394 results on '"Dimkić Ivica"'
Search Results
152. Karakterizacija antimikrobnih supstanci prirodnih izolata Bacillus sp. za primenu u biološkoj kontroli fitopatogenih bakterija i gljiva
- Author
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Dimkić, Ivica Z., Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, Lozo, Jelena, and Stević, Tatjana
- Subjects
lipopeptidna jedinjenja ,antimicrobial activity ,synergism ,biological control ,Bacillus ,antimikrobna aktivnost ,biološka kontrola ,lipopeptides ,sinergizam - Abstract
Bolesti biljaka izazvane infekcijama patogenim bakterijama i gljivama mogu dovesti do smanjenja kapaciteta biljnog rasta ili naneti mnogo ozbiljniju štetu dovodeći do smrti biljaka i značajnih gubitaka u proizvodnji hrane. Veliki broj istraživanja je u poslednje vreme posvećen proučavanju roda Bacillus kao producenta sekundarnih metabolita u kontroli različitih biljnih patogena. Analizom diverziteta gena odgovornih za produkciju neribozomalno sintetisanih lipopeptida kolekcije od 205 Bacillus sp. izolata utvrđeno je da je za najveći broj izolata (171) ustanovljen DNK fragment očekivane dužine, koji odgovara bacilomicin D sintetazi, dok su u sličnom broju izolata dobijeni fragmenti očekivanih dužina za kurstakinske (154) i surfaktinske operone (142), a prisustvo iturinskog operona ustanovljeno je kod 84, odnosno 59 izolata u slučaju gena za fengicin sintetazu. Prisustvo svih pet biosintetičkih operona je potvrđeno kod 11 izolata. Najveća produkcija antimikrobnih jedinjenja za pet izabranih izolata je pokazana u eksponencijalnoj ili ranoj stacionarnoj fazi rasta. Prikazan je jak antifungalni efekat lipopeptidnih ekstrakata prema više od 30 testiranih gljiva, sa najnižim zabeleženim minimalnim inhibitornim koncentracijama od 0,008 mg/ml protiv Fusarium semitectum, kao i izražen antibakterijski potencijal, naročito protiv fitopatogenih vrsta rodova Xanthomonas i Pseudomonas, sa najnižom minimalnom inhibitornom koncentracijom od 0,06 mg/ml. Proučavanje interakcija pojedinačnih lipopeptidnih ekstrakata u međusobnim, kao i u kombinacijama sa etarskim uljima, ukazalo je na postojanje sinergističkog efekta za neke kombinacije u in vitro, odnosno aditivnog efekta u in situ uslovima, na bakterijskim i fungalnim patogenima. Utvrđivanjem direktnog antagonističkog efekta lipopeptidnih jedinjenja in planta, ustanovljena je značajna redukcija nekroze tkiva listova Arabidopsis thaliana L. i Beta vulgaris L., tretiranih bakterijskim patogenima. Takođe, u in situ uslovima postignuta je značajna redukcija nekroze tkiva ploda jabuke izazvana fungalnom infekcijom... Plant diseases caused by infection with pathogenic bacteria and fungi can lead to the reduction in the capacity of plant growth or can cause far more serious damage, leading to the death of plants and significant losses in food production. Numerous recent studies are devoted to the genus Bacillus as a producer of secondary metabolites in the control of different plant pathogens. In the analysis of diversity of genes responsible for the nonribosomal synthesized lipopeptides in collections of 205 Bacillus sp. isolates, for the largest number of isolates (171) the DNA PCR fragment of the expected length, corresponding to bacillomycin D synthases was obtained, while in a similar number of isolates fragments of the expected length for kurstakin (154) and surfactin operons (142) were obtained. The presence of iturin operon in the 84 or 59 isolates in the case of the gene for fengycin synthetase, was also found. The presence of all five biosynthetic operons in the 11 isolates was confirmed as well. The maximum production of antimicrobial compounds for five selected isolates in exponential or early stationary phase of growth was determined. Strong antifungal effect of lipopeptide extracts towards more than 30 fungi tested, with lowest recorded minimal inhibitory concentrations of 0,008 mg/ml against Fusarium semitectum, as well as expressed antibacterial potential, in particular against phytopathogenic species of the genera Pseudomonas and Xanthomonas, with the lowest minimum inhibitory concentration of 0,06 mg/ml was determined. In the analysis of the interaction of individual lipopeptide extracts mutually, as well as in combination with essential oils, the existence of a synergistic effect in vitro respectively additive effect in situ, on bacterial and fungal pathogens, for some combinations were shown. Establishing a direct antagonistic effect of lipopeptide compounds in planta, significant reduction in necrotic lesions of leaves of Arabidopsis thaliana L. and Beta vulgaris L. treated with bacterial pathogens was obtained. Also, a significant reduction in necrotic lesions of apple fruit caused by fungal infection in situ was determined...
- Published
- 2015
153. Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosaisolates in Southern Serbia
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Milojković, Marko, Nenadović, Željka, Stanković, Slaviša, Božić, Dragana D., Nedeljković, Nataša Stanković, Ćirković, Ivana, Petrović, Marija, and Dimkić, Ivica
- Abstract
Drug resistance of Pseudomonas aeruginosais a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonasspp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of isolates. A total of 77.66 % isolates were mostly weak and moderate biofilm producers. Isolates were susceptible to colistin (100 %), aztreonam (97.87 %), imipenem (91.49 %), doripenem (90.43 %), and meropenem (84.04 %). MICs values confirmed susceptibility to ceftazidime and cefepime and singled out meripenem as the most effective inhibitor. Most isolates were resistant to aminoglycosides and fluoroquinolones. Only two isolates produced ESBL, eight were carbapenemase producers, and five isolates produced MBLs. Twenty-nine isolates were multidrug-resistant; 82.8 % of which produced both pigments, 58.3 % were non-typeable, while the P6 and P11 serotypes were equally distributed (16.7 %). Thirteen MDR isolates were strong enzyme producers. RAPD PCR analysis using primer 272 proved the best at discriminatory fingerprinting for Pseudomonasisolates, as it allocated 12 clusters. A correlation between DNA patterns and antibiotic resistance, production of pigments, serotypes distribution, and biofilm formation was not observed, and only confirmed higher genetic heterogeneity among P. aeruginosaisolates, which suggests that other molecular methods are needed to reveal potential relations between genotypic patterns and phenotypic characteristics.
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- 2020
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154. Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097
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Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, and Stanković, Slaviša
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- 2016
155. Phenolic profiles and antimicrobial activity of various plant resins as potential botanical sources of Serbian propolis
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Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, and Stanković, Slaviša
- Abstract
Extensive employment of plant resins and propolis, as an antiseptic agents dating from ancient times in numerous cultures indicating that it may have antimicrobial and other biological properties. Resins from deciduous trees from the Populus and Salix genera, several fruit trees from the genus Prunus and a few other species were analyzed for their phenolic composition and antibacterial activity. Phenolic profiling of the plant resins was performed by high-performance thin-layer chromatography (HPTLC) and ultra-high-performance liquid chromatography (UHPLC) coupled with hybrid mass spectrometry. Antimicrobial activity against seven bacterial species was determined by minimum inhibitory concentration (MIC) assays and bioautography. The synergism, additivism, and antagonism of phenolic compounds were used to define the nature and type of interactions. Populus spp. showed higher amounts of p-hydroxybenzoic acid, p-coumaric acid, caffeic acid, chrysin, apigenin, quercetin, pinocembrin, pinobanksin and galangin, which confirmed the botanical origin of the orange and blue types of propolis. In addition, Gram-positive bacteria exhibited high susceptibility to poplar samples while being resistant to samples from other origins. Cherry bud samples had high amounts of naringenin and showed strong activity against Bacillus subtilis and Listeria monocytogenes. The combinations of tested phenolics showed mainly additive or indifferent effects. (C) 2016 Elsevier B.V. All rights reserved.
- Published
- 2016
156. Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)
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Stanković, Slaviša, Dimkić, Ivica, Vujisić, Ljubodrag V., Pavković-Lučić, Sofija, Jovanović, Zvezdana, Stević, Tatjana, Sofrenić, Ivana V., Mitić, Bojan M., Tomić, Vladimir T., Stanković, Slaviša, Dimkić, Ivica, Vujisić, Ljubodrag V., Pavković-Lučić, Sofija, Jovanović, Zvezdana, Stević, Tatjana, Sofrenić, Ivana V., Mitić, Bojan M., and Tomić, Vladimir T.
- Abstract
The chemical defence of the millipede Pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. In total, independently of sex, 44 compounds were identified. All compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. The relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. The two dominant quinones in both sexes were 2-methyl-1,4,-benzoquinone and 2-methoxy-3-methyl-1,4-benzoquinone. Antibacterial and antifungal activity of the defensive secretion was evaluated in vitro against seven bacterial strains and eight fungal species. With the aid of a dilution technique, the antimicrobial potential of the secretion and high sensitivity of all tested strains were confirmed. The lowest minimum concentrations of these compounds (0.20-0.25 mg/mL) were sufficient for inhibition of Aeromonas hydrophila, Listeria monocytogenes and Methicillin resistant Staphylococcus aureus (MRSA). The growth of eight tested fungal species was inhibited by slightly lower concentrations of the secretion, with Fusarium equisetias the most sensitive fungus and Aspergillus flavus as the most resistant. Values of MIC and MFC in the employed microdilution assay ranged from 0.10 to above 0.35 mg/m L. The given extract contains antimicrobial components potentially useful as therapeutic agents in the pharmaceutical and agricultural industries.
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- 2016
157. Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065
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Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, Dimkić, Ivica, Ristivojević, Petar, Janakiev, Tamara, Berić, Tanja, Trifković, Jelena, Milojković-Opsenica, Dušanka, and Stanković, Slaviša
- Published
- 2016
158. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics
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Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, Stanković, Slaviša, Ristivojević, Petar, Dimkić, Ivica, Trifković, Jelena, Berić, Tanja, Vovk, Irena, Milojković-Opsenica, Dušanka, and Stanković, Slaviša
- Abstract
New information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as High Performance Thin-Layer Chromatography (HPTLC) fingerprinting. This study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of Serbian origin against various bacterial species. A linear multivariate calibration technique, using Partial Least Squares, was used to extract the relevant information from the chromatographic fingerprints, i.e. to indicate peaks which represent phenolic compounds that are potentially responsible for the antimicrobial capacity of the samples. In addition, direct bioautography was performed to localize the antibacterial activity on chromatograms. The biological activity of the propolis samples against various bacterial species was determined by a minimum inhibitory concentration assay, confirming their affiliation with the European poplar type of propolis and revealing the existence of two types (blue and orange) according to botanical origin. The strongest antibacterial activity was exhibited by sample 26 against Staphylococcus aureus, with a MIC value of 0.5 mg/mL, and Listeria monocytogenes, with a MIC as low as 0.1 mg/mL, which was also the lowest effective concentration observed in our study. Generally, the orange type of propolis shows higher antimicrobial activity compared to the blue type. PLS modelling was performed on the HPTLC data set and the resulting models might qualitatively indicate compounds that play an important role in the activity exhibited by the propolis samples. The most relevant peaks influencing the antimicrobial activity of propolis against all bacterial strains were phenolic compounds at RF values of 0.37, 0.40, 0.45, 0.51, 0.60 and 0.70. The knowledge gained through this study could be important for attributing the antimicrobial activity of propolis to specific chemical compoun
- Published
- 2016
159. Hemijski sastav i inhibitorna aktivnost odabranih etarskih ulja na gljive izolovane sa lekovitog bilja
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Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, and Berić, Tanja
- Subjects
microdilution method ,lekovito bilje ,antifungal activity ,etarska ulja ,phytopathogenic andsaprophytic fungi ,antifungalna aktivnost ,mikrodilucioni metod ,essential oils ,fitopatogene isaprofitne gljive - Abstract
In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses. Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima. Projekat ministarstva br. III 46013 i br. OI 17302
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- 2014
160. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics
- Author
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Ristivojević, Petar, primary, Dimkić, Ivica, additional, Trifković, Jelena, additional, Berić, Tanja, additional, Vovk, Irena, additional, Milojković-Opsenica, Dušanka, additional, and Stanković, Slaviša, additional
- Published
- 2016
- Full Text
- View/download PDF
161. Karakterizacija antimikrobnih supstanci prirodnih izolata Bacillus sp. za primenu u biološkoj kontroli fitopatogenih bakterija i gljiva
- Author
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Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, Lozo, Jelena, Stević, Tatjana, Dimkić, Ivica Z., Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, Lozo, Jelena, Stević, Tatjana, and Dimkić, Ivica Z.
