151. Developmental regulation of neuroligand-induced responses in cultured oligodendroglia.
- Author
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Belachew S, Malgrange B, Rigo JM, Rogister B, Coucke P, Mazy-Servais C, and Moonen G
- Subjects
- Acetylcholine pharmacology, Animals, Animals, Newborn, Cells, Cultured, Cerebral Cortex cytology, Cerebral Cortex growth & development, Glutamic Acid pharmacology, Glycine pharmacology, Membrane Potentials drug effects, Patch-Clamp Techniques, Rats, Serotonin pharmacology, Tumor Cells, Cultured, gamma-Aminobutyric Acid pharmacology, Cerebral Cortex drug effects, Neurotransmitter Agents pharmacology, Oligodendroglia drug effects
- Abstract
Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+ concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and metenkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage.
- Published
- 1998
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