10,238 results on '"A. Cauchois"'
Search Results
152. Mass characterization of multi-pixel photon counters for the T2K 280 m near detector
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Moreau, F., Vanel, J.-C., Drapier, O., Gonin, M., Bonnemaison, A., Cauchois, A., Geerebaert, Y., and Couturier-Le Quellec, S.
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- 2010
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153. COVID-19 as a potential trigger of complement-mediated atypical HUS
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El Sissy, Carine, Saldman, Antonin, Zanetta, Gilbert, Martins, Paula Vieira, Poulain, Coralie, Cauchois, Raphaël, Kaplanski, Gilles, Venetz, Jean-Pierre, Bobot, Mickaël, Dobosziewicz, Hélène, Daniel, Laurent, Koubi, Marie, Sadallah, Salima, Rotman, Samuel, Mousson, Christiane, Pascual, Manuel, Frémeaux-Bacchi, Véronique, and Fakhouri, Fadi more...
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- 2021
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154. A Natural Polyphenol Exerts Antitumor Activity and Circumvents Anti–PD-1 Resistance through Effects on the Gut Microbiota
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Messaoudene, Meriem, primary, Pidgeon, Reilly, additional, Richard, Corentin, additional, Ponce, Mayra, additional, Diop, Khoudia, additional, Benlaifaoui, Myriam, additional, Nolin-Lapalme, Alexis, additional, Cauchois, Florent, additional, Malo, Julie, additional, Belkaid, Wiam, additional, Isnard, Stephane, additional, Fradet, Yves, additional, Dridi, Lharbi, additional, Velin, Dominique, additional, Oster, Paul, additional, Raoult, Didier, additional, Ghiringhelli, François, additional, Boidot, Romain, additional, Chevrier, Sandy, additional, Kysela, David T., additional, Brun, Yves V., additional, Falcone, Emilia Liana, additional, Pilon, Geneviève, additional, Oñate, Florian Plaza, additional, Gitton-Quent, Oscar, additional, Le Chatelier, Emmanuelle, additional, Durand, Sylvere, additional, Kroemer, Guido, additional, Elkrief, Arielle, additional, Marette, André, additional, Castagner, Bastien, additional, and Routy, Bertrand, additional more...
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- 2022
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155. Membership Inference Attacks on Aggregated Time Series with Linear Programming
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Voyez, Antonin, primary, Allard, Tristan, additional, Avoine, Gildas, additional, Cauchois, Pierre, additional, Fromont, Elisa, additional, and Simonin, Matthieu, additional
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- 2022
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156. What is missing for large scale deployment of CCS in Europe?
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Simon, Manon, primary, Cauchois, Gaelle, additional, Lockwood, Toby, additional, and Isaenko, Anastasia, additional
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- 2022
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157. Production of biogas with CCS in Norway
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Gentile, Valentin, primary, Isaenko, Anastasia, additional, Cauchois, Gaelle, additional, Voss, Kaja, additional, and Carpenter, Mike, additional
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- 2022
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158. Blue H2 and CCUS in Ukraine
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Cauchois, Gaelle, primary, Venugopal, Malavika, additional, Gassan-zade, Olga, additional, Kornienko, Hanna, additional, and Beck, Bendan, additional
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- 2022
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159. Aortic Valve Insufficiency Related to Eosinophilic Granulomatosis with Polyangiitis
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Cauchois, Raphael, primary, Renard, Sebastien, additional, Kaplanski, Gilles, additional, and Jarrot, Pierre-Andre, additional
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- 2022
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160. COVID-19 as a potential trigger of complement-mediated atypical HUS
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Gilles Kaplanski, Antonin Saldman, Hélène Dobosziewicz, Christiane Mousson, Fadi Fakhouri, Manuel Pascual, Carine El-Sissy, Samuel Rotman, Laurent Daniel, Mickaël Bobot, Salima Sadallah, Véronique Frémeaux-Bacchi, Coralie Poulain, Jean-Pierre Venetz, Paula Vieira-Martins, Gilbert Zanetta, Marie Koubi, Raphael Cauchois, Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), CHU Amiens-Picardie, Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lausanne = University of Lausanne (UNIL), Hôpital Bicêtre, Hôpital de la Timone [CHU - APHM] (TIMONE), Aix Marseille Université (AMU), Assistance Publique - Hôpitaux de Marseille (APHM), Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), École pratique des hautes études (EPHE), and RANCHON, GUILLAUME more...
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2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,[SDV]Life Sciences [q-bio] ,Immunology ,Cell Biology ,Hematology ,Biochemistry ,Virology ,Complement (complexity) ,[SDV] Life Sciences [q-bio] ,Medicine ,business ,Letter to Blood ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience; No abstract available
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- 2021
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161. Attaque par inférence d'appartenance sur des séries temporelles agrégées en utilisant la programmation par contraintes
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Antonin Voyez, Tristan Allard, Gildas Avoine, Pierre Cauchois, Elisa Fromont, Matthieu Simonin, Large Scale Collaborative Data Mining (LACODAM), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Enedis, Declarative & Reliable management of Uncertain, user-generated Interlinked Data (DRUID), GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut National des Sciences Appliquées (INSA), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), the World Is Distributed Exploring the tension between scale and coordination (WIDE), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-SYSTÈMES LARGE ÉCHELLE (IRISA-D1), Service Expérimentation et Développement (SED [Rennes]), Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Security & PrIvaCY (SPICY), and SYSTÈMES LARGE ÉCHELLE (IRISA-D1) more...
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[INFO.INFO-LG]Computer Science [cs]/Machine Learning [cs.LG] ,[INFO.INFO-AI]Computer Science [cs]/Artificial Intelligence [cs.AI] - Abstract
International audience; L’agrégation est largement utilisée comme méthode de protection de la vie privée. Les attaques par inférence d’appartenance sur agrégat ont pour but de déterminer si une cible donnée a participé ou non au calcul de l’agrégat attaqué. Dans cet article, nous étudions la vulnérabilé de séries temporelles agrégées - où chaque point est un agrégat horodaté - face à des attaques par inférence d’appartenance. L’attaquant que nous considérons dispose de connaissances auxiliaires sur un sur-ensemble des données agrégées (e.g., issu d’une fuite de données). Nous proposons une nouvelle attaque tirant parti de ce type de connaissances auxiliaires et des multiples points formant la série temporelle agrégat. Notre attaque est modélisée comme un problème d’optimisation linéraire en nombres entiers, permettant à l’attaquant de bénéficier de la puissance des solveurs dédiés (e.g., Gurobi). Cette attaque, testée sur des jeux de données publics, montre la vulnérabilité d’une publication de série temporelle agrégat si le nombre de séries agrégées est trop faible face au nombre de points constituant la série. more...
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- 2021
162. Dryland Horticulture in Maupiti: An Ethnoarchaeological Study
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CAUCHOIS, MICKAELLE-HINANUI
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- 2002
163. Outcome of Critically Ill COVID-19 Patients According to the Setting of Corticosteroid Initiation—A Retrospective Observational Cohort Study
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Voicu, Sebastian, primary, Lacoste-Palasset, Thomas, additional, Malissin, Isabelle, additional, Bekhit, Shana, additional, Cauchois, Eléonore, additional, Dahmani, Sirine, additional, Saib, Melkir, additional, Grant, Caroline, additional, Naim, Giulia, additional, M’Rad, Aymen, additional, Pepin-Lehaleur, Adrien, additional, Ekhérian, Jean-Michel, additional, Deye, Nicolas, additional, and Mégarbane, Bruno, additional more...
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- 2021
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164. Syndrome catastrophique des antiphospholipides traité par éculizumab : à propos de deux cas
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Le Roux, A., primary, Cauchois, R., additional, Roumieu, V., additional, Jean, R., additional, Mazodier, K., additional, and Kaplanski, G., additional
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- 2021
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165. Early IL-1 receptor blockade in severe inflammatory respiratory failure complicating COVID-19
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Raphael Cauchois, Karin Mazodier, Valery Benjamin Blasco, David Delarbre, Marie Koubi, Vincent Pestre, Pierre André Jarrot, Cecile Manet, Julien Carvelli, Louis Fouche, Charleric Bornet, Charles A. Dinarello, Gilles Kaplanski, Rodolphe Jean, and Vanessa Pauly more...