- Abstract
Bolesti biljaka izazvane infekcijama patogenim bakterijama i gljivama mogu dovesti do smanjenja kapaciteta biljnog rasta ili naneti mnogo ozbiljniju štetu dovodeći do smrti biljaka i značajnih gubitaka u proizvodnji hrane. Veliki broj istraživanja je u poslednje vreme posvećen proučavanju roda Bacillus kao producenta sekundarnih metabolita u kontroli različitih biljnih patogena. Analizom diverziteta gena odgovornih za produkciju neribozomalno sintetisanih lipopeptida kolekcije od 205 Bacillus sp. izolata utvrđeno je da je za najveći broj izolata (171) ustanovljen DNK fragment očekivane dužine, koji odgovara bacilomicin D sintetazi, dok su u sličnom broju izolata dobijeni fragmenti očekivanih dužina za kurstakinske (154) i surfaktinske operone (142), a prisustvo iturinskog operona ustanovljeno je kod 84, odnosno 59 izolata u slučaju gena za fengicin sintetazu. Prisustvo svih pet biosintetičkih operona je potvrđeno kod 11 izolata. Najveća produkcija antimikrobnih jedinjenja za pet izabranih izolata je pokazana u eksponencijalnoj ili ranoj stacionarnoj fazi rasta. Prikazan je jak antifungalni efekat lipopeptidnih ekstrakata prema više od 30 testiranih gljiva, sa najnižim zabeleženim minimalnim inhibitornim koncentracijama od 0,008 mg/ml protiv Fusarium semitectum, kao i izražen antibakterijski potencijal, naročito protiv fitopatogenih vrsta rodova Xanthomonas i Pseudomonas, sa najnižom minimalnom inhibitornom koncentracijom od 0,06 mg/ml. Proučavanje interakcija pojedinačnih lipopeptidnih ekstrakata u međusobnim, kao i u kombinacijama sa etarskim uljima, ukazalo je na postojanje sinergističkog efekta za neke kombinacije u in vitro, odnosno aditivnog efekta u in situ uslovima, na bakterijskim i fungalnim patogenima. Utvrđivanjem direktnog antagonističkog efekta lipopeptidnih jedinjenja in planta, ustanovljena je značajna redukcija nekroze tkiva listova Arabidopsis thaliana L. i Beta vulgaris L., tretiranih bakterijskim patogenima. Takođe, u in situ uslovima postignuta j, Plant diseases caused by infection with pathogenic bacteria and fungi can lead to the reduction in the capacity of plant growth or can cause far more serious damage, leading to the death of plants and significant losses in food production. Numerous recent studies are devoted to the genus Bacillus as a producer of secondary metabolites in the control of different plant pathogens. In the analysis of diversity of genes responsible for the nonribosomal synthesized lipopeptides in collections of 205 Bacillus sp. isolates, for the largest number of isolates (171) the DNA PCR fragment of the expected length, corresponding to bacillomycin D synthases was obtained, while in a similar number of isolates fragments of the expected length for kurstakin (154) and surfactin operons (142) were obtained. The presence of iturin operon in the 84 or 59 isolates in the case of the gene for fengycin synthetase, was also found. The presence of all five biosynthetic operons in the 11 isolates was confirmed as well. The maximum production of antimicrobial compounds for five selected isolates in exponential or early stationary phase of growth was determined. Strong antifungal effect of lipopeptide extracts towards more than 30 fungi tested, with lowest recorded minimal inhibitory concentrations of 0,008 mg/ml against Fusarium semitectum, as well as expressed antibacterial potential, in particular against phytopathogenic species of the genera Pseudomonas and Xanthomonas, with the lowest minimum inhibitory concentration of 0,06 mg/ml was determined. In the analysis of the interaction of individual lipopeptide extracts mutually, as well as in combination with essential oils, the existence of a synergistic effect in vitro respectively additive effect in situ, on bacterial and fungal pathogens, for some combinations were shown. Establishing a direct antagonistic effect of lipopeptide compounds in planta, significant reduction in necrotic lesions of leaves of Arabidopsis thaliana L. and Beta vulga
- Published
- 2015
162. Identification and antibiotic resistance of Bacillus spp. isolates from natural samples.
- Author
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Berić, Tanja, Biočanin, Marjan, Stanković, Slaviša, Dimkić, Ivica, Janakiev, Tamara, Fira, Đorđe, and Lozo, Jelena
- Subjects
BACILLUS (Bacteria) ,INHIBITORY Concentration 50 ,RIBOSOMAL RNA genetics ,ERYTHROMYCIN ,RNA sequencing ,CLINDAMYCIN - Abstract
Identification of 33 Bacillus spp. isolates from different environmental samples collected from the territory of Serbia was performed by sequencing of the 5'-hypervariable section of 16S rRNA gene. Eight species were identified within four phylogenetic groups: B. pumilus, B. megaterium, B. subtilis and B. cereus. Determination of their antibiotic resistance was performed using the minimum inhibitory concentration (MIC) assay. We found that just one isolate was resistant to gentamicin, 9 were resistant to clindamycin and all were resistant to vancomycin. Based on the profile of resistance, the isolates were categorized into 4 categories. In silico analysis of the erythromycin-resistance (erm) gene for clindamycin resistance showed their distribution between related and nonrelated soil and human isolates including different species of Bacillus genera. This finding indicates that Bacillus spp. from the environment could be a source of resistance to clindamycin. The potential for the presence and spread of resistance determinants in the soil and similar ecosystems exists so that monitoring of antibiotic resistance genes in nonpathogenic Bacillus strains from the environment is advised. [ABSTRACT FROM AUTHOR]
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- 2018
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163. Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds
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Dimkić, Ivica, primary, Berić, Tanja, additional, Stević, Tatjana, additional, Pavlović, Snežana, additional, Šavikin, Katarina, additional, Fira, Djordje, additional, and Stanković, Slaviša, additional
- Published
- 2015
- Full Text
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164. Chemical composition and inhibitory activity of selected essential oils against fungi isolated from medicinal plants
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Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, Berić, Tanja, Stević, Tatjana, Stanković, Slaviša, Šavikin, Katarina, Gođevac, Dejan, Dimkić, Ivica, Soković, Marina, and Berić, Tanja
- Abstract
In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses., Poslednjih godina velika pažnja poklanja se biološkoj kontroli to jest primeni prirodnih produkata u zaštiti, kako poljoprivrednih kultura, tako i lekovitog bilja od kontaminacije fitopatogenim i saprofitnim gljivama. U tom smislu, ispitivan je antifungalni potencijal šest etarskih ulja uz određivanje kompletnog kvalitativnog i kvantitativnog hemijskog sastava primenom GC-FID i GC-MS analiza. Dok se korišćeni uzorci etarskih ulja ruzmarina, žalfije, španske žalfije i crnog bibera isključivo sastoje od monoterpena, u ulju vetivera potpuno dominiraju seskviterpeni. U etarskom ulju cimeta najzastupljenija komponneta je bila (E)- cinemaldehid. Primenom in vitro mikrodilucione metode, utvrđeno je da su sva ulja bila aktivna u inhibiciji rasta 21 testirane, pre- i post-žetvene, fitopatogene i saprofitne gljive, sa razlikama u efikasnosti. MIC i MFC vrednosti su varirale u opsegu od 1,2 mg ml-1 do 22,6 mg ml-1, u zavisnosti od ispitivanog ulja. Najbolja antifungalna aktivnost utvrđena je za ulje ruzmarina, nešto slabija za ulja crnog bibera i cimeta, dok je ulje vetivera ispoljilo najslabiju aktivnost. Etarska ulja obe vrste žalfija ispoljila su značajan antifungalni potencijal. Etarska ulja koja poseduju značajan antifungalni potencijal su dobri kandidati za nastavak istraživanja u pravcu njihove primene u prevenciji i zaštiti useva lekovitog bilja, kao i njihovih semena i osušenih biljnih droga od gljivičnih infekcija, i u polju i u skladištima.
- Published
- 2014
165. GENOTYPING OF BACILLUS SPP. ISOLATE COLLECTION FROM NATURAL SAMPLES.
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DRAGANIĆ, Veselin, LOZO, Jelena, BIOČANIN, Marjan, DIMKIĆ, Ivica, GARALEJIĆ, Eliana, FIRA, Djordje, STANKOVIĆ, Slaviša, and BERIĆ, Tanja
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GEL electrophoresis ,BACILLUS (Bacteria) ,BACTERIA phylogeny ,NUCLEOTIDE sequencing ,RAPD technique - Abstract
Copyright of Genetika (0534-0012) is the property of Serbian Genetics Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
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166. Antifungal activity of selected essential oils against fungi isolated from medicinal plant
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Stević, Tatjana, primary, Berić, Tanja, additional, Šavikin, Katarina, additional, Soković, Marina, additional, Gođevac, Dejan, additional, Dimkić, Ivica, additional, and Stanković, Slaviša, additional
- Published
- 2014
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167. Stability andin vitroantimicrobial efficacy of a nanopropolis formulation intended for intramammary treatment of bovine mastitis
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Troncarelli, Marcella Zampoli, primary, Langoni, Helio, additional, Brandão, Humberto de Mello, additional, Dimkić, Ivica, additional, Stanković, Slaviša, additional, and Ribeiro, Andréa Rentz, additional
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- 2014
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168. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi
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Dimkić, Ivica, primary, Živković, Svetlana, additional, Berić, Tanja, additional, Ivanović, Žarko, additional, Gavrilović, Veljko, additional, Stanković, Slaviša, additional, and Fira, Djordje, additional
- Published
- 2013
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169. ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP.
