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Copyright of Urologie Pro Praxi is the property of SOLEN sro and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction. The sufficiency of vitamin D is important for slowing down the aging of the skin, maintaining its hydration, elasticity and the ability to regenerate. Biologically active forms of vitamin D (including alfacalcidol) promote the activation of genes whose function is directly related to the maintenance of the structure of the skin, subcutaneous tissue, fascia and muscle fibers.The aim of the work was to assess the prospects for the inclusion of alfacalcidol (“Alpha D3”, 0.25 μg, 1 caps/day) in the rehabilitation programs of patients with age-related ptosis of the face (n = 40, age 47 ± 5 years).Materials and methods. 4 groups of patients with age-related facial ptosis (n = 40, average age 47 ± 5 years) were observed for 60 days; measurements were made before and after clinical trials. Group 1A (first treatment, n = 12) received the daily dose of Alpha D3, 0.25 mg, in the morning, for 60 days, during this period the patients received 4 cosmetic procedures (2 plastic face massages and 2 stimulation current therapies). Group 1B (first control, n = 8) received only 4 cosmetic procedures over 60 days (2 plastic face massages and 2 stimulation current therapies). Group 2A (second treatment, n = 12) received the daily dose of ALFA D3, 0.25 mg, for 60 days, during this period the patients received 4 DMAE (diethylaminoethanol) mesotherapy procedures. Group 2B (second control, n = 8) only received 4 DMAE (diethylaminoethanol) mesotherapy procedures.Results. Alfacalcidol intake significantly increased the concentrations of 25 (OH) D (from 17 ± 5 ng/ml to 27 ± 8 ng/ml, P = 0.001) and calcium (from 86 ± 10 mg/L to 96 ± 6 mg/L, P = 0.01) in serum. The positive dynamics of the concentrations of 25 (OH) D and calcium when taking alfacalcidol corresponded to an increase in the total bone mineral density (+ 0.03 ± 0.03 g/cm3, control: + 0.006 ± 0.03, P = 0.016) and T-criterion (+0.4 ± 0.5, control: -0.07 ± 0.2, P = 0.0002), which indicates compensation for vitamin D deficiency and an improvement in bone metabolism. An increase in the levels of 25 (OH) D and calcium when taking alfacalcidol was accompanied by a positive trend in skin condition according to bioimpedance measurements. Taking the drug significantly increased the moisture content of the facial skin (from 17 ± 14 points to 29 ± 14 points, P = 0.055, without changes in the control) and increased the amplitude of the muscle motor response to the stimulus (+ 0.24 ± 0.22, P < 0.02).Conclusions. Within the framework of a randomized design, it was shown that the addition of massage, microcurrent therapy, mesotherapy with alfacalcidol led to a significant increase in serum 25 (OH) D levels, an increase in skin elasticity and hydration, a decrease in visceral fat according to bioimpedance measurements, and an improvement in indicators of muscle contractility and neuromuscular signal transmission according to electromyography data and an increase in bone mineral density.

Recently, interest in lactobacilli lysates is growing, and the possibilities of their use cover more and more areas of human life – medicine, immunoprophylaxis, cosmetology, food industry. The article presents a method of obtaining bacterial lysates of lactic acid bacteria, Lactobacillus genus. As a destructive agent used dry lytic enzyme preparation сytal-Rk G-10X, obtained under conditions of experimental fermentation from the culture fluid Streptomyces albus UN44. The complex lytic preparation сytal-Rk contains a group of enzymes glycosidases and peptidases, the joint action of which leads to the degradation of the cell wall of a wide range of bacteria. The evaluation of the effectiveness of the use of Cytal-Rk for the degradation of six strains of lactobacilli is presented. It is shown that the enzyme is an effective destructive agent under optimal conditions. The degree of cell degradation depended on their species, hydrolysis conditions and physical condition. Optimal conditions for obtaining hydrolysates based on native and lyophilized cells of L. delbrueckii subsp. bulgaricus LB86 was develop. When loading the microbial mass in the reaction medium – 1×109 CFU/ml (for native cells) and 1×1010 CFU/ml (for lyophilized cells) cell destruction reached almost 80% and 90%, respectively. Based on native Lactobacillus delbrueckii subsp. bulgaricus LB86 lysates were obtained and their comparative chemical analysis was performed. Sublimated cells were shown to be more sensitive to the enzyme complex and to contain more proteins and reducing sugars. Native cell lysates were more enriched in nucleic acids. Lysate of lactic acid bacteria from native cells was studied to moisturize the skin of the hands of young people aged 18–20 years. When using lysate in the cream base, it significantly increased the level of hydration of the dermis of the hands compared to the control cream base. This allows us to consider the lysate of Lactobacillus delbrueckii subsp. bulgaricus LB86 as a promising ingredient for creating cosmetics with moisturizing action.