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0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] ,Systemic inflammation ,Gastroenterology ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Inflammation ,Internal medicine ,medicine ,pneumonia ,Adverse effect ,Mechanical ventilation ,Anakinra ,Multidisciplinary ,business.industry ,COVID-19 ,Biological Sciences ,medicine.disease ,Pneumonia ,030104 developmental biology ,Respiratory failure ,medicine.symptom ,Cytokine storm ,business ,030217 neurology & neurosurgery ,interleukin-1 ,medicine.drug ,anakinra - Abstract
Around the tenth day after diagnosis, ∼20% of patients with coronavirus disease 2019 (COVID-19)−associated pneumonia evolve toward severe oxygen dependence (stage 2b) and acute respiratory distress syndrome (stage 3) associated with systemic inflammation often termed a “cytokine storm.” Because interleukin-1 (IL-1) blocks the production of IL-6 and other proinflammatory cytokines, we treated COVID-19 patients early in the disease with the IL-1 receptor antagonist, anakinra. We retrospectively compared 22 patients from three different centers in France with stages 2b and 3 COVID-19−associated pneumonia presenting with acute severe respiratory failure and systemic inflammation who received either standard-of-care treatment alone (10 patients) or combined with intravenous anakinra (12 patients). Treatment started at 300 mg⋅d −1 for 5 d, then tapered with lower dosing over 3 d. Both populations were comparable for age, comorbidities, clinical stage, and elevated biomarkers of systemic inflammation. All of the patients treated with anakinra improved clinically ( P < 0.01), with no deaths, significant decreases in oxygen requirements ( P < 0.05), and more days without invasive mechanical ventilation ( P < 0.06), compared with the control group. The effect of anakinra was rapid, as judged by significant decrease of fever and C-reactive protein at day 3. A mean total dose of 1,950 mg was infused with no adverse side effects or bacterial infection. We conclude that early blockade of the IL-1 receptor is therapeutic in acute hyperinflammatory respiratory failure in COVID-19 patients. more...
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- 2020
166. Estimation de la survie globale à partir des données administratives hospitalières avec intégration des données de décès de l'INSEE : l'expérience du Centre Oscar Lambret
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M. Barthoulot, A. Hammoudi, F. Craynest, E. Aymes, M. Cucchi, D. Cauchois, E. Lartigau, and M. Le deley
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Epidemiology ,Public Health, Environmental and Occupational Health - Published
- 2022
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167. Retour d'expérience sur le projet de chaînage entre les données hospitalières du Centre Oscar Lambret et les fichiers de décès de l'Insee
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M. Barthoulot, F. Craynest, A. Hammoudi, E. Aymes, D. Cauchois, M. Cucchi, M. Le Deley, and E. Lartigau
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Epidemiology ,Public Health, Environmental and Occupational Health - Published
- 2022
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168. P.195 Quantitative skeletal muscle MRI of golden retriever muscular dystrophy dogs
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B. Marty, I. Barthélémy, X. Cauchois, P. Baudin, Y. Fromes, S. Blot, and H. Reyngoudt
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Neurology ,Pediatrics, Perinatology and Child Health ,Neurology (clinical) ,Genetics (clinical) - Published
- 2022
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169. Anatomie faciale et toxine botulique : les balances musculaires
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Trevidic, P., Cauchois, R., and Ingallina, F.
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- 2009
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170. Interprétation et mémorisation de l’information pour parotidectomie
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Laccourreye, L., Werner, A., Cauchois, R., and Laccourreye, O.
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- 2009
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171. "True" Antiphospholipid Syndrome in COVID-19: Contribution of the Follow-up of Antiphospholipid Autoantibodies.
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Arcani, Robin, Cauchois, Raphaël, Suchon, Pierre, Weber, Samuel, Jean, Rodolphe, Jarrot, Pierre-André, Rey, Louise, Venton, Geoffroy, Koubi, Marie, Muller, Romain, Bertin, Daniel, Mège, Jean-Louis, Kaplanski, Gilles, and Bardin, Nathalie more...
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ANTIPHOSPHOLIPID syndrome , *AUTOANTIBODIES , *COVID-19 - Abstract
Clinical data were compared between 66 aPLs patients (59.1% with LA, 33.3% with aCL IgG, 9.1% with aCL IgM, 7.6% with aB2GPI IgG, and 15.2% with aB2GPI IgM) and 119 patients without aPLs. We found that persistent aPLs were associated with thrombotic events as defined for the APS diagnosis [7] in three patients from the time of hospitalization for COVID-19 to the end of the follow-up, that is, 1.6% of patients corresponded to a "true" APS. There were 53 patients (28.6%) with only one aPL; 10 patients (5.4%) had two aPLs, and three patients (1.6%) were triple-aPL positive. There were 36 of 167 eligible patients (21.6%) transferred to the ICU, and 7 patients out of 167 eligible patients (4.2%) required mechanical ventilation; 58 patients (31.4%) needed supplemental oxygen flow greater than 6 L/minute. [Extracted from the article] more...
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- 2023
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172. Combined Anakinra and Ruxolitinib treatment to rescue extremely ill COVID-19 patients: A pilot study.
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Kaplanski, Gilles, Bontemps, Denis, Esnault, Pierre, Blasco, Valery, Carvelli, Julien, Delarbre, David, Cauchois, Raphael, Forel, Jean-Marie, and Papazian, Laurent
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- 2021
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173. L’endothélium au cours du PTT : faites entrer l’accusé !
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Cauchois, Raphaël, primary and Poullin, Pascale, additional
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- 2021
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174. Generalized Distinguishing Attack: A New Cryptanalysis of AES-like Permutations.
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Victor Cauchois, Clément Gomez, and Reynald Lercier
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- 2017
175. Syndrome catastrophique des antiphospholipides traité par éculizumab : à propos de deux cas
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Karin Mazodier, Gilles Kaplanski, A. Le Roux, Rodolphe Jean, V. Roumieu, and R. Cauchois
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Gastroenterology ,Internal Medicine - Published
- 2021
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176. Effects of Cell Confluence on the Immunological and Migration Receptors of Wharton Jelly’s Mesenchymal Stem Cells
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Charlotte Voisin, Ghislaine Cauchois, Danièle Bensoussan, and Céline Huselstein
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Confluency ,Wharton's jelly ,Mesenchymal stem cell ,Biology ,Receptor ,Cell biology - Abstract
Mesenchymal stem/stromal cells (MSCs) are a promising tool for cell-based therapy thanks to their ability to secrete trophic factors and immunomodulatory potential. So far, these cells are used to treat a variety of inflammatory diseases like sepsis or severe graft-versus-host disease (GvHD). Considering the number of cells required for their use in cell therapy (between 1–3 × 106 cells/kg), a primary expansion is necessary. However, after an intravenous injection, few cells are found in tissue lesion or in bone marrow. One hypothesis is that chemotactic signals that guide MSCs to an appropriate environment would be altered during in vitro expansion. It is therefore essential to define better conditions of expansion, particularly with good manufacturing process, to obtain enough MSCs, while preserving their immunomodulatory properties and expression of migration molecules. The aim of this research project is to investigate the effect of cell confluence on migration potential and immunological properties of Wharton’s jelly (WJ) MSCs. This research is investigated with 3 levels confluency, at 50, 80 and 100% of confluence by using good manufacturing practice (GMP). We found a better expression of immunomodulatory molecules (PD-L1) and migratory molecules (CD44, membrane CXCR7) for cells that reached 50% confluence. Furthermore, co-stimulatory molecules appear to be less expressed when MSCs are at low confluency. In conclusion low confluence culture should be privileged to improve immunomodulatory and migration phenotype. more...
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- 2021
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177. Successful heart transplantation for COVID-19-associated post-infectious fulminant myocarditis
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Gaudriot, Baptiste, Mansour, Alexandre, Thibault, Vincent, Lederlin, Mathieu, Cauchois, Aurélie, Lelong, Bernard, Ross, James T, Leurent, Guillaume, Tadié, Jean-Marc, Revest, Matthieu, Verhoye, Jean-Philippe, Flecher, Erwan, Nesseler, Nicolas, CHU Pontchaillou [Rennes], University of California [San Francisco] (UCSF), University of California, Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), University of California [San Francisco] (UC San Francisco), University of California (UC), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM) more...
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Extracorporeal membrane oxygenation ,COVID‐19 ,RC666-701 ,COVID-19 ,Diseases of the circulatory (Cardiovascular) system ,Case Report ,[SDV.IB]Life Sciences [q-bio]/Bioengineering ,Fulminant myocarditis ,Heart transplantation ,Cardiogenic shock - Abstract
International audience; Various clinical presentations of the 2019 coronavirus disease (COVID-19) have been described, including post-infectious acute and fulminant myocarditis. Here, we describe the case of a young patient admitted for COVID-19-associated post-infectious fulminant myocarditis. Despite optimal pharmacologic management, haemodynamic status worsened requiring support by veno-arterial extracorporeal membrane oxygenation. Emergent heart transplantation was required at Day 11 given the absence of cardiac function improvement. The diagnosis of post-infectious COVID-19-associated myocarditis was made from both pathologic examination of the explanted heart and positive SARS-CoV-2 serology. more...
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- 2021
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178. Effect of anakinra on mortality in COVID-19: a patient level meta-analysis
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Thomas Huet, Evangelos J. Giamarellos-Bourboulis, Jesper Eugen-Olsen, Gilles Hayem, Raphael Cauchois, Gilles Chatellier, Gilles Kaplanski, Mihai G. Netea, Evdoxia Kyriazopoulou, Mario Clerici, Alessandra Bandera, Francisco Veas, Giulio Cavalli, Lorenzo Dagna, Davide Mangioni, Andrea Gori, Emma J. Kooistra, Peter Pickkers, Miltiades Kyprianou, Matthijs Kox, Hélène Beaussier, Jos W. M. van der Meer, Marco Gattorno, and Emanuele Pontali more...