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Stanojević, Olja, Milijašević-Marčić, Svetlana, Potočnik, Ivana, Stepanović, Miloš, Dimkić, Ivica, Stanković, Slaviša, and Berić, Tanja
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BACILLUS subtilis ,BACILLUS amyloliquefaciens ,BACILLUS licheniformis ,BACILLUS pumilus ,TRICHODERMA ,PATHOGENIC fungi ,FUNGAL growth - Abstract
The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of T. aggressivum f. europaeum, T. harzianum and T. koningii, while only 13 isolates inhibited the growth of T. atroviride. T. aggressivum f. europaeum proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to Bacillus subtilis, B. amyloliquefaciens, B. licheniformis and B. pumilus species. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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170. Phytochemical screening of the olive variety 'Istrska Belica' infected by fungus Venturia oleaginea.
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Janaćković, Pedja, Gašić, Uroš, Gavrilović, Milan, Dimkić, Ivica, Hladnik, Matjaž, Arbeiter, Alenka Baruca, and Bandelj, Dunja
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VERTICILLIUM dahliae ,OLIVE ,QUINIC acid ,MORINGA oleifera ,ORGANIC acids ,PLANT defenses ,RICE diseases & pests - Published
- 2022
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171. Stability and in vitro antimicrobial efficacy of a nanopropolis formulation intended for intramammary treatment of bovine mastitis.
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Troncarelli, Marcella Z., Langoni, Helio, de Mello Brandão, Humberto, Dimkić, Ivica, Stanković, Slaviša, and Ribeiro, Andréa Rentz
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BOVINE mastitis ,TREATMENT of cattle diseases ,ANTI-infective agents ,DRUG efficacy ,PROPOLIS ,NANOSTRUCTURED materials ,BACTERICIDES ,ORGANIC dairy farming - Abstract
Copyright of Revista Brasileira de Higiene e Sanidade Animal is the property of Associacao Cientifica dos Medicos Veterinarios do Ceara and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2014
- Full Text
- View/download PDF
172. Antibacterial activity of herbal extracts towards uropathogenic Enterococcus isolates as a natural approach in control of urinary tract infections.
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Dimkić, Ivica, Gobin, Ivana, Begić, Gabrijela, Antić, Davorka Repac, Ristivojević, Petar, Jurica, Karlo, Berić, Tanja, Lozo, Jelena, Abram, Maja, and Stanković, Slaviša
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ENTEROCOCCAL infections ,URINARY tract infections ,GALLIC acid ,LINEZOLID ,ENTEROCOCCUS ,ENTEROCOCCUS faecium ,ENTEROCOCCUS faecalis ,STRAWBERRIES - Abstract
[Display omitted] • A total of 4 different herbal extracts were analyzed against 96 Enterococcus uroisolates. • Phenolic composition, antimicrobial and anti-biofilm activity were determined. • The most of Enterococcus faecium isolates were recognized as multiple resistant. • More than 30 % of Enterococcus faecalis isolates have shown moderately biofilm production. • Synergism in antibiosis was detected among two herbal extract combinations. Antibacterial and antibiofilm efficacy through a synergistic effect of herbal leaf extracts of bearberry (BE), bearberry tea (BTE), parsley (PE), and the strawberry tree (STE) was evaluated to discover an alternative approach to control urinary tract infections caused by Enterococci. UHPLC–DAD MS/MS analysis indicated that herbal extracts were rich in ellagic and gallic acids, catechin, quercetin, and arbutin, which are strong antioxidants. Mostly, Enterococcus faecalis isolates showed resistance only to gentamicin and/or norfloxacin (26.3 %) and moderate biofilm production (31.5 %), while 12 isolates of Enterococcus faecium showed multiple antibiotic resistance. The minimum inhibitory concentration for the majority of isolates was 1.25 mg ml
−1 for STE, but 2.5 mg ml−1 for BE and BTE. Although individual extracts gave higher MIC values, for most isolates, a synergistic effect at lower concentrations was achieved, when BE or BTE (0.625 mg ml−1 ) was combined with STE (0.31 mg ml−1 ). The BTE extract was shown to have the strongest individual effect on initial adhesiveness and on biofilm formation to all selected isolates, while the synergism of BTE and STE caused significant biofilm inhibition at all concentrations tested. The synergistic effect achieved of the tested extract combinations might be a starting point in the development of alternative products, effective against antibiotic resistant Enterococcus isolates. [ABSTRACT FROM AUTHOR]- Published
- 2021
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173. Insights into Endophytic and Rhizospheric Bacteria of Five Sugar Beet Hybrids in Terms of Their Diversity, Plant-Growth Promoting, and Biocontrol Properties.
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Petrović, Marija, Janakiev, Tamara, Grbić, Milica Ljaljević, Unković, Nikola, Stević, Tatjana, Vukićević, Slavoljub, and Dimkić, Ivica
- Abstract
Sugar beet is the most important crop for sugar production in temperate zones. The plant microbiome is considered an important factor in crop productivity and health. Here, we investigated the bacterial diversity of seeds, roots, and rhizosphere of five sugar beet hybrids named Eduarda (ED), Koala (KO), Tibor (T), Tajfun (TF), and Cercospora-resistant (C). A culture-independent next-generation sequencing approach was used for the further investigation of seed-borne endophytes. Hybrid-associated bacteria were evaluated for their plant growth–promoting (PGP) characteristics, antagonistic activity towards Cercospora beticola and several Fusarium strains in dual culture assays, and drought and salinity tolerance. High-throughput sequencing revealed that the Proteobacteria phylum was most dominant in the seeds of all hybrids, followed by Cyanobacteria and Actinobacteriota. The predominant genus in all hybrids was Pantoea, followed by Pseudomonas, Acinetobacter, Chalicogloea, Corynebacterium, Enterobacter, Enterococcus, Glutamicibacter, Kosakonia, and Marinilactibacillus. Unique genera in the hybrids were Pleurocapsa and Arthrobacter (T), Klebsiella (TF), Apibacter (ED), and Alloscardovia (KO). The genera that were most represented in one hybrid were Weissella and Staphylococcus (TF); Streptococcus (T); Gardnerella, Prevotella, and Rothia (KO); and Gilliamella, Lactobacillus, and Snodgrassella (ED). Thirty-two bacteria out of 156 isolates from the rhizosphere, roots, and seeds were selected with respect to various plant growth–promoting activities in vitro, i.e., nitrogen fixation, phosphate solubilization, siderophore production, indole-3-acetic acid production, 1-aminocyclopropane-1-carboxylic acid deaminase activity, hydrogen cyanide production, exoenzymatic activity (amylase, protease, lipase, cellulase, xylanase, mannanases, gelatinase, and pectinase), mitigation of environmental stresses, and antifungal activity. Mixta theicola KO3-44, Providencia vermicola ED3-10, Curtobacterium pusillum ED2-6, and Bacillus subtilis KO3-18 had the highest potential to promote plant growth due to their multiple abilities (nitrogen fixation, phosphate solubilization, production of siderophores, and IAA). The best antagonistic activity towards phytopathogenic fungi was found for Bacillus velezensis C3-19, Paenibacillus polymyxa C3-36 and Bacillus halotolerans C3-16/2.1. Only four isolates B. velezensis T2-23, B. subtilis T3-4, B. velezensis ED2-2, and Bacillus halotolerans C3-16/2.1 all showed enzymatic activity, with the exception of xylanase production. B. halotolerans C3-16/2.1 exhibited the greatest tolerance to salinity, while two B. subtilis strains (C3-62 and TF2-1) grew successfully at the maximum concentration of PEG. The current study demonstrates that sugar beet–associated bacteria have a wide range of beneficial traits and are therefore highly promising for the formulation of biological control and PGP agents. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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174. New perspectives of purple starthistle (Centaurea calcitrapa) leaf extracts: phytochemical analysis, cytotoxicity and antimicrobial activity.
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Dimkić, Ivica, Petrović, Marija, Gavrilović, Milan, Gašić, Uroš, Ristivojević, Petar, Stanković, Slaviša, and Janaćković, Peđa
- Subjects
- *
ETHYL acetate , *PHENOLIC acids , *PHYTOCHEMICALS , *METHICILLIN-resistant staphylococcus aureus , *PSEUDOMONAS syringae , *CENTAUREA , *FLAVONOID glycosides , *XANTHOMONAS campestris - Abstract
Ethnobotanical and ethnopharmacological studies of many Centaurea species indicated their potential in folk medicine so far. However, investigations of different Centaurea calcitrapa L. extracts in terms of cytotoxicity and antimicrobial activity against phytopathogens are generally scarce. The phenolic profile and broad antimicrobial activity (especially towards bacterial phytopathogens) of methanol (MeOH), 70% ethanol (EtOH), ethyl-acetate (EtOAc), 50% acetone (Me2CO) and dichloromethane: methanol (DCM: MeOH, 1: 1) extracts of C. calcitrapa leaves and their potential toxicity on MRC-5 cell line were investigated for the first time. A total of 55 phenolic compounds were identified: 30 phenolic acids and their derivatives, 25 flavonoid glycosides and aglycones. This is also the first report of the presence of centaureidin, jaceidin, kaempferide, nepetin, flavonoid glycosides, phenolic acids and their esters in C. calcitrapa extracts. The best results were obtained with EtOAc extract with lowest MIC values expressed in µg/mL ranging from 13 to 25, while methicillin resistant Staphylococcus aureus was the most susceptible strain. The most susceptible phytopathogens were Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. campestris and Agrobacterium tumefaciens. The highest cytotoxicity was recorded for EtOAc and Me2CO extracts with the lowest relative and absolute IC50 values between 88 and 102 µg/mL, while EtOH extract was the least toxic with predicted relative IC50 value of 1578 µg/mL. Our results indicate that all tested extracts at concentration considered as non-toxic can be one of great importance in combat towards phytopathogenic and human pathogenic strains, as well as natural sources of antimicrobials. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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175. Effect-directed screening of Bacillus lipopeptide extracts via hyphenated high-performance thin-layer chromatography.
- Author
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Jamshidi-Aidji, Maryam, Dimkić, Ivica, Ristivojević, Petar, Stanković, Slaviša, and Morlock, Gertrud E.
