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Abstract Background Pressure offloading treatment is critical for healing diabetes‐related foot ulcers (DFU). Yet the 2011 Australian DFU guidelines regarding offloading treatment are outdated. A national expert panel aimed to develop a new Australian guideline on offloading treatment for people with DFU by adapting international guidelines that have been assessed as suitable to adapt to the Australian context. Methods National Health and Medical Research Council procedures were used to adapt suitable International Working Group on the Diabetic Foot (IWGDF) guidelines to the Australian context. We systematically screened, assessed and judged all IWGDF offloading recommendations using best practice ADAPTE and GRADE frameworks to decide which recommendations should be adopted, adapted or excluded in the Australian context. For each recommendation, we re‐evaluated the wording, quality of evidence, strength of recommendation, and provided rationale, justifications and implementation considerations, including for geographically remote and Aboriginal and Torres Strait Islander peoples. This guideline, along with five accompanying Australian DFU guidelines, underwent public consultation, further revision and approval by ten national peak bodies (professional organisations). Results Of the 13 original IWGDF offloading treatment recommendations, we adopted four and adapted nine. The main reasons for adapting the IWGDF recommendations included differences in quality of evidence ratings and clarification of the intervention(s) and control treatment(s) in the recommendations for the Australian context. For Australians with plantar DFU, we recommend a step‐down offloading treatment approach based on their contraindications and tolerance. We strongly recommend non‐removable knee‐high offloading devices as first‐line treatment, removable knee‐high offloading devices as second‐line, removable ankle‐high offloading devices third‐line, and medical grade footwear as last‐line. We recommend considering using felted foam in combination with the chosen offloading device or footwear to further reduce plantar pressure. If offloading device options fail to heal a person with plantar DFU, we recommend considering various surgical offloading procedures. For people with non‐plantar DFU, depending on the type and location of the DFU, we recommend using a removable offloading device, felted foam, toe spacers or orthoses, or medical grade footwear. The six new guidelines and the full protocol can be found at: https://diabetesfeetaustralia.org/new‐guidelines/. Conclusions We have developed a new Australian evidence‐based guideline on offloading treatment for people with DFU that has been endorsed by ten key national peak bodies. Health professionals implementing these offloading recommendations in Australia should produce better DFU healing outcomes for their patients, communities, and country.

A complete ostrich femur from the Late Pleistocene deposits of the Upper Cave at Zhoukoudian (China) was referred by Shaw to Struthio anderssoni in the 1930s, but its present whereabouts are unknown. A good quality plaster cast of the missing specimen has been found in the collections of the Natural History Museum (London). This cast provides interesting information about the morphology of this large ostrich femur, which had previously been only summarily described and not illustrated. Although smaller than the femora of the Early Pleistocene giant ostrich Pachystruthio, the robust femur from Zhoukoudian shows morphological similarities with them, and it is suggested that ‘Struthio’ anderssoni should be placed in the genus Pachystruthio. The importance of old palaeontological casts is emphasized, as well as the need to preserve and curate them properly.

Cyanobacteria are prokaryotic microorganisms with capacity to perform photosynthesis and provide valuable platform to produce high-value bioactive compounds in carbon-neutral pathway. However, due to the relative lack of high throughput genetic manipulation tools for cyanobacteria, their usage for complexity chemicals is lagging behind. The gene editing technology based on the CRISPR-Cas system has the advantage of simplicity and efficiency in recent years, making it a new tool for synthetic biology of cyanobacteria. In this review, we first introduced the CRISPR-Cas system and the types of CRISPR-Cas in cyanobacteria. In addition, we review the development of CRISPR-based systems in cyanobacteria, including the use of CRISPR systems for gene integration, deletion, and transcriptional regulation. Meanwhile, the CRISPR system can couple different functional proteins to achieve point mutations, such as base editing. Finally, we discuss the current limitations of CRISPR technology and its future application prospects in cyanobacteria to make an outlook. Finally, we discussed the current limitations of CRISPR technology and its future application prospects in cyanobacteria.

This study utilized FLUENT dynamic mesh simulation technology to simulate the temperature field distribution characteristics during the continuous casting (CC) process of 5 mm thick Zr 41.2 Ti 13.8 Cu 12.5 Ni 10.0 Be 22.5 (Vit1) bulk metallic glass (BMG), analyzed and discussed the amorphous forming ability of the Vit1 BMG plate prepared through CC. The results indicate that during the CC process, the temperature gradient and cooling rate of Vit1 BMG plate decrease with increasing distance from the cooling copper block surface and prolonged solidification time. Even at the lowest cooling rate, it still remains significantly higher than the critical cooling rate ( R c ) of Vit1 bulk amorphous alloy. The temperature variations recorded by the thermocouple during the alloy melt solidification process are in basic agreement with the simulation data. The experimental test and simulation results show that 5 mm thick Vit1 BMG slab can be prepared theoretically by continuous casting technology. Finally, XRD, DSC and TEM were used to analyze the amorphous formation ability and microstructure of the Vit1 BMG slab., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Genome editing in non-model bacteria is important to understand gene-to-function links that may differ from those of model microorganisms. Although species of the Burkholderia cepacia complex (Bcc) have great biotechnological capacities, the limited genetic tools available to understand and mitigate their pathogenic potential hamper their utilization in industrial applications. To broaden the genetic tools available for Bcc species, we developed RhaCAST, a targeted DNA insertion platform based on a CRISPR-associated transposase driven by a rhamnose-inducible promoter. We demonstrated the utility of the system for targeted insertional mutagenesis in the Bcc strains B. cenocepacia K56-2 and Burkholderia multivorans ATCC17616. We showed that the RhaCAST system can be used for loss- and gain-of-function applications. Importantly, the selection marker could be excised and reused to allow iterative genetic manipulation. The RhaCAST system is faster, easier, and more adaptable than previous insertional mutagenesis tools available for Bcc species and may be used to disrupt pathogenicity elements and insert relevant genetic modules, enabling Bcc biotechnological applications., Importance: Species of the Burkholderia cepacia complex (Bcc) have great biotechnological potential but are also opportunistic pathogens. Genetic manipulation of Bcc species is necessary to understand gene-to-function links. However, limited genetic tools are available to manipulate Bcc, hindering our understanding of their pathogenic traits and their potential in biotechnological applications. We developed a genetic tool based on CRISPR-associated transposase to increase the genetic tools available for Bcc species. The genetic tool we developed in this study can be used for loss and gain of function in Bcc species. The significance of our work is in expanding currently available tools to manipulate Bcc., Competing Interests: The authors declare no conflict of interest.

