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Localized scleroderma (LS) (morphea) is a chronic, inflammatory skin disease with unknown cause that progresses with sclerosis in the skin and/or subcutaneous tissues. Its pathogenesis is not completely understood. Oxidative stress is suggested to have a role in the pathogenesis of localized scleroderma. We have aimed to determine the relationship of morphea lesions with oxidative stress. The total oxidant capacity (TOC), total antioxidant capacity (TAC), paroxonase (PON) and arylesterase (ARES) activity parameters of PON 1 enzyme levels in the serum were investigated in 13 LS patients (generalized and plaque type) and 13 healthy controls. TOC values of the patient group were found higher than the TOC values of the control group ( p < 0.01). ARES values of the patient group was found to be higher than the control group ( p < 0.0001). OSI was significantly higher in the patient group when compared to the control ( p < 0.005). Oxidative stress seems to be effective in the pathogenesis. ARES levels have increased in morphea patients regarding to the oxidative stress and its reduction. Further controlled studies are required in wider series. [ABSTRACT FROM AUTHOR]

Objectives: The purpose of this study was to investigate oxidative stress in children with attention deficit hyperactivity disorder (ADHD). Methods: Total oxidant status (TOS), total antioxidant status (TAS), paraxonase-1 (PON-1) and arylesterase (ARE) activity were measured in 76 children (44 boys, 32 girls) diagnosed with ADHD according to the DSMIV and 78 healthy children (46 boys, 32 girls). Results: Age and sex were similar between the groups (P > 0.05). TOS and the oxidative stress index (OSI) were higher in the patient group than the control group (P < 0.001). PON-1 (P = 0.002), ARE (P = 0.010) activity and TAS (P < 0.001) were lower in the patient group than the control group. Discussion: We found decreased PON-1, ARE activity and TAS, and increased TOS and OSI in children with ADHD. Our study showed that there is significantly increased oxidative stress in children with ADHD. [ABSTRACT FROM AUTHOR]

Aim: Aim of this study is to find out clinical relevance of estimating PON1 arylesterase activity, total oxidative stress (TOS), nitric oxide (NO), and vitamin C levels in maternal serum for prediction of birth weight of newborn. Methods: We have investigated the PON1 arylesterase activity, TOS, NO, vitamin C, total protein, and albumin levels in 56 postnatal clinic patients having newborn weighing <2500 gm (low birth weight) and compared with 56 postnatal clinic patients having newborn weighing >2500 gm. Samples were collected immediately after delivery. Results: PON1 arylesterase activity levels show significant decrease in cases as compared to controls (93.27 ± 13.76 kU/l vs. 112.77 ± 9.42 kU/l). Nitric oxide (nitrate + nitrite) levels are also found to be significantly decreased in cases with respect to controls (22.89 ± 2.65 umol/l vs. 24.73 ± 3.80 umol/l). Total oxidative stress is significantly increased in cases than in control subjects (23.34 ± 2.64 μmol HO equiv./l vs. ± 21.43 ± 2.47 μmol HO equiv/l). Vitamin C levels are also significantly decreased in cases as compared to controls (1.23 ± 0.25 mg/dl vs. 1.34 ± 0.28 mg/dl). Positive correlation between neonatal birth weight and maternal serum PON1 arylesterase activity ( r = 0.682, p < 0.05) while negative correlation is obtained between neonatal birth weight and maternal serum oxidative stress ( r = −0.478, p < 0.05). Logistic regression analysis is applied for assessing predictive utility which demonstrated a significant association of birth weight with PON1 arylesterase activity (AUC = 0.960, Naglekerke's R = 0.793, p < 0.05) Conclusion: Decreased arylesterase activity and antioxidant vitamin C levels with increased total oxidative stress in maternal serum may be considered as the additional risk factors for the development of low birth weight newborn. [ABSTRACT FROM AUTHOR]

