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The present study analyses the arbitrariness of censorship during Franco's regime. It is pointed out how the works of José Jiménez Lozano are a challenge to the censorship principles: the stories show the consequences of using authority as a deterrent of freedom,as wel l as, the irreducibility of a free conscience. It is based on the unpublished letters regarding José Jiménez Lozano's writings on 1960. In them appears the muddled warnings issued by the political authority, following the advice of the ecclesial authority,against his articles. Secondly, the present work studies the censo rship files regarding the three first novels of the writer: Historia de un otoño (1971), El sambenito (1972) and La salamandra (1973), which are kept at Archivo General de la Administración. The censorshipau thority urges to maintain Spain's image of a country of one faith and one thought.

Introducción: La presencia en la atmósfera del material particulado ocasiona variedad de impactos a la vegetación, materiales y el hombre. Las emisiones principales de la textilera “Desembarco del Granma” provienen de la quema de fuel oil en los calentadores de aceite y en las calderas generadoras de vapor. Las emisiones se pueden estimar por medio de los factores de emisión. Objetivo: Determinar la propuesta tecnológica necesaria para reducir el impacto a la calidad del aire provocado por el material particulado generado por los procesos auxiliares de combustión en la UB “Desembarco del Granma”. Materiales y Métodos: Se emplearon los factores de emisión. La modelación de la dispersión de PM10 se realizó mediante el software especializado del Centro Meteorológico Provincial, basado en un modelo de pluma Gaussiano. Se utilizó el software Aspen Plus 8.0. Resultados y Discusión: Se estimaron las emisiones de PM10 y PM2.5. Las inmisiones de PM10 alcanzan valores máximos de 100 μg/m3, para una categoría de calidad del aire de mala al superar lo establecido por la NC 1020 (2014). Con el Aspen Plus se determinaron las características que tendrán los ciclones a agregar a las fuentes fijas consideradas. Se seleccionó el tipo Stairmand-HE. Conclusiones: La propuesta para mitigar el impacto ambiental del material particulado consiste en instalar dos ciclones, uno en el generador de vapor y otro en el calentador de aceite de la planta de servicios de Ingeniería de la UB “Desembarco del Granma”.

Resumen: Introducción: La fractura de los primeros pasos o fractura de toddler es una fractura espiroidea de tibia propia de la primera infancia. El objetivo es analizar su incidencia y el manejo diagnóstico y terapéutico actual. Pacientes y métodos: Estudio descriptivo retrospectivo de los pacientes de 0 a 3 años diagnosticados en un hospital terciario entre los años 2013 y 2017. Resultados: Registrados 53 pacientes (10,6 casos/año), con una mediana de edad de 2 años y ligero predominio masculino. La radiografía inicial resultó normal en el 24,5%. Con la aproximación inicial, el 69,8% de los pacientes se diagnosticaron de fractura, el 11,3% de sospecha de fractura y el 18,9% de contusión. El 22% precisaron prueba de control; 10 radiografía (patológica 90%) y 5 ecografía (patológica 80%, 3 de ellos con radiografía inicial normal). El 80,8% de los pacientes se inmovilizaron con férula frente al 19,2% en los que se realizó inmovilización flexible o no inmovilización. Se encontraron complicaciones en el 21,4% de los pacientes inmovilizados con férula, fundamentalmente úlceras por presión (19%), que fueron más frecuentes en este grupo que en los no inmovilizados (21,4 vs. 0%; p = 0,006), sin diferencias significativas en cuanto a tiempo hasta carga. Conclusiones: La radiografía simple tiene una sensibilidad limitada para el diagnóstico de la fractura de los primeros pasos. En el grupo de pacientes con radiografía normal el uso de ecografía puede contribuir al diagnóstico y a evitar radiación adicional. Aunque el tratamiento más común de esta fractura sigue siendo la inmovilización con férula, la alternativa sin inmovilización rígida no parece obtener peores resultados, incluso parece presentar menor morbilidad asociada al tratamiento. Abstract: Introduction: Toddler's fracture is an accidental spiral tibial fracture, characteristic of the early childhood. The objective of this study is to determine the incidence and current diagnosis and management of this disorder. Patients and methods: A retrospective study was conducted on a sample of patients aged 0-3 years diagnosed with a toddler's fracture in a tertiary hospital between years 2013 and 2017. Results: A total of 53 patients were registered (10.6 cases per year). The median age was 2 years, with a slight male predominance. The initial radiograph was normal in 24.5% of patients. With the initial approach, 69.8% of patients were diagnosed with fracture, 11.3% with suspected fracture, and 18.9% with contusion. A follow-up was required in 22% required a control test, using radiographs in 10 patients (pathological 90%), and ultrasound in 5 (pathological 80%, 3 of them with normal initial radiography). The large majority (80.8%) of the patients were immobilised with a cast, while flexible immobilisation or non-immobilisation was used in 19.2%. Complications were found in a 21.4% of patients immobilised with splint, mainly skin injuries (19%). These were more frequent in this group than in those that were not immobilised (21.4% vs. 0%, P = .006); with no significant differences in time to weight-bearing. Conclusions: Radiography has a limited sensitivity for the diagnosis of toddler's fracture. In the group of patients with normal radiography, the use of ultrasound can be helpful to the diagnosis and avoid additional radiation. Even though the most common treatment continues to be immobilisation with a splint, the alternative without rigid immobilisation does not seem to give worse results, even with lower morbidity associated with the treatment.