Purpose: To evaluate the caries prevalence and experience in primary molars in Polish 6- to 7-year-old children using the Caries Assessment Spectrum and Treatment index.Materials and Methods: This cross-sectional study was carried out on a sample of 332 out of 468 invited children (140 6- and 192 7-year-olds). Cluster sampling was used to select the participating schools. Dental status was evaluated according to the criteria dedicated to CAST. The means of the individual CAST scores were assessed. Data were statistically analysed using the Mann-Whitney U-test (p < 0.05). The percentage of children according to the highest CAST score was calculated. The mean values of the dmft index, based on three different caries thresholds--CAST codes 3 to 7 (d3-7t), 4 to 7 (d4-7t) and 5 to 7 (d5-7t)--were calculated.Results: The response rate was 70.9%. One-fifth of the participants showed a healthy, functional dentition (CAST codes 0-2). The highest percentage of children had at least one carious tooth with pulpal involvement (26.2%), followed by children having an obvious dentin caries lesion (20.8%). The means for CAST codes 3, 4 and 5 were 0.61 ± 1.0, 0.19 ± 0.53 and 1.23 ± 1.61, respectively. The means of molars with pulpal involvement was 0.83 ± 1.32 and with dental sepsis 0.07 ± 0.33. Apart from filled teeth, no age-dependent differences were observed. The d3-7mft, d4-7mft and d5-7mft were as follows: 5.18, 4.61 and 4.41, respectively.Conclusion: The surveyed Polish children showed high caries prevalence and severe caries experience in the primary molars. The CAST index was a useful tool for the epidemiological analysis of primary molars. [ABSTRACT FROM AUTHOR]

CRISPR-associated Tn7 transposons (CASTs) co-opt cas genes for RNA-guided transposition. CASTs are exceedingly rare in genomic databases; recent surveys have reported Tn7-like transposons that co-opt Type I-F, I-B, and V-K CRISPR effectors. Here, we expand the diversity of reported CAST systems via a bioinformatic search of metagenomic databases. We discover architectures for all known CASTs, including arrangements of the Cascade effectors, target homing modalities, and minimal V-K systems. We also describe families of CASTs that have co-opted the Type I-C and Type IV CRISPR-Cas systems. Our search for non-Tn7 CASTs identifies putative candidates that include a nuclease dead Cas12. These systems shed light on how CRISPR systems have coevolved with transposases and expand the programmable gene-editing toolkit. [ABSTRACT FROM AUTHOR]

Plastic bronchitis (PB) is a rare and life-threatening complication encountered in several disease states that leads to airway obstruction by branching casts. PB is most often reported in children with cyanotic congenital heart disease where recurrence is common, and mortality is high. There is limited data on optimal management strategies or recurrence of non-structural heart disease–related PB in children. We describe the clinical features, management, and outcomes in our cohort of children with non-structural heart disease-related PB. Among the 12 identified patients, asthma was the most common (67%) diagnosis. Ventilatory requirements ranged from room air to one patient who required extracorporeal membrane oxygenation (ECMO). Most patients (92%) required bronchoscopy, and cryotherapy was successfully utilized in two patients to relieve refractory obstructive airway casts. All patients received chest physiotherapy, and 11 patients were treated with two or more medications. There was one mortality despite ECMO, and one-third had recurrent PB, all of whom had asthma. Conclusion: Asthma is a risk factor for recurrent PB. Bronchoscopic interventions including cryotherapy are safe and effective treatment options in patients with refractory PB. What is Known: • Plastic bronchitis is a rare but life-threatening cause of airway obstruction caused by branching casts that are generally reported in patients with congenital heart disease. What is New: • In children without structural heart disease, asthma is a risk factor for recurrent plastic bronchitis. Cryotherapy via bronchoscopy is a safe and effective intervention in patients with refractory plastic bronchitis. [ABSTRACT FROM AUTHOR]

Background: In the conservative management of distal radial fractures (DRFs), the optimal dorsi-volar angulation of the wrist at cast immobilization and proper cast molding to minimize the risk of redisplacement are unclear. This study identified the predictors of fracture redisplacement during cast immobilization for adult DRFs. Methods: We examined for dorsi-volar angulation, gap index, volar tilt (VT), radial inclination (RI), and radial length (RL) in lateral or posteroanterior radiographs of 90 DRFs. We investigated possible predisposition factors for redisplacement including patient age, sex, extra- or intra-articular fracture, metaphyseal comminution, original displacements, dorsi-volar angulation of the wrist at cast immobilization, restoration of the volar cortex at cast immobilization, and gap index of the cast. Results: Neither dorsi-volar angulation nor gap index had significant association with an unacceptable alignment nor decrease of VT, RI, and RL. In multivariate analysis, patient age, original displacement, and intra-articular fracture were the significant predictors of an unacceptable alignment or decrease of VT and RI. Conclusions: Our findings indicate dorsi-volar angulation and cast molding quality have no clinical effect on preventing fracture redisplacement. The predictive factors of the displacement were patient age, original displacement, and intra-articular fracture. [ABSTRACT FROM AUTHOR]

Among the applications of aluminum-based alloys is that in the field of hydraulic installations and equipment. Some components of their structure, such as the rotors of the cooling pumps of motor vehicles, or the blades of propellers from the engines of fishing boats, or pleasure boats, and even the radiators of motor vehicles are subject to corrosion by the cavity created during operation. Among the aluminum alloys used in the manufacture of such components is the alloy 5083. These parts can be made by casting (rotors / pump housings and steam propeller) or laminated semi-finished products, such as blades for rotors / propellers with adjustable vanes. Cavitation operation inevitably leads to damage by erosion caused by cyclic stresses of micro-jets and shock waves generated by the implosion of cavitation bubbles. As a result, the paper presents the results regarding the degradation by vibration cavitation of alloy 5083, in the form of cast and rolled semi-finished product. The curves and parameters specific to the resistance of the alloy to cavity erosion, as well as the images of eroded surfaces, show, surprisingly, that the cast semi-finished product has a higher resistance than the laminated one, due to the mechanical properties and the type of microstructure. [ABSTRACT FROM AUTHOR]

