3,062 results on '"Fazakerley, A."'
Search Results
152. Observations of a Unique Cusp Signature at Low and Mid Altitudes
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Keith, W. R., Winningham, J. D., Goldstein, M. L., Wilber, M., Fazakerley, A. N., Rème, H., Fritz, T. A., Balogh, A., Cornilleau-Wehrlin, N., Maksimovic, M., Fritz, Theodore A., editor, and Fung, Shing F., editor
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- 2005
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153. Semliki Forest Virus Induced Demyelination
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Fazakerley, John K., Lavi, Ehud, editor, and Constantinescu, Cris S., editor
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- 2005
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154. Cluster observations of the substructure of a flux transfer event: analysis of high-time-resolution particle data
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A. Varsani, C. J. Owen, A. N. Fazakerley, C. Forsyth, A. P. Walsh, M. André, I. Dandouras, and C. M. Carr
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Flux transfer events (FTEs) are signatures of transient reconnection at the dayside magnetopause, transporting flux from the dayside of the magnetosphere into the magnetotail lobes. They have previously been observed to contain a combination of magnetosheath and magnetospheric plasma. On 12 February 2007, the four Cluster spacecraft were widely separated across the magnetopause and observed a crater-like FTE as they crossed the Earth's dayside magnetopause through its low-latitude boundary layer. The particle instruments on the Cluster spacecraft were in burst mode and returning data providing 3-D velocity distribution functions (VDFs) at 4 s resolution during the observation of this FTE. Moreover, the magnetic field observed during the event remained closely aligned with the spacecraft spin axis and thus we have been able to use these 3-D data to reconstruct nearly full pitch angle distributions of electrons and ions at high time resolution (up to 32 times faster than available from the normal mode data stream). These observations within the boundary layer and inside the core of the FTE show that both the interior and the surrounding structure of the FTE consist of multiple individual layers of plasma, in greater number than previously identified. Our observations show a cold plasma inside the core, a thin layer of antiparallel-moving electrons at the edge of FTE itself, and field-aligned ions with Alfvénic speeds at the trailing edge of the FTE. We discuss the plasma characteristics in these FTE layers, their possible relevance to the magnetopause reconnection processes and attempt to distinguish which of the various different FTE models may be relevant in this case. These data are particularly relevant given the impending launch of NASA's MMS mission, for which similar observations are expected to be more routine.
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- 2014
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155. In-flight calibration of the Cluster PEACE sensors
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N. Doss, A. N. Fazakerley, B. Mihaljčić, A. D. Lahiff, R. J. Wilson, D. Kataria, I. Rozum, G. Watson, and Y. Bogdanova
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Geophysics. Cosmic physics ,QC801-809 - Abstract
The Plasma Electron and Current Experiment (PEACE) instruments operate on all four of the Cluster spacecraft and measure the 3-D velocity distribution of electrons in the energy range from 0.59 eV to 26.4 keV during each spacecraft spin. Pitch angle distributions and moments of the velocity distribution are also produced. As the mission has progressed, the efficiency of the detectors has declined. Several factors may play a role in this decline such as exposure to radiation, high electron fluxes and spacecraft thruster firings. To account for these variations, continuous in-flight calibration work is essential. The purpose of this paper is to describe the PEACE calibration parameters, focussing in particular on those that vary over time, and to describe the methods which are used to determine their evolution.
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- 2014
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156. Evidence of transient reconnection in the outflow jet of primary reconnection site
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R. Wang, R. Nakamura, T. Zhang, A. Du, W. Baumjohann, Q. Lu, and A. N. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The precise mechanism for the formation of magnetic islands in the magnetotail and the subsequent evolution are still controversial. New investigations have provided the first observational evidence of secondary reconnection in the earthward outflow jet of primary reconnection in the magnetotail. The secondary reconnection takes place 38 c/ωpi earthward from the primary reconnection site and results in the birth of a magnetic island observed. This generation mechanism is different from the widely used model of multiple reconnection X-lines. The duration of the secondary reconnection was approximate one ion gyration period (5 s). The observations resemble recent numerical simulations where magnetic reconnection could spontaneously and transiently happen in the outflow jet, called secondary reconnection, which was used to explain the formation of the dipolarization fronts. Coincidentally, another magnetic island moving earthward passed through three satellites successively. By this chance we find the magnetic island was accelerated towards Earth with an acceleration of about 9 km s−2 at −19 RE in the magnetotail.
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- 2014
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157. Multi-Point Cluster Observations of VLF Risers, Fallers and Hooks at and Near the Plasmapause
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Pickett, J. S., Santolík, O., Kahler, S. W., Masson, A., Adrian, M. L., Gurnett, D. A., Bell, T. F., Laakso, H., Parrot, M., Décréau, P., Fazakerley, A., Cornilleau-Wehrlin, N., Balogh, A., André, M., Sauvaud, Jean-André, editor, and Němeček, Zdeněk, editor
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- 2004
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158. Semliki Forest virus infection of laboratory mice: a model to study the pathogenesis of viral encephalitis
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Fazakerley, John K., Calisher, Charles H., editor, and Griffin, Diane E., editor
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- 2004
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159. Emerging trends in informal sector recycling in developing and transition countries
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Ezeah, Chukwunonye, Fazakerley, Jak A., and Roberts, Clive L.
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- 2013
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160. Generation mechanism of the whistler-mode waves in the plasma sheet prior to magnetic reconnection
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Wei, X.H., Cao, J.B., Zhou, G.C., Fu, H.S., Santolík, O., Rème, H., Dandouras, I., Cornilleau, N., and Fazakerley, A.
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- 2013
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161. Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2
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Humphrey, Sean J., Yang, Guang, Yang, Pengyi, Fazakerley, Daniel J., Stöckli, Jacqueline, Yang, Jean Y., and James, David E.
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- 2013
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162. Peer to Peer Support: The Disappearing Work in the Doctoral Student Experience
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Devenish, Rosemerry, Dyer, Sylvia, Jefferson, Therese, Lord, Linley, van Leeuwen, Sue, and Fazakerley, Victor
- Abstract
This paper reflects on the importance of study groups in contributing to a positive postgraduate experience and explores specific concepts, such as "disappearing behaviours" in contrasting the "official" languages of universities with the beneficial behaviours found through study groups. Additionally the paper advocates the systematic inclusion of strategies such as study groups in the postgraduate experience. Funding arrangements and research frameworks for higher education in Australia focus on student progress and completion, as well as comparison of research quality within and between universities. As a result, there is a significant emphasis on timely completion of postgraduate degrees. This paper posits that there is an apparent contradiction between the indicators of success in this area, such as completion rates and annual progress reports and the positive contribution that study groups can make to the doctoral student experience. (Contains 2 figures.)
