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Diabetes mellitus (DM) is a chronic inflammatory disease, and the rapidly increasing DM is becoming a major problem of global public health. Traditional Chinese medicine (TCM) has a long history of treating diabetes. It has been developed and utilized because of its good efficacy and no toxic side effects. Lobelia chinensis is a traditional whole grass herbal. With the continuous deepening of pharmacological research on TCM, the active ingredients of L. chinensis are continuously revealed, which contained the alkaloids, flavonoids, flavonoid glycosides and amino acids that have the good effects of anti-inflammatory, anti-viral and anti-diabetic. In order to further explore the targets of active ingredients and its anti-diabetic mechanism, a feasible network pharmacology analysis model based on chemical, pharmacokinetic and pharmacological data was developed by network construction method to clarify the anti-diabetic mechanism of L. chinensis. The present study conducted by gas chromatography–mass spectrometer (GC/MS), which identified 208 metabolites of L. chinensis, of which 23 ingredients may have effective pharmacological effects after absorption, distribution, metabolism, and excretion (ADME) screening. Network pharmacological analysis on the active ingredients revealed that 5-hydroxymethylfurfural in L. chinensis affects the insulin resistance signaling pathway by acting on GSK3B, TNF, and MAPK1, acacetin affects the diabetic pathway by acting on INSR, DPP4, and GSK3B, that regulate type 2 diabetes, non-insulin-dependent DM, and inflammatory diseases. These results successfully indicated the potential anti-diabetic mechanism of the active ingredients of L. chinensis.

A recently developed pneumonia caused by SARS-CoV-2 bursting in Wuhan, China, has quickly spread across the world. We report the clinical characteristics of 82 cases of death from COVID-19 in a single center. Clinical data on 82 death cases laboratory-confirmed as SARS-CoV-2 infection were obtained from a Wuhan local hospital's electronic medical records according to previously designed standardized data collection forms. All patients were local residents of Wuhan, and a large proportion of them were diagnosed with severe illness when admitted. Due to the overwhelming of our system, a total of 14 patients (17.1%) were treated in the ICU, 83% of deaths never received Critical Care Support, only 40% had mechanical ventilation support despite 100% needing oxygen and the leading cause of death being pulmonary. Most of the patients who died were male (65.9%). More than half of the patients who died were older than 60 years (80.5%), and the median age was 72.5 years. The bulk of the patients who died had comorbidities (76.8%), including hypertension (56.1%), heart disease (20.7%), diabetes (18.3%), cerebrovascular disease (12.2%), and cancer (7.3%). Respiratory failure remained the leading cause of death (69.5%), followed by sepsis/MOF (28.0%), cardiac failure (14.6%), hemorrhage (6.1%), and renal failure (3.7%). Furthermore, respiratory, cardiac, hemorrhagic, hepatic, and renal damage were found in 100%, 89%, 80.5%, 78.0%, and 31.7% of patients, respectively. On admission, lymphopenia (89.2%), neutrophilia (74.3%), and thrombocytopenia (24.3%) were usually observed. Most patients had a high neutrophil-to-lymphocyte ratio of >5 (94.5%), high systemic immune-inflammation index of >500 (89.2%), and increased C-reactive protein (100%), lactate dehydrogenase (93.2%), and D-dimer (97.1%) levels. A high level of IL-6 (>10 pg/ml) was observed in all detected patients. The median time from initial symptoms to death was 15 days (IQR 11-20), and a significant association between aspartate aminotransferase (p = 0.002), alanine aminotransferase (p = 0.037) and time from initial symptoms to death was remarkably observed. Older males with comorbidities are more likely to develop severe disease and even die from SARS-CoV-2 infection. Respiratory failure is the main cause of COVID-19, but the virus itself and cytokine release syndrome-mediated damage to other organs, including cardiac, renal, hepatic, and hemorrhagic damage, should be taken seriously as well.

Using discrete element software, namely, particle flow code as two-dimensional program (PFC2D), two types of models were established: vertical fissure hole combination and horizontal fissure hole combination with ratios of major and minor axis of ellipse being 1, 1.2, 1.5, 2, and 3, which corresponded to a total of ten samples. The failure mode, mechanical behavior, and stress state before and after crack generation in elliptical hole crack combination models with different ratios of major and minor axis were analyzed. The crack development, stress field evolution, and acoustic emission characteristics of the vertical fissure model and horizontal fissure model were studied at the optimized ratio of major and minor axis of ellipse being 1.5. The results showed that elliptical hole fissure with different ratios of major and minor axis resulted in the decrease in the strength and elastic modulus of rock and increase in the peak strain of rock. The effect of the horizontal fissure model on the peak strength, peak strain, and elastic modulus of rock was found to be greater than that of the vertical fissure hole model. Ellipses with different ratios of major and minor axis in various models slightly influenced the rock failure modes, and their failure modes corresponded to tensile shear failure and tensile failure. Before crack formation, the tensile stress concentration areas of each model were, respectively, distributed at the upper and lower ends of the vertical fissure and the major axis of ellipse, and the compressive stress concentration areas were distributed at both ends of the major axis of ellipse and the fissure in the horizontal direction. After the model failed, the compressive stress concentration areas of the vertical fissure model and the horizontal fissure model transferred to the left upper part and the right upper part of the model along the left end of the hole and the right end of the fissure, respectively. When the ratio of major and minor axis of ellipse was 1.5, cracks in the vertical model and the horizontal model of fissure developed along the axial direction at the ends of cracks and holes, respectively, and then secondary cracks were generated at the ends of left and right sides. The maximum compressive stress in each stage of the vertical fissure model was greater than that of the horizontal fissure model, and when the model was damaged, its stress release was more.

Hyperspectral images can capture subtle differences in reflectance of features in hundreds of narrow bands, and its pixel-wise classification is the cornerstone of many applications requiring fine-grained classification results. Although three-dimensional convolutional neural networks (3D-CNN) have been extensively investigated in hyperspectral image classification tasks and have made significant breakthroughs, hyperspectral classification under small sample conditions is still challenging. In order to facilitate small sample hyperspectral classification, a novel mixed spatial-spectral features cascade fusion network (MSSFN) is proposed. First, the covariance structure of hyperspectral data is modeled and dimensionality reduction is conducted using factor analysis. Then, two 3D spatial-spectral residual modules and one 2D separable spatial residual module are used to extract mixed spatial-spectral features. A cascade fusion pattern consisting of intra-block feature fusion and inter-block feature fusion is constructed to enhance the feature extraction capability. Finally, the second-order statistical information of the fused features is mined using second-order pooling and the classification is achieved by the fully connected layer after L2 normalization. On the three public available hyperspectral datasets, Indian Pines, Houston, and University of Pavia, only 5%, 3%, and 1% of the labeled samples were used for training, the accuracy of MSSFN in this paper is 98.52%, 96.31% and 98.83%, respectively, which is far better than the contrast models and verifies the effectiveness of MSSFN in small sample hyperspectral classification tasks.

