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Background. Melittin is a major constituent of honeybee venom and comprises a water-soluble surfactant peptide with cytolytic effects potentially applicable in anticancer therapy. We evaluated the impact of melittin from Bashkir honeybee (Apis mellifera mellifera L.) venom on cell viability of various prostate cancer lineages.Materials and methods. MTT assays with cell viability index estimation were used to evaluate the effect of melittin on cell proliferation in various-grade malignancy prostate cancer (PC) lineages, LNCaP, PC-3 and DU145.Results and discussion. Lineage DU145 revealed a low sensitivity to melittin, because a relatively high peptide concentration of 10 μg/mL had a suppressive effect on its proliferation. With PC-3 cells, a 0.1 μg/mL concentration suppressed proliferation significantly to 46.15 %, while melittin at a 10 μg/mL dose had a cytolytic effect on most cells (4.27 % viability). LNCaP cells experienced the lowest toxicity at 10 μg/mL melittin compared to PC-3 and DU145 lineages. The LNCaP, PC-3 and DU145 PC lineages demonstrated suppressed proliferation at melittin levels 0.01–100 μg/mL.Conclusion. The study reveals a significant reduction of the PC lineages viability at a minimal melittin concentration of 0.01 μg/mL, which indicates a high cytolytic activity of this peptide and renders it a candidate agent in antitumour therapy.

Congenital hemangiomas are benign vascular lesions found in fetuses. Congenital hemangiomas develop in utero, have a maximum size at birth (in the absence of subsequent bleeding). Such hemangiomas occur in 1–2% of newborns. Prenatal ultrasound diagnostics and magnetic resonance imaging occupy an important place in the diagnosis, which determines the tactics of management and treatment of the patient. To present a clinical case of prenatal diagnosis of congenital soft tissue hemangioma of the fetal back. A pregnant woman A., 34, was sent to St. Petersburg State Public Health Institution «Diagnostic Center» (medical and genetic) with suspected spina bifida of the fetus after screening in late pregnancy period in one of the Birth Centres. Performed prenatal ultrasound (US) pregnant A. for a period of 34 weeks. According to ultrasound data in the region of the spine at the level of XI–XII thoracic vertebrae, an ovoid formation with clear even contours, a fairly homogeneous solid structure, with a clear capsule, 33×24×30 mm in size (volume 12.4 cm3) is determined subcutaneously. The diagnosis made: hemangioma of the soft tissues of the fetal back. Prenatal screening plays a key role in the diagnosis of congenital hemangiomas. Timely detection of this benign formation affects the tactics of management and delivery.

Today in Ukraine the issue of defining land as an element in the system of land management, cadastre and evaluation remains relevant. The term "land" means a part of the land that has clearly defined boundaries, a certain location, natural properties, physical parameters, it also describes the legal and economic status. The main properties of the land plot can be considered: boundaries, area, cadastral number and address, as well as legal properties, namely the rights and obligations of the land owner, restrictions and encumbrances, land easements. In addition to these characteristics, there are also so-called quality, they include: the cost of use or exchange. When a land plot is formed, it becomes an object of civil law, and it is for this purpose that it is formed. Nowadays, with the creation of the land market, it is necessary that every owner or user of land has the right to draw up documents necessary for the sale or lease of land, and this includes work on the formation of land, which is also a job. on drawing up according to the project of land management. The purpose of the article is to study the principles and technological aspects of land formation for further creation of land management documentation. The study presents the principles and technological aspects of land formation for their further evaluation in the structure of existing land use and land tenure. The directions of development of technical documentation in the system of land management and cadastre are determined. The mechanism of development of land management projects taking into account modern legal restrictions and market needs is analyzed. It is proved that the necessary condition of land management is to take into account the principles of data collection, processing and preparation of technical documentation. Particular attention is paid to defining modern principles of development of urban planning documentation, based on the latest developments in the industry. The researched issues in the article make a significant contribution to the areas of land management design, formation of cadastral data, development and updating of urban planning documentation and more.