Introduction: Toddler's fracture is an accidental spiral tibial fracture, characteristic of the early childhood. The objective of this study is to determine the incidence and current diagnosis and management of this disorder. Patients and methods: A retrospective study was conducted on a sample of patients aged 0–3 years diagnosed with a toddler's fracture in a tertiary hospital between years 2013 and 2017. Results: A total of 53 patients were registered (10.6 cases per year). The median age was 2 years, with a slight male predominance. The initial radiograph was normal in 24.5% of patients. With the initial approach, 69.8% of patients were diagnosed with fracture, 11.3% with suspected fracture, and 18.9% with contusion. A follow-up was required in 22% through a control test, using radiographs in 10 patients (pathological 90%), and ultrasound in 5 (pathological 80%, 3 of them with normal initial radiography). The large majority (80.8%) of the patients were immobilised with a cast, while flexible immobilisation or non-immobilisation was used in 19.2%. Complications were found in a 21.4% of patients immobilised with splint, mainly skin injuries (19%). These were more frequent in this group than in those that were not immobilised (21.4% vs. 0%, P = .006); with no significant differences in time to weight-bearing. Conclusions: Radiography has a limited sensitivity for the diagnosis of toddler's fracture. In the group of patients with normal radiography, the use of ultrasound can be helpful to the diagnosis and avoid additional radiation. Even though the most common treatment continues to be immobilisation with a splint, the alternative without rigid immobilisation does not seem to give worse results, even with lower morbidity associated with the treatment. Resumen: Introducción: La fractura de los primeros pasos o fractura de toddler es una fractura espiroidea de tibia propia de la primera infancia. El objetivo es analizar su incidencia y el manejo diagnóstico y terapéutico actual. Pacientes y métodos: Estudio descriptivo retrospectivo de los pacientes de 0 a 3 años diagnosticados en un hospital terciario entre los años 2013 y 2017. Resultados: Registrados 53 pacientes (10,6 casos/año), con una mediana de edad de 2 años y ligero predominio masculino. La radiografía inicial resultó normal en el 24,5%. Con la aproximación inicial, el 69,8% de los pacientes se diagnosticaron de fractura, el 11,3% de sospecha de fractura y el 18,9% de contusión. El 22% precisaron prueba de control; 10 radiografía (patológica 90%) y 5 ecografía (patológica 80%, 3 de ellos con radiografía inicial normal). El 80,8% de los pacientes se inmovilizaron con férula frente al 19,2% en los que se realizó inmovilización flexible o no inmovilización. Se encontraron complicaciones en el 21,4% de los pacientes inmovilizados con férula, fundamentalmente úlceras por presión (19%), que fueron más frecuentes en este grupo que en los no inmovilizados (21,4 vs. 0%; p = 0,006), sin diferencias significativas en cuanto a tiempo hasta carga. Conclusiones: La radiografía simple tiene una sensibilidad limitada para el diagnóstico de la fractura de los primeros pasos. En el grupo de pacientes con radiografía normal el uso de ecografía puede contribuir al diagnóstico y a evitar radiación adicional. Aunque el tratamiento más común de esta fractura sigue siendo la inmovilización con férula, la alternativa sin inmovilización rígida no parece obtener peores resultados, incluso parece presentar menor morbilidad asociada al tratamiento.