Abstract Background Existing data about the prognostic value of absolute count of blood cells in gastric cancer was limited. Thus, the present study aims to investigate the prognostic value of absolute count of white blood cell (WBC), neutrophil, lymphocyte, monocyte and platelet in gastric cancer patients. Methods From September 2008 to March 2015, 3243 patients treated with radical gastrectomy were enrolled in the present study. Clinicopathological characteristics were recorded. The prognostic value of blood test in gastric cancer patients were analyzed. Results There were 2538 male and 705 female. The median age was 58 years (range 20–90). The median follow-up time was 24.9 months (range 1–75). The 1-, 3- and 5-year overall survival rate was 88.9%, 65.8% and 57.2%, respectively. The optimal cut off value was 6.19 × 109/L for WBC (P = 0.146), 4.19 × 109/L for neutrophil (P = 0.004), 1.72 × 109/L for lymphocyte (P = 0.000), 0.51 × 109/L for monocyte (P = 0.019) and 260.0 × 109/L for platelet (P = 0.002), respectively. Neutrophil, lymphocyte, monocyte and platelet were risk factors for the prognosis of gastric cancer (all P

The dual active bridge (DAB) converter is gaining more and more attention in various applications such as energy storage systems, electric vehicles and smart grids. To improve the quality of the input current, a LC filter is often cascaded at the input side of the DAB converter. However, there are instable problems of this cascaded system due to the impedance interactions of the DAB converter and the LC filter, although the DAB converter is stable at the individual operation mode. To assess the stability of the cascaded system of the DAB converter and the LC filter, the impedance model of the DAB converter is firstly developed based on generalized state-space averaging method. The developed impedance model can be used to accurately predict the stability of the DAB converter with its LC input filter. Based on the stability analysis, the optimum filter parameter design guideline is determined. The impedance model and stability analysis are validated by the simulation and experimental results.

Abstract Background Up to date, investigation of the prognostic value of differentiation status mainly focused on signet ring cell and mucinous gastric cancer. Thus, the present study aims to investigate the clinicopathological features and prognosis of gastric cancer patients with well, moderately and poorly differentiation status. Methods From September 2008 to March 2015, a total of 3090 gastric cancer patients treated with radical D2 gastrectomy were enrolled in the present study. Clinicopathological characteristics and prognosis of gastric cancer patients with well, moderately and poorly differentiation status were analyzed. Results There were 2422 male (78.4%) and 668 female (21.6%). The median age was 58 (20–90) years. There were 370 (12.0%) well differentiated tumors, 836 (27.0%) moderately differentiated tumors and 1884 (61.0%) poorly differentiated tumors. Well and moderately differentiation status were associated with older age, male gender, smaller tumor, shallower invasion, less lymph node involvement and earlier tumor stage (all p

Abstract Background MicroRNAs are small RNAs (~ 22 nt) that modulate the expression of thousands of genes in tumors and play important roles in the formation of multidrug resistance. In this study, we firstly investigated that miR-4532 involved in the multidrug resistance formation of breast cancer by targeting hypermethylated in cancer 1 (HIC-1), a tumor-suppressor gene. Methods To identify and characterize the possible miRNAs in regulating multidrug resistance, we employed the transcriptome sequencing approach to profile the changes in the expression of miRNAs and their target mRNAs were obtained by bioinformatics prediction. Then the molecular biology experiments were conducted to confirm miR-4532 involved in multidrug resistance formation of breast cancer. Results The luciferase reporter assay experiment was employed to confirm that HIC-1 was the target of miR-4532. Transfection with an miR-4532 mimic indicated miR-4532 mimic significantly increased breast cancer cell resistance to adriamycin. Cell proliferation and invasion assay experiments showed overexpression of HIC-1 inhibited the invasion and metastasis of breast cancer cells. Meanwhile, the interleukin (IL)-6/signal transducer and activator of transcription 3 (STAT3) signaling pathway was confirmed to be involving in multidrug resistance by western blotting experiments. Conclusions These results suggest that downregulation of hypermethylated in cancer-1 by miR-4532 could promote adriamycin resistance in breast cancer cells, in which the IL-6/STAT3 pathway was regulated by the HIC-1. This finding might contribute to new therapeutic target for reversal of tumor resistance.

Autophagy, a self-digestion intracellular catabolic process, plays a crucial role in cellular homeostasis under conditions of starvation, oxidative stress and genotoxic stress. The capability of maintaining homeostasis contributes to preventing malignant behavior in normal cells. Many studies have provided compelling evidence that autophagy is involved in brain tumor recurrence and chemotherapy and radiotherapy resistance. Gliomas, as the primary central nervous system (CNS) tumors, are characterized by rapid, aggressive growth and recurrence and have a poor prognosis and bleak outlook even with modern multimodality strategies involving maximal surgical resection, radiotherapy and alkylating agent-based chemotherapy. Autophagy-associated signaling pathways, such as the extracellular signal-regulated kinase1/2 (ERK1/2) pathway, class I phosphatidylinositol 3-phosphate kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway and nuclear factor kappa-B (NF-κB) pathway, act as tumor suppressors or protect tumor cells against chemotherapy/radiotherapy-induced cytotoxicity in gliomagenesis. Through these pathways, both lethal autophagy and protective autophagy play crucial roles in tumor initiation, chemoresistance and glioma stem cell differentiation. Moreover, lethal autophagy and protective autophagy have been identified as novel therapeutic targets in glioma according to the mechanisms described above. Here, we discuss the multiple impacts of the autophagic response on distinct phases of gliomagenesis and the advanced progress of therapies based on this concept.

Mesenchymal stromal cells (MSCs) based therapy is a promising approach to treat inflammatory disorders. However, therapeutic effect is not always achieved. Thus the mechanism involved in inflammation requires further elucidation. To explore the mechanisms by which MSCs respond to inflammatory stimuli, we investigated whether MSCs employed inflammasomes to participate in inflammation. Using in vitro and in vivo models, we found that canonical NLRP3 and non-canonical caspase-11 inflammasomes were activated in bone-associated MSCs (BA-MSCs) to promote the inflammatory response. The NLRP3 inflammasome was activated to mainly elicit IL-1β/18 release, whereas the caspase-11 inflammasome managed pyroptosis. Furthermore, we sought a small molecule component (66PR) to inhibit the activation of inflammasomes in BA-MSCs, which consequently improved their survival and therapeutic potential in inflammation bowel diseases. These current findings indicated that MSCs themselves could directly promote the inflammatory response by an inflammasome-dependent pathway. Our observations suggested that inhibition of the proinflammatory property may improve MSCs utilization in inflammatory disorders. Keywords: Mesenchymal stromal cells, Inflammasome, NLRP3, Caspase-11, Pyroptosis

