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Female soccer has seen a substantial rise in participation, as well as increased financial support from governing bodies over the last decade. Thus, there is an onus on researchers and medical departments to develop a better understanding of the physical characteristics and demands, and the health and performance needs of female soccer players. In this review, we discuss the current research, as well as the knowledge gaps, of six major topics: physical demands, talent identification, body composition, injury risk and prevention, health and nutrition. Data on female talent identification are scarce, and future studies need to elucidate the influence of relative age and maturation selection across age groups. Regarding the physical demands, more research is needed on the pattern of high-intensity sprinting during matches and the contribution of soccer-specific movements. Injuries are not uncommon in female soccer players, but targeting intrinsically modifiable factors with injury prevention programmes can reduce injury rates. The anthropometric and physical characteristics of female players are heterogeneous and setting specific targets should be discouraged in youth and sub-elite players. Menstrual cycle phase may influence performance and injury risk; however, there are few studies in soccer players. Nutrition plays a critical role in health and performance and ensuring adequate energy intake remains a priority. Despite recent progress, there is considerably less research in female than male soccer players. Many gaps in our understanding of how best to develop and manage the health and performance of female soccer players remain.

High-speed running and sprinting training play an important role in the development of physical capabilities, sport-specific performance and injury prevention among soccer players. This commentary aims to summarize the current evidence regarding high-speed running and sprinting training in professional soccer and to inform its implementation in research and applied settings. It is structured into four sections: 1) Evidence-based high-speed running and sprinting conditioning methodologies; 2) Monitoring of high-speed running and sprinting performance in soccer 3) Recommendations for effective implementation of high-speed running and sprinting training in applied soccer settings; 4) Limitations and future directions. The contemporary literature provides preliminary methodological guidelines for coaches and practitioners. The recommended methods to ensure high-speed running and sprinting exposure for both conditioning purposes and injury prevention strategies among soccer players are: high-intensity running training, field-based drills and ball-drills in the form of medium- and large-sided games. Global navigation satellite systems are valid and reliable technologies for high-speed running and sprinting monitoring practice. Future research is required to refine, and advance training practices aimed at optimizing individual high-speed running and sprinting training responses and associated long-term effects., Competing Interests: All other authors declare no competing interests. Authors confirm that this study meets the ethical standards of the journal 51., (Thieme. All rights reserved.)

Background: The assessment of biological maturity status plays an important role in talent identification and development programs., Aim: To compare age at predicted adult height and BAUS skeletal age as indicators of biological maturity status in youth soccer players using a construct-validity approach., Subjects and Methods: Participants were 114 players from the U12 to U17 age groups of a professional youth soccer academy. Maturity status was determined via percentage of predicted adult height based upon the Khamis-Roche method (somatic maturity) and assessed via the SonicBone BAUS TM system (skeletal maturity). Convergent and known-groups validity were evaluated between maturity assessment methods and by comparing maturity-related selection biases across age groups., Results: Although maturity status indicators were largely interrelated (r = .94, 95%CL 0.91-0.96), concordance (κ = 0.31 to 0.39) and Spearman's rank-order correlations (ρ = 0.45-0.52) of classification methods were moderate. A selection bias towards early maturing players emerged in the U14 age group which remained relatively consistent through to the U17 age group., Conclusions: Results confirm the construct-validity of both methods to assess biological maturity status although further validation relative to established indicators of biological maturity is needed. Furthermore, caution is also warranted when interpreting maturity status classification methods interchangeably given the poor concordance between classification methods.

The methylome and transcriptome signatures following exercise that are physiologically and metabolically relevant to sporting contexts such as team sports or health prescription scenarios (e.g., high intensity interval training/HIIT) has not been investigated. To explore this, we performed two different sport/exercise relevant high-intensity running protocols in five male sport team members using a repeated measures design of: (1) change of direction (COD) versus; (2) straight line (ST) running exercise with a wash-out period of at least 2 weeks between trials. Skeletal muscle biopsies collected from the vastus lateralis 30 min and 24 h post exercise, were assayed using 850K methylation arrays and a comparative analysis with recent (subject-unmatched) sprint and acute aerobic exercise meta-analysis transcriptomes was performed. Despite COD and ST exercise being matched for classically defined intensity measures (speed × distance and number of accelerations/decelerations), COD exercise elicited greater movement (GPS-Playerload), physiological (HR), metabolic (lactate) as well as central and peripheral (differential RPE) exertion measures compared with ST exercise, suggesting COD exercise evoked a higher exercise intensity. The exercise response alone across both conditions evoked extensive alterations in the methylome 30 min and 24 h post exercise, particularly in MAPK, AMPK and axon guidance pathways. COD evoked a considerably greater hypomethylated signature across the genome compared with ST exercise, particularly at 30 min post exercise, enriched in: Protein binding, MAPK, AMPK, insulin, and axon guidance pathways. Comparative methylome analysis with sprint running transcriptomes identified considerable overlap, with 49% of genes that were altered at the expression level also differentially methylated after COD exercise. After differential methylated region analysis, we observed that VEGFA and its downstream nuclear transcription factor, NR4A1 had enriched hypomethylation within their promoter regions. VEGFA and NR4A1 were also significantly upregulated in the sprint transcriptome and meta-analysis of exercise transcriptomes. We also confirmed increased gene expression of VEGFA , and considerably larger increases in the expression of canonical metabolic genes PPARGC1A (that encodes PGC1- α) and NR4A3 in COD vs. ST exercise. Overall, we demonstrate that increased physiological/metabolic load via COD exercise in human skeletal muscle evokes considerable epigenetic modifications that are associated with changes in expression of genes responsible for adaptation to exercise., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Maasar, Turner, Gorski, Seaborne, Strauss, Shepherd, Cocks, Pillon, Zierath, Hulton, Drust and Sharples.)

Purpose: The aim of the study was to examine the perceptions of training data feedback from key stakeholders within the coaching process of professional soccer clubs. A survey assessed the importance of training data towards reflection and decision-making, potential barriers and player preferences. Methods: A total of 176 participants comprising coaches, players and performance staff completed the survey. Results: The training data coaches most commonly identified as wanting to see to support reflection was 'high-intensity' actions and variables recognised by the coach as 'work rate/intensity'. All stake- holders reported training data as at least somewhat important in guiding their coaches' practices, with lack of a common goal and high volumes of information being the main barriers to effective feedback of training data. Players deemed feedback as positive to change their behaviour, with total distance, high-speed running and sprint distances as the information they would most like to see. It would be likely to be looked at via message or pinned up in the changing room. Conclusion: Training data are seen as an impactful and effective tool for use by all key stakeholders. Despite this, its use can be optimised by increasing opportunities for informal reflection, using less information, and improving communication of data.

Objective : The study aimed to compare the physiological characteristics and acute fatigue associated with position-specific speed endurance production (SEP) and maintenance (SEM) soccer drills. Methods : Twenty male soccer players performed a position specific drill consisting of 8 exercise bouts each lasting ~30 s interspersed by 150 s (SEP) and 60 s (SEM) of passive recovery. A selection of players (n = 10) completed neuromuscular assessments pre and post drill. Results : Players covered greater high speed (12%), very high speed (49%) and sprint (218%) running distances in SEP ( P  < 0.05, ES: 0.51-0.80). SEP resulted in greater peak (7%) and average (10%) running speeds ( P  < 0.01, ES: 0.70-0.93). Mean and peak heart rate responses were greater in SEM (4-10%, P  < 0.01, ES: 0.97-1.84) whilst blood lactate concentrations were higher following SEP (6%, P  < 0.05, ES: 0.42). Reductions in vertical countermovement jump height were more pronounced immediately after SEP (2%, P  < 0.05, ES: 0.36) but 24 h post SEM (4%, P  < 0.05, ES: 0.52). Horizontal countermovement jump performance was reduced immediately post SEP and SEM (3-5%, P  < 0.01, ES: 0.22-0.38) and 24 h post SEM (4%, ES: 0.32). Conclusion : The data demonstrate that position-specific SEP and SEM drills overload different physiological indices and induce small impairments in some neuromuscular measures.

The aim of this study was to evaluate the effect of acceleration on the external and internal load during repeated sprint exercises (RSE). This study used a cross-over design. Sixteen soccer players were included (mean ± SDs: age 21 ± 1 years; weight 71.1 ± 7.7 kg). RSE was 3 sets of 7 × 30 m sprints with 25 s and 3 min recovery between sprints and sets, respectively. RSE was performed using two protocols requiring either 10 m maximal acceleration (2.12 m . s -2 [RSE-MA]) or 10 m submaximal acceleration (1.66 m . s -2 [RSE-SA]). Global positioning systems (10 Hz; STATSports, Viper) were utilized to collect: high speed running (HSR), dynamic stress load (DSL), Heart Rate (HR) peak, time >85% HR peak, respiratory (RPEres) and muscular (RPEmus) rating of perceived exertion. RSE-MA induced higher load than RSE-SA in HSR (p = 0.037, ES = 0.20), DSL (p = 0.027, ES = 0.43), HR peak (p = 0.025, ES = 0.47), Time >85% HR peak (p = 0.028, ES = 1.11), RPEres (p = 0.001, ES = 1.10), and RPEmus (p = 0.001, ES = 0.73). This study shows that a different acceleration intensity in a RSE (MA vs. SA) impacts external and internal training load parameters.

