Search

Your search keyword '"Crowley, Michael"' showing total 6,929 results
Start Over Author "Crowley, Michael"
6,929 results on '"Crowley, Michael"'

160. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.

Author

Mallinson, Sam JB, Machovina, Melodie M, Silveira, Rodrigo L, Garcia-Borràs, Marc, Gallup, Nathan, Johnson, Christopher W, Allen, Mark D, Skaf, Munir S, Crowley, Michael F, Neidle, Ellen L, Houk, Kendall N, Beckham, Gregg T, DuBois, Jennifer L, and McGeehan, John E

Subjects
Actinobacteria, Lignin, Cytochrome P-450 Enzyme System, Oxidoreductases, O-Demethylating, Bacterial Proteins, Substrate Specificity, Oxidation-Reduction, Protein Multimerization, Oxidoreductases, O-Demethylating
Abstract

Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.

Published
2018

162. Endoluminal Biopsy for Vein of Galen Malformation

Author

Hale, Andrew T., primary, Liu, Shanrun, additional, Huang, Fengyuan, additional, Song, Yuwei, additional, Crowley, Michael R., additional, Crossman, David K., additional, Caudill, Caroline, additional, Arynchyna-Smith, Anastasia, additional, Chapman, Lindsey, additional, Feldman, Michael J., additional, Saccomano, Benjamin W., additional, Rocque, Brandon G., additional, Rozzelle, Curtis J., additional, Blount, Jeffrey P., additional, Johnston, James M., additional, Chong, Zechen, additional, and Jones, Jesse G., additional

Published
2024
Full Text
View/download PDF

163. The Neural Basis of Attentional Blink as a Selective Control Mechanism in Conscious Perception

Author

Xin, Qilong, primary, Forman, Sarit, additional, Christison-Lagay, Kate, additional, Micek, Christopher, additional, Kronemer, Sharif, additional, Aksen, Mark, additional, Grobois, Lauren, additional, Contreras Ramirez, Violeta, additional, Khalaf, Aya, additional, Jin, David, additional, Aerts, Shanae, additional, Chun, Marvin, additional, Crowley, Michael, additional, and Blumenfeld, Hal, additional

Published
2024
Full Text
View/download PDF

165. Australian Attitudes Towards Privacy of Information: Will COVID-19 Make a Difference?

Author

Shanley, Leah, Johnstone, Michael N., Crowley, Michael, Szewczyk, Patryk, Rannenberg, Kai, Editor-in-Chief, Soares Barbosa, Luís, Editorial Board Member, Goedicke, Michael, Editorial Board Member, Tatnall, Arthur, Editorial Board Member, Neuhold, Erich J., Editorial Board Member, Stiller, Burkhard, Editorial Board Member, Tröltzsch, Fredi, Editorial Board Member, Pries-Heje, Jan, Editorial Board Member, Kreps, David, Editorial Board Member, Reis, Ricardo, Editorial Board Member, Furnell, Steven, Editorial Board Member, Mercier-Laurent, Eunika, Editorial Board Member, Winckler, Marco, Editorial Board Member, Malaka, Rainer, Editorial Board Member, and Clarke, Nathan, editor

Published
2020
Full Text
View/download PDF

166. Squeaky Clean: Products to reduce soot and clean up oil unveiled at WorkBoat Show

Author

Crowley, Michael

Subjects
Shipping industry, Global temperature changes, Air pollution, Transportation industry
Abstract

It doesn't matter what publication you are reading, what you're listening to on the radio or what you are watching on the computer screen, it's hard to ignore climate change. [...]

Published
2024

167. Mixed Greens: Hybrid power is appearing in different forms

Author

Crowley, Michael

Subjects
United States. Coast Guard -- Environmental aspects, Hydrogen -- Environmental aspects, Hydrogen as fuel -- Environmental aspects, Transportation industry
Abstract

The use of alternatively powered workboats is growing. Vessel owners and operators, their interest piqued by the prospect of cost-effective, environmentally friendly propulsion, continue to seek new and potentially more [...]

Published
2024

168. Hot Boats: Fireboats are always in demand

Author

Crowley, Michael

Subjects
Fire extinction -- Equipment and supplies, Cigarette boats, Boating industry, Boats and boating, Transportation industry
Abstract

MetalCraft Marine's Bob Clark loves talking about small boats, especially small boats his company builds. 'The one that's really hot is the 43/44,' he said, referring to MetalCraft's 43'1'x144' FireStorm [...]

Published
2024

170. Progressive Power: A peek at nonconventional propulsion choices

Author

Crowley, Michael

Subjects
Diesel motor, Periodical publishing, Transportation industry
Abstract

An indicator of the gathering momentum for cleaner burning, nontraditional forms of propulsion for the U.S. workboat industry can be found in WorkBoat's Significant Boats of 2021. The list of [...]