Background: As a traditional Chinese medicine, the Colla corii asini (CCA), obtained from the skin of Equus animus L, has been used to nourish blood in China for more than 2000 years. The beneficial role of CCA supplementation in beauty has been reported in different case studies; however, research supporting these claims remains sparse. Objectives: To conduct a 12-week randomized, controlled, open-labeled, blinded assessment trial to clinically evaluate the effects of CCA on hydration, complexion, wrinkles, and elasticity of human skin. Methods: Participants (n=90) were randomly assigned to receive either no supplement or 6 g of CCA once daily for 12 weeks. Parameters evaluating skin hydration, complexion, wrinkles, and elasticity were assessed at baseline and after 4, 8, and 12 weeks of treatment. Results and Conclusions: Compared with the control group, skin-hydration values were significantly higher in the CCA group after 8 and 12 weeks (P=0.002, P=0.007, respectively). After 12 weeks, in the CCA group, the melanin index was significantly decreased compared with the control group (P=0.013). In terms of the safety of CCA, none of the participants presented adverse symptoms related to the CCA during the study period. These results suggest that CCA is an effective supplement for the improvement of skin hydration and to brighten skin color.

Introduction: According to the Polish Central Statistical Office and the National Cancer Registry, cancer is the main cause of death in the world. Every year over 8 million people die from cancer worldwide, while in Poland the disease kills nearly 100,000 people. The group of patients analysed in this study suffered from breast, colorectal and lung cancers, and underwent radio- and chemotherapy. The patients were treated with a cosmetic formulation to accelerate the healing of their skin and improve the functioning of the hydrolipidic barrier. Aim: To examine oncological patients' skin and to improve its biophysical parameters with a specialized cosmetic preparation. Material and methods: The study was conducted on 60 female cancer patients treated at the University Hospital in Zielona Gora, Poland. The patients volunteered for in vivo tests of the cosmetic formulation. Results: The emulsions applied to the patients undergoing chemotherapy significantly increased the hydration of their epidermis. After 4 months of regular use of the preparation, their skin hydration level increased by 22%. The skin hydration of the patients undergoing radiotherapy increased by 3%, as compared with the levels at the beginning of the study. Conclusions: Cosmetic formulations for oncological patients restore the adequate level of skin hydration, aid the reconstruction of the skin and its protective barrier, soothe irritations, accelerate the healing of pathological lesions and prevent their development. Adverse effects may delay or prevent further treatment. Therefore, appropriately tailored care is very important and should not be overlooked. [ABSTRACT FROM AUTHOR]

Background: Nowadays, face masks are a crucial part of our daily life. Previous studies on their impact on the skin usually focused on the adverse effects of face masks. Few studies have assessed their influence on skin characteristics. In a previous study, we identified the short‐term effects of wearing face masks. Herein, we describe the long‐term skin effects of face masks, for a period of 6 months. Materials and methods: Healthy volunteers (19 men and women), who wore face masks, participated in the study from June 2020 to December 2020. In all participants, skin characteristics such as trans‐epidermal water loss (TEWL), skin hydration, skin elasticity, skin pore area, skin keratin amount, skin temperature, skin redness, skin temperature, skin redness, and skin color were measured three times. Results: TEWL, skin hydration, skin elasticity, skin pore area, skin keratin amount, and skin color changed significantly after 6 months. TEWL, skin hydration, skin pore area, skin keratin amount, and skin color were significantly different between the mask‐wearing and non‐mask‐wearing areas. Conclusion: Long‐term daily use of face masks can alter skin characteristics. Special care should be focused on the mask‐wearing regions. [ABSTRACT FROM AUTHOR]