Purpose: This study aimed to inform the energy requirements of highly trained adolescent soccer players. Total energy expenditure (TEE) was quantified in academy soccer players from the English Premier League (EPL)., Methods: Twenty-four male adolescent soccer players from an EPL academy (under (U) 12/13 (U12/13), n = 8; U15, n = 8; U18, n = 8) were assessed for baseline maturity (maturity offset), body composition (dual-energy x-ray absorptiometry), and resting metabolic rate (indirect calorimetry). Subsequently, TEE, energy intake (EI) and physical loading patterns were assessed over a 14-d in-season period using doubly labeled water, the remote food photographic method, and global positioning system technology, respectively., Results: U18 players presented with greater resting metabolic rate (2236 ± 93 kcal·d) and TEE (3586 ± 487 kcal·d; range, 2542-5172 kcal·d) than did both U15 (2023 ± 162 and 3029 ± 262 kcal·d, respectively; TEE range, 2738-3726 kcal·d) and U12/13 players (1892 ± 211 and 2859 ± 265 kcal·d, respectively; TEE range, 2275-3903 kcal·d; all, P < 0.01), although no difference in TEE was apparent between the U12/13 and U15 age groups. Fat-free mass was significantly different between all comparisons in a hierarchal manner (U18: 57.2 ± 6.1 kg > U15: 42.9 ± 5.8 kg > U12/13: 31.1 ± 3.5 kg; all, P < 0.01). Within age groups, no differences were apparent between EI and TEE (U12/13: -29 ± 277 kcal·d, P = 0.78; U15: -134 ± 327 kcal·d, P = 0.28; U18: -243 ± 724 kcal·d, P = 0.37), whereas U18 players (3180 ± 279 kcal·d) reported higher EI than did both U15 (2821 ± 338 kcal·d; P = 0.05) and U12/13 players (2659 ± 187 kcal·d; P < 0.01)., Conclusions: The TEE of male academy soccer players progressively increase as players progress through the academy age groups. In some individuals (evident in all age groups), TEE was greater than that previously observed in adult EPL soccer players.

ACL injuries are among the most severe knee injuries in elite sport, with a high injury burden and re-injury risk. Despite extensive literature on the injury and the higher incidence of injury and re-injury in female athletes, there is limited evidence on the return to sport (RTS) of elite female football players following ACL reconstruction (ACLR). RTS is best viewed on a continuum aligning the recovery and rehabilitation process with the ultimate aim - a return to performance (RTP erf ). We outline the RTS and RTP erf of an elite female football player following ACLR and her journey to the FIFA Women's World Cup, including the gym-based physical preparation and the on-pitch/sports-specific reconditioning. We used the 'control-chaos continuum' as a framework for RTS, guiding a return above pre-injury training load demands while considering the qualitative nature of movement in competition. We then implemented the 'RTP erf pathway' to facilitate a return to team training, competitive match play and a RTP erf . Objective information, clinical reasoning and shared decision-making contributed to this process and helped the player to reach her goal of representing her country at the FIFA Women's World Cup., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

To assess the real-life magnitude of the heading incidence in children's and youth' football in eight European countries with different "football cultures," a cross-sectional observational design, in which one match per team in 480 different teams from eight European countries (2017/18-2018/19), was recorded by video. One training session was recorded in 312 teams. Clubs with Under-10, Under-12 (female/male/mixed), and Under-16 female and male teams were eligible to participate. Heading frequencies and types were analyzed. Results are presented as headers per match/training and per team. Incidence rates (IR) per 1000 match/training hours were calculated. Under-10 teams carried out the lowest average number of headers per match (8.8), followed by Under-16 female (17.7), Under-12 (18.4), and Under-16 male (35.5). Total number of headers per match and team varied between countries. 80% of the total number of headers were single intentional headers, 12% heading duels, 3% unintentional headers by getting hit, and 5% others (trends apparent in all age groups). Three head injuries occurred during match play corresponding to an IR of 0.70 (95% CI, 0.23-2.16). The lowest number of headers per training and team was found in Under-10 (21.3), followed by Under-16 females (34.1), Under-12 (35.8), and Under-16 males (45.0). In conclusion, this large-scale study presents novel data about the number and type of headers in youth' football throughout Europe. A more precise understanding of the heading incidence, specifically in young players, is mandatory for the debate of restrictions on heading in youth football., (© 2020 The Authors. Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.)

Objectives: To identify the most common injury types/locations in high-level male youth soccer players (YSP)., Design: Prospective cohort surveillance study., Setting: Professional soccer club academies., Participants: Six hundred and twenty-four high-level male YSP [Under 9 (U9) to U23 year-old age groups] from academies in England, Spain, Uruguay and Brazil., Main Outcome Measures: Injury type, location and severity were recorded during one season. Injury severity was compared between age groups, while injury type and location were compared between nations., Results: Four hundred and forty-three training or match injuries were recorded, giving an injury rate of 0.71 per player. Non-contact injuries were most common (58.5%), with most (44.2%) resolved between 8 and 28 days. Most injuries (75.4%) occurred in the lower limbs, with muscle (29.6%) the most commonly injured tissue. U14 and U16 suffered a greater number of severe injuries relative to U12 and U19/U20/U23/Reserves. Tendon injury rate was higher in Brazil vs. Spain (p < 0.05), with low back/sacrum/pelvis injury rate highest in Spain (p < 0.05)., Conclusions: The proportion of severe injuries in U14 and U16 suggests YSP injury risk is maturation-dependent. Minimal differences in type and location between high-level YSP from four different countries suggest injury rates in this population are geographically similar., Competing Interests: Declaration of competing interest None declared., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

We investigated the association of multiple single nucleotide polymorphisms (SNPs) with athlete status and power/speed performance in elite male youth soccer players (ESP) and control participants (CON) at different stages of maturity. ESP (n = 535; aged 8-23 years) and CON (n = 151; aged 9-26 years) were genotyped for 10 SNPs and grouped according to years from predicted peak-height-velocity (PHV), i.e. pre- or post-PHV, to determine maturity status. Participants performed bilateral vertical countermovement jumps, bilateral horizontal-forward countermovement jumps, 20m sprints and modified 505-agility tests. Compared to CON, pre-PHV ESP demonstrated a higher ACTN3 (rs1815739) XX ('endurance') genotype frequency distribution, while post-PHV ESP revealed a higher frequency distribution of the PPARA (rs4253778) C-allele, AGT (rs699) GG genotype and NOS3 (rs2070744) T-allele ('power' genotypes/alleles). BDNF (rs6265) CC, COL5A1 (rs12722) CC and NOS3 TT homozygotes sprinted quicker than A-allele carriers, CT heterozygotes and CC homozygotes, respectively. COL2A1 (rs2070739) CC and AMPD1 (rs17602729) GG homozygotes sprinted faster than their respective minor allele carrier counterparts in CON and pre-PHV ESP, respectively. BDNF CC homozygotes jumped further than T-allele carriers, while ESP COL5A1 CC homozygotes jumped higher than TT homozygotes. To conclude, we have shown for the first time that pre- and post-PHV ESP have distinct genetic profiles, with pre-PHV ESP more suited for endurance, and post-PHV ESP for power and speed (the latter phenotypes being crucial attributes for post-PHV ESP). We have also demonstrated that power, acceleration and sprint performance were associated with five SNPs, both individually and in combination, possibly by influencing muscle size and neuromuscular activation., Competing Interests: The authors’ affiliations with commercial companies do not represent any competing interests and do not affect the authors’ roles stipulated in the ‘author contributions’ section of the manuscript. Further, this does not alter our adherence to PLOS ONE policies on sharing data and materials.

For the first time we aimed to: (1) assess fat-free mass (FFM) and RMR in youth soccer players, (2) compare measured RMR to estimated RMR using previously published prediction equations, and (3) develop a novel population-specific prediction equation. In a cross-sectional design, 99 males from a Premier League academy underwent assessments of body composition (DXA) and RMR (indirect-calorimetry). Measured RMR was compared to estimated values from five prediction equations. A novel RMR prediction equation was developed using stepwise multiple regression. FFM increased (P<0.05) between U12 (31.6±4.2 kg) and U16 (56.3±5.3 kg) after which no further increases occurred (P>0.05). RMR in the U12s (1655±195 kcal.day -1 ), U13s (1720±205 kcal.day -1 ) and U14s (1846±218kcal.day -1 ) was significantly lower than the U15s (1957±128 kcal.day -1 ), U16s (2042±155 kcal.day -1 ), U18s (1875±180 kcal.day -1 ) and U23s (1941±197 kcal.day -1 ) squads (P>0.05). FFM was the single best predictor of RMR (r 2 =0.43; P<0.01) and was subsequently included in the novel prediction equation: RMR (kcal.day -1 ) = 1315 + (11.1 x FFM in kg). Both FFM and RMR increase from 12-16 years old, thus highlighting the requirement to adjust daily energy intake to support growth and maturation. The novel prediction RMR equation developed may help to inform daily energy requirements.