Abstract Background Osteoporotic vertebral compression fracture (OVCF) is a common type of fracture, and percutaneous kyphoplasty (PKP) is an eligible solution to it. Previous studies have revealed that both the volume and filling pattern of bone cement correlate with the clinical outcomes after PKP procedure. However, the role of bone cement distribution remains to be illustrated. Methods To retrospectively evaluate the relationship between the bone cement distribution and the clinical outcomes of unilateral PKP, we enrolled 73 OVCF patients receiving unilateral PKP treatment. All the intervened vertebrae were classified into three groups based on the bone cement distribution observed on postoperative X-ray films. Preoperative and postoperative radiographic parameters including the vertebral height and kyphotic Cobb angle were recorded, and anterior vertebral height restoration rate (AVHRR) and Cobb angle correction (CR) were then calculated to assess the vertebral height reconstruction. Preoperative and postoperative Oswestry Disability Index (ODI) and visual analogue scale (VAS) were adopted by interviewing patients to assess the mobility improvement and pain relief. Demographic data, body mass index (BMI), lumbar bone mineral density (evaluated by BMD T-score) of each patient, bone cement volume (BV), and bone cement extravasation (BE) were also recorded. Between- and within-group comparisons and multivariable correlation analysis were carried out to analyze the data. Results VAS and ODI scores were both significantly improved in all of the enrolled cases with no significant differences between groups. Among the three groups, the average age, AVHRR, and BV were significantly different. Occurrence of BE was significantly different between two of the three groups. AVHRR was demonstrated to correlate negatively with preoperative anterior vertebral height ratio and positively with preoperative Cobb angle, CR, diffusion score, and ODI changes. Conclusions Bone cement distribution is a potential predictor to the reconstructive effects in unilateral PKP for OVCFs. Bone cement distribution is associated with AVHRR and BV, as well as the risk of BE occurrence. Greater bone cement distribution may indicate better vertebral restoration along with a higher BE risk.

Abstract Background The relatively low incidence of duodenal gastrointestinal stromal tumors (GISTs) and the unique anatomy make the surgical management and outcomes of this kind of tumor still under debate. Thus, this study aimed to explore the optimal surgical strategy and prognosis of duodenal GISTs. Methods A total of 300 cases of duodenal GISTs were obtained from our center (37 cases) and from case reports or series (263 cases) extracted from MEDLINE. Clinicopathological features, type of resections and survivals of duodenal GISTs were analyzed. Results The most common location of duodenal GISTs was descending portion (137/266, 51.5%). The median tumor size was 4 cm (0.1–28). Most patients (66.3%) received limited resection (LR). Pancreaticoduodenectomy (PD) was mainly performed for GISTs with larger tumor size or arose from descending portion (both P 0.05). Duodenal GISTs were significantly different from gastric GISTs with respect to tumor size, mitotic index and NIH risk category (all P

Abstract Background The association between body mass index (BMI) and clinical outcomes of gastric cancer were still under debate. The aim of the present study was to investigate the impact of BMI on intraoperative conditions, postoperative complications and prognosis of gastric cancer. Methods From October 2008 to March 2015, 1210 gastric cancer patients treated with D2 gastrectomy were enrolled in the present study. Patients were divided into three groups: low BMI group (BMI 0.05). High BMI was associated with increased blood loss and operation time, and deceased number of retrieved lymph nodes (all P 0.05). Conclusions BMI was inversely associated with tumor size, tumor depth, LNM and tumor stage. High BMI was associated with increased blood loss and operation time, and deceased number of retrieved lymph nodes. Low BMI was associated with decreased rate of postoperative fever and decreased survival.

A Zr-based stable metal-organic frameworks DUT-67 (Zr) was successfully synthesized as an adsorbent to remove trace mercury and methylmercury ions in aqueous solution. The removal efficiency of 90% and 55% of Hg2+ and CH3Hg+ was respectively achieved at pH 6 and 55°C. The S in thiophene has a relatively weak adsorption capacity for mercury and there could be the slight π-complexation between thiophere ring of DUT-67 (Zr) and Hg2+ besides physical absorption, while there only was physical adsorption between DUT-67 (Zr) and CH3Hg+. The developed methods were applied to remove trace Hg2+ and CH3Hg+ in the real water samples, and the removal efficiency was from 69% to 90% and from 30% to 77% respectively; when the concentrations of Hg2+ was lower than 20 μg L−1 in the samples, the remaining mercury concentration was lower than 1 μg L−1，which can meet the standard of the World Health Organization.

Visual object tracking is a challenging task when the object appearance changes caused by the scale variation and the occlusion. In this paper, an object tracking algorithm is proposed which is capable of dealing with the case that the scale variation and the occlusion occur simultaneously. A kernelized correlation filter (KCF) is first learned to obtain the correlation response, whose maximum value denotes the optimal object location. In order to represent the sample better, the convolutional features are extracted from a pre-trained convolutional neural networks (CNNs). Then, the strategy of scale adaption is used to estimate the object scale during the tracking process. Subsequently, a novel re-detection model is proposed by using a support vector machine (SVM) classifier to re-find the object when the occlusion occurs. The comparison experiments are implemented on the object tracking benchmark (OTB) and the results demonstrate that the proposed tracking algorithm outperforms other state-of-the-art ones in terms of precision and success rate.

In this investigation, the uniaxial short-term creep tests with multi-step loading were conducted on the sandstone-coal composite samples, and the characteristics of creep strength, creep deformation, acoustic emission (AE), and creep failure of composite samples were studied, respectively. The creep strength of the composite sample decreased with the stress-level duration, which was mainly determined by the coal and influenced by the interactions with the sandstone. The creep deformation and damage of sandstone weakened the deformation and damage accumulation within the coal, resulting in the larger strength for the composite sample compared with the pure coal sample. The axial creep strain of composite sample generally increased with the stress-level or the stress-level duration under same conditions. The AE characteristics of composite sample were related to the creep strain rate, the stress level, the stress level duration, and the local failure or fracture during creep loading. The micro or macro failure and fracture within the composite sample caused the rise in the axial creep strains and the frequency and intensity of AE signals, especially the macro failure and fracture. The creep failures of composite samples mainly occurred within the coal with the splitting ejection failure accompanied by the local shear failure, and no obvious failures were found within the sandstone. The coal in the composite sample became more broken with the stress-level duration.

Depression, anxiety, and insomnia are common in cancer patients. Mind-body therapies (MBTs) are promising forms of treatment for cancer patients living with depression, anxiety, and insomnia. The objective of this study is to assess the effectiveness and acceptability of MBTs in cancer patients living with depression, anxiety, or insomnia. EMBase, PubMed, Cinahl, PsychINFO, IndMED, CSI-NISCAIR, CNKI, Clinicaltrial.gov, ChiCTR, and CTRI will be searched until October 2020 for relevant studies. Randomized controlled studies in which MBTs were tested in a cancer population will be selected. The authors of the selected studies will be contacted to obtain individual participant data. The participants who reached a defined clinical threshold for depression, anxiety, or insomnia will be selected for the three sub-studies on depression, anxiety, and insomnia, respectively. Pairwise and network meta-analyses will be used to assess the changes in depression, anxiety, sleep quality, and completion rate. We will assess the effect of the treatment dose (number and frequency of interventions) on effectiveness. The results of this study will inform clinical decision-making for the treatment of psychological disturbances in cancer patients. If MBTs are found effective, they will potentially be recommended as treatments for cancer patients with psychological symptoms.

