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Serological detection of antibodies against SARS-CoV-2 can help establish rates of seroconversion. Here the authors develop a red cell agglutination test to detect antibodies against the receptor binding domain for distribution free of charge to qualified research groups.

Calcifying marine phytoplankton-coccolithophores- are some of the most successful yet enigmatic organisms in the ocean and are at risk from global change. To better understand how they will be affected, we need to know "why" coccolithophores calcify. We review coccolithophorid evolutionary history and cell biology as well as insights from recent experiments to provide a critical assessment of the costs and benefits of calcification. We conclude that calcification has high energy demands and that coccolithophores might have calcified initially to reduce grazing pressure but that additional benefits such as protection from photodamage and viral/bacterial attack further explain their high diversity and broad spectrum ecology. The cost-benefit aspect of these traits is illustrated by novel ecosystem modeling, although conclusive observations remain limited. In the future ocean, the trade-off between changing ecological and physiological costs of calcification and their benefits will ultimately decide how this important group is affected by ocean acidification and global warming.

Current carbon dioxide emissions are an assumed threat to oceanic calcifying plankton (coccolithophores) not just due to rising sea-surface temperatures, but also because of ocean acidification (OA). This assessment is based on single species culture experiments that are now revealing complex, synergistic, and adaptive responses to such environmental change. Despite this complexity, there is still a widespread perception that coccolithophore calcification will be inhibited by OA. These plankton have an excellent fossil record, and so we can test for the impact of OA during geological carbon cycle events, providing the added advantages of exploring entire communities across real-world major climate perturbation and recovery. Here we target fossil coccolithophore groups (holococcoliths and braarudosphaerids) expected to exhibit greatest sensitivity to acidification because of their reliance on extracellular calcification. Across the Paleocene-Eocene Thermal Maximum (56 Ma) rapid warming event, the biogeography and abundance of these extracellular calcifiers shifted dramatically, disappearing entirely from low latitudes to become limited to cooler, lower saturation-state areas. By comparing these range shift data with the environmental parameters from an Earth system model, we show that the principal control on these range retractions was temperature, with survival maintained in high-latitude refugia, despite more adverse ocean chemistry conditions. Deleterious effects of OA were only evidenced when twinned with elevated temperatures.

The US-China trade war forced a reluctant semiconductor industry into someone else’s fight, a very different position from its leading role in the 1980s trade conflict with Japan. This paper describes how the political economy of the global semiconductor industry has evolved since the 1980s. That includes both a shift in the business model behind how semiconductors go from conception to a finished product as well as the geographic reorientation toward Asia of demand and manufactured supply. It uses that lens to explain how, during the modern conflict with China, US policymakers turned to a legally complex set of export restrictions targeting the semiconductor supply chain in the attempt to safeguard critical infrastructure in the telecommunications sector. The potentially far-reaching tactics included weaponization of exports by relatively small but highly specialized American software service and equipment providers in order to constrain Huawei, a Fortune Global 500 company. It describes potential costs of such policies, some of their unintended consequences, and whether policymakers might push them further in the attempt to constrain other Chinese firms.

Abstract Objectives To inform the design of a clinical trial of a targeted screening programme for relatives of individuals affected by thoracic aortic disease, we performed a consensus exercise as to the acceptability of screening, the optimal sequence and choice of tests, long-term patient management, and choice of trial design. Methods Working with the Aortic Dissection Awareness UK & Ireland patient association, we performed a Delphi exercise with clinical experts, patients, and carers, consisting of three rounds of consultation followed by a final multi-stakeholder face-to-face workshop. Results Thirty-five experts and 84 members of the public took part in the surveys, with 164 patients and clinicians attending the final workshop. There was substantial agreement on the need for a targeted screening pathway that would employ a combined approach (imaging + genetic testing). The target population would include the first- and second-degree adult (> 15 years) relatives, with no upper age limit of affected patients. Disagreement persisted about the screening process, sequence, personnel, the imaging method to adopt, computed tomography (CT) scan vs magnetic resonance imaging (MRI), and the specifics of a potential trial, including willingness to undergo randomisation, and measures of effectiveness and acceptability. Conclusion A Delphi process, initiated by patients, identified areas of uncertainty with respect to behaviour, process, and the design of a targeted screening programme for thoracic aortic disease that requires further research prior to any future trial.

