151. Prospective Evaluation of Enzyme-Linked Immunosorbent Assay and Immunoblot Methods for the Diagnosis of Endemic Strongyloides Stercoralis Infection
- Author
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David J. Conway, A.E. Bianco, D. A. P. Bundy, John F. Lindo, N.S. Atkins, and R. D. Robinson
- Subjects
Immunoblotting ,Antibodies, Helminth ,Helminthiasis ,Enzyme-Linked Immunosorbent Assay ,Cross Reactions ,Sensitivity and Specificity ,Strongyloides stercoralis ,Serology ,Feces ,Antigen ,Virology ,medicine ,Animals ,Humans ,Helminths ,False Positive Reactions ,Prospective Studies ,Incubation ,biology ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Strongyloidiasis ,Evaluation Studies as Topic ,Antigens, Helminth ,Immunoglobulin G ,Larva ,biology.protein ,Parasitology ,Onchocerca ,Antibody - Abstract
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80% to 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100%, 85%, and 65%, respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.
- Published
- 1994