An electroencephalogram (EEG) is an electrophysiological signal reflecting the functional state of the brain. As the control signal of the brain–computer interface (BCI), EEG may build a bridge between humans and computers to improve the life quality for patients with movement disorders. The collected EEG signals are extremely susceptible to the contamination of electromyography (EMG) artifacts, affecting their original characteristics. Therefore, EEG denoising is an essential preprocessing step in any BCI system. Previous studies have confirmed that the combination of ensemble empirical mode decomposition (EEMD) and canonical correlation analysis (CCA) can effectively suppress EMG artifacts. However, the time-consuming iterative process of EEMD may limit the application of the EEMD-CCA method in real-time monitoring of BCI. Compared with the existing EEMD, the recently proposed signal serialization based EEMD (sEEMD) is a good choice to provide effective signal analysis and fast mode decomposition. In this study, an EMG denoising method based on sEEMD and CCA is discussed. All of the analyses are carried out on semi-simulated data. The results show that, in terms of frequency and amplitude, the intrinsic mode functions (IMFs) decomposed by sEEMD are consistent with the IMFs obtained by EEMD. There is no significant difference in the ability to separate EMG artifacts from EEG signals between the sEEMD-CCA method and the EEMD-CCA method (p > 0.05). Even in the case of heavy contamination (signal-to-noise ratio is less than 2 dB), the relative root mean squared error is about 0.3, and the average correlation coefficient remains above 0.9. The running speed of the sEEMD-CCA method to remove EMG artifacts is significantly improved in comparison with that of EEMD-CCA method (p < 0.05). The running time of the sEEMD-CCA method for three lengths of semi-simulated data is shortened by more than 50%. This indicates that sEEMD-CCA is a promising tool for EMG artifact removal in real-time BCI systems.

Although various polymer membrane materials have been applied to gas separation, there is a trade-off relationship between permeability and selectivity, limiting their wider applications. In this paper, the relationship between the gas permeation behavior of polyphenylsulfone(PPSU)-based materials and their chemical structure for gas separation has been systematically investigated. A PPSU homopolymer and three kinds of 3,3′,5,5′-tetramethyl-4,4′-biphenol (TMBP)-based polyphenylsulfone (TMPPSf) copolymers were synthesized by controlling the TMBP content. As the TMPPSf content increases, the inter-molecular chain distance (or d-spacing value) increases. Data from positron annihilation life-time spectroscopy (PALS) indicate the copolymer with a higher TMPPSf content has a larger fractional free volume (FFV). The logarithm of their O2, N2, CO2, and CH4 permeability was found to increase linearly with an increase in TMPPSf content but decrease linearly with increasing 1/FFV. The enhanced permeability results from the increases in both sorption coefficient and gas diffusivity of copolymers. Interestingly, the gas permeability increases while the selectivity stays stable due to the presence of methyl groups in TMPPSf, which not only increases the free volume but also rigidifies the polymer chains. This study may provide a new strategy to break the trade-off law and increase the permeability of polymer materials largely.

Abstract Background Patients suffering from advanced stage hepatocellular carcinoma (HCC) often exhibit a poor prognosis or dismal clinical outcomes due to ineffective chemotherapy or a multi-drug resistance (MDR) process. Thus, it is urgent to develop a new chemotherapeutic sensitivity testing system for HCC treatment. The presence study investigated the potential application of a novel chemotherapeutic sensitivity-testing system based on a collagen gel droplet embedded 3D–culture system (CD-DST). Methods Primary cells were separating from surgical resection specimens and then tested by CD-DST. To identify whether HCC cell lines or cells separating from clinical specimens contain MDR features, the cells were treated with an IC 50 (half maximal inhibitory concentration) or IC max (maximal inhibitory concentration) concentration of antitumor agents, e.g., 5-furuolouracil (5-FU), paclitaxel (PAC), cisplatin (CDDP), epirubicin (EPI), or oxaliplatin (L-OHP), and the inhibitory rates (IRs) were calculated. Results HepG2 cells were sensitive to 5-FU, PAC, CDDP, EPI, or L-OHP; the IC 50 value is 0.83 ± 0.45 μg/ml, 0.03 ± 0.02 μg/ml, 1.15 ± 0.75 μg/ml, 0.09 ± 0.03 μg/ml, or 1.76 ± 0.44 μg/ml, respectively. Only eight (8/26), nine (9/26), or five (5/26) patients were sensitive to the IC max concentration of CDDP, EPI, or L-OHP; whereas only three (3/26), four (4/26), or two (2/26) patients were sensitive to the IC 50 concentration of CDDP, EPI, or L-OHP. No patients were sensitive to 5-FU or PAC. Conclusions The in vitro drug sensitivity exanimation revealed the MDR features of HCC and examined the sensitivity of HCC cells from clinical specimens to anti-tumor agents. CD-DST may be a useful method to predict the potential clinical benefits of anticancer agents for HCC patients.

Abstract Background Data on the safety and feasibility of laparoscopic versus open resection for gastric gastrointestinal stromal tumors (GISTs) larger than 5 cm are limited. Therefore, the aim of this meta-analysis was to compared laparoscopic and open resection for gastric GISTs larger than 5 cm. Methods We perform a literature search on PubMed, the Cochrane Library, and Embase. Review Manage version 5.1 (RevMan 5.1) was used for data analysis. The GRADE profiler software (version 3.6) was used to estimate the level of evidence. Results A total of 6 observational studies and one unpublished retrospective cohort study met the inclusion criteria for the meta-analysis: 203 patients in LAP and 214 patients in OPEN group. The pooled result revealed that laparoscopic resection was associated with a same operative time (WMD = −0.87 min; 95% CI: -47.50 to 47.75; P = 0.97), intraoperative blood loss (WMD = −34.38 ml; 95% CI: -79.60 to 10.84; P = 0.14), overall complications (RR = 0.65; 95% CI: 0.38 to 1.12; P = 0.12), better 5-year disease-free survival (HR = 0.40; 95% CI: 0.17 to 0.91; P = 0.03) and overall survival (HR = 0.09; 95% CI: 0.02 to 0.40; P = 0.002) compared with open resection. Conclusion Laparoscopic resection is a technically and oncologically safe and feasible approach for large-sized gastric GISTs (≥ 5 cm) compared to open resection.