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Anakinra ,medicine.medical_specialty ,business.industry ,Secondary infection ,Odds ratio ,Placebo ,law.invention ,Randomized controlled trial ,law ,Meta-analysis ,Internal medicine ,medicine ,Clinical endpoint ,Adverse effect ,business ,medicine.drug - Abstract
SummaryBackgroundAnakinra may represent an important therapy to improve the prognosis of COVID-19 patients. This meta-analysis using individual patient data was designed to assess the efficacy and safety of anakinra treatment in patients with COVID-19.MethodsBased on a pre-specified protocol (PROSPERO: CRD42020221491), a systematic literature search was performed in MEDLINE (PubMed), Cochrane, medRxiv.org, bioRxiv.org and clinicaltrials.gov databases for trials in COVID-19 comparing administration of anakinra with standard-of-care and/or placebo. Individual patient data from eligible trials were requested. The primary endpoint was the mortality rate and the secondary endpoint was safety.FindingsLiterature search yielded 209 articles, of which 178 articles fulfilled screening criteria and were full-text assessed. Aggregate data on 1185 patients from 9 studies were analyzed and individual patient data on 895 patients from 6 studies were collected. Most studies used historical controls. Mortality was significantly lower in anakinra-treated patients (38/342 [11·1%]) as compared with 137/553 (24·8%) observed in patients receiving standard-of-care and/or placebo on top of standard-of-care (137/553 [24·8%]); adjusted odds ratio (OR), 0·32; 95% CI, 0·20 to 0·51; p 100 mg/L (OR 0·28,95%CI 0·27-1·47). Safety issues, such as increase of secondary infections, did not emerge.InterpretationAnakinra may be a safe anti-inflammatory treatment option in patients hospitalized with moderate-to-severe COVID-19 pneumonia to reduce mortality, especially in the presence of hyperinflammation signs such as CRP>100mg /L.FundingSobi.Research in contextEvidence before this studySince the emergence of the COVID-19 pandemic, numerous drugs have been tried in an effort to prevent major detrimental consequences, such as respiratory and multiorgan failure and death. Early during the pandemic, it was realized that drugs aiming to regulate the immune host reaction may play an important role in the treatment of COVID-19. Evidence from a small number of patients with moderate or severe COVID-19 treated with anakinra, and interleukin-1 receptor antagonist, has suggested therapeutic efficacy. We systematically searched all available literature and aimed to present cumulative evidence of anakinra treatment in COVID-19 and the related effect on mortality.Added value of this studyThis is the first patient-level analysis on the effect of anakinra treatment in COVID-19 patients, which, on the one hand, suggests a significant benefit in the reduction of mortality and on the other hand, reassures safety of the treatment. Most importantly, the current study identifies a subgroup of patients with CRP>100mg/L, that may benefit most from treatment with anakinra. Confirmation of these effects in larger randomized clinical trials (RCTs) is urgently needed.Implications of all the available evidenceAnakinra may be an effective and safe immunomodulatory treatment in moderate-to-severe cases of pneumonia due to COVID-19 to prevent unfavorable outcomes. Anakinra may be helpful to avoid adverse events, such as breakthrough infections observed often with dexamethasone use, and may be considered an alternative in specific subgroups of patients e.g. diabetics. Larger trials, summarized in the Table, are ongoing and their results are urgently needed to investigate anakinra’s best place in the treatment of COVID-19. more...
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- 2021
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179. Excess body weight is an independent risk factor for severe forms of COVID-19
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Noémie Dubois, Roch Giorgi, Sophie Béliard, Gilles Kaplanski, Raphael Cauchois, Manon Lojou, Marie Koubi, René Valéro, Rachel Grangeot, Audrey Bégu, Léa Pietri, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale (SESSTIM - U1252 INSERM - Aix Marseille Univ - UMR 259 IRD), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biostatistique et technologies de l'information et de la communication (BioSTIC) - [Hôpital de la Timone - APHM] (BiosTIC ), Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), and Malbec, Odile more...
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Male ,0301 basic medicine ,Multivariate analysis ,Endocrinology, Diabetes and Metabolism ,[SDV]Life Sciences [q-bio] ,Comorbidity ,Disease ,Overweight ,Severity of Illness Index ,Body Mass Index ,law.invention ,0302 clinical medicine ,Endocrinology ,Risk Factors ,law ,Aged, 80 and over ,Middle Aged ,Prognosis ,Intensive care unit ,3. Good health ,Hospitalization ,[SDV] Life Sciences [q-bio] ,Female ,France ,medicine.symptom ,medicine.medical_specialty ,Critical Illness ,030209 endocrinology & metabolism ,Excess body weight ,Article ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,Obesity ,Risk factor ,Obesity comorbidities ,Aged ,Retrospective Studies ,business.industry ,SARS-CoV-2 ,Body Weight ,COVID-19 ,Odds ratio ,Anthropometry ,medicine.disease ,030104 developmental biology ,business - Abstract
Background and aims Few studies distinguished the independent role of overweight/obesity or their associated-comorbidities in the evolution towards severe forms of COVID-19. Obesity as a unifying risk factor for severe COVID-19 is an emerging hypothesis. The aim of this study was to evaluate whether excessive body weight per se, was a risk factor for developing a severe form of COVID-19. Patients and methods We included 131 patients hospitalized for COVID-19 pneumonia in a single center of the internal medicine department in Marseille, France. We recorded anthropometric and metabolic parameters such as fasting glycaemia, insulinemia, HOMA-IR, lipids, and all clinical criteria linked to SARS-CoV-2 infection at the admission. Excess body weight was defined by a BMI ≥ 25 kg/m2. The occurrence of a serious event was defined as a high-debit oxygen requirement over 6 L/min, admission into the intensive care unit, or death. Results Among 113 patients, two thirds (n = 76, 67%) had an excess body weight. The number of serious events was significantly higher in excess body weight patients compared to normal weight patients (respectively 25% vs 8%, p = 0.03) although excess body weight patients were younger (respectively 63.6 vs 70.3 years old, p = 0.01). In multivariate analyses, the excess body weight status was the only predictor for developing a serious event linked to SARS-CoV-2 infection, with an odds ratio at 5.6 (95% CI: 1.30–23.96; p = 0.02), independently of previous obesity associated comorbidities. There was a trend towards a positive association between the BMI (normal weight, overweight and obesity) and the risk of serious events linked to COVID-19, with a marked increase from 8.1% to 20% and 30.6% respectively (p = 0.05). Conclusion Excess body weight was significantly associated with severe forms of the disease, independently of its classical associated comorbidities. Physicians and specialists in Public Health must be sensitized to better protect people with an excess body weight against SARS-CoV-2 infection., Highlights • Excess BW multiply by 5.6 the risk of serious event linked to COVID-19 • Excess BW is linked to severe forms of COVID-19 independently of obesity comorbidities. • Scientific community must be sensitized to better protect people with an excess BW. more...
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- 2021
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180. An Improved In-line Inkjet Printing Process for 3D Multilayer Passive Devices
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Yakoub, A., Saadaoui, M., Cauchois, R., Li, J-M., and Benaben, P.
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- 2012
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181. Impact of variable frequency microwave and rapid thermal sintering on microstructure of inkjet-printed silver nanoparticles
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Cauchois, Romain, Saadaoui, Mohamed, Yakoub, Abdelwahhab, Inal, Karim, Dubois-Bonvalot, Beatrice, and Fidalgo, Jean-Christophe
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- 2012
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182. Apports et limites de la fiche écrite lors de l’information sur les risques encourus avant un acte chirurgical programmé
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Laccourreye, Laurent, Werner, Alfred, Cauchois, Régis, and Laccourreye, Ollivier
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- 2008
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183. Analyse des facteurs qui influent sur la mémorisation et l’interprétation par le malade de l’information concernant les risques de la thyroïdectomie
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Laccourreye, Ollivier, Cauchois, Régis, and Werner, Alfred
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- 2008
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184. L’endothélium au cours du PTT : faites entrer l’accusé !
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Pascale Poullin and Raphaël Cauchois
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Biochemistry (medical) ,Clinical Biochemistry ,Hematology - Published
- 2021
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185. In-hospital emergencies versus out-of-hospital emergencies admitted to ICU: is the outcome different? An observational study
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Charlotte Allez, Audrey Le Saux, Vanessa Pauly, Emi Cauchois, Fouad Bouzana, Marc Gainnier, Antoine Tilmont, Jeremy Bourenne, and Julien Carvelli
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Out of hospital ,medicine.medical_specialty ,business.industry ,Emergency medicine ,medicine ,Observational study ,business ,Outcome (game theory) - Abstract
Background: Rapid Response Systems (RRS) are now commonly implemented throughout hospital health systems to manage in-hospital emergencies (IHE). There is limited data on characteristics and outcomes of such patients admitted to an intensive care unit (ICU). The goal was to determine whether the hospital mortality of ICU patients was different depending on their admission pathway: in-hospital via rapid response teams (RRT), or out-of-hospital emergencies (OHE) via prehospital emergency medical systems. Results: Out of 422 ICU admissions (Timone University Hospital ICU), 241 patients were retrospectively (2019-2020) included: 74 IHE versus 167 OHE. In-hospital mortality rates did not differ between both cohorts (n = 31(42%) vs. 63(39%) respectively, NS). IHE patients were older and had more comorbidities (immunosuppression and ongoing malignancy). OHE patients had more severe organ failures at presentation with more frequent mechanical ventilation support. Independent global hospital mortality risk factors were ongoing malignancy (OR = 10.4 [2.7-40], p < 0.001), SAPS II (OR = 1.05 [1.03-1.08], p < 0.0001) and SOFA scores (OR = 1.14 [1.01-1.3], p < 0.05), hemorrhagic stroke as admission diagnosis (OR = 8.4 [2.7-26], p < 0.001), and arterial lactate on arrival (OR = 1.11 [1.03-1.2], p < 0.01). Conclusion: This study provides a thorough and comprehensive analysis of characteristics and outcomes of ICU admissions following a mature rapid response activation system, compared to the “conventional” out-of-hospital admission pathway. Despite the more vulnerable background of IHE patients, hospital mortality does not differ, supporting the use of early RRS to identify deteriorating ward patients. Take-home message: Hospital mortality does not differ between in-hospital emergencies admitted to intensive care unit and conventional out-of-hospital admissions, supporting the use of early rapid response systems and the importance of early intensive care unit admission. more...
