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Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

Engineering of enzymes on the basis of protein structures are rational and efficient approaches to acquire biocatalysts of desired performances. In this study, we focused on a special mono- and diacylglycerol lipase (MDGL) isolated from the lipolytic enzyme-enriched fungus Aspergillus oryzae and discovered improved variants based on its crystal structure. We first solved the crystal structure of Aspergillus oryzae lipase (AOL) at 1.7 A resolution. Structure analysis and sequence alignment of AOL and other MDGLs revealed that the residue V269 is of vital importance for catalysis. Replacement of the V269 in AOL with the corresponding residues in other MDGLs has led to noticeable changes in hydrolysis without sacrificing the thermostability and substrate specificity. Among the investigated variants, V269D exhibited about a six-fold higher hydrolysis activity compared to the wild type. Molecular dynamics simulations and protein-ligand interaction frequency analyses revealed that the Asp substitution enhanced the substrate affinity of AOL. Our work sheds light on understanding the catalytic process of AOL and helps tailoring MDGLs with desired catalytic performance to fulfill the demand for biotechnological applications.

In this work, the bio-based epoxy nanocomposites (GER/DOPO-POSS) were prepared, and AE-POSS was synthesized and used as compatibilizer for GER/DOPO-POSS nanocomposites. The effects of AE-POSS content on mechanical properties, thermal stability, compatibility and flame retardancy of GER/DOPO-POSS nanocomposites were discussed. The results showed that the addition of AE-POSS significantly improved the dispersity of DOPO-POSS in gallic epoxy matrix. AE-POSS can improve both the thermal stability and flame retardancy of GER/DOPO-POSS nanocomposites. When 6.0 wt % AE-POSS was added in GER/DOPO-POSS, the limiting oxygen index of it was 25.6%, and the initial thermal degradation temperature of it was 36 °C higher than that without AE-POSS.

Pre-eclampsia (PE) is a leading cause of maternal and fetal morbidity and mortality. Our aim was to assess epicardial adipose tissue (EAT) thickness as a potential effective screening parameter to predict PE in the first trimester.133 women with PE were enrolled in this study, and divided into the early-onset and late-onset subgroups. 150 healthy women were enrolled as control group. Anthropometric and echocardiographic parameters were obtained, including EAT thickness. Generalized linear models was used to assess the predict efficiency of EAT with fdds ratio (OR) and 95% confidence intervals (CIs).Compared to the controls, EAT thickness was higher in PE patients (P 0.05). Multivariate regression analysis indicated that EAT thickness was associated with PE risk (OR = 1.50, 95%CI: 1.19-1.88, P 0.05). The area under curves of EAT thickness was 0.616. When the cutoff value for EAT thickness was set to 5.26 mm, the sensitivity was 78.2% and the specificity was 44.7%. EAT thickness significantly both higher in the early-onset and late-onset groups compared to the controls; Meanwhile, EAT thickness was higher in the early-onset group than late-onset group (P 0.05). Logistic regressionrevealed the close relationship between onset time of PE and EAT thickness (OR = 1.59, 95%CI: 1.28-1.98, P 0.001).Measured EAT thickness using echocardiography could identify high-risk people for PE as early as the first trimester. Echocardiographic EAT has the potential to be a predictor for PE prior to actual clinical diagnosis.

Nisin is commonly used as a biopreservative in foods. For industrial production, nisin-producing Lactococcus lactis strains are usually grown to high cell densities to achieve the highest possible nisin titer. However, accumulation of lactic acid eventually halts production, even in pH-controlled fermentations. Here, we describe a nisin-producing L. lactis strain Ge001, which was obtained after transferring the nisin gene cluster from L. lactis ATCC 11454, by conjugation, into the natural mutant L. lactis RD1M5, with low lactate dehydrogenase activity. The ability of Ge001 to produce nisin was tested using dairy waste as the fermentation substrate. To accommodate redox cofactor regeneration, respiration conditions were used, and to alleviate oxidative stress and to reduce adsorption of nisin onto the producing cells, we found it to be beneficial to add 1 mM Mn2+ and 100 mM Ca2+, respectively. A high titer of 12 084 IU/mL nisin could be reached, which is comparable to the highest titers reported using expensive, rich media. Summing up, we here present a 100% natural, robust, and sustainable approach for producing food-grade nisin and acetoin from readily available dairy waste.