Talent identification and development programmes have received broad attention in the last decades, yet evidence regarding the predictive utility of physical performance in female soccer players is limited. Using a retrospective design, we appraised the predictive value of performance-related measures in a sample of 228 youth female soccer players previously involved in residential Elite Performance Camps (age range: 12.7-15.3 years). With 10-m sprinting, 30-m sprinting, counter-movement jump height, and Yo-Yo Intermittent Recovery Test Level 1 (IR1) distance as primary predictor variables, the Akaike Information Criterion (AIC) assessed the relative quality of four penalised logistic regression models for determining future competitive international squads U17-U20 level selection. The model including Yo-Yo IR1 was the best for predicting career outcome. Predicted probabilities of future selection to the international squad increased with higher Yo-Yo IR1 distances, from 4.5% (95% confidence interval, 0.8 to 8.2%) for a distance lower than 440 m to 64.7% (95% confidence interval, 47.3 to 82.1%) for a score of 2040 m. The present study highlights the predictive utility of high-intensity endurance capacity for informing career progression in elite youth female soccer and provides reference values for staff involved in the talent development of elite youth female soccer players.

At the turn of the millennium, a review paper was published in this journal on talent identification and development in soccer (Williams & Reilly, 2000). In the current paper, we assess progress made in this field over the last twenty years relative to the areas for future research highlighted in the original review. We evaluate developments in light of the calls made by Williams and Reilly to: a) undertake more multidisciplinary rather than mono-disciplinary research; b) embrace longitudinal rather than cross-sectional research designs; c) expand the research base on female football; and, d) better identify the subjective criteria used by scouts when selecting one player over another for entry into a formalised training environment. The body of mono-disciplinary research on this topic continues to expand, and progress has been made in publishing multidisciplinary, prospective, longitudinal data sets, along with advanced statistical modelling procedures, as well as in identifying the experiential criteria used by scouts. We found some variables in these studies have predictive value from adolescence to adult performance level in soccer. We present suggestions for future research to enhance knowledge and understanding of the best practices underpinning the identification and development of future generations of professional players.

We aimed to assess the coach-player agreement of subjective soccer training loads via differential ratings of perceived exertion (dRPE). The coach initially underwent quantifiable familiarisation (blackness test) with the Borg CR100 scale. Data were collected from 16 semi-professional soccer players across seven consecutive training sessions. For the measurement of subjective training load, the coach and players provided dRPE (CR100) for legs (RPE-L), breathlessness (RPE-B) and technical exertion (RPE-T). Coach-prescribed dRPE were recorded prior to training, with coach observed and player reported dRPE collected post training. Statistical equivalence bounds for agreement between coach (prescribed and observed) and player reported dRPE scores were 4 arbitrary units on the CR100 and we used a probability outcome of likely (≥75%) to infer realistic equivalence. Following three familiarisation sessions, the coach improved their blackness test score from 39% to 83%. Coach observed and player reported RPE-T scores were likely equivalent, with all other comparisons not realistically equivalent. Since training prescription is coach-led, our data highlight the importance of accurate internal load measurement and feedback in soccer. The improved accuracy and precision of coach intensity estimation after three attempts at the blackness test suggests that this method could be worthwhile to researchers and practitioners employing dRPE.

Maintaining muscle mass and function during rehabilitation from anterior cruciate ligament injury is complicated by the challenge of accurately prescribing daily energy intakes aligned to energy expenditure. Accordingly, we present a 38-week case study characterizing whole body and regional rates of muscle atrophy and hypertrophy (as inferred by assessments of fat-free mass from dual-energy X-ray absorptiometry) in a professional male soccer player from the English Premier League. In addition, in Week 6, we also quantified energy intake (via the remote food photographic method) and energy expenditure using the doubly labeled water method. Mean daily energy intake (CHO: 1.9-3.2, protein: 1.7-3.3, and fat: 1.4-2.7 g/kg) and energy expenditure were 2,765 ± 474 and 3,178 kcal/day, respectively. In accordance with an apparent energy deficit, total body mass decreased by 1.9 kg during Weeks 1-6 where fat-free mass loss in the injured and noninjured limb was 0.9 and 0.6 kg, respectively, yet, trunk fat-free mass increased by 0.7 kg. In Weeks 7-28, the athlete was advised to increase daily CHO intake (4-6 g/kg) to facilitate an increased daily energy intake. Throughout this period, total body mass increased by 3.6 kg (attributable to a 2.9 and 0.7 kg increase in fat free and fat mass, respectively). Our data suggest it may be advantageous to avoid excessive reductions in energy intake during the initial 6-8 weeks post anterior cruciate ligament surgery so as to limit muscle atrophy.

Purpose: The powerful activity profile of elite soccer match play has not been documented appropriately to inform specific maximal power assessment and development criteria. The aims of the current study were to develop a reliable  soccer-specific powerful action (SSPA) notational analysis coding system that could be used to compare frequency and durations of powerful actions during elite youth soccer match play., Methods: Sixteen elite male English Premier League (EPL) Academy players (19 ± 1 yrs) were recorded by an individual camera during 16 competitive EPL U18 and U21 games. Video footage was analyzed using performance analysis software and SSPAs were coded according to the following categories: initial acceleration, leading acceleration, sprint, unilateral jump and bilateral jump., Results: The SSPA coding system demonstrated very good inter- and intra-rater reliability (kappa coefficients ≥ 0.827). Elite youth EPL soccer players undertook significantly more initial (31 ± 9) and leading (37 ± 12) accelerations than sprints (8 ± 3; p = .014, d = 1.7, and p < .001, d = 1.7, respectively) and jumps (6 ± 5; p = .002, d = 1.7 and p < .001, d = 1.7, respectively). Players performed a significantly greater number of initial and leading accelerations with action durations below 1.5 s compared to above 1.5 s (p = .001, d = 1.6, and p = .002, d = 1.4), respectively., Conclusions: Our SSPA coding system provides a reliable observational instrument for quantifying the frequency and duration of powerful actions performed during elite soccer match play. In our sample of elite youth soccer players, horizontal accelerations of short duration (< 1.5 s) from different starting speeds appear the most dominant powerful action in elite youth soccer match play.

The current study examined how sleep may be influenced by the scheduling of training and match load within 10 youth-soccer players. Sleep was measured over a 14-day in-season period using a commercially available wireless sleep monitor. Each collected sleep variable; lights out, sleep latency, total sleep time wake after sleep onset and final awakening, was compared for the specific day within the training schedule (e.g. match day [MD], day after match [MD + 1]) and to training/match load (high-speed distance (>5.5 m/s) [HSD] and rating of perceived exertion. The data were analysed using mixed models and effect sizes, to describe the magnitude of effects that training schedule and training load may have on sleep. A reduction of sleep duration was observed on the day after the match (MD + 1) in relation to the training days preceding the match (MD-2: -65 min, ES: 0.89 ± 0.79; MD-1 -61 min, ES: 0.82 ± 0.64) and reduction on match day (+45 min; ES: 1.91 ± 1.69). This may suggest youth-soccer players actively change their sleep scheduling behaviours in relation to the imposed soccer schedule. Increased high-speed running (for every 100 m) showed a small increase to total sleep time (+9 min; ES: 0.48 ± 0.31). This may suggest that increases in training load may be associated with small increases in sleep quantity. Such observations may highlight that the type of day and the associated load within the training microcycle may have important consequences for sleep within youth-soccer players.

We assessed the effects of post-exercise cold-water immersion (CWI) in modulating PGC-1α mRNA expression in response to exercise commenced with low muscle glycogen availability. In a randomized repeated-measures design, nine recreationally active males completed an acute two-legged high-intensity cycling protocol (8 × 5 min at 82.5% peak power output) followed by 10 min of two-legged post-exercise CWI (8°C) or control conditions (CON). During each trial, one limb commenced exercise with low (LOW: <300 mmol·kg -1 dw) or very low (VLOW: <150 mmol·kg -1 dw) pre-exercise glycogen concentration, achieved via completion of a one-legged glycogen depletion protocol undertaken the evening prior. Exercise increased (P < 0.05) PGC-1α mRNA at 3 h post-exercise. Very low muscle glycogen attenuated the increase in PGC-1α mRNA expression compared with the LOW limbs in both the control (CON VLOW ~3.6-fold vs. CON LOW ~5.6-fold: P = 0.023, ES 1.22 Large) and CWI conditions (CWI VLOW ~2.4-fold vs. CWI LOW ~8.0 fold: P = 0.019, ES 1.43 Large). Furthermore, PGC-1α mRNA expression in the CWI-LOW trial was not significantly different to the CON LOW limb (P = 0.281, ES 0.67 Moderate). Data demonstrate that the previously reported effects of post-exercise CWI on PGC-1α mRNA expression (as regulated systemically via β-adrenergic mediated cell signaling) are offset in those conditions in which local stressors (i.e., high-intensity exercise and low muscle glycogen availability) have already sufficiently activated the AMPK-PGC-1α signaling axis. Additionally, data suggest that commencing exercise with very low muscle glycogen availability attenuates PGC-1α signaling., (© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)