Introduction: Data from clinical trials suggest that CT-confirmed nondisplaced scaphoid waist fractures heal with less than the conventional 8-12 weeks of immobilization. Barriers to adopting shorter immobilization times in clinical practice may include a strong influence of fracture tenderness and radiographic appearance on decision-making. This study aimed to investigate (1) the degree to which surgeons use fracture tenderness and radiographic appearance of union, among other factors, to decide whether or not to recommend additional cast immobilization after 8 or 12 weeks of immobilization; (2) identify surgeon factors associated with the decision to continue cast immobilization after 8 or 12 weeks.Materials and Methods: In a survey-based study, 218 surgeons reviewed 16 patient scenarios of CT-confirmed nondisplaced waist fractures treated with cast immobilization for 8 or 12 weeks and recommended for or against additional cast immobilization. Clinical variables included patient sex, age, a description of radiographic fracture consolidation, fracture tenderness and duration of cast immobilization completed (8 versus 12 weeks). To assess the impact of clinical factors on recommendation to continue immobilization we calculated posterior probabilities and determined variable importance using a random forest algorithm. Multilevel logistic mixed regression analysis was used to identify surgeon characteristics associated with recommendation for additional cast immobilization.Results: Unclear fracture healing on radiographs, fracture tenderness and 8 (versus 12) weeks of completed cast immobilization were the most important factors influencing surgeons' decision to recommend continued cast immobilization. Women surgeons (OR 2.96; 95% CI 1.28-6.81, p  =  0.011), surgeons not specialized in orthopedic trauma, hand and wrist or shoulder and elbow surgery (categorized as 'other') (OR 2.64; 95% CI 1.31-5.33, p  =  0.007) and surgeons practicing in the United States (OR 6.53, 95% CI 2.18-19.52, p  =  0.01 versus Europe) were more likely to recommend continued immobilization.Conclusion: Adoption of shorter immobilization times for CT-confirmed nondisplaced scaphoid waist fractures may be hindered by surgeon attention to fracture tenderness and radiographic appearance. [ABSTRACT FROM AUTHOR]

There is approximately a 2% risk of clinically significant VTE following temporary lower limb immobilisation after injury with an ankle immobilising plaster cast or boot. There is evidence that thromboprophylaxis for lower limb immobilised patients reduces the risk of VTE by approximately 50% but there is no international consensus as to which patients should receive thromboprophylaxis. The Plymouth VTE Risk Score was developed to identify patients at particular risk of VTE, in order to offer chemical prophylaxis to reduce their VTE risk. The score showed high completion rates, reliability and consistency. Using the Plymouth VTE Risk Score Version 3 (2014), we found an incidence of clinical VTE of 0.36% with 37.6% of patients being advised to have thromboprophylaxis. This is a lower VTE incidence than in most other studies of this patient group, which is of the order of 2 to 3%. The optimal RAM to use in clinical practice is yet to be defined, further clinical research is needed to accurately stratify patient risk and to define optimal risk treatment levels. We suggest research should focus on comparative clinical studies of risk assessment models. [ABSTRACT FROM AUTHOR]

Introduction: The objective of this cohort study was to investigate whether the revised post-Cardiac Arrest Syndrome for Therapeutic hypothermia score (rCAST), which we previously developed as a prognostic score for adult patients with post-cardiac arrest syndrome (PCAS), is also applicable to pediatric patients.Methods: Pediatric PCAS patients were included from an out-of-hospital cardiac arrest (OHCA) registry of the Japanese Association for Acute Medicine (JAAM). We validated the predictive accuracy of the rCAST for the neurological outcomes at 30 and 90 days. We also evaluated the probability of a good neurological outcome in each of the three specified severity categories based on the rCAST (low severity: ≤5.5; moderate severity: 6.0-14.0; high severity: ≥14.5).Results: Among the 737 pediatric patients with OHCA, the data of 179 pediatric PCAS patients in whom return of spontaneous circulation was achieved were analyzed. The areas under the curve (AUC) of the rCAST for predicting the neurological outcomes at 30 days and 90 days were 0.95 (95% CI: 0.90-0.99) and 0.96 (0.91-1.00), respectively. The proportions of patients with a good neurological outcome at 30 days were 100% (12/12) in the low severity group, 36.1% (13/36) in the moderate severity group, and 2.3% (3/131) in the high severity group.Conclusions: The AUC of the rCAST for pediatric PCAS patients was found to be greater than 0.9 in the external validation, which corresponds to excellent predictive accuracy. There was no patient with good neurological outcome among the patients with more than 17.0 points (extremely high severity group). [ABSTRACT FROM AUTHOR]

We report on our experience of using a short, moulded metacarpal cast over a 4-year period in the non-surgical management of metacarpal shaft fractures. Between 6 April 2015 and 6 April 2019, 117 patients were treated using this method. The majority were male, and the mean age was 24.6 years. The most common mechanism of injury was a punch injury (73 patients, 62%). Fifteen patients (13%) were treated for multiple metacarpal fractures. Immediately after cast removal, 68% had a full range of finger movement and 68% were pain free. Few required formal hand therapy. Fifteen patients experienced minor complications, primarily soft tissue irritation from the cast and non-limiting extensor lag. The moulded short metacarpal cast is an effective non-surgical treatment for angulated extra-articular metacarpal fractures of the diaphysis and diametaphyseal junction. Level of evidence: IV [ABSTRACT FROM AUTHOR]

Advancements in dental materials and techniques enabled dentists to provide complex prosthetic treatments to patients. Dental articulator is an indispensable tool to enhance the quality of the delivered work and increase patient satisfaction1. It is the purpose of this paper to present an overview of dental articulators, their different types, indications, advantages and limitations. A practical guide for selecting the right articulator for individual clinical situations is provided. [ABSTRACT FROM AUTHOR]

Origin and Evolution of Caste system in Tamil Nadu is an attempt to trace out the Meaning and Definition and Changes in the Caste system through the ages. Generally India is a land of several religions, races and inequalities. Society in the Indian sub-continent has been segmented into several religions and caste groups. Majority of the people belongs to the Hindu religion in India. The Hindu society is a composite and comprises of several castes and sub-castes. These castes are not horizontal divisions of the society. Castes occupy various levels in the ladder of Hindu social hierarchy. A man had no individual identity in the past. Only caste rights and caste privileges were recognized and maintained through the ages. These castes were considered as prime part of units of the society people enjoyed varying status and rights. The social inequality was believed as divinely ordained. However, this system had to have faced lot of challenges from time to time. Whenever the challenges happened to appear, the Hindu society and the system followed so far underwent some modifications to suit the need of the society. Of course, the hierarchical system of the society was never given up by the caste Hindus. Several social reformers, leaders and administrators have tried to eradicate the social inequalities but it has been continuing with slight modifications and these efforts have been taken up ever since the period of Gautama Buddha. Under the British, several measures were introduced by the Government to bring about a change is the social condition of Tamil Nadu. However it could not be successful because people had difference of opinion among themselves. [ABSTRACT FROM AUTHOR]

This study focused on the role of Cu in the microstructure characteristics and tensile properties of novel L12-strengthened multicomponent high-entropy alloys (HEAs). A series of as-cast (Al7.5Co21.9Cr10.9Ti5.0Fe21.9Ni32.8)100-xCux (x = 0.5, 2.5, 5.0) high-entropy alloys (HEAs) were prepared. The microstructures and mechanical properties of HEAs were investigated using X-ray diffraction, a scanning electron microscope, a transmission electron microscope, and atom probe tomography. The XRD patterns of HEAs confirmed that all HEAs consisted of the FCC phase and the L12 phase. As Cu content increased, the dendritic was gradually coarsened. The spherical L12 size decreased, and number density increased in the interdendritic regions (ID). The L12 mainly contained Ni, Ti, Al, and Cu. The acicular L12 size increased and was continuously distributed in the dendritic regions (DR) as the Cu content increased gradually. The ultimate strength and elongation decreased from 1,002 MPa, 20.0% to 906 MPa, 13.1%, respectively. The segregation rates of Ti, Cu, and Al increased in the DR and ID. The L12 nano-precipitates in the DR become denser and finer, while the L12 islets in the ID region increase and elongate. Large lattice distortion caused by Cu addition weakens the strength of the L12-FCC phase boundary, leading to the premature fracture of the three HEAs, which were the main reasons for the decreases in strength and ductility as Cu content increased.