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- 2021
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186. Response of a CMS HGCAL silicon-pad electromagnetic calorimeter prototype to 20-300 GeV positrons
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B. Acar, G. Adamov, C. Adloff, S. Afanasiev, N. Akchurin, B. Akgün, F.A. Khan, M. Alhusseini, J. Alison, A. Alpana, G. Altopp, M. Alyari, S. An, S. Anagul, I. Andreev, P. Aspell, I.O. Atakisi, O. Bach, A. Baden, G. Bakas, A. Bakshi, S. Bannerjee, P. Bargassa, D. Barney, F. Beaudette, F. Beaujean, E. Becheva, A. Becker, P. Behera, A. Belloni, T. Bergauer, M. Besancon, S. Bhattacharya, D. Bhowmik, B. Bilki, P. Bloch, A. Bodek, M. Bonanomi, A. Bonnemaison, S. Bonomally, J. Borg, F. Bouyjou, N. Bower, D. Braga, J. Brashear, E. Brondolin, P. Bryant, A. Buchot Perraguin, J. Bueghly, B. Burkle, A. Butler-Nalin, O. Bychkova, S. Callier, D. Calvet, X. Cao, A. Cappati, B. Caraway, S. Caregari, A. Cauchois, L. Ceard, Y.C. Cekmecelioglu, S. Cerci, G. Cerminara, M. Chadeeva, N. Charitonidis, R. Chatterjee, Y.M. Chen, Z. Chen, H.J. Cheng, K.y. Cheng, S. Chernichenko, H. Cheung, C.H. Chien, S. Choudhury, D. Čoko, G. Collura, F. Couderc, M. Danilov, D. Dannheim, W. Daoud, P. Dauncey, A. David, G. Davies, O. Davignon, E. Day, P. DeBarbaro, F. De Guio, C. de La Taille, M. De Silva, P. Debbins, M.M. Defranchis, E. Delagnes, J.M. Deltoro Berrio, G. Derylo, P.G. Dias de Almeida, D. Diaz, P. Dinaucourt, J. Dittmann, M. Dragicevic, S. Dugad, F. Dulucq, I. Dumanoglu, V. Dutta, S. Dutta, M. Dünser, J. Eckdahl, T.K. Edberg, M. El Berni, F. Elias, S.C. Eno, Y. Ershov, P. Everaerts, S. Extier, F. Fahim, C. Fallon, G. Fedi, B.A. Fontana Santos Alves, E. Frahm, G. Franzoni, J. Freeman, T. French, P. Gandhi, S. Ganjour, X. Gao, A. Garcia-Bellido, F. Gastaldi, Z. Gecse, Y. Geerebaert, H. Gerwig, O. Gevin, S. Ghosh, A. Gilbert, W. Gilbert, K. Gill, C. Gingu, S. Gninenko, A. Golunov, I. Golutvin, T. Gonzalez, N. Gorbounov, L. Gouskos, A.B. Gray, Y. Gu, F. Guilloux, Y. Guler, E. Gülmez, J. Guo, E. Gurpinar Guler, M. Hammer, H.M. Hassanshahi, K. Hatakeyama, A. Heering, V. Hegde, U. Heintz, N. Hinton, J. Hirschauer, J. Hoff, W.-S. Hou, X. Hou, H. Hua, J. Incandela, A. Irshad, C. Isik, S. Jain, H.R. Jheng, U. Joshi, V. Kachanov, A. Kalinin, L. Kalipoliti, A. Kaminskiy, A. Kapoor, O. Kara, A. Karneyeu, M. Kaya, O. Kaya, A. Kayis Topaksu, A. Khukhunaishvili, J. Kiesler, M. Kilpatrick, S. Kim, K. Koetz, T. Kolberg, O.K. Köseyan, A. Kristić, M. Krohn, K. Krüger, N. Kulagin, S. Kulis, S. Kunori, C.M. Kuo, V. Kuryatkov, S. Kyre, Y. Lai, K. Lamichhane, G. Landsberg, C. Lange, J. Langford, M.Y. Lee, A. Levin, A. Li, B. Li, J.H. Li, Y.Y. Li, H. Liao, D. Lincoln, L. Linssen, R. Lipton, Y. Liu, A. Lobanov, R.-S. Lu, M. Lupi, I. Lysova, A.-M. Magnan, F. Magniette, A. Mahjoub, A.A. Maier, A. Malakhov, S. Mallios, I. Mandjavize, M. Mannelli, J. Mans, A. Marchioro, A. Martelli, G. Martinez, P. Masterson, B. Meng, T. Mengke, A. Mestvirishvili, I. Mirza, S. Moccia, G.B. Mohanty, F. Monti, I. Morrissey, S. Murthy, J. Musić, Y. Musienko, S. Nabili, A. Nagar, M. Nguyen, A. Nikitenko, D. Noonan, M. Noy, K. Nurdan, C. Ochando, B. Odegard, N. Odell, H. Okawa, Y. Onel, W. Ortez, J. Ozegović, S. Ozkorucuklu, E. Paganis, D. Pagenkopf, V. Palladino, S. Pandey, F. Pantaleo, C. Papageorgakis, I. Papakrivopoulos, J. Parshook, N. Pastika, M. Paulini, P. Paulitsch, T. Peltola, R. Pereira Gomes, H. Perkins, P. Petiot, T. Pierre-Emile, F. Pitters, E. Popova, H. Prosper, M. Prvan, I. Puljak, H. Qu, T. Quast, R. Quinn, M. Quinnan, M.T. Ramos Garcia, K.K. Rao, K. Rapacz, L. Raux, G. Reichenbach, M. Reinecke, M. Revering, A. Roberts, T. Romanteau, A. Rose, M. Rovere, A. Roy, P. Rubinov, R. Rusack, V. Rusinov, V. Ryjov, O.M. Sahin, R. Salerno, A.M. Sanchez Rodriguez, R. Saradhy, T. Sarkar, M.A. Sarkisla, J.B. Sauvan, I. Schmidt, M. Schmitt, E. Scott, C. Seez, F. Sefkow, S. Sharma, I. Shein, A. Shenai, R. Shukla, E. Sicking, P. Sieberer, P. Silva, A.E. Simsek, Y. Sirois, V. Smirnov, U. Sozbilir, E. Spencer, A. Steen, J. Strait, N. Strobbe, J.W. Su, E. Sukhov, L. Sun, D. Sunar Cerci, C. Syal, B. Tali, C.L. Tan, J. Tao, I. Tastan, T. Tatli, R. Thaus, S. Tekten, D. Thienpont, E. Tiras, M. Titov, D. Tlisov, U.G. Tok, J. Troska, L.-S. Tsai, Z. Tsamalaidze, G. Tsipolitis, A. Tsirou, N. Tyurin, S. Undleeb, D. Urbanski, V. Ustinov, A. Uzunian, M. Van de Klundert, J. Varela, M. Velasco, O. Viazlo, M.V. Barreto Pinto, P. Vichoudis, T. Virdee, R. Vizinho de Oliveira, J. Voelker, E. Voirin, M. Vojinovic, A. Wade, C. Wang, F. Wang, X. Wang, Z. Wang, M. Wayne, S.N. Webb, A. Whitbeck, D. White, R. Wickwire, J.S. Wilson, D. Winter, H.y. Wu, L. Wu, M. Wulansatiti Nursanto, C.H. Yeh, R. Yohay, D. Yu, G.B. Yu, S.S. Yu, C. Yuan, F. Yumiceva, I. Yusuff, A. Zacharopoulou, N. Zamiatin, A. Zarubin, S. Zenz, A. Zghiche, H. Zhang, J. Zhang, Y. Zhang, Z. Zhang, Laboratoire Leprince-Ringuet (LLR), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherches sur les lois Fondamentales de l'Univers (IRFU), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Organisation de Micro-Électronique Générale Avancée (OMEGA), and CMS HGCAL more...