Skin is our barrier against environmental damage. Moisturizers are widely used to increase hydration and barrier integrity of the skin; however, there are contrasting observations on their in vivo effects in real‐life settings. In cosmetic studies, corneometers and tewameters are traditionally used to assess skin hydration. In this study, two novel noninvasive diagnostic techniques, optical coherence tomography (OCT) and confocal Raman spectroscopy, were used to analyze stratum corneum and epidermal thickness (ET), water content, blood flow in function of depth, skin roughness, attenuation coefficient, natural moisturizing factor, ceramides and free fatty acids, cholesterol, urea, and lactates in 20 female subjects aged between 30 and 45 before and after 2 weeks application of a commercially available moisturizing lotion on one forearm. The untreated forearm served as control. A third measurement was conducted 1 week after cessation of moisturizing to verify whether the changes in the analyzed parameters persisted. We noticed a reduction in skin roughness, an increase in ceramides and free fatty acids and a not statistically significant increase in ET. As a conclusion, short time moisturizing appears insufficient to provide significant changes in skin morphology and composition, as assessed by OCT and RS. Novel noninvasive imaging methods are suitable for the evaluation of skin response to topical moisturizers. Further studies on larger sample size and longer treatment schedules are needed to analyze changes under treatment with moisturizers and to standardize the use of novel noninvasive diagnostic techniques. [ABSTRACT FROM AUTHOR]

Background Exposure to chemicals in batik process increases the risk of skin barrier disruption in batik craftswomen. Methods In the control group, the stratum corneum hydration was significantly increased at the evaluation of the 2nd week (P=0.045; CI 95%) and the 4th week (P=0.010; CI 95%). In the experimental group there was a significant decrease in TEWL value at the 2nd week evaluation (P=0.004; CI 95%), a significant increase in stratum corneum hydration at the 2nd week (P <0.001; CI 95%) and 4th week evaluation (P<0.001; CI 95%), and significant skin pH at 4th week (P=0.014; CI 95%). Results In the control group, the stratum corneum hydration was significantly increased at the evaluation of the 2nd week and the 4th week. In the experimental group there was a significant decrease in TEWL value at the 2nd week evaluation, a significant increase in stratum corneum hydration at the 2nd week and 4th week evaluation, and significant skin pH at 4th week. Conclusion Aloe vera benefits in improving the physiological function of the skin in Madurese batik craftswomen. [ABSTRACT FROM AUTHOR]

The cannabis plant is essentially a variety of the Cannabis Sativa L. plant. It is used in the textile, energy, automotive and cosmetics. The use of oil derived from hemp seeds has become widespread in cosmetics. Hemp seed oil does not contain ?9-tetrahydrocannabinol (?9-THC) and it is used in the production of food, biodiesel and polymers as well as cosmetics. In this study, different concentrations of hemp seed oil were used in cream and soap formulations, which are among the cosmetic products, and effect on skin hydration, sebum and transepidermal water loss (TEWL) on the skin was investigated. At the same time, the foaming test was applied to soaps containing different concentrations of hemp seed oil to see effects of soap foam performance. To analyse these, a rinse-off and leave-on product were selected. In soap formulations, there is positive effect on skin hydration and TEWL with sebum decrease. The most important change in cream formulations with respect to bar soap formulations is the increases in sebum. The effects of hemp seed oil on soap foam performance were examined. As a result of the study, its effect on the foam of the soap base which is used in this study is positive. [ABSTRACT FROM AUTHOR]