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- 2009
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163. Highlights from the 11th ISCB Student Council Symposium 2015: Dublin, Ireland. 10 July 2015.
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Katie Wilkins, Mehedi Hassan, Margherita Francescatto, Jakob B. Jespersen, R. Gonzalo Parra, Bart Cuypers, Dan F. DeBlasio, Alexander Junge, Anupama Jigisha, Farzana Rahman, Griet Laenen, Sander Willems, Lieven Thorrez, Yves Moreau, Nagarajan Raju, Sonia P. Chothani, C. Ramakrishnan, Masakazu Sekijima, M. Michael Gromiha, Paddy J. Slator, Nigel J. Burroughs, Przemyslaw Szalaj, Zhonghui Tang, Paul J. Michalski, Oskar Luo, Xingwang Li 0004, Yijun Ruan, Dariusz Plewczynski, Giulia Fiscon, Emanuel Weitschek, Massimo Ciccozzi, Paola Bertolazzi, Giovanni Felici, Pieter Meysman, Manu Vanaerschot, Maya Berg, Hideo Imamura, Jean-Claude Dujardin, Kris Laukens, Westa Domanova, James R. Krycer, Rima Chaudhuri, Pengyi Yang, Fatemeh Vafaee, Daniel J. Fazakerley, Sean J. Humphrey, David E. James, and Zdenka Kuncic
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- 2016
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164. Programmed Cell Death in Virus Infections of the Nervous System
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Fazakerley, J. K., Allsopp, T. E., Compans, R. W., editor, Cooper, M., editor, Ito, Y., editor, Koprowski, H., editor, Melchers, F., editor, Oldstone, M., editor, Olsnes, S., editor, Potter, M., editor, Vogt, P. K., editor, Wagner, H., editor, and Gosztonyi, Georg, editor
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- 2001
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165. Entry of Viruses into the Central Nervous System
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Fazakerley, John K., Kobiler, David, editor, Lustig, Shlomo, editor, and Shapira, Shlomo, editor
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- 2001
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166. Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
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Daniel J. Fazakerley, Julian van Gerwen, Kristen C. Cooke, Xiaowen Duan, Elise J. Needham, Søren Madsen, Dougall M. Norris, Amber S. Shun-Shion, James R. Krycer, James G. Burchfield, Pengyi Yang, Mark R. Wade, Joseph T. Brozinick, David E. James, and Sean J. Humphrey
- Abstract
The failure of metabolic tissues to appropriately respond to insulin (“insulin resistance”) is an early marker in the pathogenesis of type 2 diabetes. Protein phosphorylation is central to the adipocyte insulin response, but how adipocyte signaling networks are dysregulated upon insulin resistance is unknown. Here we employed phosphoproteomics to delineate insulin signal transduction in adipocyte cells and adipose tissue. Across a range of insults triggering insulin resistance, we observed marked rewiring of the insulin signaling network. This included both attenuated insulin-responsive phosphorylation, and the emergence of phosphorylation uniquely insulin-regulated in insulin resistance. Identifying signaling changes common to multiple insults revealed subnetworks likely containing causal drivers of insulin resistance. Focusing on defective GSK3 signaling initially observed in a relatively small subset of well-characterized substrates, we employed a pipeline for identifying context-specific kinase substrates. This facilitated robust identification of widespread dysregulated GSK3 signaling. Pharmacological inhibition of GSK3 partially reversed insulin resistance in cells and tissue explants. These data highlight that insulin resistance is a multi-nodal signaling defect that encompasses dysregulated GSK3 activity.
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- 2022
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167. Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis
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Sean J. Humphrey, James G. Burchfield, Milad Ghomlaghi, David E. James, Kristen C. Cooke, Thomas A Geddes, Luke Carroll, Guang Yang, Pengyi Yang, Lan K. Nguyen, Sung-Young Shin, Daniel J. Fazakerley, Dougall M. Norris, Martin Kin Lok Wong, Alison L Kearney, Kearney, Alison L [0000-0002-5736-3393], Ghomlaghi, Milad [0000-0001-9047-1049], Nguyen, Lan K [0000-0003-4040-7705], James, David E [0000-0001-5946-5257], Burchfield, James G [0000-0002-6609-6151], Apollo - University of Cambridge Repository, Humphrey, Sean J [0000-0002-2666-9744], and Fazakerley, Daniel [0000-0001-8241-2903]
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0301 basic medicine ,Mouse ,medicine.medical_treatment ,plasma membrane ,PI3K ,Mice ,Phosphatidylinositol 3-Kinases ,computational biology ,0302 clinical medicine ,Insulin receptor substrate ,Biology (General) ,biology ,Chemistry ,phosphorylation ,General Neuroscience ,systems biology ,General Medicine ,Cell biology ,030220 oncology & carcinogenesis ,Medicine ,Signal transduction ,signal transduction ,Research Article ,Computational and Systems Biology ,Human ,Cell signaling ,insulin ,QH301-705.5 ,Science ,Mechanistic Target of Rapamycin Complex 1 ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Antigens, CD ,Negative feedback ,medicine ,Animals ,Humans ,Protein kinase B ,PI3K/AKT/mTOR pathway ,General Immunology and Microbiology ,Insulin ,Akt ,Cell Membrane ,Cell Biology ,Receptor, Insulin ,Insulin receptor ,Glucose ,030104 developmental biology ,Insulin Receptor Substrate Proteins ,biology.protein ,Phosphatidylinositol 3-Kinase ,Proto-Oncogene Proteins c-akt - Abstract
The phosphoinositide 3-kinase (PI3K)-Akt network is tightly controlled by feedback mechanisms that regulate signal flow and ensure signal fidelity. A rapid overshoot in insulin-stimulated recruitment of Akt to the plasma membrane has previously been reported, which is indicative of negative feedback operating on acute timescales. Here, we show that Akt itself engages this negative feedback by phosphorylating insulin receptor substrate (IRS) 1 and 2 on a number of residues. Phosphorylation results in the depletion of plasma membrane-localised IRS1/2, reducing the pool available for interaction with the insulin receptor. Together these events limit plasma membrane-associated PI3K and phosphatidylinositol (3,4,5)-trisphosphate (PIP3) synthesis. We identified two Akt-dependent phosphorylation sites in IRS2 at S306 (S303 in mouse) and S577 (S573 in mouse) that are key drivers of this negative feedback. These findings establish a novel mechanism by which the kinase Akt acutely controls PIP3 abundance, through post-translational modification of the IRS scaffold., eLife digest For the body to work properly, cells must constantly ‘talk’ to each other using signalling molecules. Receiving a chemical signal triggers a series of molecular events in a cell, a so-called ‘signal transduction pathway’ that connects a signal with a precise outcome. Disturbing cell signalling can trigger disease, and strict control mechanisms are therefore in place to ensure that communication does not break down or become