Abstract Background The diagnostic and prognostic significance of carcinoembryonic antigen (CEA), carbohydrate associated antigen 19–9 (CA19–9), alpha-fetoprotein (AFP) and cancer antigen 125 (CA125) in early gastric cancer have not been investigated yet. Thus, the present study aimed to explore the diagnostic and prognostic significance of the four tumor markers for early gastric cancer. Methods From September 2008 to March 2015, 587 early gastric cancer patients were given radical gastrectomy in our center. The clinicopathological characteristics were recorded. The association between levels of CEA and CA19–9 and clinicopathological characteristics and prognosis of patients were analyzed. Results There were 444 men (75.6%) and 143 women (24.4%). The median age was 57 years (ranged 21–85). The 1-, 3- and 5-year overall survival rate was 99.1%, 96.8% and 93.1%, respectively. The positive rate of CEA, CA19–9, AFP and CA125 was 4.3%, 4.8%, 1.5% and 1.9%, respectively. The positive rate of all markers combined was 10.4%. The associations between the clinicopathological features and levels of CEA and CA19–9 were analyzed. No significant association was found between CEA level and clinicopathological features. However, elevated CA19–9 level was correlated with female gender and presence of lymph node metastasis. Age > 60 years old, presence of lymph node metastasis and elevation of CEA level were independent risk factors for poor prognosis of early gastric cancer. Conclusions The positive rates of CEA, CA19–9, APF and CA125 were relatively low for early gastric cancer. Elevation of CA19–9 level was associated with female gender and presence of lymph node metastasis. Elevation of CEA level was an independent risk factor for the poor prognosis of early gastric cancer.

Abstract Background Vertebrae, ribs, and spinal cord are anatomically adjacent structures, and their close relationships are clinically important for planning better corrective surgical approach. The objective is to identify the radiographic characteristics in surgical patients with congenital scoliosis (CS) and coexisting split cord malformation (SCM). Methods A total of 266 patients with CS and SCM underwent surgical treatment at our hospital between May 2000 and December 2015 was retrospectively identified. The demographic distribution and radiographic data were collected to investigate the characteristics of spine curve, vertebral, rib, and intraspinal anomalies. According to Pang’s classification, all patients were divided into two groups: type I group is defined as two hemicords, each within a separate dural tube separated by a bony or cartilaginous medial spur, while type II group is defined as two hemicords within a single dural tube separated by a nonrigid fibrous septum. Results There were 104 patients (39.1%) in Type I group and 162 patients (60.9%) in Type II group. SCM was most commonly found in the lower thoracic and lumbar regions. The mean length of the septum in Type I SCM was significantly shorter than Type II SCM (2.7 vs. 5.2 segments). Patients in Type I group had a higher proportion of kyphotic deformity (22.1%). The vertebral deformities were simple in only 16.5% and multiple in 83.5% of 266 cases. Patients in Type I group presented higher prevalence of multiple (90.4%) and extensive (5.1 segments) malformation of vertebrae. In addition, hypertrophic lamina and bulbous spinous processes were more frequent in Type I group (29.7%), even developing into the “volcano-shape” deformities. Rib anomalies occurred in 62.8% of all patients and 46.1% of them were complex anomalies. The overall prevalence of other intraspinal anomalies was 42.9%. The most common coexisting intraspinal anomalies was syringomyelia (30.5%). Conclusion The current study, with the largest cohort to date, demonstrated that patients with CS and coexisting SCM presented high prevalence of multiple vertebral deformities, rib and intraspinal anomalies. The length of the split segment in Type I SCM was shorter than that in Type II SCM. Compared with Type II SCM, patients with Type I SCM presented with higher incidence of kyphotic deformity, more extensive and complicated vertebral anomalies, and more complex rib anomalies.

Abstract Osseointegration is the key to implant stability and occlusal support. Biomechanical response and remodeling of peri-implant bone occurs under impact loading. Sclerostin participates in bone formation and resorption through Wnt and RANKL pathways. However the mechanism of microdamage and expression of sclerostin in peri-implant bone under impact load is still unclear. In present study, specific impact forces were applied to the implants with favorable osseointegration in rabbits. The microdamage of peri-implant bone and the expression of sclerostin, β-catenin and RANKL during the process of bone damage and remodeling were investigated by micro-CT, histology, immunofluorescence and RT-qPCR analysis. Interface separation and trabecular fracture were found histologically, which were consistent with micro-CT analyses. Throughout remodeling, bone resorption was observed during the first 14 days after impact, and osseointegration and normal trabecular structure were found by 28 d. The expression of sclerostin and RANKL increased after impact and reached a maximum by 14 d, then decreased gradually to normal levels by 28 d. And β-catenin expression was opposite. Results indicated that sclerostin may involve in the peri-implant bone damage caused by impact and remodeling through Wnt/β-catenin and RANKL/RANK pathways. It will provide a new insight in the diagnosis and treatment for patients suffering impact.

Abstract Data about primary gastric adenosquamous carcinoma (ASC) was limited due to rare incidence. Thus, the present study aims to investigate clinicopathological features and prognosis of gastric ASC. Cases of gastric ASC were obtained from our center and from case reports and series extracted from Medline. Clinicopathological features and prognosis of gastric ASC were analyzed and compared with gastric adenocarcinoma (AC) in our center. The commonest location was lower third (45.0%), followed by upper (26.2%) and middle third (24.2%). The median tumor size was 6 cm (0.8–17). The commonest differentiation status was well for both AC (44.4%) and SCC components (40.9%). Half of tumors (52.7%) were stage T4 and most patients (86.2%) suffered from lymph node metastasis (LNM). Tumor depth and TNM stage were risk factors for overall survival (OS) (both P

Hyperspecral unmixing (HU) is one of the crucial steps of hyperspectral image (HSI) processing. The process of HU can be divided into end-member extraction and abundance estimation. Lots of abundance estimation methods just take some properties of abundance into consideration, such as non-negative, sum-to-one and so on but ignore the noise corruption. However, in practical applications, there are always high-noise bands in HSI due to water absorption, atmospheric transmission, and other inevitable factors, which lead to the estimation accuracy reduction. Here, we propose a new abundance estimation model which takes the mixing pattern of endmembers and low signal-to-noise ratio (SNR) bands of HSI into consideration simultaneously. The constraints considering not only the low-rank feature of abundance but also the sparsity quality of noise are imposed on the new model for more robust results. Adequate experiments both on synthetic and real hyperspectral data have confirmed the superiority of our method.

Hyperspectral image (HSI) classification is a hot topic in remote sensing community; many researchers have made a great deal of effort in this domain. Recently, deep learning-based manner paves a new way to better classification accuracy. However, the flow of information between layers and layers (e.g. max-pooling) in traditional deep architecture turns out to be ineffective. In this study, a novel spectral–spatial classification framework for HSI based on Capsule Network (CapsNet) and dynamic routing algorithm is introduced. The proposed architecture is composed of a hybrid of 1D and 2D convolutional layers and two capsule layers for better and effective mining and combining features. Consequently, experiments on two popular dataset indicate that CapsNet-based framework outperforms traditional CNN-based counterparts. Moreover, this study reveals great potential for CapsNet model in the field of HSI classification.