One of the most important characteristics of aging skin is dehydration, which is why the use of moisturizing products is very important, especially with increasing age. Thus, the user’s experience when using a product is interesting for the companies to develop specific cosmetics not only considering the physiological needs of each skin, but also according to the preference of a group if there is any. For this, a moisturizer was developed, containing an antioxidant active, ascorbyl tetraisopalmitate, whose sensory characteristics were evaluated by 33 Brazilian women between 30 and 60 years old. The results showed that the formulation was well accepted by all subjects, regardless of their age group, initial hydration, or the presence of visible signs of skin aging. It is suggested that the presence of the active ingredient in different concentrations caused a different perception of the formula for specific attributes such as the aqueous residue, film formation, and the feelings of oiliness and stickiness to the skin after application. These results suggest that the perception of the sensory characteristics of the product was more related to the nature and proportion of the compounds than to the age of the subjects.

Skin type classification is important because it provides guidance for professionals and consumers to recommend and select the most appropriate cosmetic products and skin care protocols and it is also important in clinical research. Several methods have been proposed for classifying skin typologies such as non-invasive bioengineering tools (examples: Corneometer® and Sebumeter®), visual and tactile methods (subjective methods that evaluate skin appearance, texture, temperature, and abnormalities), artificial intelligence-based tools and instruments (examples: visual rating scales, and self-report instruments). Examples of known visual rating scales used to classify skin aging are the Griffiths Photonumeric Scale, the Glogau Scale, and the SCINEXA Scale. The Fitzpatrick Skin Phototype Classification and the Baumann Skin Type System are some of the self-report instruments used for skin type classification. Despite the diversity of methods to classify skin type and degree of skin aging, data on instruments are scarce and not adequately compiled. Validation in larger samples and with individuals of different ethnicities and geographic locations is needed to promote a more universal use. Visual rating scales and instruments are interesting tools that allow the skin to be promptly and efficiently examined, without using costly or complex equipment, and are very useful in a clinical or self-assessment context.

The article presents modern data on the features of the structure of the skin and the formation of the epidermal barrier in premature and young children. Information is given regarding changes in the properties of the skin: microrelief, hydration, transepidermal water loss, acidity, content of the natural moisturizing factor, and sebum secretion. Attention is also paid to the role of vernix caseosa in the formation of a mature epidermal barrier and data on the use of emollients are provided.

Topical liquid formulations, dissolving microneedles (DMNs), and microscale needles composed of biodegradable materials have been widely used for the transdermal delivery of active compounds for skincare. However, transdermal active compound delivery by topical liquid formulation application is inhibited by skin barriers, and the skincare efficacy of DMNs is restricted by the low encapsulation capacity and incomplete insertion. In this study, topical serum application via a dissolvable micro-channeling system (DMCS) was used to enhance serum delivery through micro-channels embedded with DMNs. Transdermal serum delivery was evaluated after the topical-serum-only application and combinatorial serum application by assessing the intensity of allophycocyanin (APC) loaded with the serum in the porcine skin. APC intensity was significantly higher in the skin layer at a depth of 120–270 μm upon combinatorial serum application as compared to topical-serum-only application. In addition, the combinatorial serum application showed significantly improved efficacy in the clinical assessment of skin hydration, depigmentation, improvement of wrinkles, elasticity, dermal density, skin pores, and skin soothing without any safety issues compared to the serum-only application. The results indicate that combinatorial serum application with DMCS is a promising candidate for improving skincare treatments with optimal transdermal delivery of active compounds.