Exercise has been recommended as an important strategy to improve glucose metabolism in obesity. Adipose tissue fibrosis is associated with inflammation and is implicated in glucose metabolism disturbance and insulin resistance in obesity. However, the effect of exercise on the progression of adipose tissue fibrosis is still unknown. The aim of the present study was to investigate whether exercise retarded the progression of adipose tissue fibrosis and ameliorated glucose homeostasis in diet-induced obese mice. To do so, obesity and adipose tissue fibrosis in mice were induced by high-fat diet feeding for 12 weeks and the mice subsequently received high-fat diet and exercise intervention for another 12 weeks. Exercise alleviated high-fat diet-induced glucose intolerance and insulin resistance. Continued high-fat diet feeding exacerbated collagen deposition and further increased fibrosis-related gene expression in adipose tissue. Exercise attenuated or reversed these changes. Additionally, PPARγ, which has been shown to inhibit adipose tissue fibrosis, was observed to be increased following exercise. Moreover, exercise decreased the expression of HIF-1α in adipose fibrosis, and adipose tissue inflammation was inhibited. In conclusion, our data indicate that exercise attenuates and even reverses the progression of adipose tissue fibrosis, providing a plausible mechanism for its beneficial effects on glucose metabolism in obesity.

Heated tobacco products and electronic cigarettes are considered as alternatives to traditional tobacco cigarettes. However, it is crucial to monitor and compare the nicotine concentration in inhaled aerosols from these tobacco products, owing to the addictive nature and adverse effects of nicotine on human health. This study aimed to provide an electromembrane extraction (EME) combined liquid chromatography method to extract and determine nicotine in different inhaled aerosols. EME showed high extraction efficiency, selectivity, and sample clean-up capability. Under the optimal parameters, the linear range for nicotine was 0.1-200 mg L

Carbon emission reduction has been a consensus goal for most countries to achieve environmental sustainability. The use of carbon emission trading policies has been generally considered by the governments. Remanufacturing, as an effective way to reduce carbon emission, is incorporated together with the tool of carbon emission policy to construct a low-carbon supply chain in this paper. We analyze the carbon emission reduction and profit maximization problem among enterprises of original equipment manufacturers (OEMs) and their outsourcing remanufacturers, integrating the impact of the carbon emission constraint policy and the carbon market. Considering consumer preferences on low-carbon products and recycling rates of waste products, we construct a Stackelberg game model (dominated by the OEM) and analyze the impact of a carbon emission constraint policy on sales price, volume, carbon emission, and revenue of new and remanufactured products in the supply chain system. The results suggest that the upper bound set by the government on carbon emission for enterprises positively affects sales volume of new products and negatively affects sales prices of both products. Moreover, the discount rate of carbon emission constraint negatively affects sales volume of new products and positively affects sales prices of both products. Notably, the carbon emission constraint policy has impacts on the production decisions of both manufacturers on an economic scale. When the upper bound of carbon emission is equal to a certain threshold, the OEM could obtain the greatest revenue. The results provide a new perspective for the government to attain the goal of carbon emission reduction and not sacrifice economic growth. Managers in outsourcing remanufacturers and OEMs could also be implicated from our results to collaborate in allocating remanufacturing orders to achieve win-win opportunities between them.

