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【Objective】The efficient and simple methods for the preparation of four lipopeptide antibiotics produced by Bacillus subtilis strains were established and the biological functions of these four lipopeptide antibiotics were elucideted. 【Method】The four family lipopeptide antibiotics surfactin, bacillomycin, fengycin and locillomycin were prepared by acid precipitation, organic solvent extraction and solid phase extraction from the culture broth of different Bacillus subtilis 916 and its mutants; The purities of the four lipopeptides were detected by HPLCMS and Tricine-SDS-PAGE; The antifungal, antibacterial and hemolytic activities of the four lipopeptide antibiotics were analyzed by Fusarium oxysporum, Staphylococcus aureus and sheep red blood cells. 【Result】The results of HPLC-MS and Tricine-SDS-PAGE showed that all the four family lipopeptide antibiotics prepared by this study reached electrophoresis pure and chromatography pure. Investigation of the biological functions of the four class lipopeptides showed that surfactin and bacillomycin L had strong haemolytic activities, but fengycin and locillomycin only had weak haemolytic activities, while bacillomycin L and fengycin showed strong antifungal activities, but surfactin and locillomycin showed antibacterial and antiviral activities. 【Conclusion】In general, the efficient methods for purification of the four lipopeptide antibiotics surfactin, bacillomycin L, fengycin and locillomycin from Bacillus subtilis was set up and their biological activities were elucideted, which laid a solid foundation to further elucidate the structure function relationship, biosynthesis pathway and application of these lipopeptide antibiotics. [ABSTRACT FROM AUTHOR]

Copyright of Yellow River is the property of Editorial Board of Yellow River and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of West China Journal of Stomatology is the property of Sichuan University, West China College of Stomatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nursing of Integrated Traditional Chinese & Western Medicine is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Central China Normal University is the property of Huazhong Normal University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Computer Measurement & Control is the property of Magazine Agency of Computer Measurement & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Based on push-out tests of 13 partially concrete encased composite members with H-web welded studs, the failure modes, load-slip behavior and longitudinal shear-transfer behavior of these members were investigated. The main parameters examined included the number of studs, the diameter of studs, the layout method of studs, the width of steel flange, the arrangement of stirrups as well as the loading schemes. The experimental results indicate that, these specimens fail in the mode of stud shear failure or the mode of concrete encasing steel web split failure. The typical load-slip curves can be divided into three characteristic stages, namely, no slip stage, load ascending stage and load descending stage. I he shear studs on the web of profile steel can effectively increase the longitudinal shear capacity of these specimens, and the shear capacity increases with the innre§se nt Ilif «fnd Hiameter and the number of studs. I he longitudinal arrangement of shear studs performs better than the transverse arrangement of shear studs. The longitudinal eccentric arrangement of shear studs has little effect on the shear capacity. The specimen with wide flange steel can restrain the web concrete and improve the shear capacity. The effect of stirrups on the shear capacity is small when the web stirrups are arranged regularly. By analyzing the mechanical mechanism and the shear transfer mode, a simple model is proposed for predicting the longitudinal shear capacity of partially concrete encased composite members with shear studs. It shows that the calculated results agree well with the experimental results. [ABSTRACT FROM AUTHOR]

Copyright of Nankai Journal: Philosophy, Literature & Social Science Edition / Nankai Xuebao: Zhexue Shehui Kezue Ban is the property of Nankai Xuebao Bianjibu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao is the property of Journal of Nanjing Agricultural University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Computer Measurement & Control is the property of Magazine Agency of Computer Measurement & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Pharmaceutical Practice & Service is the property of Journal of Pharmaceutical Practice Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In terms of the teacher-flow policy of compulsory education, regular multiway rotation is laid stress on in Japan and national overall distribution and individual demand are taken into consideration in France. Although it is different in the scope and procedure of teacher flow, there are some common essentials of the policy in the two countries: emphasizing government-leading, teacher’s identity of "system "not of"school", the coerciveness of teacher flow, careful and overall classification of teacher-flow participants.These common essentials can be used for reference to perfect the teacher-flow policy in China. [ABSTRACT FROM AUTHOR]

Copyright of Tourism Tribune / Lvyou Xuekan is the property of Tourism Institute of Beijing Union University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the related factors of atrial fibrillation for hospitalized patients with type 2 diabetes mellitus and hypertension ertension. Methods: 112 cases of atrial fibrillation patients combined with type 2 diabetes mellitus and hypertension were taken as the research objects, and, named as atrial fibrillation group (n=112). At the same time, 150 cases of patients with type 2 diabetes mellitus and hypertension whose age and gender were matched with the atrial fibrillation group but had no atrial fibrillation were selected as control group (n=150). The clinical data and laboratory examination indexes were compared, and factors associated with atrial fibrillation complication were analyzed by using Logistic regression equation. Results: Compared with control group, patients in atrial fibrillation group had high systolic blood pressure (SBP), high proportion of hypertension, low proportion of taking ACEI/ARB drugs (P<0.05), low left ventricular ejection fraction (LVEF) (P<0.05), long left atrial diameter (LAD) (P<0.05), and high levels of triglycerides (TC), low-density lipoprotein cholesterol (LDL-C), glycosylated hemoglobin (HbA1c), serum creatinine (Scr), b-type brain natriuretic peptide (BNP), high-sensitivity c-reactive protein (hs CRP) and uric acid (P<0.05). Multiariable Logistic regression equation analysis showed that LAD,HbA1c, BNP, hs CRP and uric acid were independent risk factors of patients complicated with atrial fibrillation (P<0.05), while taking ACEI/ARB drugs was protective factors. Conclusion: LAD, HbA1c, BNP, hs CRP and uric acid may be independent risk factors for patients with type 2 diabetes mellitus and hypertension complicated with atrial fibrillation. [ABSTRACT FROM AUTHOR]