Purpose : To better understand the energy and carbohydrate (CHO) requirements of a professional goalkeeper (GK) in elite soccer, the authors quantified physical loading, energy expenditure (EE), and energy intake (EI) during a 2-games-per-week in-season microcycle. Methods : Daily training and match loads were assessed in a professional GK (age 26 y, height 191 cm, body mass 85.6 kg) from the English Premier League using global positioning systems (GPS) and ProZone®, respectively. Assessments of EE (using the doubly labeled water method) and EI (using food diaries supported by the remote food photographic method and 24-h recalls) were also completed. Results : Physical loading was greater on match days than training days as inferred from total distance (4574 [432] vs 1959 [500] m), average speed (48 [5] vs 40 [4] m/min), and distance completed when jogging (993 [194] vs 645 [81] m) and running (138 [16] vs 21 [20] m). Average daily energy and macronutrient intake appear reflective of a self-selected "low-CHO" diet (energy 3160 [381] kcal, CHO 2.6 [0.6], protein 2.4 [0.4], fat 1.9 [0.3] g/kg body mass). Mean daily EE was 2894 kcal. Conclusions : The average daily EE of this professional GK was approximately 600 kcal/d lower than that previously reported in outfield players from the same team. Such data suggest that the nutritional requirements of a GK should be carefully considered depending on the required daily and weekly loading patterns.

Background: Monitoring the external ground reaction forces (GRF) acting on the human body during running could help to understand how external loads influence tissue adaptation over time. Although mass-spring-damper (MSD) models have the potential to simulate the complex multi-segmental mechanics of the human body and predict GRF, these models currently require input from measured GRF limiting their application in field settings. Based on the hypothesis that the acceleration of the MSD-model's upper mass primarily represents the acceleration of the trunk segment, this paper explored the feasibility of using measured trunk accelerometry to estimate the MSD-model parameters required to predict resultant GRF during running., Methods: Twenty male athletes ran at approach speeds between 2-5 m s -1 . Resultant trunk accelerometry was used as a surrogate of the MSD-model upper mass acceleration to estimate the MSD-model parameters (ACC param ) required to predict resultant GRF. A purpose-built gradient descent optimisation routine was used where the MSD-model's upper mass acceleration was fitted to the measured trunk accelerometer signal. Root mean squared errors (RMSE) were calculated to evaluate the accuracy of the trunk accelerometry fitting and GRF predictions. In addition, MSD-model parameters were estimated from fitting measured resultant GRF (GRF param ), to explore the difference between ACC param and GRF param ., Results: Despite a good match between the measured trunk accelerometry and the MSD-model's upper mass acceleration (median RMSE between 0.16 and 0.22 g), poor GRF predictions (median RMSE between 6.68 and 12.77 N kg -1 ) were observed. In contrast, the MSD-model was able to replicate the measured GRF with high accuracy (median RMSE between 0.45 and 0.59 N kg -1 ) across running speeds from GRF param . The ACC param from measured trunk accelerometry under- or overestimated the GRF param obtained from measured GRF, and generally demonstrated larger within parameter variations., Discussion: Despite the potential of obtaining a close fit between the MSD-model's upper mass acceleration and the measured trunk accelerometry, the ACC param estimated from this process were inadequate to predict resultant GRF waveforms during slow to moderate speed running. We therefore conclude that trunk-mounted accelerometry alone is inappropriate as input for the MSD-model to predict meaningful GRF waveforms. Further investigations are needed to continue to explore the feasibility of using body-worn micro sensor technology to drive simple human body models that would allow practitioners and researchers to estimate and monitor GRF waveforms in field settings., Competing Interests: The authors declare there are no competing interests.

We investigated whether single nucleotide polymorphisms (SNPs) within genes encoding the alpha-1 chain of type I ( COL1A1, rs2249492 ; rs1800012 ), type II ( COL2A1, rs2070739 ), and type V (COL5A1, rs12722 ) collagen were associated with the variable response to exercise-induced muscle damage (EIMD). Knee extensor muscle strength and soreness were assessed pre-, post-, and 48 h post-EIMD (120 maximal eccentric knee extensor contractions) in 65 young healthy participants, who were genotyped for the aforementioned SNPs. We found that COL1A1 (minor) T-allele carriers ( rs1800012 ) and (major) T-allele homozygotes ( rs2249492 ) were generally weaker ( P ≤ 0.019); and (minor) A-allele carriers of COL2A1 ( P = 0.002) and (major) T-allele carriers of COL5A1 ( P = 0.004) SNPs reported greater muscle soreness, all compared with their respective major ( rs1800012 ; rs2070739 ) and minor ( rs2249492 ; rs12722 ) allele homozygote counterparts. To conclude, the risk alleles of these four SNPs appear to negatively influence muscle strength and post-EIMD recovery, possibly via a dysregulated collagen network affecting the muscle's mechanical properties.

Bridge, CA, Sparks, SA, McNaughton, LR, Close, GL, Hausen, M, Gurgel, J, and Drust, B. Repeated exposure to taekwondo combat modulates the physiological and hormonal responses to subsequent bouts and recovery periods. J Strength Cond Res 32(9): 2529-2541, 2018-This study examined the physiological and hormonal responses to successive taekwondo combats using an ecologically valid competition time structure. Ten elite male international taekwondo competitors (age 19 ± 3 years) took part in a simulated championship event. The competitors performed 4 combats that were interspersed with different recovery intervals (63 ± 4, 31 ± 3 and 156 ± 5 minutes, respectively). Heart rate (HR) was measured during the combats and venous blood samples were obtained both before and after each combat to determine the plasma metabolite and hormone concentrations. The plasma noradrenaline (21.8 ± 12.8 vs. 15.0 ± 7.0 nmol·l) and lactate (13.9 ± 4.2 vs. 10.5 ± 3.2 mmol·l) responses were attenuated (p < 0.05) between combat 1 and 4. Higher (p < 0.05) HR responses were evident in the final combat when compared with the earlier combats. Higher (p < 0.05) resting HR (139 ± 10 vs. 127 ± 12 b·min), plasma lactate (3.1 ± 1.2 vs. 2.0 ± 0.7 mmol·l), glycerol (131 ± 83 vs. 56 ± 38 μmol·l) and nonesterified free fatty acid (0.95 ± 0.29 vs. 0.71 ± 0.28 mmol·l) concentrations were measured before combat 3 compared with combat 1. Repeated exposure to taekwondo combat using an ecologically valid time structure modulates the physiological and hormonal responses to subsequent bouts and recovery periods. Strategies designed to assist competitors to effectively manage the metabolic changes associated with the fight schedule and promote recovery between the bouts may be important during championship events.

Purpose: To investigate potential differences in patellar tendon properties between elite and non-elite soccer players, and to establish whether tendon properties were related to power assessed during unilateral jumps performed in different directions., Methods: Elite (n = 16; age 18.1 ± 1.0 years) and non-elite (n = 13; age 22.3 ± 2.7 years) soccer players performed vertical, horizontal-forward and medial unilateral countermovement jumps (CMJs) on a force plate. Patellar tendon (PT) cross-sectional area, elongation, strain, stiffness, and Young's modulus (measured at the highest common force interval) were assessed with ultrasonography and isokinetic dynamometry., Results: Elite demonstrated greater PT elongation (6.83 ± 1.87 vs. 4.92 ± 1.88 mm, P = 0.011) and strain (11.73 ± 3.25 vs. 8.38 ± 3.06%, P = 0.009) than non-elite soccer players. Projectile range and peak horizontal power during horizontal-forward CMJ correlated positively with tendon elongation (r = 0.657 and 0.693, P < 0.001) but inversely with Young's modulus (r = - 0.376 and - 0.402; P = 0.044 and 0.031). Peak medial power during medial CMJ correlated positively with tendon elongation (r = 0.658, P < 0.001) but inversely with tendon stiffness (r = - 0.368, P = 0.050)., Conclusions: Not only does a more compliant patellar tendon appear to be an indicator of elite soccer playing status but it may also facilitate unilateral horizontal-forward and medial, but not vertical CMJ performance. These findings should be considered when prescribing talent selection and development protocols related to direction-specific power in elite soccer players.