A land plot is a part of the land that has clearly defined boundaries, a certain location, natural properties, physical parameters, it also describes the legal and economic status. The main properties of the land plot can be considered: boundaries, area, cadastral number and address, as well as legal properties, namely the rights and obligations of the land owner, restrictions and encumbrances, land easements. In addition to these characteristics, there are also so-called quality, they include: the cost of use or exchange. When a land plot is formed, it becomes an object of civil law, and it is for this purpose that it is formed. Nowadays, with the creation of the land market, it is necessary that every owner or user of land has the right to draw up documents that are necessary for the sale or lease of land, and this includes work on the formation of land, and it is work on drawing up a land management project in accordance with. Depending on the available materials from the previous works on this site, the composition and scope of work required for the formation of the site are determined. These characteristics are also related to the reform, or in other words, the reorganization of agricultural enterprises. Two other factors that affect the composition and scope of work are the number and location of real estate to be transferred. There are also many other factors of influence. The purpose of the article is to study the principles of development of land management projects, taking into account the scheme of application of tools for the organization and protection of land. The paper analyzes modern domestic and international approaches to the development of land management projects and the formation of land management documentation at different levels and for different applications. The technology and systematized stages of creating a land management project are determined. Factors influencing the location of the real estate object are analyzed. It is determined that all the presented factors must be taken into account at all stages of the construction, starting with its construction and ending with its operation. But taking into account each individual factor in the efficiency of use or profitability of the building, if it is commercial, is not enough to choose the right location of the property. The principles of formation of land management documentation for construction purposes are determined. The developed directions in research will give the chance to increase land use by application of modern technological schemes of development of land management projects taking into account current requirements of the legislation.

The purpose of the research is to analyze and summarize the literature data and the results of our own studies on the use of drug delivery systems and methods for increasing the solubility and efcacy of anthelmintic drugs.Helminthoses are one of the most important problems in veterinary medicine and animal husbandry, not only due to the pathological effects, but also due to the spread of anthelmintic resistance. Since the development of new anthelmintic substances takes many years and investment, some strategies are currently focused on modifying existing drugs to increase their efcacy, reduce side effects and overcome anthelmintic resistance. Literature data analysis on various methods and means of increasing the solubility and efcacy of anthelmintic drugs was carried out and the prospects for their use, including using modern nanotechnological methods of drug delivery were given.

The purpose of the research is to study effects of the 2.0% supramolecular complex of ivermectin in overdoses on the clinical condition of horses.Materials and methods. The experiment was conducted on 20 horses of different breeds in the Seradin Equestrian Center, the North Caucasus Federal District, the Chechen Republic, in October 2020. The horses were divided according to the principle of analogues into 3 test groups and one control group of 5 animals each. The 2.0% supramolecular complex of ivermectin was administered to the horses from group 1 at a therapeutic dose of 0.2 mg/kg; group 2 received a tripled dose of 0.6 mg/kg, and the group 3, a 5-fold increased dose of 1,0 mg/kg for the active substance. The drug was administered once individually in a mixture with compound feed. The body temperature was measured with a non-contact thermometer, and the pulse rate and the respiratory rate were measured per 1 min. Urine of the horses was examined on days 1, 7 and 14 after the drug. The urine color, transparency and consistency were determined. Multifunctional test paper was used to study pH, protein, glucose, ketone bodies, nitrites, bilirubin, and urobilinogen. Blood was taken in the morning from the horses’ jugular vein on days 1, 7 and 14 after the drug. Hematological and biochemical parameters were studied on analyzers.Results and discussion. Clinical fndings of the horses (body temperature, pulse and respiration) did not differ from those of the control animals after the 2.0% supramolecular complex of ivermectin was administered in a therapeutic, tripled and 5-fold increased doses on days 1, 7 and 14. A slight increase in ketone bodies was observed in the urine on day 1, which may indicate the drug effect on the central nervous system at a 5-fold increased dose; all parameters were within the normal range on days 7 and 14. The 2.0% supramolecular complex of ivermectin in overdoses (0.6; 1.0 mg/kg for the active substance) did not adversely affect the horses’ hematological or biochemical blood parameters.