Abstract Introduction In this study, the effects of omega‐3 fatty acids were examined in a rat model of spinal cord injury. Methods The rats were classified into sham, control, spinal cord injury plus 50 mg/kg Omega‐3 fatty acids and spinal cord injury plus 100 mg/kg Omega‐3 fatty acids. The levels of oxidative, apoptotic, and inflammatory markers were examined in each of these groups. Results Altered lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (Gpx), and catalase were normalized. Omega‐3 fatty acid supplementation decreased tumor necrosis factor‐alpha (TNF‐α) and interleukin‐6 (IL‐6) levels by >50%. TNF‐α and IL‐6 mRNA expression were reduced. Caspase‐3, p53, bax, and pro‐NGF mRNA expression levels were increased by 1.3‐, 1.4‐, 1.2‐, and 0.9‐fold, respectively, whereas bcl‐2 mRNA expression was decreased by 0.77‐fold in control rats. Omega‐3 fatty acid supplementation decreased p53, caspase‐3, bax, and pro‐NGF mRNA expression by >40%, while the level of bcl‐2 mRNA expression was increased by 286.9%. Omega‐3 fatty acid supplementation decreased caspase‐3 and p53 protein expression by >30%. Conclusion Taken together, our results suggested that omega‐3 fatty acid supplementation reduced oxidative stress, apoptosis, and the levels of inflammatory markers in ischemia‐reperfusion‐induced rats.

Renal cell carcinoma (RCC) metastasis to the adrenal gland, perirenal adipose tissue, and ureter on the contralateral side is rare. We report a case of solitary metachronous clear cell renal cell carcinoma (ccRCC) metastasis to the contralateral retroperitoneal adipose tissue, which was identified after radical nephrectomy. A patient had undergone retroperitoneal laparoscopic radical nephrectomy for RCC in the right kidney in December 2012. Postoperative pathological analysis showed Fuhrman grade I ccRCC, T 1b N 0 M 0 . Three years after surgery, a solitary tumor of 1.0 × 1.0 cm was identified by an abdominal computed tomographic scan inside the retroperitoneal fat pad in front of the left posterior abdominal wall, without adhesion to the abdominal wall. The tumor was then completely resected by retroperitoneal laparoscopic resection. Pathological analysis showed that it was a metastasized lesion from a previous tumor. Nine months after surgery, there was no sign of recurrence confirmed by radiographic follow-up. Findings from this case indicate the unpredictability of dissemination of RCC. Our findings support a follow-up regimen that includes regular postoperative computed tomographic scans to identify early metastasis. To the best of our knowledge, this is the first reported case of contralateral retroperitoneal adipose metastasis after laparoscopic tumorectomy.

Wet joints are widely used in precast steel–concrete composite bridges to accelerate the construction of bridges, though a conventional wet joint usually has a poor ultimate shear capacity. To improve the shear capacity of the wet joint, a concave square frustum-shaped wet joint was proposed, and the failure modes and ultimate shear capacity were studied experimentally and numerically. Specimens with concave square frustum-shaped and conventional wet joints (labeled as S-SWJ and S-CWJ) were fabricated, and experiments were performed on them. The results showed that the ultimate shear capacity of S-SWJ was substantially enhanced compared to that of S-CWJ. To further explore the ultimate shear capacity of S-SWJ, the failure modes and the influences of concrete strength and shear key angle on the ultimate shear capacity were studied using a validated finite element (FE) model. Based on the FE analysis, the guidelines for obtaining a wet joint with desirable shear capacity are presented, which is useful for the design of wet joints with high ultimate shear capacity.

Abstract Background Enterocytozoon bieneusi is one of the most prevalent causative species of diarrhea and enteric diseases in various hosts. E. bieneusi has been identified in humans, mammals, birds, rodents and reptiles in China, but few studies have reported E. bieneusi in horses. Therefore, the present study was conducted to assess the prevalence, molecular characteristics and zoonotic potential of E. bieneusi among horses in southwestern China. Findings Three hundred and thirty-three fecal specimens were collected from horses on five farms in the Sichuan and Yunnan provinces of southwestern China. The prevalence of E. bieneusi was 22.5 % (75/333), as determined by nested polymerase chain reaction and sequencing analysis of the internal transcribed spacer region of the ribosomal RNA gene of E. bieneusi. Altogether, 10 genotypes were identified among the 75 E. bieneusi-positive samples: four of these genotypes were known (horse1, horse2, SC02 and D) and six were novel (SCH1-4 and YNH1-2). Multilocus sequence typing using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) revealed three, two, three and three genotypes at these four loci, respectively. In phylogenetic analysis, all the genotypes of E. bieneusi obtained in this study were clustered into three distinct groups: D, SC02 and SCH1-3 were clustered into group 1 (zoonotic potential); SCH4 was clustered into group 2 (cattle-hosted); whereas horse2, YNH1 and YNH2 were clustered into group 6 (unclear zoonotic potential). Conclusions This is the first report of E. bieneusi among horses in southwestern China. This is also the first multilocus genotyping analysis using microsatellite and minisatellite markers of E. bieneusi in horses. The presence of genotype D, which was previously identified in humans, and genotypes SC02 and SCH1-3, which belong to potential zoonotic group 1, these results indicate that horses are a potential source of human E. bieneusi infections in China.

Opening has an important impact on economic development. However, due to the fact that various regions have different economic structure and development level, there are differences in the size of its actual effect. Using extended Solow model and 2002 --2013 panel data among 13 major cities of Beijing, Tianjin, and Hebei, this paper analyzed the effects of Tianjin’s openness on economic development in Jing-Jin-Ji area. The result shows that improving the degree of Tianjin’s openness has a very significant impact on the joint development of Jing-Jin-Ji area and regional economy. Therefore, we must further promote structural reforms and improve Tianjin’s openness to give full play to its role in driving economic development in Jing-Jin-Ji area in the future.

The National Comprehensive Cancer Network recommends conservative follow-up for gastric gastrointestinal stromal tumors (GISTs) less than 2 cm. We have previously reported that the mitotic index of 22.22% of small gastric GISTs exceeded 5 per 50 high-power fields and recommended that all small gastric GISTs should be resected once diagnosed. The aim of the present study is to compare the safety and outcomes of endoscopic and open resection of small gastric GISTs. From May 2010 to March 2014, a total of 90 small gastric GIST patients were enrolled in the present study, including 40 patients who underwent surgical resection and 50 patients who underwent endoscopic resection. The clinicopathological characteristics, resection-related factors, and clinical outcomes were recorded and analyzed. The clinicopathological characteristics were comparable between the two groups except for tumor location and DOG-1 expression. Compared with the surgical resection group, the operation time was shorter (P = .000), blood loss was less (P = .000), pain intensity was lower (P < .05), duration of first flatus and defecation was shorter (P < .05), and medical cost of hospitalization was lower (P = .027) in the endoscopic resection group. The complications and postoperative hospital stay were comparable between the two groups. No in situ recurrence or liver metastasis was observed during follow-up. Endoscopic resection of small gastric GISTs is safe and feasible compared with surgical resection, although perforation could not be totally avoided during and after resection. The clinical outcome of endoscopic resection is also favorable.