Ward, PA, Ramsden, S, Coutts, AJ, Hulton, AT, and Drust, B. Positional differences in running and nonrunning activities during elite American football training. J Strength Cond Res XX(X): 000-000, 2017-The aim of this investigation was to describe differences in training loads between position groups within professional American football. Integrated micro technology data were collected on 63 NFL football players during an American football training camp. Five key metrics (total distance, high-speed distance, player load (PL), PL per minute, and total inertial movement analysis [IMA]) served to quantify both running and nonrunning activities. Players were classified into position groups (defensive back [DB], defensive linemen [DL], linebacker, offensive linemen [OL], quarterback, running back, tight end, and wide receiver [WR]). Training sessions were identified by their relationship to the upcoming match (e.g., -4, -3, and -2). Running and nonrunning activities varied between position groups relative to the training day. Differences in total distance between DB and WR were observed to be unclear across the 3 training days (game day [GD] -4: 74 ± 392 m; GD -3: -122 ± 348 m; and GD -2: -222 ± 371 m). However, moderate to large differences were observed between these 2 positions and the other positional groups. A similar relationship was observed in PL and PL per minute, with the DB and WR groups performing greater amounts of load compared with other positional groups. Differences in high-speed distance varied across positional groups, indicating different outputs based on ergonomic demands. The OL and DL groups ran less but engaged in a higher amount of nonrunning activities (total IMA) with differences ranging from moderate to large across the 3 training days. Total IMA differences between offensive and defensive linemen were unclear on GD -4 (-4 ± 9) and GD -2 (-2 ± 8) and likely moderate on GD -3 (-9 ± 9). Positional differences with regard to running and nonrunning activities highlight the existence of position-specific training within a training microcycle. In addition, total IMA provides a useful metric for quantifying sport-specific movements within the game of American football.

Purpose: To investigate differences in neuromuscular factors between elite and nonelite players and to establish which factors underpin direction-specific unilateral jump performance., Methods: Elite (n = 23; age, 18.1 [1.0] y; body mass index, 23.1 [1.8] kg·m -2 ) and nonelite (n = 20; age, 22.3 [2.7] y; body mass index, 23.8 [1.8] kg·m -2 ) soccer players performed 3 unilateral countermovement jumps (CMJs) on a force platform in the vertical, horizontal-forward, and medial directions. Knee extension isometric maximum voluntary contraction torque was assessed using isokinetic dynamometry. Vastus lateralis fascicle length, angle of pennation, quadriceps femoris muscle volume (M vol ), and physiological cross-sectional area (PCSA) were assessed using ultrasonography. Vastus lateralis activation was assessed using electromyography., Results: Elite soccer players presented greater knee extensor isometric maximum voluntary contraction torque (365.7 [66.6] vs 320.1 [62.6] N·m; P = .045), M vol (2853 [508] vs 2429 [232] cm 3 ; P = .001), and PCSA (227 [42] vs 193 [25] cm 2 ; P = .003) than nonelite. In both cohorts, unilateral vertical and unilateral medial CMJ performance correlated with M vol and PCSA (r ≥ .310, P ≤ .043). In elite soccer players, unilateral vertical and unilateral medial CMJ performance correlated with upward phase vastus lateralis activation and angle of pennation (r ≥ .478, P ≤ .028). Unilateral horizontal-forward CMJ peak vertical power did not correlate with any measure of muscle size or activation but correlated inversely with angle of pennation (r = -.413, P = .037)., Conclusions: While larger and stronger quadriceps differentiated elite from nonelite players, relationships between neuromuscular factors and unilateral jump performance were shown to be direction-specific. These findings support a notion that improving direction-specific muscular power in soccer requires improving a distinct neuromuscular profile.

Background: There are no data describing the acute hormonal responses to concurrent-training programmes in youth elite soccer players. Therefore, the aim of this study was to describe the total testosterone (T), cortisol (C), and growth hormone (hGH) responses during two same-day concurrent-training (CT) trials in elite soccer players., Methods: Thirteen youth elite players (age: 17.0±0.2 years; height, 1.80±0.07 m; body mass, 73.1±5.7 kg; V̇O2max, 64.4±4.8 mL-1.kg-1.min-1) from an English premier league soccer club completed two CT trials. "Trial 1" (CT1); E (10.30 a.m.) followed by S (02.00 p.m.) and Trial 2 (CT2); strength-training (S) 09.00 a.m. followed by a soccer-specific endurance-training session (E) at 10.30 a.m. Venous blood samples were collected at 5 time-points around training and food intake (T1; 08.00 a.m., T2; 09.45h, T3; 12.30h, T4; 01.45 p.m. and T5; 03.15 p.m.) and analyzed for T (nmol/L) and C (nmol/L) and hGH (ug/L)., Results: There was no main effects found between exercise conditions for any hormones (T; P=0.22, C; P=0.07, hGH; P=0.21). Effect size analysis revealed a moderate effect for T at T3 (ES=0.63, CT1; 18.4±3.8, CT2; 15.7±4.7 nmol/L-1). A moderate effect for T Area Under the Curve (AUC) was observed between conditions (CT1; 300±76 versus CT2; 244±81 [AU]; ES=0.71). A moderate effect was apparent for C concentrations T4 in (ES=-0.95, CT1; 230±69, CT2; 314±105 nmol/L-1). Moderate effect sizes were observed at T3 and T4 (ES=0.82, CT1; 1.28±1.17, CT2; 0.47±0.75, ES=0.72, CT1; 0.11±0.05, CT2; 0.07±0.06 ug/L-1 respectively). A moderate effect for hGH AUC was observed between trials (CT1; 14±11 versus CT2; 5±9; [AU], ES=-1.08)., Conclusions: The organisation of the concurrent-training protocols used in this study has a negligible impact upon the acute T, C and hGH in youth elite soccer players.

Purpose: Competitive match play is a dominant component of the physical load completed by soccer players in a training microcycle. Characterizing the temporal disruption in homeostasis that follows exercise may provide some insight on the potential for match play to elicit an adaptive response., Methods: Countermovement-jump (CMJ) performance was characterized 3 d postmatch for 15 outfield players from an English Premier League soccer team (age 25.8 ± 4.1 y, stature 1.78 ± 0.08 m, body mass: 71.7 ± 9.1 kg) across a season. These players were classified as either starters (n = 9) or nonstarters (n = 6), according to their average individual playing time (more or less than 60 min/match). Linear mixed models were used to investigate the influence of indicators of match activity (total distance covered [TD] and high-intensity running distance [HI]) on CMJ height and peak power (PP)., Results: Starting players covered much greater TD (ES = 1.5) and HI (ES = 1.4) than nonstarters. Furthermore, there was a possible positive effect of HI on CMJ height and PP. This relationship suggests that an additional 0.6 km of HI would increase CMJ height and PP by slightly more than the smallest-worthwhile-change values of 0.6 cm and 1.0 W/kg, respectively. This small yet practically relevant increase in performance may suggest that match play, more specifically the intense activities that are associated with the match, provides a physiological stimulus for neuromuscular adaptation., Conclusions: These data may have implications for the preparation of soccer squads, especially the training requirements of starting and nonstarting players.

The purpose of this study was to investigate the influence that different frequencies of deceleration and acceleration actions had on the physiological demands in professional soccer players. Thirteen players were monitored via microelectromechanical devices during shuttle running protocols which involved one, three, or seven 180 degree directional changes. Heart rate exertion (HRE) (1.1 ± 0.7) and rating of perceived exertion (RPE) (5 ± 1) were significantly higher for the protocol which included seven directional changes when compared to the protocols which included one (HRE 0.5 ± 0.3, ES = 1.1, RPE 3 ± 0, ES = 2.7) or three (HRE 0.5 ± 0.2, ES = 1.1, RPE 3 ± 1, ES = 1.9) directional changes ( p < 0.05). The gravitational force (g-force) as measured through accelerometry (ACC) also showed a similar trend when comparing the seven (8628.2 ± 1630.4 g) to the one (5888.6 ± 1159.1 g, ES = 1.9) or three (6526.9 ± 1257.6 g, ES = 1.4) directional change protocols ( p < 0.05). The results of this study suggest that increasing the frequency of decelerations and accelerations at a high intensity running (HIR) speed alters the movement demands and elevates the physiological responses in professional players. This data has implications for the monitoring of physical performance and implementation of training drills.

Beato, M, Bianchi, M, Coratella, G, Merlini, M, and Drust, B. Effects of plyometric and directional training on speed and jump performance in elite youth soccer players. J Strength Cond Res 32(2): 289-296, 2018-Soccer players perform approximately 1,350 activities (every 4-6 seconds), such as accelerations/decelerations and changes of direction (CODs) during matches. It is well established that COD and plyometric training have a positive impact on fitness parameters in football players. This study analyzed the effect of a complex COD and plyometric protocol (CODJ-G) compared with an isolated COD protocol (COD-G) training on elite football players. A randomized pre-post parallel group trial was used in this study. Twenty-one youth players were enrolled in this study (mean ± SD; age 17 ± 0.8 years, mass 70.1 ± 6.4 kg, and height 177.4 ± 6.2 cm). Players were randomized into 2 different groups: CODJ-G (n = 11) and COD-G (n = 10), training frequency of 2 times a week more than 6 weeks. Sprint 10, 30, and 40 m, long jump, triple hop jump, and 505 COD test were considered. Exercise-induced within-group changes in performance for both CODJ-G and COD-G: long jump (effect size [ES] = 0.32 and ES = 0.26, respectively) and sprint 10 m (ES = -0.51 and ES = -0.22, respectively), after 6 weeks of training. Moreover, CODJ-G reported substantially better results (between-group changes) in long jump test (ES = 0.32). In conclusion, this study showed that short-term protocols (CODJ-G and COD-G) are important and able to give meaningful improvements on power and speed parameters in a specific soccer population. CODJ-G showed a larger effect in sprint and jump parameters compared with COD-G after the training protocol. This study offers important implications for designing COD and jumps training in elite soccer.

