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Syngnathids are considered flagship species of estuarine and coastal environments. However, most of the Mediterranean species are still classified as data-deficient (DD) at a global level according to the IUCN Red List of Threatened Species. In central Italy, several areas host potentially suitable habitats for syngnathids but have not been previously reported in the literature; the estimation of population parameters and habitat partitioning at these sites may help to assess their conservation status. In this study, we investigated the abundance, distribution, and habitat preferences of sympatric populations of Hippocampus hippocampus, H. guttulatus, Syngnathus abaster, and Nerophis ophidion in Sabaudia Lake (Tyrrhenian Sea, Italy). While confirming the primary importance of a healthy coastal habitat, we retrieved hints about species ecology and habitat use. The species distribution in the study area highlights the role of habitat complexity in supporting local populations of these sensitive species. [ABSTRACT FROM AUTHOR]

Larnaca's salt lakes constitute one of the most important protected areas in Cyprus and in the southeast Mediterranean. It is in continuity with the urban area of Larnaca city, being the major green infrastructure in the area, supporting (among others) biodiversity, recreation, culture, and food production. The present study aims to provide an overview of the area's ecosystem types and their potential to provide ecosystem services, focusing on assessing the water ecosystem condition and drafting the relevant baseline accounts. The results of the study include an ecosystem type map of the area, followed by potential supply maps of ecosystem services, for the three major categories (i.e., provisioning, regulating and maintenance, and cultural) and the estimation and assessment of ecosystem condition variables for wetlands, as proposed by the EU-wide methodology to map and assess the ecosystem condition, in line with the System of Environmental Economic Accounting—Ecosystem Accounting (SEEA EA). A critical exceedance (over 10%) of the imperviousness and the increase in the soil moisture deficit within the wider salt lakes area have been identified and can constitute useful variables associated with the anthropogenic, climatic, and other effects on the condition of the salt lakes. Discussion for integrating this information to existing knowledge is provided toward supporting scientifically informed decision and policymaking for the sustainable development of protected areas. [ABSTRACT FROM AUTHOR]

Fronhofer et al. ([12])"s proposal undermines the usual 'slow evolution/fast ecology" wisdom that served for decades as a "good reason" for not considering ecology and evolution together. Indeed, let us imagine what the theories of ecology and evolution would have been if they had been written before the rise of multicellular organisms: disentangling ecology from evolution is difficult in the microbial realm if it even has any meaning. In this framework, ecology and evolution sensu stricto can be redefined by their relative speed with regard to evolution and ecology respectively (Figure 1). Keywords: biodiversity levels; concepts; deep-time eco-evolution; eco-evolutionary dynamics; environmental change; fast/slow processes; integrative models; overlooked variability; plasticity; tree of life EN biodiversity levels concepts deep-time eco-evolution eco-evolutionary dynamics environmental change fast/slow processes integrative models overlooked variability plasticity tree of life S5 S10 6 10/18/23 20230902 NES 230902 DEEPENING LINKS BETWEEN ECOLOGY AND EVOLUTION Developing deeper links between ecology and evolution is not a new idea and has been advocating since at least the 1950s (Futuyma, [13]), but most 20th-century attempts can be considered more as rapprochement than actual integration (Huneman, [17] for a detailed historical review). [Extracted from the article]

Grassland ecosystems undergo dramatic habitat loss and fragmentation worldwide due to agricultural expansion and intensification. Among different taxonomic groups, grassland arthropods are particularly sensitive to this process and, therefore, are exposed to considerable risk.Although many studies focus on land use impacts on arthropod diversity, these mostly consider taxonomic diversity and neglect trait‐mediated functional responses. However, studying functional diversity often provides more accurate insights into ecosystem processes.Here, we assessed the effect of fragment size and connectivity on the functional composition (community‐weighted mean trait values) and diversity (Rao's quadratic entropy) of two diverse and abundant arthropod groups: spiders and true bugs. We assigned functional traits for more than 300 species collected from 30 sandy steppe (forest‐steppes) and 30 loess steppe fragments (kurgans) in the southern part of the Hungarian Great Plain.Functional responses to fragmentation were observed for spiders in forest‐steppes and for true bugs in kurgans. We found higher functional diversity in better‐connected fragments for spiders and in small fragments for both groups. We collected small, humidity‐preferring and web‐building spiders in small fragments, and there were more ballooning spiders with moist habitat preferences in well‐connected fragments. Furthermore, increasing grassland connectivity resulted in a significantly higher proportion of polyphagous and humidity‐preferring individuals in the true bug communities.Our results highlight the importance of small grassland fragments in maintaining high arthropod functional diversity. Even small fragments in a well‐connected grassland network can promote ecosystem functioning and associated services within human‐dominated landscapes. [ABSTRACT FROM AUTHOR]

Simple Summary: Habitat loss profoundly influences animal behaviour, impacting the long-term viability of species. In this study, we explored the link between the flight capabilities of the woodland-specialist butterfly, Limenitis camilla, and the spatial-temporal connectivity of its main habitat—broadleaved forests. We examined the shifts in forest functional connectivity in Wallonia (Belgium) in recent decades, alongside the exploratory flight patterns of female L. camilla. Our findings revealed that butterflies from fragmented forests spent more time on movement-related behaviours than those from well-connected habitats. This indicates a potential evolutionary adaptation favouring behaviours that help locate suitable egg-laying sites in populations that have experienced habitat shrinkage and increased isolation over the past 20–40 generations. There is broad evidence that the main driver of the ongoing biodiversity crisis is land-use change, which reduces and fragments habitats. The consequence of habitat fragmentation on behavioural responses of fitness-related traits in insects have been so far understudied. In herbivorous insects, oviposition-related behaviours determine access to larval food, and the fate of the next generation. We present a pilot study to assess differences in behaviours related to movement and oviposition in Limenitis camilla butterflies from Wallonia (Belgium), one of the most fragmented regions in Europe. We first quantified variation in functional habitat connectivity across Wallonia and found that fragmented habitats had more abundant, but less evenly distributed host plants of L. camilla. Secondly, we quantified the behaviours of field-caught L. camilla females originating from habitats with contrasted landscape connectivity in an outdoor experimental setting. We found differences in behaviours related to flight investment: butterflies from fragmented woodlands spent more time in departing flight, which we associated with dispersal, than butterflies from homogenous woodlands. Although results from this study should be interpreted with caution given the limited sample size, they provide valuable insights for the advancement of behavioural research that aims to assess the effects of global changes on insects. [ABSTRACT FROM AUTHOR]

Simple Summary: Functional redundancy, the coexistence of species with similar functional roles, is of critical importance for ecosystem function stability. However, drivers of functional redundancy remain relatively poorly understood. Here, we analyzed four traits of British butterflies and aimed to identify the biotic and abiotic factors that affect the spatial patterns of functional redundancy. The most important factor was taxonomic diversity, with species-rich communities having the highest level of functional redundancy. Temperature was associated with redundancy and with different facets of taxonomic and functional diversity. However, although warmer areas hosted species-rich communities, redundancy was greatest in areas of intermediate mean annual temperature and declined at higher temperatures. This might imply that despite the positive effect of increased temperature on butterfly diversity, warmer and species-rich areas are vulnerable, perhaps due to the land uses dominating in these regions. Landscape heterogeneity promoted species richness, functional redundancy and variation in species and functional composition of the butterfly assemblages, underscoring the importance of maintaining diverse landscapes. Biodiversity promotes the functioning of ecosystems, and functional redundancy safeguards this functioning against environmental changes. However, what drives functional redundancy remains unclear. We analyzed taxonomic diversity, functional diversity (richness and β-diversity) and functional redundancy patterns of British butterflies. We explored the effect of temperature and landscape-related variables on richness and redundancy using generalized additive models, and on β-diversity using generalized dissimilarity models. The species richness-functional richness relationship was saturating, indicating functional redundancy in species-rich communities. Assemblages did not deviate from random expectations regarding functional richness. Temperature exerted a significant effect on all diversity aspects and on redundancy, with the latter relationship being unimodal. Landscape-related variables played a role in driving observed patterns. Although taxonomic and functional β-diversity were highly congruent, the model of taxonomic β-diversity explained more deviance than the model of functional β-diversity did. Species-rich butterfly assemblages exhibited functional redundancy. Climate- and landscape-related variables emerged as significant drivers of diversity and redundancy. Τaxonomic β-diversity was more strongly associated with the environmental gradient, while functional β-diversity was driven more strongly by stochasticity. Temperature promoted species richness and β-diversity, but warmer areas exhibited lower levels of functional redundancy. This might be related to the land uses prevailing in warmer areas (e.g., agricultural intensification). [ABSTRACT FROM AUTHOR]

