper are available via the supplemental material section.

At present, there is still an urgent demand for novel smart materials that can achieve a diverse range of practical applications in synthetic material area. Herein, we developed a simple but versatile aggregation-induced emission luminogen (1) with a donor-acceptor structure and pronounced intramolecular charge transfer property. Compound 1 showed a remarkable color change in sensitive response to polarity change making it to serve as a promising imaging probe for detecting environmental polarity in cells and selective visualization of lipid droplets in live tissues. Additionally, this compound exhibited a wide range of thermoresponsive behavior with ratiometric luminescence change and noticeable fluorescence color switching. It also can respond to anisotropic shearing force and isotropic hydrostatic pressure with prominent but contrast luminescence conversion due to the distinct disturbance of the weak intermolecular interactions and charge transfer processes. Meanwhile, compound 1 was sensitive to external electric stimulus and displayed reversibly three-color switched electrochromism and on-to-off electroluminochromism. Such property allowed the fabrication of high-performance non-doped OLED with a high external quantum efficiency of 5.22%. The present results may offer an important guideline for multifunctional molecular design and provide an important step forward to expand the real-life applications of AIE-active smart materials.

Efficient organic photosensitizers (PSs) have attracted much attention because of their promising applications in photodynamic therapy (PDT). However, guidelines on their molecular design are rarely reported. In this work, a series of PSs are designed and synthesized based on a triphenylamine-azafluorenone core. Their structure-property-application relationships are systematically studied. Cationization is an effective strategy to enhance the PDT efficiency of PSs by targeting mitochondria. From the molecularly dispersed state to the aggregate state, the fluorescence and the reactive oxygen species generation efficiency of PSs with aggregation-induced emission (AIE) increase due to the restriction of the intramolecular motions and enhancement of intersystem crossing. Cationized mitochondrion-targeting PSs show higher PDT efficiency than that of nonionized ones targeting lipid droplets. The ability of AIE PSs to kill cancer cells can be further enhanced by combination of PDT with radiotherapy. Such results should trigger research enthusiasm for designing and synthesizing AIE PSs with better PDT efficiency and properties.

Hydrogen sulfide (H2 S) is emerging as an important signalling molecule that regulates plant growth and abiotic stress responses. However, the roles of H2 S in symbiotic nitrogen (N) assimilation and remobilization have not been characterized. Therefore, we examined how H2 S influences the soybean (Glycine max)/rhizobia interaction in terms of symbiotic N fixation and mobilization during N deficiency-induced senescence. H2 S enhanced biomass accumulation and delayed leaf senescence through effects on nodule numbers, leaf chlorophyll contents, leaf N resorption efficiency, and the N contents in different tissues. Moreover, grain numbers and yield were regulated by H2 S and rhizobia, together with N accumulation in the organs, and N use efficiency. The synergistic effects of H2 S and rhizobia were also demonstrated by effects on the enzyme activities, protein abundances, and gene expressions associated with N metabolism, and senescence-associated genes (SAGs) expression in soybeans grown under conditions of N deficiency. Taken together, these results show that H2 S and rhizobia accelerate N assimilation and remobilization by regulation of the expression of SAGs during N deficiency-induced senescence. Thus, H2 S enhances the vegetative and reproductive growth of soybean, presumably through interactions with rhizobia under conditions of N deficiency.