Tenderness is one of the main characteristics of meat because it determines its price and acceptability. This is the first bibliometric study on the trend of research on the role of genes in meat tenderness. A total of 175 original and English-language articles published up to 2021 were retrieved from Scopus. The bibliometric analysis was carried out with VOSviewer (version 1.6.18, Eck and Waltman, Leiden, Netherlands) and complemented with the Analyze search results service from Scopus. Erroneous and duplicate data were eliminated, and incomplete information was added to standardize the results. Scientific production was evaluated by means of quantity, quality and structure indicators. As a first glance, 8.816% of authors have published more than 50% of papers mainly related to genes encoding the calpain (CAPN)-calpastatin (CAST) system and single nucleotide polymorphisms (SNPs). Among other findings, a strong link was found between the contribution of the main countries (led by the United States with) and their institutions (led by the USDA Agricultural Research Service with) to their gross domestic product. Most studies on the topic are published in the Journal of Animal Science, and other journals with high impact according to the number of citations and different metrics. Finally, when evaluating the most cited articles, the occurrence and association of the main keywords, it was confirmed that research is focused on the role of CAPN and CAST genes and of SNPs in beef tenderness. The change in science was emphasized; although marker-assisted selection is still used, genes have an infinitesimal effect on complex traits. Therefore, since about 2010, new research groups adopted genomic selection to evaluate dense panels of SNPs and better explain genetic variation in meat tenderness.

Context: Ankle immobilization is often used after ankle injury. Objective: To determine the influence of 1 week's unilateral ankle immobilization on plantar-flexor strength, balance, and walking gait in asymptomatic volunteers. Design: Repeated-measures laboratory study. Setting: University laboratory. Participants: 6 physically active male participants with no recent history of lower-limb injury. Interventions: Participants completed a 1-wk period of ankle immobilization achieved through wearing a below-knee ankle cast. Before the cast was applied, as well as immediately, 24 h, and 48 h after cast removal, their plantar-flexor strength was assessed isokinetically, and they completed a single-leg balance task as a measure of proprioceptive function. An analysis of their walking gait was also completed Main Outcome Measures: Peak plantar-flexor torque and balance were used to determine any effect on muscle strength and proprioception after cast removal. Ranges of motion (3D) of the ankle, knee, and hip, as well as walking speed, were used to assess any influence on walking gait. Results: After cast removal, plantar-flexor strength was reduced for the majority of participants (P = .063, CI = -33.98 to 1.31) and balance performance was reduced in the immobilized limb (P < .05, CI = 0.84-5.16). Both strength and balance were not significantly different from baseline levels by 48 h. Walking speed was not significantly different immediately after cast removal but increased progressively above baseline walking speed over the following 48 h. Joint ranges of motion were not significantly different at any time point. Conclusions: The reduction in strength and balance after such a short period of immobilization suggested compromised central and peripheral neural mechanisms. This suggestion appeared consistent with the delayed increase in walking speed that could occur as a result of the excitability of the neural pathways increasing toward baseline levels. [ABSTRACT FROM AUTHOR]

Background: Immobilisation following surgical treatment of distal radial fractures (DRF) is traditionally performed with a dorsal cast splint. There is an interest in changing the rigid cast to a removable brace. This can reduce the risk for cast-corrections, complications and improve recovery of function. The aim of the study was to compare quality of recovery (QoR) between brace and traditional cast for immobilisation during the first postoperative week. Methods: 60 patients with American Society of Anesthesiologists (ASA) physical status 1–3, scheduled for surgical treatment of DRF under a supraclavicular block (SCB) in a day-surgery setting were randomised into two groups of immobilisation post-surgery; brace (n=30) versus traditional cast (n=30). Study objectives were: differences in self-assessed QoR using the QoR-15 questionnaire, postoperative oral oxycodone consumption, perioperative time events and unplanned healthcare contacts one week postoperatively. Results: 54 patients, 46 females/eight males were included in the analysis; 27 with brace and 27 with traditional cast. QoR-15 sum median scores improved significantly from baseline/preoperative to day 7 (brace p=0.001, cast p=0.001) with no differences between the two groups. The only difference found was that patients in the brace group had significantly worse pain score 24-hours post-surgery (p=0.022). No significant differences were seen in sum median oxycodone consumption the first three postoperative days. No differences were found in perioperative events or unplanned healthcare contacts. Conclusions: Brace appears to be a feasible option to traditional cast for immobilisation following surgical treatment of DRF. The early QoR was similar in both groups apart from more pain in the brace group the first 24 postoperative hours.

Calpain-mediated proteolysis has been proposed to modulate the pathogenesis of spinocerebellar ataxia type 3, also known as Machado-Joseph disease (SCA3/MJD), a disorder due to a CAG repeat expansion (CAGexp) at ATXN3. We aimed to investigate if single-nucleotide polymorphisms (SNPs) at calpain gene CAPN2 and at calpastatin gene CAST modulate the age at onset (AO) and disease progression in SCA3/MJD. A total of 287 SCA3/MJD symptomatic subjects (151 families) were included. AO was analyzed and controlled by the CAG repeat length of expanded allele and family. Candidate polymorphisms were chosen based on the literature and on a priori criteria. The CAG repeat length and SNPs were genotyped according to standard methods. AO of carriers of AA and AG + GGrs1559085 genotypes in CAST and with the median value of 75 repeats at the expanded allele were 34.23 (33.07–35.38) and 36.42 years (34.50–38.34), respectively (p = 0.049, mixed model treating the expanded CAG repeat size as fixed effect and family as random effect). Carriers of haplotype Crs27852/Grs1559085 had mean AO of 37.23 (12.76) and 33.42 years (12.20) (p = 0.047, Student's t test). Our data suggest an association between allele Grs1559085 and haplotype Crs27852/Grs1559085 at CAST and variations in the AO of SCA3/MJD subjects, independent from the effects of the CAGexp and family. The present results support the potential role of calpain cleavage pathway over modulation of SCA3/MJD phenotype. [ABSTRACT FROM AUTHOR]