Published
2022

171. Efficient estimation of the maximum metabolic productivity of batch systems

Author

John, Peter C. St., Crowley, Michael F., and Bomble, Yannick J.

Subjects
Quantitative Biology - Quantitative Methods
Abstract

Production of chemicals from engineered organisms in a batch culture involves an inherent trade-off between productivity, yield, and titer. Existing strategies for strain design typically focus on designing mutations that achieve the highest yield possible while maintaining growth viability. While these methods are computationally tractable, an optimum productivity could be achieved by a dynamic strategy in which the intracellular division of resources is permitted to change with time. New methods for the design and implementation of dynamic microbial processes, both computational and experimental, have therefore been explored to maximize productivity. However, solving for the optimal metabolic behavior under the assumption that all fluxes in the cell are free to vary is a challenging numerical task. This work presents an efficient method for the calculation of a maximum theoretical productivity of a batch culture system using a dynamic optimization framework. This metric is analogous to the maximum theoretical yield, a measure that is well established in the metabolic engineering literature and whose use helps guide strain and pathway selection. The proposed method follows traditional assumptions of dynamic flux balance analysis: (1) that internal metabolite fluxes are governed by a pseudo-steady state, and (2) that external metabolite fluxes are dynamically bounded. The optimization is achieved via collocation on finite elements, and accounts explicitly for an arbitrary number of flux changes. The method can be further extended to explicitly solve for the trade-off curve between maximum productivity and yield. We demonstrate the method on succinate production in two common microbial hosts, Escherichia coli and Actinobacillus succinogenes, revealing that nearly optimal yields and productivities can be achieved with only two discrete flux stages.

Published
2016

185. U.S. Campaign to Isolate Russia Hasn't Had Effect Washington Had Hoped

Author

Wong, Edward and Crowley, Michael

Subjects
Russia -- International economic relations, Brazil -- International economic relations, China -- International economic relations, India -- International economic relations, Russian Invasion of Ukraine, 2022-, Economic sanctions, American, Sanctions (International law) -- Laws, regulations and rules, Presidents -- Foreign policy -- Military policy, Government regulation, General interest, News, opinion and commentary
Abstract

Many nations insist on not taking sides in the war in Ukraine, while China, India and Brazil are filling Russia's coffers. The Biden administration and European allies call President Vladimir [...]

Published
2024

186. U.S. Designates Houthis a Terrorist Group

Author

Crowley, Michael

Subjects
United States. Department of State, Terrorism, Terrorist organizations, News, opinion and commentary
Abstract

The designation allows the United States to crack down on the group’s access to the global financial system. The State Department on Friday designated the Houthis as a terrorist organization, [...]

Published
2024

187. U.S. Rejects Russia's Latest Request for Talks on War

Author

Crowley, Michael

Subjects
Russian Invasion of Ukraine, 2022-, Diplomatic negotiations in international disputes, Military assistance, American -- Political aspects, Pacific settlement of international disputes, Presidents -- Political activity -- Foreign policy, General interest, News, opinion and commentary
Abstract

Skepticism remains high about the Russian leader's intentions after he told Tucker Carlson that the war in Ukraine could be settled with a peace deal. The Biden administration dismissed on [...]

Published
2024

188. U.S. Rejects Putin’s Latest Call for Ukraine Negotiations

Author

Crowley, Michael

Subjects
Diplomatic negotiations in international disputes, Military assistance, American, Pacific settlement of international disputes, News, opinion and commentary
Abstract

Skepticism remains high about the Russian leader’s intentions after he told Tucker Carlson that the war in Ukraine could be settled with a peace deal. The Biden administration dismissed on [...]

Published
2024

189. As Face of Biden’s Israel Policy, Blinken Draws Wrath of Gaza War Protesters

Author

Crowley, Michael

Subjects
United States. Department of State -- International economic relations, Political activists, Israel-Arab conflicts, Reformers, Social reformers, News, opinion and commentary
Abstract

Demonstrators have turned up at the secretary of state’s home and even splashed fake blood on his motorcade. For Secretary of State Antony J. Blinken, the conflict in Gaza has [...]

Published
2024

190. Gaza war protesters direct their ire at Blinken

Author

Crowley, Michael

Subjects
Political activists -- Demonstrations and protests, Reformers -- Demonstrations and protests, Social reformers -- Demonstrations and protests, News, opinion and commentary
Abstract

Protesters angry over Israel’s assault on Gaza have become a regular presence outside the home of Secretary of State Antony J. Blinken in Northern Virginia, with some camping out for [...]