erratic. For instance, just as a thermostat turns off the heater once the right temperature is reached, negative feedback mechanisms in cells switch off signal transduction pathways when the desired outcome has been achieved. The hormone insulin is a signal for growth that increases in the body following a meal to promote the storage of excess blood glucose (sugar) in muscle and fat cells. The hormone binds to insulin receptors at the cell surface and switches on a signal transduction pathway that makes the cell take up glucose from the bloodstream. If the signal is not engaged diseases such as diabetes develop. Conversely, if the signal cannot be adequately switched of cancer can develop. Determining exactly how insulin works would help to understand these diseases better and to develop new treatments. Kearney et al. therefore set out to examine the biochemical ‘fail-safes’ that control insulin signalling. Experiments using computer simulations of the insulin signalling pathway revealed a potential new mechanism for negative feedback, which centred on a molecule known as Akt. The models predicted that if the negative feedback were removed, then Akt would become hyperactive and accumulate at the cell’s surface after stimulation with insulin. Further manipulation of the ‘virtual’ insulin signalling pathway and studies of live cells in culture confirmed that this was indeed the case. The cell biology experiments also showed how Akt, once at the cell surface, was able to engage the negative feedback and shut down further insulin signalling. Akt did this by inactivating a protein required to pass the signal from the insulin receptor to the rest of the cell. Overall, this work helps to understand cell communication by revealing a previously unknown, and critical component of the insulin signalling pathway.
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- 2021
168. Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate
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Duan, X, Norris, DM, Humphrey, SJ, Yang, P, Cooke, KC, Bultitude, WP, Parker, BL, Conway, OJ, Burchfield, JG, Krycer, JR, Brodsky, FM, James, DE, Fazakerley, DJ, Duan, X, Norris, DM, Humphrey, SJ, Yang, P, Cooke, KC, Bultitude, WP, Parker, BL, Conway, OJ, Burchfield, JG, Krycer, JR, Brodsky, FM, James, DE, and Fazakerley, DJ
- Abstract
Trafficking regulator of GLUT4-1, TRARG1, positively regulates insulin-stimulated GLUT4 trafficking and insulin sensitivity. However, the mechanism(s) by which this occurs remain(s) unclear. Using biochemical and mass spectrometry analyses we found that TRARG1 is dephosphorylated in response to insulin in a PI3K/Akt-dependent manner and is a novel substrate for GSK3. Priming phosphorylation of murine TRARG1 at serine 84 allows for GSK3-directed phosphorylation at serines 72, 76 and 80. A similar pattern of phosphorylation was observed in human TRARG1, suggesting that our findings are translatable to human TRARG1. Pharmacological inhibition of GSK3 increased cell surface GLUT4 in cells stimulated with a submaximal insulin dose, and this was impaired following Trarg1 knockdown, suggesting that TRARG1 acts as a GSK3-mediated regulator in GLUT4 trafficking. These data place TRARG1 within the insulin signaling network and provide insights into how GSK3 regulates GLUT4 trafficking in adipocytes.
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- 2022
169. Suppressor of Cytokine Signalling 5 (SOCS5) Modulates Inflammatory Responses during Alphavirus Infection
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Kedzierski, L, Tan, AEQ, Foo, IJH, Nicholson, SE, Fazakerley, JK, Kedzierski, L, Tan, AEQ, Foo, IJH, Nicholson, SE, and Fazakerley, JK
- Abstract
CNS viral infections are one of the major causes of morbidity and mortality worldwide and a significant global public health concern. Uncontrolled inflammation and immune responses in the brain, despite their protective roles, can also be harmful. The suppressor of cytokine signalling (SOCS) proteins is one of the key mechanisms controlling inflammatory and immune responses across all tissues including the brain. SOCS5 is highly expressed in the brain but there is little understanding of its role in the CNS. Using a mouse model of encephalitis, we demonstrate that lack of SOCS5 results in changes in the pathogenesis and clinical outcome of a neurotropic virus infection. Relative to wild-type mice, SOCS5-deficient mice had greater weight loss, dysregulated cytokine production and increased neuroinflammatory infiltrates composed predominantly of CD11b+ cells. We conclude that in the brain, SOCS5 is a vital regulator of anti-viral immunity that mediates the critical balance between immunopathology and virus persistence.
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- 2022
170. Stability-based assessment of Monteggia-type injuries predicts failure of treatment
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Walton, Roger D.M., Ormsby, Neal M., Brookes-Fazakerley, Steven D., Manohar, Shambavi, Patel, Trupesh, Wright, David M., Bass, Alf A., and Bruce, Colin E.
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- 2017
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171. The amino acid transporter, SLC1A3, is plasma membrane‐localised in adipocytes and its activity is insensitive to insulin
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Krycer, James R., Fazakerley, Daniel J., Cater, Rosemary J., C. Thomas, Kristen, Naghiloo, Sheyda, Burchfield, James G., Humphrey, Sean J., Vandenberg, Robert J., Ryan, Renae M., and James, David E.
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- 2017
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172. Characteristics of the Taylor microscale in the solar wind/foreshock: magnetic field and electron velocity measurements
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C. Gurgiolo, M. L. Goldstein, W. H. Matthaeus, A. Viñas, and A. N. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The Taylor microscale is one of the fundamental turbulence scales. Not easily estimated in the interplanetary medium employing single spacecraft data, it has generally been studied through two point correlations. In this paper we present an alternative, albeit mathematically equivalent, method for estimating the Taylor microscale (λT). We make two independent determinations employing multi-spacecraft data sets from the Cluster mission, one using magnetic field data and a second using electron velocity data. Our results using the magnetic field data set yields a scale length of 1538 ± 550 km, slightly less than, but within the same range as, values found in previous magnetic-field-based studies. During time periods where both magnetic field and electron velocity data can be used, the two values can be compared. Relative comparisons show λT computed from the velocity is often significantly smaller than that from the magnetic field data. Due to a lack of events where both measurements are available, the absolute λT based on the electron fluid velocity is not able to be determined.