Copyright of Computer Engineering & Science / Jisuanji Gongcheng yu Kexue is the property of Computer Engineering & Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的建立一种烟熏诱导的慢性阻塞性肺疾病(COPD)小鼠模型,并对其进行验证。方法将32只C57BL/6小鼠随机分为正常组、烟熏2周组、烟熏4周组、恢复组,各8只。正常组不予烟熏,烟熏2、4周组及恢复组置于熏烟箱中,被动吸烟6支/次,每次1 h;两次间隔30 min呼吸新鲜空气,4次/d,5 d/周。烟熏2周组第10天停止吸烟,烟熏4周组第26天停止吸烟,恢复组烟熏4周后再正常饲养2周。观察各组一般情况及体质量变化,测定支气管肺泡灌洗液(BALF)中蛋白含量;取肺组织制备切片,HE染色观察病理形态变化及肺泡直径。结果建模开始后正常组体质量呈上升趋势,烟熏2、4周组及恢复组出现精神萎靡、食欲降低,体质量较同时间点正常组降低(P均<0.01);恢复组停止烟熏后体质量缓慢上升,但仍低于正常组(P均<0.05)。BALF蛋白含量:烟熏2、4周组及恢复组均明显高于正常组(P均<0.01);烟熏4周组及恢复组明显高于烟熏2周组,恢复组明显低于烟熏4周组(P均<0.01)。肺组织病理形态学显示,正常组肺组织有少量淋巴细胞,肺泡结构完整;烟熏2周组有少量中性粒细胞、大量单核细胞及淋巴细胞浸润,肺泡扩大,部分肺泡间隔断裂并融合;烟熏4周组肺组织伴有少量中性粒细胞浸润,单核细胞及淋巴细胞浸润较烟熏2周组明显增多;支气管上皮细胞变性、坏死脱落,部分肺泡间隔变窄、断裂并融合成肺大疱。肺泡直径:烟熏2、4周组及恢复组均大于正常组,烟熏4周组及恢复组均大于烟熏2周组(P均<0.01),烟熏4周组与恢复组比较差异无统计学意义(P>0.05)。结论单纯烟熏4周法成功制备COPD小鼠模型;该模型符合人类COPD的病理和功能改变,是进行COPD动物实验的理想建模方法。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To compare the inhibitory effects of butorphanol and dezocine on etomidate-induced myoclonus. Methods A total of 150 patients with ASA physical statusⅠorⅡ, aged 40~65 yr, with body mass index (BMI) of 20~ 25 kg/m2, scheduled for elective operations under general anesthesia, were included in this study. Patients were randomly allocated into three groups (A, B and C) with 50 patients in each group. Group A was given intravenous butorphanol 15 μg/kg for 30 s, Group B was given dezocine 0.1 mg/kg and Group C was given equal volume of saline. After 2min, etomidate 0.3 mg/ kg was administrated to three groups. The occurrence and severity of myoclonus were recorded for 2 min after administration of etomidate. The mean arterial pressure (MAP), heart rate (HR), pulse oxygen saturation (SpO2) and Bispectral index (BIS) were recorded at the time points before induction (T0), 2 min after the experimental drug treatment (T1), and 2 min after etomidate treatment (T2). At the same time, the concentration of serum potassium was determined at T0 and 5 min after endotracheal intubation (T3) respectively. Results The positive incidences of myoclonus were 12% in group A, 22% in group B and 74% in group C, respectively. Compared with group C, the positive incidence rates of myoclonus and myoclonus scales were significantly lower in group A and group B (P < 0.05), but no significant difference between group A and group B (P > 0.05). Compared with T0, there was no significant difference in the potassium concentration between patients without myoclonus (grade 0) and patients with myoclonus (grade 1 and grade 2) at T3 (P < 0.05). There was significantly increased in potassium concentration in patients with grade 3(P < 0.05). There were no significant differences in MAP, HR, SpO2 and BIS values at T0, T1 and T2 between three groups of patients(P＞0.05). Conclusion Pretreatment of butorphanol (15 μg/kg) or dezocine (0.1 mg/kg) can reduce the etomidate-induced myoclonus. At the same time, both of treatment shows no different effects on circulation and respiration system. [ABSTRACT FROM AUTHOR]