An animal's ability to navigate its home range in search of essential resources is a key aspect of its ecology. To reach these resources, animals employ varying navigational processes depending on their exocentric or egocentric view of their environment. The goal of this study was to determine if the Javan slow loris (Nycticebus javanicus), a nocturnal arboreal primate found in southeast Asia, uses some form of cognitive map and spatial memory while navigating their environment. Using behavioural and GPS data of six males and seven females collected at the Little Fireface Project field station based in West Java, Indonesia, we measured their frequency of revisiting important feeding trees, route overlap, and points where individuals significantly changed directions. We found that all individuals predominantly used four tree species while feeding and foraging. The lorises also displayed a high level of route overlap, leading us to conclude that they likely utilize a route-based cognitive map where certain landmarks are integral to their nightly movement. Few studies have specifically focused on strepsirrhine spatial cognition in the wild; here, we show the navigational mechanism used by the Javan slow loris to reach distant/out-of-sight resources. The evident reliance on spatial cognition in a strepsirrhine species suggest that it could be an important selective pressure for primates at the earliest stages of primate cognitive evolution. In addition to the importance of spatial memory in theoretical discourse, understanding slow loris movement has practical applications to conservation, particularly regarding the numerous translocations undertaken by individuals rescued from the illegal wildlife trade. We discuss the importance of considering soft release training and monitoring in such releases. [ABSTRACT FROM AUTHOR]

Re-vegetation types and mycorrhizal fungi reclamation play a vital role in the improvement of soil quality in the mining subsidence of the northern Loess Plateau. However, the effects of re-vegetation types and mycorrhizal fungi reclamation on plant stoichiometric homeostasis, soil bacterial communities and functional characteristics are still not understood well but are vital for mining green construction. Based on the fact that mycorrhizal fungi reclamation has been implemented for more than 10 years (inoculation with arbuscular mycorrhizal fungi (AMF) and control), we examined five re-vegetation types with different C:N:P stoichiometry in the roots, leaves and calculated homeostasis. Meanwhile, second-generation sequencing technology was used to measure soil bacterial communities and functional characteristics to further reveal the relationships between soil factors and bacteria that drive plant stoichiometry and homeostasis in the biological reclamation area of coal mining subsidence. Our results indicated that plant N:P ratio in the leaves of all re-vegetation types was less than 14, with the highest ratio observed in A. fruticosa (nitrogen-fixing plants), showing that re-vegetation growth was limited by the availability of nitrogen. Only leaves in AMF-inoculated plants were categorized as 'homeostatic', while inoculation with AMF in both leaves and roots could alleviate nitrogen restriction and improve ecological stoichiometric homeostasis. The dominant phylum was Proteobacteria, followed by Actinobacteria, Acidobacteria, accounting for 69.92%–73.22% of all bacterial species and 82% with Chloroflexi. Soil copiotrophic community (Proteobacteria) in the AMF inoculation area was higher than those in the control area under all re-vegetation types, while the oligotrophic community (Acidobacteria) was lower than the control. Further analysis showed that soil TP, SOC, C:N and HD played vital roles in shifting the soil bacteria community. Soil stoichiometry and AMF affect microbial composition. These results indicated that the re-vegetation types and mycorrhizal fungi reclamation could shift bacterial homogeneity. Hence, our results expound that mycorrhizal fungi reclamation could optimize the ecological strategies of reclaimed vegetation, alleviate N-limitations in plants, improve endogenous stability and promote the ecological function of soil bacteria, which provided theoretical bases for further understanding and application of green restoration and sustainable development in the mining subsidence of the northern Loess Plateau. [ABSTRACT FROM AUTHOR]

Simple Summary: The Himalayan marmot is a host animal for plague-causing pathogens and is endemic to the Qinghai–Tibet Plateau (QTP). With human activities and global warming, the Himalayan marmot population has increased rapidly, causing damage to grassland ecosystems and increasing the risk of plague infection in humans. The current and future potential distribution of the Himalayan marmot has significant implications for ecosystem management, biodiversity conservation, and public health safety on the QTP. The maximum entropy model was employed to analyze the Himalayan marmot potential distribution in the near and later future (2050s and 2070s) in this study. Under future climate scenarios, the uninterrupted rise in precipitation and temperature over the QTP predicted that the suitable area for the Himalayan marmot will increase, with the centroid shifting to higher latitudes. Our results indicate that climatic factors are of great significance for the distribution of the Himalayan marmot and provide a reference for ecosystem management and the monitoring of plague epidemics in the QTP. Climate warming and human activities impact the expansion and contraction of species distribution. The Himalayan marmot (Marmota himalayana) is a unique mammal and an ecosystem engineer in the Qinghai–Tibet Plateau (QTP). This pest aggravates grassland degradation and is a carrier and transmitter of plagues. Therefore, exploring the future distribution of Himalayan marmots based on climate change and human activities is crucial for ecosystem management, biodiversity conservation, and public health safety. Here, a maximum entropy model was explored to forecast changes in the distribution and centroid migration of the Himalayan marmot in the 2050s and 2070s. The results implied that the human footprint index (72.80%) and altitude (16.40%) were the crucial environmental factors affecting the potential distribution of Himalayan marmots, with moderately covered grassland being the preferred habitat of the Himalayan marmot. Over the next 30–50 years, the area of suitable habitat for the Himalayan marmot will increase slightly and the distribution center will shift towards higher latitudes in the northeastern part of the plateau. These results demonstrate the influence of climate change on Himalayan marmots and provide a theoretical reference for ecological management and plague monitoring. [ABSTRACT FROM AUTHOR]

Harvester ants are one of the most extensively studied groups of ants, especially the group foraging ants, Messor barbarus (Linnaeus, 1767), which construct long-lasting trunk trails. Limited laboratory investigations have delved into head-on encounters along foraging trails involving workers moving in opposing directions, with fewer corresponding studies conducted in the natural environment. To address this gap, we devised an in-field experimental design to induce lane segregation on the foraging trunk trail of M. barbarus. Using an image-based tracking method, we analyzed the foraging behavior of this species to assess the costs associated with head-on encounters and to figure out the natural coexistence of outgoing and returning workers on a bidirectional route. Our results consistently reveal heightened straightness and speed in unidirectional test lanes, accompanied by an elevated foraging rate compared to bidirectional lanes. This suggests a potential impact of head-on collisions on foraging behavior, especially on foraging efficiency. Additionally, Kinematic analysis revealed distinct movement patterns between outbound and inbound flows, particularly low speed and sinuous trajectories of inbounding unladen workers. The study of encounter rates in two traffic systems hints at the plausible utilization of individual memory by workers within trails, underscoring the pivotal role of encounters in information exchange and load transfer., (© 2024 Institute of Zoology, Chinese Academy of Sciences.)

Climate change threatens many species by a poleward/upward movement of their thermal niche. While we know that faster movement has stronger impacts, little is known on how fluctuations of niche movement affect population outcomes. Environmental fluctuations often affect populations negatively, but theory and experiments have revealed some positive effects. We study how fluctuations around the average speed of the niche impact a species' persistence, abundance and realized niche width under climate change. We find that the outcome depends on how fluctuations manifest and what the relative time scale of population growth and climate fluctuations are. When populations are close to extinction with the average speed, fluctuations around this average accelerate population decline. However, populations not yet close to extinction can increase in abundance and/or realized niche width from such fluctuations. Long-lived species increase more when their niche size remains constant, short-lived species increase more when their niche size varies., (© 2024 The Author(s). Ecology Letters published by John Wiley & Sons Ltd.)

The nutritional diversity of resources can affect the adaptive evolution of consumer metabolism and consumer diversification. The omega-3 long-chain polyunsaturated fatty acids eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) have a high potential to affect consumer fitness, through their widespread effects on reproduction, growth and survival. However, few studies consider the evolution of fatty acid metabolism within an ecological context. In this review, we first document the extensive diversity in both primary producer and consumer fatty acid distributions amongst major ecosystems, between habitats and amongst species within habitats. We highlight some of the key nutritional contrasts that can shape behavioural and/or metabolic adaptation in consumers, discussing how consumers can evolve in response to the spatial, seasonal and community-level variation of resource quality. We propose a hierarchical trait-based approach for studying the evolution of consumers' metabolic networks and review the evolutionary genetic mechanisms underpinning consumer adaptation to EPA and DHA distributions. In doing so, we consider how the metabolic traits of consumers are hierarchically structured, from cell membrane function to maternal investment, and have strongly environment-dependent expression. Finally, we conclude with an outlook on how studying the metabolic adaptation of consumers within the context of nutritional landscapes can open up new opportunities for understanding evolutionary diversification., (© 2021 The Authors. Ecology Letters published by John Wiley & Sons Ltd.)