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- 2013
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173. Double cusp encounter by Cluster: double cusp or motion of the cusp?
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C. P. Escoubet, J. Berchem, K. J. Trattner, F. Pitout, R. Richard, M. G. G. T. Taylor, J. Soucek, B. Grison, H. Laakso, A. Masson, M. Dunlop, I. Dandouras, H. Reme, A. Fazakerley, and P. Daly
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Modelling plasma entry in the polar cusp has been successful in reproducing ion dispersions observed in the cusp at low and mid-altitudes. The use of a realistic convection pattern, when the IMF-By is large and stable, allowed Wing et al. (2001) to predict double cusp signatures that were subsequently observed by the DMSP spacecraft. In this paper we present a cusp crossing where two cusp populations are observed, separated by a gap around 1° Invariant Latitude (ILAT) wide. Cluster 1 (C1) and Cluster 2 (C2) observed these two cusp populations with a time delay of 3 min, and about 15 and 42 min later Cluster 4 (C4) and Cluster 3 (C3) observed, respectively, a single cusp population. A peculiarity of this event is the fact that the second cusp population seen on C1 and C2 was observed at the same time as the first cusp population on C4. This would tend to suggest that the two cusp populations had spatial features similar to the double cusp. Due to the nested crossing of C1 and C2 through the gap between the two cusp populations, C2 being first to leave the cusp and last to re-enter it, these observations are difficult to be explained by two distinct cusps with a gap in between. However, since we observe the cusp in a narrow area of local time post-noon, a second cusp may have been present in the pre-noon sector but could not be observed. On the other hand, these observations are in agreement with a motion of the cusp first dawnward and then back duskward due to the effect of the IMF-By component.
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- 2013
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174. The Earth: Plasma Sources, Losses, and Transport Processes
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Welling, Daniel T., André, Mats, Dandouras, Iannis, Delcourt, Dominique, Fazakerley, Andrew, Fontaine, Dominique, Foster, John, Ilie, Raluca, Kistler, Lynn, Lee, Justin H., Liemohn, Michael W., Slavin, James A., Wang, Chih-Ping, Wiltberger, Michael, and Yau, Andrew
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- 2015
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175. Insulin regulates Rab3–Noc2 complex dissociation to promote GLUT4 translocation in rat adipocytes
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Koumanov, Francoise, Pereira, Vinit J., Richardson, Judith D., Sargent, Samantha L., Fazakerley, Daniel J., and Holman, Geoffrey D.
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- 2015
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176. Detection and identification of putative bacterial endosymbionts and endogenous viruses in tick cell lines
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Alberdi, M. Pilar, Dalby, Matthew J., Rodriguez-Andres, Julio, Fazakerley, John K., Kohl, Alain, and Bell-Sakyi, Lesley
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- 2012
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177. Immature Brain Cortical Neurons Have Low Transcriptional Competence to Activate Antiviral Defences and Control RNA Virus Infections
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Narayanan, Divya, primary, Moily, Nagaraj, additional, McQuilten, Hayley A., additional, Kedzierska, Katherine, additional, Mackenzie, Jason M., additional, Kedzierski, Lukasz, additional, and Fazakerley, John K., additional
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- 2022
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178. Trafficking regulator of GLUT4-1 (TRARG1) is a GSK3 substrate
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Duan, Xiaowen, primary, Norris, Dougall M., additional, Humphrey, Sean J., additional, Yang, Pengyi, additional, Cooke, Kristen C., additional, Bultitude, Will P., additional, Parker, Benjamin L., additional, Conway, Olivia J., additional, Burchfield, James G., additional, Krycer, James R., additional, Brodsky, Frances M., additional, James, David E., additional, and Fazakerley, Daniel J., additional
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- 2022
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179. Integrating adipocyte insulin signaling and metabolism in the multi-omics era
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Calejman, C. Martinez, primary, Doxsey, W.G., additional, Fazakerley, D.J., additional, and Guertin, D.A., additional
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- 2022
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180. The exocyst complex is an essential component of the mammalian constitutive secretory pathway
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Pereira, Conceição, primary, Stalder, Danièle, additional, Anderson, Georgina, additional, Shun-Shion, Amber S., additional, Houghton, Jack, additional, Antrobus, Robin, additional, Chapman, Michael A., additional, Fazakerley, Daniel J., additional, and Gershlick, David C, additional
- Published
- 2022
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181. Phosphoproteomics reveals rewiring of the insulin signaling network and multi-nodal defects in insulin resistance
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Fazakerley, Daniel J., primary, van Gerwen, Julian, additional, Cooke, Kristen C., additional, Duan, Xiaowen, additional, Needham, Elise J., additional, Madsen, Søren, additional, Norris, Dougall M., additional, Shun-Shion, Amber S., additional, Krycer, James R., additional, Burchfield, James G., additional, Yang, Pengyi, additional, Wade, Mark R., additional, Brozinick, Joseph T., additional, James, David E., additional, and Humphrey, Sean J., additional
- Published
- 2022
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182. The Earth: Plasma Sources, Losses, and Transport Processes
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Welling, Daniel T., primary, André, Mats, additional, Dandouras, Iannis, additional, Delcourt, Dominique, additional, Fazakerley, Andrew, additional, Fontaine, Dominique, additional, Foster, John, additional, Ilie, Raluca, additional, Kistler, Lynn, additional, Lee, Justin H., additional, Liemohn, Michael W., additional, Slavin, James A., additional, Wang, Chih-Ping, additional, Wiltberger, Michael, additional, and Yau, Andrew, additional
- Published
- 2016
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183. Encephalitic Alphaviruses
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McKimmie, Clive S., primary and Fazakerley, John K., additional
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- 2016
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184. Unraveling Kinase Activation Dynamics Using Kinase-Substrate Relationships from Temporal Large-Scale Phosphoproteomics Studies.