The Middle Eocene Climatic Optimum (MECO) at ca. 40 Ma is one of the largest of the transient Eocene global warming events. However, it is relatively poorly known from tropical settings since few sites span the entirety of the MECO event and/or host calcareous microfossils, which are the dominant proxy carrier for palaeoceanographic reconstructions. Ocean Drilling Program (ODP) Pacific Ocean Site 865 in the low-latitude North Pacific (Allison Guyot) has the potential to provide a useful tropical MECO reference, but detailed stratigraphic and chronological constraints needed to evaluate its completeness were previously lacking. We have addressed this deficit by generating new high-resolution biostratigraphic, stable isotope, and X-ray fluorescence (XRF) records spanning the MECO interval (∼38.0–43.0 Ma) in two holes drilled at Site 865. XRF-derived strontium ∕ calcium (Sr∕Ca) and barium ∕ strontium (Ba∕Sr) ratios and Fe count records allow correlation between holes and reveal pronounced rhythmicity, enabling us to develop the first composite section for Holes 865B and 865C and a preliminary cyclostratigraphy for the MECO. Using this new framework, the sedimentary record is interpreted to be continuous across the event, as identified by a pronounced transient benthic foraminiferal δ18O shift of ∼0.8 ‰. Calcareous microfossil biostratigraphic events from widely used zonation schemes are recognized, with generally good agreement between the two holes, highlighting the robustness of the new composite section and allowing us to identify planktic foraminiferal Zones E10–E15 and calcareous nannofossil Zones NP15–18. However, discrepancies in the relative position and ordering of several primary and secondary bioevents with respect to published schemes are noted. Specifically, the stratigraphic highest occurrences of planktic foraminifera, Acarinina bullbrooki, Guembelitrioides nuttalli, and Morozovella aragonensis, and calcareous nannofossils, Chiasmolithus solitus and Sphenolithus furcatolithoides, and the lowest occurrence of Reticulofenestra reticulata all appear higher in the section than would be predicted relative to other bioevents. We also note conspicuous reworking of older microfossils (from planktic foraminiferal Zones E5–E9 and E13) into younger sediments (planktic foraminiferal Zones E14–15) within our study interval consistent with reworking above the MECO interval. Regardless of reworking, the high-quality XRF records enable decimetre-scale correlation between holes and highlight the potential of Site 865 for constraining tropical environmental and biotic changes, not just across the MECO but also throughout the Palaeocene and early-to-middle Eocene interval.

Objetivo. Evaluar la asociación entre la presencia del triángulo hioideo negativo y la respiración oral. Métodos. Se analizó un total de 100 telerradiografías de perfil, 50 de pacientes con respiración nasal y 50 con respiración oral, 70% de pacientes de sexo femenino y 30% masculino. El 59% correspondió a pacientes de entre 20 a 30 años de edad, el 21% de entre 10-20 años, el 15% de entre 30-40 años y el 5% de entre 40-50 años. Los criterios de inclusión fueron fichas clínicas de pacientes que posean telerradiografías de perfil con registro del tipo de respiración (oral o nasal). Los criterios de exclusión fueron fichas clínicas de pacientes con telerradiografía en formato digital, con errores de posicionamiento o dañadas o de sujetos con síndromes genéticos, sin tratamiento de ortodoncia/ ortopedia previos. Se realizó trazado cefalométrico manual (técnica de Rocabado). Se aplicó análisis estadístico descriptivo y prueba estadística Chi2 de Pearson a través de tablas de contingencia. Resultados. Se detectó que existe una asociación estadísticamente significativa (p < 0,001) entre la presencia de un triángulo hioideo negativo y la respiración oral, siendo los pacientes de sexo femenino (p < 0,001) y de 10 a 20 años de edad los que la presentaron con mayor frecuencia (81%). Conclusiones. En la población de estudio analizada existe una asociación estadísticamente significativa entre la presencia de triángulo hioideo negativo y la respiración oral, lo que parece estar relacionado con el sexo y la edad.

