Beef intramuscular fatty acid (FA) profile was investigated on a sample of Simmental beef bulls from an intensive rearing system in Croatia. Animals were housed indoor in common pens on a concrete slatted floor (SF group, n=15) or a full floor with straw bedding (FF group, n=15). Diet in both groups was given as total mixture ration composed from maize grain and stalk silage, super-concentrate and hay (average composition per kg: 599 g of DM, 76 g of crude protein and 4.59 MJ of ME as feed). Bulls were slaughter at similar age (494±17 days) and body weight (597.5±56.4 kg) and the intramuscular fat (IMF) content (g/kg) and FA composition (g/100g of total FA) of the M. longissimus thoracis were determined. The average IMF content was 25.49±9.21 with C18:1 (36.28±3.99), C16:0 (22.61±1.81), C18:0 (17.18±1.71) and C18:2n-6 (9.15±3.08) as the most represented FAs. The average proportions of total saturated FA (SFA) and monounsaturated FA (MUFA) were 44.33±1.89 and 41.78±4.31, while the share of total polyunsaturated FA (PUFA) was 13.89±4.72, with the averages of n-6 and n-3 PUFA totals of 12.93±4.52 and 0.68±0.24, respectively. The PUFA/SFA and n-6/n-3 ratios were out of the nutritionally recommended limits; while the proportion of beneficial conjugated linoleic acid (CLA) averaged 0.285±0.082 g/100g of FA. Floor type had no major effect on FA profile, except for slightly higher CLA and C20:1 and lower C12 and n-6/n-3 in SF group. It is concluded that the obtained results imply a need for feeding strategy that could enhance the nutritional quality of beef FA under the intensive production systems., Profil intramuskularnih masnih kiselina (MK) mesa goveda istraživan je na uzorku simentalskih tovnih bikova iz intenzivnog sustava uzgoja u Hrvatskoj. Smještaj životinje bio je unutar objekata u zajedničkim betonskim boksovim na rešetkastom podu (RP grupa, n=15) ili na punom podu uz steljenje (PP grupa, n=15). Hranidba u obje grupe bila je u obliku potpuno izmiješanog obroka sastavljenog iz silaže kukuruznog zrna i stabljike, super koncentrata i sijena (prosječni sastav kg obroka: 599 g ST, 76 g sirovog proteina i 4,59 MJ ME). Bikovi su zaklani pri sličnoj dobi (494±17 dana) i tjelesnoj masi (597,5±56,4 kg) te je određen sadržaj intramuskularne masti (IMM, g/kg) i sastav MK (g/100g of total FA) M. longissimus thoracis-a. Prosječan sadržaj IMM iznosio je 25,49±9,21, dok su najzastupljenije MK bile C18:1 (36,28±3,99), C16:0 (22,61±1,81), C18:0 (17,18±1,71) i C18:2n-6 (9,15±3,08). Prosječni sadržaji ukupnih zasićenih MK (ZMK) i mono-nezasićenih MK (MNK) iznosili su 44,33±1,89 i 41,78±4,31, dok je sadržaj ukupnih poli-nezasićenih MK (PNMK) bio 13,89±4,72, s prosjecima ukupnih n-6 i n-3 PNMK od 12,93±4,52, odnosno 0,68±0,24. Omjeri PNMK/ZMK i n-6/n-3 bili su izvan nutritivno preporučenih vrijednosti, dok je sadržaj korisne konjugirane linolne kiseline (KLK) u prosjeku iznosio 0,285±0,082 g/100g ukupnih MK. Tip poda nije imao značajniji utjecaj na MK profil, osim nešto višeg sadržaja KLK i C20:1 te nižeg sadržaja C12:0 i n-6/n-3 omjera u RP grupi. Zaključeno je da dobiveni rezultati ukazuju na potrebu za hranidbenom strategijom koja može poboljšati nutritivnu kakvoću MK u goveđem mesu iz intenzivnog sustava proizvodnje.