The purpose of the research is to study the features of the mechanochemical modification of medicinal substances with low solubility in water. According to the Biopharmaceutics Classification System, about 6% of medicinal substances belong to the group of insoluble medicinal substances, and about 47%, to practically insoluble medicinal substances, i.e. more than a half substances have problems with solubility, and therefore bioavailability and pharmacological activity. To achieve the therapeutic effect of such substances, it is necessary to deliberately increase the dosage of the substance, which increases the cost of the drug, as well as safety risks of pharmacotherapy. The work evaluated possibilities of mechanochemical modification of a number of known anthelmintic substances with low solubility to increase this parameter. It was shown that during machining of such substances in the presence of polymeric substances, solid dispersions were formed that had increased solubility while maintaining high anthelmintic activity with decreased dosage of the active substance. Preparations in the form of solid dispersions can be used both orally and by a group method to 10-20 animals, mixed with compound feed. For low-melting substances, a liquid-phase machining method is proposed to obtain stable suspension concentrates that are convenient for oral administration. The prospects for obtaining mixed compositions based on two substances are shown, which makes it possible to obtain combined preparations with a wide spectrum of action with the minimized volume and frequency of their use.

The purpose of the research is to study the chemical stability of triclabendazole (TCB) in preparations obtained by its mechanochemical modification with polymeric substances and succinic acid (SA), as well as samples of Triclafascid obtained in 2015–2022 and left for storage at a temperature of 18–25 0C and a relative humidity of 55–60%. To achieve this goal, we selected samples of preparations obtained earlier and in freshly prepared solid dispersions (SD) based on TCB, SA, polyvinylpyrrolidone (PVP) and arabinogalactan (AG). Modification of TCB by polymeric substances was carried out at a weight ratio of components 1:9 in an LE101 ball mill at a rotation speed of 65–70 rpm with sampling for stability (integrity) analysis of TCB by high performance liquid chromatography (HPLC). The integrity analysis of TCB in its SDs obtained in 2015–2022 showed that there was no chemical destruction of TCB in them. The TCB substance is a stable chemical molecule that is not affected by destruction either during machining or during succinic acid treatment. The obtained SDs remain stable for TCB during storage for more than 7 years.

Among the most common and dangerous parasitic diseases of animals and humans is fascioliasis. The most effective against Fasciola is triclabendazole ("Fasinex 5.0%" suspension), imported by Siba-Geigi, which is practically not used in Russia due to its high cost. Considering the huge economic damage caused by fascioliasis, we have developed a new complex preparation using innovative mechanochemical technology based on the substance of triclabendazole "Triclafascid", which is 5 times more active than the substance of triclabendazole against fascioliasis. The drug is an intermolecular complex in the form of an easily flowing solid dispersed powder with a size of 0.1–10 microns. The drug is administered orally once to cattle and sheep for therapeutic and prophylactic purposes for acute and chronic fascioliasis, to sheep at a therapeutic dose of 2.0 mg/kg and to cattle 2.5 mg/kg for the AI, respectively, and 20 and 25 mg /kg for the drug, once individually in the form of an aqueous solution. In case of mass deworming of animals, it is convenient to give the anthelmintic by a group method to 10–20 animals, mixed with mixed feed at a dose of 3.0 mg/kg for the AI (for the preparation 30 mg/kg).

The article describes the importance of physical education in human life, {"references":["1. Adizov B. R., Khamroev A. R. MODELING ACTIVITIES OF TEACHERS WHEN DESIGNING CREATIVE ACTIVITIES OF STUDENTS //ILMIY XABARNOMA. -С. 69. 2. Alisherovich M. N. HEALTH AND PHYSICAL EDUCATION IN EDUCATIONAL INSTITUTIONS TECHNOLOGY OF DEVELOPMENT MEASURES //Конференции. - 2020. 3. Kh K. R., Shukurov R. S., Samadov S. S. The Inquiry On The Changes Of Ralations Towards The Basics Of The Culture Of Healthy Life In The Students Of Elective Courses //International Journal of Progressive Sciences and Technologies. - 2021. - Т. 25. - №. 2. - С. 112-118."]}

This article describes the pulse, respiration and body temperature of 4-5 month-old lambs when using trout liver extract for experimental purposes.

