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Bacterial lipoproteins are a set of membrane proteins with various functions; many of which are virulence factors of pathogenic bacteria. In the present study, we investigated the role of an outer membrane lipoprotein Lip40 in the pathogenesis of Actinobacillus pleuropneumoniae. A mutant strain (Δlip40) lacking Lip40 and a complemented strain (CΔlip40) were constructed. Δlip40 exhibited reduced adherence to the St. Jude porcine lung cells. The ability of the Δlip40 mutant to colonize the mouse lung tissues was significantly impaired compared to that of the wild type and complementation strains. Furthermore, an infection assay revealed that pigs infected with Δlip40 showed fewer clinical signs and lung lesions, indicating that Lip40 contributed to the development of porcine pleuropneumonia. Collectively, our data suggest that Lip40 is involved in the virulence of A. pleuropneumoniae. [ABSTRACT FROM AUTHOR]

Clematis apiifolia, belonging to the Clematis L., is a woody vine native to China. It is characterized as heat resistant and fast growing. To better understand potential mechanisms involved in heat-stress responses in Clematis, we characterized the digital gene expression signatures of C. apiifolia under heat-stress conditions. Using RNA sequencing technology, we sequenced six libraries, three biological replicates of control samples and three of heat-stressed samples. In total, 61,708 unigenes were obtained, 36,447 (59.06%) of which were annotated. There were 1941 differentially expressed genes (DEGs) under heat stress, including 867 upregulated and 1074 downregulated genes. Gene ontology enrichment of DEGs revealed that 'metabolic process,' 'cellular process,' and 'single organism' were the top three functional terms under heat stress. A Kyoto Encyclopedia of Genes and Genomes analysis led to the identification of 'protein processing in metabolic pathways,' 'phenylpropanoid biosynthesis,' and 'biosynthesis of secondary metabolites' as significantly enriched pathways. Among the upregulated genes, heat-shock factors and heat-shock proteins, especially small heat-shock proteins, were particularly abundant under heat stress. The data will aid in elucidating the molecular events underlying heat-stress responses in Clematis L. [ABSTRACT FROM AUTHOR]

Crape myrtle ( Lagerstroemia indica) is a woody ornamental plant popularly grown because of its long-lasting, midsummer blooms and beautiful colors. The GL1 dominant mutant is the first chlorophyll-less mutant identified in crape myrtle. It was obtained from a natural yellow leaf bud mutation. We previously revealed that leaf color of the GL1 mutant is affected by light intensity. However, the mechanism of the GL1 mutant on light response remained unclear. The acclimation response of mutant and wild-type (WT) plants was assessed in a time series after transferring from low light (LL) to high light (HL) by analyzing chlorophyll synthesis precursor content, photosynthetic performance, and gene expression. In LL conditions, coproporphyrinogen III (Coprogen III) content had the greatest amount of accumulation in the mutant compared with WT, increasing by 100%. This suggested that the yellow leaf phenotype of the GL1 dominant mutant might be caused by disruption of coproporphyrinogen III oxidase (CPO) biosynthesis. Furthermore, the candidate gene, oxygen-independent CPO ( HEMN), might only affect expression of upstream genes involved in chlorophyll metabolism in the mutant. Moreover, two genes, photosystem II (PSII) 10 kDa protein ( psbR) and chlorophyll a/b binding protein gene ( CAB1), had decreased mRNA levels in the GL1 mutant within the first 96 h following LL/HL transfer compared with the WT. Hierarchical clustering revealed that these two genes shared a similar expression trend as the oxygen-dependent CPO ( HEMF). These findings provide evidence that GL1 is highly coordinated with PSII stability and chloroplast biogenesis. [ABSTRACT FROM AUTHOR]