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Physics - Instrumentation and Detectors ,Physics::Instrumentation and Detectors ,electromagnetic [calorimeter] ,energy resolution ,FOS: Physical sciences ,High Energy Physics - Experiment ,design [detector] ,High Energy Physics - Experiment (hep-ex) ,Calorimeters ,[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex] ,[PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det] ,ddc:610 ,Detectors and Experimental Techniques ,Instrumentation ,physics.ins-det ,spatial resolution ,Mathematical Physics ,detector: design ,irradiation ,Large detector systems for particle and astroparticle physics ,hep-ex ,CMS ,Instrumentation and Detectors (physics.ins-det) ,Si microstrip and pad detectors ,calorimeter: electromagnetic ,angular resolution ,electronics: readout ,Physics::Accelerator Physics ,semiconductor detector ,High Energy Physics::Experiment ,readout [electronics] ,Particle Physics - Experiment ,performance - Abstract
Journal of Instrumentation 17(05), P05022 (2022). doi:10.1088/1748-0221/17/05/P05022, The Compact Muon Solenoid collaboration is designing a new high-granularity endcap calorimeter, HGCAL, to be installed later this decade. As part of this development work, a prototype system was built, with an electromagnetic section consisting of 14 double-sided structures, providing 28 sampling layers. Each sampling layer has an hexagonal module, where a multipad large-area silicon sensor is glued between an electronics circuit board and a metal baseplate. The sensor pads of approximately 1.1 cm$^{2}$ are wire-bonded to the circuit board and are readout by custom integrated circuits. The prototype was extensively tested with beams at CERN's Super Proton Synchrotron in 2018. Based on the data collected with beams of positrons, with energies ranging from 20 to 300 GeV, measurements of the energy resolution and linearity, the position and angular resolutions, and the shower shapes are presented and compared to a detailed Geant4 simulation., Published by Inst. of Physics, London more...
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- 2021
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187. DIFFERENTIAL PRIVACY FOR ENERGY DATA PUBLICATION
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G. Agoua, P. Cauchois, O. Chaouy, I. Gazeau, and B. Grossin
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- 2021
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188. Axial articular manifestations in primary Sjögren‘s syndrome: Association with spondyloarthritis
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Gilles Kaplanski, Jean Roudier, Raphael Cauchois, Rodolphe Jean, Olivier Darmon, Karin Mazodier, Pierre-André Jarrot, Nathalie Balandraud, Robin Arcani, Arthrites autoimmunes (AA), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM) more...
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Adult ,Male ,musculoskeletal diseases ,0301 basic medicine ,medicine.medical_specialty ,Adolescent ,[SDV]Life Sciences [q-bio] ,Immunology ,Arthritis ,primary Sjogren syndrome ,Young Adult ,03 medical and health sciences ,Psoriatic arthritis ,0302 clinical medicine ,Rheumatology ,Internal medicine ,Spondylarthritis ,medicine ,Humans ,Immunology and Allergy ,Spondylitis, Ankylosing ,In patient ,Sacroiliitis ,Primary Sjögren Syndrome ,HLA-B27 Antigen ,ComputingMilieux_MISCELLANEOUS ,Aged ,030203 arthritis & rheumatology ,Ankylosing spondylitis ,business.industry ,Middle Aged ,spondyloarthritis ,medicine.disease ,3. Good health ,stomatognathic diseases ,Sjogren's Syndrome ,030104 developmental biology ,axial articular manifestation ,biotherapy ,Female ,business ,Rheumatism - Abstract
Objective.To assess the prevalence of axial articular manifestations (AAMs) in patients with primary Sjögren syndrome (pSS), to investigate whether these symptoms reveal an associated spondyloarthritis (SpA), and to assess their therapeutic management.Methods.Among 148 consecutive patients with pSS fulfilling European League Against Rheumatism (EULAR)/American College of Rheumatology 2019 classification criteria followed between 2010 and 2018, we selected those who presented with AAMs. The association with SpA was retained when patients fulfilled Assessment of SpA international Society criteria.Results.A total of 29 patients (20%, 28 women) with a median age of 43 years (range 15–65 yrs), were identified. The main extraglandular features were peripheral arthralgia and arthritis in 93% and 90% of patients, respectively. Positive anti-Ro/SSA (anti-SSA) antibody was reported in 62%. AAMs were inaugural in 7%, delayed from the diagnostic of pSS in 7%, and occurred concomitantly in 86% of patients. AAMs were not associated to multisystemic involvement of pSS. Radiographic sacroiliitis was mentioned in 65%, and HLA-B27 was positive in 13%. The diagnosis of SpA was retained in 23/29 patients (79%), among which 74% and 26% fulfilled psoriatic arthritis and ankylosing spondylitis criteria, respectively. There was no phenotypic difference according to the anti-SSA antibody status. With a median follow-up of 60 months (range: 5–96), 61% of patients with associated SpA required biotherapies, mainly of anti–tumor necrosis factor-α or anti–interleukin 17A molecules with a good clinical outcome in 64% and no effect on pSS.Conclusion.AAMs are not uncommon in patients with pSS and may reveal an associated SpA. Treatment of AAMs, especially when clearly associated with SpA, may necessitate biologics, following SpA-management therapeutic guidelines. more...
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- 2021
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189. Effect of anakinra on mortality in patients with COVID-19: a systematic review and patient-level meta-analysis
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Kyriazopoulou, E. Huet, T. Cavalli, G. Gori, A. Kyprianou, M. Pickkers, P. Eugen-Olsen, J. Clerici, M. Veas, F. Chatellier, G. Kaplanski, G. Netea, M.G. Pontali, E. Gattorno, M. Cauchois, R. Kooistra, E. Kox, M. Bandera, A. Beaussier, H. Mangioni, D. Dagna, L. van der Meer, J.W.M. Giamarellos-Bourboulis, E.J. Hayem, G. Netea, M.G. van der Meer, J.W.M. Giamarellos-Bourboulis, E.J. Volpi, S. Sormani, M.P. Signori, A. Bozzi, G. Minoia, F. Aliberti, S. Grasselli, G. Alagna, L. Lombardi, A. Ungaro, R. Agostoni, C. Blasi, F. Costantino, G. Fracanzani, A.L. Montano, N. Peyvandi, F. Sottocorno, M. Muscatello, A. Filocamo, G. Papadopoulos, A. Mouktaroudi, M. Karakike, E. Saridaki, M. Gkavogianni, T. Katrini, K. Vechlidis, N. Avgoustou, C. Chalvatzis, S. Marantos, T. Damoulari, C. Damoraki, G. Ktena, S. Tsilika, M. Koufargyris, P. Karageorgos, A. Droggiti, D.-I. Koliakou, A. Poulakou, G. Tsiakos, K. Myrodia, D.-M. Gravvani, A. Trontzas, I.P. Syrigos, K. Kalomenidis, I. Kranidioti, E. Panagopoulos, P. Petrakis, V. Metallidis, S. Loli, G. Tsachouridou, O. Dalekos, G.N. Gatselis, N. Stefos, A. Georgiadou, S. Lygoura, V. Milionis, H. Kosmidou, M. Papanikolaou, I.C. Akinosoglou, K. Giannitsioti, E. Chrysos, G. Mavroudis, P. Sidiropoulou, C. Adamis, G. Fragkou, A. Rapti, A. Alexiou, Z. Symbardi, S. Masgala, A. Kostaki, K. Kostis, E. Samarkos, M. Bakakos, P. Tzavara, V. Dimakou, K. Tzatzagou, G. Chini, M. Kotsis, V. Tsoukalas, G. Bliziotis, I. Doumas, M. Argyraki, A. Kainis, I. Fantoni, M. Cingolani, A. Angheben, A. Cardellino, C.S. Castelli, F. Serino, F.S. Nicastri, E. Ippolito, G. Bassetti, M. Selmi, C. International Collaborative Group for Anakinra in COVID-19 more...
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Background: Anakinra might improve the prognosis of patients with moderate to severe COVID-19 (ie, patients requiring oxygen supplementation but not yet receiving organ support). We aimed to assess the effect of anakinra treatment on mortality in patients admitted to hospital with COVID-19. Methods: For this systematic review and individual patient-level meta-analysis, a systematic literature search was done on Dec 28, 2020, in Medline (PubMed), Cochrane, medRxiv, bioRxiv, and the ClinicalTrials.gov databases for randomised trials, comparative studies, and observational studies of patients admitted to hospital with COVID-19, comparing administration of anakinra with standard of care, or placebo, or both. The search was repeated on Jan 22, 2021. Individual patient-level data were requested from investigators and corresponding authors of eligible studies; if individual patient-level data were not available, published data were extracted from the original reports. The primary endpoint was mortality after 28 days and the secondary endpoint was safety (eg, the risk of secondary infections). This study is registered on PROSPERO (CRD42020221491). Findings: 209 articles were identified, of which 178 full-text articles fulfilled screening criteria and were assessed. Aggregate data on 1185 patients from nine studies were analysed, and individual patient-level data on 895 patients were provided from six of these studies. Eight studies were observational and one was a randomised controlled trial. Most studies used historical controls. In the individual patient-level meta-analysis, after adjusting for age, comorbidities, baseline ratio of the arterial partial oxygen pressure divided by the fraction of inspired oxygen (PaO2/FiO2), C-reactive protein (CRP) concentrations, and lymphopenia, mortality was significantly lower in patients treated with anakinra (38 [11%] of 342) than in those receiving standard of care with or without placebo (137 [25%] of 553; adjusted odds ratio [OR] 0·32 [95% CI 0·20–0·51]). The mortality benefit was similar across subgroups regardless of comorbidities (ie, diabetes), ferritin concentrations, or the baseline PaO2/FiO2. In a subgroup analysis, anakinra was more effective in lowering mortality in patients with CRP concentrations higher than 100 mg/L (OR 0·28 [95% CI 0·17–0·47]). Anakinra showed a significant survival benefit when given without dexamethasone (OR 0·23 [95% CI 0·12–0·43]), but not with dexamethasone co-administration (0·72 [95% CI 0·37–1·41]). Anakinra was not associated with a significantly increased risk of secondary infections when compared with standard of care (OR 1·35 [95% CI 0·59–3·10]). Interpretation: Anakinra could be a safe, anti-inflammatory treatment option to reduce the mortality risk in patients admitted to hospital with moderate to severe COVID-19 pneumonia, especially in the presence of signs of hyperinflammation such as CRP concentrations higher than 100 mg/L. Funding: Sobi. © 2021 Elsevier Ltd more...
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- 2021
190. CO2 Footprint of the Norwegian Longship Project
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Torild R. Nissen-lie, Mike Carpenter, Laila Iren Helgesen, Audun Røsjorde, Gaelle Cauchois, and Magne Prestholdt
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Waste-to-energy ,Footprint ,Waste management ,Waste heat ,Greenhouse gas ,Carbon capture and storage (timeline) ,Environmental science ,Tonne ,Storage efficiency ,Nuclear decommissioning - Abstract
An analysis of the CO storage efficiency of the Longship project has been performed using a spreadsheet based LCA tool. Results are presented in terms of the CO2 footprint for each value chain measured in units of “tonnes of CO2 equivalent emissions per tonne of CO2 stored underground”. In other words, the results from the tool show the impact of the implementation and operation of the chain on the CO2 storage efficiency. The work was commissioned by Gassnova, the Norwegian State Enterprise for Carbon Capture and Storage. The work was performed by DNV GL and Carbon Limits The method used is consistent with the principles of ISO 14040 “Life Cycle Analysis – principles and framework” and ISO 14044 “Life Cycle Analysis – requirements and guidelines” and has been designed to calculate CO2 equivalent emissions to atmosphere in a 100-year perspective. The functional unit of the system studied is 1 tonne of CO2 stored, where storage refers to permanent geological storage .as defined in ISO 27914 “Carbon dioxide capture, transportation and geological storage – Geological storage”. The tool is capable of accounting for direct and indirect greenhouse gas emissions of each building block of the Carbon Capture and Storage (CCS) chain for the life cycle of the project. As such, for each building block, all project phases are included (construction, operation, decommissioning for capture and transport – construction, injection, post injection, decommissioning and post closure for storage) and for each phase CO2 emissions from the use of fuel, energy, chemicals, materials and transport are considered. In the Longship project, CO2 is captured from two capture sites, the cement factory of Norcem in Brevik and the waste to energy facility at Klemetsrud in Oslo, transported by ship to a receiving terminal at Naturgassparken near Bergen and further transported in a subsea pipeline to the Aurora CO2 storage license area near the Troll field in the North Sea for final geological storage. Three main value chains have been investigated corresponding to each of the two capture sites individually and one combining the two capture sites all with the same sort of transport and storage. Calculation results are then presented as total CO2 footprint measured in tonnes of CO2 equivalent per tonne CO2 stored for the value chain of each capture site separately and for both capture sites combined. In other words, the results from the tool show the impact of the implementation and operation of the chain on the CO2 storage efficiency. In addition, all three alternatives above are estimated when utilizing the full capacity of the storage infrastructure, 1.5Mt/y by introducing CO2 from other sources. The capture from these other sources are not included in the footprint calculations presented. Generally, the Longship project shows very low CO2 footprint compared to projects studied elsewhere. The 3 main reasons for the low CO2 footprint are 1) waste heat or internal steam is utilized for the CO2 capture processes,,2) the Norwegian electricity mix have a very low CO2 footprint and 3) the project has had high focus on using low footprint energy and fuel alternatives wherever possible. Since both capture sites have an element of biologically based raw materials the impact of BioCCS is also discussed as Longship can lead to net negative emissions. more...
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- 2021
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191. The impact of a closed-loop thalamocortical model on the spatiotemporal dynamics of cortical and thalamic traveling waves
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Bhattacharya, Sayak, primary, Cauchois, Matthieu B. L., additional, Iglesias, Pablo A., additional, and Chen, Zhe Sage, additional
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- 2021
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192. Application of Numerical Simulation to the Cost Effective Improvement of Resin Transfer Molding for Engineered Composite Parts
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J.-P. Cauchois, Christophe Binetruy, Patricia Krawczak, Département Technologie des Polymères et Composites & Ingénierie Mécanique (TPCIM), École des Mines de Douai (Mines Douai EMD), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Ministère de l'Economie, des Finances et de l'Industrie, Institut Mines-Télécom [Paris] (IMT), Ecole nationale supérieure Mines-Télécom Lille Douai (IMT Lille Douai), Pôle de Plasturgie de l'Est (PPE), and S.V. Hoa, A. Johnston & J. Denault more...
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[SPI]Engineering Sciences [physics] ,Materials science ,Transfer molding ,Computer simulation ,Composite number ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,Composite material ,ComputingMilieux_MISCELLANEOUS ,[SPI.MECA.GEME]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanical engineering [physics.class-ph] ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience
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- 2020
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193. Functional characterization of ABCC8 variants of unknown significance based on bioinformatics predictions, splicing assays, and protein analyses: Benefits for the accurate diagnosis of congenital hyperinsulinism
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Alexandra Martins, Christine Bellanné-Chantelot, Pascaline Gaildrat, Emily A. Rex, Show Ling Shyng, Delphine Bouvet, Marine Cauchois-Le Mière, Omar Soukarieh, Jean Baptiste Arnoux, Thierry Frebourg, Cécile Saint-Martin, and Julien Buratti more...
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0303 health sciences ,RNA Splicing ,030305 genetics & heredity ,Computational Biology ,Computational biology ,Exons ,Biology ,Molecular diagnostics ,Sulfonylurea Receptors ,Exon skipping ,Article ,03 medical and health sciences ,Exon ,RNA splicing ,Genetics ,Humans ,Ectopic expression ,Congenital Hyperinsulinism ,Gene ,Genetics (clinical) ,Loss function ,030304 developmental biology ,Minigene - Abstract
ABCC8 encodes the SUR1 subunit of the β-cell ATP-sensitive potassium channel whose loss of function causes congenital hyperinsulinism (CHI). Molecular diagnosis is critical for optimal management of CHI patients. Unfortunately, assessing the impact of ABCC8 variants on RNA splicing remains very challenging as this gene is poorly expressed in leukocytes. Here, we performed bioinformatics analysis and cell-based minigene assays to assess the impact on splicing of 13 ABCC8 variants identified in 20 CHI patients. Next, channel properties of SUR1 proteins expected to originate from minigene-detected in-frame splicing defects were analyzed after ectopic expression in COSm6 cells. Out of the analyzed variants, seven induced out-of-frame splicing defects and were therefore classified as recessive pathogenic, whereas two led to skipping of in-frame exons. Channel functional analysis of the latter demonstrated their pathogenicity. Interestingly, the common rs757110 SNP increased exon skipping in our system suggesting that it may act as a disease modifier factor. Our strategy allowed determining the pathogenicity of all selected ABCC8 variants, and CHI-inheritance pattern for 16 out of the 20 patients. This study highlights the value of combining RNA and protein functional approaches in variant interpretation and reveals the minigene splicing assay as a new tool for CHI molecular diagnostics. more...
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- 2020
194. Circulating Endothelial Cells as a Marker of Endothelial Injury in Severe COVID -19
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Thomas Robert, Mélanie Velier, Raphael Cauchois, Guillaume Lano, Philippe Brunet, Clara Degioanni, Laurent Papazian, Florence Daviet, Léa Pietri, Marie Koubi, Gilles Kaplanski, Yael Berda, Christophe Guervilly, Françoise Dignat-George, Sami Hraiech, Evelyne Abdili, Stéphane Burtey, Romaric Lacroix, Laurent Arnaud, Noémie Jourde-Chiche, Florence Sabatier, Hôpital Nord [CHU - APHM], Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de néphrologie et transplantation rénale [Hôpital de la Conception - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Laboratoire d'Immunologie [Hôpital de la Conception - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION)-Centre National de la Recherche Scientifique (CNRS), Centre d'études et de recherche sur les services de santé et la qualité de vie (CEReSS), Aix Marseille Université (AMU), Aix-Marseille University Institut National de la Sante et de la Recherche Medicale (Inserm) European CommissionAssistance Publique-Hopitaux de Marseille, and Prémilleux, Annick more...
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Adult ,Male ,0301 basic medicine ,circulating endothelial cells ,Endothelium ,medicine.medical_treatment ,[SDV]Life Sciences [q-bio] ,030204 cardiovascular system & hematology ,law.invention ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,law ,In vivo ,Intensive care ,Cell Adhesion ,Humans ,Immunology and Allergy ,Medicine ,Chronic exertional compartment syndrome ,Aged ,Retrospective Studies ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,SARS-CoV-2 ,business.industry ,Brief Report ,COVID-19 ,Retrospective cohort study ,Middle Aged ,endothelial injury ,medicine.disease ,Intensive care unit ,3. Good health ,[SDV] Life Sciences [q-bio] ,Intensive Care Units ,AcademicSubjects/MED00290 ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Cytokine ,Immunology ,Female ,Endothelium, Vascular ,business ,Biomarkers ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Beside the commonly described pulmonary expression of the coronavirus disease 2019 (COVID-19), major vascular events have been reported. The objective of this study was to investigate whether increased levels of circulating endothelial cells (CECs) might be associated with severe forms of COVID-19. Ninety-nine patients with COVID-19 were enrolled in this retrospective study. Patients in the intensive care units (ICU) had significantly higher CEC counts than non-ICU patients and the extent of endothelial injury was correlated with putative markers of disease severity and inflammatory cytokines. Together, these data provide in vivo evidence that endothelial injury is a key feature of COVID-19. more...
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- 2020
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195. Urgences vitales intra-hospitalières et urgences vitales extra-hospitalières admises en réanimations: le pronostic est-il différent ? Une étude de cohorte rétrospective monocentrique
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Cauchois, Emi, Aix-Marseille Université - École de médecine (AMU SMPM MED), Aix-Marseille Université - Faculté des sciences médicales et paramédicales (AMU SMPM), Aix Marseille Université (AMU)-Aix Marseille Université (AMU), and Julien Carvelli more...
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Urgence vitale intra-hospitalière ,[SDV]Life Sciences [q-bio] ,Réanimation ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Chaîne de survie intra-hospitalière - Abstract
Introduction : la Chaîne de Survie Intra-Hospitalière (CSIH) est désormais une organisation incontournable de la prise en charge des urgences vitales intra-hospitalières (UVIH) au sein des établissements de santé. Peu de données épidémiologiques sont disponibles au sujet des UVIH admises en réanimation. L’objectif de cette étude est de déterminer si le pronostic des patients de réanimation dépend de leur mode d’admission : intra-hospitalier via l’équipe médicale de CSIH ou extra-hospitalier via les systèmes d’urgence pré-hospitaliers (on parle d’urgences vitales extra-hospitalières, UVEH). Résultats : parmi les 422 admissions en réanimation (Réanimation du Centre hospitalo-universitaire de la Timone), 241 patients ont été inclus de façon rétrospective (2019-2020) : 74 UVIH et 167 UVEH. La mortalité intra-hospitalière n’était pas significativement différente entre les deux cohortes (n = 31(42%) contre 63(39%) respectivement, NS). Les UVIH étaient plus âgées et plus comorbides (immunodépression et cancer actif). Les UVEH présentaient des défaillances d’organe plus graves à l’arrivée, et nécessitaient plus fréquemment le recours à une ventilation mécanique. Les facteurs de risque de mortalité hospitalière retrouvés sont le cancer actif (OR = 10.4 [2.7-40], p < 0.001), les scores IGS II (OR = 1.05 [1.03-1.08], p < 0.0001) et SOFA (OR = 1.14 [1.01-1.3], p < 0.05), l’accident vasculaire cérébral (AVC) hémorragique comme diagnostic d’admission (OR = 8.4 [2.7-26], p < 0.001) et le taux de lactate artériel à l’arrivée (OR = 1.11 [1.03-1.2], p < 0.01). Conclusion : cette étude offre une analyse complète et approfondie des caractéristiques et du pronostic des patients admis en réanimation suite à l’activation d’une CSIH maîtrisée, comparés au mode d’admission extra-hospitalier. Malgré la plus grande fragilité des UVIH, il n’existe pas de différence en termes de mortalité hospitalière. Ce résultat encourage le recours précoce à des systèmes de réponse rapide aux urgences vitales intra-hospitalières pour identifier les patients qui s’aggravent en service. more...
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- 2020
196. Dissemination of extreme levels of extracellular vesicles: tissue factor activity in patients with severe COVID-19
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Françoise Dignat-George, Evelyne Abdili, Raphael Cauchois, Gilles Kaplanski, Christophe Guervilly, Léa Pietri, Laurent Papazian, Romaric Lacroix, Laurent Arnaud, Florence Sabatier, Jacques Albanèse, Thomas Robert, Amandine Bonifay, Mélanie Velier, Guillaume Lano, Stéphane Burtey, Hôpital Nord [CHU - APHM], Assistance Publique - Hôpitaux de Marseille (APHM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), and Lucas, Nelly more...
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[SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology ,0301 basic medicine ,Male ,Pilot Projects ,Disease ,030204 cardiovascular system & hematology ,Gastroenterology ,Severity of Illness Index ,chemistry.chemical_compound ,0302 clinical medicine ,Aged, 80 and over ,[SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology ,Hematology ,Middle Aged ,Thrombosis ,3. Good health ,Plasminogen activator inhibitor-1 ,Area Under Curve ,Cohort ,Female ,medicine.symptom ,Risk ,medicine.medical_specialty ,Inflammation ,macromolecular substances ,Thromboplastin ,Fibrin Fibrinogen Degradation Products ,03 medical and health sciences ,Extracellular Vesicles ,Internal medicine ,Severity of illness ,Plasminogen Activator Inhibitor 1 ,medicine ,Coagulopathy ,Humans ,Aged ,Proportional Hazards Models ,Septic shock ,business.industry ,SARS-CoV-2 ,COVID-19 ,medicine.disease ,Stimulus Report ,030104 developmental biology ,Logistic Models ,chemistry ,ROC Curve ,business - Abstract
Key Points EV-TF activity is dramatically increased in patients with severe COVID-19 and is associated with an increased thrombotic risk. Compared with patients with septic shock, those with severe COVID-19 display a distinct EV profile with higher procoagulant activity., Coronavirus disease 2019 (COVID-19) has become one of the biggest public health challenges of this century. Severe forms of the disease are associated with a thrombo-inflammatory state that can turn into thrombosis. Because tissue factor (TF) conveyed by extracellular vesicles (EVs) has been implicated in thrombosis, we quantified the EV-TF activity in a cohort of hospitalized patients with COVID-19 (n = 111) and evaluated its link with inflammation, disease severity, and thrombotic events. Patients with severe disease were compared with those who had moderate disease and with patients who had septic shock not related to COVID-19 (n = 218). The EV-TF activity was notably increased in patients with severe COVID-19 compared with that observed in patients with moderate COVID-19 (median, 231 [25th to 75th percentile, 39-761] vs median, 25 [25th to 75th percentile, 12-59] fM; P < .0001); EV-TF was correlated with leukocytes, D-dimer, and inflammation parameters. High EV-TF values were associated with an increased thrombotic risk in multivariable models. Compared with patients who had septic shock, those with COVID-19 were characterized by a distinct coagulopathy profile with significantly higher EV-TF and EV-fibrinolytic activities that were not counterbalanced by an increase in plasminogen activator inhibitor-1 (PAI-1). Thus, this article is the first to describe the dissemination of extreme levels of EV-TF in patients with severe COVID-19, which supports the international recommendations of systematic preventive anticoagulation in hospitalized patients and potential intensification of anticoagulation in patients with severe disease. more...
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- 2020
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197. Evaluation of the therapeutic potential of mesenchymal stromal cells during in vitro production: search for markers related to immunomodulatory properties
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M. El Ouafy, Naceur Charif, D. Ghannoum, Ghislaine Cauchois, A. Notrantonio, M. Rubio, N. de Isla, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Service d'Hématologie [CHRU Nancy], and Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy) more...
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Cancer Research ,Stromal cell ,[SDV]Life Sciences [q-bio] ,Immunology ,Cell ,Biology ,Flow cytometry ,Cell therapy ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Wharton's jelly ,medicine ,Immunology and Allergy ,Genetics (clinical) ,030304 developmental biology ,0303 health sciences ,Transplantation ,medicine.diagnostic_test ,Mesenchymal stem cell ,Cell Biology ,Cell biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Stem cell - Abstract
International audience; Background & AimMesenchymal stromal/stem cells (MSCs) are a heterogeneous population characterized by self-renewing and ability to differentiate into mesodermal cell origin. Wharton Jelly MSC (WJ-MSC) are a promising candidate for cell therapy due to their ease of isolation and their tissue regeneration capabilities. Moreover, they can regulate the immune response by secreting a set of soluble factors and by establishing direct interactions with the cells of immune system. These immunomodulation properties are used in the treatment of different pathologies such as graft versus host disease. In order to use WJ-MSCs for clinical applications, an in vitro expansion step is critical to obtain the sufficient dose. This step imposes several physicochemical and hydrodynamic constraints that can influence the quality and functional properties of cells.The objective of this work was to identify and validate predictive markers related to immunomodulatory properties of WJ-MSCs. For this purpose, CD119, CD200 and Notch1 were chosen and studied.Methods, Results & ConclusionAt first, markers distribution was studied by flow cytometry. Secondly, immunomodulatory potential of WJ-MSC was evaluated by a coculture of these cells with CD4, CD8 lymphocytes, followed by the study of lymphocytes proliferation by flow cytometry. These experimentss were performed under different conditions: WJ-MSCs cultivated in normoxia (N) and hypoxia (H), early passage and senescent cells, and under stimulation with gamma interferon (IFN-γ).Our results showed a heterogeneity of distribution of the markers studied. In addition, our study revealed that WJ-MSC cultivated in H was more immunosuppressive than those cultivated in N. Moreover, we showed that the cellular senescence induced a reduction of the immunosuppressive properties, and that IFN-γ stimulation increased WJ-MSC immunosuppression potential. Finally, our study highlighted a trend towards a correlation between the expression of CD119 and Notch1 by WJ-CSM and their immunomodulatory properties.To conclude, the culture conditions and cell senescence affect the immunomodulatory properties of WJ-CSM hence the interest of verifying these properties after the cell expansion procedures. Furthermore CD119 and Notch1 receptors may be predictive markers of the immunomodulatory properties of WJ -CSM to be checked during in vitro amplification. more...