Murtagh, CF, Brownlee, TE, O'Boyle, A, Morgans, R, Drust, B, and Erskine, RM. Importance of speed and power in elite youth soccer depends on maturation status. J Strength Cond Res 32(2): 297-303, 2018-Maturation status is a confounding factor when identifying talent in elite youth soccer players (ESP). By comparing performance of ESP and control participants (CON) matched for maturation status, the aims of our study were to establish the importance of acceleration, sprint, horizontal-forward jump, and vertical jump capabilities for determining elite soccer playing status at different stages of maturation. Elite youth soccer players (n = 213; age, 14.0 ± 3.5 years) and CON (n = 113; age, 15.0 ± 4.4 years) were grouped using years from/to predicted peak height velocity (PHV) to determine maturation status (ESP: pre-PHV, n = 100; mid-PHV, n = 25; post-PHV, n = 88; CON: pre-PHV, n = 44; mid-PHV, n = 15; post-PHV, n = 54). Participants performed 3 reps of 10- and 20-m sprint, bilateral vertical countermovement jump (BV CMJ), and bilateral horizontal-forward CMJ (BH CMJ). Elite youth soccer players demonstrated faster 10-m (p < 0.001) and 20-m sprint (p < 0.001) performance than CON at all stages of maturation. Mid-PHV and post-PHV ESP achieved greater BV CMJ height (p < 0.001) and BH CMJ distance (ESP vs. CON; mid-PHV: 164.32 ± 12.75 vs. 136.53 ± 21.96 cm; post-PHV: 197.57 ± 17.05 vs. 168.06 ± 18.50 cm; p < 0.001) compared with CON, but there was no difference in BV or BH CMJ between pre-PHV ESP and CON. Although 10 and 20 m and sprint performance may be determinants of elite soccer playing status at all stages of maturation, horizontal-forward and vertical jumping capabilities only discriminate ESP from CON participants at mid- and post-PHV. Our data therefore suggest that soccer talent identification protocols should include sprint, but not jump assessments in pre-PHV players.

The daily distribution of macronutrient intake can modulate aspects of training adaptations, performance and recovery. We therefore assessed the daily distribution of macronutrient intake (as assessed using food diaries supported by the remote food photographic method and 24-hr recalls) of professional soccer players (n = 6) of the English Premier League during a 7-day period consisting of two match days and five training days. On match days, average carbohydrate (CHO) content of the prematch (<1.5 g·kg -1 body mass) and postmatch (1 g·kg -1 body mass) meals (in recovery from an evening kick-off) were similar (p > .05) though such intakes were lower than contemporary guidelines considered optimal for prematch CHO intake and postmatch recovery. On training days, we observed a skewed and hierarchical approach (p < .05 for all comparisons) to protein feeding such that dinner (0.8 g·kg -1 )>lunch (0.6 g·kg -1 )>breakfast (0.3 g·kg -1 )>evening snacks (0.1 g·kg -1 ). We conclude players may benefit from consuming greater amounts of CHO in both the prematch and postmatch meals so as to increase CHO availability and maximize rates of muscle glycogen resynthesis, respectively. Furthermore, attention should also be given to ensuring even daily distribution of protein intake so as to potentially promote components of training adaptation.

Objectives: We aimed to determine whether countermovement jumps (CMJs; unilateral and bilateral) performed in different directions assessed independent lower-limb power qualities, and if unilateral CMJs would better differentiate between elite and non-elite soccer players than the bilateral vertical (BV) CMJ., Design: Elite (n=23; age, 18.1±1.0years) and non-elite (n=20; age, 22.3±2.7years) soccer players performed three BV, unilateral vertical (UV), unilateral horizontal-forward (UH) and unilateral medial (UM) CMJs., Methods: Jump performance (height and projectile range), kinetic and kinematic variables from ground reaction forces, and peak activation levels of the vastus lateralis and biceps femoris (BF) muscles from surface electromyography, were compared between jumps and groups of players., Results: Peak vertical power (V-power) was greater in BV (220.2±30.1W/kg) compared to UV (144.1±16.2W/kg), which was greater than UH (86.7±18.3W/kg) and UM (85.5±13.5W/kg) (all, p<0.05) but there was no difference between UH and UM (p=1.000). Peak BF EMG was greater in UH compared to all other CMJs (p≤0.001). V-power was greater in elite than non-elite for all CMJs (p≤0.032) except for BV (p=0.197). Elite achieved greater UH projectile range than non-elite (51.6±15.4 vs. 40.4±10.4cm, p=0.009)., Conclusions: We have shown that UH, UV and UM CMJs assess distinct lower-limb muscular power capabilities in soccer players. Furthermore, as elite players outperformed non-elite players during unilateral but not BV CMJs, unilateral CMJs in different directions should be included in soccer-specific muscular power assessment and talent identification protocols, rather than the BV CMJ., (Copyright © 2017 Sports Medicine Australia. Published by Elsevier Ltd. All rights reserved.)

There have been considerable advances in monitoring training load in running-based team sports in recent years. Novel technologies nowadays offer ample opportunities to continuously monitor the activities of a player. These activities lead to internal biochemical stresses on the various physiological subsystems; however, they also cause internal mechanical stresses on the various musculoskeletal tissues. Based on the amount and periodization of these stresses, the subsystems and tissues adapt. Therefore, by monitoring external loads, one hopes to estimate internal loads to predict adaptation, through understanding the load-adaptation pathways. We propose a new theoretical framework in which physiological and biomechanical load-adaptation pathways are considered separately, shedding new light on some of the previously published evidence. We hope that it can help the various practitioners in this field (trainers, coaches, medical staff, sport scientists) to align their thoughts when considering the value of monitoring load, and that it can help researchers design experiments that can better rationalize training-load monitoring for improving performance while preventing injury.

During the competitive season, soccer players are likely exposed to numerous factors that may disrupt the process of sleep. The current investigation looked to evaluate a practical sleep hygiene strategy (10-min showering at ∼40°C before lights out), within a group of 11 youth soccer players in comparison to normal sleeping conditions (control). Each condition consisted of three days within a randomised crossover trial design. Sleep information was collected using a commercial wireless bedside sleep monitor. Measures of skin temperature were evaluated using iButton skin thermistors to establish both distal and proximal skin temperatures and distal to proximal gradient. The shower intervention elevated distal skin temperature by 1.1°C (95% CI: 0.1-2.1°C, p = .04) on average prior to lights out. The elevation in distal temperature was also present during the first 30-min following lights out (1.0°C, 95% CI: 0.4-1.6°C, p < .01). The distal to proximal gradient also showed a significant effect between the conditions within the first 30-min after lights out (0.7°C, 95% CI: 0.3-1.2°C, p < .01). On average the sleep latency of the youth soccer players was -7-min lower (95% CI: -13 to -2 min, p < .01) and sleep efficiency +2% higher (95% CI: 1-3%; p < .01) in the shower condition. These findings demonstrate that a warm shower performed before lights out may offer a practical strategy to promote thermoregulatory changes that may advance sleep onset latency and improve sleep efficiency in athletes.

The aim of the present study was to examine reliability and construct convergent validity of Player Load™ (PL) from trunk-mounted accelerometry, expressed as a cumulative measure and an intensity measure (PL · min - 1 ). Fifteen male participants twice performed an overground football match simulation that included four different multidirectional football actions (jog, side cut, stride and sprint) whilst wearing a trunk-mounted accelerometer inbuilt in a global positioning system unit. Results showed a moderate-to-high reliability as indicated by the intra-class correlation coefficient (0.806-0.949) and limits of agreement. Convergent validity analysis showed considerable between-participant variation (coefficient of variation range 14.5-24.5%), which was not explained from participant demographics despite a negative association with body height for the stride task. Between-task variations generally showed a moderate correlation between ranking of participants for PL (0.593-0.764) and PL · min - 1 (0.282-0.736). It was concluded that monitoring PL ® in football multidirectional actions presents moderate-to-high reliability, that between-participant variability most likely relies on the individual's locomotive skills and not their anthropometrics, and that the intensity of a task expressed by PL · min - 1 is largely related to the running velocity of the task.

Datson, N, Drust, B, Weston, M, Jarman, IH, Lisboa, P, and Gregson, W. Match physical performance of elite female soccer players during international competition. J Strength Cond Res 31(9): 2379-2387, 2017-The purpose of this study was to provide a detailed analysis of the physical demands of competitive international female soccer match play. A total of 148 individual match observations were undertaken on 107 outfield players competing in competitive international matches during the 2011-2012 and 2012-2013 seasons, using a computerized tracking system (Prozone Sports Ltd., Leeds, England). Total distance and total high-speed running distances were influenced by playing position, with central midfielders completing the highest (10,985 ± 706 m and 2,882 ± 500 m) and central defenders the lowest (9,489 ± 562 m and 1,901 ± 268 m) distances, respectively. Greater total very high-speed running distances were completed when a team was without (399 ± 143 m) compared to with (313 ± 210 m) possession of the ball. Most sprints were over short distances with 76% and 95% being less than 5 and 10 m, respectively. Between half reductions in physical performance were present for all variables, independent of playing position. This study provides novel findings regarding the physical demands of different playing positions in competitive international female match play and provides important insights for physical coaches preparing elite female players for competition.