Published
2024

191. State Dept. Plays Down Pause in Aid for Gaza

Author

Crowley, Michael

Subjects
United States. Department of State -- Powers and duties, Israel-Hamas War, 2023-, Humanitarian aid -- Political aspects, Humanitarian workers -- Investigations, Company legal issue, Company financing, General interest, News, opinion and commentary, Hamas -- Military aspects, United Nations. Relief and Works Agency for Palestine Refugees in the Near East -- Officials and employees -- Finance
Abstract

Just $300,000 is on hold after Israeli claims that UNRWA employees joined the Oct. 7 Hamas attacks, U.S. officials say. The State Department downplayed the significance on Tuesday of its [...]

Published
2024

192. Frozen U.S. Spending for U.N. in Gaza Is Minimal, State Dept. Says

Author

Crowley, Michael

Subjects
United States. Department of State, Dollar (United States), News, opinion and commentary
Abstract

Just $300,000 is on hold after Israeli claims that UNRWA employees joined the Oct. 7 Hamas attacks, U.S. officials say. The State Department downplayed the significance on Tuesday of its [...]

Published
2024

193. In Angola, a Former Cold War Rival, Blinken Touts U.S. Investments

Author

Crowley, Michael

Subjects
Solar energy, Investments, News, opinion and commentary
Abstract

Secretary of State Antony J. Blinken finished a four-nation tour of Africa with a visit to Angola, site of major new U.S. investments, which may help counter China’s influence. Secretary [...]

Published
2024

194. Blinken, Ending Africa Tour, Promotes Projects in Angola

Author

Crowley, Michael

Subjects
Solar energy, General interest, News, opinion and commentary
Abstract

Secretary of State Antony J. Blinken finished a four-nation tour of Africa with a visit to Angola, site of major new U.S. investments, which may help counter China's influence. Secretary [...]

Published
2024

195. After a strike on a U.N. building, Blinken says Gaza’s civilians must be protected

Author

Crowley, Michael

Subjects
Israel-Arab conflicts -- Protection and preservation, News, opinion and commentary, United Nations
Abstract

Secretary of State Antony J. Blinken on Thursday emphasized “the imperative of protecting civilians and protecting civilian infrastructure” in Gaza, following a strike on a United Nations facility that U.N. [...]

Published
2024

196. On Africa Trip, Blinken Seeks to Strengthen Ties

Author

Crowley, Michael

Subjects
Secretaries of state -- Powers and duties, Visits of state, General interest, News, opinion and commentary
Abstract

The secretary of state is beginning a tour of four African countries, hoping to promote positive news on the continent. Secretary of State Antony J. Blinken touched down on a [...]

Published
2024

197. On a Remote Island, Blinken Signals U.S. Attention to Africa

Author

Crowley, Michael

Subjects
Mediation, Natural resources -- Cabo Verde -- China -- Sub-Saharan Africa -- Angola, News, opinion and commentary
Abstract

The secretary of state is beginning a tour of four African countries, hoping to promote positive news on the continent. Secretary of State Antony J. Blinken touched down on a [...]

Published
2024

198. U.S. to Return Yemen's Houthis to Terrorism List

Author

Crowley, Michael

Subjects
United States. Department of State, Terrorism, Terrorist organizations, General interest, News, opinion and commentary
Abstract

The move is a partial return to a tougher Trump administration stance toward the Iran-backed group, which has attacked commercial ships in the Red Sea and the Gulf of Aden. [...]

Published
2024

199. U.S. to Return Houthis to Terrorism List

Author

Crowley, Michael

Subjects
Terrorism, Terrorist organizations, News, opinion and commentary
Abstract

The move is a partial return to a tougher Trump administration stance toward the Iran-backed group, which has attacked commercial ships in the Red Sea and the Gulf of Aden. [...]

Published
2024

200. Blinken Makes a Return To the Region as Discord With Israel Keeps Rising

Author

Wong, Edward and Crowley, Michael

Subjects
Assassination of Saleh al-Arouri, 2024 -- Influence, Israel-Hamas War, 2023- -- International aspects, Secretaries of state -- Powers and duties, Prime ministers -- Powers and duties, Visits of state -- Planning, Israel-Arab conflicts -- International aspects, Armistices -- Planning, Humanitarian aid -- Distribution, Civilian casualties -- Forecasts and trends, Company business planning, Market trend/market analysis, Company distribution practices, General interest, News, opinion and commentary
Abstract

Details of a clash between the U.S. secretary of state and Israel's prime minister point to the challenges ahead. Secretary of State Antony J. Blinken is returning to the Middle [...]

Published
2024

Catalog

Books, media, physical & digital resources
See catalog results