Introduction: Refracture of the tibia after union is a challenging problem for the patients and the surgeons. The purpose of the current study is to present our experience in conservative management of such patients with refracture of infected ununited tibia after successful treatment by Ilizarov external fixator and bone transport.Material and Methods: We reviewed the files of 812 patients with infected ununited tibia who were treated by debridement, corticotomy, and bone transport using Ilizarov methods in our institute between 1997 and 2017. Inclusion criteria were patients with refracture after union and removal of the Ilizarov apparatus. Twenty-two patients with 23 refracture tibia were included in the study. All the 23 tibias were treated conservatively by above knee cast that was converted to Sarmiento below knee cast after early callus formation, except in the case of upper tibial fracture that continued in above knee cast till union. Afterwards, a protective splint was used for additional two months.Results: There were 19 males (86.4%) and three females (13.6%), the mean age of the patients was 38.39 years, the mean time of Ilizarov external fixator application was 10.86 months (range, 6-17), and the mean time of refracture after fixator removal was 2.33 months. Union was achieved in 19 tibias (82.6%), with a mean time of 7.2 months (range, 4-12). Complications included five cases of skin irritation that was treated by large windows in the cast and changing the casts more frequently, three cases developed DVT (deep venous thrombosis), and axial deviation occured in four tibias (17.3%).Conclusion: Conservative treatment of refractured tibia after removal of Ilizarov external fixator following treatment of infected non-union tibia by above knee cast is effective in achieving union. However, complications as skin irritation, DVT (deep venous thrombosis), and axial deviation can be anticipated. [ABSTRACT FROM AUTHOR]

This study investigated the impact of the equal channel angular pressing (ECAP) combined with heat treatments on the microstructure and mechanical properties of AlSi10Mg alloys fabricated via selective laser melting (SLM) and gravity casting. Special attention was directed towards determining the effect of post-fabrication heat treatments on the microstructural evolution of AlSi10Mg alloy fabricated using two different routes. Three initial alloy conditions were considered prior to ECAP deformation: (1) as-cast in solution treated (T4) condition, (2) SLM in T4 condition, (3) SLM subjected to low-temperature annealing. Light microscopy, transmission electron microscopy, X-ray diffraction line broadening analysis, and electron backscattered diffraction analysis were used to characterize the microstructures before and after ECAP. The results indicated that SLM followed by low-temperature annealing led to superior mechanical properties, relative to the two other conditions. Microscopic analyses revealed that the partial-cellular structure contributed to strong work hardening. This behavior enhanced the material's strength because of the enhanced accumulation of geometrically necessary dislocations during ECAP deformation. [ABSTRACT FROM AUTHOR]

The decision to continue immobilization of a nondisplaced scaphoid waist fracture is often based on radiographic appearance (despite evidence that radiographs are unreliable and inaccurate for diagnosing scaphoid union 6–12 weeks after fracture) and fracture tenderness (even though it is influenced by cognitive biases on pain). This may result in unhelpful additional immobilization. We studied nondisplaced scaphoid waist fractures to determine the factors associated with (1) the surgeon's decision to continue cast or splint immobilization at the first visit when cast removal was being considered; (2) greater pain on examination; and (3) the surgeon's concern about radiographic consolidation. We prospectively included 46 patients with a nondisplaced scaphoid waist fracture treated nonoperatively. At the first visit when cast removal was considered – after an average of 6 weeks of immobilization – patients rated pain during 4 examination maneuvers. The treating surgeon assessed union on radiographs and decided whether to continue or discontinue immobilization. Patients completed measures of the following: (1) the degree to which pain limits activities (Patient-Reported Outcome Measure Interactive System [PROMIS] Pain Interference Computer Adaptive Test [CAT], Pain Self-Efficacy Questionnaire-2); (2) symptoms of depression (PROMIS Depression CAT); and (3) upper extremity function (PROMIS Upper Extremity Function CAT). We used multivariable regression analysis to investigate the factors associated with each outcome. Perceived inadequate radiographic healing and greater symptoms of depression were independently associated with continued immobilization. Pain during the examination was not associated with continued immobilization. Patient age was associated with pain on examination. Shorter immobilization duration was the only factor associated with the surgeon's perception of inadequate radiographic consolidation. Inadequate radiographic healing and greater symptoms of depression are associated with a surgeon's decision to continue cast or splint immobilization of a nondisplaced scaphoid waist fracture. Overreliance on radiographs and inadequate accounting for psychological distress may hinder the adoption of shorter immobilization times for nondisplaced waist fractures. [ABSTRACT FROM AUTHOR]

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The chilled cast iron tappet comprises three gradient microstructures, including chill, mottle, and gray iron. In this work, the effect of Tellurium (Te) on the chilled iron tappet's microstructure and properties is studied. The addition of Te to gray cast alloy enhances its mechanical and thermal properties, which can be used in new designing of materials for heavy automotive applications. The gray cast iron alloy with 0.01 to 0.05 wt.% of Te was prepared in order to examine the effect of Te wt.% on the chill depth, microstructure, wear, and hardness. The scanning electron micrographs are used to distinguish and analyze the chill zone, mottle, and gray zone in the tappet, and the hardness was studied by Vickers hardness test, and wear behavior by a pin on disc method was measured. The present study showed that the addition of Te strongly influenced the formation of chill and efficiently enhanced the tappet's mechanical properties along with the favorable effect to eliminate the mottled zone and separate the chill zone and gray zone with a clear boundary. [ABSTRACT FROM AUTHOR]

This article investigates the 1550s culture of honour that focused primarily on the expressiveness of the (male) individual, rather than on content, which means that the code of honour was not a system of strictly defined normative rules; on the contrary, rules were formed based on the model of behaviour of a certain group. The purpose was thus to describe and popularise the pattern of honourable conduct through literary culture or published texts. The treatises on duelling served not only as manuals on how to approach or, better, how to avoid physical combat without losing honour, but also as reading for the courtiers to draw on when arguing, discussing, and citing (printed) opinions on duelling judgments and cases. The discourse and representation of duelling were forms of spectacle intended for life at court, and understood within that same context. Not surprisingly, the most eminent authors on duelling were courtiers. The study focuses on two examples of treatise: the “practical,” penned by the Bologna fencing master Achille Marozzo, and the “theoretical,” authored by the Koper man of letters Girolamo Muzio. [ABSTRACT FROM AUTHOR]