OBJECTIVE: The Nuss procedure for pectus excavatum involves the risk of cardiopulmonary injury during the creation of retrosternal tunnel. This study describes the enlargement of retrosternal tunnel during Nuss procedure to prevent such problems caused by the inability to directly visualize the left pleural cavity.METHODS: From January 2018 to August 2021, 132 patients with pectus excavatum who underwent the Nuss procedure were divided into the modified group and the conventional group , according to the surgical method performed. The preoperative, intraoperative and postoperative indicators of the two groups were compared. In 70 consecutive patients undergoing the modified Nuss procedure, the separation was expanded by inserting the introducer bluntly and creating a retrosternal tunnel width of 4-5 cm in a coronal horizontal position. Once the pericardium, left parietal pleura and lung had been observed from the right thoracic cavity during the separation, the parietal pleura was punctured to induce a left pneumothorax and left lung collapse. Under thoracoscopic guidance, the introducer was passed through the left intercostal space.RESULTS: All children completed the surgery successfully; there were no cases of death, pericardial or cardiopulmonary injury, or bar displacement. There was no statistical significance in other indicators except age between two groups.CONCLUSION: Coronal plane enlargement of the retrosternal tunnel during the Nuss procedure allows direct visualization of the pericardium, left parietal pleura, and left lung, which has the promise of reducing the incidence of serious complications such as intraoperative cardiopulmonary injury and does not increase the incidence of general complications.

This review summarizes the anti-depressant mechanisms of repetitive transcranial magnetic stimulation in preclinical studies, including anti-inflammatory effects mediated by activation of nuclear factor-E2-related factor 2 signaling pathway, anti-oxidative stress effects, enhancement of synaptic plasticity and neurogenesis via activation of the endocannabinoid system and brain derived neurotrophic factor signaling pathway, increasing the content of monoamine neurotransmitters via inhibition of Sirtuin 1/monoamine oxidase A signaling pathway, and reducing the activity of the hypothalamic-pituitary-adrenocortical axis. We also discuss the shortcomings of transcranial magnetic stimulation in preclinical studies such as inaccurate positioning, shallow depth of stimulation, and difficulty in elucidating the neural circuit mechanism up- and down-stream of the stimulation target brain region.

Diabetes-associated cognitive impairment (DACI) can increase the risk of major cardiovascular events and death. Neuronal functionality is highly dependent on mitochondria and emerging evidence has shown that mitochondrial transplantation is a potential and effective strategy that can reduce brain injury and associated disorders. Platelets are abundant in blood and can be considered a readily available source of small-size mitochondria. These cells can be easily acquired from the peripheral blood with minimal invasion via simple venipuncture. The present study aimed to investigate whether transplantation of platelet-derived mitochondria (Mito-Plt) could improve DACI. Cognitive behaviors were assessed using the Morris water maze test in db/db mice. The results demonstrated that Mito-Plt was internalized into hippocampal neurons 24 h following intracerebroventricular injection. Importantly, one month following Mito-Plt transplantation, DACI was alleviated in db/db mice and the effect was accompanied with increased mitochondrial number, restored mitochondrial function, attenuated oxidative stress and neuronal apoptosis, as well as decreased accumulation of Aβ and Tau in the hippocampus. Taken together, the data demonstrated that transplantation of Mito-Plt attenuated cognitive impairment and mitochondrial dysfunction in db/db mice. This method may be a potential therapeutic application for the treatment of DACI.

Porous metal-organic frameworks (MOFs) have demonstrated a great potential in proton conduction and luminescence sensing due to functionalized nodes, ligands and channels, or pores. Herein, we prepared a hydrothermally stable Eu-MOF that also resisted acid and base using a bifunctional organic ligand containing carboxylic acid groups, which are easily coordinated to Eu ions, and Eu-phobic tetrazolyl groups as potential proton-hopping sites. The hydrogen bond network, which was constructed by the uncoordinated anionic tetrazolium and the coordinated and free water molecules, endowed this Eu-MOF with the highest proton conductivity of 4.45 × 10-2 S/cm at 373 K and 93% relative humidity. The proton conductivity of the Nafion membrane containing this Eu-MOF increased 1.74 times. More interestingly, the hybrid membrane displayed luminescence pH sensing because the changeable protonation levels of uncoordinated tetrazolium groups along with the pH tuned the emission of embedded Eu-MOFs. Such a dual-functional MOF-based hybrid membrane including proton conduction and pH sensing is reported for the first time, which could open an avenue to the more practical application for functional MOFs.