Abstract Background Hyperlordosis is common in facioscapulohumeral muscular dystrophy (FSHD), which cannot be controlled by bracing. While the surgical treatment is neither reported nor recommended in previous studies, we report the first corrective surgery for hyperlordosis in one wheelchair-dependent FSHD patient. Case presentation A 15-year-old, wheelchair-dependent girl complaining of hyperlordosis and lower extremity weakness was diagnosed as FSHD. Preoperative examination showed hyperlordosis of 116° with scoliosis of 44°. Posterior correction and bone graft fusion was performed. Postoperatively, the hyperlordosis was corrected to 72° and better sitting posture was gotten. 12 months’ follow-up showed favorable correction with improvement in SF-36 and ODI score. Conclusions The correction for hyperlordosis in FSHD is controversial. We report the first successful case of operative treatment by corrective spine surgery in these rare and demanding patient collective. Corrective surgery for spinal deformity, such as hyperlordosis and scoliosis, could do some help in posture and improve the quality of life especially in wheelchair-dependent patients.

Convolutional neural networks provide an ideal solution for hyperspectral image (HSI) classification. However, the classification effect is not satisfactory when limited training samples are available. Focused on “small sample” hyperspectral classification, we proposed a novel 3D-2D-convolutional neural network (CNN) model named AD-HybridSN (Attention-Dense-HybridSN). In our proposed model, a dense block was used to reuse shallow features and aimed at better exploiting hierarchical spatial–spectral features. Subsequent depth separable convolutional layers were used to discriminate the spatial information. Further refinement of spatial–spectral features was realized by the channel attention method and spatial attention method, which were performed behind every 3D convolutional layer and every 2D convolutional layer, respectively. Experiment results indicate that our proposed model can learn more discriminative spatial–spectral features using very few training data. In Indian Pines, Salinas and the University of Pavia, AD-HybridSN obtain 97.02%, 99.59% and 98.32% overall accuracy using only 5%, 1% and 1% labeled data for training, respectively, which are far better than all the contrast models.

The deformation behavior and fracture mechanism of cast duplex stainless steel tubular specimens under different tensile stages were investigated through experimental and numerical evaluation. The results showed that the axial stress was redistributed due to the necking of the tubular specimen, the axial stress near the internal wall was larger than those near the external wall, and its maximum axial stress was distributed between the internal wall and the center of the wall thickness. Microcracks and voids were initiated under the maximum shear stress along the δ/γ phase interface and propagated to the ferrite interior. The voids were connected and merged into the main crack through the propagation of the microcracks. Moreover, the main crack first propagated to the internal wall and then rapidly propagated to the external wall. The fracture morphology can be divided into three types: shear lip zones that can be found on both the internal and external walls, and shear lip zones that can be found on either only the internal wall or the external wall.

The extracellular matrix (ECM) regulates cellular function through the dynamic biomechanical and biochemical interplay between the resident cells and their microenvironment. Pathologically stiff ECM promotes phenotype changes in hepatocytes during liver fibrosis. To investigate the effect of ECM stiffness on hepatocyte migration and function, we designed an easy fabricated polyvinyl alcohol (PVA) hydrogel in which stiffness can be controlled by changing the concentration of glutaraldehyde. Three stiffnesses of hydrogels corresponding to the health of liver tissue, early stage, and end stage of fibrosis were selected. These were 4.8 kPa (soft), 21 kPa (moderate), and 45 kPa (stiff). For hepatocytes attachment, the hydrogel was coated with fibronectin. To evaluate the optimal concentration of fibronectin, hydrogel was coated with 0.1 mg/mL, 0.01 mg/mL, 0.005 mg/mL, or 0.003 mg/mL fibronectin, and the migratory behavior of single hepatocyte cultured on different concentrations of fibronectin was analyzed. To further explore the effect of substrate stiffness on hepatocyte migration, we used a stiffness controllable commercial 3D collagen gel, which has similar substrate stiffness to that of PVA hydrogel. Our result confirmed the PVA hydrogel biocompatibility with high hepatocytes survival. Fibronectin (0.01 mg/mL) promoted optimal migratory behavior for single hepatocytes. However, for confluent hepatocytes, a stiff substrate promoted hepatocellular migration compared with the soft and moderate groups via enhancing the formation of actin- and tubulin-rich structures. The gene expression analysis and protein expression analysis showed that the stiff substrate altered the phenotype of hepatocytes and induced apoptosis. Hepatocytes in stiff 3D hydrogel showed a higher proportion of cell death and expression of filopodia.

Ordovician-Silurian subduction, Early Devonian arc-contient collision and followed post-collision extension are recorded in the north of the North China Craton. Most previous research has focused on the first two processes. Discussion on the post-collisional extension and its tetonic regime is still limited. In this study, 40Ar-39Ar muscovite ages obtained from the central part of the northern North China Craton were analyzed to shed light on the timing of post-collision extension. Garnet muscovite schist and muscovite quartz schist in the Jianshan Formation yielded 40Ar-39Ar muscovite plateau ages of 412 ± 3 Ma and 391 ± 3 Ma, respectively. Two other 40Ar-39Ar muscovite plateau ages (389 ± 2 Ma and 397 ± 2Ma) were obtained for two Mesoproterozoic monzogranites intruding into the Jianshan Formation. Based on previous research, the northern North China Craton underwent a collision event with Bainaimiao arc at c. 415 Ma, followed by post-collision extension in early Late Paleozoic. Therefore, combined with the newly acquired muscovite 40Ar-39Ar dating results, the Jianshan Formation might go through regional metamorphism at c. 412 Ma during the collision process. Subsequently, the Jianshan Formation and monzogranites intruding into it went through rapid exhumation along with metamorphism at c. 397–389 Ma in a post-collision extensional setting. The muscovite 40Ar-39Ar ages provide new markers for the exhumation history and the post-collisional extension setting during Early-Late Devonian in the study area. Furthermore, slab breakoff as the cause for this extensional setting is argued by the emplacement of the Early-Late Devonian alkaline rocks.

The characteristics of the transcritical CO2 heat pump water heater (HPWH) system are; a lower inlet hot water temperature (Ti-hw) (sometimes this is lower than the water source temperature), and an outlet gas cooler temperature (To-gc) which is affected by the Ti-hw and often lower than the critical temperature. In order to study the effects of the internal heat exchanger (IHX) on the operational performance of the transcritical CO2 HPWH when To-gc is low, a transcritical CO2 water source HPWH experiment platform is established to conduct experimental research and comparative analysis on the operational performance of the transcritical CO2 water source HPWH, with or without IHX. It is found that, if only the coefficient of performance (COP) and heating at the optimal exhaust pressure of the transcritical CO2 water source HPWH were considered, COP and the heating of the non-IHX system would be slightly higher than those of the IHX system at the lower hot water flow and water source temperature, and this increase was not obvious. At the higher hot water flow rate and water source temperature, COP and the heating of the non-IHX system were also higher than those of the IHX system, and the increase was obvious. The experiment results showed that, near the optimal exhaust pressure, the variation range of COP and heating of the IHX system is relatively small, and the system has a relatively high stability.