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Osteoporosis is the property of Chinese Journal of Osteoporosis and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To study the characteristics of shedding herpes simplex virus (HSV) in patients with subclinical genital herpes, and provide basis for clinical therapy. Methods A total of 142 patients with subclinical genital herpes were obtained in our hospital from June 2014 to June 2016. The fluorescent quantitation polymerase chain reaction was used to detect the HSV-DNA, and enzyme linked immunosorbent assay (ELISA) was applied to check the type of serum IgG. The effects of age, gender, antibody types, disease courses and occurrence frequency on the positive rate of HSV DNA shedding were analyzed. Results The positive rate of HSV-DNA in 142 patients was 49.3%, and females showed significantly higher HSV shedding rate than males (P < 0.05). There was no significant difference in HSV shedding rate between patients with serum HSV-Ⅰ IgG++HSV-Ⅱ IgG+ and patients with HSV-Ⅱ IgG+ alone, while both of which showed higher HSV shedding rate than those of patients with HSV-Ⅰ IgG+ alone. Patients with short infection period (≤6 years) showed significantly higher HSV shedding rate than patients with longer infection period (>6 years). HSV shedding rate in patients with high frequent occurrence was found to be significantly higher than that in patients with low frequent occurrence (P < 0.05). In patients who showed short infection period (≤6 years), the HSV shedding rate was found significantly higher in frequent patients (recurrence ≥ 6 times/year) than that in few occurrence patients (recurrence < 6 times/year). There was no significant difference in HSV shedding rate in patients with recurrent occurrence when they went through more than 6 years' HSV infection (P > 0.05). More patients with positive HSV-Ⅱ IgG were found in female than that in male (P < 0.05). Conclusion In patients with subclinical genital herpes, HSV shedding rate is closely related to gender, serum antibody type, disease course and recurrent occurrence. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Traditional Chinese Medicine is the property of Journal of Beijing University of Traditional Chinese Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

急性缺血性卒中是导致急性脑组织损伤的重要原因，是中老年人致残、死亡的主要因素，可挽救缺血组织的早期发现是临床及时干预治疗的关键。随着功能磁共振成像技术的发展，临床可从水分子扩散、血流动力学、氧合水平、pH值、代谢及侧支循环等方面全方位评价脑组织的病理改变，为疾病进展及预后评估提供更全面的信息。本文综述急性缺血性卒中缺血性半暗带功能磁共振成像的研究进展，以期为卒中患者的评估和治疗计划提供影像学支撑与指导。 Abstract: Acute ischemic stroke is one of the most important causes of acute brain injury, and early detection of salvaged ischemic brain tissue is the key to timely clinical intervention. With the development of functional magnetic resonance imaging (fMRI), the pathological changes of brain tissue can be comprehensively evaluated from multiple aspects of water molecular diffusion, hemodynamics, oxygenation, pH, metabolism and collateral circulation, which provide more information for evaluating disease progression and prognosis. The article reviewed the progress of fMRI in evaluating ischemic penumbra in acute ischemic stroke, so as to provide imaging evaluation reference for stroke diagnosis and treatment.

目的 探讨总结老年患者肢体抖动型短暂性脑缺血发作（limb-shaking transient ischemic attack，LSTIA） 的临床特点及发病机制。 方法 采用回顾性分析，收集2013年1月-2017年6月在芜湖市第一人民医院神经内科住院确诊的21 例LS-TIA患者的临床资料及治疗方案。 结果 21例患者均表现为一侧肢体短暂性、发作性不能控制的不自主抖动，所有患者均存在抖 动肢体对侧的颅内和（或）颅外大动脉重度狭窄或闭塞（100%）。患者住院期间均行磁共振成像 （magnetic resonance imaging，MRI）及磁共振血管成像（magnetic resonance angiography，MRA）检查及常 规检查，并接受抗血小板、强化他汀治疗，适当控制血压，积极治疗原发病，临床症状均明显缓解， 随访期间均未再出现TI A发作，无脑血管事件发生。 结论 一侧肢体抖动时应警惕LS-TI A可能。LS-TI A患者多存在对侧的颅内外动脉重度狭窄或闭塞，应 对此类患者完善相关血管检查，进行卒中二级预防。 Abstract: Objective To summarize the clinical characteristics and pathogenesis of limb-shaking transient ischemic attack (LS-TIA) in elderly patients, and to provide reference for clinicians. Methods The general clinical data and treatment regimen of 21 LS-TIA elderly patients in Wuhu hospital from January 2013 to June 2017 were collected, and a retrospective analysis was performed to summarize the clinical characteristics of LS-TIA. Results All of the 21 elderly patients presented with unilateral limb transient and uncontrollable involuntary shaking. All patients had contralateral intracranial and/or extracranial arteries severe stenosis or occlusion diagnosed by MRI+DWI+MRA examination. All patients were given antiplatelet and intensive statin therapy, proper control of blood pressure and treatment of primary diseases. After the treatment, clinical symptoms of all patients were alleviated, and no TIA and other cerebrovascular events occurred during the follow-up. Conclusions LS-TIA should be considered when occurring unilateral limb paroxysmal shaking. In general, LS-TIA patients have contralateral intracranial and/or external arteries stenosis or occlusion, and these patients should be given aggressive stroke secondary prevention.