Purpose: The metabolic syndrome (MetS) is a constellation of interrelated risk factors of metabolic origin that appear to promote development of cardiovascular disease. It has become one of the most important topics for this decade because of marked increase in cardiovascular disease associated with risk factors. Paraoxonase (PON) is a family of three enzymes, PON1, PON2 and PON3, the gene which is located on chromosome 7. This is high-density lipoprotein (HDL)-associated enzyme with antioxidant activity. The objective of the present study aimed at measurement of PON1 activities in patients with MetS, to compare PON1 activities in patients with MetS with those in controls and assess its relationship with the lipoproteins in serum. Material and Method: Cases were included as per the criteria put forth by the International Diabetes Federation. Serum PON1 activities were measured using spectrophotometer. Statistical analysis was done using SPSS 20.0. Result: Serum PON1 arylesterase and lactonase activities were found to be reduced significantly in patients with MetS than in controls. PON1 activities showed a positive correlation with HDL, fasting blood glucose and diastolic blood pressure. Linear regression analysis showed a significant correlation between PON1 activities and body mass index. Discussion: From the present study, it is clear that reduction in PON1 activities in MetS is mainly due either to abnormalities with synthesis or secretion of HDL cholesterol or oxidative stress as a consequence of excess production of the free radicals. This study also iterates that it is the quality and not the quantity of HDL cholesterol is important while studying the pathophysiology of MetS. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to evaluate serum paraoxonase-1 (PON1) activity and its association with oxidative stress in autoimmune thyroid disease (AITD). Methods: A total of 50 patients with AITD, including 25 with Hashimoto's thyroiditis and 25 with Graves’ disease were enrolled. The control group comprised 27 healthy subjects. Blood samples were obtained in the euthyroid period and 3 months after initiation of medical treatment. Serum samples from patients with AITD and the healthy control group were analyzed for basal PON1, salt-stimulated PON1, and arylesterase (ARE) activities, along with lipid hydroperoxide (LOOH) and total free sulfhydryl (–SH) levels. Results: Serum PON1 activities and –SH levels were significantly lower (P < 0.001, for each), whereas LOOH levels were significantly higher (P < 0.001, for each) in patients with AITD, compared to the control group. We observed no significant differences in ARE levels between the patient and healthy control groups (P > 0.05). PON1 activity was positively correlated with –SH (r = 0.522,P < 0.001) and negatively correlated with LOOH (r = −0.487,P < 0.001). PON1 phenotype distribution of the subjects was not significantly different among the three groups (P = 0.961). Conclusions: Serum PON1 activity is decreased in patients with AITD, and correlated positively with –SH, a well-known antioxidant, and negatively with LOOH, an index of lipid oxidation. [ABSTRACT FROM PUBLISHER]

Introduction: In this study we aimed to detect paraoxonase 1 (PON-1) activity in iron deficiency anemia (IDA) and to compare it with healthy controls by observing the change after iron therapy.Material and Methods: In this study, 50 adult patients with IDA and 40 healthy subjects were enrolled. All patients were analyzed at the beginning and after treatment according to laboratory assessments.Results: Mean paraoxonase and arylesterase activities in the iron deficiency anemia group were significantly lower than mean activities of the control group (102.4 ±19.2 U/l and 163.3 ±13.68 U/l, respectively and 157.3 ±26.4 U/l and 256.1 ±24.6 U/l, respectively; p = 0.0001 for both). Paraoxonase and arylesterase activities significantly increased after treatment for IDA (143.2 ±13.9 and 197.6 ±27.9 U/l, respectively, p = 0.0001). Mean activities after treatment with iron were significantly lower than mean activities in the control group (p = 0.002; p = 0.0001 respectively).Conclusions: Paraoxonase and arylesterase activities in patients with IDA significantly increased after treatment with iron therapy. In adults IDA may also be one of the factors associated with increased risk of atherosclerosis. [ABSTRACT FROM AUTHOR]