- Subjects
- *
BACILLUS (Bacteria) , *MASS spectrometry , *CHROMATOGRAPHIC analysis , *BIOSURFACTANTS , *BIOLOGICAL pest control agents , *SURFACTIN - Abstract
• Efficient bioprofiling method was developed and shown for five different Bacillus strains. • Extraction of the produced lipopeptides was compared by two differing methods. • Active zones in the Bacillus strain extracts were characterized by HPTLC-ESI-HRMS. • Bioanalytical tool was demonstrated to excellently control the formed bioactive metabolites. • Biotechnological processes for therapeutics or biocontrol agents can be controlled this way. Bacillus species produce a wide array of biologically active metabolites, including nonribosomaly synthesized lipopeptides (LPs). The high-performance thin-layer chromatography (HPTLC) technique hyphenated with different bioassays and mass spectrometry was demonstrated as a valuable tool for effect-directed analysis of iturins, surfactins, fengycins and kurstakins homologues from complex mixtures of LPs. As proof of this straightforward strategy, the found surfactin and iturin A homologues were characterized and compared with reference substances. This study considered two different extraction methods for LPs produced by five Bacillus strains. The ethyl acetate extraction (Ex-1), and the acidic precipitation followed by methanol extraction (Ex-2) were investigated. Diverse enzyme inhibitions and antimicrobial potentials of LPs were analyzed, and in parallel, high-resolution mass spectra (HRMS) were online recorded from the HPTLC zones of interest. No antimicrobial effect against Gram-positive B. subtilis was evident for iturin, whereas a response was detected for surfactin. The nonpolar kurstakin compounds showed a pronounced B. subtilis antimicrobial activity in Ex-1 of almost all strains, whereas the fengycin homologues were detected in Ex-2 of SS-10.7 and SS-27.2. Iturin had also no activity against Gram-negative Aliivibrio fischeri , while again surfactin showed an enhancing luminescent activity. Contrary, kurstakin compounds caused a decrease in the luminescence in Ex-1 of all strains, particularly for SS-13.1. Both, iturin and surfactin showed a strong acetylcholinesterase (AChE) and α-glucosidase inhibition, but surfactin caused a much stronger inhibition. This was evident in all bacterial strains, except for SS-13.1 in Ex-1 and for SS-38.4 in Ex-2. Although, iturin and surfactin exhibited no DPPH˙ scavenging activity, Ex-1 of all strains contained more intense DPPH˙ scavenging compounds compared to Ex-2, and surfactin methyl esters showed a pronounced DPPH˙ activity, particularly in SS-12.6 in Ex-1. This study pointed to active metabolites of strains that can be used as therapeutics and biocontrol agents with beneficial effects on human health. The straightforward HPTLC profiling served as an excellent bioanalytical tool to control the formed bioactive metabolites. As the fermentation process is very sensitive to external influences, it could be a helpful control tool for standardization of the biotechnological processing. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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176. Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, Serbia.
- Author
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Djokić, Ivana, Knežević, Aleksandar, Savković, Željko, Ljaljević Grbić, Milica, Dimkić, Ivica, Bukvički, Danka, Gavrilović, Dragana, and Unković, Nikola
- Subjects
- *
WOOD decay , *RIVER sediments , *FIFTEENTH century , *ASCOMYCETES , *EXHIBITION space , *WOOD-decaying fungi , *ASPERGILLUS - Abstract
Two ancient wooden vessels, specifically a monoxyle (1st century BCE to 1st century CE) and shipwreck (15th to 17th century CE), were excavated in a well-preserved state east of the confluence of the old Mlava and the Danube rivers (Serbia). The vessels were found in the ground that used to be river sediment and were temporarily stored within the semi-underground exhibition space of Mammoth Park. As part of the pre-conservation investigations, the primary aim of the research presented was to characterize the culturable mycobiomes of two excavated wooden artifacts so that appropriate conservation procedures for alleviating post-excavation fungal infestation could be formulated. Utilizing culture-based methods, a total of 32 fungi from 15 genera were identified, mainly Ascomycota and to a lesser extent Mucoromycota sensu stricto. Soft-rot Ascomycota of genus Penicillium, followed by Aspergillus and Cephalotrichum species, were the most diverse of the isolated fungi. Out of a total of 38 isolates, screened on 7 biodegradation plate assays, 32 (84.21%) demonstrated at least one degradative property. Penicillium solitum had the highest deterioration potential, with a positive reaction in 5 separate plate assays. The obtained results further broaden the limited knowledge on the peculiarities of post-excavation soft-rot decay of archaeological wood and indicate the biochemical mechanisms at the root of post-excavation fungal deterioration. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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177. Effects of selected bryophyte species extracts on microorganisms
- Author
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Vujičić, Milorad, Dimkić, Ivica, Sabovlkević, Aneta, Stanković, Slavisa, and Sabovlkević, Marko
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0106 biological sciences ,Microorganism ,Botany ,Bryophyte ,General Medicine ,Biology ,010603 evolutionary biology ,01 natural sciences ,010606 plant biology & botany - Abstract
Acta Biologica Plantarum Agriensis, vol. 5. ISSN 2061-6716 (Print), 2063-6725 (Online)
178. Chironomus riparius Larval Gut Bacteriobiota and Its Potential in Microplastic Degradation.
- Author
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Janakiev, Tamara, Milošević, Đurađ, Petrović, Marija, Miljković, Jelena, Stanković, Nikola, Zdravković, Dimitrija Savić, and Dimkić, Ivica
- Subjects
- *
PLASTIC marine debris , *CHIRONOMUS riparius , *POLLUTION , *ENVIRONMENTAL indicators , *POLYVINYL chloride , *WATER pollution , *POLYAMIDES , *POLYETHYLENE - Abstract
Chironomus riparius are sediment-dwelling invertebrates in freshwater ecosystems and are used as indicators of environmental pollution. Their habitat is threatened by high levels of contaminants such as microplastics and organic matter. A promising strategy for the eco-friendly degradation of pollutants is the use of bacteria and their enzymatic activity. The aim of this study was to characterize for the first time bacteriobiota associated with the gut of C. riparius larvae from nature and laboratory samples, to compare it with sediment and food as potential sources of gut microbiota, and to assess its ability to degrade cellulose, proteins, and three different types of microplastics (polyethylene, polyvinyl chloride, and polyamide). The metabarcoding approach highlighted Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteriota as most abundant in both gut samples. Culturable microbiota analysis revealed Metabacillus idriensis, Peribacillus simplex, Neobacillus cucumis, Bacillus thuringiensis/toyonensis, and Fictibacillus phosphorivorans as five common species for nature and laboratory samples. Two P. simplex and one P. frigoritolerans isolates showed the ability for intensive growth on polyethylene, polyvinyl chloride, and polyamide. Both cellulolytic and proteolytic activity was observed for Paenibacillus xylanexedens and P. amylolyticus isolates. The characterized strains are promising candidates for the development of environmentally friendly strategies to degrade organic pollution and microplastics in freshwater ecosystems. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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179. Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres.
- Author
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Marković, Sanja, Popović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, and Stanković, Slaviša
- Subjects
- *
ERWINIA , *PHYTOPATHOGENIC bacteria , *BACTERIAL diversity , *TUBERS , *PHYTOPATHOGENIC microorganisms , *ENDOPHYTIC bacteria , *BIOLOGICAL pest control agents - Abstract
Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected field (58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present in all samples, with Proteobacteria being the most prevalent, especially in tubers without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were more represented in tubers without symptoms. However, the genus Dickeya was at the limit of detection or not detected at all. Acinetobacter was the most dominant in tubers with soft rot, while Pseudomonas and Enterobacter were the most abundant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with starch-degradable features were almost exclusively present in soft rotted tubers and their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confirmed biocontrol potential. This study indicates that the soft rot maceration process arises due to complex interactions between plant pathogens and other endophytic bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
180. An Insight into an Olive Scab on the "Istrska Belica" Variety: Host‐Pathogen Interactions and Phyllosphere Mycobiome.
- Author
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Hladnik, Matjaž, Unković, Nikola, Janakiev, Tamara, Grbić, Milica Ljaljević, Arbeiter, Alenka Baruca, Stanković, Slaviša, Janaćković, Peđa, Gavrilović, Milan, Rančić, Dragana, Bandelj, Dunja, and Dimkić, Ivica
- Subjects
- *
OLIVE , *LEAF spots , *BIOLOGICAL pest control agents , *PLANT breeding , *AGRICULTURE , *PLANT defenses , *PLANT-pathogen relationships , *OLIVE leaves - Abstract
The olive tree is one of the most important agricultural plants, affected by several pests and diseases that cause a severe decline in health status leading to crop losses. Olive leaf spot disease caused by the fungus Venturia oleaginea can result in complete tree defoliation and consequently lower yield. The aim of the study was to obtain new knowledge related to plant–pathogen interaction, reveal mechanisms of plant defense against the pathogen, and characterize fungal phyllosphere communities on infected and symptomless leaves that could contribute to the development of new plant breeding strategies and identification of novel biocontrol agents. The highly susceptible olive variety "Istrska Belica"' was selected for a detailed evaluation. Microscopy analyses led to the observation of raphides in the mesophyll and parenchyma cells of infected leaves and gave new insight into the complex V. oleaginea pathogenesis. Culturable and total phyllosphere mycobiota, obtained via metabarcoding approach, highlighted Didymella, Aureobasidium, Cladosporium, and Alternaria species as overlapping between infected and symptomless leaves. Only Venturia and Erythrobasidium in infected and Cladosporium in symptomless samples with higher abundance showed statistically significant differences. Based on the ecological role of identified taxa, it can be suggested that Cladosporium species might have potential antagonistic effects on V. oleaginea. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
181. Bacteriome composition analysis of selected mineral water occurrences in Serbia.
- Author
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Šaraba, Vladimir, Dragišić, Veselin, Janakiev, Tamara, Obradović, Vesna, Ćopić, Milica, Knežević, Branislav, and Dimkić, Ivica
- Subjects
- *
MINERAL waters , *MINERALS in water , *MINERAL analysis , *WATER pollution , *COLIFORMS , *SULFATE-reducing bacteria - Abstract
Bacterial metabarcoding analysis by 16S rDNA of five occurrences of mineral waters in Serbia (Torda, Slankamen Banja, Lomnički Kiseljak, Velika Vrbnica and Obrenovačka Banja) indicated the presence of a high percentage of the Proteobacteria phylum, followed by the Bacteroidetes phylum. The families Rhodobacteraceae, Burkholderiaceae, Pseudomonadaceae, Methylophilaceae and Moraxellaceae were the most dominant in the bacterial flora of the selected occurrences, whereas the most represented genera were Acinetobacter, Pseudorhodobacter, Pseudomonas, Limnohabitans, Massilia, Limnobacter and Methylotenera. The presence of coliform bacteria was not detected. Alpha diversity analysis revealed that Slankamen Banja and Lomnički Kiseljak were the richest of the selected occurrences, while the mineral waters of Torda, Velika Vrbnica and Obrenovačka Banja were characterized by similar diversity of bacterial communities determined by beta diversity analysis. Physical-chemical analysis revealed the value of total dissolved solids above 1 g/L, as well as elevated concentrations of some metals and non-metals. The research concluded that specific bacteria contribute to the development of biocorrosion and biofouling processes of water intake facilities. In addition, some of these bacteria might be potential indicators of the organic sources of pollution and/or biotechnological natural remediators in the treatment of contaminated waters. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
182. Gamma-Ray-Induced Structural Transformation of GQDs towards the Improvement of Their Optical Properties, Monitoring of Selected Toxic Compounds, and Photo-Induced Effects on Bacterial Strains
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Sladjana Dorontic, Aurelio Bonasera, Michelangelo Scopelliti, Olivera Markovic, Danica Bajuk Bogdanović, Gabriele Ciasca, Sabrina Romanò, Ivica Dimkić, Milica Budimir, Dragana Marinković, Svetlana Jovanovic, Dorontic, Sladjana, Bonasera, Aurelio, Scopelliti, Michelangelo, Markovic, Olivera, Bajuk Bogdanović, Danica, Ciasca, Gabriele, Romanò, Sabrina, Dimkić, Ivica, Budimir, Milica, Marinković, Dragana, and Jovanovic, Svetlana
- Subjects
graphene quantum dots ,3-amino-1,2,4-triazole ,gamma-irradiation ,General Chemical Engineering ,N-doping ,photoluminescence ,carbofuran ,detection ,antibacterial effects ,General Materials Science ,Settore CHIM/02 - Chimica Fisica - Abstract
Structural modification of different carbon-based nanomaterials is often necessary to improve their morphology and optical properties, particularly the incorporation of N-atoms in graphene quantum dots (GQDs). Here, a clean, simple, one-step, and eco-friendly method for N-doping of GQDs using gamma irradiation is reported. GQDs were irradiated in the presence of the different ethylenediamine (EDA) amounts (1 g, 5 g, and 10 g) and the highest % of N was detected in the presence of 10 g. N-doped GQDs emitted strong, blue photoluminescence (PL). Photoluminescence quantum yield was increased from 1.45, as obtained for non-irradiated dots, to 7.24% for those irradiated in the presence of 1 g of EDA. Modified GQDs were investigated as a PL probe for the detection of insecticide Carbofuran (2,2-Dimethyl-2,3-dihydro-1-benzofuran-7-yl methylcarbamate) and herbicide Amitrole (3-amino-1,2,4-triazole). The limit of detection was 5.4 μmol L−1 for Carbofuran. For the first time, Amitrole was detected by GQDs in a turn-off/turn-on mechanism using Pd(II) ions as a quenching agent. First, Pd(II) ions were quenched (turn-off) PL of GQDs, while after Amitrole addition, PL was recovered linearly with Amitrole concentration (turn-on). LOD was 2.03 μmol L−1. These results suggest that modified GQDs can be used as an efficient new material for Carbofuran and Amitrole detection. Furthermore, the phototoxicity of dots was investigated on both Gram-positive and Gram-negative bacterial strains. When bacterial cells were exposed to different GQD concentrations and illuminated with light of 470 nm wavelength, the toxic effects were not observed.