• The NiCoCr alloys were prepared by mechanical vibration solidification. • The microstructure of as-cast NiCoCr alloys is significantly refined. • The yield stress increases at room temperature without loss of elongation. The NiCoCr multi principal element alloy (MPEA) show excellent ductility at cryogenic temperatures and corrosion-resistance properties under the condition that it prepared by thermal–mechanical processing. However, the coarse as-cast grains negatively impact mechanical properties significantly and grain refinement is necessary for forming complex castings. Most grain refining methods need extra alloying elements, which may result in loss of ductility by brittle second phases. In this work, a significant microstructure refinement of the as-cast NiCoCr MPEA was achieved by pollution-free vacuum mechanical vibration, with the average grain size refined from the initial value of 620 μm to 140 μm. As a result, the room-temperature yield strength was increased by 54 % (from 255 MPa to 392 MPa) without loss of elongation compared to the as-cast condition without vibration. [ABSTRACT FROM AUTHOR]

• A newly designed ceramic shot sleeve is proposed in this work. • The comparison of porosity and externally solidified crystals using steel shot sleeve and ceramic shot sleeve is studied. • Both porosity and ESCs achieve an effective reduction after applying the ceramic shot sleeve. The effective methods to regulating porosity and externally solidified crystals (ESCs) have been remained as a challenging task for high pressure die casting (HPDC) automobile parts. In this work, a newly designed ceramic shot sleeve is proposed for reducing porosity and optimizing ESCs. As a result, both porosity and ESCs achieve an effective reduction after applying the ceramic shot sleeve. This study provides insights into regulating porosity and coarse ESCs of die castings and it is expected to expand its application in industrial manufacture. [ABSTRACT FROM AUTHOR]

A recently uncovered assemblage of 13 coins, some of significant dating value, but all loose finds from fieldwork conducted by the Polish–Egyptian Conservation Mission, is discussed in the context of earlier coin finds recorded by the two Polish projects involved in the archaeological excavation and conservation of the Marina el-Alamein site on the Mediterranean coast of Egypt. The focus is foremost on predominantly Roman provincial coins originating from the Alexandrian mint. One of these bears a mark indicative of its use as a pendant. Hadrian bronzes, most numerous in this group, along with coins of Trajan and Antoninus Pius corroborate a peak in the development of the town in the 2nd century AD, while late Roman imperial specimens are direct evidence for its continued functioning in the late antique period.

Abstract Background Bone fractures are one of the most common injuries in the USA. Fiberglass tape is a commonly used casting material, and many medical professionals apply adjuvants including liquid hand soap, foam sanitizers, and ultrasound gel in the hopes of improving outcomes relating to ease of molding and eventual strength, lamination, and smoothness of cast material. However, the efficacy of these agents to improve fiberglass cast mechanics has not been scientifically evaluated. The purpose of this study was to assess the mechanical effects of commonly used adjuvants on fiberglass cast materials. Methods Studies compared regularly shaped samples of water-activated, untreated fiberglass tape (Ossur Techform Premium) to water-activated fiberglass tape treated with one of three commonly used adjuvants (liquid soap, foam hand sanitizer, or ultrasound gel) during lamination. Material stiffness, yield stress, and ultimate load were measured by 3-point bending. Results These studies demonstrated that that liquid soap and ultrasound gel did not affect fiberglass tape mechanical properties, but alcohol-based foam sanitizer significantly reduced stiffness (− 32.8%), yield stress (− 33.6%), and ultimate load (− 31.0%) of the cast material as compared to the control group. Regression slopes were not significantly different between groups, suggesting that no adjuvants improved material curing time. Conclusions These data suggest that the application of adjuvants is not beneficial and potentially harmful to fiberglass cast behavior. Despite the widespread practice of adjuvant application by medical professionals during casting, results from the current study suggest that use of these agents for structural enhancement of fiberglass casts is not beneficial and should largely be discouraged.

For industrial in-series castings, the presence of casting defects, like porosity, geometrically complex intermetallic precipitates and the occurrence of eutectic silicon of varying morphologies, is well known but hard to prevent due to technical and economic limitations. To improve the performance and safety of cast aluminum products, the present work deals with the correlation between the cooling-rate-dependent secondary dendrite arm spacing (SDAS), the high- and very-high-cycle-fatigue (VHCF) behavior, the crack propagation mechanisms under pure bending with a focus on the crack propagation paths for near-threshold stress intensity factor ranges ?KI using two conventional automotive cast aluminum alloys AlSi8Cu3 (engine blocks) and AlSi7Cu0.5Mg (cylinder heads). Furthermore, the role of a variation in porosity on the crack initiation process is discussed. Specimens were extracted from in-series castings choosing positions with a maximal difference in cooling rate and SDAS, respectively. It is shown that porosity is highly influencing the crack initiation mechanism and that the SDAS has a strong influence on both, crack propagation rate and crack propagation paths. Hence, analogies between the SDAS and the grain size as influence factor according to the Hall-Petch relationship were identified.

This study aims at assessing the effect of solution heat treatment (at a temperature just below the eutectic temperature) followed by various cooling rates on the microstructure and mechanical properties of additively manufactured AlSi10Mg and the cast counterpart. The mechanical properties were evaluated using a depth-sensing nanoindentation platform. The cast and additively manufactured parts were solutionized at 540 ​°C for 2 ​h followed by water quenching, air cooling, and furnace cooling. Results show extensive microstructural changes (e.g. size and morphology of eutectic-silicon phase) and evolutions in the mechanical properties of the heat-treated materials relative to the as-printed and as-cast ones. Besides, the microstructure and micromechanical properties of the materials broadly alter the cast and additive manufacturing conditions. Depending on the cooling condition, the mentioned cooling cycles directly affect the morphology of eutectic-silicon in both cast and additive manufactured materials starting with silicon fragmentation, then followed by silicon spheroidization, and silicon coarsening. The microstructural evolution affects the local micromechanical properties of the studied materials. The results of this study provide insights into the control of microstructure and hence mechanical properties of AlSi10Mg alloy by addressing suitable heat treatment cycles. This study, for the first time, assesses and compares the effect of various post-fabrication cooling rates in the cast and additive manufacturing conditions in an AlSi10Mg alloy.

