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Pinholins are a family of lytic membrane proteins responsible for the lysis of the cytosolic membrane in host cells of double stranded DNA bacteriophages. Protein-lipid interactions have been shown to influence membrane protein topology as well as its function. This work investigated the interactions of pinholin with the phospholipid bilayer while in active and inactive confirmations to elucidate the different interactions the two forms have with the bilayer. Pinholin incorporated into deuterated DMPC-d 54 lipid bilayers, along with 31P and 2H solid state NMR (SS-NMR) spectroscopy were used to probe the protein-lipid interactions with the phosphorus head group at the surface of the bilayer while interactions with the 2H nuclei were used to study the hydrophobic core. A comparison of the 31P chemical shift anisotropy (CSA) values of the active S2168 pinholin and inactive S21IRS pinholin indicated stronger head group interactions for the pinholin in its active form when compared to that of the inactive form supporting the model of a partially externalized peripheral transmembrane domain (TMD) of the active S2168 instead of complete externalized TMD1 as suggested by Ahammad et al. JPC B 2019. The 2H quadrupolar splitting analysis showed a decrease in spectral width for both forms of the pinholin when compared to the empty bilayers at all temperatures. In this case the decrease in the spectral width of the inactive S21IRS form of the pinholin showed stronger interactions with the acyl chains of the bilayer. The presence of the inactive form's additional TMD within the membrane was supported by the loss of peak resolution observed in the 2H NMR spectra. Unlabelled Image • Active S2168 and inactive S21IRS pinholin interact differently with the membrane. • Active S2168 pinholin shows a higher degree of interactions with lipid headgroups • Inactive S21IRS perturbs the hydrophobic core of the membrane more than active S2168 • Solid-state NMR probed the difference between an active and inactive protein [ABSTRACT FROM AUTHOR]

Global growth in variable renewable generation has brought significant attention to the challenge of balancing electricity supply and demand. However, inter-annual variability of energy resources has only recently begun to feature in energy system assessments and receives limited recognition in policy discussion, let alone policy design. Meteorological reanalysis datasets that blend modern modelling techniques with historic weather records are seeing increased application in energy system studies. This practice offers insights for market and policy design implications as governments seek to manage the changing energy landscape, as seen with the UK's introduction of the Electricity Market Reform policy package. Here we apply a concise, Load Duration Curve based approach to consider the market and policy implications of increasing variability in the Great Britain (GB) energy system. Our findings emphasise the growing inter-annual variability in operating opportunity for residual mid-merit and even baseload generation, alongside implications for capacity assurance approaches. The growth in wind generation is seen to bring an accompanying opportunity for increased solar generation, with its lower inter-annual variability and largely uncorrelated annual characteristic. The results underscore the need for an increased recognition of inter-annual variability when addressing market design and incentive mechanisms. • Meteorological reanalysis datasets benefit energy system studies. • Load duration curve approach complements sophisticated system models. • Inter-annual variability has implications for energy and capacity (power) based markets. • Blended renewables solutions help mitigate inter-annual variability. [ABSTRACT FROM AUTHOR]

When rewards are available, people expend more energy, increasing their motivational vigor. In theory, incentives might drive behavior for two distinct reasons: First, they increase expected reward; second, they increase the difference in subjective value between successful and unsuccessful performance, which increases contingency--the degree to which action determines outcome. Previous studies of motivational vigor have never compared these directly. Here, we indexed motivational vigor by measuring the speed of eye movements toward a target after participants heard a cue indicating how outcomes would be determined. Eye movements were faster when the cue indicated that monetary rewards would be contingent on performance than when the cue indicated that rewards would be random. But even when the cue indicated that a reward was guaranteed regardless of speed, movement was still faster than when no reward was available. Motivation by contingent and certain rewards was uncorrelated across individuals, which suggests that there are two separable, independent components of motivation. Contingent motivation generated autonomic arousal, and unlike noncontingent motivation, was effective with penalties as well as rewards. [ABSTRACT FROM AUTHOR]