β-Thalassemia is the most common hereditary disorder characterized by reduced production of β-globin chains of hemoglobin A (HbA). In recent years, hydroxyurea (HU) has shown promising therapeutic benefits in patients with β-thalassemia by fetal hemoglobin augmentation. We have analyzed effects of hydroxyurea treatment on oxidative stress in β-thalassemia patients by assessing activities of paraoxonase (PON) and arylesterase along with malondialdehyde (MDA) and total reactive oxygen species (ROS) concentrations. Blood samples from 159 individuals including 56 HU-treated and 58 untreated β-thalassemia patients and 45 healthy controls were analyzed. PON activity was found to be highest in healthy individuals (177.76 ± 4.44 U/mL) as compared to treated (52.67 ± 3.65 U/mL) and untreated (55.11 ± 3.26 U/mL) patients. A similar trend was observed in the case of arylesterase activity in normal, β-thalassemia-treated, and untreated (210.0 ± 11.25 U/mL, 163.03 ± 9.04 U/mL, 139.77 ± 10.10 U/mL) subjects. Serum MDA concentrations (2.59 ± 0.09 nmol/mL, 2.45 ± 0.08 nmol/mL, and 1.15 ± 0.05 nmol/mL) and total ROS concentrations (3.73 ± 0.20 nmol/mL, 3.54 ± 0.23 nmol/mL, and 2.45 ± 0.14 nmol/mL) were significantly elevated in both groups (untreated and treated) as compared to healthy individuals ( P < .01). Oxidative stress was found to be markedly elevated in β-thalassemia patients as compared to healthy controls. Insignificant differences were, however, observed in mean concentrations of PON1 paraoxonase and arylesterase activities, serum MDA concentration and total ROS concentrations between HU-treated and untreated patients. We propose that HU therapy alone seems to be ineffective in managing oxidative stress and is likely to offer a better clinical outcome when supplemented with efficient iron chelation therapy and antioxidants. [ABSTRACT FROM AUTHOR]

Objective: Strawberries can improve oxidants-antioxidants balance and reduce some cardiovascular risk factors in obese subjects. Paraoxonase-1 (PON-1) is a high-density lipoprotein-associated enzyme with antioxidant properties that can protect from coronary artery disease in humans. We examined the effect of strawberry consumption on plasma PON-1 activity and lipid profile in healthy nonobese subjects.Methods: Thirty-one subjects (body mass index [BMI] 24.4 ± 4.0 kg/m(2)) on their usual diet consumed 500 g of strawberry pulp daily for 30 days (first course) and after a 10-day washout the cycle was repeated (second course). Fasting blood and spot morning urine samples were collected before, during, and after each strawberry course (8 time points) for determination of paraoxonase and arylesterase PON-1 activities and lipid profile. Twenty subjects served as controls with respect to cholesterol and PON-1 activities changes over the study period.Results: Strawberries decreased mean plasma paraoxonase PON-1 activity and this effect was more evident after the second course (by 11.6%, p < 0.05) than after the first course (5.4%, p = 0.06), whereas arylesterase activity was constant. Strawberries altered total cholesterol levels (p < 0.05) with a tendency to transiently decrease it (by 5.1%) only after 15 days of the first course. Triglycerides and high- and low-density lipoprotein cholesterol did not change in response to fruit consumption. No changes in PON-1 activities and lipid profile were noted in controls. Paraoxonase correlated with arylesterase activity (ƿ from 0.33 to 0.46 at the first 7 time points, p < 0.05). This association disappeared at the end of study (ƿ = 0.07) when the strongest inhibition of paraoxonase was noted.Conclusions: Supplementation of the usual diet with strawberries decreased paraoxonase PON-1 activity and did not improve lipid profiles in healthy nonobese subjects. Further studies are necessary to establish the clinical significance of paraoxonase suppression and to define a group of healthy subjects who can benefit from strawberry consumption with respect to cholesterol levels. [ABSTRACT FROM AUTHOR]

Objective: Altered paraoxonase (PON) and arylesterase (ARE) activities have been shown in anemic chronic kidney disease (CKD) patients and in iron deficiency anemia (IDA) patients. Whether accompanying anemia alone is responsible for this diminished PON and ARE activities in CKD patients or an additive factor for this is not well studied. Therefore, we tried to clarify this issue here.Methods: A total of 82 subjects that consisted of 19 patients with IDA (group 1), 23 anemic CKD patients (group 2), and 40 age and sex matched healthy subjects (group 3) were enrolled. Carotid intima media thickness (CIMT), serum total thiol (–SH), PON, and ARE activities of the participants were analyzed.Results: Group 2 patients had significantly lowest serum levels of Total –SH, PON and ARE. Further comparison showed that total –SH, PON and ARE levels were lower in group 1 than group 3 (p = 0.0001 in both). Regarding comparison of group 1 and 2, only serum ARE levels were significantly lower in group 2 (p = 0.001). PON activity was not different between group 1 and group 2 whereas ARE activity was lower in group 2 than groups 1 and 3. In addition, correlation analysis showed that CIMT was negatively correlated with PON and ARE.Conclusions: This markedly decreased ARE activity in CKD patients, which could not be explained by the anemia alone, may have a role in the pathogenesis of increased atherosclerosis in such patients. Still further studies are needed to certain this. [ABSTRACT FROM PUBLISHER]

