101. Capsid Integrity Detection of Enteric Viruses in Reclaimed Waters.
- Author
-
Puchades-Colera, Pablo, Díaz-Reolid, Azahara, Girón-Guzmán, Inés, Cuevas-Ferrando, Enric, Pérez-Cataluña, Alba, and Sánchez, Gloria
- Subjects
- *
ENTEROVIRUSES , *WATER reuse , *VIRUS inactivation , *FECAL contamination , *WASTE recycling , *WATER sampling , *BACTERIOPHAGES - Abstract
Climate change, unpredictable weather patterns, and droughts are depleting water resources in some parts of the globe, where recycling and reusing wastewater is a strategy for different purposes. To counteract this, the EU regulation for water reuse sets minimum requirements for the use of reclaimed water for agricultural irrigation, including a reduction in human enteric viruses. In the present study, the occurrence of several human enteric viruses, including the human norovirus genogroup I (HuNoV GI), HuNoV GII, and rotavirus (RV), along with viral fecal contamination indicator crAssphage was monitored by using (RT)-qPCR methods on influent wastewater and reclaimed water samples. Moreover, the level of somatic coliphages was also determined as a culturable viral indicator. To assess the potential viral infectivity, an optimization of a capsid integrity PMAxx-RT-qPCR method was performed on sewage samples. Somatic coliphages were present in 60% of the reclaimed water samples, indicating inefficient virus inactivation. Following PMAxx-RT-qPCR optimization, 66% of the samples tested positive for at least one of the analyzed enteric viruses, with concentrations ranging from 2.79 to 7.30 Log10 genome copies (gc)/L. Overall, most of the analyzed reclaimed water samples did not comply with current EU legislation and contained potential infectious viral particles. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF