7,590 results on '"Decio, A"'
Search Results
152. PDIA3 orchestrates effector T cell program by serving as a chaperone to facilitate the non-canonical nuclear import of STAT1 and PKM2
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Yang, Chunliang, Wang, Fa Xi, Luo, Jiahui, Rong, Shan Jie, Lu, Wan Ying, Chen, Qi Jie, Xiao, Jun, Wang, Ting, Song, Dan Ni, Liu, Jing, Mo, Qian, Li, Shuo, Chen, Yu, Wang, Ya Nan, Liu, Yan Jun, Yan, Tong, Gu, Wei Kuan, Zhang, Shu, Xiong, Fei, Yu, Qilin, Zhang, Zi Yun, Yang, Ping, Liu, Shi-Wei, Eizirik, Decio L., Dong, Ling Li, Sun, Fei, Wang, Cong Yi, Yang, Chunliang, Wang, Fa Xi, Luo, Jiahui, Rong, Shan Jie, Lu, Wan Ying, Chen, Qi Jie, Xiao, Jun, Wang, Ting, Song, Dan Ni, Liu, Jing, Mo, Qian, Li, Shuo, Chen, Yu, Wang, Ya Nan, Liu, Yan Jun, Yan, Tong, Gu, Wei Kuan, Zhang, Shu, Xiong, Fei, Yu, Qilin, Zhang, Zi Yun, Yang, Ping, Liu, Shi-Wei, Eizirik, Decio L., Dong, Ling Li, Sun, Fei, and Wang, Cong Yi
- Abstract
Dysregulated T cell activation underpins the immunopathology of rheumatoid arthritis (RA), yet the machineries that orchestrate T cell effector program remain incompletely understood. Herein, we leveraged bulk and single-cell RNA sequencing data from RA patients and validated protein disulfide isomerase family A member 3 (PDIA3) as a potential therapeutic target. PDIA3 is remarkably upregulated in pathogenic CD4 T cells derived from RA patients and positively correlates with C-reactive protein level and disease activity score 28. Pharmacological inhibition or genetic ablation of PDIA3 alleviates RA-associated articular pathology and autoimmune responses. Mechanistically, T cell receptor signaling triggers intracellular calcium flux to activate NFAT1, a process that is further potentiated by Wnt5a under RA settings. Activated NFAT1 then directly binds to the Pdia3 promoter to enhance the expression of PDIA3, which complexes with STAT1 or PKM2 to facilitate their nuclear import for transcribing T helper 1 (Th1) and Th17 lineage-related genes, respectively. This non-canonical regulatory mechanism likely occurs under pathological conditions, as PDIA3 could only be highly induced following aberrant external stimuli. Together, our data support that targeting PDIA3 is a vital strategy to mitigate autoimmune diseases, such as RA, in clinical settings., SCOPUS: ar.j, info:eu-repo/semantics/published
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- 2024
153. Presenting Nonreflexive Quantum Mechanics: Formalism and Metaphysics
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Krause, Decio and Arenhart, Jonas R. B.
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Quantum Physics ,Physics - History and Philosophy of Physics ,81P70, 03E10, 81P05 - Abstract
Nonreflexive quantum mechanics is a formulation of quantum theory based on a non-classical logic termed \ita{nonreflexive logic} (a.k.a. `non-reflexive'). In these logics, the standard notion of identity, as encapsulated in classical logic and set theories, does not hold in full. The basic aim of this kind of approach to quantum mechanics is to take seriously the claim made by some authors according to whom quantum particles are \ita{non-individuals} in some sense, and also to take into account the fact that they may be absolutely indistinguishable (or indiscernible). The nonreflexive formulation of quantum theory assumes these features of the objects already at the level of the underlying logic, so that no use is required of symmetrization postulates or other mathematical devices that serve to pretend that the objects are indiscernible (when they are not: all objects that obey classical logic are \ita{individuals} in a sense). Here, we present the ideas of the development of nonreflexive quantum mechanics and discuss some philosophical (mainly metaphysical) motivations and consequences of it., Comment: 22 pages, no figures, accepted paper
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- 2015
154. A logical account of quantum superpositions
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Krause, Decio and Arenhart, Jonas R. B.
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Quantum Physics ,Mathematics - Logic ,81P10, 81P05, 03B45 - Abstract
In this paper we consider the phenomenon of superpositions in quantum mechanics and suggest a way to deal with the idea in a logical setting from a syntactical point of view, that is, as subsumed in the language of the formalism, and not semantically. We restrict the discussion to the propositional level only. Then, after presenting the motivations and a possible world semantics, the formalism is outlined and we also consider within this schema the claim that superpositions may involve contradictions, as in the case of the Schr\"odinger's cat, which (it is usually said) is both alive and dead. We argue that this claim is a misreading of the quantum case. Finally, we sketch a new form of quantum logic that involves three kinds of negations and present the relationships among them. The paper is a first approach to the subject, introducing some main guidelines to be developed by a `syntactical' logical approach to quantum superpositions., Comment: 17 pages, forthcoming in Probing the Meaning and Structure of Quantum Mechanics: Superpositions, Semantics, Dynamics and Identity, D. Aerts, C. de Ronde, H. Freytes and R. Giuntini (Eds.), World Scientific, Singapore, 2015
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- 2015
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155. The non autonomous YdKN equation and generalized symmetries of Boll equations
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Gubbiotti, Giorgio, Scimiterna, Christian, and Levi, Decio
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Nonlinear Sciences - Exactly Solvable and Integrable Systems ,Mathematical Physics ,39-XX, 70G65, 37K10 - Abstract
In this paper we study the integrability of a class of nonlinear non autonomous quad graph equations compatible around the cube introduced by Boll. We show that all these equations possess three point generalized symmetries which are subcases of either the Yamilov discretization of the Krichever--Novikov equation or of its non autonomous extension. We also prove that all those symmetries are integrable as pass the algebraic entropy test.
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- 2015
156. Formation of the Janus-Epimetheus system through collisions
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Treffenstädt, Lucas Luigi, Mourão, Décio C., and Winter, Othon C.
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Astrophysics - Earth and Planetary Astrophysics - Abstract
Context: Co-orbital systems are bodies that share the same mean orbit. They can be divided into different families according to the relative mass of the co-orbital partners and the particularities of their movement. Janus and Epimetheus are unique in that they are the only known co-orbital pair of comparable masses and thus the only known system in mutual horseshoe orbit. Aims: We aim to establish whether the Janus-Epimetheus system might have formed by disruption of an object in the current orbit of Epimetheus. Methods: We assumed that four large main fragments were formed and neglected smaller fragments. We used numerical integration of the full N-body problem to study the evolution of different fragment arrangements. Collisions were assumed to result in perfectly inelastic merging of bodies. We statistically analysed the outcome of these simulations to infer whether co-orbital systems might have formed from the chosen initial conditions. Results: Depending on the range of initial conditions, up to 9% of the simulations evolve into co-orbital systems. Initial velocities around the escape velocity of Janus yield the highest formation probability. Analysis of the evolution shows that all co-orbital systems are produced via secondary collisions. The velocity of these collisions needs to be low enough that the fragments can merge and not be destroyed. Generally, collisions are found to be faster than an approximate cut-off velocity threshold. However, given a sufficiently low initial velocity, up to 15% of collisions is expected to result in merging. Hence, the results of this study show that the considered formation scenario is viable., Comment: 9 Pages, 8 Figures
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- 2015
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157. Prime Focus Spectrograph for the Subaru telescope: massively multiplexed optical and near-infrared fiber spectrograph
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Sugai, Hajime, Tamura, Naoyuki, Karoji, Hiroshi, Shimono, Atsushi, Takato, Naruhisa, Kimura, Masahiko, Ohyama, Youichi, Ueda, Akitoshi, Aghazarian, Hrand, de Arruda, Marcio Vital, Barkhouser, Robert H., Bennett, Charles L., Bickerton, Steve, Bozier, Alexandre, Braun, David F., Bui, Khanh, Capocasale, Christopher M., Carr, Michael A., Castilho, Bruno, Chang, Yin-Chang, Chen, Hsin-Yo, Chou, Richard C. Y., Dawson, Olivia R., Dekany, Richard G., Ek, Eric M., Ellis, Richard S., English, Robin J., Ferrand, Didier, Ferreira, Décio, Fisher, Charles D., Golebiowski, Mirek, Gunn, James E., Hart, Murdock, Heckman, Timothy M., Ho, Paul T. P., Hope, Stephen, Hovland, Larry E., Hsu, Shu-Fu, Hu, Yen-Shan, Huang, Pin Jie, Jaquet, Marc, Karr, Jennifer E., Kempenaar, Jason G., King, Matthew E., Fèvre, Olivier Le, Mignant, David Le, Ling, Hung-Hsu, Loomis, Craig, Lupton, Robert H., Madec, Fabrice, Mao, Peter, Marrara, Lucas Souza, Ménard, Brice, Morantz, Chaz, Murayama, Hitoshi, Murray, Graham J., de Oliveira, Antonio Cesar, de Oliveira, Claudia Mendes, de Oliveira, Ligia Souza, Orndorff, Joe D., Vilaça, Rodrigo de Paiva, Partos, Eamon J., Pascal, Sandrine, Pegot-Ogier, Thomas, Reiley, Daniel J., Riddle, Reed, Santos, Leandro, Santos, Jesulino Bispo dos, Schwochert, Mark A., Seiffert, Michael D., Smee, Stephen A., Smith, Roger M., Steinkraus, Ronald E., Sodré Jr, Laerte, Spergel, David N., Surace, Christian, Tresse, Laurence, Vidal, Clément, Vives, Sebastien, Wang, Shiang-Yu, Wen, Chih-Yi, Wu, Amy C., Wyse, Rosie, and Yan, Chi-Hung
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Astrophysics - Instrumentation and Methods for Astrophysics - Abstract
The Prime Focus Spectrograph (PFS) is an optical/near-infrared multifiber spectrograph with 2394 science fibers distributed across a 1.3-deg diameter field of view at the Subaru 8.2-m telescope. The wide wavelength coverage from 0.38 {\mu}m to 1.26 {\mu}m, with a resolving power of 3000, simultaneously strengthens its ability to target three main survey programs: cosmology, galactic archaeology and galaxy/AGN evolution. A medium resolution mode with a resolving power of 5000 for 0.71 {\mu}m to 0.89 {\mu}m will also be available by simply exchanging dispersers. We highlight some of the technological aspects of the design. To transform the telescope focal ratio, a broad-band coated microlens is glued to each fiber tip. A higher transmission fiber is selected for the longest part of the cable system, optimizing overall throughput; a fiber with low focal ratio degradation is selected for the fiber-positioner and fiber-slit components, minimizing the effects of fiber movements and fiber bending. Fiber positioning will be performed by a positioner consisting of two stages of piezo-electric rotary motors. The positions of these motors are measured by taking an image of artificially back-illuminated fibers with the metrology camera located in the Cassegrain container; the fibers are placed in the proper location by iteratively measuring and then adjusting the positions of the motors. Target light reaches one of the four identical fast-Schmidt spectrograph modules, each with three arms. The PFS project has passed several project-wide design reviews and is now in the construction phase., Comment: 19 pages, 11 figures
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- 2015
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158. Structure Preserving Discretizations of the Liouville Equation and their Numerical Tests
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Levi, Decio, Martina, Luigi, and Winternitz, Pavel
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Nonlinear Sciences - Exactly Solvable and Integrable Systems ,Mathematical Physics - Abstract
The main purpose of this article is to show how symmetry structures in partial differential equations can be preserved in a discrete world and reflected in difference schemes. Three different structure preserving discretizations of the Liouville equation are presented and then used to solve specific boundary value problems. The results are compared with exact solutions satisfying the same boundary conditions. All three discretizations are on four point lattices. One preserves linearizability of the equation, another the infinite-dimensional symmetry group as higher symmetries, the third one preserves the maximal finite-dimensional subgroup of the symmetry group as point symmetries. A 9-point invariant scheme that gives a better approximation of the equation, but significantly worse numerical results for solutions is presented and discussed.
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- 2015
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159. I Congresso Brasileiro Sobre Doenças Raras - I CONBRASDRA
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Lima, Lucas Santos, primary, Miranzi, Mário Alfredo Silveira, additional, Vieira, Larissa Fernanda Medeiros, additional, Gonçalves, Isabela de Souza, additional, Lima, Matheus Santos, additional, Reis Filho, Lupércio Rocha, additional, Andrade, Gustavo Medeiros, additional, Silva Filho, Antônio José Magalhães, additional, Estácio, Rayssa Carolinne Costa Mota, additional, Silva, Décio Fragata da, additional, Silveira, Danilo José de Andrade Santos, additional, Andrade, Thiago Vaz de, additional, Lima, Yvnna Santos, additional, Duarte, Meyling Eng de Almeida, additional, Chagas, Arthur Matos Bitencourt, additional, Vasconcelos, Fernanda de Castro de, additional, Araujo, Ângelo Marcel Santana Duarte, additional, Santana, José Vinícius Lima, additional, Sobral Neto, José Monteiro, additional, Barboza, Jully Cristina Vilar, additional, Mellara, Gabriel Guimarães, additional, Vieira, Arthur Sobral, additional, VALADARES, FABIANA TORRES, additional, Bomfim Neto, Raul, additional, Galindo, Renata Feitosa, additional, Diniz, Sophia Rezende, additional, MONTE, KAHENA MONTEIRO ALMEIDA, additional, Barros, Pablo da Cruz, additional, Goes, Vinicius, additional, Gomes, Melissa Vieira, additional, Gomes, José Natthanael de Aquino, additional, Pachêco, Bruna de Lima, additional, Melo, Josevaldo Araújo de, additional, Ferreira, Emanuela Altmann, additional, Moraes, Thiago Henrique da Silva, additional, Sá, Maria Hélida Alves Callou de, additional, Silvestre, Zenilda Gueiros, additional, Silva, Millena Nayara da, additional, Campagnolo, Ângela Quatrin, additional, Barreto, D'Angelis dos Anjos, additional, Costa, Maria Vitória Pinheiro da, additional, Ferreira, Sara Alves, additional, Melo, Janaína de Oliveira, additional, Castro, Amanda Miranda, additional, Lima, Kelen Cristina Sant'Anna de, additional, Nunes, Ana Paula Nogueira, additional, Alves, Paula Aryane Brito, additional, Starling, Ana Lúcia Pimenta, additional, Miranda, Arícia Gomes, additional, Faustino, Luciana Rocha, additional, Cunha, Sofia Carneiro da, additional, Pereira, Breno Serafim, additional, Silva, Kleyciane Kévilin Pereira da, additional, Melo, Ricardo Eugenio Varela Ayres de, additional, Paiva, Patrícia Sthefânia Mulatinho, additional, Silva, Alana Milena Honorato, additional, Queiroz, José Thomas Azevedo de, additional, Salvador, Viviane de Albuquerque Azevedo, additional, Santos, Luana Maria de Moura, additional, Andrade, Ana Carolina Soares de, additional, Rocha, Bruna Thaís Santos da, additional, Fernandes, Marcela Côrte Real, additional, Melo, Ricardo Eugênio Varela Ayres de, additional, Gontijo, Maria Clara de Araújo, additional, Gama, Henrique Valladão Pires, additional, CAMPOS, EDUARDA MEDEIROS, additional, Mariante, Luiza Thomopoulos, additional, Avelar, Marcos Salomão Staut, additional, Lemos, Maria Eduarda Ferreira, additional, Feitosa, Polyana Santos, additional, Viana, Thiago José Magalhães Silva, additional, Dantas, Cley Gabriel Lima Carvalho, additional, Dantas, Vanessa Silva, additional, Santana, Kathllen Corrêa, additional, Andrade, José Vinícius Rezende de, additional, Dinísio, Társilla de Menezes, additional, Jesus, Aryel Andrade de, additional, Santana, Maria Eduarda Oliveira, additional, Lima, Liliane Santos, additional, Souza, Danilo Santos, additional, Nascimento Filho, Vital Menezes do, additional, Perígolo, Lavínia Barbosa da Terra, additional, Souza, Ana Cláudia de, additional, Assunção, Cláudia Gomes, additional, Pacciulli, Letícia Pinheiro Maia, additional, Barreto, Yesly Marinho da Rocha, additional, Silva, Hozana Fernanda da, additional, Arruda, Maria Dhescyca Ingrid Silva, additional, Bezerra, Maria Eduarda Lopes de Macedo, additional, Gabriel, Isabella Gonçalves, additional, Andrade, Carlos Eugênico Fernandez de, additional, Mancini, Camila dos Santos, additional, Abbamonti, Giulia Afonso, additional, Ferreyra, Heloisa Correia de Morais, additional, Neves, Nathalia Meneses, additional, Jordao, Nicole Oliveira, additional, Mauro, Athene Maria de Marco França, additional, Godoi, Lorena Fontinele, additional, Nunes, Jomar Diogo Costa, additional, Silva, Vitória Regina Vidal Sabá e, additional, Nascimento, Antonia Amanda Cortez do, additional, Cortêz, Thayná Huet Carneiro, additional, Leite Neto, Felipe, additional, Nascimento, Antônia Amanda Cortez do, additional, Chemello, Raissa Massaia Londero, additional, Silva, Millena Nayara Da, additional, Barreto, Dangelis dos Anjos, additional, Farias, Raquel Viégas Martins, additional, Santos, João Paulo Velloso Medrado, additional, Almeida, Camille Teles Ferreira de, additional, Ramos, Dandara Rocha, additional, Aguiar, Manuela Santana, additional, Almeida, Elielton de Jesus, additional, Alves, Yorrane Kelly Gomes, additional, Santos, Igor Luiz Vieira de Lima, additional, Silva, Maria Eduarda Wanderley de Barros, additional, Araújo, Tainá Oliveira de, additional, Cruz, Ellen Karolaine Lucena da, additional, Souza, Juliana Araujo de, additional, França, Beatriz Paulina Santos, additional, Oliveira, Maria Luiza Farias, additional, Santos, Quézia Ellen da Silva, additional, Rodrigues, Anne Wirginne de Lima, additional, Ferreira, Jayana Gabrielle Sobral, additional, Silva, Nephtys Verissimo da, additional, Barros, Mateus Silva de, additional, Araújo, Amanda Geovana Pereira de, additional, Feitosa, Paula Yhasmym de Oliveira, additional, Melo, Josevaldo Araujo de, additional, Silvestre, Zenila Gueiros, additional, Rodrigues, Joana Rosa, additional, Barbisan, Fernanda, additional, Ferreira, Wellington Claudino, additional, PINTO, GUSTAVO DOMINGOS MELO, additional, Santos, Jordan Ramos, additional, Dias, Simone Braga, additional, Silva, Wanusa Belarmino da, additional, Rodrigues, Geicy Bianca Martins, additional, Batalha, Murilo Fialho, additional, Ferrante, Igor Mello, additional, Santos, Mariana da Silva, additional, Wojciechovski, Júlia, additional, Pereira, Mariana Schenato Araujo, additional, Traub, Anna Clara, additional, Mendes, Carolina Dusi, additional, Correa, Giovana Luiza, additional, Pereira, Mariana Araujo Schenato, additional, Silva, Decio Fragata Da, additional, Estacio, Rayssa Carolinne Costa Mota, additional, Araujo, Angelo Marcel Santana Duarte, additional, Bonfim Neto, Raul, additional, Limav, Yvnna Santos, additional, Barboza, Jully Cristina Villar, additional, Vasconcelos, Fernanda de Castro, additional, Santos, Áleff Edrei Gomes, additional, Ferraro, Halley Oliveira, additional, Silveira, Danilo Jose de Andrade Santos, additional, COUTO, LETÍCIA MACHADO, additional, Silva, Yuri Cássio de Lima, additional, Aguiar, Camila Siqueira, additional, Melo, Rodrigo Henrique Melo Varela Ayres de, additional, Rhoden, Deise Louise Bohn, additional, Melo, Milena Melo Varela Ayres de, additional, Melo, Victor Leonardo Melo Varela Ayres de, additional, Melo Júnior, Frederico Márcio Varela Ayres de, additional, Melo, Bruna Heloísa Costa Varela Ayres de, additional, Beck, Júlia Souza, additional, Lira, Luiz Henrique Campello, additional, Seixas, Zélia de Albuquerque, additional, Freitas, Nela Dulce Varela de Melo Costa, additional, Melo, Jussara Diana Varela Ayres de, additional, Sousa, Douglas Monteiro de, additional, Silva, Luísa Margareth Carneiro da, additional, Cabral, Ananda Leticia Silva, additional, Gonçalves, Elielson Felix, additional, Arruda, Isabela Tatiana Sales de, additional, Paiva, Wemerson Sousa, additional, Xavier, Barbara Julia Rodrigues, additional, Alvarenga, Pedro Victor Santana, additional, Matias, Tamires de Alexandria, additional, oliveira, Halley ferraro, additional, Chagas, Arthur Matos Bittencourt, additional, Silveira, Danilo José Andrade dos Santos, additional, Santana, José Vinicius Lima, additional, Duarte, Ângelo Marcel Santana, additional, Duarte, Meylling Eng de Almeida, additional, Ferreira Filho, Ecliê Santos, additional, Campos, Ana Helena Prado Santana, additional, Santana, Letícia Brandão, additional, Souza, Anelise Marques Feitosa de, additional, Cordeiro, Maria Eduarda Santana, additional, Rosa, Heloísa, additional, Marinheiro, Juliana Cristina, additional, Martins Neto, José da Silva, additional, Carmo, Monique Santos do, additional, Neves, Julia Alim Maluf, additional, Sousa Junior, Paulo Ferreira de, additional, Alves, Yorrane kelly Gomes, additional, Oliveira, Halley Ferraro, additional, Alves, Matheus Porto, additional, Carvalho, Letícia Gonçalves, additional, Paoliello, Adriana Balbina, additional, Fogari, Letícia Raiane, additional, Caparroz, Maria Eduarda Leati, additional, Sousa, Daniela Heitzmann Amaral Valentim de, additional, Alves, Yorrane kelly gomes, additional, Bomfim, Vitória Vilas Boas da Silva, additional, Diniz, Samara Dantas de Medeiros, additional, Galvão, Natallie Cecília dos Santos, additional, Vieira, Walisson da Silva, additional, Fernandes, Nathalia Emmanuele Pereira, additional, Oliveira, Bianca Diniz, additional, RIBEIRO, Ricardo de Souza, additional, Duarte, Henrique Maciel, additional, Araújo Junior, José Geraldo, additional, BRAGA, Maria Gabriella Borges, additional, Oliveira, Vinícius Carvalho de, additional, Ribeiro, Ricardo de Souza, additional, Dias, Sylvia Carriel, additional, Freire, Márcia Helena de Souza, additional, Saganski, Gabrielle Freitas, additional, Lima, Lohana Maylane Aquino Correia de, additional, Aguiar, Camilla Siqueira de, additional, Melo, Victor Leonardo Mello Varela Ayres de, additional, Melo Junior, Frederico Marcio Varela Ayres de, additional, Beck, Júlia de Souza, additional, Melo, Rodrigo Henrique Mello Varela Ayres de, additional, Pinheiro, Milena Mello Varela Ayres de Melo, additional, Freitas, Nely Dulce Varela de Melo Costa, additional, Nascimento, Antonia Amanda Cortêz do, additional, Araújo, Iara Medeiros de, additional, Alavrenga, Pedro Victor Santana, additional, Assis, Bruno Mouallem de, additional, Pinto, Gustavo Domingos Melo, additional, Temponi, Bárbara Vilaça, additional, Chaves, Ana Luisa Rogana, additional, Faria, Arthur Pereira Barbosa de, additional, Costa, Carolina Siqueira Florentino, additional, Pimenta, Pedro Pires Costa, additional, Paiva, Patricia Sthefânia Mulatinho, additional, Pereira, Ayane Paula Mendonça, additional, Dellalibera, Edileine, additional, Sousa, Laila Eliza Teixeira de, additional, Paula, Ana Karolina Nascimento, additional, Alves, Even Herlany Pereira, additional, Oliveira, Pedro Henrique Sales de, additional, Pontes, Vitória de Melo, additional, Silva, Diego dos Santos, additional, Leite, Igor José Paiva, additional, Martins, Willian Silva, additional, Gomes, Alanna Carvalho, additional, chagas, arthur matos bitencourt, additional, ferreira, isabella lisboa, additional, lima, yvnna santos, additional, ferreira filho, eclie santos, additional, campos, ana helena prado, additional, silveira, danilo jose de andrade santos, additional, Tenório Neto, José Alves, additional, Albuquerque, Amanda Oliveira Bernardino Cavalcanti de, additional, marques, Rebeca Ribeiro, additional, Santos, Dayane Priscila dos, additional, Hessmann, Giovanna, additional, and Pinto, Luanna, additional
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- 2021
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160. Water budget fluxes in catchments under grassland and Eucalyptus plantations of different ages
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Ferreto, Decio Oscar Cardoso, Reichert, Jose Miguel, Cavalcante, Rosane Barbosa Lopes, and Srinivasan, Raghavan
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Brazil -- Environmental aspects -- Natural resources ,Evapotranspiration -- Observations ,Tree farms -- Environmental aspects ,Water balance (Hydrology) -- Forecasts and trends ,Grasslands -- Physiological aspects -- Growth ,Watersheds -- Protection and preservation ,Eucalyptus -- Growth -- Physiological aspects ,Company growth ,Market trend/market analysis ,Earth sciences - Abstract
Planted forests are increasing in area worldwide in recent decades and play an important role in carbon sequestration programs. However, the effects of plantations on water resources are largely unknown. Here, we investigate the effects of Eucalyptus sp. plantations on water budget fluxes in the southern Brazilian grasslands biome. We evaluated green (canopy interception and evapotranspiration) and blue (discharge) water flows in three watersheds: two watersheds predominantly covered with eucalyptus, either in the first years after planting or at the end of the rotation, and one watershed with livestock-grazing grassland. We used field measurements of rainfall, streamflow, and throughfall and estimated canopy interception and evapotranspiration by water balance. Water flows in the monitored watersheds with eucalyptus plantations were influenced by forest developmental stage. Annual canopy interception and transpiration were always higher in the watersheds with eucalyptus than in the one with grassland, except for the transpiration in the first year after plantation in the watershed with young eucalyptus. An increase in evapotranspiration (green water flow) and the consequent decrease in streamflow (blue water flow) should be considered in local water resource management. Studies of catchment hydrology and forest management for improved water use efficiency and streamflow regulation are needed, particularly in understudied regions. Key words: forest hydrology, evapotranspiration, streamflow, canopy interception, Eucalyptus production, southern grasslands. La superficie des plantations forestieres a augmente au cours des dernieres decennies et celles-ci jouent un role important dans les programmes de sequestration du carbone. Cependant, les effets des plantations d'Eucalyptus sp. sur les ressources hydriques dans le biome de la prairie meridionale du Bresil sont largement inconnus et, dans cet article, nous abordons les flux du bilan hydrique incluant les flux verts et bleus. Nous avons evalue les flux hydriques verts (interception et evapotranspiration du couvert forestier) et bleus (debit des cours d'eau) dans trois bassins versants: deux bassins avec un couvert principalement compose d'eucalyptus, soit durant les deux premieres annees apres la plantation ou a la fin de la rotation, et un autre bassin occupe par une prairie laissee en paturage pour le betail. Nous avons utilise des mesures prises sur le terrain de la precipitation, de l'ecoulement fluvial et de la pluie au sol, et nous avons estime l'interception et l'evapotranspiration du couvert forestier au moyen du bilan hydrique. Le debit d'eau dans les bassins versants sous etude occupes par des plantations d'eucalyptus etait influence par le stade de developpement de la foret. L'interception et la transpiration annuelles du couvert forestier etaient toujours plus elevees dans les bassins versants ou l'eucalyptus etait present que dans les bassins versants occupes par la prairie, a l'exception de la transpiration durant la premiere annee apres la plantation dans le bassin versant occupe par de jeunes eucalyptus. On devrait tenir compte de l'augmentation de l'evapotranspiration (flux hydrique vert) et de la diminution consecutive de l'ecoulement fluvial (flux hydrique bleu) dans la gestion locale des ressources hydriques. Les etudes sur les bassins hydrographiques et l'amenagement forestier pour ameliorer l'efficacite de l'utilisation de l'eau et la regularisation des cours d'eau sont necessaires, particulierement dans les regions peu etudiees. [Traduit par la Redaction] Mots-cles : hydrologie forestiere, evapotranspiration, ecoulement fluvial, interception du couvert forestier, production d'Eucalyptus, prairies meridionales., 1. Introduction The growing global demand for forest products is contributing to the expansion of fast-growing tree plantations, where Eucalyptus sp. is often the main genus used to meet this [...]
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- 2021
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161. DEMARKETING E OPORTUNIDADES ESTRATÃGICAS PARA O AGRONEGÃ'CIO EM MOGI DAS CRUZES/SP: MARCAS ALINHADAS AOS OBJETIVOS GLOBAIS--PRÃTICAS DE PESQUISA TECNOLÃ'GICAS/DEMARKETING AND STRATEGIC OPPORTUNITIES FOR AGRIBUSINESS IN MOGI DAS CRUZES/SP: BRANDS ALIGNED TO GLOBAL OBJECTIVES - TECHNOLOGICAL RESEARCH PRACTICES/DEMARKETING Y OPORTUNIDADES ESTRATÃGICAS PARA LA AGROINDUSTRIA EN MOGI DAS CRUZES/SP: MARCAS ALINEADAS CON OBJETIVOS GLOBALES - PRÃCTICAS DE INVESTIGACIÃ'N TECNOLÃ'GICA
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Forni, Decio Ferreira and Romanini, Anderson VinÃcius
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- 2023
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162. Effect of Carbon Nanotubes on the Na+ Intercalation Capacity of Binder Free Mn2V2O7-CNTs Electrode: A Structural Investigation
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Rahul Parmar, Javad Rezvani, Matteo Amati, Luca Gregoratti, Decio Batista de Freitas Neto, Jose Mauricio Rosolen, and Roberto Gunnella
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MVO-CNTs composite ,CNTs mass loading ,X-ray imaging ,cathode electrolyte interphase (CEI) ,binder-free NIBs electrode ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Improvements in sodium intercalation in sodium cathodes have been debated in recent years. In the present work, we delineate the significant effect of the carbon nanotubes (CNTs) and their weight percent in the intercalation capacity of the binder-free manganese vanadium oxide (MVO)-CNTs composite electrodes. The performance modification of the electrode is discussed taking into account the cathode electrolyte interphase (CEI) layer under optimal performance. We observe an intermittent distribution of the chemical phases on the CEI, formed on these electrodes after several cycles. The bulk and superficial structure of pristine and Na+ cycled electrodes were identified via micro-Raman scattering and Scanning X-ray Photoelectron Microscopy. We show that the inhomogeneous CEI layer distribution strongly depends on the CNTs weight percentage ratio in an electrode nano-composite. The capacity fading of MVO-CNTs appears to be associated with the dissolution of the Mn2O3 phase, leading to electrode deterioration. This effect is particularly observed in electrodes with low weight percentage of the CNTs in which the tubular topology of the CNTs are distorted due to the MVO decoration. These results can deepen the understanding of the CNTs role on the intercalation mechanism and capacity of the electrode, where there are variations in the mass ratio of CNTs and the active material.
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- 2023
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163. The ER stress response mediator ERO1 triggers cancer metastasis by favoring the angiogenic switch in hypoxic conditions
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Varone, Ersilia, Decio, Alessandra, Chernorudskiy, Alexander, Minoli, Lucia, Brunelli, Laura, Ioli, Federica, Piotti, Arianna, Pastorelli, Roberta, Fratelli, Maddalena, Gobbi, Marco, Giavazzi, Raffaella, and Zito, Ester
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- 2021
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164. Towards a new model and classification of mood disorders based on risk resilience, neuro-affective toxicity, staging, and phenome features using the nomothetic network psychiatry approach
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Maes, Michael, Moraes, Juliana Brum, Bonifacio, Kamila Landucci, Barbosa, Decio Sabbatini, Vargas, Heber Odebrecht, Michelin, Ana Paula, and Nunes, Sandra Odebrecht Vargas
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- 2021
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165. Pseudohyperaldosteronism Due to Licorice: A Practice-Based Learning from a Case Series.
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Sabbadin, Chiara, Graziani, Andrea, Bavaresco, Alessandro, Mazzeo, Pierluigi, Tizianel, Irene, Ceccato, Filippo, Armanini, Decio, and Barbot, Mattia
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MINERALOCORTICOID receptors ,POTASSIUM antagonists ,HYPOKALEMIA ,HYPERTENSION ,RENIN ,ALDOSTERONE antagonists ,LAXATIVES - Abstract
Pseudohyperaldosteronism (PHA) is characterized by hypertension, hypokalemia, and a decrease in plasma renin and aldosterone levels. It can be caused by several causes, but the most frequent is due to excess intake of licorice. The effect is mediated by the active metabolite of licorice, glycyrrhetinic acid (GA), which acts by blocking the 11-hydroxysteroid dehydrogenase type 2 and binding to the mineralocorticoid receptor (MR) as an agonist. The management of licorice-induced PHA depends on several individual factors, such as age, gender, comorbidities, duration and amount of licorice intake, and metabolism. The clinical picture usually reverts upon licorice withdrawal, but sometimes mineralocorticoid-like effects can be critical and persist for several weeks, requiring treatment with MR blockers and potassium supplements. Through this case series of licorice-induced PHA, we aim to increase awareness about exogenous PHA, and the possible risk associated with excess intake of licorice. An accurate history is mandatory in patients with hypertension and hypokalemia to avoid unnecessary testing. GA is a component of several products, such as candies, breath fresheners, beverages, tobacco, cosmetics, and laxatives. In recent years, the mechanisms of action of licorice and its active compounds have been better elucidated, suggesting its benefits in several clinical settings. Nevertheless, licorice should still be consumed with caution, considering that licorice-induced PHA is still an underestimated condition, and its intake should be avoided in patients with increased risk of licorice toxicity due to concomitant comorbidities or interfering drugs. [ABSTRACT FROM AUTHOR]
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- 2024
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166. Effect of mood and worker incentives on workplace productivity†.
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Coviello, Decio, Deserranno, Erika, Persico, Nicola, and Sapienza, Paola
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MOOD (Psychology) ,LABOR productivity ,INDUSTRIAL productivity ,WAGES ,JOB performance - Abstract
We study the causal effect of mood on the productivity of call-center workers. Mood is measured through an online "mood questionnaire" which the workers are encouraged to fill out daily. We find that better mood actually decreases worker productivity for workers whose compensation is largely fixed. The negative effect of mood is attenuated for workers whose compensation is based on performance (high-powered incentives). This finding holds both at a correlational level and in two IV settings, where mood is instrumented for by weather or, alternatively, by whether the local professional sports team played/won the day before. We rule out a number of threats to the exclusion restrictions, and discuss the mechanisms that could generate our findings (JEL J24, J28, M52, C26). [ABSTRACT FROM AUTHOR]
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- 2024
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167. Construction of Partial Differential Equations with Conditional Symmetries
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Levi, Decio, Rodríguez, Miguel A., Thomova, Zora, Feldman, Joel S., Editorial board, Phong, Duong H., Editorial board, Saint-Aubin, Yvan, Editorial Board Member, Vinet, Luc, Editorial Board Member, Kuru, Şengül, editor, Negro, Javier, editor, and Nieto, Luis M., editor
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- 2019
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168. Modelling of the Manipulator of a Mini Hydraulic Excavator
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de Oliveira, Éverton Lins, Donha, Décio Crisol, Fleury, Agenor de T., editor, Rade, Domingos A., editor, and Kurka, Paulo R. G., editor
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- 2019
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169. Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria
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Pâmela Decio, Pinar Ustaoglu, Kamila Derecka, Ian C. W. Hardy, Thaisa C. Roat, Osmar Malaspina, Nigel Mongan, Reinhard Stöger, and Matthias Soller
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Medicine ,Science - Abstract
Abstract Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.
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- 2021
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170. NR5A2/LRH-1 regulates the PTGS2-PGE2-PTGER1 pathway contributing to pancreatic islet survival and function
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Eugenia Martin Vázquez, Nadia Cobo-Vuilleumier, Raquel Araujo Legido, Sandra Marín-Cañas, Emanuele Nola, Akaitz Dorronsoro, Lucia López Bermudo, Alejandra Crespo, Silvana Y. Romero-Zerbo, Maria García-Fernández, Alejandro Martin Montalvo, Anabel Rojas, Valentine Comaills, Francisco J. Bérmudez-Silva, Maureen Gannon, Franz Martin, Decio Eizirik, Petra I. Lorenzo, and Benoit R. Gauthier
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Physiology ,Molecular biology ,Science - Abstract
Summary: LRH-1/NR5A2 is implicated in islet morphogenesis postnatally, and its activation using the agonist BL001 protects islets against apoptosis, reverting hyperglycemia in mouse models of Type 1 Diabetes Mellitus. Islet transcriptome profiling revealed that the expression of PTGS2/COX2 is increased by BL001. Herein, we sought to define the role of LRH-1 in postnatal islet morphogenesis and chart the BL001 mode of action conferring beta cell protection. LRH-1 ablation within developing beta cells impeded beta cell proliferation, correlating with mouse growth retardation, weight loss, and hypoglycemia leading to lethality. LRH-1 deletion in adult beta cells abolished the BL001 antidiabetic action, correlating with beta cell destruction and blunted Ptgs2 induction. Islet PTGS2 inactivation led to reduced PGE2 levels and loss of BL001 protection against cytokines as evidenced by increased cytochrome c release and cleaved-PARP. The PTGER1 antagonist—ONO-8130—negated BL001-mediated islet survival. Our results define the LRH-1/PTGS2/PGE2/PTGER1 signaling axis as a key pathway mediating BL001 survival properties.
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- 2022
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171. The role of immune and oxidative pathways in menstrual cycle associated depressive, physio-somatic, breast and anxiety symptoms: Modulation by sex hormones
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Roomruangwong, Chutima, Matsumoto, Andressa Keiko, Michelin, Ana Paula, de Oliveira Semeão, Laura, de Lima Pedrão, João Victor, Moreira, Estefania G., Sirivichayakul, Sunee, Carvalho, Andre, Barbosa, Decio S., and Maes, Michael
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- 2020
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172. Immune response to purified protein derivative in infants from helminth-sensitized mothers — A cases series
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de Souza Fernandes, Erica, Correia do Nascimento, Wheverton Ricardo, Alves dos Santos, Patrícia, de Oliveira Nóbrega, Cassia Gisele, Barros Lorena, Virginia, Simões Barbosa, Constança, Medeiros, Décio, Wanick Sarinho, Silvia, Assis Costa, Vlaudia Maria, and Oliveira de Souza, Valdênia Maria
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- 2020
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173. Cellular immune response of asthmatic children in the presence of anti-Ascaris antibody
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Nóbrega, Cassia, Nascimento, Wheverton, Lorena, Virgínia, Medeiros, Décio, Costa, Vláudia, Albuquerque, Mônica, Barbosa, Constança, Solé, Dirceu, Sarinho, Emanuel, and Souza, Valdênia
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- 2020
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174. Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention
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Nakayasu, Ernesto S., Syed, Farooq, Tersey, Sarah A., Gritsenko, Marina A., Mitchell, Hugh D., Chan, Chi Yuet, Dirice, Ercument, Turatsinze, Jean-Valery, Cui, Yi, Kulkarni, Rohit N., Eizirik, Decio L., Qian, Wei-Jun, Webb-Robertson, Bobbie-Jo M., Evans-Molina, Carmella, Mirmira., Raghavendra G., and Metz, Thomas O.
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- 2020
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175. High order multiscale analysis of discrete integrable equations
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Hernandez Heredero, Rafael, primary, Levi, Decio, additional, and Scimiterna, Christian, additional
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- 2024
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176. First Evidence of Mineralocorticoid Receptor Gene and Protein Expression in Rat and Human Thyroid Tissues and Cell Cultures
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Manso, Jacopo, primary, Pedron, Maria Chiara, additional, Mondin, Alberto, additional, Censi, Simona, additional, Pennelli, Gianmaria, additional, Galuppini, Francesca, additional, Barollo, Susi, additional, Bertazza, Loris, additional, Radu, Claudia Maria, additional, Ghini, Francesca, additional, Simioni, Paolo, additional, Sabbadin, Chiara, additional, Ceccato, Filippo, additional, Armanini, Decio, additional, and Mian, Caterina, additional
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- 2024
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177. Brazilian Guidelines for In-office and Out-of-office Blood Pressure Measurement – 2023
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Feitosa, Audes Diogenes de Magalhães, primary, Barroso, Weimar Kunz Sebba, additional, Mion Junior, Decio, additional, Nobre, Fernando, additional, Mota-Gomes, Marco Antonio, additional, Jardim, Paulo Cesar Brandão Veiga, additional, Amodeo, Celso, additional, Oliveira, Adriana Camargo, additional, Alessi, Alexandre, additional, Sousa, Ana Luiza Lima, additional, Brandão, Andréa Araujo, additional, Pio-Abreu, Andrea, additional, Sposito, Andrei C., additional, Pierin, Angela Maria Geraldo, additional, Paiva, Annelise Machado Gomes de, additional, Spinelli, Antonio Carlos de Souza, additional, Machado, Carlos Alberto, additional, Poli-de-Figueiredo, Carlos Eduardo, additional, Rodrigues, Cibele Isaac Saad, additional, Forjaz, Claudia Lucia de Moraes, additional, Sampaio, Diogo Pereira Santos, additional, Barbosa, Eduardo Costa Duarte, additional, Freitas, Elizabete Viana de, additional, Cestario, Elizabeth do Espirito Santo, additional, Muxfeldt, Elizabeth Silaid, additional, Lima Júnior, Emilton, additional, Campana, Erika Maria Gonçalves, additional, Feitosa, Fabiana Gomes Aragão Magalhães, additional, Consolim-Colombo, Fernanda Marciano, additional, Almeida, Fernando Antônio de, additional, Silva, Giovanio Vieira da, additional, Moreno Júnior, Heitor, additional, Finimundi, Helius Carlos, additional, Guimarães, Isabel Cristina Britto, additional, Gemelli, João Roberto, additional, Barreto-Filho, José Augusto Soares, additional, Vilela-Martin, José Fernando, additional, Ribeiro, José Marcio, additional, Yugar-Toledo, Juan Carlos, additional, Magalhães, Lucélia Batista Neves Cunha, additional, Drager, Luciano F., additional, Bortolotto, Luiz Aparecido, additional, Alves, Marco Antonio de Melo, additional, Malachias, Marcus Vinícius Bolívar, additional, Neves, Mario Fritsch Toros, additional, Santos, Mayara Cedrim, additional, Dinamarco, Nelson, additional, Moreira Filho, Osni, additional, Passarelli Júnior, Oswaldo, additional, Vitorino, Priscila Valverde de Oliveira, additional, Miranda, Roberto Dischinger, additional, Bezerra, Rodrigo, additional, Pedrosa, Rodrigo Pinto, additional, Paula, Rogerio Baumgratz de, additional, Okawa, Rogério Toshiro Passos, additional, Póvoa, Rui Manuel dos Santos, additional, Fuchs, Sandra C., additional, Lima, Sandro Gonçalves de, additional, Inuzuka, Sayuri, additional, Ferreira-Filho, Sebastião Rodrigues, additional, Fillho, Silvio Hock de Paffer, additional, Jardim, Thiago de Souza Veiga, additional, Guimarães Neto, Vanildo da Silva, additional, Koch, Vera Hermina Kalika, additional, Gusmão, Waléria Dantas Pereira, additional, Oigman, Wille, additional, and Nadruz Junior, Wilson, additional
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- 2024
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178. Diretrizes Brasileiras de Medidas da Pressão Arterial Dentro e Fora do Consultório – 2023
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Feitosa, Audes Diogenes de Magalhães, primary, Barroso, Weimar Kunz Sebba, additional, Mion Junior, Decio, additional, Nobre, Fernando, additional, Mota-Gomes, Marco Antonio, additional, Jardim, Paulo Cesar Brandão Veiga, additional, Amodeo, Celso, additional, Oliveira, Adriana Camargo, additional, Alessi, Alexandre, additional, Sousa, Ana Luiza Lima, additional, Brandão, Andréa Araujo, additional, Pio-Abreu, Andrea, additional, Sposito, Andrei C., additional, Pierin, Angela Maria Geraldo, additional, Paiva, Annelise Machado Gomes de, additional, Spinelli, Antonio Carlos de Souza, additional, Machado, Carlos Alberto, additional, Poli-de-Figueiredo, Carlos Eduardo, additional, Rodrigues, Cibele Isaac Saad, additional, Forjaz, Claudia Lucia de Moraes, additional, Sampaio, Diogo Pereira Santos, additional, Barbosa, Eduardo Costa Duarte, additional, Freitas, Elizabete Viana de, additional, Cestario, Elizabeth do Espirito Santo, additional, Muxfeldt, Elizabeth Silaid, additional, Lima Júnior, Emilton, additional, Campana, Erika Maria Gonçalves, additional, Feitosa, Fabiana Gomes Aragão Magalhães, additional, Consolim-Colombo, Fernanda Marciano, additional, Almeida, Fernando Antônio de, additional, Silva, Giovanio Vieira da, additional, Moreno Júnior, Heitor, additional, Finimundi, Helius Carlos, additional, Guimarães, Isabel Cristina Britto, additional, Gemelli, João Roberto, additional, Barreto-Filho, José Augusto Soares, additional, Vilela-Martin, José Fernando, additional, Ribeiro, José Marcio, additional, Yugar-Toledo, Juan Carlos, additional, Magalhães, Lucélia Batista Neves Cunha, additional, Drager, Luciano F., additional, Bortolotto, Luiz Aparecido, additional, Alves, Marco Antonio de Melo, additional, Malachias, Marcus Vinícius Bolívar, additional, Neves, Mario Fritsch Toros, additional, Santos, Mayara Cedrim, additional, Dinamarco, Nelson, additional, Moreira Filho, Osni, additional, Passarelli Júnior, Oswaldo, additional, Vitorino, Priscila Valverde de Oliveira, additional, Miranda, Roberto Dischinger, additional, Bezerra, Rodrigo, additional, Pedrosa, Rodrigo Pinto, additional, Paula, Rogerio Baumgratz de, additional, Okawa, Rogério Toshiro Passos, additional, Póvoa, Rui Manuel dos Santos, additional, Fuchs, Sandra C., additional, Lima, Sandro Gonçalves de, additional, Inuzuka, Sayuri, additional, Ferreira-Filho, Sebastião Rodrigues, additional, Fillho, Silvio Hock de Paffer, additional, Jardim, Thiago de Souza Veiga, additional, Guimarães Neto, Vanildo da Silva, additional, Koch, Vera Hermina Kalika, additional, Gusmão, Waléria Dantas Pereira, additional, Oigman, Wille, additional, and Nadruz Junior, Wilson, additional
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- 2024
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179. BRAZILIAN CLINICAL GUIDELINE FOR THE THERAPEUTIC MANAGEMENT OF GASTROESOPHAGEAL REFLUX DISEASE (BRAZILIAN FEDERATION OF GASTROENTEROLOGY, FBG)
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MORAES-FILHO, Joaquim Prado P, primary, DOMINGUES, Gerson, additional, and CHINZON, Decio, additional
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- 2024
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180. YIPF5 mutations cause neonatal diabetes and microcephaly through endoplasmic reticulum stress
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De Franco, Elisa, Lytrivi, Maria, Ibrahim, Hazem, Montaser, Hossam, Wakeling, Matthew N., Fantuzzi, Federica, Patel, Kashyap, Demarez, Celine, Cai, Ying, Igoillo-Esteve, Mariana, Cosentino, Cristina, Lithovius, Vaino, Vihinen, Helena, Jokitalo, Eija, Laver, Thomas W., Johnson, Matthew B., Sawatani, Toshiaki, Shakeri, Hadis, Pachera, Nathalie, Haliloglu, Belma, Ozbek, Mehmet Nuri, Unal, Edip, Yildirim, Ruken, Godbole, Tushar, Yildiz, Melek, Aydin, Banu, Bilheu, Angeline, Suzuki, Ikuo, Flanagan, Sarah E., Vanderhaeghen, Pierre, Senee, Valerie, Julier, Cecile, Marchetti, Piero, Eizirik, Decio L., Ellard, Sian, Saarimaki-Vire, Jonna, Otonkoski, Timo, Cnop, Miriam, and Hattersley, Andrew T.
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Pediatric research ,Diabetes mellitus -- Genetic aspects -- Causes of -- Development and progression ,Microcephaly -- Genetic aspects -- Causes of -- Development and progression ,Stress (Physiology) -- Genetic aspects -- Health aspects ,Neonatal diseases -- Genetic aspects -- Causes of -- Development and progression ,Endoplasmic reticulum -- Physiological aspects -- Health aspects ,Health care industry - Abstract
Neonatal diabetes is caused by single gene mutations reducing pancreatic [beta] cell number or impairing [beta] cell function. Understanding the genetic basis of rare diabetes subtypes highlights fundamental biological processes in [beta] cells. We identified 6 patients from 5 families with homozygous mutations in the YIPF5 gene, which is involved in trafficking between the endoplasmic reticulum (ER) and the Golgi. All patients had neonatal/early-onset diabetes, severe microcephaly, and epilepsy. YIPF5 is expressed during human brain development, in adult brain and pancreatic islets. We used 3 human [beta] cell models (YIPF5 silencing in EndoC-[beta]H1 cells, YIPF5 knockout and mutation knockin in embryonic stem cells, and patient-derived induced pluripotent stem cells) to investigate the mechanism through which YIPF5 loss of function affects [beta] cells. Loss of YIPF5 function in stem cell-derived islet cells resulted in proinsulin retention in the ER, marked ER stress, and p cell failure. Partial YIPF5 silencing in EndoC-[beta]H1 cells and a patient mutation in stem cells increased the [beta] cell sensitivity to ER stress-induced apoptosis. We report recessive YIPF5 mutations as the genetic cause of a congenital syndrome of microcephaly, epilepsy, and neonatal/early-onset diabetes, highlighting a critical role of YIPF5 in [beta] cells and neurons. We believe this is the first report of mutations disrupting the ER-to-Golgi trafficking, resulting in diabetes., Introduction Neonatal diabetes mellitus develops before 6 months of age and is caused by reduced pancreatic [beta] cell number (reduced formation/increased destruction) or impaired [beta] cell function. Previous studies have [...]
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- 2020
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181. Gestão de Riscos Ocupacionais: técnicas para o processo de avaliação de riscos
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Heidtmann-Bemvenuti, Renata, primary, Decio, Andria Lemos Huelsen, additional, Cruz, Carolina Paz da, additional, and Franz, Luis Antonio dos Santos, additional
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- 2021
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182. Complex association between post-COVID-19 condition and anxiety and depression symptoms
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Tebeka, Sarah, primary, Carcaillon-Bentata, Laure, additional, Decio, Valentina, additional, Alleaume, Caroline, additional, Beltzer, Nathalie, additional, Gallay, Anne, additional, Lemogne, Cédric, additional, Pignon, Baptiste, additional, Makovski, Tatjana T., additional, and Coste, Joël, additional
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- 2023
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183. Development of a HPLC-MS/MS Method to Assess the Pharmacokinetics and Tumour Distribution of the Dimethylarginine Dimethylaminohydrolase 1 Inhibitors ZST316 and L-257 in a Xenograft Model of Triple-Negative Breast Cancer in Mice
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Ceruti, Tommaso, primary, Frapolli, Roberta, additional, Ghilardi, Carmen, additional, Decio, Alessandra, additional, Dellavedova, Giulia, additional, Tommasi, Sara, additional, Zucchetti, Massimo, additional, and Mangoni, Arduino A., additional
- Published
- 2023
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184. Fiber Optical Cable and Connector System (FOCCoS) for PFS/Subaru
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de Oliveira, Antonio Cesar, de Oliveira, Lígia Souza, de Arruda, Márcio V., Marrara, Lucas Souza, Santos, Leandro H. dos, Ferreira, Décio, Santos, Jesulino B. dos, Rosa, Josimar A., Junior, Orlando V., Pereira, Jeferson M., Castilho, Bruno, Gneiding, Clemens, Junior, Laerte S., de Oliveira, Claudia M., Gunn, James E., Ueda, Akitoshi, Takato, Naruhisa, Shimono, Atsushi, Sugai, Hajime, Karoji, Hiroshi, Kimura, Masahiko, Tamura, Naoyuki, Wang, Shiang-Yu, Murray, Graham, Mignant, David Le, Madec, Fabrice, Jaquet, Marc, Vives, Sebastien, Fisher, Charlie, Braun, David, Schwochert, Mark, and Reiley, Daniel J.
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Astrophysics - Instrumentation and Methods for Astrophysics - Abstract
FOCCoS, Fiber Optical Cable and Connector System, has the main function of capturing the direct light from the focal plane of Subaru Telescope using optical fibers, each one with a microlens in its tip, and conducting this light through a route containing connectors to a set of four spectrographs. The optical fiber cable is divided in 3 different segments called Cable A, Cable B and Cable C. Multi-fibers connectors assure precise connection among all optical fibers of the segments, providing flexibility for instrument changes. To assure strong and accurate connection, these sets are arranged inside two types of assemblies: the Tower Connector, for connection between Cable C and Cable B; and the Gang Connector, for connection between Cable B and Cable A. Throughput tests were made to evaluate the efficiency of the connections. A lifetime test connection is in progress. Cable C is installed inside the PFI, Prime Focus Instrument, where each fiber tip with a microlens is bonded to the end of the shaft of a 2-stage piezo-electric rotatory motor positioner; this assembly allows each fiber to be placed anywhere within its patrol region, which is 9.5mm diameter.. Each positioner uses a fiber arm to support the ferrule, the microlens, and the optical fiber. 2400 of these assemblies are arranged on a motor bench plate in a hexagonal-closed-packed disposition., Comment: 11 pages, 20 figures
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- 2014
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185. Multi-fibers connectors systems for FOCCoS-PFS-Subaru
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de Oliveira, Antonio Cesar, de Oliveira, Ligia Souza, Marrara, Lucas Souza, Santos, Leandro Henrique dos, de Arruda, Marcio Vital, Santos, Jesulino Bispo dos, Ferreira, Décio, Rosa, Josimar Aparecido, Vilaca, Rodrigo de Paiva, de Oliveira, Laerte Sodre Junior Claudia Mendes, and Gunn, James E.
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Astrophysics - Instrumentation and Methods for Astrophysics - Abstract
The Fiber Optical Cable and Connector System (FOCCoS), provides optical connection between 2400 positioners and a set of spectrographs through optical fibers cables as part of PFS instrument for Subaru telescope. The optical fiber cable will be segmented in 3 parts along the route, cable A, cable B and cable C, connected by a set of multi-fiber connectors. The company USCONEC produces the multi-fiber connector under study. The USCONEC 32F model can connect 32 optical fibers in a 4 x 8 matrix arrangement. The ferrules are made of a durable composite, Polyphenylene Sulfide (PPS) based thermoplastic. The connections are held in place by a push-on/pull-off latch, and the connector can also be distinguished by a pair of metal guide pins that protrude from the front of the connector. Two fibers per connector will be used for monitoring the connection procedure. It was found to be easy to polish and it is small enough to be mounted in groups. Highly multiplexed instruments like PFS require a fiber connector system that can deliver excellent optical performance and reliability. PFS requires two different types of structures to organize the connectors. The Tower Connector system, with 80 multi-fiber connectors, will be a group of connectors for connecting cable B (Telescope Structure) with cable C (Positioners Plate). The Gang Connector system is a group of 8 gang connectors, each one with 12 multi-fibers connectors, for connecting cable B (Telescope Structure) with cable A (Spectrograph). The bench tests with these connector systems and the chosen fibers should measure the throughput of light and the stability after many connections and disconnections. In this paper we describe tests and procedures to evaluate the throughput and FRD increment. The lifetime of the ferrules is also in evaluation.
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- 2014
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186. Polish device for FOCCoS/PFS slit system
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de Oliveira, Antonio Cesar, de Oliveira, Ligia Souza, de Arruda, Marcio Vital, Marrara, Lucas Souza, Santos, Leandro Henrique dos, Ferreira, Décio, Santos, Jesulino Bispo dos, Vilaca, Rodrigo de Paiva, Rosa, Josimar Aparecido, Junior, Laerte Sodre, and de Oliveira, Claudia Mendes
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Astrophysics - Instrumentation and Methods for Astrophysics ,Physics - Instrumentation and Detectors - Abstract
The Fiber Optical Cable and Connector System, FOCCoS, for the Prime Focus Spectrograph, PFS, is responsible for transporting light from the Subaru Telescope focal plane to a set of four spectrographs. Each spectrograph will be fed by a convex curved slit with 616 optical fibers organized in a linear arrangement. The slit frontal surface is covered with a special dark composite, made with refractory oxide, which is able to sustain its properties with minimum quantities of abrasives during the polishing process; this stability is obtained This stability is obtained by the detachment of the refractory oxide nanoparticles, which then gently reinforce gently the polishing process and increase its the efficiency. The surface roughness measured in several samples after high performance polishing was about 0.01 microns. Furthermore, the time for obtaining a polished surface with this quality is about 10 times less than the time required for polishing a brass, glass or ceramic surface of the same size. In this paper, we describe the procedure developed for high quality polishing of this type of slit. The cylindrical polishing described here, uses cylindrical concave metal bases on which glass paper is based. The polishing process consists to use grid sequences of 30 microns, 12 microns, 9 microns, 5 microns, 3 microns, 1 micron and, finally, a colloidal silica on a chemical cloth. To obtain the maximum throughput, the surface of the fibers should be polished in such a way that they are optically flat and free from scratches. The optical fibers are inspected with a microscope at all stages of the polishing process to ensure high quality. The efficiency of the process may be improved by using a cylindrical concave composite base as a substrate suitable for diamond liquid solutions. Despite this process being completely by hand, the final result shows a very high quality.
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- 2014
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187. Slit device for FOCCoS, PFS, Subaru
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de Oliveira, Antonio Cesar, Gunn, James E., de Oliveira, Ligia Souza, de Arruda, Marcio Vital, Marrara, Lucas Souza, Santos, Leandro Henrique dos, Ferreira, Décio, Santos, Jesulino Bispo dos, Rosa, Josimar Aparecido, Ribeiro, Flavio Felipe, Vilaça, Rodrigo de Paiva, Junior, Orlando Verducci, Junior, Laerte Sodré, and de Oliveira, Claudia Mendes
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Astrophysics - Instrumentation and Methods for Astrophysics - Abstract
The Fiber Optical Cable and Connector System, FOCCoS, subsystem of the Prime Focus Spectrograph, PFS, for Subaru telescope, is responsible to feed four spectrographs with a set of optical fibers cables. The light injection for each spectrograph is assured by a convex curved slit with a linear array of 616 optical fibers. In this paper we present a design of a slit that ensures the right direction of the fibers by using masks of micro holes. This kind of mask is made by a technique called electroforming, which is able to produce a nickel plate with holes in a linear sequence. The precision error is around 1micron in the diameter and 1 micron in the positions of the holes. This nickel plate may be produced with a thickness between 50 and 200 microns, so it may be very flexible. This flexibility allows the mask to be bent into the shape necessary for a curved slit. The concept requires two masks, which we call Front Mask, and Rear Mask, separated by a gap that defines the thickness of the slit. The pitch and the diameter of the holes define the linear geometry of the slit; the curvature of each mask defines the angular geometry of the slit. Obviously, this assembly must be mounted inside a structure rigid and strong enough to be supported inside the spectrograph. This structure must have a CTE optimized to avoid displacement of the fibers or increased FRD of the fibers when the device is submitted to temperatures around 3 degrees Celsius, the temperature of operation of the spectrograph. We have produced two models. Both are mounted inside a very compact Invar case, and both have their front surfaces covered by a dark composite, to reduce stray light. Furthermore, we have conducted experiments with two different internal structures to minimize effects caused by temperature gradients.
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- 2014
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188. Progress with the Prime Focus Spectrograph for the Subaru Telescope: a massively multiplexed optical and near-infrared fiber spectrograph
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Sugai, Hajime, Tamura, Naoyuki, Karoji, Hiroshi, Shimono, Atsushi, Takato, Naruhisa, Kimura, Masahiko, Ohyama, Youichi, Ueda, Akitoshi, Aghazarian, Hrand, de Arruda, Marcio Vital, Barkhouser, Robert H., Bennett, Charles L., Bickerton, Steve, Bozier, Alexandre, Braun, David F., Bui, Khanh, Capocasale, Christopher M., Carr, Michael A., Castilho, Bruno, Chang, Yin-Chang, Chen, Hsin-Yo, Chou, Richard C. Y., Dawson, Olivia R., Dekany, Richard G., Ek, Eric M., Ellis, Richard S., English, Robin J., Ferrand, Didier, Ferreira, Décio, Fisher, Charles D., Golebiowski, Mirek, Gunn, James E., Hart, Murdock, Heckman, Timothy M., Ho, Paul T. P., Hope, Stephen, Hovland, Larry E., Hsuc, Shu-Fu, Hu, Yen-Shan, Huang, Pin Jie, Jaquet, Marc, Karr, Jennifer E., Kempenaar, Jason G., King, Matthew E., Fèvre, Olivier Le, Mignant, David Le, Ling, Hung-Hsu, Loomis, Craig, Lupton, Robert H., Madec, Fabrice, Mao, Peter, Marrara, Lucas Souza, Ménard, Brice, Morantz, Chaz, Murayama, Hitoshi, Murray, Graham J., de Oliveira, Antonio Cesar, de Oliveira, Claudia Mendes, de Oliveira, Ligia Souza, Orndorff, Joe D., Vilaça, Rodrigo de Paiva, Partos, Eamon J., Pascal, Sandrine, Pegot-Ogier, Thomas, Reiley, Daniel J., Riddle, Reed, Santos, Leandro, Santos, Jesulino Bispo dos, Schwochert, Mark A., Seiffert, Michael D., Smee, Stephen A., Smith, Roger M., Steinkraus, Ronald E., Sodré Jr, Laerte, Spergel, David N., Surace, Christian, Tresse, Laurence, Vidal, Clément, Vives, Sebastien, Wang, Shiang-Yu, Wen, Chih-Yi, Wu, Amy C., Wyse, Rosie, and Yan, Chi-Hung
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Astrophysics - Instrumentation and Methods for Astrophysics - Abstract
The Prime Focus Spectrograph (PFS) is an optical/near-infrared multi-fiber spectrograph with 2394 science fibers, which are distributed in 1.3 degree diameter field of view at Subaru 8.2-meter telescope. The simultaneous wide wavelength coverage from 0.38 um to 1.26 um, with the resolving power of 3000, strengthens its ability to target three main survey programs: cosmology, Galactic archaeology, and galaxy/AGN evolution. A medium resolution mode with resolving power of 5000 for 0.71 um to 0.89 um also will be available by simply exchanging dispersers. PFS takes the role for the spectroscopic part of the Subaru Measurement of Images and Redshifts project, while Hyper Suprime-Cam works on the imaging part. To transform the telescope plus WFC focal ratio, a 3-mm thick broad-band coated glass-molded microlens is glued to each fiber tip. A higher transmission fiber is selected for the longest part of cable system, while one with a better FRD performance is selected for the fiber-positioner and fiber-slit components, given the more frequent fiber movements and tightly curved structure. Each Fiber positioner consists of two stages of piezo-electric rotary motors. Its engineering model has been produced and tested. Fiber positioning will be performed iteratively by taking an image of artificially back-illuminated fibers with the Metrology camera located in the Cassegrain container. The camera is carefully designed so that fiber position measurements are unaffected by small amounts of high special-frequency inaccuracies in WFC lens surface shapes. Target light carried through the fiber system reaches one of four identical fast-Schmidt spectrograph modules, each with three arms. Prototype VPH gratings have been optically tested. CCD production is complete, with standard fully-depleted CCDs for red arms and more-challenging thinner fully-depleted CCDs with blue-optimized coating for blue arms., Comment: 14 pages, 12 figures, submitted to "Ground-based and Airborne Instrumentation for Astronomy V, Suzanne K. Ramsay, Ian S. McLean, Hideki Takami, Editors, Proc. SPIE 9147 (2014)"
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- 2014
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189. Lie-point symmetries of the discrete Liouville equation
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Levi, Decio, Martina, Luigi, and Winternitz, Pavel
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Mathematical Physics ,Mathematics - Numerical Analysis ,Nonlinear Sciences - Exactly Solvable and Integrable Systems - Abstract
The Liouville equation is well known to be linearizable by a point transformation. It has an infinite dimensional Lie point symmetry algebra isomorphic to a direct sum of two Virasoro algebras. We show that it is not possible to discretize the equation keeping the entire symmetry algebra as point symmetries. We do however construct a difference system approximating the Liouville equation that is invariant under the maximal finite subalgebra $ SL_x \lf 2 , \mathbb{R} \rg \otimes SL_y \lf 2 , \mathbb{R} \rg $. The invariant scheme is an explicit one and provides a much better approximation of exact solutions than comparable standard (non invariant) schemes.
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- 2014
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190. On the construction of partial difference schemes II: discrete variables and Schwarzian lattices
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Levi, Decio and Rodriguez, Miguel A.
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Mathematical Physics ,Mathematics - Numerical Analysis ,39A14, 35F05 - Abstract
In the process of constructing invariant difference schemes which approximate partial differential equations we write down a procedure for discretizing an arbitrary partial differential equation on an arbitrary lattice. An open problem is the meaning of a lattice which does not satisfy the Clairaut--Schwarz--Young theorem. To analyze it we apply the procedure on a simple example, the potential Burgers equation with two different lattices, an orthogonal lattice which is invariant under the symmetries of the equation and satisfies the commutativity of the partial difference operators and an exponential lattice which is not invariant and does not satisfy the Clairaut--Schwarz--Young theorem. A discussion on the numerical results is also presented showing the different behavior of both schemes for two different exact solutions and their numerical approximations., Comment: 14 pages, 4 figures
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- 2014
191. Potentiality and Contradiction in Quantum Mechanics
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Arenhart, Jonas R. B. and Krause, Décio
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Quantum Physics - Abstract
Following J.-Y.B\'eziau in his pioneer work on non-standard interpretations of the traditional square of opposition, we have applied the abstract structure of the square to study the relation of opposition between states in superposition in orthodox quantum mechanics in \cite{are14}. Our conclusion was that such states are \ita{contraries} (\ita{i.e.} both can be false, but both cannot be true), contradicting previous analyzes that have led to different results, such as those claiming that those states represent \ita{contradictory} properties (\ita{i. e.} they must have opposite truth values). In this chapter we bring the issue once again into the center of the stage, but now discussing the metaphysical presuppositions which underlie each kind of analysis and which lead to each kind of result, discussing in particular the idea that superpositions represent potential contradictions. We shall argue that the analysis according to which states in superposition are contrary rather than contradictory is still more plausible.
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- 2014
192. Contradiction, Quantum Mechanics, and the Square of Opposition
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Arenhart, Jonas R. B. and Krause, Décio
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Quantum Physics - Abstract
We discuss the idea that superpositions in quantum mechanics may involve contradictions or contradictory properties. A state of superposition such as the one comprised in the famous Schr\"odinger's cat, for instance, is sometimes said to attribute contradictory properties to the cat: being dead and alive at the same time. If that were the case, we would be facing a revolution in logic and science, since we would have one of our greatest scientific achievements showing that real contradictions exist. We analyze that claim by employing the traditional square of opposition. We suggest that it is difficult to make sense of the idea of contradiction in the case of quantum superpositions. From a metaphysical point of view the suggestion also faces obstacles, and we present some of them.
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- 2014
193. Lie group analysis of a generalized Krichever-Novikov differential-difference equation
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Levi, Decio, Ricca, Eugenio, Thomova, Zora, and Winternitz, Pavel
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Nonlinear Sciences - Exactly Solvable and Integrable Systems ,Mathematical Physics ,Mathematics - Group Theory - Abstract
The symmetry algebra of the differential--difference equation $$\dot u_n = [P(u_n)u_{n+1}u_{n-1} + Q(u_n)(u_{n+1}+u_{n-1})+ R(u_n)]/(u_{n+1}-u_{n-1}),$$ where $P$, $Q$ and $R$ are arbitrary analytic functions is shown to have the dimension $1 \le \mbox{dim}L \le 5$. When $P$, $Q$ and $R$ are specific second order polynomials in $u_n$ (depending on 6 constants) this is the integrable discretization of the Krichever--Novikov equation. We find 3 cases when the arbitrary functions are not polynomials and the symmetry algebra satisfies $\mbox{dim}L=2$. These cases are shown not to be integrable. The symmetry algebras are used to reduce the equations to purely difference ones. The symmetry group is also used to impose periodicity $u_{n+N}=u_n$ and thus to reduce the differential--difference equation to a system of $N$ coupled ordinary three points difference equations.
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- 2014
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194. Idiopathic inflammatory myopathies and hypertension: Possible involvement of hormonal factors
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Decio Armanini, Chiara Sabbadin, and Luciana Bordin
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aldosterone ,cortisol ,glucocorticoids ,hypertension ,idiopathic inflammatory myopathies ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2021
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195. SUMOylation of Pdia3 exacerbates proinsulin misfolding and ER stress in pancreatic beta cells
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Li, Na, Luo, Xi, Yu, Qilin, Yang, Ping, Chen, Zhishui, Wang, Xinqiang, Jiang, Jipin, Xu, Jing, Gong, Quan, Eizirik, Decio L., Zhou, Zhiguang, Zhao, Jiajun, Xiong, Fei, Zhang, Shu, and Wang, Cong-Yi
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- 2020
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196. Indistinguishability and the origins of contextuality in physics
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de Barros, J. Acacio, Holik, Federico, and Krause, Décio
- Published
- 2019
197. Coffee researches: Pesquisa em café (versão em inglês-English version)
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Decio Zylbersztajn, Samuel Ribeiro Giordano, Christiane Leles Rezende
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- 2021
198. Combined transcriptome and proteome profiling of the pancreatic β-cell response to palmitate unveils key pathways of β-cell lipotoxicity
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Maria Lytrivi, Kassem Ghaddar, Miguel Lopes, Victoria Rosengren, Anthony Piron, Xiaoyan Yi, Henrik Johansson, Janne Lehtiö, Mariana Igoillo-Esteve, Daniel A. Cunha, Lorella Marselli, Piero Marchetti, Henrik Ortsäter, Decio L. Eizirik, and Miriam Cnop
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Pancreatic islets ,Beta-cells ,Free fatty acids ,Lipid metabolism ,Endoplasmic reticulum stress ,RNA-sequencing ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Prolonged exposure to elevated free fatty acids induces β-cell failure (lipotoxicity) and contributes to the pathogenesis of type 2 diabetes. In vitro exposure of β-cells to the saturated free fatty acid palmitate is a valuable model of lipotoxicity, reproducing features of β-cell failure observed in type 2 diabetes. In order to map the β-cell response to lipotoxicity, we combined RNA-sequencing of palmitate-treated human islets with iTRAQ proteomics of insulin-secreting INS-1E cells following a time course exposure to palmitate. Results Crossing transcriptome and proteome of palmitate-treated β-cells revealed 85 upregulated and 122 downregulated genes at both transcript and protein level. Pathway analysis identified lipid metabolism, oxidative stress, amino-acid metabolism and cell cycle pathways among the most enriched palmitate-modified pathways. Palmitate induced gene expression changes compatible with increased free fatty acid mitochondrial import and β-oxidation, decreased lipogenesis and modified cholesterol transport. Palmitate modified genes regulating endoplasmic reticulum (ER) function, ER-to-Golgi transport and ER stress pathways. Furthermore, palmitate modulated cAMP/protein kinase A (PKA) signaling, inhibiting expression of PKA anchoring proteins and downregulating the GLP-1 receptor. SLC7 family amino-acid transporters were upregulated in response to palmitate but this induction did not contribute to β-cell demise. To unravel critical mediators of lipotoxicity upstream of the palmitate-modified genes, we identified overrepresented transcription factor binding sites and performed network inference analysis. These identified LXR, PPARα, FOXO1 and BACH1 as key transcription factors orchestrating the metabolic and oxidative stress responses to palmitate. Conclusions This is the first study to combine transcriptomic and sensitive time course proteomic profiling of palmitate-exposed β-cells. Our results provide comprehensive insight into gene and protein expression changes, corroborating and expanding beyond previous findings. The identification of critical drivers and pathways of the β-cell lipotoxic response points to novel therapeutic targets for type 2 diabetes.
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- 2020
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199. Pro-inflammatory cytokines induce cell death, inflammatory responses, and endoplasmic reticulum stress in human iPSC-derived beta cells
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Stéphane Demine, Andrea Alex Schiavo, Sandra Marín-Cañas, Piero Marchetti, Miriam Cnop, and Decio L. Eizirik
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Pancreatic beta cells ,Type 1 diabetes ,Induced pluripotent stem cells ,Cytokines ,Apoptosis ,Endoplasmic reticulum stress ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Adult human pancreatic beta cells are the “gold standard” for studies on diabetes pathogenesis, but their use is limited by insufficient availability and variable quality. An important effort has recently taken place to differentiate beta cells from human induced pluripotent stem cells (iPSCs) and validate their use for diabetes research. We presently used a 7-stage protocol to generate beta cells from human iPSC and evaluated whether these cells are responsive to the pro-inflammatory cytokines (IFNγ, IL-1β, or IFNα) that play a role in type 1 diabetes. Methods The iPSC-derived islet-like cell clusters contained 40–50% beta and 10–15% alpha cells and expressed the receptors for IFNγ, IL-1β, or IFNα. Cells were exposed to either IFNγ (1000 U/mL) + IL-1β (50 U/mL) or IFNα alone (2000 U/mL) for 24/48 h. Apoptosis was quantified using Hoechst/propidium iodide staining or the RealTime Glo Apoptosis Kit (Promega). After treatment, CXCL10 secretion was quantified by ELISA. The expression of multiples genes (Ins, Gcg, Nkx2.2, Nkx6.1, Pdx1, Mafa, BiP, Chop, Atf3, CXCL10, CXCL9, CCL5, and HLA-ABC) was quantified by RT-qPCR. Phosphorylation state and total expression of STAT1/STAT2, as well as expression of PDL1 and of the ER chaperone BiP, were quantified by Western blotting. The co-localization of HLA-ABC or cleaved caspase-3 and Ins/Gcg expression was assessed by immunohistochemistry. The presence of HLA-ABC at the plasma membrane was measured by flow cytometry. Results IFNγ + IL-1β and IFNα induced apoptosis of the cells after 48 h of exposure. Cleaved caspase-3 co-localized mostly but not exclusively with Ins+ cells. Exposure to IFNγ + IL-1β induced a pro-inflammatory phenotype, including increased CXCL10, CXCL9, and CCL5 expression; CXCL10 secretion; and HLA-ABC expression. HLA overexpression was confirmed at the protein level by Western blotting and flow cytometry. Exposure to IFNγ + IL-1β (but not IFNα) also induced beta cell dedifferentiation and endoplasmic reticulum stress (increase in BiP, Chop, and Atf3 mRNA expression). Phosphorylation of STAT1 was stimulated already after 1 h by IFNγ + IL-1β and IFNα, while phosphorylation of STAT2 was only activated by IFNα at 1–4 h. PDL1 expression was increased by both IFNγ + IL-1β and IFNα. Conclusions Our data show that human iPSC-derived beta cells respond to pro-inflammatory cytokines IL-1β + IFNγ and IFNα, by activating the same pathogenic processes as adult human primary beta cells. These cells thus represent a valuable tool for future research on the pathogenesis of type 1 diabetes.
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- 2020
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200. Hospitalization for COVID-19 is associated with a higher risk of subsequent hospitalization for psychiatric disorders: A French nationwide longitudinal study comparing hospitalizations for COVID-19 and for other reasons
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Valentina Decio, Philippe Pirard, Baptiste Pignon, Olivier Bouaziz, Vittorio Perduca, Francis Chin, Yann Le Strat, Jonathan Messika, Viviane Kovess-Masfety, Emmanuelle Corruble, Nolwenn Regnault, and Sarah Tebeka
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COVID-19 ,epidemiology ,hospitalization ,psychiatric disorders ,SNDS ,Psychiatry ,RC435-571 - Abstract
Abstract Introduction Although COVID-19 has been associated with psychiatric symptoms in patients, no study to date has examined the risk of hospitalization for psychiatric disorders after hospitalization for this disease. Objective We aimed to compare the proportions of hospitalizations for psychiatric disorders in the 12 months following either hospitalization for COVID-19 or hospitalization for another reason in the adult general population in France during the first wave of the current pandemic. Methods We conducted a retrospective longitudinal nationwide study based on the national French administrative healthcare database. Results Among the 2,894,088 adults hospitalized, 96,313 (3.32%) were admitted for COVID-19. The proportion of patients subsequently hospitalized for a psychiatric disorder was higher for COVID-19 patients (11.09 vs. 9.24%, OR = 1.20 95%CI 1.18–1.23). Multivariable analyses provided similar results for a psychiatric disorder of any type and for psychotic and anxiety disorders (respectively, aOR = 1.06 95%CI 1.04–1.09, aOR = 1.09 95%CI 1.02–1.17, and aOR = 1.11 95%CI 1.08–1.14). Initial hospitalization for COVID-19 in intensive care units and psychiatric history were associated with a greater risk of subsequent hospitalization for any psychiatric disorder than initial hospitalization for another reason. Discussion Compared with hospitalizations for other reasons, hospitalizations for COVID-19 during the first wave of the pandemic in France were associated with a higher risk of hospitalization for a psychiatric disorder during the 12 months following initial discharge. This finding should encourage clinicians to increase the monitoring and assessment of psychiatric symptoms after hospital discharge for COVID-19, and to propose post-hospital care, especially for those treated in intensive care.
- Published
- 2022
- Full Text
- View/download PDF
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