Background: Postoperative management regimes vary following open reduction and internal fixation (ORIF) of unstable ankle fractures. There is an evolving understanding that extended periods of immobilisation and weight-bearing limitation may lead to poorer clinical outcomes. Traditional non-weight-bearing cast immobilisation may prevent loss of fixation, and this practice continues in many centres. The purpose of this trial is to investigate the safety and efficacy of immediate weight-bearing (IWB) and range of motion (ROM) exercise regimes following ORIF of unstable ankle fractures with a particular focus on functional outcomes and complication rates. Methods: A pragmatic randomised controlled multicentre trial, comparing IWB in a walking boot and ROM within 24 h versus non-weight-bearing (NWB) and immobilisation in a cast for 6 weeks, following ORIF of all types of unstable adult ankle fractures (lateral malleolar, bimalleolar, trimalleolar with or without syndesmotic injury) is proposed. All patients presenting to three trauma units will be included. The exclusion criteria will be skeletal immaturity and tibial plafond fractures. The three institutional review boards have granted ethical approval. The primary outcome measure will be the functional Olerud-Molander Ankle Score (OMAS). Secondary outcomes include wound infection (deep and superficial), displacement of osteosynthesis, the full arc of ankle motion (plantar flexion and dorsal flection), RAND-36 Item Short Form Survey (SF-36) scoring, time to return to work and postoperative hospital length of stay. The trial will be reported in accordance with the CONSORT statement for reporting a pragmatic trial, and this protocol will follow the SPIRIT guidance. Discussion: Traditional management of operatively treated ankle fractures includes an extended period of non-weight-bearing. There is emerging evidence that earlier weight-bearing may have equivocal outcomes and favourable patient satisfaction but higher wound-related complications. These studies often preclude more complicated fracture patterns or patient-related factors. To our knowledge, immediate weight-bearing (IWB) following ORIF of all types of unstable ankle fractures has not been investigated in a controlled prospective manner in recent decades. This pragmatic randomised-controlled multicentre trial will investigate immediate weight-bearing following ORIF of all ankle fracture patterns in the usual care condition. It is hoped that these results will contribute to the modern management of ankle fractures. Trial registration: ISRCTN Registry ISRCTN76410775. Retrospectively registered on 30 June 2019. [ABSTRACT FROM AUTHOR]

Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative (copy-and-paste) pathways, depending on how the mobile element is excised from its donor source. In the well-characterized E. coli transposon Tn7, a heteromeric TnsA-TnsB transposase directs cut-and-paste transposition by cleaving both strands at each transposon end during the excision step. Whether a similar pathway is involved for RNA-guided transposons, in which CRISPR-Cas systems confer DNA target specificity, has not been determined. Here, we apply long-read, population-based whole-genome sequencing (WGS) to unambiguously resolve transposition products for two evolutionarily distinct transposon types that employ either Cascade or Cas12k for RNA-guided DNA integration. Our results show that RNA-guided transposon systems lacking functional TnsA primarily undergo copy-and-paste transposition, generating cointegrate products that comprise duplicated transposon copies and genomic insertion of the vector backbone. Finally, we report natural and engineered transposon variants encoding a TnsAB fusion protein, revealing a novel strategy for achieving RNA-guided transposition with fewer molecular components. [ABSTRACT FROM AUTHOR]

The calpain system is required by many important physiological processes, including the cell cycle, cytoskeleton remodelling, cellular proliferation, migration, cancer cell invasion, metastasis, survival, autophagy, apoptosis and signalling, as well as the pathogenesis of a wide range of disorders, in which it may function to promote tumorigenesis. Calpains are intracellular conserved calcium‐activated neutral cysteine proteinases that are involved in mediating cancer progression via catalysing and regulating the proteolysis of their specific substrates, which are important signalling molecules during cancer progression. μ‐calpain, m‐calpain, and their specific inhibitor calpastatin are the three molecules originally identified as comprising the calpain system and they contain several crucial domains, specific motifs, and functional sites. A large amount of data supports the roles of the calpain–calpastatin system in cancer progression via regulation of cellular adhesion, proliferation, invasion, metastasis, and cellular survival and death, as well as inflammation and angiogenesis during tumorigenesis, implying that the inhibition of calpain activity may be a potential anti‐cancer intervention strategy targeting cancer cell survival, invasion and chemotherapy resistance. [ABSTRACT FROM AUTHOR]

Background: Fat deposition affected meat quality and the suitable amount of intramuscular fat (IMF) can significantly improve the taste of meat, palatability, juicy, tenderness and flavor, thereby enhancing the meat quality in animals. Although, calpastatin (CAST) and peroxisome proliferator-activated receptor-γ (PPARγ) could regulate meat quality by altering meat tenderness or IMF content in animals, reports concerning the relationship between CAST, PPARγ expression and pork quality is unclear. Methods: A total of twenty 28-day-old purebred weaned Jinhua and Landrace piglets (10 piglets per breed) were divided into two groups according to breed for the feeding trials lasting for 120 days. At the 148 days of age, 3 pigs of each breed were selected to slaughter. Total RNA was extracted from longissimus dorsi and biceps femoris muscle to investigate the differential expression of PPARγ and CAST in muscle tissues of different breed pigs by RT-PCR methods, as well as their relationship with IMF and carcass lean content. Result: These results suggested that PPARγ is an important effector for regulating fat deposition in pigs, which was correlated with IMF and carcass lean content of pigs. This data would provide a scientific basis for the regulation of pork quality. [ABSTRACT FROM AUTHOR]

Background: There is no consensus concerning the optimal casting technique for displaced distal radius fractures (DRFs) following closed reduction. This study evaluates whether a splint or a circumferential cast is most optimal to prevent fracture redisplacement in adult patients with a reduced DRF. Additionally, the cost-effectiveness of both cast types will be calculated.Methods/design: This multicenter cluster randomized controlled trial will compare initial immobilization with a circumferential below-elbow cast versus a below-elbow plaster splint in reduced DRFs. Randomization will take place on hospital-level (cluster, n = 10) with a cross-over point halfway the inclusion of the needed number of patients per hospital. Inclusion criteria comprise adult patients (≥ 18 years) with a primary displaced DRF which is treated conservatively after closed reduction. Multiple trauma patients (Injury Severity Score ≥ 16), concomitant ulnar fractures (except styloid process fractures) and patients with concomitant injury on the ipsilateral arm or inability to complete study forms will be excluded. Primary study outcome is fracture redisplacement of the initial reduced DRF. Secondary outcomes are patient-reported outcomes assessed with the Disability Arm Shoulder Hand score (DASH) and Patient-Rated Wrist Evaluation score (PRWE), comfort of the cast, quality of life assessed with the EQ-5D-5L questionnaire, analgesics use, cost-effectiveness and (serious) adverse events occurence. In total, 560 patients will be included and followed for 1 year. The estimated time required for inclusion will be 18 months.Discussion: The CAST study will provide evidence whether the type of cast immobilization is of influence on fracture redisplacement in distal radius fractures. Extensive follow-up during one year concerning radiographic, functional and patient reported outcomes will give a broad view on DRF recovery.Trial Registration: Registered in the Dutch Trial Registry on January 14th 2020. Registration number: NL8311 . [ABSTRACT FROM AUTHOR]