Background Gestational diabetes mellitus (GDM) is the most common metabolic disorder that can occur during pregnancy and is associated with a long-term risk of both maternal and neonatal comorbidities. This study aimed to investigate the association between echocardiographic epicardial adipose tissue (EAT) and the risk for GDM during the early second trimester of pregnancy. Method We recruited all singleton pregnancies between January 2014 and December 2018 at 16 weeks + 0 days to 19 weeks + 6 days. We then used generalized linear models to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for EAT as a potential predictor for GDM. Receiver-operating-characteristic (ROC) analysis was then conducted to investigate the discriminative capacity of any individual maternal factor for the prediction of GDM. Results In total, our study involved 314 pregnant women with GDM and 1832 pregnant women without GDM. Multivariate regression analysis revealed that EAT thickness (OR = 2.87; 95% CI: 2.49–3.31) was significantly associated with the presence of GDM (P < 0.001). Furthermore, EAT thickness was also significantly associated with a range of adverse outcomes in the GDM group, including large size for gestational age, neonatal hypoglycemia, admission to the neonatal intensive care unit, preterm delivery, and hyperbilirubinemia (P < 0.001). ROC analysis revealed that the area under the curve was 0.790 (95% CI: 0.768–0.812). When the cutoff value for EAT thickness was set to 5.49 mm, the sensitivity was 95.2% and the specificity was 50.5%. Conclusions Echocardiographic EAT thickness is positively and significantly associated with both the risk of GDM and adverse outcomes related to GDM. Echocardiographic EAT has the potential to predict GDM prior to actual clinical diagnosis.

Many large-flow fires need sustained and stable water supplies, which are often challenging because of the complex fire ground. To analyze the causes and influencing factors of pressure fluctuation in the process of switching water sources, an experimental setup and a simulation model were established. The basic hydraulics principle is that pressure drop occurs during the switching between two channels. The pressure drop magnitude in the main pipe was studied for various resistance values of the main pipe, switching time of the control valve in the channel, the number of parallel channels, and the diameter of the clustered pipe. The results show that three key parameters affect the magnitude of the pressure drop: a longer switching time, less pipe resistance, and more parallel channels can minimize the pressure drop magnitude of the mine pipe. This study provides fire equipment designer with information about the influence of different variables on the pressure drop magnitude of the main pipe and basic design criteria to minimize these effects. Meanwhile, a simple scheme is proposed to minimize the large pressure fluctuation, and the pressure fluctuation can be decreased and even eliminated to some extent.

An infrared image-based feedback control system for intelligent fire monitor was proposed in this paper, whose aims are to realize automatic aiming of the fire site and continuous fire tracking in the process of fire extinguishing through adjusting of the yaw angle of the fire monitor. Firstly, infrared camera was used to capture images of the fire site, and under high temperatures, the fire would be easily observed. An improved adaptive image threshold segmentation scheme was developed for image background segmentation and fire acquisition. The fire region in the captured image was considered as region of interest (ROI) and the geometric center of which was calculated. Due to the special advantageous structural design of the proposed system, the geometric center of ROI representing the actual fire was considered as the direction which the fire monitor should aim at. Furthermore, a fuzzy control scheme simulating the operating mode of firefighters was proposed, and the ROI center position deviation and deviation rate were considered as inputs of the control system. Experimental results show that, after the fire was detected by the system, the fire monitor could be yawed to the direction of the fire within 2000 ms, and the deviation of image position is less than 1.6 pixels. Similarly, the proposed system could be well adapted to the changing position of the fire caused by combustion. And after no more than image iteration of three frames, the controller realizes aiming of the fire by the fire monitor, and the final deviation is less than 0.1 pixels. In general, the proposed infrared image feedback control system for fire horizontal position aiming of intelligent fire monitor has great potential for intelligent fire extinguishing by fire-fighting robots.