The aim of this study is to evaluate the patient stress markers which has the early stage rheumatoid arthritis (RA). Blood samples were collected from the patients diagnosed with rheumatoid arthritis according to American Rheumatism Association ACR 2010 criteria, but not employed the medication of DMARD (Disease-modifying antirheumatic drugs) in order to examine the stress parameters of total oxidant status, total antioxidant status, paraoxonase, paraoxonase NaCl, arylesterase concentration. Twenty nine patients diagnosed with early stage RA and 61 healthy control group included in the study. Peripheral blood samples were examined for all the assays. The average age of the patients was 39.91 ± 13.32 and their average BMI was 28.56 ± 4.15. ESR and CRP values have been found significantly high levels in RA group when we compare with control group. OSI values, which means oxidant parameters, have been found significantly high compared to the control group. When the parameters of arylesterase were compared to the control group, the values of control group were significantly high. Stronger response in patients with early RA suggests that oxidative stress markers may be useful in evaluating the progression of RA as well as in elucidating the mechanisms of disease pathogenesis. [ABSTRACT FROM AUTHOR]

Purpose: In this study, we investigated the role of visfatin in early diabetic nephropathy and its association with oxidative stress, paraoxonase (PON) and arylesterase. Material and Method: Twenty five diabetic patients with microalbuminuria, 25 diabetic patients with normoalbuminuria and 25 healthy individuals were enrolled in the study. Serum total antioxidant status (TAS), total oxidant status (TOS), PON, arylesterase, free sulfhydryl (SH) group, lipid hydroperoxide (LOOH) and visfatin levels were measured. The ratio of TOS to TAS was accepted as oxidative stress index (OSI). Results: Serum visfatin levels were higher in microalbuminuric diabetic group compared to that in normoalbuminuric diabetic group and healthy control group (p<0.001, for each). Visfatin levels in normoalbuminuric diabetic group were significantly higher compared to healthy control group (p=0.001). Correlation analysis yielded that plasma visfatin levels were negatively correlated with SH, arylesterase, and PON levels (r=-0.444, p<0.001; r=-0.340, p=0.004; r=-0.322, p=0.006, respectively) and that they had a positive correlation with LOOH and OSI levels (r=0.252, p=0.034; r=0.622, p<0.001; respectively). According to logistic regression analysis model, the increased levels of OSI and serum visfatin OSI index clarify 51% of developing microalbuminuria in diabetic patients (sensitivity 78.3% and specificity 83.7%). Discussion: The data of this study reveal that increased serum levels of visfatin have a role in the development of diabetic nephropathy. Visfatin has a significant correlation with oxidative stress. [ABSTRACT FROM AUTHOR]

by bacterial opportunist Pseudomonas aeruginosa. The aim of the present study was to evaluate the acute-phase response in experimental Pseudomonas aeruginosa skin infection, by measuring changes in fibrinogen concentration (a positive acute-phase protein, APP) and activity of arylesterase (ARE - a negative APP). We also aimed to evaluate the effect of therapy with phytopreparation Feverfew, containing the active component parthenolide, which has anti-inflammatory properties. Fifteen male mongrel dogs at 2-5 years of age were divided into three groups: group 0 (n=5) with infection induced by subcutaneous injection of bacterial Pseudomonas aeruginosa culture 1×108 CFU/mL; group I (n=5) - infected and treated with Feverfew (standardised extract, active principle parthenolide 0.7% - Nature's Way, USA), by application of 1 capsule at 12-hour intervals. The per os treatment began on post infection hour 4 and continued for 6 days. Dogs from group C (n=5) were controls. Plasma fibrinogen and serum ARE activity were assayed before infection and on 4th, 24th, 48th and 72nd hour and on 7th, 10th and 14th day after infection. The results suggested that fibrinogen levels in dogs from group 0 increased on p.i. hour 24 vs baseline, attained a peak on hour 48 (P<0.001), and persisted high on hour 72. Infected dogs treated with Feverfew (group I) exhibited a similar time course of changes in fibrinogen levels, but the numeric values were lower compared to those of group 0 (P<0.05). ARE activity in experimental animals did not change significantly and was similar to control values. [ABSTRACT FROM AUTHOR]