Abstract Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased replication intermediates from Saccharomyces cerevisiae, we orient 58,651 replication tracks reproducing population-based replication directionality profiles and map 4964 and 4485 individual initiation and termination events, respectively. Although most events cluster at known origins and fork merging zones, 9% and 18% of initiation and termination events, respectively, occur at many locations previously missed. Thus, FORK-seq reveals the full extent of cell-to-cell heterogeneity in DNA replication.

The selection of plant genotypes with improved productivity and tolerance to environmental constraints has always been a major concern in plant breeding. Classical approaches based on the generation of variability and selection of better phenotypes from large variant collections have improved their efficacy and processivity due to the implementation of molecular biology techniques, particularly genomics, Next Generation Sequencing and other omics such as proteomics and metabolomics. In this regard, the identification of interesting variants before they develop the phenotype trait of interest with molecular markers has advanced the breeding process of new varieties. Moreover, the correlation of phenotype or biochemical traits with gene expression or protein abundance has boosted the identification of potential new regulators of the traits of interest, using a relatively low number of variants. These important breakthrough technologies, built on top of classical approaches, will be improved in the future by including the spatial variable, allowing the identification of gene(s) involved in key processes at the tissue and cell levels.

The present study examined the mediating role of depression symptoms in the relation of college stress and minority status stress to cumulative grade point average (GPA) and persistence intentions among Hispanic women (n = 426) enrolled in a 4-year major research public institution. Results of path analyses indicated a strong model-data fit for the proposed model. When controlling for each other, both typical college stress and minority status stress positively predicted depression and, in turn, depression negatively predicted college persistence intentions. Tests of indirect effects revealed that depression mediated the relation of college stress and minority status stress to persistence. No mediation effects were present in the relation of the stress variables to cumulative GPA. Findings suggest that depression symptoms is one of the pathways through which college stress and minority status stress may result in lower levels of persistence intentions and, possibly, dropout behavior among Hispanic college women in 4-year institutions.

Pine wilt disease (PWD), caused by the plant–parasitic nematode Bursaphelenchus xylophilus, has become a severe environmental problem in the Iberian Peninsula with devastating effects in Pinus pinaster forests. Despite the high levels of this species' susceptibility, previous studies reported heritable resistance in P. pinaster trees. Understanding the basis of this resistance can be of extreme relevance for future programs aiming at reducing the disease impact on P. pinaster forests. In this study, we highlighted the mechanisms possibly involved in P. pinaster resistance to PWD, by comparing the transcriptional changes between resistant and susceptible plants after infection. Our analysis revealed a higher number of differentially expressed genes (DEGs) in resistant plants (1,916) when compared with susceptible plants (1,226). Resistance to PWN is mediated by the induction of the jasmonic acid (JA) defense pathway, secondary metabolism pathways, lignin synthesis, oxidative stress response genes, and resistance genes. Quantification of the acetyl bromide-soluble lignin confirmed a significant increase of cell wall lignification of stem tissues around the inoculation zone in resistant plants. In addition to less lignified cell walls, susceptibility to the pine wood nematode seems associated with the activation of the salicylic acid (SA) defense pathway at 72 hpi, as revealed by the higher SA levels in the tissues of susceptible plants. Cell wall reinforcement and hormone signaling mechanisms seem therefore essential for a resistance response.

Regulatory T cells are a population of CD4+ T cells that play a critical role in peripheral tolerance and control of immune responses to pathogens. The purpose of this study was to measure the percentages of two different regulatory T cells subpopulations,