Lipid nanoparticles (LN) were invented in the early 1990ties and can be exploited for oral and topical drug delivery to increase the bioavailability of lipophilic active compounds. The lipid matrix of the LN can be composed of solid lipids or of a mixture of liquid and solid lipids. The influence of the lipid matrix composition of LN on the dermal penetration efficacy is not known and was therefore investigated in this study. For this the whole spectrum of LN, that means NE (100% liquid lipid), SLN (100% solid lipid) and NLC that contained low, medium and high amounts of oil were produced and characterized in regard to size, zeta potential, crystallinity and in-vitro release. In addition, the dermal penetration efficacy was determined ex-vivo and the bio-physical skin parameters, i.e., spreadability on skin, skin hydration, skin friction and transepidermal water loss were also assessed. Results demonstrate the tremendous influence of the lipid matrix composition on the biopharmaceutical properties of the LN but showed only minor differences in the physico-chemical properties of the particles. The physico-chemical properties of the LN and the in-vitro release data were not clearly linked to the dermal penetration efficacy, because also other parameters, e.g., skin hydration, spreadability of the formulation on skin and/or film formation of the LN on skin were found to be important parameters that influence the dermal penetration efficacy. Therefore, to allow for the development of effective LN formulations with tailor-made biopharmaceutical properties, not only the physico-chemical properties and in-vitro drug release profiles but also the most relevant biopharmaceutical properties of the LN should be assessed during the formulation development of LN. [Display omitted] [ABSTRACT FROM AUTHOR]

Recently, interest in lactobacilli lysates is growing, and the possibilities of their use cover more and more areas of human life – medicine, immunoprophylaxis, cosmetology, food industry. The article presents a method of obtaining bacterial lysates of lactic acid bacteria, Lactobacillus genus. As a destructive agent used dry lytic enzyme preparation сytal-Rk G-10X, obtained under conditions of experimental fermentation from the culture fluid Streptomyces albus UN44. The complex lytic preparation сytal-Rk contains a group of enzymes glycosidases and peptidases, the joint action of which leads to the degradation of the cell wall of a wide range of bacteria. The evaluation of the effectiveness of the use of Cytal-Rk for the degradation of six strains of lactobacilli is presented. It is shown that the enzyme is an effective destructive agent under optimal conditions. The degree of cell degradation depended on their species, hydrolysis conditions and physical condition. Optimal conditions for obtaining hydrolysates based on native and lyophilized cells of L. delbrueckii subsp. bulgaricus LB86 was develop. When loading the microbial mass in the reaction medium – 1×109 CFU/ml (for native cells) and 1×1010 CFU/ml (for lyophilized cells) cell destruction reached almost 80% and 90%, respectively. Based on native Lactobacillus delbrueckii subsp. bulgaricus LB86 lysates were obtained and their comparative chemical analysis was performed. Sublimated cells were shown to be more sensitive to the enzyme complex and to contain more proteins and reducing sugars. Native cell lysates were more enriched in nucleic acids. Lysate of lactic acid bacteria from native cells was studied to moisturize the skin of the hands of young people aged 18–20 years. When using lysate in the cream base, it significantly increased the level of hydration of the dermis of the hands compared to the control cream base. This allows us to consider the lysate of Lactobacillus delbrueckii subsp. bulgaricus LB86 as a promising ingredient for creating cosmetics with moisturizing action. [ABSTRACT FROM AUTHOR]

Copyright of Praktické Lékárenství is the property of SOLEN sro and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objectives: To evaluate the potency of a new skin hydration sensor patch in the fast self‐recording of skin hydration. Material and methods: The Skin Hydration Sensor Patch (SHSP) turns the user's smartphone into a wireless skin moisture measuring device. The SHSP combines a capacitive measurement unit and Near Field Communication technology (NFC) for transmitting data and energy. The probe is fixed onto the back of the smartphone and pressed to the skin for a few seconds where the application immediately calculates the capacitance value. Once recorded, the probe is then immediately taken off from the skin. In a first study, this system was compared to the Corneometer® technique, in vivo, on various skin sites of 23 healthy French women. In a second study, 20 women with moderate dry skin on face and forearm self‐recorded, through the SHSP the changes in skin hydration induced by a Xanthan gel containing 3% (w/w) of Glycerol, along 24 hours. A questionnaire based on 5 types of questions was established to be filled by subjects about their perception of the use of this new system. Results: In the first study, the values recorded by the SHSP were found highly correlated with those provided by the Corneometer®. The second study allowed to observe significant differences in skin hydration of both sites at all times, as compared to values obtained before the application of the gel. Differences between both sites were observed, the face being less hydrated than forearm. From a practical aspect, the self‐recordings on the face show a higher variability (approx. 10% than those of the forearm). The questionnaire led to positive answers on almost all points. Conclusion: This SHSP appears as a promising approach in the field of connected skin‐related devices. As such, it opens or enlarges a new paradigm in the relationships between a consumer and a cosmetic product. [ABSTRACT FROM AUTHOR]