Introduction: This study was designed and conducted to evaluate the effects of vitamin A, C and E supplementation, and omega-3 fatty acid supplementation on the activity of paraoxonase and arylesterase in an experimental model of diabetes mellitus.Methods: A total of 64 male Sprague Dawley® rats, each weighing 250 g, were randomly distributed into four groups: (a) normal control; (b) diabetic control; (c) diabetic with vitamin A, C and E supplementation; and (d) diabetic with omega-3 fatty acid supplementation. The animals were anaesthetised after four weeks of intervention, and paraoxonase and arylesterase activity in blood plasma, and liver and heart homogenates were measured.Results: Arylesterase activity in the heart and liver homogenates was significantly lower in the diabetic control group than in the normal control group (p < 0.01). Vitamin A, C and E supplementation, and omega-3 fatty acid supplementation significantly increased liver arylesterase activity (p < 0.05). No significant change was observed in paraoxonase activity and other investigated factors.Conclusion: Vitamin A, C and E, or omega-3 fatty acid supplementation were found to increase liver arylesterase activity in streptozotocin-induced diabetic rats. These supplements may be potential agents for the treatment of diabetes mellitus complications. [ABSTRACT FROM AUTHOR]

Objective: The purpose of this study was to investigate paraoxonase (PON) and arylesterase (ARES) enzyme activity in adults with vitamin B12 deficiency, and specific changes in the activities of these enzymes following vitamin B12 treatment. Methods: A total of 46 patients with vitamin B12 deficiency (aged 18–82 years) and 45 healthy volunteer controls (aged 19–64 years) participated in this study. Venous blood samples were collected, and serum vitamin B12, homocysteine (HCY), methylmalonic acid, PON1, and ARES levels were measured. Results: Paired comparison showed that pre- and post-treatment values for PON and ARES were similar between patients and controls (bothP > 0.05). There was no statistically significant relationship between patients’ pre-/post-treatment PON or HCY levels and serum vitamin B12 levels, compared with those of the control group (P > 0.05). Discussion: The results of the present study do not support the hypothesis that the antioxidant enzymes PON and ARES have an underlying role in vitamin B12 deficiency and related hyperhomocysteinemia. Our findings suggest that PON and ARES do not play a role in the systemic effects of vitamin B12 deficiency. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the activities of serum paraoxonase-1 (PON1) and arylesterase (ARE), and the lipid profile in newly diagnosed acute myeloid leukemia (AML) patients. PATIENTS AND METHODS: Thirty-two persons (16 of AML and 16 of healthy control) were included to the study. PON1 and ARE activities were measured as spectrophotometrically in serum samples. High density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol (TC), triglyceride (TG) were analyzed in autoanalyzer. RESULTS: PON1 activities were respectively 16.04 U/L and 18.6 U/L in AML and healthy controls. There was no statistical significance between groups (p > 0.05). The mean ARE activities were respectively 0.21 U/L and 0.36 U/L in AML and healthy controls. Serum ARE activity significantly decreased in AML group (p < 0.001). Serum HDL values were significantly decreased (181.8 ± 76.2 mg/dl; p = 0.002) in AML. There was no difference in total cholesterol, LDL and triglyceride values (respectively; 181.8 ± 76.2 mg/dl, 120.6 ± 64.6 mg/dl, 157.3 ± 87.2 mg/dl; p > 0.05) between AML and controls. CONCLUSIONS: This is the first documented study about serum PON1 activity in AML patients. Although serum PON1 activities were not changed in both groups, our data suggest that the decreased serum ARE activity and HDL levels may be related the pathogenesis of AML. [ABSTRACT FROM AUTHOR]