The present study was aimed at developing a low-cost but rapid technique for qualitative and quantitative detection of beef adulterated with pork. An electronic nose based on colorimetric sensors was proposed. The fresh beef rib steaks and streaky pork were purchased and used from the local agricultural market in Suzhou, China. The minced beef was mixed with pork ranging at levels from 0%~100% by weight at increments of 20%. Protein, fat, and ash content were measured for validation of the differences between the pure beef and pork used in basic chemical compositions. Fisher linear discriminant analysis (Fisher LDA) and extreme learning machine (ELM) were utilized comparatively for identification of the ground pure beef, beef−pork mixtures, and pure pork. Back propagation-artificial neural network (BP-ANN) models were built for prediction of the adulteration levels. Results revealed that the ELM model built was superior to the Fisher LDA model with higher identification rates of 91.27% and 87.5% in the training and prediction sets respectively. Regarding the adulteration level prediction, the correlation coefficient and the root mean square error were 0.85 and 0.147 respectively in the prediction set of the BP-ANN model built. This suggests, from all the results, that the low-cost electronic nose based on colorimetric sensors coupled with chemometrics has a great potential in rapid detection of beef adulterated with pork.

The pathogenesis of diabetes mellitus is a complicated process involving much gene regulation. The molecular mechanism of mulberry (Morus alba L.) leaf in the treatment of diabetes is not fully understood. In this study, we used the Illumina HiSeq™ 2,500 platform to explore the liver transcriptome of normal mice, STZ-induced diabetic mice, and mulberry leaf-treated diabetic mice, and we obtained 52,542,956, 52,626,414, and 52,780,196 clean reads, respectively. We identified differentially expressed genes (DEGs) during the pathogenesis of diabetes in mice. The functional properties of DEGs were characterized by comparison with the GO and KEGG databases, and the results show that DEGs are mainly involved in the metabolic pathway. qRT-PCR was used to analyse 27 differential genes involved in liver expression in different groups of diabetic mice. Among the DEGs, the expression of Scube1, Spns3, Ly6a, Igf2, and other genes between the control (C) and diabetic control (DC) groups was significantly upregulated; the expression of Grb10, Mup2, and Fasn was significantly downregulated; the expression of the Sqle, Lss, and Irs2 genes between the C group and diabetic group treated with mulberry (DD) was significantly upregulated; the expression of Fabp2, Ly6a, and Grb10 was significantly downregulated; and the expression of Sqle and Lss was significantly upregulated in the DC and DD groups, but Tap1, Igf2, and Spns3 were significantly downregulated. The results of Western blot validation showed that dynamic changes in proteins, such as IGF2, Ly6a, Grb10, and UBD, occurred to regulate the incidence of diabetes by influencing the insulin receptor substrate (IRS) signaling pathway.

With the idea of retrosynthetic analysis, which was raised in the 1960s, chemical synthesis analysis and pathway design have been transformed from a complex problem to a regular process of structural simplification. This review aims to summarize the developments of computer-assisted synthetic analysis and design in recent years, and how machine-learning algorithms contributed to them. LHASA system started the pioneering work of designing semi-empirical reaction modes in computers, with its following rule-based and network-searching work not only expanding the databases, but also building new approaches to indicating reaction rules. Programs like ARChem Route Designer replaced hand-coded reaction modes with automatically-extracted rules, and programs like Chematica changed traditional designing into network searching. Afterward, with the help of machine learning, two-step models which combine reaction rules and statistical methods became the main stream. Recently, fully data-driven learning methods using deep neural networks which even do not require any prior knowledge, were applied into this field. Up to now, however, these methods still cannot replace experienced human organic chemists due to their relatively low accuracies. Future new algorithms with the aid of powerful computational hardware will make this topic promising and with good prospects.

Nitrogen (N) is a major essential nutrient for plant growth, and rice is an important food crop globally. Although ammonium (NH4+) is the main N source for rice, nitrate (NO3-) is also absorbed and utilized. Rice responds to NO3- supply by changing root morphology. However, the mechanisms of rice root growth and formation under NO3- supply are unclear. Nitric oxide (NO) and auxin are important regulators of root growth and development under NO3- supply. How the interactions between NO and auxin in regulating root growth in response to NO3- are unknown. In this study, the levels of indole-3-acetic acid (IAA) and NO in roots, and the responses of lateral roots (LRs) and seminal roots (SRs) to NH4+ and NO3-, were investigated using wild-type (WT) rice, as well as osnia2 and ospin1b mutants. NO3- supply promoted LR formation and SR elongation. The effects of NO donor and NO inhibitor/scavenger supply on NO levels and the root morphology of WT and nia2 mutants under NH4+ or NO3- suggest that NO3--induced NO is generated by the nitrate reductase (NR) pathway rather than the NO synthase (NOS)-like pathway. IAA levels, [3H] IAA transport, and PIN gene expression in roots were enhanced under NO3- relative to NH4+ supply. These results suggest that NO3- regulates auxin transport in roots. Application of SNP under NH4+ supply, or of cPTIO under NO3- supply, resulted in auxin levels in roots similar to those under NO3- and NH4+ supply, respectively. Compared to WT, the roots of the ospin1b mutant had lower auxin levels, fewer LRs, and shorter SRs. Thus, NO affects root growth by regulating auxin transport in response to NO3-. Overall, our findings suggest that NO3- influences LR formation and SR elongation by regulating auxin transport via a mechanism involving NO.

Genetic improvements have significantly contributed to wheat production. Five wheat cultivars—widely grown in north China in the 1950s, 1990s, or 2010s—were grown in field experiments conducted in the 2014–2015 and 2015–2016 growing seasons. This study evaluated the genetic progress in wheat grain yield and its related traits in north China and explored how breeding and selection have influenced grain numbers and weights within spikelets in the past 60 years. The results showed that the significant increases in grain yield in the past 60 years were mainly due to increases in grain number per spike and grain weight, while spike number per m2 has not changed significantly. Improvements in thousand grain weight (TGW) from the 1950s to 2010s have occurred at four grain positions (G1 to G4). The relative contribution of G4 to TGW increased over time, but was much less than the contributions of G1, G2, and G3. Indeed, the average grain weight at G4 was much less than that of 1000 grains. The increase in grain number per spike since the 1950s was mainly due to an increase in grain number at G1, G2 and G3, with the relative contribution of grain position to grain number being G1 > G2 > G3 > G4. Dwarfing genes increased grain number per spike and grain number at G3 and G4, but not TGW. In future, yields could be boosted by enhancing grain weight at G4 and grain number at G3 and G4, while maintaining those at G1 and G2.