- Published
- 2022
183. Toxic effects of a cyanobacterial strain on Chironomus riparius larvae in a multistress environment.
- Author
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Stanković, Nikola, Jovanović, Boris, Kokić, Ivana Kostić, Piperac, Milica Stojković, Simeunović, Jelica, Jakimov, Dimitar, Dimkić, Ivica, and Milošević, Djuradj
- Subjects
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CHIRONOMUS riparius , *CYANOBACTERIAL toxins , *POISONS , *ACUTE toxicity testing , *LARVAE , *SOMATIC cells - Abstract
Cyanobacteria and their toxic metabolites present a global threat to water habitats, but their impact on aquatic organisms in a multistress environment has been poorly investigated. Here we present the results of a survey on the effects of the toxic cyanobacterial strain Trichormus variabilis (heterotypic synonym Anabaena variabilis), and its toxic metabolite, cyanotoxin microcystin-LR, on Chironomus riparius larvae in a multistress environment. An environmentally relevant concentration of microcystin-LR (0.01 mg/L) caused an increase in larvae mortality in an acute toxicity test, which became greater in the presence of environmental stressors (NO 3 −, NH4+, PO 4 3− and Cd2+), pointing to an additive effect of these agents. Chronic exposure of C. riparius larvae to the microcystin-LR producing strain of T. variabilis in a multistress environment led to a reduction in the larval mass and hemoglobin concentration, and it induced DNA damage in larval somatic cells. The results revealed the additive effect of microcystin-LR in combination with all three tested stressors (NO 3 −, NH4+, PO 4 3−), and the deleterious effect of chronic exposure of C. riparius larvae to the microcystin-LR producing T. variabilis in a multistress environment. However, the present study further emphasizes the importance of investigating interactions between stressors and cyanotoxins, and their effect on aquatic organisms. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
184. Genotipizacija, rezistencija na antibiotike i ispitivanje faktora virulencije kliničkih izolata Pseudomonas aeruginosa
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Milojković, Marko, Dimkić, Ivica, Božić, Dragana, and Stanković, Slaviša
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serotipizacija ,formiranje biofilma ,β-lactamases ,genetic patterns ,genotipski obrasci ,serotyping ,RAPD PCR ,antibiotics susceptibility ,Pseudomonas aeruginosa ,biofilm formation ,pigmentacija ,pigmentation ,osetljivost na antibiotike ,β-laktamaze - Abstract
Utvrđivanje potencijalnih korelacija, između fenotipskih karakteristika (osetljivost na različite klase antibiotika, ekspresija različitih klasa β-laktamaza, učestalost serotipova, proizvodnja pigmenata i stvaranje biofilma) i genotipskih obrazaca kliničkih izolata Pseudomonas aeruginosa različitog porekla, predstavljalo je glavni cilj ovog rada. Tokom dve faze istraživanja, ispitano je 147 kliničkih izolata, od kojih je najveći procenat dobijen iz briseva rana (66%) i urino-kultura (10,9%). Polivalentne serogrupe PMA i PMF, kao i monovalentni serotipovi P1, P6 i P11 bili su najzastupljeniji, dok je najveći procenat izolata bio očekivano netipabilan. Istovremena produkcija oba pigmenta karakterisala je najveći broj izolata, dok su oni sa serotipovima P1, P6 i P11 produkovali više pioverdina nego piocijanina. Sposobnost formiranja biofilma imalo je 77,66% kliničkih izolata, a najizraženija je utvrđena za izolate iz urinokultura i sputuma. Najveći stepen osetljivosti utvrđen je za kolistin, aztreonam, karbapeneme i piperacilin/tazobaktam, dok je izraženija otpornost prikazana prema aminoglikozidima i fluorohinolonima. Višestruku otpornost na tri ili više klasa antibiotika je pokazalo 30,9% izolata iz druge faze istraživanjaje, a skoro polovina je eksprimirala β-laktamaze proširenog spektra, karbapenemaze i metalo-β-laktamaze. RAPD272 PCR analiza pokazala se kao najbolja tehnika koja je izdvojila najveći broj klastera sa ujednačenim obrascima, ističući izraženu heterogenost izolata iste vrste. Korelacija između genetičkih obrazaca i fenotipskih karakteristika nije ustanovljena, ukazujući na potrebu za novim molekularnim metodama u ovom delu Srbije u cilju otkrivanja potencijalnih veza među njima i na izraženu rezistenciju koja se poslednjih godina uvećava. Determining potential correlations between phenotypic characteristics (sensitivity to different classes of antibiotics, expression of different β-lactamases classes, frequency of serotypes, pigment and biofilm production) and genotypic patterns of clinical isolates of Pseudomonas aeruginosa from different origins was the main goal of this study. Study itself was splitted into two phases, a total of 147 clinical isolates were examined, out of which the largest percentage were from wound swabs (66%) and urine cultures (10.9%). Polyvalent serogroups PMA and PMF, as well as monovalent serotypes P1, P6 and P11 were the most represented, while the highest percentage of isolates was atypical. Simultaneous production of both pigments characterized the most of isolates, while those with serotypes P1, P6 and P11 produced more pyoverdine than pyocyanin. Even 77.66% of clinical isolates had the ability to form a biofilm, and the most pronounced was found for isolates from urine cultures and sputum. The highest susceptibility was found for colistin, aztreonam, carbapenems and piperacillin/tazobactam, while more pronounced resistance was shown onto aminoglycosides and fluoroquinolones. Multiple resistances, up to three or more antibiotics classes, were shown by 30.9% of isolates from the second study phase, and almost half of them expressed broad-spectrum β-lactamases, carbapenemases and metallo-β-lactamases. RAPD272 PCR analysis proved to be the best technique that singled out the largest number of clusters with uniform patterns, emphasizing the pronounced heterogeneity of isolates of the same species. The correlation between genetic patterns and phenotypic characteristics has not been established, indicating the need for new molecular methods in this part of Serbia to discover potential links between them and on the pronounced resistance, which has increased in recent years.
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- 2021
185. Microorganisms - biohydrogeological indicators of select mineral water occurrences in Serbia
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Šaraba, Vladimir, Veselin Dragišić, Ivica Dimkić, Vladimir Živanović, Jana Štrbački, Marina Ćuk Đurović, Tamara Janakiev, Tanja Petrović Pantić, Dragišić, Veselin, Dimkić, Ivica, Živanović, Vladimir, Stojković, Jana, and Ćuk, Marina
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biochemical encrustation and biofouling) ,health risk ,biohydrogeology ,makroskopska i optička ispitivanja ,biohidrogeologija ,hidrogeohemijsko modeliranje ,zdravstveni rizik ,metabarkoding analize mikrobioma ,SEM analyses ,BART analize ,starenje bunara (biokorozija ,BART analyses ,rendgenska difrakcija praha ,metabarcoding of microbiomes ,physical and chemical analyses ,SEM analize ,hydrogeochemical modelling ,X-ray powder diffraction ,macroscopic and optical assessment ,biohydrogeology, SEM analyses, macroscopic and optical assessment, X-ray powder diffraction, hydrogeochemical modelling, BART analyses, metabarcoding of microbiomes, physical and chemical analyses, well aging (biocorrosion, biochemical encrustation and biofouling), health risk ,well aging (biocorrosion ,biohemijska inkrustacija i biozarastanje) ,fizičko-hemijske analize ,biohidrogeologija, SEM analize, makroskopska i optička ispitivanja, rendgenska difrakcija praha, hidrogeohemijsko modeliranje, BART analize, metabarkoding analize mikrobioma, fizičko-hemijske analize, starenje bunara (biokorozija, biohemijska inkrustacija i biozarastanje), zdravstveni rizik - Abstract
M70 M70 Sa namerom definisanja hidrogeološke uloge i značaja mikrobiološkog diverziteta odabranih pojava mineralnih voda Srbije, primenom sistema biodetektora, dokazano je prisustvo gvožđevitih, sulfato-redukujućih, sluz-produkujućih, heterotrofnih aerobnih i denitrifikacionih bakterija, na osnovu čega je proračunat rizik od razvoja procesa biokorozije i biohemijske inkrustacije, te zdravstveni rizik. Uz procenu rizika, izvedene su analize skenirajuće elektronske mikroskopije biofilmova i hidrogeohemijsko modeliranje, te makroskopska, otpička i difrakciona ispitivanja naslaga inkrustacije, dok je fizičko-hemijskim analizama utvrđena vrednost ukupne mineralizacije iznad 1 g/L, kao i povišeni sadržaji odgovarajućih metala i nemetala. Metabarkoding analizama mikrobioma mineralnih voda dokazano je prisustvo razdela Proteobacteria, Bacteroidetes, Patescibacteria, Chlorflexi, Firmicutes, Spirochaetes i Acidobacteria u većem procentu. Pseudomonas, Limnobacter i Rheinheimera su bili najzastupljeniji rodovi u uzorku mineralnih voda Torde, Acinetobacter i Massilia u uzorku iz Obrenovačke Banje, Maritimimonas, Phreatobacter, Planktosalinus, Acinetobacter, Geminocystis i Sulfuritalea u uzorku iz Lomnice, Pseudorhodobacter i Limnohabitans u mineralnim vodama Velike Vrbnice, dok su u uzorku iz Slankamen Banje dominirale zelene sumporne bakterije, Methylotenera, Flavobacterium, Melioribacter i Dechloromonas. Realizovanim istraživanjima zaključeno je da odgovarajući predstavnici mikroorganizama utiču na starenje bunara i definišu ekološki status vodnih resursa, dok s druge strane pojedini rodovi poseduju biotehnološki potencijal u tretmanu remedijacije kontaminiranih voda, zbog čega postoji neophodnost implementacije biohidrogeologije u okviru standardne metodike hidrogeoloških istraživanja. The presence of iron related, sulphate reducing, slime producing, heterotrophic aerobic and denitrifying bacteria was proved applying biodetectors, in order to define the hydrogeological role and importance of the microbiological diversity of select occurrences of mineral waters in Serbia. Then the biocorrosion, biochemical encrustation and health risks were assessed. The research included scanning electron microscopy of biofilms, hydrogeochemical modelling and macroscopic, optical and diffraction examination of the encrustations. Physical and chemical analyses indicated total dissolved salts of more than 1 g/L and elevated concentrations of certain metals and non-metals. Metabarcoding of the microbiomes of the mineral waters revealed the presence of a high percentage of the Proteobacteria, Bacteroidetes, Patescibacteria, Chlorflexi, Firmicutes, Spirochaetes and Acidobacteria divisions. Pseudomonas, Limnobacter and Rheinheimera were the most representative genera in the mineral water sample from Torda, Acinetobacter and Massilia in the sample collected in Obrenovačka Banja, Maritimimonas, Phreatobacter, Planktosalinus, Acinetobacter, Geminocystis and Sulfuritalea in the sample from Lomnica, and Pseudorhodobacter and Limnohabitans in the sample from Velika Vrbnica, while in the sample from Slankamen Banja was dominated by green sulphur bacteria, Methylotenera, Flavobacterium, Melioribacter and Dechloromonas. The research concludes that the respective microorganisms cause water well aging and define the ecological status of the water resources, while, on the other hand, some of the genera possess the biotechnological potential for treating and remediating contaminated waters, which is a compelling reason for implementing biohydrogeology in the standard hydrogeological research methodology.