Mechanisms mediating postexercise cold-induced increases in PGC-1α gene expression in human skeletal muscle are yet to be fully elucidated but may involve local cooling effects on AMPK and p38 MAPK-related signaling and/or increased systemic β-adrenergic stimulation. Therefore, we aimed to examine whether postexercise cold water immersion enhancement of PGC-1α mRNA is mediated through local or systemic mechanisms. Ten subjects completed acute cycling (8 × 5 min at ~80% peak power output) followed by seated-rest (CON) or single-leg cold water immersion (CWI; 10 min, 8°C). Muscle biopsies were obtained preexercise, postexercise, and 3 h postexercise from a single limb in the CON condition but from both limbs in CWI [thereby providing tissue from a CWI and nonimmersed limb (NOT)]. Muscle temperature decreased up to 2 h postexercise following CWI (-5°C) in the immersed limb, with lesser changes observed in CON and NOT (-3°C, P < 0.05). No differences between limbs were observed in p38 MAPK phosphorylation at any time point ( P < 0.05), whereas a significant interaction effect was present for AMPK phosphorylation ( P = 0.031). Exercise (CON) increased gene expression of PGC-1α 3 h postexercise (~5-fold, P < 0.001). CWI augmented PGC-1α expression above CON in both the immersed (CWI; ~9-fold, P = 0.003) and NOT limbs (~12-fold, P = 0.001). Plasma normetanephrine concentration was higher in CWI vs. CON immediately postimmersion (860 vs. 665 pmol/l, P = 0.034). We report for the first time that local cooling of the immersed limb evokes transcriptional control of PGC-1α in the nonimmersed limb, suggesting increased systemic β-adrenergic activation of AMPK may mediate, in part, postexercise cold induction of PGC-1α mRNA. NEW & NOTEWORTHY We report for the first time that postexercise cold water immersion of one limb also enhances PGC-1α expression in a contralateral, nonimmersed limb. We suggest that increased systemic β-adrenergic stimulation, and not localized cooling per se, exerts regulatory effects on local signaling cascades, thereby modulating PGC-1α expression. Therefore, these data have important implications for research designs that adopt contralateral, nonimmersed limbs as a control condition while also increasing our understanding of the potential mechanisms underpinning cold-mediated PGC-1α responses., (Copyright © 2017 the American Physiological Society.)

In an attempt to better identify and inform the energy requirements of elite soccer players, we quantified the energy expenditure (EE) of players from the English Premier League (n = 6) via the doubly labeled water method (DLW) over a 7-day in-season period. Energy intake (EI) was also assessed using food diaries, supported by the remote food photographic method and 24 hr recalls. The 7-day period consisted of 5 training days (TD) and 2 match days (MD). Although mean daily EI (3186 ± 367 kcals) was not different from (p > .05) daily EE (3566 ± 585 kcals), EI was greater (p < .05) on MD (3789 ± 532 kcal; 61.1 ± 11.4 kcal.kg -1 LBM) compared with TD (2956 ± 374 kcal; 45.2 ± 9.3 kcal.kg -1 LBM, respectively). Differences in EI were reflective of greater (p < .05) daily CHO intake on MD (6.4 ± 2.2 g.kg -1 ) compared with TD (4.2 ± 1.4 g.kg -1 ). Exogenous CHO intake was also different (p < .01) during training sessions (3.1 ± 4.4 g.h -1 ) versus matches (32.3 ± 21.9 g.h -1 ). In contrast, daily protein (205 ± 30 g.kg -1 , p = .29) and fat intake (101 ± 20 g, p = .16) did not display any evidence of daily periodization as opposed to g.kg -1 , Although players readily achieve current guidelines for daily protein and fat intake, data suggest that CHO intake on the day before and in recovery from match play was not in accordance with guidelines to promote muscle glycogen storage.

It is recommended that soccer players consume a high carbohydrate diet to augment performance. However, growing evidence suggests that there is a link between high free-sugar (FS) intake (>5% total energy intake; TEI) and metabolic diseases. Furthermore, foods that are often high in sugar, such as processed foods, are typically lacking in nutrient quality. We therefore analysed total-sugar, FS, dietary fibre, and micronutrient intake of players from an English Premier League academy under (U) 18 (n = 13), U15/16 (n = 25), and U13/14 (n = 21) using a 7-day food diary. Data were compared with current United Kingdom (UK) dietary reference value (DRV) for FS via a t test. The U13/14s (10% ± 18%) and U15/16s (11% ± 30%) both consumed higher amounts of FS in comparison with the UK DRV of 5% TEI (P < 0.01); conversely, the U18s did not exceed the DRV (5% ± 13%). Furthermore, FS intake of the U18s was significantly lower than the U13/14s and U15/16s (P < 0.01). Dietary fibre was below the DRV (25 g/day for U13/14 and U15/16s; 30 g/day for U18s) for all squads (19.0 ± 4.7, 19.6 ± 8.3, 17.1 ± 4.2 g/day, respectively), but not different between squads. Additionally, micronutrient reference intakes were generally met. In conclusion, we provide novel data on dietary sugar, fibre, and micronutrient intake within elite youth soccer players. We report an apparent "nutritional transition" from schoolboy to fulltime soccer player, with U18s showing a significantly lower intake of sugar in comparison with younger squads, and a similar intake of FS to the UK DRVs. Practitioners should target improving player education around sugar and fibre consumption.

Purpose: To determine the sensitivity of a range of potential fatigue measures to daily training load accumulated over the previous 2, 3, and 4 d during a short in-season competitive period in elite senior soccer players (N = 10)., Methods: Total highspeed-running distance, perceived ratings of wellness (fatigue, muscle soreness, sleep quality), countermovement-jump height (CMJ), submaximal heart rate (HRex), postexercise heart-rate recovery (HRR), and heart-rate variability (HRV: Ln rMSSD) were analyzed during an in-season competitive period (17 d). General linear models were used to evaluate the influence of 2-, 3-, and 4-d total high-speed-running-distance accumulation on fatigue measures., Results: Fluctuations in perceived ratings of fatigue were correlated with fluctuations in total high-speed-running-distance accumulation covered on the previous 2 d (r = -.31; small), 3 d (r = -.42; moderate), and 4 d (r = -.28; small) (P < .05). Changes in HRex (r = .28; small; P = .02) were correlated with changes in 4-d total high-speed-running-distance accumulation only. Correlations between variability in muscle soreness, sleep quality, CMJ, HRR%, and HRV and total high-speed-running distance were negligible and not statistically significant for all accumulation training loads., Conclusions: Perceived ratings of fatigue and HRex were sensitive to fluctuations in acute total high-speed-running-distance accumulation, although sensitivity was not systematically influenced by the number of previous days over which the training load was accumulated. The present findings indicate that the sensitivity of morning-measured fatigue variables to changes in training load is generally not improved when compared with training loads beyond the previous day's training.

The increase in competition demands in elite team sports over recent years has prompted much attention from researchers and practitioners to the monitoring of adaptation and fatigue in athletes. Monitoring fatigue and gaining an understanding of athlete status may also provide insights and beneficial information pertaining to player availability, injury, and illness risk. Traditional methods used to quantify recovery and fatigue in team sports, such as maximal physical-performance assessments, may not be feasible to detect variations in fatigue status throughout competitive periods. Faster, simpler, and nonexhaustive tests such as athlete self-report measures, autonomic nervous system response via heart-rate-derived indices, and to a lesser extent, jump protocols may serve as promising tools to quantify and establish fatigue status in elite team-sport athletes. The robust rationalization and precise detection of a meaningful fluctuation in these measures are of paramount importance for practitioners working alongside athletes and coaches on a daily basis. There are various methods for arriving at a minimal clinically important difference, but these have been rarely adopted by sport scientists and practitioners. The implementation of appropriate, reliable, and sensitive measures of fatigue can provide important information to key stakeholders in team-sport environments. Future research is required to investigate the sensitivity of these tools to fundamental indicators such as performance, injury, and illness.