- Author
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Westa Domanova, James Krycer, Rima Chaudhuri, Pengyi Yang, Fatemeh Vafaee, Daniel Fazakerley, Sean Humphrey, David James, and Zdenka Kuncic
- Subjects
Medicine ,Science - Abstract
In response to stimuli, biological processes are tightly controlled by dynamic cellular signaling mechanisms. Reversible protein phosphorylation occurs on rapid time-scales (milliseconds to seconds), making it an ideal carrier of these signals. Advances in mass spectrometry-based proteomics have led to the identification of many tens of thousands of phosphorylation sites, yet for the majority of these the kinase is unknown and the underlying network topology of signaling networks therefore remains obscured. Identifying kinase substrate relationships (KSRs) is therefore an important goal in cell signaling research. Existing consensus sequence motif based prediction algorithms do not consider the biological context of KSRs, and are therefore insensitive to many other mechanisms guiding kinase-substrate recognition in cellular contexts. Here, we use temporal information to identify biologically relevant KSRs from Large-scale In Vivo Experiments (KSR-LIVE) in a data-dependent and automated fashion. First, we used available phosphorylation databases to construct a repository of existing experimentally-predicted KSRs. For each kinase in this database, we used time-resolved phosphoproteomics data to examine how its substrates changed in phosphorylation over time. Although substrates for a particular kinase clustered together, they often exhibited a different temporal pattern to the phosphorylation of the kinase. Therefore, although phosphorylation regulates kinase activity, our findings imply that substrate phosphorylation likely serve as a better proxy for kinase activity than kinase phosphorylation. KSR-LIVE can thereby infer which kinases are regulated within a biological context. Moreover, KSR-LIVE can also be used to automatically generate positive training sets for the subsequent prediction of novel KSRs using machine learning approaches. We demonstrate that this approach can distinguish between Akt and Rps6kb1, two kinases that share the same linear consensus motif, and provide evidence suggesting IRS-1 S265 as a novel Akt site. KSR-LIVE is an open-access algorithm that allows users to dissect phosphorylation signaling within a specific biological context, with the potential to be included in the standard analysis workflow for studying temporal high-throughput signal transduction data.
- Published
- 2016
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185. Lactate production is a prioritized feature of adipocyte metabolism
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Deanne Francis, Xiaowen Duan, Akiyoshi Hirayama, Yushi Kamei, Sergey Kurdyukov, Lake-Ee Quek, Sarah D. Elkington, Satsuki Ikeda, David E. James, James R. Krycer, Alexis Díaz-Vegas, Fiona Weiss, Tomoyoshi Soga, Uttam K. Tambar, Kristen C. Cooke, Daniel J. Fazakerley, Ping Xin Zhou, and Gregory J. Cooney
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Glucose uptake ,Fat Body ,Adipose tissue ,Carbohydrate metabolism ,Biochemistry ,Rats, Sprague-Dawley ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Adipocyte ,Internal medicine ,Lactate dehydrogenase ,Adipocytes ,medicine ,Animals ,Homeostasis ,Insulin ,Glucose homeostasis ,Lactic Acid ,Molecular Biology ,Cells, Cultured ,030102 biochemistry & molecular biology ,3T3 Cells ,Cell Biology ,Metabolism ,medicine.disease ,Rats ,Glucose ,030104 developmental biology ,Endocrinology ,chemistry ,Drosophila - Abstract
Adipose tissue is essential for whole-body glucose homeostasis, with a primary role in lipid storage. It has been previously observed that lactate production is also an important metabolic feature of adipocytes, but its relationship to adipose and whole-body glucose disposal remains unclear. Therefore, using a combination of metabolic labeling techniques, here we closely examined lactate production of cultured and primary mammalian adipocytes. Insulin treatment increased glucose uptake and conversion to lactate, with the latter responding more to insulin than did other metabolic fates of glucose. However, lactate production did not just serve as a mechanism to dispose of excess glucose, because we also observed that lactate production in adipocytes did not solely depend on glucose availability and even occurred independently of glucose metabolism. This suggests that lactate production is prioritized in adipocytes. Furthermore, knocking down lactate dehydrogenase specifically in the fat body of Drosophila flies lowered circulating lactate and improved whole-body glucose disposal. These results emphasize that lactate production is an additional metabolic role of adipose tissue beyond lipid storage and release.
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- 2020
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186. Mitochondrial oxidants, but not respiration, are sensitive to glucose in adipocytes
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Daniel J. Fazakerley, Alexis Díaz-Vegas, Sarah D. Elkington, Gregory J. Cooney, David E. James, Kelsey H. Fisher-Wellman, James G. Burchfield, James R. Krycer, and Kristen C. Cooke
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Male ,0301 basic medicine ,medicine.medical_specialty ,Glucose uptake ,medicine.medical_treatment ,Cell Respiration ,Adipose tissue ,Type 2 diabetes ,Bioenergetics ,Mitochondrion ,Biochemistry ,Rats, Sprague-Dawley ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Internal medicine ,Adipocyte ,Adipocytes ,medicine ,Animals ,Insulin ,Glucose homeostasis ,Molecular Biology ,Cells, Cultured ,030102 biochemistry & molecular biology ,Chemistry ,3T3 Cells ,Cell Biology ,medicine.disease ,Mitochondria ,Rats ,Oxygen ,Oxidative Stress ,Glucose ,030104 developmental biology ,Endocrinology ,Insulin Resistance - Abstract
Insulin action in adipose tissue is crucial for whole-body glucose homeostasis, with insulin resistance being a major risk factor for metabolic diseases such as type 2 diabetes. Recent studies have proposed mitochondrial oxidants as a unifying driver of adipose insulin resistance, serving as a signal of nutrient excess. However, neither the substrates for nor sites of oxidant production are known. Because insulin stimulates glucose utilization, we hypothesized that glucose oxidation would fuel respiration, in turn generating mitochondrial oxidants. This would impair insulin action, limiting further glucose uptake in a negative feedback loop of “glucose-dependent” insulin resistance. Using primary rat adipocytes and cultured 3T3-L1 adipocytes, we observed that insulin increased respiration, but notably this occurred independently of glucose supply. In contrast, glucose was required for insulin to increase mitochondrial oxidants. Despite rising to similar levels as when treated with other agents that cause insulin resistance, glucose-dependent mitochondrial oxidants failed to cause insulin resistance. Subsequent studies revealed a temporal relationship whereby mitochondrial oxidants needed to increase before the insulin stimulus to induce insulin resistance. Together, these data reveal that (a) adipocyte respiration is principally fueled from nonglucose sources; (b) there is a disconnect between respiration and oxidative stress, whereby mitochondrial oxidant levels do not rise with increased respiration unless glucose is present; and (c) mitochondrial oxidative stress must precede the insulin stimulus to cause insulin resistance, explaining why short-term, insulin-dependent glucose utilization does not promote insulin resistance. These data provide additional clues to mechanistically link nutrient excess to adipose insulin resistance.