Regulatory T cells are a population of CD4+ T cells that play a critical role in peripheral tolerance and control of immune responses to pathogens. The purpose of this study was to measure the percentages of two different regulatory T cells subpopulations, identified by the presence or absence of CD31 (Recent thymic emigrants and peripherally induced naïve regulatory T cells), in term and preterm infant cord blood. We report the association of prenatal factors, intrauterine exposure to lipopolysaccharide and inflammation and the percentages of these regulatory T cell subpopulations in term and preterm infants. Cord blood samples were collected from both term and preterm infants and mononuclear cells isolated over a Ficoll-Hypaque cushion. Cells were then stained with fluorochrome-labeled antibodies to characterize regulatory T cell populations and analyzed with multi-color flow cytometry. Cord blood plasma C-reactive protein, and lipopolysaccharide were also measured. Placental pathology was also examined. We report a gestational age-dependent difference in the percentage of total regulatory T cells, in which preterm infants of lower gestational ages have an increased percentage of regulatory T cells. We report the presence of two populations of regulatory T cells (CD31+ and CD31-) in cord blood of term and preterm infants and their association with different maternal and fetal characteristics. Factors associated with differences in the percentage of CD31- Tregs included the use of prenatal antibiotics, steroids and magnesium sulfate. In addition, the percentage of CD31- Tregs was significantly higher in cord blood of preterm pregnancies associated with inflammation and prenatal lipopolysaccharide exposure. The peripheral Treg pool of preterm infants could be altered by prenatal exposure to inflammation and chorioamnionitis; however, the clinical implications of this finding are not yet understood.

The text is a review of two books. The first one is "Le Sentiment de puissance dans la philosophie de Friedrich Nietzsche" by David Simonin, which analyzes the notion of the feeling of power in Nietzsche's philosophy. The author reconstructs historically this notion and explores its relationship with affectivity and action. The second book is "Política, historia, conciencia" by Eric Voegelin, a compilation of essays and fragments that cover the work of the German philosopher. Both books offer a deep and innovative insight into the topics they address. [Extracted from the article]

Introduction: In the treatment of waste water at the "Desembarco del Granma" textile factory there is a neutralization tank, which resembles a continuous reactor with agitation. This equipment uses H2SO4, which must be replaced by a chemical agent with a lower impact on the environment. Objective: To determine the viability of using the existing reactor at the "Desembarco del Granma" textile factory for the neutralization of residual water when using CO2. Materials and Methods: Continuous stirred reactors were compared at laboratory and industrial levels, where the criterion of geometric similarity was considered. The Aspen Plus program was used to simulate the industrial reactor, considering the kinetics obtained at the laboratory level. The fluid package selected was the UNIQUAC. Results and Discussion: The speed at the tip of the impeller is the same for both reactors and the relationship between the diameter of the impeller and that of the tank remains practically constant, so there is geometric similarity between the reactors. The pH of the residual water neutralized with CO2 was 7.2, showing adequate neutralization. Conclusions: Geometric similarity was evidenced between continuous stirred reactors at laboratory and industrial levels. The pH of the neutralized wastewater in the industrial reactor, determined by simulation, was 7.2. It was proven, with the use of Aspen Plus, that the textile company's industrial reactor can be used for the wastewater neutralization process when CO2 is used instead of H2SO4. [ABSTRACT FROM AUTHOR]

SUMMARY: Crops often have to face several abiotic stresses simultaneously, and under these conditions, the plant's response significantly differs from that observed under a single stress. However, up to the present, most of the molecular markers identified for increasing plant stress tolerance have been characterized under single abiotic stresses, which explains the unexpected results found when plants are tested under real field conditions. One important regulator of the plant's responses to abiotic stresses is abscisic acid (ABA). The ABA signaling system engages many stress‐responsive genes, but many others do not respond to ABA treatments. Thus, the ABA‐independent pathway, which is still largely unknown, involves multiple signaling pathways and important molecular components necessary for the plant's adaptation to climate change. In the present study, ABA‐deficient tomato mutants (flacca, flc) were subjected to salinity, heat, or their combination. An in‐depth RNA‐seq analysis revealed that the combination of salinity and heat led to a strong reprogramming of the tomato transcriptome. Thus, of the 685 genes that were specifically regulated under this combination in our flc mutants, 463 genes were regulated by ABA‐independent systems. Among these genes, we identified six transcription factors (TFs) that were significantly regulated, belonging to the R2R3‐MYB family. A protein–protein interaction network showed that the TFs SlMYB50 and SlMYB86 were directly involved in the upregulation of the flavonol biosynthetic pathway‐related genes. One of the most novel findings of the study is the identification of the involvement of some important ABA‐independent TFs in the specific plant response to abiotic stress combination. Considering that ABA levels dramatically change in response to environmental factors, the study of ABA‐independent genes that are specifically regulated under stress combination may provide a remarkable tool for increasing plant resilience to climate change. [ABSTRACT FROM AUTHOR]