Planar superstructural defects have a great influence on the mechanical, functional properties of binary ordered alloys of the L10 superstructure based on the fcc lattice, but there is no complete analysis of their structure and energy in the literature. For the L10 superstructure alloys of the stoichiometric composition AB, the paper gives the expressions for calculating the sublimation energy and the energy of a planar superstructural defect in the model of hard coordination spheres and pair interatomic interactions. The crystal lattice tetragonality was not taken into account. The authors presented the ordered alloy structure as a union of four monoatomic simple cubic lattices, two of which are occupied by A atoms, and the other two by B atoms. This approach allows calculating the sublimation energy required for crystal evaporation. The first eight coordination spheres were taken into account in the work. The paper shows an algorithm for determining all possible geometrically different representations of the L10 superstructure with the same sublimation energy, gives an expression for finding the planes of occurrence of all possible conservative antiphase boundaries. The study identified that the conservative and nonconservative antiphase boundaries, as well as conservative and nonconservative boundaries of C-domains are observed in the binary ordered alloys of the L10 superstructure based on the fcc lattice. The algorithms described in this work make it possible to carry out a crystal-geometric analysis of planar defects in both binary and multicomponent ordered alloys with various superstructures.

The healthcare system is designed to ensure the preservation and improvement of the health of the nation by providing highly qualified medical and preventive care, which is impossible today without the use of modern types of medical equipment. Every year around the world there is a steady increase in the presence of abnormalities in infants. In their diagnosis, the leading role belongs to studies using a fetal monitor. This is a modern, highly informative diagnostic method that allows you to detect defects in fetal development at an early stage.