Almost 50 years ago, Michael Rosenzweig pointed out that nutrient addition can destabilise food webs, leading to loss of species and reduced ecosystem function through the paradox of enrichment. Around the same time, David Tilman demonstrated that increased nutrient loading would also be expected to cause competitive exclusion leading to deleterious changes in food web diversity. While both concepts have greatly illuminated general diversity-stability theory, we currently lack a coherent framework to predict how nutrients influence food web stability across a landscape. This is a vitally important gap in our understanding, given mounting evidence of serious ecological disruption arising from anthropogenic displacement of resources and organisms. Here, we combine contemporary theory on food webs and meta-ecosystems to show that nutrient additions are indeed expected to drive loss in stability and function in human-impacted regions. Our models suggest that destabilisation is more likely to be caused by the complete loss of an equilibrium due to edible plant species being competitively excluded. In highly modified landscapes, spatial nutrient transport theory suggests that such instabilities can be amplified over vast distances from the sites of nutrient addition. Consistent with this theoretical synthesis, the empirical frequency of these distant propagating ecosystem imbalances appears to be growing. This synthesis of theory and empirical data suggests that human modification of the Earth is strongly connecting distantly separated ecosystems, causing rapid, expansive and costly nutrient-driven instabilities over vast areas of the planet. Similar to existing food web theory, the corollary to this spatial nutrient theory is that slowing down spatial nutrient pathways can be a potent means of stabilising degraded ecosystems., (© 2020 John Wiley & Sons Ltd.)

Due to the problems of relatively fragile stability, the quality of soil in the drip-irrigated agricultural ecosystem has high spatial heterogeneity and experiences significant degradation. We conducted a two-year field plot study (2021–2022) in a typical region of the arid zone with the "wolfberry" crop as the research object, with three irrigation and three nitrogen application levels, and the local conventional management as the control (CK). Soil quality under experimental conditioning was comprehensively evaluated based on Principal Component Analysis (PCA) and Technique for Order Preference by Similarity to an Ideal Solution (TOPSIS), and regression analyses were carried out between the soil quality evaluation results and wolfberry yield. The results showed that short-term water and nitrogen regulation enhanced the soil nutrient content in the root zone of wolfberry to some extent, but it did not significantly affect soil carbon:soil nitrogen (Csoil:Nsoil), soil carbon:soil phosphorus (Csoil:Psoil), and soil nitrogen:soil phosphorus (Nsoil:Psoil). When the irrigation quota was increased from I1 to I2, the soil microbial biomass carbon, nitrogen, and phosphorus (Cmic, Nmic, and Pmic) tended to increase with the increase in N application, but the microbial biomass carbon:nitrogen (Cmic:Nmic), microbial biomass carbon:phosphorus (Cmic:Pmic), and microbial biomass nitrogen:phosphorus (Nmic:Pmic) did not change significantly. The comprehensive evaluation of the principal components and TOPSIS showed that the combined soil nutrient–microbial biomass and its ecological stoichiometry characteristics were better under the coupled treatments of I2, I3, N2, and N3, and the overall soil quality under these treatment conditions was significantly better than that under the CK treatment. Under I1 irrigation, nitrogen application significantly increased the yield of wolfberry, while under I2 and I3 irrigation, the wolfberry yield showed a parabolic trend with the increase in nitrogen application. The highest yield was recorded in the I2N2 treatment in the first and second years, with yields of 9967 kg hm−2 and 10,604 kg hm−2, respectively. The coefficient of determination (explained quantity) of the soil quality based on soil nutrient–microbial biomass and the characteristics of its ecological stoichiometry for wolfberry yield ranged from 0.295 to 0.573. These findings indicated a limited positive effect of these indicators of soil on wolfberry yield. The short-term water and nitrogen regulation partly influenced the soil and soil microbial biomass in agroecosystems, but the effect on elemental balance was not significant. Our findings might provide theoretical support for managing the health of agricultural ecosystems. [ABSTRACT FROM AUTHOR]

The composition of urban forests (UFs) exhibits homogenization among cities compared with rural forests (RFs) among different climate zones. However, the degree of homogenization in subtropical UFs and its difference from RFs remain unclear. In this study, we examined tree species composition and diversity in UFs in 19 cities in China's subtropical zone with precipitation ranging from 458 to 1852 mm and compared them with RFs. We found that (1) the species composition similarity, Jaccard index (J~0.27), between UFs was significantly higher than that (J~0.15) of RFs, indicating biotic homogenization; (2) tree species richness, Simpson, Shannon–Wiener, and Pielou index of UFs converged along the precipitation gradient; (3) the similarity of tree composition between UFs increased as precipitation of the cities were more similar; (4) the UFs in the 19 cities contained a total of 932 tree species, among which the nonnative species were more prevalent than the native species, and the top 37 species with high frequency appeared in 80% of the cities; and (5) Salix babylonica, Ginkgo biloba, Platycladus orientalis, Juniperus chinensis, and other tree species were suitable for planting in UFs in subtropical zones, regardless of humidity. The findings contribute to the understanding of urban forest development and provide insights for implementing greening policies aimed at providing additional ecosystem services. [ABSTRACT FROM AUTHOR]

Over the course of the last 20 years, numerous studies have identified the benefits of thermal waters on different skin conditions. Consequently, several investigations have been carried out on their effects on the skin, which are linked to their chemical composition, and, recently, scientists have turned their attention to the role of the thermal spring's microbiota, named "hydrobiome", regarding these therapeutic effects. At the same time, the development of cosmetics based on pre, pro, and postbiotics has reached great relevance and research is increasing every day. This review gathers information on the biological diversity of thermal spring waters and their potential use in obtaining biological compounds, metabolites, or bacterial extracts for use in dermocosmetics as active ingredients. These bioactive compounds are able to improve dermatological diseases such as atopic dermatitis or rosacea and ameliorate pruritus and xerosis; moreover, they can increase protection against UV exposure, strengthen barrier function, maintain good homeostasis of skin defenses, repair damaged skin, promote wound healing, improve skin condition, reduce uneven skin pigmentation, and prevent skin aging. From a future perspective, fruitful cooperation among researchers, hydrologists, thermal spa centers, and cosmetic industries will drive this sector toward a better understanding of the role of the hydrobiome of thermal spring waters on healthy skin and dermatological diseases and consider the inclusion of derivatives of this hydrobiome (in the form of fermenters, lysates, extracts, etc.) in dermocosmetic formulations. Therefore, and being aware of the potential of the hydrobiome in dermatological and skin care applications, the future prospects for the use of bioactive substances derived from it in dermocosmetic formulations are promising. [ABSTRACT FROM AUTHOR]

Mosquito-borne La Crosse virus (LACV; family: Peribunyaviridae) is the leading cause of pediatric arboviral encephalitis in the United States, with clinical cases generally centered in the Midwest and Appalachian regions. Incidence of LACV cases in Appalachian states has increased, such that the region currently represents the majority of reported LACV cases in the USA. The amount of reported LACV cases from Virginia, however, is minimal compared to neighboring states such as North Carolina, West Virginia, and Tennessee, and non-Appalachian regions of Virginia are understudied. Here we examine the hypothesis that LACV is circulating widely in Virginia, despite a low clinical case report rate, and that the virus is circulating in areas not associated with LACV disease. In this study, we screened local mammalian wildlife in northwestern counties of Virginia using passive surveillance via patients submitted to wildlife rehabilitation centers. Blood sera (527 samples; 9 species, 8 genera) collected between October 2019 and December 2022 were screened for neutralizing antibodies against LACV, indicating prior exposure to the virus. We found an overall LACV seroprevalence of 1.90% among all wild mammals examined and reveal evidence of LACV exposure in several wild species not generally associated with LACV, including eastern cottontails and red foxes, along with established reservoirs, eastern gray squirrels, although there was no serological evidence in chipmunks. These data indicate the circulation of LACV in Virginia outside of Appalachian counties, however, at a lower rate than reported for endemic areas within the state and in other states. [ABSTRACT FROM AUTHOR]

C, N and P are the key biogenic elements of terrestrial ecosystems, and their biogeochemical processes regulate nutrient cycling and play a key role in restoring degraded ecosystems. In this paper, the grassland ecosystem under artificial restoration measures (Dactylis glomerata (DG), Lolium perenne (LP), Lolium perenne + Trifolium repens (LT)), and the natural restoration measures (NG) in a typical karst plateau desertification control area of southwest China, were taken as the research object. The C, N, and P concentrations and the ecological stoichiometry of the plant–soil–microorganism system in grasslands under different restoration measures in the karst desertification area were explored. We established the following findings: (1) Compared with NG, the C, N and P concentrations of plants and soil in DG, LP and LT were higher, and LT was the highest. (2) The microbial biomass carbon (MBC), microbial biomass nitrogen (MBN) and microbial biomass phosphorus (MBP) concentrations in LT was also the highest. (3) The C:N ratio of plant and soil indicated that the N mineralization and nutrient release rate of DG, LP and LT were stronger than that of NG, and the plant growth of NG was most seriously limited by nitrogen. The N:P ratio in plant and soil indicated that the grassland was limited by P in the four treatments. (4) The result of correlation analysis showed that the cycling process of C, N and P in the plant–soil–microorganism system was coupled, and that the elements closely affected each other. In general, the effect of artificial restoration on a degraded ecosystem was relatively better than the natural restoration for increasing plant, soil and microbial nutrient concentrations, especially in the mixed-seed ecosystem of leguminous and gramineous forage. However, in the future, grassland management, appropriate N fertilizer or N-fixing plants and P fertilizer should be considered to improve the limitation of plant N and P, so as to realize the sustainable development of grasslands and the restoration of degraded ecosystems in the karst desertification control area. [ABSTRACT FROM AUTHOR]