Purpose: To investigate the relationship between whole-body accelerations and body-worn accelerometry during team-sport movements., Methods: Twenty male team-sport players performed forward running and anticipated 45° and 90° side-cuts at approach speeds of 2, 3, 4, and 5 m/s. Whole-body center-of-mass (CoM) accelerations were determined from ground-reaction forces collected from 1 foot-ground contact, and segmental accelerations were measured from a commercial GPS accelerometer unit on the upper trunk. Three higher-specification accelerometers were also positioned on the GPS unit, the dorsal aspect of the pelvis, and the shaft of the tibia. Associations between mechanical load variables (peak acceleration, loading rate, and impulse) calculated from both CoM accelerations and segmental accelerations were explored using regression analysis. In addition, 1-dimensional statistical parametric mapping (SPM) was used to explore the relationships between peak segmental accelerations and CoM-acceleration profiles during the whole foot-ground contact., Results: A weak relationship was observed for the investigated mechanical load variables regardless of accelerometer location and task (R 2 values across accelerometer locations and tasks: peak acceleration .08-.55, loading rate .27-.59, and impulse .02-.59). Segmental accelerations generally overestimated whole-body mechanical load. SPM analysis showed that peak segmental accelerations were mostly related to CoM accelerations during the first 40-50% of contact phase., Conclusions: While body-worn accelerometry correlates to whole-body loading in team-sport movements and can reveal useful estimates concerning loading, these correlations are not strong. Body-worn accelerometry should therefore be used with caution to monitor whole-body mechanical loading in the field.

Purpose: We tested the hypothesis that both post-exercise and passive cold water immersion (CWI) increases PGC-1α and VEGF mRNA expression in human skeletal muscle., Method: Study 1 Nine males completed an intermittent running protocol (8 × 3-min bouts at 90 % [Formula: see text], interspersed with 3-min active recovery (1.5-min at 25 % and 1.5-min at 50 % [Formula: see text]) before undergoing CWI (10 min at 8 °C) or seated rest (CONT) in a counterbalanced, randomised manner. Study 2 Ten males underwent an identical CWI protocol under passive conditions., Results: Study 1 PGC-1α mRNA increased in CONT (~3.4-fold; P < 0.001) and CWI (~5.9-fold; P < 0.001) at 3 h post-exercise with a greater increase observed in CWI (P < 0.001). VEGFtotal mRNA increased after CWI only (~2.4-fold) compared with CONT (~1.1-fold) at 3 h post-exercise (P < 0.01). Study 2 Following CWI, PGC-1α mRNA expression was significantly increased ~1.3-fold (P = 0.001) and 1.4-fold (P = 0.0004) at 3 and 6 h, respectively. Similarly, VEGF165 mRNA was significantly increased in CWI ~1.9-fold (P = 0.03) and 2.2-fold (P = 0.009) at 3 and 6 h post-immersion., Conclusions: Data confirm post-exercise CWI augments the acute exercise-induced expression of PGC-1α mRNA in human skeletal muscle compared to exercise per se. Additionally CWI per se mediates the activation of PGC-1α and VEGF mRNA expression in human skeletal muscle. Cold water may therefore enhance the adaptive response to acute exercise., Competing Interests: Conflicts of Interest: None declared. Funding No funding is declared.

Purpose: To quantify the accumulative training and match load during an annual season in English Premier League soccer players classified as starters (n = 8, started ≥60% of games), fringe players (n = 7, started 30-60% of games) and nonstarters (n = 4, started <30% of games). Methods Players were monitored during all training sessions and games completed in the 2013-14 season with load quantified using global positioning system and Prozone technology, respectively., Results: When including both training and matches, total duration of activity (10,678 ± 916, 9955 ± 947, 10,136 ± 847 min; P = .50) and distance covered (816.2 ± 92.5, 733.8 ± 99.4, 691.2 ± 71.5 km; P = .16) were not different between starters, fringe players, and nonstarters, respectively. However, starters completed more (all P < .01) distance running at 14.4-19.8 km/h (91.8 ± 16.3 vs 58.0 ± 3.9 km; effect size [ES] = 2.5), high-speed running at 19.9-25.1 km/h (35.0 ± 8.2 vs 18.6 ± 4.3 km; ES = 2.3), and sprinting at >25.2 km/h (11.2 ± 4.2 vs 2.9 ± 1.2 km; ES = 2.3) than nonstarters. In addition, starters also completed more sprinting (P < .01, ES = 2.0) than fringe players, who accumulated 4.5 ± 1.8 km. Such differences in total high-intensity physical work done were reflective of differences in actual game time between playing groups as opposed to differences in high-intensity loading patterns during training sessions. Conclusions Unlike total seasonal volume of training (ie, total distance and duration), seasonal high-intensity loading patterns are dependent on players' match starting status, thereby having potential implications for training program design.

Purpose: To quantify the mean daily changes in training and match load and any parallel changes in indicators of morningmeasured fatigue across in-season training weeks in elite soccer players., Methods: After each training session and match (TL), session ratings of perceived exertion (s-RPE) were recorded to calculate overall session load (RPE-TL) in 29 English Premier League players from the same team. Morning ratings of fatigue, sleep quality, and delayed-onset muscle soreness (DOMS), as well as submaximal exercise heart rate (HRex), postexercise heart-rate recovery (HRR%), and heart-rate variability (HRV) were recorded before match day and 1, 2, and 4 d postmatch. Data were collected for a median duration of 3 wk (range 1-13) and reduced to a typical weekly cycle including no midweek match and a weekend match day. Data were analyzed using withinsubject linear mixed models., Results: RPE-TL was approximately 600 arbitrary units (AU) (95% confidence interval 546-644) higher on match day than following day (P < .001). RPE-TL progressively decreased by »60 AU per day over the 3 days before a match (P < .05). Morning-measured fatigue, sleep quality, and DOMS tracked the changes in RPE-TL, being 35-40% worse on postmatch day vs prematch day (P < .001). Perceived fatigue, sleep quality, and DOMS improved by 17-26% from postmatch day to 3 d postmatch, with further smaller (7%-14%) improvements occurring between 4 d postmatch and prematch day (P < .01). There were no substantial or statistically significant changes in HRex, HRR%, or HRV over the weekly cycle (P > .05)., Conclusions: Morning-measured ratings of fatigue, sleep quality, and DOMS are clearly more sensitive than HR-derived indices to the daily fluctuations in session load experienced by elite soccer players in a standard in-season week.

While traditional approaches to dietary analysis in athletes have focused on total daily energy and macronutrient intake, it is now thought that daily distribution of these parameters can also influence training adaptations. Using 7-day food diaries, we quantified the total daily macronutrient intake and distribution in elite youth soccer players from the English Premier League in U18 (n = 13), U15/16 (n = 25) and U13/14 squads (n = 21). Total energy (43.1 ± 10.3, 32.6 ± 7.9, 28.1 ± 6.8 kcal·kg -1 ·day -1 ), CHO (6 ± 1.2, 4.7 ± 1.4, 3.2 ± 1.3 g·kg - 1 ·day -1 ) and fat (1.3 ± 0.5, 0.9 ± 0.3, 0.9 ± 0.3 g·kg- 1 ·day -1 ) intake exhibited hierarchical differences (p < .05) such that U13/14 > U15/16 > U18. In addition, CHO intake in U18s was lower (p < .05) at breakfast, dinner and snacks when compared with both squads but no differences were apparent at lunch. Furthermore, the U15/16s reported lower relative daily protein intake than the U13/14s and U18s (1.6 ± 0.3 vs. 2.2 ± 0.5, 2.0 ± 0.3 g·kg -1 ). A skewed distribution (p < .05) of daily protein intake was observed in all squads, with a hierarchical order of dinner (~0.6 g·kg -1 ) > lunch (~0.5 g·kg -1 ) > breakfast (~0.3 g·kg -1 ). We conclude elite youth soccer players do not meet current CHO guidelines. Although daily protein targets are achieved, we report a skewed daily distribution in all ages such that dinner > lunch > breakfast. Our data suggest that dietary advice for elite youth players should focus on both total daily macronutrient intake and optimal daily distribution patterns.

Prolonged unaccustomed exercise involving muscle lengthening (eccentric) actions can result in ultrastructural muscle disruption, impaired excitation-contraction coupling, inflammation and muscle protein degradation. This process is associated with delayed onset muscle soreness and is referred to as exercise-induced muscle damage. Although a certain amount of muscle damage may be necessary for adaptation to occur, excessive damage or inadequate recovery from exercise-induced muscle damage can increase injury risk, particularly in older individuals, who experience more damage and require longer to recover from muscle damaging exercise than younger adults. Furthermore, it is apparent that inter-individual variation exists in the response to exercise-induced muscle damage, and there is evidence that genetic variability may play a key role. Although this area of research is in its infancy, certain gene variations, or polymorphisms have been associated with exercise-induced muscle damage (i.e. individuals with certain genotypes experience greater muscle damage, and require longer recovery, following strenuous exercise). These polymorphisms include ACTN3 (R577X, rs1815739), TNF (-308 G>A, rs1800629), IL6 (-174 G>C, rs1800795), and IGF2 (ApaI, 17200 G>A, rs680). Knowing how someone is likely to respond to a particular type of exercise could help coaches/practitioners individualise the exercise training of their athletes/patients, thus maximising recovery and adaptation, while reducing overload-associated injury risk. The purpose of this review is to provide a critical analysis of the literature concerning gene polymorphisms associated with exercise-induced muscle damage, both in young and older individuals, and to highlight the potential mechanisms underpinning these associations, thus providing a better understanding of exercise-induced muscle damage.