Zucker fa/fa rats easily develop dyslipidemia and obesity. Restructured pork (RP) is a suitable matrix for including functional ingredients. The effects of glucomannan- RP or glucomannan plus spirulina-enriched RP on plasma lipid/lipoprotein levels, cytochrome P450 7A1 (CYP7A1) expression, and arylesterase activity in growing fa/fa rats fed high-energy, high-fat cholesterol-enriched diets were tested. Groups of six rats each received diet containing 15 % control-RP (C), 15 % glucomannan-RP diet (G), 15 % glucomannan + spirulina-RP diet (GS), and same diets enriched with 2.4 % cholesterol and 0.49 % cholic acid (cholesterol-enriched control (HC), cholesterol-enriched glucomannan (HG), and cholesterol-enriched glucomannan + spirulina (HGS) diets) over a 7-week period. C diet induced obesity, severe hyperglycemia, moderate hypercholesterolemia, and hypertriglyceridemia. Those facts were not significantly modified by G or GS diets. G diet increased CYP7A1 expression but decreased the total cholesterol/high density lipoproteins (HDL)-cholesterol ratio ( p < 0.05) vs. C diet. GS vs. G diet increased ( p < 0.05) CYP7A1 expression. HC vs. C diet reduced food intake, body weight gain, and plasma glucose ( p < 0.01) but increased cholesterolemia ( p < 0.01), lipidemia (plasma cholesterol plus triglycerides) ( p < 0.001), cholesterol/triglyceride ratio in very low density lipoproteins (VLDL), and HDL ( p < 0.05), cholesterol transported by VLDL and intermediate density lipoproteins (IDL) + low density lipoproteins (LDL), total cholesterol/HDL-cholesterol ratio and CYP7A1 expression (at least p < 0.05). HG and HGS diets vs. HC noticeably reduced lipidemia ( p < 0.001), normalized VLDL and IDL + LDL lipid composition, and increased CYP7A1 expression ( p < 0.01) but did not modify the cholesterol/HDL-cholesterol ratio. HGS vs. HG decreased triglyceridemia, the triglyceride-glucose (TyG) index and increased arylesterase/HDL-cholesterol activity ( p < 0.05). In conclusion, G- and GS-RP act as functional foods and notably blocked the dietary cholesterol effects. In addition, HGS-RP improved the glucomannan hypolipidemic effects, increased arylesterase/HDL-cholesterol activity, and decreased insulin resistance. [ABSTRACT FROM AUTHOR]

The paraoxonase (PON) gene family in humans has three members, PON1, PON2, and PON3. Their physiological role(s) and natural substrates are uncertain. We developed a baculovirus-mediated expression system, suitable for all three human PONs, and optimized procedures for their purification. The recombinant PONs are glycosylated with high-mannose-type sugars, which are important for protein stability but are not essential for their enzymatic activities. Enzymatic characterization of the purified PONs has revealed them to be lactonases/lactonizing enzymes, with some overlapping substrates (e.g., aromatic lactones), but also to have distinctive substrate specificities. All three PONs metabolized very efficiently 5-hydroxy-eicosatetraenoic acid 1,5-lactone and 4-hydroxy-docosahexaenoic acid, which are products of both enzymatic and nonenzymatic oxidation of arachidonic acid and docosahexaenoic acid, respectively, and may represent the PONs' endogenous substrates. Organophosphates are hydrolyzed almost exclusively by PON1, whereas bulky drug substrates such as lovastatin and spironolactone are hydrolyzed only by PON3. Of special interest is the ability of the human PONs, especially PON2, to hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria.None of the recombinant PONs protected low density lipoprotein against copper-induced oxidation in vitro.

Oxidative stress is known to be associated with the pathophysiology of chronic kidney disease (CKD). Paraoxonase 1 (PON1) is an antioxidant enzyme that has been proposed as a biomarker for CKD. While several studies have reported an association between serum PON1 activity and CKD, consensus based on systematically analyzed data remains necessary. We set out to conduct a meta-analysis of literature on PON1 in CKD. Electronic databases, such as MEDLINE, Embase and CENTRAL, were searched for available studies on PON1 activity in patients with CKD (without dialysis) as published before December 2022. A random-effects meta-analysis was performed. In total, 24 studies (22 studies on paraoxonase and 11 on arylesterase activity) were eligibly identified. Patients with CKD showed a lower activity of paraoxonase (standard mean difference [SMD], -1.72; 95% confidence interval [CI], -2.15 to -1.29) and arylesterase (SMD, -2.60; 95%CI, -3.96 to -1.24) than healthy controls. In the subgroup analyses, paraoxonase activity was lower in chronic kidney failure (CKF), an advanced stage of CKD, than in non-CKF. In summary, PON1 activity is low in patients with CKD, suggesting that the antioxidant defense by PON1 is impaired in CKD. The decrease in enzyme activity is pronounced in advanced CKD showing some variability depending on the substrate employed to measure PON1 activity. Further studies are warranted.