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- 2020
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198. Evaluation of fetal mesenchymal stromal/stem cells senescence during in vitro amplification for therapeutic purpose: choice of cell quality parameters
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Naceur Charif, N. de Isla, D. Ghannoum, Ghislaine Cauchois, M. El Ouafy, Emilie Roeder, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), and Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) more...
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Senescence ,Cancer Research ,Stromal cell ,[SDV]Life Sciences [q-bio] ,Immunology ,Cell ,Biology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Immunology and Allergy ,Genetics (clinical) ,030304 developmental biology ,0303 health sciences ,Transplantation ,Cell growth ,Mesenchymal stem cell ,CD44 ,Cell Biology ,Cell biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,CD146 ,Stem cell - Abstract
International audience; Background & AimMesenchymal stromal/stem cells (MSCs) are fibroblast-like multipotent cells capable of proliferation, self-renewal and differentiation into different mesodermal cell-lineages. They are known to be implicated in many key biological processes like tissue regeneration, secretion of bioactive factors which give them immunomodulatory and trophy properties. MSCs isolated from umbilical cord Wharton's Jelly (WJ-MSC) aroused much attention due to several advantages: multipotency capacity, painless, safety for allogenic therapeutic purposes and efficiency for treating certain diseases. In order to use MSCs for therapeutic purpose, these cells must be expanded in long-term, which inevitably triggers cell senescence, a process of cell proliferation arrest that occurs in vivo and in vitro as a response of cells to excessive stresses. This phenomenon is characterized by several markers and features and it can affect cell behavior.Because of senescent cells influence the outcome of a variety of physiological and pathological processes, and because of WJ-MSCs are used in cell therapy, the aim of the study was to characterize senescence WJ-MSCs after in vitro expansion, to control the quality of these cells in terms of function and phenotype.Methods, Results & ConclusionTo induce senescence process, we used in vitro model called “replicative senescence”. Cells were cultured in normoxia (N) and hypoxia (H) conditions and analyzed in early and late passages. Proliferation analysis of MSCs demonstrated a high rate of proliferation in H condition. In addition, senescence analysis using a Beta-Galactosidase test indicated that MSCs in H condition senesce slower than those in N. On the other hand, cell-surface biomarker analysis revealed that all MSCs derived from WJ expressed the common surface markers among which CD44, CD105 without great changes during expansion. However other markers presented changes during expansion (like a decrease in CD146). Senescent cells presented variable immunosuppressive potential compared to MSCs in early passage. Known that senescent cells are characterized by changes in chromatin structure, we studied proteins implicated in chromatin remodeling like HMGB1. Immunofluorescence study showed a translocation of HMGB1 from MSC nucleus in senescent cells. All these results will improve the characterization of MSCs senescence and may provide new parameters for establishing the quality of MSCs prior to their therapeutic application. more...
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- 2020
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199. Fatal acetaminophen poisoning with hepatic microvesicular steatosis in a child after repeated administration of therapeutic doses
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Bruno Turlin, Renaud Bouvet, Alain Baert, Aurélie Cauchois, Bernard Fromenty, Isabelle Morel, Thomas Gicquel, Institut du droit public et de la science politique (IDPSP), Université de Rennes (UR), CHU Pontchaillou [Rennes], Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1) more...
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Pediatric acute-liver ,medicine.medical_specialty ,Necrosis ,failureMicrovesicular steatosis ,[SDV]Life Sciences [q-bio] ,Encephalopathy ,Microvesicular Steatosis ,Respiratory chain ,01 natural sciences ,Gastroenterology ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Painkiller toxicity ,medicine ,030216 legal & forensic medicine ,Fulminant hepatitis ,Hepatitis ,medicine.diagnostic_test ,business.industry ,Pediatric acute-liver failure ,010401 analytical chemistry ,Microvesicular steatosis ,medicine.disease ,3. Good health ,0104 chemical sciences ,Acetaminophen ,Death ,Liver biopsy ,Acetaminophen-induced hepatotoxicity ,medicine.symptom ,business ,Law ,medicine.drug - Abstract
International audience; Acetaminophen is the leading cause of acute liver failure worldwide following massive ingestion. We present here a fatal acute liver failure after repeated administration of four therapeutic doses of acetaminophen at 4-h intervals in a previously healthy 9-year-old female who presented dental pain after a facial trauma during sport practice. A diagnosis of paracetamol-induced hepatitis was deduced from the clinical picture of fulminant hepatitis and tubular necrosis, the encephalopathy with oedema and without signs of trauma. Liver biopsy showed typical acetaminophen-induced necrosis and a microvesicular steatosis in periportal hepatocytes. These injuries might have been favored by pre-existing mitochondrial dysfunction related, for instance, to a deficiency in an enzyme of the mitochondrial β-oxidation pathway, or the respiratory chain. The observation of microvesicular steatosis in the periportal areas suggests an increased vulnerability via pre-existing mitochondrial dysfunction. As the liver status of patients is mostly unknown, the frequency of administration (every six hours) must be respected and the use of pharmaceutical forms allowing to adjust the dose as closely as possible to the child's weight should be promoted. more...
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- 2020
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200. Effect of anakinra on mortality in patients with COVID-19: a systematic review and patient-level meta-analysis
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Kyriazopoulou, E., Huet, T., Cavalli, G., Gori, A., Kyprianou, M., Pickkers, P., Eugen-Olsen, J., Clerici, M., Veas, F., Chatellier, G., Kaplanski, G., Netea, M.G., Pontali, E., Gattorno, M., Cauchois, R., Kooistra, E., Kox, M., Bandera, A., Beaussier, H., Mangioni, D., Dagna, L., Meer, J.W.M. van der, Giamarellos-Bourboulis, E.J., Hayem, G., Kyriazopoulou, E., Huet, T., Cavalli, G., Gori, A., Kyprianou, M., Pickkers, P., Eugen-Olsen, J., Clerici, M., Veas, F., Chatellier, G., Kaplanski, G., Netea, M.G., Pontali, E., Gattorno, M., Cauchois, R., Kooistra, E., Kox, M., Bandera, A., Beaussier, H., Mangioni, D., Dagna, L., Meer, J.W.M. van der, Giamarellos-Bourboulis, E.J., and Hayem, G. more...
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Item does not contain fulltext, BACKGROUND: Anakinra might improve the prognosis of patients with moderate to severe COVID-19 (ie, patients requiring oxygen supplementation but not yet receiving organ support). We aimed to assess the effect of anakinra treatment on mortality in patients admitted to hospital with COVID-19. METHODS: For this systematic review and individual patient-level meta-analysis, a systematic literature search was done on Dec 28, 2020, in Medline (PubMed), Cochrane, medRxiv, bioRxiv, and the ClinicalTrials.gov databases for randomised trials, comparative studies, and observational studies of patients admitted to hospital with COVID-19, comparing administration of anakinra with standard of care, or placebo, or both. The search was repeated on Jan 22, 2021. Individual patient-level data were requested from investigators and corresponding authors of eligible studies; if individual patient-level data were not available, published data were extracted from the original reports. The primary endpoint was mortality after 28 days and the secondary endpoint was safety (eg, the risk of secondary infections). This study is registered on PROSPERO (CRD42020221491). FINDINGS: 209 articles were identified, of which 178 full-text articles fulfilled screening criteria and were assessed. Aggregate data on 1185 patients from nine studies were analysed, and individual patient-level data on 895 patients were provided from six of these studies. Eight studies were observational and one was a randomised controlled trial. Most studies used historical controls. In the individual patient-level meta-analysis, after adjusting for age, comorbidities, baseline ratio of the arterial partial oxygen pressure divided by the fraction of inspired oxygen (PaO(2)/FiO(2)), C-reactive protein (CRP) concentrations, and lymphopenia, mortality was significantly lower in patients treated with anakinra (38 [11%] of 342) than in those receiving standard of care with or without placebo (137 [25%] of 553; adjusted odds ratio [ more...
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- 2021
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