Information on the movement ecology of species can assist with identifying barriers to dispersal and appropriate management actions. We focus on the threatened Malleefowl (Leipoa ocellata) whose ability to move and disperse within fragmented landscapes is critical for their survival. We also investigate the possible effects of climate change on Malleefowl movement. We used solar‐powered GPS telemetry to collect movement data and determine the influence of breeding status, remnant vegetation patches and environmental variables. Seven Malleefowl were tracked between 1 and 50 months, resulting in 20 932 fixes. While breeding, Malleefowl had significantly smaller home ranges (92 ± 43 ha breeding; 609 ± 708 ha non‐breeding), moved shorter daily distances (1283 ± 605 breeding; 1567 ± 841 non‐breeding) and stayed closer to the incubation mound (349 ± 324 m breeding; 3293 ± 2715 m non‐breeding). Most Malleefowl effectively disassociated from the mound once breeding stopped, with two birds dispersing up to 10.2 km. Movement patterns were significantly correlated with the size of the remnant native vegetation patch, with smaller home ranges being utilized in small patches than in large patches. One male almost exclusively remained within a 107‐ha patch for over 4 years, but a female crossed between closely spaced uncleared patches. Long‐range movements of nearly 10 km daily displacement were recorded in large remnants almost exclusively when not breeding. Temperature and rain had a significant effect on movement: modelling suggests daily distances decline from 1.3 km at 25°C to 0.9 km at 45°C, with steeper declines over 30°C. The influence of patch size on movement patterns suggests that Malleefowl movement may be governed by the size of remnant patches and that habitat continuity may be important for facilitating recolonization after catastrophic events and maintaining genetic diversity. Climate change may reduce Malleefowl movement during hot, dry periods possibly affecting breeding success. [ABSTRACT FROM AUTHOR]

The responses of dominant species to plant–soil feedback (PSF) are well established; however, the changes in the PSF of companion species remain unclear. This study aims to assess the direction and intensity of PSF, determine the main factors influencing it, and interpret the ecological significance of PSF in companion species within the context of grassland community succession. Three typical companion species, namely Artemisia sacrorum, Artemisia capillaris, and Artemisia giraldii, were planted in soils at three grassland community succession stages (early, middle, and late) on the Loess Plateau. Our results indicate that during both plant growth periods, the shoot biomass of A. sacrorum, A. capillaris, and A. giraldii in early- and late-stage soils was higher than that in the middle-stage soil, suggesting consistent growth of the three companion species during the two growth periods. However, plant growth simultaneously led to a reduction in soil nutrient content and microbial biomass, resulting in an overall decrease in the biomass of the three species, indicating a negative PSF effect in companion species. In conclusion, the negative PSF observed in all three associated species explains the temporary dominance of companion species during succession. This study enhances our understanding of the mechanisms driving PSF in community succession. [ABSTRACT FROM AUTHOR]

Vegetation, as a comprehensive ecological indicator of environmental change, plays an essential role in terrestrial ecosystems. Dramatic climate change and increasing anthropogenic disturbances have led to a profound change in vegetation cover. This study employed Moderate Resolution Imaging Spectroradiometer (MODIS) and other relevant data in Southwest China mountains, using Chongqing as a case study, to explore the variation in Normalized Difference Vegetation Index (NDVI) and its spatial differentiation mechanisms, quantify the relative contributions of climate change and human activities to these variations, and assess the effects of ecological policies, including afforestation, mountain closure forestry and natural forest conservation, and agricultural improvement. Results showed that the spatial distribution of annual NDVI, on average, varied 0.109 to 0.929, with the rate varying from −0.033 to 0.017 yr−1. NDVI was improved in 83.4% areas of Chongqing, and human activities contributed 69.1% of revegetation, whereas climate contributed 30.9%. On the other hand, human activities and climate change contributed 76.3% and 23.7% to vegetation degradation in 17.6% areas, respectively. Moreover, the explanatory power of anthropogenic factors on spatial differentiation of NDVI were stronger than that of climatic factors, and land use type was the dominant factor. The cumulative afforestation area, effective irrigation rate, fertilizer application, and total agricultural machinery power of the whole city was extremely significantly correlated with NDVI (p < 0.01), and the vegetation cover fluctuation in the region affected by natural forest protection and mountain closure forestry project was much more stable than that in non-protected areas, which to a certain extent reflected the effective implementation of local ecological protection, agricultural improvement, and other related policies. [ABSTRACT FROM AUTHOR]

Soil organic carbon (SOC) fractions are influenced by inputs of nitrogen (N) from globally rising N deposition; however, the mechanisms of how soil microbiological properties are influenced by N deposition and its impact on the fractional distribution and stability of SOC remain unclear. In this study, we assessed the effects on SOC fraction distribution and stability from four aspects of soil microbiological properties: soil microbial biomass (SMB), soil microbial activity, structure diversity, and functional diversity of soil microbial community in a Pinus tabuliformis plantation, which received four N addition levels (0 g N m−2 y−1 (N0), 3 g N m−2 y−1 (N3, low N addition), 6 g N m−2 y−1 (N6, mid-N addition), and 9 g N m−2 y−1 (N9, high N addition)) for 2 years. The N inputs did significantly affect some soil microbiological properties, like SMB, soil phospholipid fatty acid (PLFA), and soil microbial functional diversity. Mid- and high N addition decreased the richness (HPLFA) and evenness (EPLFA) index of the soil microbial community, from 3.24 to 2.91 and 0.93 to 0.87, respectively. In addition, the low N addition promoted the carbon management index (CMI) to 141.35, i.e., higher than the CMIs in the mid- and high-level treatments. The SOC stability also showed significant differences among N addition treatments, and SOC could be the most stable at the mid-N addition level. Regarding the effects of the four soil microbiological attributes on the CMI and stability, SMB and soil respiration positively impacted the CMI, but did not significantly affect the stability. In addition, EPLFA had positive effects, but EBIOLOG had negative effects on CMI and lability. Our findings indicate that soil microbiological properties are essential in SOC fractional distributions and stability. Further identification and study of soil microbial species used to change SOC fractions would help to clarify the detailed mechanisms involved. [ABSTRACT FROM AUTHOR]

Green space is essential in urban areas to maintain, and improve human well-being. To better understand the impact of environmental and socioeconomic changes on the sustainability of tropical urban green space landscapes, work is needed to explore the patterns of plant diversity and its drivers in urban green spaces. We explore urban floristic diversity patterns based on samples from 826 urban functional units located in five cities in the tropical coastal region of China. Field surveys were conducted to obtain data on plant diversity, land use types, socioeconomic characteristics, and environmental characteristics within these units. Plant diversity within the cities varied significantly among land-use types, with high-rise housing, parks, and universities exhibiting higher diversity. The diversity of cultivated plant species and the regional economy showed a significant positive correlation (β coefficient = 0.15, p-value < 0.05), while an increase in the diversity of spontaneously growing plant species and native species was linked to building age (β coefficient = 0.12, p-value < 0.01; β coefficient = 0.13, p-value < 0.01). Management also affected plant species diversity, with watering and maintenance frequency having a positive association. Urban plant diversity patterns result from a combination of multiple factors, and different drivers affect native vs. non-native plant diversity Socio-economic factors are the primary driver of urban plant diversity patterns, as space management and maintenance determine what can grow in different areas. This study has important practical significance for policymakers and managers in developing and managing urban green spaces more sustainably. [ABSTRACT FROM AUTHOR]

The monkeypox virus causes a respiratory illness called monkeypox, which belongs to the Poxviridae virus family and the Orthopoxvirus genus. Although initially endemic in Africa, it has recently become a global threat with cases worldwide. Using the Antangana–Baleanu fractional order approach, this study aims to propose a new monkeypox transmission model that represents the interaction between the infected human and rodent populations. An iterative method and the fixed-point theorem are used to prove the existence and uniqueness of the symmetry model's system of solutions. It shows that the symmetry model has equilibrium points when there are epidemics and no diseases. As well as the local asymptotic stability of the disease-free equilibrium point, conditions for the endemic equilibrium point's existence have also been demonstrated. For this purpose, the existence of optimal control is first ensured. The aim of the proposed optimal control problem is to minimize both the treatment and prevention costs, and the number of infected individuals. Optimal conditions are acquired Pontryagin's maximum principle is used. Then, the stability of the symmetry model is discussed at monkeypox-free and endemic equilibrium points with treatment strategies to control the spread of the disease. Numerical simulations clearly show how necessary and successful the proposed combined control strategy is in preventing the disease from becoming epidemic. [ABSTRACT FROM AUTHOR]

