151. Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors.
- Author
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Sørensen OE, Cowland JB, Theilgaard-Mönch K, Liu L, Ganz T, and Borregaard N
- Subjects
- Acute-Phase Proteins biosynthesis, Acute-Phase Proteins genetics, Antimicrobial Cationic Peptides genetics, Cathelicidins, Cell Division drug effects, Cell Division physiology, Cells, Cultured, Cytokines pharmacology, Fibroblast Growth Factor 2 pharmacology, Humans, Inflammation Mediators pharmacology, Insulin-Like Growth Factor I pharmacology, Keratinocytes cytology, Lipocalin-2, Lipocalins, Oncogene Proteins biosynthesis, Oncogene Proteins genetics, Organ Culture Techniques, Protein Biosynthesis, Proteinase Inhibitory Proteins, Secretory, Proteins genetics, Proto-Oncogene Proteins, RNA, Messenger biosynthesis, Transforming Growth Factor alpha pharmacology, Transforming Growth Factor beta pharmacology, Transforming Growth Factor beta1, Wound Healing drug effects, Wound Healing genetics, beta-Defensins biosynthesis, beta-Defensins genetics, Anti-Infective Agents metabolism, Antimicrobial Cationic Peptides biosynthesis, Growth Substances physiology, Keratinocytes metabolism, Keratinocytes microbiology, Wound Healing physiology
- Abstract
In addition to acting as a physical barrier against microorganisms, the skin produces antimicrobial peptides and proteins. After wounding, growth factors are produced to stimulate the regeneration of tissue. The growth factor response ceases after regeneration of the tissue, when the physical barrier protecting against microbial infections is re-established. We found that the growth factors important in wound healing, insulin-like growth factor I and TGF-alpha, induce the expression of the antimicrobial peptides/polypeptides human cationic antimicrobial protein hCAP-18/LL-37, human beta-defensin 3, neutrophil gelatinase-associated lipocalin, and secretory leukocyte protease inhibitor in human keratinocytes. Both an individual and a synergistic effect of these growth factors were observed. These findings offer an explanation for the expression of these peptides/polypeptides in the skin disease psoriasis and in wound healing and define a host defense role for growth factors in wound healing.
- Published
- 2003
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