Background: Mediterranean diet consumption is associated to low prevalence of major degenerative diseases. Low Mediterranean-diet-adherence (MDA) score has been related to high insulin and homeostatic model assessment-insulin resistance levels at birth. The relationship between maternal MDA and offspring lipoprotein profile at birth has been scarcely reported. Methods: Cross-sectional study aimed to study the relationship between pregnancy diet quality and serum lipid, arylesterase and homocysteine values at birth. Cord blood of the offspring of 35 women whose diets were classified as 'adequate' or 'inadequate' according to their 13-point MDA-score (≥7 or <7, respectively) were studied. Results: MDA-scores did not significantly change through pregnancy. Low-MDA-score diets presented a higher atherogenic index, contained less fiber and folates, and had a lower (polyunsaturated + monounsaturated)/saturated fatty acids (PUFA + MUFA/SFA) ratio, more cholesterol, and higher SFA/carbohydrates (SFA/CHO) and ω-6/ω-3 PUFA ratios than their respective high-MDA-score counterparts. Mothers at the low MDA-score delivered neonates with high LDL-c ( P = 0.049), Apo B ( P = 0.040), homocysteine ( P = 0.026) and Apo A1/Apo B ratio ( P = 0.024). Conclusions: Neonates whose mothers consumed low MDA diets presented impaired lipoprotein and increased homocysteine levels at birth. A follow-up study on early cardiovascular disease prevention is needed to understand the importance of present findings later in life. [ABSTRACT FROM AUTHOR]

Background Decreased activity of the enzyme paraoxonase-1 (PON1) has been demonstrated in cardiovascular diseases. Statins, the forefront of pharmacotherapy for dyslipidemia, have been shown to enhance PON1 activity but clinical findings have not been conclusive. Objective To systematically review the clinical findings on the impact of statin therapy on PON1 status (protein concentrations and activities of paraoxonase and arylesterase) and calculate an effect size for the mentioned effects through meta-analysis of available data. Methods Scopus and Medline databases were searched to identify clinical trials. A random-effects model and the generic inverse variance method were used for quantitative data synthesis. Sensitivity analysis was conducted using the one-study remove approach. Random-effects meta-regression was performed to assess the impact of potential confounders on the estimated effect sizes. Results Meta-analysis suggested that statin therapy is associated with a significant elevation of PON1 paraoxonase and arylesterase activities, but not PON1 protein concentration. The PON1-enhancing effects of statins were robust in the sensitivity analyses and were independent of statin dose, treatment duration and changes in plasma low-density lipoprotein cholesterol concentration. Conclusion The increase of paraoxonase and arylesterase activities with statins is a pleiotropic lipid-independent clinical benefit that may partly explain the putative effects of statins in preventing cardiovascular outcomes. [ABSTRACT FROM AUTHOR]

The bleaching of textile fibers with peracetic acid (PAA) results in adequate whiteness at low temperature and neutral pH media. PAA can be added to the bleaching bath in the form of a commercial solution or can be produced in situ in the presence of hydrogen peroxide (H2O2) with the addition of a bleach activator, tetraacetylethylenediamine (TAED), or arylesterase enzymes. In the present study, a knit fabric from regenerated bamboo fibers was bleached with four different PAA bleaching processes: with only PAA, with PAA or H2O2 in combination with TAED, and with H2O2 in combination with arylesterase enzymes. The knit fabric was also bleached conventionally with H2O2 for comparison purposes. Whereas the conventional H2O2 process was carried out at 90℃ and in highly alkaline pH media, the bleaching processes with PAA were carried out at 65℃ and in neutral to slightly alkaline pH media. The bleaching processes with PAA have a strong whitening ability that is comparable to that of the conventional bleaching process with H2O2. The highest whiteness index of the bamboo knit fabrics bleached by different processes was measured after the bleaching process with PAA in combination with TAED (WI 71.2). Overall, with PAA bleaching processes, bamboo knit fabrics with a high degree of whiteness, high water absorbency, and high tenacity can be obtained with low water and energy consumption. [ABSTRACT FROM PUBLISHER]