Adoptive cell therapy using virus‐specific T cells (VST) is a strategy for treating common opportunistic viral infections after transplantation, particularly when these infections do not resolve through antiviral drug therapy. The availability of third‐party healthy donors allows for the immediate use of cells for allogeneic therapy in cases where patients lack an appropriate donor. Here, we present the creation of a cell donor registry of human leukocyte antigen (HLA)‐typed blood donors, REDOCEL, a strategic initiative to ensure the availability of compatible cells for donation when needed. Currently, the registry consists of 597 healthy donors with a median age of 29 years, 54% of whom are women. The most represented blood groups were A positive and O positive, with 36.52% and 34.51%, respectively. Also, donors were screened for cytomegalovirus (CMV) and Epstein–Barr virus (EBV). Almost 65% of donors were CMV‐seropositive, while less than 5% were EBV‐seronegative. Of the CMV‐seropositive donors, 98% were also EBV‐seropositive. High‐resolution HLA‐A, ‐B, ‐C, ‐DRB1 and ‐DQB1 allele and haplotype frequencies were determined in the registry. Prevalent HLA alleles and haplotypes were well represented to ensure donor‐recipient HLA‐matching, including alleles reported to present viral immunodominant epitopes. Since the functional establishment of REDOCEL, in May 2019, 87 effective donations have been collected, and the effective availability of donors with the first call has been greater than 75%. Thus, almost 89% of patients receiving an effective donation had available at least 5/10 HLA‐matched cell donors (HLA‐A, ‐B, ‐C, ‐DRB1, and ‐DQB1). To summarize, based on our experience, a cell donor registry from previously HLA‐typed blood donors is a useful tool for facilitating access to VST therapy. [ABSTRACT FROM AUTHOR]

Different environmental and developmental cues involve low oxygen conditions, particularly those associated to abiotic stress conditions. It is widely accepted that plant responses to low oxygen conditions are mainly regulated by ethylene (ET). However, interaction with other hormonal signaling pathways as gibberellins (GAs), auxin (IAA), or nitric oxide (NO) has been well-documented. In this network of interactions, abscisic acid (ABA) has always been present and regarded to as a negative regulator of the development of morphological adaptations to soil flooding: hyponastic growth, adventitious root emergence, or formation of secondary aerenchyma in different plant species. However, recent evidence points toward a positive role of this plant hormone on the modulation of plant responses to hypoxia and, more importantly, on the ability to recover during the post-hypoxic period. In this work, the involvement of ABA as an emerging regulator of plant responses to low oxygen conditions alone or in interaction with other hormones is reviewed and discussed.

Soil flooding is a compound abiotic stress that alters soil properties and limits atmospheric gas diffusion (O2 and CO2) to the roots. The involvement of abscisic acid (ABA) in the regulation of soil flooding-specific genetic and metabolic responses has been scarcely studied despite its key importance as regulator in other abiotic stress conditions. To attain this objective, wild type and ABA-deficient tomatoes were subjected to short-term (24 h) soil waterlogging. After this period, gas exchange parameters were reduced in the wild type but not in ABA-deficient plants that always had higher E and gs. Transcript and metabolite alterations were more intense in waterlogged tissues, with genotype-specific variations. Waterlogging reduced the ABA levels in the roots while inducing PYR/PYL/RCAR ABA receptors and ABA-dependent transcription factor transcripts, of which induction was less pronounced in the ABA-deficient genotype. Ethylene/O2-dependent genetic responses (ERFVIIs, plant anoxia survival responses, and genes involved in the N-degron pathway) were induced in hypoxic tissues independently of the genotype. Interestingly, genes encoding a nitrate reductase and a phytoglobin involved in NO biosynthesis and scavenging and ERFVII stability were induced in waterlogged tissues, but to a lower extent in ABA-deficient tomato. At the metabolic level, flooding-induced accumulation of Ala was enhanced in ABA-deficient lines following a differential accumulation of Glu and Asp in both hypoxic and aerated tissues, supporting their involvement as sources of oxalacetate to feed the tricarboxylic acid cycle in waterlogged tissues and constituting a potential advantage upon long periods of soil waterlogging. The promoter analysis of upregulated genes indicated that the production of oxalacetate from Asp via Asp oxidase, energy processes such as acetyl-CoA, ATP, and starch biosynthesis, and the lignification process were likely subjected to ABA regulation. Taken together, these data indicate that ABA depletion in waterlogged tissues acts as a positive signal, inducing several specific genetic and metabolic responses to soil flooding.