- Published
- 2020
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187. Flux Transfer Events in the Northern Hemisphere Polar Cusp Under Strong IMF Bx
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Caoimhe Doherty, Andrew Fazakerley, Christopher Owen, Robert Fear, Colin Forsyth, Andrew Kavanagh, Karl-Heinz Trattner, and Yulia Bogdanova
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Physics::Space Physics - Abstract
The location, shape, and size of the magnetospheric polar cusps are heavily influenced by upstream solar wind conditions. The effects of dominant IMF Bz and By components on the cusp are now well known. However, the effect of a strong IMF Bx component on the structure of the polar cusps is relatively unexplored. We present a case study of data recorded by the four Cluster spacecraft during a crossing of the northern hemisphere high altitude cusp in the winter season of 2018, when the IMF is directed southward and sunward. The Cluster spacecraft traverse the high-altitude cusp with separations between several hundred km and 1.5 Earth radii between each spacecraft, and travel at a roughly constant latitude with changing MLT. We study these observations in conjunction with those of the ground based SuperDARN radars.Each spacecraft observes many flux transfer type events within the cusp, although some events are not seen on all 4 spacecraft. The magnetic field orientation often varies significantly during each distinct passage through individual flux tubes, clearly departing from the background magnetic field direction expected in the northern hemisphere high altitude cusp. A number of these events show bidirectional electron flux signatures typical of those expected on recently reconnected open northern hemisphere flux tubes. However, some flux tubes appear to be populated only by antiparallel moving electrons, while others show an isotropic distribution of electrons and ions. The SuperDARN STO radar site observes Poleward Moving Auroral Forms (PMAFs), consistent with the interpretation that Cluster observes open flux tubes, however the directions of convecting flux tubes seen by Cluster are not always consistent with the SuperDARN picture. We consider whether the influence of the strong IMF Bx results in the relocation of the dayside reconnection site to high northern latitudes, allowing Cluster to encounter a mix of open flux tubes in the northern cusp, each of which may be connected to either the Northern or Southern polar ionosphere. The latter configuration may be particularly supported if reconnection near the cusp results in southern hemisphere open field lines being driven anti-sunward into the northern cusp as a result of enhanced sheath flows overcoming their magnetic tension at these latitudes.
- Published
- 2022
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188. Electrochemical synthesis of isoxazolines : method and mechanism
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Samuel D. L. Holman, Alfie G. Wills, Neal J. Fazakerley, Darren L. Poole, Diane M. Coe, Leonard A. Berlouis, and Marc Reid
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Cycloaddition Reaction ,Oximes ,Organic Chemistry ,Electrons ,QD ,Isoxazoles ,General Chemistry ,Alkenes ,Catalysis - Abstract
An electrochemical method for the green and practical synthesis of a broad range of substituted isoxazoline cores is presented. Both aryl and more challenging alkyl aldoximes are converted to the desired isoxazoline in an electrochemically enabled regio- and diastereoselective reaction with electron-deficient alkenes. Additionally, in-situ reaction monitoring methods compatible with electrochemistry equipment have been developed in order to probe the reaction pathway. Supporting analyses from kinetic (time-course) modelling and density functional theory support a stepwise, radical-mediated mechanism, and discounts hypothesised involvement of closed shell [3+2] cycloaddition pathways.
- Published
- 2022
189. The Stability of the Electron Strahl against the Oblique Fast-magnetosonic/Whistler Instability in the Inner Heliosphere
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Seong-Yeop Jeong, Joel B. Abraham, Daniel Verscharen, Laura Berčič, David Stansby, Georgios Nicolaou, Christopher J. Owen, Robert T. Wicks, Andrew N. Fazakerley, Jeffersson A. Agudelo Rueda, and Mayur Bakrania
- Subjects
Plasma Physics (physics.plasm-ph) ,Astrophysics - Solar and Stellar Astrophysics ,Physics - Space Physics ,F300 ,Space and Planetary Science ,Physics::Space Physics ,FOS: Physical sciences ,Astrophysics::Solar and Stellar Astrophysics ,Astronomy and Astrophysics ,F500 ,Solar and Stellar Astrophysics (astro-ph.SR) ,Space Physics (physics.space-ph) ,Physics - Plasma Physics - Abstract
We analyze the micro-kinetic stability of the electron strahl in the solar wind depending on heliocentric distance. The oblique fast-magnetosonic/whistler (FM/W) instability has emerged in the literature as a key candidate mechanism for the effective scattering of the electron strahl into the electron halo population. Using data from Parker Solar Probe (PSP) and Helios, we compare the measured strahl properties with the analytical thresholds for the oblique FM/W instability in the low- and high-$\beta_{\parallel c}$ regimes, where $\beta_{\parallel c}$ is the ratio of the core parallel thermal pressure to the magnetic pressure. Our PSP and Helios data show that the electron strahl is on average stable against the oblique FM/W instability in the inner heliosphere. Our analysis suggests that the instability, if at all, can only be excited sporadically and on short timescales. We discuss the caveats of our analysis and potential alternative explanations for the observed scattering of the electron strahl in the solar wind. Furthermore, we recommend the numerical evaluation of the stability of individual distributions in the future to account for any uncertainties in the validity of the analytical expressions for the instability thresholds., Comment: Accepted in ApJL
- Published
- 2022
190. Spatial distribution of rolled up Kelvin-Helmholtz vortices at Earth's dayside and flank magnetopause
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M. G. G. T. Taylor, H. Hasegawa, B. Lavraud, T. Phan, C. P. Escoubet, M. W. Dunlop, Y. V. Bogdanova, A. L. Borg, M. Volwerk, J. Berchem, O. D. Constantinescu, J. P. Eastwood, A. Masson, H. Laakso, J. Soucek, A. N. Fazakerley, H. U. Frey, E. V. Panov, C. Shen, J. K. Shi, D. G. Sibeck, Z. Y. Pu, J. Wang, and J. A. Wild
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The Kelvin-Helmholtz Instability (KHI) can drive waves at the magnetopause. These waves can grow to form rolled-up vortices and facilitate transfer of plasma into the magnetosphere. To investigate the persistence and frequency of such waves at the magnetopause we have carried out a survey of all Double Star 1 magnetopause crossings, using a combination of ion and magnetic field measurements. Using criteria originally used in a Geotail study made by Hasegawa et al. (2006) (forthwith referred to as H2006), 17 candidate events were identified from the entire TC-1 mission (covering ~623 orbits where the magnetopause was sampled), a majority of which were on the dayside of the terminator. The relationship between density and shear velocity was then investigated, to identify the predicted signature of a rolled up vortex from H2006 and all 17 events exhibited some level of rolled up behavior. The location of the events had a clear dawn-dusk asymmetry, with 12 (71%) on the post noon, dusk flank suggesting preferential growth in this region.