Objective: Paraoxonase-1 (PON1) 192 QR polymorphism is believed to be an important protective factor for coronary artery disease (CAD); oxidative stress plays a key role in the development of atherosclerotic CAD. Mean platelet volume (MPV) is also central to the processes, including pathophysiology of CAD and endothelial dysfunction. Thus, we aimed to determine the PON1 phenotype, MPV, and oxidative stress parameters in patients with angiographically proven CAD and to compare them with those in healthy subjects. Methods: Fifty-five CAD patients were diagnosed according to the angiography results, and 37 healthy subjects were present in this study. Serum paraoxonase and arylesterase activities were spectrophotometrically measured. Phenotype distribution was evaluated by the salt-stimulated paraoxonase activity according to arylesterase activity. Oxidative stress markers were evaluated by measuring serum total oxidant status (TOS) and total anti-oxidant status (TAS) as well as oxidative stress index. Results: In this study, the ratio of salt-stimulated paraoxonase/ OSI levels (S-PON1/OSI) were lower in the CAD patients and the differences were statistically significant (p<0.05). Therefore, the ratio of salt-stimulated paraoxonase/MPV (S -PON1/MPV) and S- PON1/OSI level were significantly different in the CAD patients as compared with controls group (p<0.01). Conclusion: Our study has suggested that S-PON1/OSI and S-PON1/MPV may play a significant role in CAD. To the best of our knowledge, the present study is the first to study the relationship among PON1 phenotype, MPV, and OSI in CAD patients. Thus, lowering of the oxidative stress and the regulation of MPV strategies may be a promising approach for the treatment of CAD. [ABSTRACT FROM AUTHOR]

In the present study, fabrics constructed from cotton, regenerated bamboo, poly(lactic acid), and soy protein fibers were scoured with pectinase enzymes, bleached with different bleaching processes using peracetic acid (PAA), and conventionally bleached with hydrogen peroxide (HP). The enzymatic scouring and bleaching with PAA have been chosen in order to minimize fiber damage and to perform the processes in more benign conditions. PAA was added to the bleaching bath in the form of a commercial solution or it was produced in situ in the presence of HP with the addition of a bleach activator, tetraacetylethylenediamine (TAED), or arylesterase enzymes. The conventional process was performed at 90 °C in highly alkaline pH media, and the bleaching processes with PAA were performed at 65 °C in neutral to slightly alkaline pH media. The results revealed that after the enzymatic scouring, the hydrophilicity of the fabrics is adequate. Compared with the cotton fibers, the regenerated bamboo and especially the poly(lactic) acid and soy protein fibers are significantly damaged during conventional HP bleaching. By contrast, bleaching with PAA revealed a strong whitening ability that is comparable to that of conventional bleaching with HP but with substantially reduced fiber damage. [ABSTRACT FROM AUTHOR]

Purpose: Thyroid hormones are associated with the oxidant-antioxidant balance in the organism. Hypothyroidism is associated with impaired collagen turnover. We aimed to evaluate paraoxonase (PON) and arylesterase (ARE) activities, which prevent oxidative damage and serum prolidase activity (SPA) which is an important marker of collagen turnover in premenopausal women with subclinical hypothyroidism (SH). Material and Method: Eighteen women with SH (study group) and 18 age-sex-and body mass index-matched controls (control group) were enrolled. PON, ARE and SPA were determined. Results: PON and ARE activities in the study group were significantly lower than in the control group (149.5 (56.2) U/L, 210.5 (161.2) U/L for PON, respectively; p=0.034 and 198.9 (28.6) U/L, 227.0 (101.3) U/L for ARE, respectively; p=0.044). The study group had significantly higher SPA than the control group (670.0 (9.7) U/L, 664.6 (8.2) U/L, respectively; p=0.027). TSH levels positively correlated with SPA (r=0.404, p=0.015) and PON and negatively with ARE (r=-0.348, p=0.037; r=-0.329, p=0.050, respectively). Discussion: Women with SH seem to have higher SPA and lower antioxidant enzyme activities when compared with healthy women which may cause an oxidant status in the organism. The underlying mechanisms and the significance of SPA in development of SH need to be further evaluated. Turk Jem 2015; 19: 38-41 [ABSTRACT FROM AUTHOR]