Background: Various methods of bathing may affect skin properties differently. Aims: To compare the effects of immersion and showering on skin hydration and transepidermal water loss (TEWL). Method: This experimental study included healthy volunteers whose forearms were immersed and showered for 3 minutes. Skin hydration and TEWL were assessed serially before and after immersion and showering of volunteer forearms. Results: Seventy‐eight healthy volunteers (49 females, 29 males) were enrolled with an age range of 12‐55 years (mean 31.41 ± 10.33). Both methods significantly increased skin hydration and TEWL (P <.001). The capacitance value significantly increased immediately after bathing, and then rapidly decreased within 3 minutes. It returned to baseline by 10 minutes after bathing. There was no statistically significant difference of capacitance between the two methods at any measurement (P >.05). TEWL at every measurement after bathing was significantly increased compared to baseline for both bathing methods (P <.001). The highest TEWL was observed immediately after bathing, but then significantly decreased compared to the previous measurement (P <.001). Conclusion: Immersion and showering similarly demonstrated significant increase in skin hydration and TEWL. The increment of capacitance after bathing returned to baseline level within 10 minutes. [ABSTRACT FROM AUTHOR]

Background: M89 M (Mineral 89 mask, Laboratoires Vichy, France), containing 89% Vichy volcanic mineralizing water and hyaluronic acid, aims to strengthen and repair skin barrier. Aims: To assess the efficacy, tolerance, patient satisfaction, and quality of life (QOL) using M89 M after laser procedures (LP). Methods: M89 M was applied immediately post‐LP for 10 minutes, then daily for 5 days and 2‐3 times a week, up to 28 days on the faces of 51 women. Evaluations were performed immediately post‐LP, immediately after M89 M application at D0, D1, D5, and D28, and included criteria such as erythema and skin dryness. Subjects scored burning and warm sensations, itching, skin tightness, and stinging. Skin hydration using a Corneometer, skin barrier integrity using a Tewameter, and erythema using a Chromameter were assessed. Local tolerance and adverse events were recorded. After 28 days, subjects answered a questionnaire regarding the M89 M subjective cosmetic properties and QOL. Results: All subjects were in their mid‐forties with a phototype of II, III, or IV. M89 M significantly (P <.001) reduced the immediate cutaneous discomfort sensation and laser procedure‐related symptoms (burning, warmth sensation, itching/stinging, skin tightness). Skin hydration, and erythema, assessed using instrumental measures, were also significantly improved immediately after mask application (P ≤.01). Subjects highly appreciated M89 M and their QOL improved after 28 days of use. Local tolerance was good to excellent in both studies. Conclusion: M89 M is effective and safe immediately after esthetic procedures such as ablative and nonablative lasers and also improves the subject's QOL. [ABSTRACT FROM AUTHOR]

Traditional pumpkin (Cucurbita moschata) and Japanese pumpkin (C. maxima) consist of natural polysaccharides. From a scientific basis, natural polysaccharides could be applied to improve hydration in the cosmetic field. The purified polysaccharide was extracted and deproteinized with the CaCl2 method. Japanese pumpkin showed the higher value of physicochemical properties including yield (12.96 ± 0.60%), total polysaccharide content (0.89 ± 0.04 mg/mL), swelling capacity (4.00 ± 0.00%), swelling index (1.04 ± 0.00%), solubility (126.67 ± 5.77%), viscosity (1.25 ± 0.00 cps), water capacity (0.93 ± 0.15 g/g) and oil absorption capacity (5.93 ± 0.06 g/g) than traditional pumpkin. Additionally, Japanese pumpkin (IC50 9.30 ± 0.58 µg/mL) provided higher antioxidant activity by DPPH assay than traditional pumpkin (IC50 9.98 ± 0.25 µg/mL). The evaluation of efficacy on skin hydration in fifteen Thai volunteers indicated that Japanese pumpkin showed non-skin irritation. An extract concentration of 0.05–0.1% showed a significantly increased effect in moisturizing ability according to concentration (p < 0.05). This result supported that it was safe and effective to use as a moisturizer for cosmetic products.