We describe an 11‐year‐old girl with PLACK Syndrome (peeling skin, leukonychia, acral punctate keratosis, cheilitis, and knuckle pads), who was found to have a novel homozygous variant in CAST, the pathogenicity of which was confirmed using blood‐derived RNA. There is no established treatment for PLACK syndrome. However, we demonstrate for the first time that this condition is associated with low levels of vitamin A and essential fatty acids, which prompted us to consider a potential treatment strategy. Indeed, we initiated this patient on intravenous lipid infusion (Vitalipid®; an emulsion of fat‐soluble vitamins and lipofundin‐MCT/LCT 20%) and the response was dramatic. Following the fourth monthly course of treatment, pruritis disappeared and the skin lesions showed remarkable objective improvement. PLACK syndrome is a very rare genodermatosis and only six families have been described to date with pathogenic CAST variants. This is the first report of an objective response to a therapeutic agent, which suggests that PLACK is a potentially treatable condition. The remarkable response we report and the relative safety of the intervention should prompt healthcare providers who care for PLACK syndrome patients to explore this as a potential treatment strategy in future studies. [ABSTRACT FROM AUTHOR]

Summary: Synaptic scaffold proteins (e.g., liprin-α, ELKS, RIM, and RIM-BP) orchestrate ion channels, receptors, and enzymes at presynaptic terminals to form active zones for neurotransmitter release. The underlying mechanism of the active zone assembly remains elusive. Here, we report that liprin-α proteins have the potential to oligomerize through the N-terminal coiled-coil region. Our structural and biochemical characterizations reveal that a gain-of-function mutation promotes the self-assembly of the coiled coils in liprin-α2 by disrupting intramolecular interactions and promoting intermolecular interactions. By enabling multivalent interactions with ELKS proteins, the oligomerized coiled-coil region of liprin-α2 enhances the phase separation of the ELKS N-terminal segment. We further show that liprin-α2, by regulating the interplay between two phase separations of ELKS and RIM/RIM-BP, controls the protein distributions. These results imply that the complicated protein-protein interactions allow liprin-α to function with the active zone scaffolds and compartmentalize protein assemblies to achieve comprehensive functions in the active zone.

Background: Gastric cancer (GC) is one of the leading malignant diseases worldwide, especially in Asia. CAST is a potential oncogene in GC carcinogenesis. The character of macrophage infiltration in the GC microenvironment also remains unaddressed. Methods: We first applied machine searching to evaluate gene candidates for GC. CAST expression and pan-cancer surveyance were analyzed using the Human Protein Atlas (HPA) and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) database. The protein–protein interaction (PPI) network was downloaded from STRING. We investigated the impact of CAST on clinical prognosis using a Kaplan–Meier plotter. The correlations between CAST and Lgr5 and macrophage infiltration in GC were determined using TIMER 2.0. Finally, GeneMANIA was also used to evaluate the possible functional linkages between genes. Results: After the machine-assisted search, CAST expression was found to significantly influence the overall survival of GC patients. STRING revealed CAST-related proteomic and transcriptomic associations, mainly concerning the CAPN family. Moreover, CAST significantly impacts the prognosis of GC based on the validation of other datasets. Notably, high CAST expression was correlated with worse overall survival in GC patients (hazard ratio = 1.59; log-rank P = 9.4 × 10−8). CAST and Lgr5 expression were both positively correlated with WNT 2 and WNT 2B. Among the GC patients in several datasets, CAST and macrophage infiltration, evaluated together, showed no obvious association with poor clinical overall survival. Conclusions: CAST plays an important role in the clinical prognosis of GC and is associated with WNT 2/WNT 2B/Lgr5. Our study demonstrates that CAST’s influence on overall survival in GC is regulated by macrophage infiltration.

On August 2, 1995, a police task force led bythe California Department of Industrial Relations raided a compound surrounded by razor wire in El Monte, California. Inside the compound, the police discovered 72 Thai garment workers who had been forced to work 18-hour days for less than $2 an hour. The workers had been held in debt bondage for more than eight years, sewing tirelessly to pay off the cost of their journey to the United States. This paper examines the designing of a model of trauma-informed care for survivors of human trafficking in Los Angeles County.

In vitro – in vivo correlation (IVIVC) allows prediction of in vivo drug deposition from a nasally inhaled drug based on in vitro drug measurements. In vitro measurements include physical particle characterization and, more recently, deposition studies using anatomical models. Currently, there is a lack of IVIVC for deposition measurements in anatomical models, especially for deposition patterns in various nasal cavity regions. Therefore, improvement of in vitro and in vivo measurement methods and knowledge about nasal deposition mechanisms should help IVIVC in the future. In vitro parameters to be considered to predict in vivo nasal drug deposition: Particle characteristics generated by drug delivery devices, patient ventilation and anatomy using anatomical model tools, for example. [Display omitted] [ABSTRACT FROM AUTHOR]

Objective Examination of urine sediment is crucial in acute kidney injury (AKI). In such renal injury, tubular epithelial cells, epithelial cell casts, and dysmorphic red cells may provide clues to etiology. The aim of this study was to compare automated urinalysis findings with manual microscopic analysis in AKI. Methods Samples from patients diagnosed with AKI and control patients were included in the study. Red blood cells, white blood cells, renal tubular epithelial cells/small round cells, casts, and pathologic (path) cast counts obtained microscopically and by a UF1000i cytometer were compared by Spearman test. Logistic regression analysis was used to assess the ability to predict AKI from parameters obtained from the UF1000i. Results There was poor correlation between manual and automated analysis in AKI. None of the parameters could predict AKI using logistic regression analysis. However, the increment in the automated path cast count increased the odds of AKI 93 times. Conclusion Automated urinalysis parameters are poor predictors of AKI, and there is no agreement with manual microscopy. [ABSTRACT FROM AUTHOR]

This study determined the association between Myf5, CAST and MSTN genes and fattening performance traits in 228 (Holstein = 103 and Brown = 125) cattle breeds. Classification and regression tree (CART) was used to determine association between genes and fattening performance. The allele frequencies of Holstein cattle in terms of Myf5, CAST and MSTN genes were A:0.30, B:0.70; A:0.43, G:0.56; A:0.97, B:0.03, respectively, whereas in the Brown Swiss cattle were A:0.36, B:0.64; A:0.65, G:0.34; A:0.88, B:0.12 respectively. The Myf5, CAST, and MSTN genes were found to be in the Hardy–Weinberg equilibrium (p > 0.05) in both of the cattle breeds. As a result, the association between Myf5, CAST and MSTN genes and fattening performance traits were found to be significant according to CART analysis. [ABSTRACT FROM AUTHOR]