OBJECTIVE: To probe into the mechanism of gynecological Tiaoqi Jiedu formula on chronic endometritis. METHODS: Through network pharmacology, the possible key targets and key pathways of gynecological Tiaoqi Jiedu formula on endometritis were identified. On the basis of this theory, the mouse model of endometritis was established and divided into the blank group, model group, western medicine group (levofloxacin hydrochloride), and low-dose group, medium-dose group and high-dose group (low-dose, medium-dose and high-dose gynecological Tiaoqi Jiedu formula). The network pharmacology results were verified by enzyme-linked immunosorbent assay (ELISA), real-time fluorescent quantitative polymerase chain reaction (PCR) and Western blotting. RESULTS: A total of 219 compounds and 698 corresponding targets were screened from gynecological Tiaoqi Jiedu formula. A total of 36 key targets were obtained by intersections with endometritis targets. Seventy-seven metabolic pathways were obtained by Kyoto encyclopedia of genes and genomes enrichment, including nuclear factor κB ( NF-κB) signaling pathway, advanced glycation end products (AGE)-AGE receptor signaling pathway, tumor necrosis factor signaling pathway and interleukin (IL)-17 signaling pathway. ELISA results showed that the levels of MPO, IL-6 and IL-1β in the model group were significantly higher than those in the blank group (P<0. 01). Compared with the model group, the lowdose gynecological Tiaoqi Jiedu formula group had a significant reduction in IL-6, with statistically significant difference (P<0. 05), but there was no significant difference in MPO and IL-1β between two groups (P> 0. 05). Compared with the model group, MPO, IL-6 and IL-1β in the medium-dose group, high-dose group and western medicine group decreased significantly,with statistically significant differences (P< 0. 01). The results of real-time fluorescence quantitative PCR and Western blotting showed that the gene and protein expression of P-P65 and TLR4 in the uterine tissue of the model group were significantly higher than those of the normal group, with statistically significant differences ( P < 0. 01 or P < 0. 001). Compared with the model group, the low-dose group could not significantly inhibit the expression of P-P65 and TLR4 mRNA and protein (P>0. 05), while the expression of P-P65 mRNA and TLR4 mRNA in the medium-dose group, high-dose group and western medicine group decreased significantly (P<0. 05 or P<0. 01, P<0. 001), the expression of P-P65 protein and TLR4 protein in the high-dose group and western medicine group decreased significantly (P<0. 01 or P<0. 05), the differences were statistically significant. There was no significant difference in the expression of P65 mRNA and protein in each group ( P > 0. 05). CONCLUSIONS: Gynecological Tiaoqi Jiedu formula can significantly down-regulate the levels of inflammatory factors and inhibit the expression of key genes in TLR4/ NF-κB signaling pathway in the mouse model of lipopolysaccharide (LPS)-induced endometritis, so as to achieve the purpose of anti-inflammatory and endometrial protection. [ABSTRACT FROM AUTHOR]