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821 results on '"Hakomori, Sen-itiroh"'

159. PARTICIPANTS

Author

Aminoff, David, primary, Beckman, Lars, additional, Brewer, George J., additional, Carlson, Don M., additional, Ceppellini, Ruggero, additional, Chuba, Joseph V., additional, Clauser, H., additional, Côté, R.-H., additional, Allen, D., additional, Davies, L., additional, Dunstone, J.R., additional, Furukawa, Ken, additional, Garza, Juan, additional, Gershowitz, Henry, additional, Gibbons, R.A., additional, Ginsburg, V., additional, Hakomori, Sen-itiroh, additional, Horowitz, Martin I., additional, Huprikar, Shankar V., additional, Iseki, Shoei, additional, Jamieson, G.A., additional, Jeanloz, Roger W., additional, Johnson, Arthur G., additional, Kabat, Elvin A., additional, Kościelak, J., additional, Lauf, P.K., additional, Lisowska, Elwira, additional, Lundblad, Arne, additional, McGuire, Edward J., additional, Mora, Peter T., additional, Morgan, W.T.J., additional, Neel, James V., additional, Ohkuma, Seiichi, additional, Pigman, Ward, additional, Poulik, M.D., additional, Radin, Norman S., additional, Rasmusen, Benjamin A., additional, Sanger, Ruth, additional, Saunders, A.M., additional, Schachter, Harry, additional, Shreffler, Donald C., additional, Springer, Georg F., additional, Szulman, A.E., additional, Uhlenbruck, G., additional, Watkins, Winifred M., additional, Weiss, Michael, additional, Wiener, Alexander S., additional, and Winzler, Richard J., additional

Published
1970
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168. Phenotypic changes in 3T3 cells associated with the change of sphingolipid synthesis by a ceramide analog, 2-decanoylamino-3-morpholino-1-phenylpropanol (compound RV538)

Author

Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA, Program of Biochemical Oncology, Fred Hutchinson Cancer Research Center and Departments of Pathobiology, Microbiology and Immunology, University of Washington, Seattle, WA 98104, USA, Okada, Yoshio, Radin, Norman S., Hakomori, Sen-itiroh, Mental Health Research Institute, University of Michigan, Ann Arbor, MI 48109, USA, Program of Biochemical Oncology, Fred Hutchinson Cancer Research Center and Departments of Pathobiology, Microbiology and Immunology, University of Washington, Seattle, WA 98104, USA, Okada, Yoshio, Radin, Norman S., and Hakomori, Sen-itiroh

Abstract

A culture of BALB/c 3T3 cells grown in the presence of 40 [mu]M of the ceramide analog compound RV538 (2-decanoylamino-3-morpholino-1-phenylpropanol) for several passages caused a substantial decrease in the level of all glycosphingolipids and an accumulation of ceramide and sphingomyelin. Associated with these chemical changes of sphingolipid composition and metabolism, the following phenotypic changes were observed: (i) loss of the cobblestone appearance at cell density saturation and development of fibroblastic appearance with partial overlapping of cells; (ii) reduction of cell growth rate; (iii) enhanced production of lactic acid; (iv) enhanced rate of glucose transport; and (v) higher incidence of large colony formation with infiltrating appearance in soft agar. Cell morphology changes, lactate pruduction, and enhanced sugar uptake were reversed by co-culturing cells with gangliosides, particularly trisialogangliosides. Thus, these phenotypic changes mimicking those of oncogenically transformed cells are closely related to the blocked synthesis of glycolipids in these cells, whereas other changes may be caused by an accumulation of ceramide and sphingomyelin.