The radiation exposure received by astronauts on deep-space missions must be considered in the design of future spacecraft. Galactic cosmic rays (GCR) include high-energy heavy ions, which can often penetrate the thickest shielding that can be practically launched with a spacecraft. Some of these heavy ions deliver a very high radiation dose per particle, and this highly ionizing radiation is more damaging per unit dose than sparsely ionizing radiation. Nuclear fragmentation is the principal physical mechanism by which the dose delivered by these particles can be reduced. This study reports models of the interactions of heavy ions from galactic cosmic rays in a manned spacecraft. The models were built and analyzed in the Geant4 toolkit. The total and secondary particle dose depth distributions in a water phantom were estimated using Monte-Carlo simulation. Heavy ions of 400 A MeV 12C, 600 A MeV 16O, 1000 A MeV 28Si, 1000 A MeV 56Fe were modeled as traversing a simple spacecraft wall to reach a water phantom. The simulations predict the Bragg curves of different forms of radiation in the water phantom. This curve is used to calculate the contributions to the total radiation dose from charged secondary fragments, primary-beam nuclei, and other particles produced from nuclear fragmentation reactions.

Grazing is an important driving factor for soil carbon sequestration in grasslands. The objective of this study was to clarify the effects of grazing and its intensity on soil organic carbon stocks (SOCS) in the revegetated grasslands in the Loess Plateau region. With the grasslands excluded from grazing ＞20 years as the control, we investigated the SOCS in the 0-20 cm soil layer in three grazing intensities in the west, middle, and east of wind-water erosion crisscross region and the water erosion region. The intensity of grazing in the study was represented by the number of goat dung in the sampling sites, which included 0-10, 10-20, and more than 20 goat dung·m放牧是影响草地土壤碳固存的重要因素。本研究选取黄土高原水蚀风蚀交错区西部、中部、东部地区及水蚀区,以各区20年以上退耕封禁地为对照,分析3个放牧强度下(羊粪球密度分别为0～10、10～20、20 ind·m

Phellinus spp., an important medicinal fungus mushroom extensively cultivated and consumed in East Asia for over 2000 years, is traditionally considered a precious food supplement and medicinal ingredient. Published studies showed that the polysaccharides are major bioactive macromolecules from Phellinus spp. (PPs) with multiple health-promoting effects, including immunomodulatory, anti-cancer, anti-inflammatory, hepatoprotective, hypoglycemic, hypolipidemic, antioxidant, and other bioactivities. Although the polysaccharides extracted from the fruiting body, mycelium, and fermentation broth of Phellinus spp. have been extensively studied for the extraction and purification methods, structural characteristics, and pharmacological activities, the knowledge for their structures and bioactivity relationship, toxicologic effects, and pharmacokinetic profile is limited. This review systematically summarizes the recent progress in the isolation and purification, chemical structures, bioactivities, and the underlying mechanisms of PPs. Information from this review provides insights into the further development of polysaccharides from PPs as therapeutic agents and functional foods.

Previous studies have detected the correlation of polymorphisms in regulatory T cells associated genes

Plant-based dairy alternatives are gaining increasing interest, e.g. alternatives to yoghurt, cheese, and butter. In all these products butter flavor (diacetyl + acetoin) plays an important role. We previously have reported efficient butter flavor formation from low value dairy side streams using a dairy isolate of Lactococcus lactis deficient in lactate dehydrogenase. Here, we have tested the ability of this strain, RD1M5, to form butter flavor in plant milks based on oat and soy. We found that oat milk, with its high sugar content, supported more efficient production of butter aroma, when compared to soy milk. When supplemented with glucose, efficient butter aroma production was achieved in soy milk as well. We also carried out an extended adaptive laboratory evolution of the dairy strain in oat milk. After two months of adaptation, we obtained a strain with enhanced capacity for producing butter aroma. Despite of its high sugar content, RD1M5 and its adapted version only metabolized approximately 10% of the fermentable sugars available in the oat milk, which we found was due to amino acid starvation and partly starvation for vitamins. The study demonstrates that dairy cultures have great potential for use in plant-based fermentations.