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Crop residues are effective for the prevention of soil erosion. The crop residue cover (CRC) can be mapped by remote sensing. Different morphologies of crop residue will affect the spectral reflectance, reducing the accuracy of CRC estimation by multispectral data. However, the influence of residue morphology is not fully considered on the accuracy of CRC mapping using satellite images. In addition, the spectral indices are easily saturated and less sensitive to high-density areas of crop residue. This study selected four maize planting sites to obtain hyperspectral reflectance and unmanned aerial vehicle (UAV) images. The effects of CRC and residue morphology on spectral reflectance were analyzed, and a new Residue Adjust Normalized Difference Residue Index (RANDRI) was proposed. UAV images were used to extract ground CRC data for training a CRC prediction model based on Sentinel-2 MSI data. Finally, the piecewise prediction model based on different residue indices was used to map CRC. The study results highlighted a linear relationship between the reflectance intersection of shortwave infrared 2 reflectance and red edge 3 of Sentinel-2 MSI with different residual morphologies, called the residue line. The model accuracy of RANDRI optimized by residual line parameters was better than that of the Normalized Difference Residue Index and Soil Adjust Normalized Difference Residue Index (SANDRI) in the high-density area of crop residue. RANDRI can weaken the influence of residue morphologies on modeling accuracy. The CRC spatial distribution by the piecewise SANDRI+RANDRI model was more consistent with CRC measured than that of the RANDRI models individually. The determination coefficient of the piecewise model was 0.82, and the relative error was 10.66%. The piecewise model can effectively improve the anti-saturation ability of the spectral indices. We suggest a rapid and accurate approach for monitoring the CRC and provide a more suitable CRC mapping strategy for high-density areas of crop residue using multispectral remote sensing data. • Crop residue cover was mapped using remote sensing. • The effects of crop residue cover and residue morphology on spectral reflectance were analyzed. • Novel crop residue cover prediction models were suggested and analyzed. [ABSTRACT FROM AUTHOR]

The trade-off/synergy between planned ecological land and farmland is of great significance to food security, economic development and ecological environment protection in black soil region, and has become a global strategic issue related to the sustainable development of human society. Land use/cover (LUC) simulation model can be used to formulate sustainable land use strategies that balance farmland use and ecological environment protection. In this study, we combined the grey attribute of land resources with the complexity of land use/cover change (LUCC). By integrating grey Markov model and Future Land Use Simulation (FLUS) model, we discussed the characteristics of historical LUCC in Songnen Plain from 2000 to 2020, and simulated the LUC pattern of Songnen Plain in 2040 under different scenarios. At the same time, the landscape gradient evolution of ecological land and farmland under different scenarios from 2000 to 2020 and 2040 were quantitative analyzed in order to clarify the changes of ecological land and farmland in the study area in the past and future, which provided a basis for the tradeoff / synergy between farmland and ecological land. The main conclusions are as follows: (1) The LUCC in 2020 was simulated with the Kappa coefficient was 0.82, the figure of merit (FOM) coefficient was 0.31, which verified the rationality of the accuracy of the model. (2) Whether it is baseline development (BL) scenario, farmland protection (FP) scenario, ecological protection (EP) scenario and black land protection (BP)scenario, the transformation of farmland and ecological land was distributed in the central and western areas, the transformation frequency of farmland, wetland and grassland was higher. (3) Landscape gradient analysis showed the degree of fragmentation of ecological land in Songnen Plain was high, and the anti-interference ability of farmland is strong. (4) The whole region is a trade-off between the two landscape, and the trade-off in the typical black soil area is about 20% higher than the non-typical black soil area. Black land protection scenario may be the best solution to coordinate the sustainable development of farmland and ecological land in the future. These studies will not only provide a theoretical basis for regional land management decision-making and urban planning and construction, but also provide some reference for its economic construction and sustainable development. [ABSTRACT FROM AUTHOR]

In order to recover and utilize the potential energy of mining trucks efficiently, this paper proposes a nested optimization method of a novel energy storage system. By analyzing the multi-objective optimization problem of the oil-circulating hydro-pneumatic energy storage system, a nested optimization method based on the advanced adaptive Metamodel-based global optimization algorithm is carried out. Research shows that this method only requires a short time to solve the complex nonlinear hybrid optimization problem and achieves better results. The optimized energy storage system has higher system efficiency, energy density, and volume utilization rate, thus obtaining a smaller system volume and weight. Verified by the bench experiment of its powertrain, the hydro-pneumatic hybrid mining truck with the optimized energy storage system significantly reduces its fuel consumption and CO2 emission. Thus, it lays the foundation for the practical application of hydro-pneumatic hybrid mining trucks. [ABSTRACT FROM AUTHOR]