Актуальность исследования обусловлена необходимостью разработки требований к сульфированным битумам (асфальтам), применяемым в составе буровых растворов на водной основе, для контроля их качества при поступлении на буровые предприятия. Вследствие наличия водонерастворимых веществ сульфированные битумы считаются гидрофобными кольматантами глинистых пород, хотя на самом деле доля водорастворимых веществ в их составе значительно выше (~70 %). На сегодняшний день неизвестно влияние водорастворимой составляющей сульфированных битумов на глинистую породу с точки зрения ингибирования и пептизации. Также отсутствуют данные о солестойкости и устойчивости к щелочноземельным металлам (Ca2+, Mg2+) сульфированных битумов. Все это может приводить к нерациональному их применению. Изучение этих вопросов поможет сформулировать требования и подобрать методы оценки качества сульфированных битумов, а также улучшить эффективность их использования в составе буровых растворов. Цель: экспериментально определить функциональные свойства сульфированных битумов в составе буровых растворов на водной основе. Задачи: изучить влияние сульфированных битумов на глины и оценить их устойчивость к минерализации и двухвалентным катионам. Объекты: образцы сульфированных битумов различных марок и заводов-производителей. Методы. Технологические параметры глинистых буровых растворов определялись стандартными методами в соответствии с ГОСТ 33213-2014 (ISO 10414-1:2008); ингибирующие и пептизирующие свойства объектов исследования по отношению к глинистой породе изучали методом набухания глины при нахождении ее в исследуемом растворе (на приборе Жигача - Ярова) и посредством седиментационного анализа (на весах Фигуровского); солестойкость сульфированных битумов определяли методом осадкообразования при нахождении их в минерализованной среде. Результаты. Проведен сравнительный анализ шести образцов сульфированных битумов для выявления физико-химического влияния реагентов данной группы на глинистую породу. Посредством оценки степени набухания и определения размера частиц глины при нахождении ее в растворах исследуемых образцов установлено, что существует две группы реагентов сульфированных битумов, противоположных по функциональному воздействию на глину: 1) пептизаторы (дефлокулянты); 2) ингибиторы набухания. Экспериментально доказано, что все сульфированные битумы способствуют ухудшению тиксотропных, реологических и фильтрационных свойств пресных глинистых систем, при этом более эффективными понизителями вязкости являются образцы, обладающие ингибирующими свойствами; в минерализованных средах и при наличии двухвалентных катионов сульфированные битумы также способствуют ухудшению фильтрационных свойств, но неэффективны как понизители вязкости, и даже наоборот, способствуют сильному загущению, что связано с их коагуляцией, или снижением растворимости. Установлено, что для определения функционального применения высокомолекулярных сульфированных битумов в составе буровых растворов на водной основе и контроля качества образцов необходимо испытывать их по следующим показателям: определять размер частиц глины в воде и в растворе испытуемого образца с целью выявления его ингибирующих или пептизирующих свойств; оценивать разжижающую способность и эффективность снижения фильтрационных свойств при добавлении реагента к пресной глинистой суспензии. The relevance of the work stems from the need to develop requirements for sulfonated bitumen applied in the composition of water-based drilling fluids to control their quality when entering drilling enterprises. Due to the presence of water-insoluble substances, sulfonated bitumens are considered to be hydrophobic bridging agents of clay rocks, though the proportion of water-soluble substances in their composition is much higher (~70 %). Nowadays the influence of the watersoluble component of sulfonated bitumen on clay rock in terms of inhibition and peptization is unknown. There are also no dat a on salt resistance and resistance to alkaline earth metals (Ca2+, Mg2+) of sulfonated bitumen. All this can lead to their irrational use. The study of these issues will help formulate requirements and select methods for assessing the quality of sulfonated bitumens, as well as improve the efficiency of their use in drilling fluids. The main aim of the research is to experimentally determine the functional properties of sulfonated bitumen in the composition of waterbased drilling fluids. The main objectives of the research are to study the sulfonated bitumen effect on clay rocks and access the sulfonated bitumen resistance to mineralization and divalent cations. Objects: sulfonated bitumen samples of various brands and manufacturing plants. Methods. The technological parameters of clay drilling fluids were determined by standard methods in accordance with SS 33213-2014 (ISO 10414-1:2008); the inhibitory and peptization properties of the research objects in relation to clay rocks were studied by the meth od of clay swelling when they were in the sample solution (on the Zhigach-Yarov tester) and by means of sedimentary analysis (on the Figurovsky weight scale); salt resistance of sulfonated bitumens was determined by the method of sedimentation while placing sulfonated bitumens in a mineralized environment. Results. The authors have carried out the comparative analysis of six sulfonated bitumen samples to identify the physicochemical effect of this group reagents on clay rocks. By assessing the degree of swelling and particle-size determination of clay while its staying in the drilling fluids of the test samples, two groups of reagents of sulfonated bitumen were found, which are opposite in their functional effect on clay: 1) peptizing agents (deflocculants); 2) clay inhibitors. It was experimentally proved that all sulfonated bitumens contribute to the deterioration of the thixotropic, rheological, and filtration properties of fresh clay systems, while samples with inhibitory properties are more effective viscosity reducers; in a mineralized environment, the presence of divalent cations and sulfonated bitumens are conductive to a decrease in filtration properties, but are ineffective as viscosity reducers, and also, they are conductive to thickening due to their coagulation or solubility decrease. Experiments proved that for determining the functional purpose of high-molecular-weight sulfonated bitumens in the composition of water-based drilling fluids and controlling the quality of the samples, it is necessary to test them according to the following indicators: to determine the size of clay particles in water and in a solution of the test sample in order to identify its inhibitory or peptizing properties; to evaluate the thinning capacity and the effectiveness of reducing the filtration characteristics when adding a reagent to a fresh clay suspension.

A model of the formation of the gradient sea-level field by the space bi-static radar measurements is developed. An interferometer radar with cross-sectional base is used as a receiver. The relatively small antenna 5–10 m of cross-sectional base gives a huge gain in energy when working for quasi-mirror scattering in the range of short wind waves. The efficiency of the system is evaluated by converting the dynamical model of tsunami wave evolution for the case of the Kuril earthquake (October 4, 1994) into a panoramic radar image of the sea level. The panoramic image of the front wave allows predicting the direction, amplitude and, finally, the expected time of tsunami arrival to a given point. The obtained radar image confirms the main feature of the quasi- mirror method: the fluctuation-level sensitivity varies within the radar swath (~2000 km) and is the worst near the mirror point. For the chosen radar parameters, the average sensitivity in the swath is ~5 cm for a site (15 × 15) km. Without accounting the time required to transmit information from the receiver to the tsunami–prone sites, the minimal time interval between the appearance of the wave front and the tsunami alert is determined by the number of sequentially launched small tandem spacecrafts. For a single tandem this time is about 45 min.