Previous studies have detected the correlation of polymorphisms in regulatory T cells associated genes FOXP3 and CTLA-4 with pre-eclampsia (PE) risk, but the results are inconsistent among studies. Eligible studies were retrieved in several database. Odds ratios (ORs) with 95% confidence intervals (CIs) were utilised to evaluate the relationship between FOXP3 rs3761548, FOXP3 rs2232365, CTLA-4 rs231775 polymorphisms, and PE susceptibility in the genetic models. The subgroup analysis and trial sequential analysis were performed. Twelve studies with a total of 4658 participants were included. There was a statistically significant association between FOXP3 rs3761548 polymorphism and PE within the recessive model in Asian (OR = 0.54, 95% CI = 0.34–0.86). Trial sequential analysis indicated sufficient proof of such association in the Asian population. This meta-analysis provides sufficient statistical evidence indicating an association between FOXP3 rs3761548 polymorphism and PE risk in Asian.IMPACT STATEMENTWhat is already known on this subject?FOXP3 and CTLA4 are markers of regulatory T cells which play a crucial role during a preeclamptic pregnancy.What do the results of this study add? Eleven studies with a total of 4658 participants were included. There was a statistically significant association between FOXP3 rs3761548 polymorphism and pre-eclampsia (PE) within the recessive model in Asian (OR = 0.54, 95% CI = 0.34–0.86). Trial sequential analysis indicated sufficient proof of such association in the Asian population. However, there was no enough evidence could proof significant association between FOXP3 rs2232365 or CTLA-4 rs231775 polymorphism and PE.What are the implications of these findings for clinical practice and/or further research? This meta-analysis provides sufficient statistical evidence indicating an association between FOXP3 rs3761548 polymorphism and PE risk in Asian. The findings in this study may provide a basis for the further study on FOXP3 rs3761548 polymorphism in future research. What is already known on this subject?FOXP3 and CTLA4 are markers of regulatory T cells which play a crucial role during a preeclamptic pregnancy. What do the results of this study add? Eleven studies with a total of 4658 participants were included. There was a statistically significant association between FOXP3 rs3761548 polymorphism and pre-eclampsia (PE) within the recessive model in Asian (OR = 0.54, 95% CI = 0.34–0.86). Trial sequential analysis indicated sufficient proof of such association in the Asian population. However, there was no enough evidence could proof significant association between FOXP3 rs2232365 or CTLA-4 rs231775 polymorphism and PE. What are the implications of these findings for clinical practice and/or further research? This meta-analysis provides sufficient statistical evidence indicating an association between FOXP3 rs3761548 polymorphism and PE risk in Asian. The findings in this study may provide a basis for the further study on FOXP3 rs3761548 polymorphism in future research.

Hydrological model is an effective tool for hydrological research. The initial abstraction ratio (水文模型是水文过程研究的有效工具,初损率(

Bacterial smear-ripened cheeses are commonly contaminated with fungi and the potentially dangerous pathogen Listeria monocytogenes, which compromises food safety and quality. Here, we present new approach for accelerating smear development on these cheeses, while reducing growth of unwanted microorganisms. We grew the smear microorganism Brevibacterium linens in acid whey (AW) supplemented with a by-product of whey protein hydrolysate production (FCH). B. linens grew to a high cell density (10.27 log 10 CFU/mL), and alkalized the AW-FCH medium. We found that the fermented AW-FCH, with its high pH, rapidly killed L. monocytogenes and yeasts. When culture medium was smeared on the cheese surface, the surface pH was rapidly neutralized, and in just 7 days, the cheeses developed a dense orange color and growth of spoilage fungi was inhibited. Summing up, by growing B. linens to high cell density on low-value dairy byproducts, and applying dense suspensions onto cheeses, we can reduce the time needed for establishing the smear. Added benefits are increased food safety and less spoilage, as quick smear development inhibits growth of unwanted microorganisms on the rind. Preliminary tests at a dairy show that flavor development is comparable to traditional bacterial surface-ripened cheeses with far more complex surface microflora.