Published
2006

172. Evaluation of ricin A chain-containing immunotoxins directed against glycolipid and glycoprotein on mouse lymphoma cells

Author

Usui, Hiroko and Hakomori, Sen-Itiroh

Subjects
glycoprotein, endocrine system, Lymphoma, viruses, Immunotoxins, Ricin, biochemical phenomena, metabolism, and nutrition, Glycosphingolipids, immunotoxin, Neoplasm Proteins, carbohydrates (lipids), Antigen-Antibody Reactions, Mice, Evaluation Studies as Topic, Gangliosides, Biomarkers, Tumor, Tumor Cells, Cultured, Animals, lipids (amino acids, peptides, and proteins), glycolipid, Glycoproteins
Abstract

Immunotoxins composed of monoclonal antibodies (mAbs) and various toxins have been developed for the treatment of malignancies. We investigated the efficacy of three ricin toxin A-chain (RTA)-containing immunotoxins (ITs) conjugated from mAbs which recognize glycolipid asialo-GM2 and glycoprotein H-2d. These ITs retained the same immunoreactivity with mAbs. We evaluated the cytotoxicity of these ITs against mouse lymphoma cells L5178Y variants showing high (AA12,CC9) and low (27AV) expression of asialo-GM2. Anti-H-2d-RTA IT had the strongest cytotoxicity for all cell lines. Anti-asialo-GM2 (IgM)-RTA IT had stronger cytotoxicity than anti-asialo-GM2 (IgG3)-RTA IT. Anti-asialo-GM2-RTA ITs had different cytotoxicity against AA12 and CC9 cells. The establishment of appropriate anti-glycolipid mAbs may lead to effective immunotargeting therapy.

Published
1994

177. A Novel Ganglioside Isolated from Renal Cell Carcinoma

Author

Ito, Akihiro, Levery, Steven B., Saito, Seiichi, Satoh, Makoto, Hakomori, Sen-itiroh, Ito, Akihiro, Levery, Steven B., Saito, Seiichi, Satoh, Makoto, and Hakomori, Sen-itiroh

Abstract

In renal cell carcinoma (RCC), the level of higher gangliosides is correlated with degree of metastatic potential, and cell lines derived from metastatic deposits of RCC are characterized by high expression of disialogangliosides (Saito, S., Orikasa, S., Ohyama, C., Satoh, M., and Fukushi, Y. (1991) Int. J. Cancer 49, 329–334 and Saito, S., Orikasa, S., Satoh, M., Ohyama, C., Ito, A., and Takahashi, T. (1997) Jpn. J. Cancer Res. (Gann) 88, 652–659). We now report two disialogangliosides, G1 and G2, found in the RCC cell line TOS-1. G1 from TOS-1 cells was characterized as having a novel hybrid structure between ganglio-series (region I as in Structure FTI; same as the terminal structure of ganglioside GM2), and the lacto-series type 1 (region II). The characterization was based on reactivity with various monoclonal antibodies (mAbs) with defined epitope specificity, as well as monosaccharide and fatty acid component analysis, ^1H NMR spectroscopy, and electrospray ionization mass spectrometry of the intact compound. G1 showed strong reactivity with mAb RM2, raised originally against TOS-1 cells, and weak cross-reactivity with anti-GM2 mAb MK-1–8. The antigen is hereby termed GalNAc disialosyl Lc_4Cer (IV^4GalNAcIV^3NeuAcIII^6NeuAcLc_4; abbreviated GalNAcDSLc_4). G2 was identified by^1H NMR and mass spectrometry as having a structure similar to Structure FTI but without the GalNAcβ1→4 substitution and showed strong reactivity with mAb FH9 reported previously to be specific for disialosyl lacto-series type 1 (disialosyl Lc_4) having vicinal α2→3 and α2→6 sialosyl residues, an antigen associated with human colonic cancer. Clinicopathological studies indicate that expression of these disialoganglioside antigens in RCC tissue is correlated with the metastatic potential of RCC.

Published
2001

183. Glycosylation status of haptoglobin in sera of patients with prostate cancervs. benign prostate disease or normal subjects

Author

Fujimura, Tsutomu, primary, Shinohara, Yasuro, additional, Tissot, Bérangère, additional, Pang, Poh-Choo, additional, Kurogochi, Masaki, additional, Saito, Seiichi, additional, Arai, Yoichi, additional, Sadilek, Martin, additional, Murayama, Kimie, additional, Dell, Anne, additional, Nishimura, Shin-Ichiro, additional, and Hakomori, Sen-itiroh, additional

Published
2007
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