Despite the global decline in marine species biodiversity, relatively few countries have enacted national endangered and threatened species legislation. Tailoring an adequate legislative framework with clear objectives and regulations consistent with the available scientific evidence is fundamental for the effective conservation of marine endangered and threatened species. This paper analyzes the legal framework and current institutional tools for the conservation of marine endangered and threatened species in the Russian Federation. In this regard, important legislative tools include federal laws, as well as internationally binding signed agreements, among others, such as the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and the Convention on Wetlands of International Importance Especially as Waterfowl Habitat (Ramsar). A strategy and an action plan for the conservation of biological diversity were also developed. Besides, the most important tool for the conservation and protection of marine endangered and threatened species is the Red Book of the Russian Federation (RBRF) and other regional Red Books. Responsibility for causing harm to the species listed in the RBRF and their habitat is specified in the code of administrative offenses and the criminal code of the Russian Federation. Finally, conclusions and identified gaps were highlighted in the last section, including, among other things, that legislation is still limited in how it takes the impacts of climate change into account. Such type of study is highly recommended, considering the relatively few number of papers dedicated to the study of the impact and/or implications of the conservation tools and strategies mentioned in this paper on the status of the marine endangered and threatened species. [ABSTRACT FROM AUTHOR]

Effective conservation of stream fishes and their habitats is complicated by the fact that human stressors alter the way in which natural factors such as stream size, catchment geology, and regional climate influence stream ecosystems. Consequently, efforts to assess the condition of stream fishes and their habitats must not only attempt to characterize the effects of human stressors but must account for the effects of natural influences as well. This study is an assessment of all stream fish habitats in the Mississippi River basin, USA. The basin supports over 400 stream fish species, drains a land area of 3.2 M km2, and includes a myriad of human stressors such as intensive agriculture, urbanization, nutrient loading, and habitat fragmentation by dams and road/stream crossings. To effectively characterize types and levels of human stressors specifically impacting the basin's stream fish species, our assessment approach first accounted for the influence of natural landscape conditions on species abundances with multiple steps, including stratifying our analyses by region and stream size and quantitatively modeling the influences of natural factors on stream fishes. We next quantified individual fish species responses to explicit human stressors for different measures of land use, fragmentation, and water quality, including summaries of measures in local vs. catchment extents. Results showed that many species had negative threshold responses to human stressors and that impacts varied by species, by region, and by the spatial extents in which stressors were summarized. Our spatially explicit results indicated the degree of stream reach impairment for specific stressor categories, for individual species, and for entire assemblages, all of which are types of information that can aid decision makers in achieving specific conservation goals in the region. [ABSTRACT FROM AUTHOR]

Urban areas are rapidly expanding into natural habitats worldwide. When species are threatened with habitat loss, it is vital to understand how they will respond or adapt to the change in their environment. One primate species threatened by habitat loss is the fully arboreal Javan slow loris (Nycticebus javanicus). This non-leaping species not only relies on canopy continuity but is also subject to capture for illegal wildlife trade, especially in anthropogenic landscapes where they are easier to catch. We examine the use of urban areas by Javan slow lorises in terms of habitat use as well as feeding and terrestriality behaviours in the municipality of Cipaganti in West Java, Indonesia. For this study, we observed Javan slow lorises from May 2018 to April 2020 for two dry periods and two wet periods. We combined home ranges and core areas with a land cover classification of the area to understand the composition of the loris habitat. We also included feeding and terrestriality data to determine the ratio of these activities within each land cover class. We found that approximately half of their territory falls into natural areas (bamboo patches: 45–60%), and the other half is in human land use areas (agriculture, shade gardens, urban areas, and fallow land). Urban areas ranged from 0 to 54% of an individual loris's habitat, with the proportion of urban land cover being higher in some individuals' core areas than in their home ranges. Only urban areas showed a variation between periods, with p-values of 0.06 and 0.002 for home ranges and core areas, respectively, showing a significant increase usage during dry periods. Of all feeding observations, 4% occurred in urban areas with nectar being the most common feeding item. We recorded thirteen different food species in urban areas with Calliandra calothrysus being the most frequent. We found that 7% of terrestriality events occurred in urban areas. The findings from this study show that human land use areas cover a significant portion of the Javan slow lorises habitat in this region, further emphasising the need to consider the needs of these Critically Endangered primates when developing natural habitats into those of human managed landscapes. The same principles are true for the large number of species that are able to persist in urban areas in a world increasingly dominated by humans. [ABSTRACT FROM AUTHOR]

C, N and P play an important indicator role in explaining the material cycles and elemental balances of living and non-living systems. In order to control karst rocky desertification, China has established a large number of artificial grasslands for the development of herbivorous animal husbandry, which has played an important role in ecological restoration and economic development. However, the effects of different use patterns on the ecological stoichiometry of the carbon (C), nitrogen (N) and phosphorus (P) of the grassland plant–soil–microorganism are not clear. In this study, the effects of grazing grassland (GG), mowing grassland (MG) and enclosed grassland (EG) on C, N and P and their ecological stoichiometry in the artificial grassland plant–soil–microbe were investigated in the karst desertification control area in Southern China. The results showed that (1) the C content was EG > GG > MG. The N content was GG > EG > MG, while the P content was MG > GG > EG. C:N, C:P and N:P were shown as EG > GG > MG. The plant N:P was more than 20, indicating a P deficiency and limitation, especially in EG. (2) The content of C and P in soil was EG > GG > MG. The N content was GG > EG > MG. The soil C:N showed EG > MG > GG, while C:P and N:P were shown as MG > GG > EG. The soil N:P ratios were all less than 14, indicating that all of them had an obvious N limitation. (3) Soil microbial biomass carbon (MBC) was GG > MG > EG. Soil microbial biomass nitrogen (MBN) was GG > EG > MG. Soil microbial biomass phosphorus (MBP) showed EG > GG > MG. MBC:MBN was MG > EG > GG. MBC:MBP was MG > EG > GG. MBN:MBP was GG > MG > EG. The MBN:MBP in GG and MG was greater than 9.6, which is P-limited, while the MBN:MBP in EG is less than 8.9, which is N-limited. (4) Plant C and N were significantly correlated with soil C and N, but plant P was significantly negatively correlated with soil P, while MBP was significantly positively correlated with soil TP. Soil microorganisms had the tendency to assimilate available P in GG and MG treatments, but the potential of releasing P from mineralized soil organic matter was higher in EG treatment. The results showed that the chemical properties and stoichiometric characteristics of the plant–soil–microorganism were significantly changed by different grassland-use methods, which provided scientific guidance for the management of C, N and P elements and the further optimization of soil microbial environment for artificial grassland in the karst rocky desertification area. [ABSTRACT FROM AUTHOR]

On a planet experiencing constant human population growth, it is necessary to explore the anthropogenic effects on the genetic diversity of species, and specifically invasive species. Using an analysis that integrates comparative phylogeography, urban landscape genetics, macrogenetics and a systematic review, we explore the worldwide genetic diversity of the human commensal and anthropogenic species Rattus rattus and Rattus norvegicus. Based on metadata obtained considering 35 selected studies related to observed heterozygosity, measured by nuclear molecular markers (microsatellites, Single Nucleotide Polymorphisms—SNPs-, restrictition site-associated DNA sequencing -RAD-Seq-), socioeconomic and mobility anthropogenic factors were used as predictors of genetic diversity of R. rattus and R. norvegicus, using the Gini index, principal component analysis and Random Forest Regression as analysis methodology. Population density was on average the best predictor of genetic diversity in the Rattus species analyzed, indicating that the species respond in a particular way to the characteristics present in urban environments because of a combination of life history characteristics and human-mediated migration and colonization processes. To create better management and control strategies for these rodents and their associated diseases, it is necessary to fill the existing information gap in urban landscape genetics studies with more metadata repositories, with emphasis on tropical and subtropical regions of the world. [ABSTRACT FROM AUTHOR]

Despite the important ecological role of forest‐steppes in nature conservation, information on the contribution of individual components to arthropod conservation is scarce. Furthermore, the effect of landscape structure on the arthropod composition of a natural mosaic of habitats has been largely understudied.We investigated the effects of habitat type and landscape heterogeneity on spider diversity in forest‐steppes of Kiskunság National Park, Hungary. We sampled ground‐dwelling spiders using pitfall traps in the grasslands, forest edges and forest interiors along a gradient of landscape composition (forest amount) and configuration (edge length) within 18 landscapes.We collected a total of 22,550 adult specimens belonging to 153 species. The three habitats of forest‐steppes showed a distinct community composition with a characteristic set of species traits. Xerothermic species of web‐building spiders were primarily found in grasslands, whereas spiders with a preference for moisture habitats were in the forest interior. The forest edges had higher species richness than forest interiors. The trait diversity, measured as RaoQ, was the highest in grasslands, while the lowest in the forests. We found that the increasing amount of forests in the landscape positively affected species richness in grasslands but negatively in forest edges and interiors. Edge length did not have any effect on spider communities.Increased species richness in grasslands with the increasing amount of forests highlights that grasslands are more affected by spillover from the neighbouring forests than vice versa. Habitat type plays a more important role in shaping spider diversity of natural forest‐steppe patches than landscape structure. However, landscape structure could modify the effect of habitat type. It emphasises the importance of habitat complementarity and therefore the high value of all components of the forest‐steppe landscape. [ABSTRACT FROM AUTHOR]