Key message: The study established split-root system (SRS) in foxtail millet, and identified the molecular regulatory mechanisms and metabolic pathways related to systemic nitrogen signaling based on this system and transcriptome analysis. The growth of crops is primarily constrained by the availability of nitrogen (N), an essential nutrient. Foxtail millet (Setaria italica L.) is a significant orphan crop known for its strong tolerance to barren conditions. Despite this, the signaling pathway of nitrogen in foxtail millet remains largely unexplored. Identifying the candidate genes responsible for nitrogen response in foxtail millet is crucial for enhancing its agricultural productivity. This study utilized the split-root system (SRS) in foxtail millet to uncover genes associated with Systemic Nitrogen Signaling (SNS). Transcriptome analysis of the SRS revealed 2158 differentially expressed genes (DEGs) implicated in SNS, including those involved in cytokinin synthesis, transcription factors, E3 ubiquitin ligase, and ROS metabolism. Silencing of SiIPT5 and SiATL31 genes through RNAi in transgenic plants resulted in reduced SNS response, indicating their role in the nitrogen signaling pathway of foxtail millet. Furthermore, the induction of ROS metabolism-related genes in response to KNO3 of the split-root System (Sp.KNO3) suggests a potential involvement of ROS signaling in the SNS of foxtail millet. Overall, this study sheds light on the molecular regulatory mechanisms and metabolic pathways of foxtail millet in relation to SNS. [ABSTRACT FROM AUTHOR]

In addition to preventing precocious larval metamorphosis, juvenile hormone (JH), synthesized in corpora allata (CA), is known to stimulate female reproduction of insects. JH titer is extremely low or absent during metamorphosis, but thereafter rapidly increases in the previtellogenic stage and rises to a peak in the vitellogenic phase. However, the mechanisms underlying the biosynthesis of high levels of JH in adults remain unclear. We found in this study that 12 genes involved in JH synthesis pathway were highly expressed in the CA of adult locusts. By transcriptome analysis and quantitative real‐time – polymerase chain reaction validation, a total of 106 evolutionary conserved micro RNAs (miRNAs) and 163 species‐specific miRNAs were identified in locust CA. Dual‐luciferase assay revealed that 17 miRNAs bound to 10 JH synthesis genes (JHSGs) and downregulated their expression. These miRNAs were expressed in low levels during vitellogenic stage, which was oppositive from that of targeting JHSGs. Six miRNAs including miR‐971‐3p, miR‐31a, miR‐9‐5p, miR‐1‐3p, miR‐315, and miR‐282 were selected for function study. Co‐application of agomiRs resulted in significantly decreased levels of targeting JHSGs, accompanied by significantly reduced vitellogenin expression as well as arrested ovarian development. The data suggest that multiple miRNAs expressed synchronously at low levels in the vitellogenic phase, thereby ensuring the high levels of JHSG expression to facilitate JH biosynthesis required for JH‐dependent female reproduction. The findings provide important information for deciphering miRNA—messenger RNA modules for JH biosynthesis as well as JH regulation of insect metamorphosis and reproduction. [ABSTRACT FROM AUTHOR]

• This article discusses the monitoring of physiological indicators during exercise. • The method has good computational performance and dynamic load balancing. • The model can be used to reveal the changes associated with SIRS. This article discusses the monitoring of physiological indicators during exercise, combined with the data fusion algorithm of the smart city Internet of Things health. We use the hash value of the tuple key to the corresponding data block of the node, use the data block record to obtain the response of the target node, and output the data tuple. It is used as a measure of the load balance of health data streams to determine whether load migration is needed and to determine the way and amount of migration tasks to make migration decisions. The simulation experiments show that the method has good computational performance and dynamic load balancing. A series of mean arterial pressure and heart rate of patients and non-stationary health data, and a series of blood pressure and heart rate of health individuals in different postures are selected to perform experiments to analyze the transfer function and power spectra in the model, validating that the model can be used to reveal the changes associated with severe systemic response syndrome (SIRS), providing a hypothesis for the decomposition of autoregulation of physiological control under health and disease conditions. [ABSTRACT FROM AUTHOR]

Amino acid permeases (AAPs) transporters are crucial for the long-distance transport of amino acids in plants, from source to sink. While Arabidopsis and rice have been extensively studied, research on foxtail millet is limited. This study identified two transcripts of SiAAP9, both of which were induced by NO3− and showed similar expression patterns. The overexpression of SiAAP9L and SiAAP9S in Arabidopsis inhibited plant growth and seed size, although SiAAP9 was found to transport more amino acids into seeds. Furthermore, SiAAP9-OX transgenic Arabidopsis showed increased tolerance to high concentrations of glutamate (Glu) and histidine (His). The high overexpression level of SiAAP9 suggested its protein was not only located on the plasma membrane but potentially on other organelles, as well. Interestingly, sequence deletion reduced SiAAP9's sensitivity to Brefeldin A (BFA), and SiAAP9 had ectopic localization on the endoplasmic reticulum (ER). Protoplast amino acid uptake experiments indicated that SiAAP9 enhanced Glu transport into foxtail millet cells. Overall, the two transcripts of SiAAP9 have similar functions, but SiAAP9L shows a higher colocalization with BFA compartments compared to SiAAP9S. Our research identifies a potential candidate gene for enhancing the nutritional quality of foxtail millet through breeding. [ABSTRACT FROM AUTHOR]