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- 2021
186. Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive
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Janakiev, Tamara, Berić, Tanja, Dimkić, Ivica, Stanković, Slaviša, Opsenica, Dejan, and Unković, Nikola D.
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fenazini ,šljiva ,Bacillus thuringiensis ,biological control ,Monilinia laxa ,phenazines ,Pseudomonas synxantha ,antagonizam ,diverzitet mikroorganizama ,antagonism ,filosfera ,NGS ,microbial diversity ,quorum quenching ,phyllosphere ,plum ,biološka kontrola - Abstract
Šljiva (Prunus domestica L.) ima izuzetan značaj kao voćarska kultura u Srbiji i tradicionalno se prerađuje u rakiju šljivovicu. S obzirom da je godišnji prinos šljive ugrožen različitim bolestima, naročito izazivačima bakterioznog raka i mrke truleži neophodno je naći efikasne agense za njihovo suzbijanje, a naročito alternativu upotrebi hemijskih pesticida. U ovom radu je prvi put analiziran mikrobiom filosfere četiri sorte šljive (Čačanska lepotica, Čačanska rodna, Požegača i Ranka), obuhvatajući ranu (maj) i kasnu fenološku fazu (juli) razvića ploda. Indeksi alfa i beta diverziteta su pokazali da su zajednice bile heterogene i da je njihov sastav zavisio od sezone uzorkovanja i sorti šljive. U ukupnoj bakterijskoj zajednici je metagenomskim pristupom utvrđeno da su predstavnici razdela Proteobacteria bili najdominantniji, a najzastupljenije vrste su bile iz rodova: Methylobacterium, Sphingomonas i Hymenobacter. Tradicionalnim kultivabilnim pristupom i analizom sekvenci gena za 16S rRNK identifikovane su Pseudomonas syringae i Pseudomonas graminis kao najdominantnije vrste. Analiza mikobioma je ukazala na dominatnu zastupljenost predstavnika razdela Ascomycota. Vrste iz rodova Aureobasidium i Cryptococcus su bile najučestalije u maju, dok su u julu predstavnici različitih rodova dominirali na sortama šljive (Cryptococcus, Metschnikowia, Fusarium i Hanseniaspora). U okviru kolekcije kultivabilnih gljiva, identifikovano je 11 izolata Monilinia laxa na osnovu filogenetske analize sekvenci ITS regiona. Odabrani izolati M. laxa su prouzrokovali tipične simptome na ubranim plodovima sorti Požegača i Čačanska rodna, a testiranjem potencijalnih bakterijskih patogena su izdvojeni izolati P. syringae ČL2/2 i P7/16_2, koji su izazivali nekroze na ubranim listovima šljive svih ispitivanih sorti. Analizom antagonističkog potencijala kolekcije bakterija i kvasaca poreklom sa sve četiri sorte je izdvojen izolat Bacillus thuringiensis R3/3 sa snažnom antibakterijskom aktivnošću protiv autohtonih i referentnih fitopatogena... Plum (Prunus domestica L.) has a great importance as a fruit crop in Serbia and it is traditionally processed into slivovitz brandy. Considering that the annual plum yield is significantly affected by various pathogens, especially those causing bacterial canker and brown rot, it is necessary to find effective agents for their control, and in particular, an alternative to the chemical pesticides. In this research, microbiome of four plum cultivars phyllosphere (Čačanska lepotica, Čačanska rodna, Požegača i Ranka) was analyzed for the first time, including the early (May) and late phenological stages (July) of fruit development. Alpha and beta diversity indices had shown that the communities were heterogenous and that their composition depended on sampling season and plum cultivars. The metagenomic approach revealed that the representatives of the phylum Proteobacteria were the most dominant in the total bacterial community, and that the most represented species were from the genera: Methylobacterium, Sphingomonas and Hymenobacter. Traditional cultivable approach and 16S rRNA sequence analyses identified Pseudomonas syringae i Pseudomonas graminis as two most dominant species. Mycobiome analysis revealed the dominance of the phylum Ascomycota representatives. Aureobasidium and Cryptococcus species were the most abundant in May, while in July representatives of different genera dominated the plum cultivars (Cryptococcus, Metschnikowia, Fusarium and Hanseniaspora). From the collection of cultivable fungi, eleven Monilinia laxa isolates were identified based on phylogenetic analysis of the ITS region sequences. The selected M. laxa isolates caused typical symptoms on the harvested fruits of the Požegača and Čačanska rodna cultivars, and the testing of potential bacterial pathogens revealed P. syringae ČL2/2 and P7/16_2 isolates, which caused necrosis on the harvested plum leaves of all tested cultivars. Antagonistic potential analysis of bacterial and yeast isolates from all four cultivars singled out Bacillus thuringiensis R3/3 with strong antibacterial activity against native and reference phytopathogens...
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- 2019
187. Chemical analysis of secondary metabolites of selected plant species of the genus Euphorbia
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Krstić, Gordana, Tešević, Vele, Aljančić, Ivana, Todorović, Nina, Vujisić, Ljubodrag, and Dimkić, Ivica
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Ingenani ,Metabolomika ,Jatrophanes ,Euphorbia palustris ,Euphorbia lucida ,Tiglianes ,Multidrug resistance (MDR) ,Višestruka reverzija na lekove (MDR) ,P-glycoprotein ,P-glikoprotein ,Tiglijani ,Ingenanes ,Jatrofani ,Euphorbia ,Metabolomics ,Euphorbia nicaeensis - Abstract
Predmet ove doktorske disertacije je proučavanje sekundarnih metabolita odabranih biljnih vrsta roda Euphorbia koje nastanjuju naše prostore. Za ispitivanje su odabrane Euphorbia palustris L. (barska mlečika), Eupohorbia lucida Waldst. & Kit. (visoka mlečika) i Euphorbia nicaeensis All (uskolisna mlečika). Biljne vrste roda Euphorbia poznate su od davnina i našle su svoju praktičnu primenu u tradicionalnoj i savremenoj medicini. Za sve predstavnike ovog roda karakteristično je postojanje biljnog soka (lateks) koji se skadišti u posebnim ćelijama koje se nalaze u stablu i listovima biljaka. Lateks predstavlja vodenu emulziju smeše terpena, fenolnih jedinjenja, cerebrozida, glicerola i drugih jedinjenja koja ima zaštitno dejstvo za biljku. Najzastupljenija jedinjenja u lateksu pripadaju klasi terpena i to diterpenima i triterpenima... The subject of this doctoral dissertation is the study of secondary metabolites of selected plant species of the genus Euphorbia that grow in our region. Euphorbia palustris L. (swamp spurge), Euphorbia lucida Waldst. & Kit. (shining spurge) and Euphorbia nicaeensis All. (narrow-leaved spurge) were selected for the research. Plant species of the genus Euphorbia have been known since ancient times and have found practical applications in traditional and modern medicine. All the plants of this genus are characterized by the existence of a milky sap (latex) which is stored in specialized cells found in the stem and leaves of the plants. Latex is an aqueous emulsion of a mixture of terpenes, phenolic compounds, cerebrosides, glycerols and other compounds that has a protective effect on the plant. Terpenes are the major components of the latex, namely diterpenes and triterpenes...
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- 2019
188. Identifikacija i karakterizacija Bacillus spp. izolata sa potencijalom za biokontrolu fungalnih patogena šampinjona
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Stanojević, Olja, Berić, Tanja, Milijašević-Marčić, Svetlana, Potočnik, Ivana, Stanković, Slaviša, and Dimkić, Ivica
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Trichoderma ,green mould ,lipopeptidi ,Bacillus ,Agaricus bisporus ,zelena plesan ,antagonizam ,biološko suzbijanje ,lipopeptides ,antagonism ,suva trulež ,biocontrol ,Lecanicillium fungicola ,dry bubble - Abstract
Najštetniji patogeni šampinjona čija pojava u gajilištima izaziva velike gubitke u prinosu su gljive Trichoderma spp., prouzrokovači zelene plesni i Lecanicillium fungicola, prouzrokovač suve truleži šampinjona... The most devastating fungal pathogens of the white button mushroom that induced the greatest yield losses are Trichoderma spp. and Lecanicillium fungicola, causal agents of green mould and dry bubble disease...