Simple Summary: We investigated the vertical transmission potential of the Orthobunyavirus La Crosse virus (LACV) in the mosquitoes Ae. albopictus and Ae. aegypti. In both mosquito species, productive midgut infection with the virus was a prerequisite for the following ovary infection. However, the midgut infection levels varied between both species, with Ae. albopictus being more susceptible to LACV infection than Ae. aegypti. In both mosquito species, the vertical transmission rates of LACV from mothers to offspring were below those levels typically described for transovarial transmission (TOT). The LACV infection patterns in the ovarian tissue of Ae. aegypti suggested the transovum transmission of the virus. In several Ae. albopictus samples, LACV antigen was detected in follicular tissue or in a few developing oocytes, indicating that the TOT of LACV could be potentially occurring in this mosquito species. Thus, TOT is not a general feature of LACV infections in mosquitoes. La Crosse virus (LACV) is circulating in the midwestern and southeastern states of the United States and can cause human encephalitis. The main vector of the virus is the eastern tree-hole mosquito, Aedes triseriatus. Ae. albopictus has been also described as a natural LACV vector, while Ae. aegypti has been infected with the virus under laboratory conditions. Here, we compare the vertical transmission potential of LACV in Ae. albopictus and Ae. aegypti, with emphasis given to the ovarian infection patterns that the virus generates in both species. Both mosquito species received artificial bloodmeals containing LACV. At defined time points post-infection/bloodmeal, midguts, head tissue, and ovaries were analyzed for the presence of virus. Viral infection patterns in the ovaries were visualized via immunofluorescence confocal microscopy and immunohistopathology assays using an LACV-specific monoclonal antibody. In Ae. aegypti, LACV was confronted with midgut infection and escape barriers, which were much less pronounced in Ae. albopictus, resulting in a significantly higher prevalence of infection in the latter. Following the ingestion of a single virus-containing bloodmeal, no progeny larvae were found to be virus-infected. Regardless, females of both species showed the presence of LACV antigen in their ovariole sheaths. Furthermore, in a single Ae. albopictus female, viral antigen was associated with the nurse cells inside the primary follicles. Following the ingestion of a second non-infectious bloodmeal at 7- or 10-days post-ingestion of an LACV-containing bloodmeal, more progeny larvae of Ae. albopictus than of Ae. aegypti were virus-infected. LACV antigen was detected in the egg chambers and ovariole sheaths of both mosquito species. Traces of viral antigen were also detected in a few oocytes from Ae. albopictus. The low level of vertical transmission and the majority of the ovarian infection patterns suggested the transovum rather than transovarial transmission (TOT) of the virus in both vector species. However, based on the detection of LACV antigen in follicular tissue and oocytes, there was the potential for TOT among several Ae. albopictus females. Thus, TOT is not a general feature of LACV infection in mosquitoes. Instead, the TOT of LACV seems to be dependent on its particular interaction with the reproductive tissues of a female. [ABSTRACT FROM AUTHOR]

Purpose: Online resources can be helpful for students and can augment the content presented in learning environments. A team consisting of four biologists, a graduate student, instructional designer and media developers collaborated on the design, development and evaluation of first-year biology online tutorials in a Canadian University. The tutorials were designed to address knowledge gaps resulting in low success rates and attrition of first-year students in biology. The decrease in the number of students in STEM has alarmed educators, prompting a call for efforts to increase STEM majors in universities. Large class sizes, such as first year biology with ∼900 registrants annually, with detail-oriented, content-heavy loads, can result in low success rates and attrition. Design/methodology/approach: Active learning methods, including online formative assessments, which encourage student engagement in course material, can be effective in large introductory science classes, and thus, the authors provided engagement with tutorial online resources. The authors identified the tutorial topics by analyzing previous years' tests, student feedback and pedagogical research in undergraduate biology. The top five topics identified as common misconceptions or troublesome concepts within the course were selected. Standard instructional design processes were used to produce high-quality online tutorials. Tutorials included learning materials, videos, animations, self-assessments, reflective questions and badges to facilitate deep learning of the topics. Effectiveness of the tutorials was evaluated using quantitative methods and quasi-experimental design to compare the student learning results between the control year (without tutorials) and the year when tutorials were offered. Pre- and posttests measuring conceptual understanding were administered to assess gains in student learning. Additionally, student engagement was measured using the Classroom Survey of Student Engagement (CLASSE), and data from learning management system was collected. Findings: Results of the study show that the tutorials were an effective means of providing supplementary assistance to students as well as fostering a gain in students' levels of engagement with the course. Data analysis indicates that there was a significant increased gain in learning of core concepts in biology. Specifically, using formative online assessments resulted in measurable learning gains in students who participated voluntarily, in comparison to students who chose not to engage in self-paced quiz testing. Originality/value: As seen from the description earlier, the tutorials, and this project, provide suitable university-level complexity to address specific learning gaps in the first year course. They provide a valuable service to students in terms of representing content in an alternate format and motivating students as they engaged with videos and self-assessment most frequently. The project adds to the teaching and learning environment with respect to program design, mode of delivery and scheduling by providing self-paced tutorials that focus on specific concepts in biology. Students may review these resources whenever and as often as they feel necessary to better master the concepts. This makes the content applicable for the various preferences for approaches to learning and accommodation requirements found in students. Importantly, using formative online assessments resulted in measurable learning gains in students who participated voluntarily, in comparison to students who chose not to engage in self-paced quiz testing. [ABSTRACT FROM AUTHOR]

Riverine systems and associated fish populations worldwide are threatened by human impacts, especially in tropical countries with emerging economies. In India, community-based fish sanctuaries are a key mechanism for the conservation of freshwater fish populations, but there are few peer-reviewed studies on this subject. Here we integrate over 35 combined years of field experience with a literature synthesis to define and classify community-based fish sanctuaries. We present a novel, critical analysis of fish sanctuaries as social–ecological systems with a functional characterization based on natural capital, ecosystem services, human well-being, and policy and governance. We find that such sanctuaries are shaped by complex social–ecological processes, including coevolution of religious practices and ecological change, feedback processes created by retaliatory conflicts between river users, and diverse and dynamic governance strategies. These sanctuaries hold great potential for the conservation of rare fish species in India, but are subject to myriad threats at local, regional and global scales. Given the complexity of these social–ecological systems, we outline their conservation potential and highlight directions for future research. [ABSTRACT FROM AUTHOR]

Urbanization of natural landscapes and increasing human populations have brought people and our companion animals into closer contact with wildlife, even within protected areas. To provide guidance for human–wildlife coexistence, it is therefore critical to understand the effects of anthropogenic disturbances and how well native wildlife species survive in human-dominated landscapes. We investigated the spatio-temporal responses of 10 vertebrate taxa, with an emphasis on the Endangered Eld's deer Rucervus eldii thamin , to anthropogenic disturbances in Shwesettaw Wildlife Sanctuary, Myanmar. We quantified anthropogenic disturbances as distance from human settlements, distance from a highway, and the presence of people and free-ranging dogs Canis familiaris. Anthropogenic disturbances had stronger negative impacts on the detection of native wildlife species than on occupancy. Eld's deer avoided areas close to human settlements and showed low diel activity overlap with both people and dogs, although we found a positive association with human presence at the camera-trap sites. Five species exhibited lower diel activity overlap with people in the rainy season when human activity is the highest in our study area. All studied wildlife species shifted to nocturnal activity or did not show any clear activity pattern during the cool-dry season when the presence of dogs increased. The ecological and conservation impacts of dogs are underestimated in South-east Asia, particularly in Myanmar, and this case study highlights the impacts of dogs on the temporal use of habitat by wildlife and the need for better practices in the management of dogs within protected areas. [ABSTRACT FROM AUTHOR]