- Published
- 2012
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191. Cluster and TC-1 observation of magnetic holes in the plasma sheet
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W. J. Sun, Q. Q. Shi, S. Y. Fu, Z. Y. Pu, M. W. Dunlop, A. P. Walsh, Q. G. Zong, T. Xiao, C. L. Tang, H. Reme, C. Carr, E. Lucek, and A. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Magnetic holes with relatively small scale sizes, detected by Cluster and TC-1 in the magnetotail plasma sheet, are studied in this paper. It is found that these magnetic holes are spatial structures and they are not magnetic depressions generated by the flapping movement of the magnetotail current sheet. Most of the magnetic holes (93%) were observed during intervals with Bz larger than Bx, i.e. they are more likely to occur in a dipolarized magnetic field topology. Our results also suggest that the occurrence of these magnetic holes might have a close relationship with the dipolarization process. The magnetic holes typically have a scale size comparable to the local proton Larmor radius and are accompanied by an electron energy flux enhancement at a 90° pitch angle, which is quite different from the previously observed isotropic electron distributions inside magnetic holes in the plasma sheet. It is also shown that most of the magnetic holes occur in marginally mirror-stable environments. Whether the plasma sheet magnetic holes are generated by the mirror instability related to ions or not, however, is unknown. Comparison of ratios, scale sizes and propagation direction of magnetic holes detected by Cluster and TC-1, suggests that magnetic holes observed in the vicinity of the TC-1 orbit (~7–12 RE) are likely to be further developed than those observed by Cluster (~7–18 RE).
- Published
- 2012
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192. Direct observations of the formation of the solar wind halo from the strahl
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C. Gurgiolo, M. L. Goldstein, A. F. Viñas, and A. N. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Observations of a continual erosion of the strahl and build up of the halo with distance from the sun suggests that, at least in part, the halo may be formed as a result of scattering of the strahl. This hypothesis is supported in this paper by observation of intense scattering of strahl electrons, which gives rise to a proto-halo electron population. This population eventually merges into, or becomes the halo. The fact that observations of intense scattering of the strahl are not common implies that the formation of the halo may not be a continuous process, but one that occurs, in part, in bursts in regions where the conditions responsible for the scattering are optimum.
- Published
- 2012
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193. Inter-hemispheric asymmetry of dependence of the cusp location on dipole tilt during northward IMF conditions
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J. Shi, J. Guo, M. Dunlop, T. Zhang, Z. Liu, E. Lucek, A. Fazakerley, H. Rème, and I. Dandouras
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
A data set of the Cluster cusp crossings over a 5-year period is studied for the interhemispheric comparison of the dipole tilt angle effect on the latitude of the mid-altitude cusp. The result shows that the dipole tilt angle has a clear control of the cusp latitudinal location. Although, the northern cusp moves 0.054° ILAT for every 1° increase in the dipole tilt angle at the mean altitude of 5.2 RE, the southern cusp moves 0.051° ILAT for every 1° increase in the dipole tilt angle at the mean altitude of 6.6 RE. The northern cusp dependence agrees with the trend formed by other observations of different satellites for different altitudes, whereas the southern cusp does not. We therefore suggest that there is an inter-hemispheric difference in the dipole tilt angle dependence of cusp, latitudinal location, which has an impact on other observations of different satellites in different altitudes.
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- 2012
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194. Proton/electron temperature ratio in the magnetotail
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A. V. Artemyev, W. Baumjohann, A. A. Petrukovich, R. Nakamura, I. Dandouras, and A. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
We study the temperature ratio of protons and electrons based on statistics of Cluster observations in the Earth's magnetotail from 2001 to 2004. During these years, from June to November, the Cluster spacecraft visited the plasma sheet at the distance r~−19 RE. We use proton and electron moments collected in the central region of the plasma sheet (|Z|RE). We find an average ratio of proton and electron temperatures ⟨Tp/Te ⟩ ≈ 3.5. The dependence of Tp on Te can be approximated by the power-law function: Tp ≈ 11Te0.62, where Tp and Te are measured in MK. A previous reported ratio ⟨Tp/Te ⟩ ~ 7 is limited to small electron temperatures Te and curlometer current density is not found.
- Published
- 2011
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195. Cluster observations of a transient signature in the magnetotail: implications for the mode of reconnection
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S. Beyene, C. J. Owen, A. P. Walsh, C. Forsyth, A. N. Fazakerley, S. Kiehas, I. Dandouras, and E. Lucek
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
Travelling compression regions (TCRs) are perturbations in the magnetotail lobe magnetic field caused by structures moving Earthward or tailward within the plasma sheet. Previous works have suggested that these structures are created by either time-dependant reconnection occurring at a single X-line, forming a flux-bulge-type structure, or space-variant reconnection at multiple X-lines, forming flux-rope-type structures. In this study we examine an event in which Cluster 2 observed a TCR while the 3 remaining Cluster spacecraft observed the underlying magnetic structure at a range of distances from the neutral sheet. The magnetic structure has a velocity of (99, 154, −31) km s−1 in GSM (|V| = 186 km s−1), an estimated size of 1.19 RE along the direction of travel and a size between 1.94 and 2.86 RE in the direction perpendicular to the current sheet. As the structure passes the spacecraft, Cluster 1 and Cluster 4 observed a bipolar signature in BZ, plasma-sheet-like plasma and field-aligned electron flows. Cluster 3 passed closest to the centre of the structure and observed two separate reductions in the plasma density (with field-aligned electron flows); these drop-outs in the plasma sheet were possibly created by the actions of X-lines. The second drop-out in the plasma sheet also includes a reversal of the ion flow, a signature consistent with the passage of a reconnecting X-line past the spacecraft. Between the X-lines, the plasma outflow from the X-lines caused an increase in pressure which led to a localised expansion of the plasma and also the observations at Cluster 1 and Cluster 4 and the TCR. Our observations do not uniquely match either of the flux rope or the flux bulge predictions although the observation of two plasma sheet drop-outs (interpreted as X-lines, one active, one dormant) with plasma-sheet-like between them and only one TCR is a situation expected in multiple X-line reconnection.