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- 2019
189. Antimicrobial and antitumor potential of the secretions and morphological characterization of the glandular structures of the pygidial glands of selected species of ground beetles (Coleoptera: Carabidae)
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Nenadić, Marija Z., Ćurčić, Srećko B., Ćirić, Ana, Soković, Marina, Pantelić, Dejan, and Dimkić, Ivica
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mikrodiluciona metoda ,NLM ,antimicrobial activity ,human pathogens ,antimikrobna aktivnost ,citotoksični efekat ,antitumor potential ,sulforhodamine B assay ,sulforodamin B esej ,microdilution method ,SEM ,antitumorski potencijal ,cytotoxic effect ,Carabidae ,humani patogeni ,guano ,pigidijalne žlezde ,pygidial glands ,TPEF - Abstract
Ispitivanje pigidijalnog žlezdanog kompleksa nalazi značajno mesto u karabidologiji, prevashodno sa stanovišta morfologije i taksonomije, ali u novije vreme i ostalih aspekata koji se mogu odnositi na potencijalnu upotrebu primarnih produkata ovih žlezda u biomedicinskim istraživanjima. Ovaj kompleks predstavlja sistem egzokrinih žlezda koji se sastoji od sekretornih lobusa izgrađenih od sekretornih ćelija, gde se sekreti sintetišu, zatim radijalnih sabirnih kanala, glavnog sabirnog kanala, rezervoara, gde se sekreti skladište, i eferentnog kanala, čija je uloga transport sekreta iz rezervoara u spoljašnju sredinu. Primarna funkcija sekreta pigidijalnih žlezda jeste odbrana trčuljaka od potencijalnih predatora iz prirode. U hemijski sastav ovih sekreta ulazi čitav niz jedinjenja koja se uglavnom ubrajaju u aldehide, ketone i organske kiseline. U poslednje vreme postoji interesovanje za istraživanje antimikrobnog i antitumorskog potencijala navedenih smeša sekreta, s obzirom da je prethodno dokazan antimikrobni i antitumorski potencijal pojedinačnih jedinjenja koja se nalaze u njihovom sastavu. Ciljevi ove doktorske disertacije su usmereni ka ispitivanju antimikrobnog i antitumorskog potencijala sekreta pigidijalnih žlezda odabranih vrsta trčuljaka, što može imati značaj u bazičnim biomedicinskim istraživanjima, kao i ka pronalaženju pozadine ovakvih bioloških aktivnosti u ekološkoj funkciji kod ovih insekata, koja bi podobnije bila približena detaljnijom opservacijom pigidijalnih žlezdanih struktura sa morfo-anatomskog aspekta, primenom elektronske i nelinearne mikroskopije, što bi doprinelo i boljem razumevanju fiziologije ovog sistema. U prvom delu doktorske disertacije koji obuhvata biomedicinski aspekt, posmatrana je po prvi put antimikrobna aktivnost sekreta pigidijalnih žlezda 6 odabranih vrsta trčuljaka na humane patogene (osam laboratorijskih i kliničkih bakterijskih sojeva i osam patogenih mikromiceta), kao i citotoksičnost sekreta 4 odabrane vrste trčuljaka na netumorske (primarne ćelije jetre izolovane iz organizma svinje) i humane tumorske ćelijske linije (nesitnoćelijski karcinom pluća, vii cervikalni karcinom, adenokarcinom dojke i hepatocelularni karcinom)...
- Published
- 2019
190. Ispitivanje hemijskog sastava sekundarnih metabolita odabranih biljnih vrsta roda Euphorbia
- Author
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Krstić, Gordana B., Tešević, Vele, Aljančić, Ivana, Todorović, Nina, Vujisić, Ljubodrag V., and Dimkić, Ivica
- Subjects
Ingenani ,Metabolomika ,Jatrophanes ,Euphorbia palustris ,Euphorbia lucida ,Tiglianes ,Multidrug resistance (MDR) ,Višestruka reverzija na lekove (MDR) ,P-glycoprotein ,P-glikoprotein ,Tiglijani ,Ingenanes ,Jatrofani ,Euphorbia ,Metabolomics ,Euphorbia nicaeensis - Abstract
Predmet ove doktorske disertacije je proučavanje sekundarnih metabolita odabranih biljnih vrsta roda Euphorbia koje nastanjuju naše prostore. Za ispitivanje su odabrane Euphorbia palustris L. (barska mlečika), Eupohorbia lucida Waldst. & Kit. (visoka mlečika) i Euphorbia nicaeensis All (uskolisna mlečika). Biljne vrste roda Euphorbia poznate su od davnina i našle su svoju praktičnu primenu u tradicionalnoj i savremenoj medicini. Za sve predstavnike ovog roda karakteristično je postojanje biljnog soka (lateks) koji se skadišti u posebnim ćelijama koje se nalaze u stablu i listovima biljaka. Lateks predstavlja vodenu emulziju smeše terpena, fenolnih jedinjenja, cerebrozida, glicerola i drugih jedinjenja koja ima zaštitno dejstvo za biljku. Najzastupljenija jedinjenja u lateksu pripadaju klasi terpena i to diterpenima i triterpenima... The subject of this doctoral dissertation is the study of secondary metabolites of selected plant species of the genus Euphorbia that grow in our region. Euphorbia palustris L. (swamp spurge), Euphorbia lucida Waldst. & Kit. (shining spurge) and Euphorbia nicaeensis All. (narrow-leaved spurge) were selected for the research. Plant species of the genus Euphorbia have been known since ancient times and have found practical applications in traditional and modern medicine. All the plants of this genus are characterized by the existence of a milky sap (latex) which is stored in specialized cells found in the stem and leaves of the plants. Latex is an aqueous emulsion of a mixture of terpenes, phenolic compounds, cerebrosides, glycerols and other compounds that has a protective effect on the plant. Terpenes are the major components of the latex, namely diterpenes and triterpenes...
- Published
- 2019
191. Uncovering the Role of Autochthonous Deteriogenic Biofilm Community: Rožanec Mithraeum Monument (Slovenia).
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Ljaljević Grbić M, Dimkić I, Janakiev T, Kosel J, Tavzes Č, Popović S, Knežević A, Legan L, Retko K, Ropret P, and Unković N
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- Slovenia, Ascomycota physiology, Mycobiome, Biofilms, Lichens microbiology, Lichens physiology, Calcium Carbonate
- Abstract
The primary purpose of the study, as part of the planned conservation work, was to uncover all aspects of autochthonous biofilm pertaining to the formation of numerous deterioration symptoms occurring on the limestone Rožanec Mithraeum monument in Slovenia. Using state-of-the-art sequencing technologies combining mycobiome data with observations made via numerous light and spectroscopic (FTIR and Raman) microscopy analyses pointed out to epilithic lichen Gyalecta jenensis and its photobiont, carotenoid-rich Trentepohlia aurea, as the origin of salmon-hued pigmented alterations of limestone surface. Furthermore, the development of the main deterioration symptom on the monument, i.e., biopitting, was instigated by the formation of typical endolithic thalli and ascomata of representative Verrucariaceae family (Verrucaria sp.) in conjunction with the oxalic acid-mediated dissolution of limestone. The domination of lichenized fungi, as the main deterioration agents, both on the relief and surrounding limestone, was additionally supported by the high relative abundance of lichenized and symbiotroph groups in FUNGuild analysis. Obtained results not only upgraded knowledge of this frequently occurring but often overlooked group of extremophilic stone heritage deteriogens but also provided a necessary groundwork for the development of efficient biocontrol formulation applicable in situ for the preservation of similarly affected limestone monuments., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
192. Antibacterial Activities of Agaricus bisporus Extracts and Their Synergistic Effects with the Antistaphylococcal Drug AFN-1252.
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Jankov M, Léguillier V, Gašić U, Anba-Mondoloni J, Ristivojević MK, Radoičić A, Dimkić I, Ristivojević P, and Vidic J
- Abstract
Agaricus bisporus , commonly known as the button mushroom, has attracted attention for its biological properties, including antimicrobial activities. Here, we evaluated the efficacy of ethanolic and acetonic extracts from white and brown A. bisporus against different bacterial strains, including antibiotic-resistant strains. Bioautography and principal component analysis identified the most active antibacterial compounds for each of the tested bacteria and indicated the main markers responsible for the strain-specific effects. In addition, the mushroom extracts demonstrated a synergistic impact when combined with the antistaphylococcal antibiotic AFN-1252.
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- 2024
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193. Potential application of green extracts rich in phenolics for innovative functional foods: natural deep eutectic solvents as media for isolation of biocompounds from berries.
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Lazović MČ, Jović MD, Petrović M, Dimkić IZ, Gašić UM, Milojković Opsenica DM, Ristivojević PM, and Trifković JĐ
- Subjects
- Antioxidants pharmacology, Antioxidants chemistry, Blueberry Plants chemistry, Flavonoids pharmacology, Flavonoids chemistry, Flavonoids isolation & purification, Coriandrum chemistry, Fruit chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Phenols chemistry, Phenols pharmacology, Phenols isolation & purification, Functional Food, Deep Eutectic Solvents chemistry
- Abstract
The health-promoting effects of berries have attracted attention due to the possible application of their extracts as functional ingredients in food products. Natural deep eutectic solvents (NADESs) are a new generation of environmentally friendly solvents for the extraction of natural products, and they are green alternatives to organic solvents, and they can improve the solubility, stability, and bioavailability of isolated biocompounds. In this study, an efficient eco-friendly method was used for the extraction of phenolic compounds from different berries: chokeberries, blueberries, and black goji berries with a range of eutectic solvents consisting of hydrogen bond acceptors (HBAs) such as choline chloride, L-proline, L-glycine, and L-lysine and hydrogen bond donors (HBDs) such as malic, citric, tartaric, lactic and succinic acids, glucose and glycerol. The obtained results indicated the ability of NADESs towards selective extraction of phenolics; the eutectic system choline chloride : malic acid showed selective extraction of anthocyanins, while choline chloride : glycerol and choline chloride : urea showed selectivity towards flavonoids and phenolic acids. The methodology for screening of the NADES extraction performance, which included chromatographic profiling via high-performance thin layer chromatography combined with chemometrics and spectrophotometric essays, allowed effective assessment of optimal eutectic solvents for isolation of different groups of phenolics. Great antioxidant and antimicrobial activities of extracts, along with the green nature of eutectic solvents, enable NADES berry extracts to be used as "green-labelled" functional foods or ingredients.
- Published
- 2024
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- View/download PDF
194. Combined effect of cover crops and bio-fertilizer on sustainable popcorn maize production.
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Dragičević V, Simić M, Dolijanović Ž, Đorđević S, Stoiljković M, Dimkić I, and Brankov M
- Abstract
Cover crops play an important role in low-input cropping systems, increasing the use of agro-ecosystem services. Due to the lack of information about the impact of cover crops and bio-fertilizers on popcorn maize ( Zea mays everta Sturt.) growth and yield quality, especially the popping volume and nutritive quality, such as concentrations of protein and mineral elements, this research aimed to provide essential information. The interrelation between popcorn maize productivity and quality with important groups of soil microorganisms presents additional novelty. The results demonstrated that field pea is a beneficial cover crop, especially when combined with a bio-fertilizer, supporting the accumulation of maize biomass, chlorophyll, yield potential, and the concentrations of protein, Ca, Mg, Fe, and Zn. In addition, field pea residues promoted N-fixing bacteria, and the number of total microorganisms, especially actinomycetes and decomposing bacteria, which could promote nutrient uptake and grain quality. Residues of cover crop mixtures, common vetch + winter oats and field pea + winter oats, promoted the total number of microorganisms in the soil, and up to the end of vegetation, a greater number of decomposition and ammonification microorganisms were found, especially when the bio-fertilizer was applied, which consequently could support greater maize biomass. Popping volume, as a main trait of popcorn maize, had the highest value in the common vetch + winter oats variant, supporting again the statement that quality traits could be enhanced in sustainable production. Unlike living cover crops, mulch mainly affected soil microbial communities and promoted the development of actinomycetes and cellulolytic microorganisms during the growing season. The results of this research could contribute to the development of sustainable popcorn maize production for improved grain quality. They could also serve as a basis for isolating beneficial soil microorganisms to develop new bio-fertilizers that could improve maize production in synergy with cover crops., Competing Interests: SD was employed by company Agrounik d.o.o. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Dragičević, Simić, Dolijanović, Đorđević, Stoiljković, Dimkić and Brankov.)