The symbiosis between ectomycorrhizal (ECM) fungal species and the root system of Pinus massoniana is important for its growth. However, the characteristics of ECM fungal communities and their relationships with extracellular enzyme activities and nutrients in the rhizosphere soil of different P. massoniana genotypes have not been well characterized. In this study, P. massoniana families (groups of offspring from different parents) with different levels of growth were selected for investigating ECM fungal communities, soil nutrients, extracellular enzyme activity, and leaf nutrient concentrations to explore the relationships between P. massoniana and the composition of the ECM fungal community. The high-growth (HG) family of P. massoniana had more different ECM fungal communities than the medium-growth (MG) and low-growth (LG) families; each family had a unique and dominant genera (HG: Amphinema and Pseudoclathrosphaerina; MG: Russula and Auricularia; and LG: Russula and Amanita). Amphinema was the main contributor to the differences among the three families (contribution: HG-MG 0.225 and HG-LG 0.17) and had rich extramatrical mycelium, which favored the growth of the HG family and positively affected the accumulation of soil organic carbon. Structural equation modelling showed that the dominant genera in the HG family had significant positive effects on the activity of three extracellular enzymes (BG, NAG, and AP) (weak to moderate positive effects of Amphinema on BG, NAG, and AP and moderate positive effects of Pseudoclathrosphaerina on BG, NAG, and AP), which might have contributed to the differences in extracellular enzyme activities among the families with different growth levels. Redundancy analysis indicated that P. massoniana growth traits (tree height, diameter at breast height, and timber volume), soil total nitrogen, and the N/P ratio significantly influenced ECM fungal communities. The study revealed the characteristics of ECM fungal communities, soil extracellular enzyme activity, and nutrient features of P. massoniana with different growth levels, which help improve our understanding of the relationship between P. massoniana genotype and ECM fungal communities. [ABSTRACT FROM AUTHOR]

Phosphorus (P) fertilizer is commonly used in subtropical plantations to augment nutrients including carbon (C), nitrogen (N), and P to maintain plants engaged in metabolism. Stoichiometric homeostasis reflects the adaptation of plants to various environments (including P fertilizer supply rates). It is thus of great significance to understand C:N:P stoichiometry in the plant–litter–soil system under P addition and the stoichiometric homeostasis of plant tissues for the P fertilization management of slash pine (Pinus elliottii Engelm) plantations. In subtropical China, we measured the C, N, and P contents in root, branch, needle, litter, and soil in slash pine plantations fertilized with four treatments, P1 (25 kg P ha1 yr1), P2 (50 kg P ha1 yr1), P3 (100 kg P ha1 yr1), and a control (CK), and calculated the stoichiometric homeostasis of plant tissues. The results show that P3 treatment increased the C, N, and P contents of the needle. P2 and P3 treatments increased the P content of the litter and the N:P ratio of the root while decreasing the C:N ratio of the root. P addition treatments increased C and P element accumulation in soil but had no effect on soil stoichiometry. The nutrient contents of needle and branch were higher than those of root and litter, indicating that slash pine was more inclined to allocate nutrients to the aboveground tissues. The stoichiometric homeostasis of C, N, and P among plant tissues was graded as follows: root > branch > needle. The needle's nutritional homeostasis was C > N > P, with 1/H values of 0.08, 0.34, and 0.74, respectively. These findings demonstrate that during P addition, the C, N, and P stoichiometric homeostasis varied among plant tissues and element types. In conclusion, P application altered nutrient distribution in the plant–litter–soil system, alleviating P restriction in slash pine forests in southern China. P addition levels should be finely adjusted in the future for longer-term observation trials, and the trade-off between P addition rates and economic and ecological advantages should be properly examined. [ABSTRACT FROM AUTHOR]

Rickettsiae of the spotted fever group (SFG) are zoonotic tick-borne pathogens. Small mammals are important hosts for the immature life stages of two of the most common tick species in Europe, Ixodes ricinus and Dermacentor reticulatus. These hosts and vectors can be found in diverse habitats with different vegetation types like grasslands and forests. To investigate the influence of environmental and individual factors on Rickettsia prevalence, this study aimed to analyse the prevalence of SFG rickettsiae in ticks and small mammals in different small-scale habitats in central Germany for the first time. Small mammals of ten species and ticks of two species were collected from grasslands and forests in the Hainich-Dün region, central Germany. After species identification, DNA samples from 1098 ticks and ear snips of 1167 small mammals were screened for Rickettsia DNA by qPCR targeting the gltA gene. Positive samples were retested by conventional PCR targeting the ompB gene and sequencing. Rickettsia DNA was detected in eight out of ten small mammal species. Small mammal hosts from forests (14.0%) were significantly more often infected than those from grasslands (4.4%) (p < 0.001). The highest prevalence was found in the mostly forest-inhabiting genus Apodemus (14.8%) and the lowest in Microtus (6.6%), which inhabits grasslands. The prevalence was higher in D. reticulatus (46.3%) than in the I. ricinus complex (8.6%). Adult ticks were more often infected than nymphs (p = 0.0199). All sequenced rickettsiae in I. ricinus complex ticks were R. helvetica, and the ones in D. reticulatus were R. raoultii. Unlike adults, questing nymphs have had only one blood meal, which explains the higher prevalence in I. ricinus adults. Interestingly, habitat type did influence infection probability in small mammals, but did not in ticks. A possible explanation may be the high prevalence in Apodemus flavicollis and A. sylvaticus which were more abundant in the forest. [ABSTRACT FROM AUTHOR]

The Indian rock pythons (Python molurus) are classified as a near-threatened snake species by the International Union for the Conservation of Nature and Natural Resources (IUCN); they are native to the Indian subcontinent and have experienced population declines caused primarily by poaching and habitat loss. We hand-captured the 14 rock pythons from villages, agricultural lands, and core forests to examine the species' home ranges. We later released/translocated them in different kilometer ranges at the Tiger Reserves. From December 2018 to December 2020, we obtained 401 radio-telemetry locations, with an average tracking duration of (444 ± 212 days), and a mean of 29 ± SD 16 data points per individual. We quantified home ranges and measured morphometric and ecological factors (sex, body size, and location) associated with intraspecific differences in home range size. We analyzed the home ranges of rock pythons using Auto correlated Kernel Density Estimates (AKDE). AKDEs can account for the auto-correlated nature of animal movement data and mitigate against biases stemming from inconsistent tracking time lags. Home range size varied from 1.4 ha to 8.1 km2 and averaged 4.2 km2. Differences in home range sizes could not be connected to body mass. Initial indications suggest that rock python home ranges are larger than other pythons. [ABSTRACT FROM AUTHOR]

Science-based conservation of riverine fishes can be best targeted with specific information about spatial-ecological controls on the community, including anthropogenic stressors. Because anthropogenic stressors can originate at multiple spatial scales, we investigated the influence of natural and anthropogenic variables summarized within the reach, valley, and catchment on fish community composition along four river mainstems in Ontario, Canada. We used Redundancy Analyses (RDA) to explore models with multi- and single-scale variables on fish community composition. We used partial RDAs to differentiate the relative effects of variable types in multiscale models and to determine if spatial variables explained additional variation in fish community composition. Catchment variables accounted for the majority of explained variation in fish community composition in three of the four rivers, but instream habitat variables accounted for considerable variability in fish community composition in the two rivers that are highly fragmented by dams or naturally occurring rapids. Natural and human-derived fragmentation in rivers may reduce the influence of catchment controls, disrupt longitudinal gradients, and increase the influence of local instream habitat. Environmental variables that explained fish distribution had longitudinal or patchy spatial pattern within rivers, but spatial variables representing impediments to fish dispersal and proximity to receiving waterbodies failed to explain additional variation in fish community composition. [ABSTRACT FROM AUTHOR]

Microbiome data is high dimensional, sparse, compositional, and over-dispersed. Therefore, modeling microbiome data is very challenging and it is an active research area. Microbiome analysis has become a progressing area of research as microorganisms constitute a large part of life. Since many methods of microbiome data analysis have been presented, this review summarizes the challenges, methods used, and the advantages and disadvantages of those methods, to serve as an updated guide for those in the field. This review also compared different methods of analysis to progress the development of newer methods. [ABSTRACT FROM AUTHOR]

Sexual conflict over mating may induce male mate-guarding prolonged in time in the absence of males, via devices such as mating plugs, widespread in insects. In most Lepidoptera, internal plugs are common, while in some butterfly families large external devices, called sphragides, evolved independently. Lack of, or incomplete sphragis in a few individuals were reported in sphragis-bearing species. Previous studies focused on typifying species-specific devices in a few specimens of many species. In contrast, we investigated alternative mate-guarding devices in detail of a sphragis-bearing butterfly. We conducted a six-year observational field study in a Clouded Apollo Parnassius mnemosyne population. Inspecting 492 females, we identified 3 different devices, filament, stopple and shield (i.e., sphragis) increasing in size and structure complexity, implying differential male investment and effectiveness in securing paternity. Shield dimensions, colour and all devices' shapes varied considerably. Shields were far more frequent than other devices. Some devices were lost, and a few were video-recorded when removed by males, showing the role of different parts of male external genitalia. We discuss potential causes of device variation and the role of removal attempts, and assess potential costs and benefits for both sexes. [ABSTRACT FROM AUTHOR]