- Published
- 2011
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196. Observations of electron vorticity in the inner plasma sheet
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C. Gurgiolo, M. L. Goldstein, A. F. Viñas, W. H. Matthaeus, and A. N. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
From a limited number of observations it appears that vorticity is a common feature in the inner plasma sheet. With the four Cluster spacecraft and the four PEACE instruments positioned in a tetrahedral configuration, for the first time it is possible to directly estimate the electron fluid vorticity in a space plasma. We show examples of electron fluid vorticity from multiple plasma sheet crossings. These include three time periods when Cluster passed through a reconnection ion diffusion region. Enhancements in vorticity are seen in association with each crossing of the ion diffusion region.
- Published
- 2011
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197. Interplanetary magnetic field rotations followed from L1 to the ground: the response of the Earth's magnetosphere as seen by multi-spacecraft and ground-based observations
- Author
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M. Volwerk, J. Berchem, Y. V. Bogdanova, O. D. Constantinescu, M. W. Dunlop, J. P. Eastwood, P. Escoubet, A. N. Fazakerley, H. Frey, H. Hasegawa, B. Lavraud, E. V. Panov, C. Shen, J. K. Shi, M. G. G. T. Taylor, J. Wang, J. A. Wild, Q. H. Zhang, O. Amm, and J. M. Weygand
- Subjects
Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
A study of the interaction of solar wind magnetic field rotations with the Earth's magnetosphere is performed. For this event there is, for the first time, a full coverage over the dayside magnetosphere with multiple (multi)spacecraft missions from dawn to dusk, combined with ground magnetometers, radar and an auroral camera, this gives a unique coverage of the response of the Earth's magnetosphere. After a long period of southward IMF Bz and high dynamic pressure of the solar wind, the Earth's magnetosphere is eroded and compressed and reacts quickly to the turning of the magnetic field. We use data from the solar wind monitors ACE and Wind and from magnetospheric missions Cluster, THEMIS, DoubleStar and Geotail to investigate the behaviour of the magnetic rotations as they move through the bow shock and magnetosheath. The response of the magnetosphere is investigated through ground magnetometers and auroral keograms. It is found that the solar wind magnetic field drapes over the magnetopause, while still co-moving with the plasma flow at the flanks. The magnetopause reacts quickly to IMF Bz changes, setting up field aligned currents, poleward moving aurorae and strong ionospheric convection. Timing of the structures between the solar wind, magnetosheath and the ground shows that the advection time of the structures, using the solar wind velocity, correlates well with the timing differences between the spacecraft. The reaction time of the magnetopause and the ionospheric current systems to changes in the magnetosheath Bz seem to be almost immediate, allowing for the advection of the structure measured by the spacecraft closest to the magnetopause.
- Published
- 2011
- Full Text
- View/download PDF
198. Magnetopause reconnection across wide local time
- Author
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M. W. Dunlop, Q.-H. Zhang, Y. V. Bogdanova, K. J. Trattner, Z. Pu, H. Hasegawa, J. Berchem, M. G. G. T. Taylor, M. Volwerk, J. P. Eastwood, B. Lavraud, C. Shen, J.-K. Shi, J. Wang, D. Constantinescu, A. N. Fazakerley, H. Frey, D. Sibeck, P. Escoubet, J. A. Wild, Z. X. Liu, and C. Carr
- Subjects
Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
During April to July 2007 a combination of 10 spacecraft provided simultaneous monitoring of the dayside magnetopause across a wide range of local times. The array of four Cluster spacecraft, separated at large distances (10 000 km), were traversing the dawn-side magnetopause at high and low latitudes; the five THEMIS spacecraft were often in a 4 + 1 grouped configuration, traversing the low latitude, dusk-side magnetosphere, and the Double star, TC-1 spacecraft was in an equatorial orbit between the local times of the THEMIS and Cluster orbits. We show here a number of near simultaneous conjunctions of all 10 spacecraft at the magnetopause. One conjunction identifies an extended magnetic reconnection X-line, tilted in the low latitude, sub-solar region, which exists together with active anti-parallel reconnection sites extending to locations on the dawn-side flank. Oppositely moving FTE's are observed on all spacecraft, consistent with the initially strong IMF By conditions and the comparative locations of the spacecraft both dusk-ward and dawn-ward of noon. Comparison with other conjunctions of magnetopause crossings, which are also distributed over wide local times, supports the result that reconnection activity may occur at many sites simultaneously across the sub-solar and flank magnetopause, but linked to the large scale (extended) configuration of the merging line; broadly depending on IMF orientation. The occurrence of MR therefore inherently follows a "component" driven scenario irrespective of the guide field conditions. Some conjunctions allow the global magnetopause response to IMF changes to be observed and the distribution of spacecraft can directly confirm its shape, motion and deformation at local noon, dawn and dusk-side, simultaneously.
- Published
- 2011
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199. Peace: A Plasma Electron and Current Experiment
- Author
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Johnstone, A. D., Alsop, C., Burge, S., Carter, P. J., Coates, A. J., Coker, A. J., Fazakerley, A. N., Grande, M., Gowen, R. A., Gurgiolo, C., Hancock, B. K., Narheim, B., Preece, A., Sheather, P. H., Winningham, J. D., Woodliffe, R. D., Escoubet, C. P., editor, Russell, C. T., editor, and Schmidt, R., editor
- Published
- 1997
- Full Text
- View/download PDF
200. First measurements of electron vorticity in the foreshock and solar wind
- Author
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C. Gurgiolo, M. L. Goldstein, A. F. Viñas, and A. N. Fazakerley
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Science ,Physics ,QC1-999 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
We describe the methodology used to set up and compute spatial derivatives of the electron moments using data acquired by the Plasma Electron And Current Experiment (PEACE) from the four Cluster spacecraft. The results are used to investigate electron vorticity in the foreshock. We find that much of the measured vorticity, under nominal conditions, appears to be caused by changes in the flow direction of the return (either reflected or leakage from the magnetosheath) and strahl electron populations as they couple to changes in the magnetic field orientation. This in turn results in deflections in the total bulk velocity producing the measured vorticity.
- Published
- 2010
- Full Text
- View/download PDF
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