Simple Summary: Global climate change is a growing threat to the lives and ranges of reptile species. The present study examines three lizard species (Calotes versicolor, Ateuchosaurus chinensis, and Hemidactylus bowringii) that share a sympatric distribution in the Guangzhou region of Guangdong, China. Mitochondria act as energy-producing centers, and the present study analyses the effects of low temperatures in the context of warm winters on lizards, by examining their responses to low-temperature stress at the level of mitochondrial gene expression. This present study analyses the effects of low temperatures in the context of warm winters on the mitochondrial gene expression of lizards and reveals the implications of the distribution within it. Ecological factors related to climate extremes have a significant influence on the adaptability of organisms, especially for ectotherms such as reptiles that are sensitive to temperature change. Climate extremes can seriously affect the survival and internal physiology of lizards, sometimes even resulting in the loss of local populations or even complete extinction. Indeed, studies have shown that the expression levels of the nuclear genes and mitochondrial genomes of reptiles change under low-temperature stress. At present, the temperature adaptability of reptiles has rarely been studied at the mitochondrial genome level. In the present study, the mitochondrial genomes of three species of lizards, Calotes versicolor, Ateuchosaurus chinensis, and Hemidactylus bowringii, which live in regions of sympatry, were sequenced. We used RT-qPCR to explore the level of mitochondrial gene expression under low-temperature stress, as compared to a control temperature. Among the 13 protein-coding genes (PCGs), the steady-state transcript levels of ND4L, ND1, ATP6, and COII were reduced to levels of 0.61 ± 0.06, 0.50 ± 0.08, 0.44 ± 0.16, and 0.41 ± 0.09 in C. versicolor, respectively, compared with controls. The transcript levels of the ND3 and ND6 genes fell to levels of just 0.72 ± 0.05 and 0.67 ± 0.05 in H. bowringii, compared with controls. However, the transcript levels of ND3, ND5, ND6, ATP6, ATP8, Cytb, and COIII genes increased to 1.97 ± 0.15, 2.94 ± 0.43, 1.66 ± 0.07, 1.59 ± 0.17, 1.46 ± 0.04, 1.70 ± 0.16, and 1.83 ± 0.07 in A. chinensis. Therefore, the differences in mitochondrial gene expression may be internally related to the adaptative strategy of the three species under low-temperature stress, indicating that low-temperature environments have a greater impact on A. chinensis, with a small distribution area. In extreme environments, the regulatory trend of mitochondrial gene expression in reptiles is associated with their ability to adapt to extreme climates, which means differential mitochondrial genome expression can be used to explore the response of different lizards in the same region to low temperatures. Our experiment aims to provide one new research method to evaluate the potential extinction of reptile species in warm winter climates. [ABSTRACT FROM AUTHOR]

Brainbow is a genetic cell-labeling technique that allows random colorization of multiple cells and real-time visualization of cell fate within a tissue, providing valuable insights into understanding complex biological processes. However, fluorescent proteins (FPs) in Brainbow have distinct excitation spectra with peak difference greater than 35 nm, which requires sequential imaging under multiple excitations and thus leads to long acquisition times. In addition, they are not easily used together with other fluorophores due to severe spectral bleed-through. Here, we report the development of a single-wavelength excitable Brainbow, UFObow, incorporating three newly developed blue-excitable FPs. We have demonstrated that UFObow enables not only tracking the growth dynamics of tumor cells in vivo but also mapping spatial distribution of immune cells within a sub-cubic centimeter tissue, revealing cell heterogeneity. This provides a powerful means to explore complex biology in a simultaneous imaging manner at a single-cell resolution in organs or in vivo. UFObow is a single-wavelength excitable Brainbow technique, incorporating three newly developed blue-excitable fluorescent proteins. This method facilitates mapping of immune cells' spatial distribution at a single-cell resolution. [ABSTRACT FROM AUTHOR]

Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution, and is commonly employed in wheat breeding programs. Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination, but the underlying mechanism is poorly understood. Here, we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid (Triticum durum, AABB) and hexaploid wheat (Triticum aestivum, AABBDD). In the paternal‐ versus the maternal‐excess cross, the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed, causing reduced germination percentage. The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types. Furthermore, expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses, leading to increased number of imprinted genes. The endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA methylation. Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019, leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross, as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats (CRISPR) – CRISPR‐associated protein 9 generated hexaploid mutants. These findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs. [ABSTRACT FROM AUTHOR]