ABSTRACT In March 2020, the Rare and Imported Pathogens Laboratory at the UK Health Security Agency (UKHSA) (formerly Public Health England [PHE]) Porton Down, was tasked by the Department of Health and Social Care with setting up a national surveillance laboratory facility to study SARS-CoV-2 antibody responses and population-level sero-surveillance in response to the growing SARS-CoV-2 outbreak. In the following 12 months, the laboratory tested more than 160,000 samples, facilitating a wide range of research and informing UKHSA, DHSC, and UK government policy. Here we describe the implementation and use of the Euroimmun anti-SARS-CoV-2 IgG assay and provide an extended evaluation of its performance. We present a markedly improved overall sensitivity of 91.39% (≥14 days 92.74%, ≥21 days 93.59%) compared to our small-scale early study, and a specificity of 98.56%. In addition, we detail extended characteristics of the Euroimmun assay: intra- and interassay precision, correlation to neutralization, and assay linearity. IMPORTANCE Serology assays have been useful in determining those with previous SARS-CoV-2 infection in a wide range of research and serosurveillance projects. However, assays vary in their sensitivity at detecting SARS-CoV-2 antibodies. Here, we detail an extended evaluation and characterization of the Euroimmun anti-SARS-CoV-2 IgG assay, one that has been widely used within the United Kingdom on over 160,000 samples to date.

Background: Some individuals living with obesity may be relatively metabolically healthy, whilst others suffer from multiple conditions that may be linked to adverse metabolic effects or other factors. The extent to which the adverse metabolic component of obesity contributes to disease compared to the non-metabolic components is often uncertain. We aimed to use Mendelian randomisation (MR) and specific genetic variants to separately test the causal roles of higher adiposity with and without its adverse metabolic effects on diseases. Methods: We selected 37 chronic diseases associated with obesity and genetic variants associated with different aspects of excess weight. These genetic variants included those associated with metabolically ‘favourable adiposity’ (FA) and ‘unfavourable adiposity’ (UFA) that are both associated with higher adiposity but with opposite effects on metabolic risk. We used these variants and two sample MR to test the effects on the chronic diseases. Results: MR identified two sets of diseases. First, 11 conditions where the metabolic effect of higher adiposity is the likely primary cause of the disease. Here, MR with the FA and UFA genetics showed opposing effects on risk of disease: coronary artery disease, peripheral artery disease, hypertension, stroke, type 2 diabetes, polycystic ovary syndrome, heart failure, atrial fibrillation, chronic kidney disease, renal cancer, and gout. Second, 9 conditions where the non-metabolic effects of excess weight (e.g. mechanical effect) are likely a cause. Here, MR with the FA genetics, despite leading to lower metabolic risk, and MR with the UFA genetics, both indicated higher disease risk: osteoarthritis, rheumatoid arthritis, osteoporosis, gastro-oesophageal reflux disease, gallstones, adult-onset asthma, psoriasis, deep vein thrombosis, and venous thromboembolism. Conclusions: Our results assist in understanding the consequences of higher adiposity uncoupled from its adverse metabolic effects, including the risks to individuals with high body mass index who may be relatively metabolically healthy. Funding: Diabetes UK, UK Medical Research Council, World Cancer Research Fund, National Cancer Institute.

I. INTRODUCTION 284 II. THE TRUMP ADMINISTRATION LINKS NATIONAL SECURITY, ECONOMIC SECURITY, AND TRADE POLICY 287 III. U.S. EXPANSION OF EXPORT CONTROLS UNDER EXISTING STATUTES DURING THE TRUMP-CHINA TRADE WAR [...], The Trump administration&apos;s allegations that some imports are a threat to America&apos;s national security have received wide publicity during 2017-20. But the administration was undertaking a more quiet U.S. policy shift on the export side in the same time frame. Addressing the national security threat presented by exports posed different economic and institutional challenges from those associated with import policy, including the acknowledgment that export controls for legitimate national security reasons can be the first-best policy to address the problem at its source. Yet, export controls could also be misused as a beggar-thy-neighbor policy to redistribute economic well-being across countries, even from one ally to another. This paper describes how U.S. export control policy evolved over 2017-20, as well as the international institutions--first the Coordinating Committee for Multilateral Export Controls, then the Wassenaar Arrangement--historically tasked with multilateralizing U.S. export restrictions used to protect national security. With the potential for U.S. export control policy to brush up more frequently against World Trade Organization (WTO) rules designed to limit the use of export restrictions, the paper also highlights new challenges for the WTO&apos;s system of resolving trade disputes. Overall, a U.S. failure to strike the right balance for its export control policy would result in it being ineffective at addressing national security risks, costly for the economy, and problematic for trade and diplomatic relations.