Understanding how environmental gradients shape the spatial patterns of intraspecific genetic diversity is a central issue in ecological and evolutionary sciences. In riverine ecosystems, there is generally an increase in neutral genetic diversity downstream, as well as an increase in genetic differentiation among upstream populations. However, selective pressures may vary markedly along the upstream–downstream gradient, which could modify these patterns, but this has rarely been tested empirically. Here, we investigated how environmental gradients in a river network could shape the spatial patterns of intraspecific genetic diversity and differentiation in both neutral SNP markers and functional genetic markers putatively under natural selection (candidate SNPs associated with physiological functions and immune Major Histocompatibility Complex (MHC) loci) in wild brown trout populations. First, we showed that both the distance from the confluence and the centrality on the river network could explain the variation in genetic diversity and differentiation. Second, we found that both neutral and functional markers followed a similar pattern, with a higher genetic diversity and a lower genetic differentiation among populations that were more central and/or near to the confluence. This study highlights the importance of considering both the spatial and hydrological factors of a river network to understand and predict the role of dendritic connectivity in the spatial patterns of genetic diversity and differentiation in wild fish populations. [ABSTRACT FROM AUTHOR]

Habitat loss, and subsequent fragmentation, can increase the amount of forest edge. Primate species vary in their responses to such changes in their habitat. We studied the movement ecology of a group of reddish-brown cuxiú monkeys (Chiropotes sagulatus, Pitheciidae) in a small (13 ha), isolated forest remnant that was <3% of the species' typical home range in the region. Every 5 min we recorded the group's latitude and longitude, and the behavior of all individuals in sight. We calculated distance, speed, and trajectory of travel routes, and distance from every location to the nearest forest edge. Using Lidar data, we calculated canopy height. Cuxiús traveled in all cardinal directions, but they turned left more often than right, and they traveled at a faster speed to locations where the monkeys fed than where they did not eat. Although cuxiús used forest from the edge to the interior, they concentrated their movements and activities >30 m from the forest edge. Furthermore, their food sites were located less often near the forest edge, and canopy height of food trees near the forest edge was lower than canopy height of food trees at a greater distance from the edge. Although edge effects impacted the monkeys' movement, trees >15 m at the forest edge can provide resources. Future research can examine ecological variables in more detail with the movement patterns. [ABSTRACT FROM AUTHOR]

The sagebrush biome within the western United States has been reshaped by disturbances, management, and changing environmental conditions. As a result, sagebrush cover and configuration have varied over space and time, influencing processes and species that rely on contiguous, connected sagebrush. Previous studies have documented changes in sagebrush cover, but we know little about how the connectivity of sagebrush has changed over time and across the sagebrush biome. We investigated temporal connectivity patterns for sagebrush using a time series (1985–2020) of fractional sagebrush cover and used an omnidirectional circuit algorithm to assess the density of connections among areas with abundant sagebrush. By comparing connectivity patterns over time, we found that most of the biome experienced moderate change; the amount and type of change varied spatially, indicating that areas differ in the trend direction and magnitude of change. Two different types of designated areas of conservation and management interest had relatively high proportions of stable, high-connectivity patterns over time and stable connectivity trends on average. These results provide ecological information on sagebrush connectivity persistence across spatial and temporal scales that can support targeted actions to address changing structural connectivity and to maintain functioning, connected ecosystems. [ABSTRACT FROM AUTHOR]

Chemometric analysis is often used as an effective indicator of the supply capacity of nutrients in soil–plant systems and their biogeochemical cycles. Understanding ecological stoichiometric characteristics of C, N and P in soils under various land uses is crucial to guide ecological restoration and agricultural cultivation in karst rocky desertification region. However, data on ecological stoichiometry at different land uses in karst areas is limited. This study aimed to evaluate the effects of different land uses on soil ecological stoichiometric ratios and further identify the factors that influence soil ecological stoichiometric ratios. The topsoil from forest, shrub and cultivated land (paddy field and dry land) both in a karst area and non-karst area (as a reference) of Mashan County was sampled to investigate the spatial variance of the ecological stoichiometric characteristics of C, N and P under different land uses. The results show that: (1) Land-use types significantly determined the spatial heterogeneity of soil ecological stoichiometry in karst areas. (2) Soil organic carbon (SOC) was not significantly different between shrubs in the karst area and forests in the non-karst area (p = 0.595), but there were virtual differences in total nitrogen (TN), total phosphorus (TP), C:N, C:P and N:P between shrubs in the karst area and forests in the non-karst area (p < 0.01). (3) The contents of SOC, TN, and C:P, N:P in the study area were all generally higher in forests than those in cultivated land, and the content of TP was lower in forests than cultivated land, while C:N in cultivated land was higher than in shrubs in karst areas, and C:N was higher in forests than in cultivated land in non-karst areas. (4) Available nitrogen (AvN) was the main factor influencing stoichiometry in shrubs in karst areas, while pH, AvN, available phosphorus and elevation were the main factors in forests in non-karst areas, indicating that these factors significantly affect the soil ecological stoichiometric ratio during land-use changes. This study helps to understand the variations in soil ecological stoichiometric ratios under land-use changes. It provides guidance for the sustainable management of revegetation in karst regions in southwest China. [ABSTRACT FROM AUTHOR]

Burrowing rodents display diverse patterns of social organization, ranging from solitary to group living. Differences in social organization are often inferred from patterns of space use, particularly differences in the degree to which individual home ranges overlap. Here, we characterize patterns of space use in a poorly studied species of burrow-dwelling echimyid rodent, the Broad-headed Spiny Rat (Clyomys laticeps). Specifically, we use radiotelemetry studies of a free-living population in Parque Estadual da Serra de Caldas Novas (Goiás, Brazil) to evaluate previous, apparently contradictory reports suggesting that this species is both solitary and social. A total of 20 adult (12 females, 8 males) C. laticeps were monitored via telemetry during two consecutive cycles of annual wet and dry seasons. The resulting data indicate that although overlap of individual home ranges was common, co-occurrence of adults in time and space was rare and was limited to male–female pairs, a pattern that is most consistent with a solitary lifestyle in which individuals occupy distinct burrow systems. Neither sex nor season affected spatial overlap; this outcome is consistent with evidence suggesting that reproduction in this species occurs throughout the year. Although home ranges for males were larger than those for females, areas occupied by both males and females overlapped with multiple opposite-sex individuals, making it difficult to determine from spatial data whether the study population was polygynous or polygynandrous. While genetic data are needed to characterize fully the mating system of these animals, our analyses provide the first detailed information regarding spatial relationships in C. laticeps , thereby allowing more accurate placement of this species within a larger, comparative behavioral framework and facilitating efforts to identify factors contributing to social diversity among burrow-dwelling rodents. [ABSTRACT FROM AUTHOR]

The study of animal space use is fundamental to effective conservation and management of wildlife populations and habitats in a rapidly changing world, yet many species remain poorly described. Such is the case for the spatial ecology of the Vicuña–a medium-sized wild camelid that plays a critical role, both as a consumer and as prey, in the high Andean food web. We studied patterns of space use of 24 adult female vicuñas from April 2014 to February 2017 at the southern edge of its range. Vicuñas showed strong fidelity to their home range locations across the study period and shared large portions of their home ranges with vicuñas from other family groups. Vicuña home ranges in our study were considerably larger than previous estimates across the range of the species. Variation in environmental and terrain factors and the associated risk of predation affected vicuña diel migration distance but not home range size or overlap. Our study offers new ecological insights into vicuña space use that can inform conservation and management efforts of vicuñas and other social ungulates. [ABSTRACT FROM AUTHOR]

Pumas (Puma concolor) are solitary large carnivores that exhibit high energetic investments while hunting prey that often take multiple days to consume. Therefore, pumas should behave in a way to maximize their energetic gains, including using caching, which is a behavior used by many mammal species to preserve and store food or to conceal it from conspecifics and scavengers to limit their losses. Yet pumas do not always cache their kills. In order to understand caching behavior, we used variables associated with the kills such as prey mass, search time, climate, and habitat to test 20 ecological models (representing four a priori hypotheses: food perishability, resource pulse, consumption time, and kleptoparasitism deterrence) in an information-theoretic approach of model selection to explore factors related to the caching behavior. Models were run with information from tracked radio-collared pumas in California over a 2.5-year period and identified a total of 352 kills. Overall, we documented pumas caching 61.5% of their kills, including 71.6% of Black-tailed Deer (Odocoileus hemionus columbianus), their primary prey in the study area. The model with a quadratic effect of adjusted mass of prey on puma caching probability had all of the empirical support (w = 1.00). Specifically, pumas were most likely to cache intermediate-sized prey, such as yearling and adult female deer, and also fed from cached kills for longer periods of time. Larger prey may be too large to easily cache, making it less energetically efficient—while small prey can often be consumed quickly enough to not require caching. This suggests that intermediate-sized prey may be the optimal size for caching, allowing a puma to feed for multiple days while not greatly increasing energetic output. The hypotheses we tested were not mutually exclusive and pumas caching their prey may occur for several reasons; nevertheless, our study demonstrated that pumas use caching to extend their foraging time and maximize energetic gains when preying on intermediate-sized prey. [ABSTRACT FROM AUTHOR]