Atmospheric reanalyses are widely used for understanding the past and present climate. They have become increasingly used within the renewable energy sector for assessing wind and solar resources for different regions of the globe in conjunction with observations. Mexico is a country with considerable potential for wind energy production, especially around coastal sites and therefore the characterization of wind resource in these areas of the country is imperative for the most beneficial use of these resources. In this study, we assess how well three global reanalyses, namely ERA-Interim, ERA5 and MERRA-2, can reproduce wind observations at a number of key sites across the country. We find that the reanalyses' ability to reproduce these observations is highly variable between different regions in Mexico. Correlation coefficients are around 0.9 in the south of the country where the winds are strongest, but much lower (around 0.5) in Baja California Sur due to the complex coastal topography of the region. ERA5 outperforms ERA-Interim and MERRA-2 consistently across the vast majority of sites and so this reanalysis is recommended for local wind power studies. The consistently improved performance compared with ERA-Interim shows the value of the increased spatial resolution of ERA5. However, in the south and east of Mexico, despite having the highest correlations, ERA5 also has the largest bias, meaning that it underestimates winds consistently across most of the country. Poor correlations between ERA5 and the observations in Veracruz are considered as a case study to understand potential drivers of low wind biases. [ABSTRACT FROM AUTHOR]

Social cohesion relies on prosociality in increasingly aging populations. Helping other people requires effort, yet how willing people are to exert effort to benefit themselves and others, and whether such behaviors shift across the life span, is poorly understood. Using computational modeling, we tested the willingness of 95 younger adults (18–36 years old) and 92 older adults (55–84 years old) to put physical effort into self- and other-benefiting acts. Participants chose whether to work and exert force (30%–70% of maximum grip strength) for rewards (2–10 credits) accrued for themselves or, prosocially, for another. Younger adults were somewhat selfish, choosing to work more at higher effort levels for themselves, and exerted less force in prosocial work. Strikingly, compared with younger adults, older people were more willing to put in effort for others and exerted equal force for themselves and others. Increased prosociality in older people has important implications for human behavior and societal structure. [ABSTRACT FROM AUTHOR]

Patients with small vessel cerebrovascular disease frequently suffer from apathy, a debilitating neuropsychiatric syndrome, the underlying mechanisms of which remain to be established. Here we investigated the hypothesis that apathy is associated with disrupted decision making in effort-based decision making, and that these alterations are associated with abnormalities in the white matter network connecting brain regions that underpin such decisions. Eighty-two patients with MRI evidence of small vessel disease were assessed using a behavioural paradigm as well as diffusion weighted MRI. The decision-making task involved accepting or rejecting monetary rewards in return for performing different levels of physical effort (hand grip force). Choice data and reaction times were integrated into a drift diffusion model that framed decisions to accept or reject offers as stochastic processes approaching a decision boundary with a particular drift rate. Tract-based spatial statistics were used to assess the relationship between white matter tract integrity and apathy, while accounting for depression. Overall, patients with apathy accepted significantly fewer offers on this decision-making task. Notably, while apathetic patients were less responsive to low rewards, they were also significantly averse to investing in high effort. Significant reductions in white matter integrity were observed to be specifically related to apathy, but not to depression. These included pathways connecting brain regions previously implicated in effort-based decision making in healthy people. The drift rate to decision parameter was significantly associated with both apathy and altered white matter tracts, suggesting that both brain and behavioural changes in apathy are associated with this single parameter. On the other hand, depression was associated with an increase in the decision boundary, consistent with an increase in the amount of evidence required prior to making a decision. These findings demonstrate altered effort-based decision making for reward in apathy, and also highlight dissociable mechanisms underlying apathy and depression in small vessel disease. They provide clear potential brain and behavioural targets for future therapeutic interventions, as well as modelling parameters that can be used to measure the effects of treatment at the behavioural level. [ABSTRACT FROM AUTHOR]