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- 2024
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195. Insights into Endophytic and Rhizospheric Bacteria of Five Sugar Beet Hybrids in Terms of Their Diversity, Plant-Growth Promoting, and Biocontrol Properties.
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Petrović M, Janakiev T, Grbić ML, Unković N, Stević T, Vukićević S, and Dimkić I
- Subjects
- Plant Development, Bacillus subtilis, Endophytes, Plant Roots microbiology, Phosphates, Beta vulgaris
- Abstract
Sugar beet is the most important crop for sugar production in temperate zones. The plant microbiome is considered an important factor in crop productivity and health. Here, we investigated the bacterial diversity of seeds, roots, and rhizosphere of five sugar beet hybrids named Eduarda (ED), Koala (KO), Tibor (T), Tajfun (TF), and Cercospora-resistant (C). A culture-independent next-generation sequencing approach was used for the further investigation of seed-borne endophytes. Hybrid-associated bacteria were evaluated for their plant growth-promoting (PGP) characteristics, antagonistic activity towards Cercospora beticola and several Fusarium strains in dual culture assays, and drought and salinity tolerance. High-throughput sequencing revealed that the Proteobacteria phylum was most dominant in the seeds of all hybrids, followed by Cyanobacteria and Actinobacteriota. The predominant genus in all hybrids was Pantoea, followed by Pseudomonas, Acinetobacter, Chalicogloea, Corynebacterium, Enterobacter, Enterococcus, Glutamicibacter, Kosakonia, and Marinilactibacillus. Unique genera in the hybrids were Pleurocapsa and Arthrobacter (T), Klebsiella (TF), Apibacter (ED), and Alloscardovia (KO). The genera that were most represented in one hybrid were Weissella and Staphylococcus (TF); Streptococcus (T); Gardnerella, Prevotella, and Rothia (KO); and Gilliamella, Lactobacillus, and Snodgrassella (ED). Thirty-two bacteria out of 156 isolates from the rhizosphere, roots, and seeds were selected with respect to various plant growth-promoting activities in vitro, i.e., nitrogen fixation, phosphate solubilization, siderophore production, indole-3-acetic acid production, 1-aminocyclopropane-1-carboxylic acid deaminase activity, hydrogen cyanide production, exoenzymatic activity (amylase, protease, lipase, cellulase, xylanase, mannanases, gelatinase, and pectinase), mitigation of environmental stresses, and antifungal activity. Mixta theicola KO3-44, Providencia vermicola ED3-10, Curtobacterium pusillum ED2-6, and Bacillus subtilis KO3-18 had the highest potential to promote plant growth due to their multiple abilities (nitrogen fixation, phosphate solubilization, production of siderophores, and IAA). The best antagonistic activity towards phytopathogenic fungi was found for Bacillus velezensis C3-19, Paenibacillus polymyxa C3-36 and Bacillus halotolerans C3-16/2.1. Only four isolates B. velezensis T2-23, B. subtilis T3-4, B. velezensis ED2-2, and Bacillus halotolerans C3-16/2.1 all showed enzymatic activity, with the exception of xylanase production. B. halotolerans C3-16/2.1 exhibited the greatest tolerance to salinity, while two B. subtilis strains (C3-62 and TF2-1) grew successfully at the maximum concentration of PEG. The current study demonstrates that sugar beet-associated bacteria have a wide range of beneficial traits and are therefore highly promising for the formulation of biological control and PGP agents., (© 2023. The Author(s).)
- Published
- 2023
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196. Bacteriobiota and Chemical Changes during the Ripening of Traditional Fermented "Pirot 'Ironed' Sausage".
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Bogdanović S, Stanković S, Berić T, Tomasevic I, Heinz V, Terjung N, and Dimkić I
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"Pirot 'ironed' sausage" (Pis) is a traditional, fermented sausage, made from different types of meat (beef and chevon), without additives or starter cultures. The physical-chemical properties (pH, water activity, fats, moisture, and protein contents) were examined in the initial meat batter stuffing and during ripening. Total bacterial diversity was examined at different time points using both culturable (traditional) and non-culturable (NGS sequencing) approaches. During the ripening, a decrease in pH value, a
w , and moisture content was observed, as well as an increase in protein and fat content. At least a two-fold significant decrease was noted for colorimetric values during the ripening period. The dominance of Proteobacteria and Firmicutes was observed in the non-culturable approach in all studied samples. During the ripening process, an increase in Firmicutes (from 33.5% to 63.5%) with a decrease in Proteobacteria (from 65.4% to 22.3%) was observed. The bacterial genera that were dominant throughout the ripening process were Lactobacillus , Photobacterium , Leuconostoc , Weissella , and Lactococcus , while Carnobacterium , Brochothrix , and Acinetobacter were found also, but in negligible abundance. Among the culturable bacteria, Latilactobacillus sakei ( Lactobacillus sakei ) and Leuconostoc mesenteoides were present in all stages of ripening.- Published
- 2023
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197. Bacteriobiota of the Cave Church of Sts. Peter and Paul in Serbia-Culturable and Non-Culturable Communities' Assessment in the Bioconservation Potential of a Peculiar Fresco Painting.
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Dimkić I, Ćopić M, Petrović M, Stupar M, Savković Ž, Knežević A, Subakov Simić G, Ljaljević Grbić M, and Unković N
- Subjects
- Serbia, Fungi genetics, Antifungal Agents metabolism, Phylogeny, RNA, Ribosomal, 16S genetics, Bacteria metabolism, Proteobacteria genetics
- Abstract
The principal aim of this study was to determine bacterial diversity within the Cave Church of Sts. Peter and Paul, via culturable and non-culturable approaches, and elucidate the antifungal potential of autochthonous antagonistic bacterial isolates against biodeteriogenic fungi. Furthermore, whole-genome sequencing of selected bacterial antagonists and the analysis of genes included in the synthesis of secondary metabolites were performed. With the highest RA values, determined in metabarcoding analysis, phyla Actinobacteriota (12.08-54.00%) and Proteobacteria (25.34-44.97%) dominated most of the samples. A total of 44 different species, out of 96 obtained isolates, were determined as part of the culturable bacteriobiota, with the predominance of species from the genus Bacillus . Bacillus simplex was the only isolated species simultaneously present in all investigated substrata within the church. The best antagonistic activity against 10 biodeteriogenic fungi was documented for Streptomyces anulatus , followed by Bacillus altitudinis , Chryseobacterium viscerum , and Streptomyces sp. with their highest PGI% values ranging of from 55.9% to 80.9%. These promising results indicate that characterized bacteria are excellent candidates for developing biocontrol strategies for suppressing deteriogenic fungi responsible for the deterioration of investigated fresco painting. Finally, isolate 11-11MM, characterized as Streptomyces sp., represents a new species for science prompting the need for further study.
- Published
- 2023
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198. Mycobiome Diversity of the Cave Church of Sts. Peter and Paul in Serbia-Risk Assessment Implication for the Conservation of Rare Cavern Habitat Housing a Peculiar Fresco Painting.
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Ljaljević Grbić M, Dimkić I, Savković Ž, Stupar M, Knežević A, Jelikić A, and Unković N
- Abstract
The mycobiome of the cave Church of Sts. Peter and Paul, housing the peculiar fresco painting of “The Bald-headed Jesus”, was analyzed via culture-dependent and -independent methods. Salt efflorescence, colored patinas, and biofilm, as well as biopitting, discolorations, and fruiting bodies of wood-decay fungi were observed on surfaces within the church. Microscopic analyses showed an abundance of fungal structures, i.e., conidiophores, conidia, chlamydospores, and ascospores. The estimated values of the contamination classified all surfaces as the “Danger zone”. A total of 24 fungi from 17 genera were determined as part of the culturable mycobiome, with a dominance of Ascomycota of genera Penicillium. Biodegradative profiles analyzed via plate assays demonstrated positive reactions for 16 isolates: most commonly acid production (8), followed by pigment production and ligninolytic activity (6), protein degradation (5), cellulolytic activity (3) and carbonate dissolution (2). Metabarcoding analysis showed a dominance of Ascomycota in all samples (79.9−99.7%), with high relative abundance documented for Hypoxylon fuscopurpureum on the iconostasis and unclassified Mycosphaerellaceae family within order Capnodiales on fresco and stone, as well as moderate relative abundance for unclassified Dothideomycetes, Botryolepraria lesdainii, Verrucaria sp. and Cladosporium sp. on stone walls. The used set of integrative methods pointed out species of genus Neodevriesia and H. fuscopurpureum as the main deteriogenic agents of fresco and iconostasis surfaces, respectively.
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- 2022
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199. The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates.
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Janakiev T, Berić T, Stević T, Stanković S, Bačić J, Majstorović H, Fira D, and Dimkić I
- Abstract
The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed Pantoea , Sphingomonas , Hymenobacter , Massilia , and Pseudomonas as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were Metschnikowia , Filobasidium , Aureobasidium pullulans , Botrytis cinerea , and Taphrina . The traditional culturable approach revealed that the Pseudomonas genus with P. graminis , P. putida , and P. congelans was most prevalent. The most frequently cultivated fungal representatives belonged to the genus Fusarium with six identified species. A broad range of the antagonistic activity was detected for the Hannaella luteola and Metschnikowia pulcherrima yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. Fusarium sporotrichioides was the most susceptible fungal isolate. The autochthonous antagonistic yeasts H. luteola and M. pulcherrima might be powerful biological control agents of postharvest diseases caused by Fusarium spp. and common pathogens like Monilinia laxa , Botrytis cinerea , Alternaria tenuissima , and Cladosporium cladosporioides .
- Published
- 2022
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200. Xanthomonas bonasiae sp. nov. and Xanthomonas youngii sp. nov., isolated from crown gall tissues.
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Mafakheri H, Taghavi SM, Zarei S, Portier P, Dimkić I, Koebnik R, Kuzmanović N, and Osdaghi E
- Subjects
- Bacterial Typing Techniques, Base Composition, Crops, Agricultural genetics, DNA, Bacterial genetics, Fatty Acids chemistry, Nucleic Acid Hybridization, Phylogeny, Plant Tumors microbiology, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Xanthomonas
- Abstract
The genus Xanthomonas contains a set of diverse bacterial strains, most of which are known for their pathogenicity on annual crops and fruit trees causing economically important plant diseases. Recently, five Xanthomonas strains were isolated from Agrobacterium -induced crown gall tissues of amaranth ( Amaranthus sp.) and weeping fig ( Ficus benjamina ) plants in Iran. Phenotypic characteristics ( i.e . biochemical tests and pathogenicity features) and whole genome sequence-based core-genome phylogeny followed by average nucleotide identity and digital DNA-DNA hybridization calculations suggested that these gall-associated strains belong to two new species within the genus Xanthomonas . In this study, we provide a formal species description for these new species where Xanthomonas bonasiae sp. nov. is proposed for the strains isolated from weeping fig with FX4
T (=CFBP 8703T =DSM 112530T ) as type strain. The name Xanthomonas youngii sp. nov. is proposed for the strains isolated from amaranth with AmX2T (=CFBP 8902T =DSM 112529T ) as type strain.- Published
- 2022
- Full Text
- View/download PDF
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