Cryotherapy is becoming an increasingly popular method used in medicine, physiotherapy, and cosmetology. It is used in the form of whole-body cryotherapy (WBC) and local cryotherapy. It is a tool for achieving analgesic and anti-inflammatory effects. Since the beginning of its use, its influence on the mental state has also been pointed out. The aim of this study was to analyze the available literature on the effect of cryogenic temperatures on the skin and the mechanisms induced by such a stimulus and its influence on well-being. A literature search of keywords or phrases was performed in PubMed®. Various effects of WBC on skin characteristics (hydration, pH, level of transepidermal water loss), mechanisms of anti-inflammatory effects, and effects on adipocytes were shown. Research on the impact of individual skin characteristics is not consistent. Positive effects on the reduction of inflammation and oxidative stress have been noted. Cryotherapy is also successfully used in dermatology to treat lentil spots, actinic keratosis, and ingrown toenails, remove viral warts, or relieve itching in atopic dermatitis. The results of the review also indicate the effectiveness of WBC as an adjunctive treatment for obesity. The number of papers available on the direct effects of WBC on the skin is still limited, despite the fact that it represents the first contact of the human body with cryogenic temperatures. Available data show that cold as a physical stimulus can be a safe and useful tool in cosmetology.

This research describes a wearable skin hydration sensor based on cotton textile to determine the state of hydration within the skin via impedance analysis. The sensor structure comprises a textile substrate, thermoplastic over-layer, conductive patterns, and encapsulant, designed for stable and reliable monitoring of the skin’s impedance change in relation to hydration level. The porcine skin with different hydration levels was prepared as a model system of the skin, and the textile-based sensor carefully investigated the porcine skin samples’ impedance characteristics. The impedance study reveals that (1) the total impedance of skin decreases as its hydration level increases, and (2) the impedance of the stratum corneum and epidermis layers are more dominantly affected by the hydration level of the skin than the dermis layer. Even after repetitive bending cycles, the impedance data of skin measured by the sensor exhibit a reliable dependence on the skin hydration level, which validates the flexibility and durability of the sensor. Finally, it is shown that the textile-based skin hydration sensor can detect various body parts’ different hydration levels of human skin while maintaining a stable conformal contact with the skin. The resulting data are well-matched with the readings from a commercial skin hydration sensor.

The presence of water in the skin is crucial for maintaining the properties and functions of the skin, in particular its outermost layer, known as the stratum corneum, which consists of a lipid barrier. External exposures can affect the skin’s hydration levels and in turn, alter its mechanical and physical properties. Monitoring these alterations in the skin’s water content can be applicable in clinical, cosmetic, athletic and personal settings. Many techniques measuring this parameter have been investigated, with electrical-based methods currently being widely used in commercial devices. Furthermore, the exploration of optical techniques to measure hydration is growing due to the outcomes observed through the penetration of light at differing levels. This paper comprehensively reviews such measurement techniques, focusing on recent experimental studies and state-of-the-art devices.

Atrial fibrillation (AF) is one of the most common types of cardiac arrhythmia, with a prevalence of 1–2% in the community, increasing the risk of stroke and myocardial infarction. Early detection of AF, typically causing an irregular and abnormally fast heart rate, can help reduce the risk of strokes that are more common among older people. Intelligent models capable of automatic detection of AF in its earliest possible stages can improve the early diagnosis and treatment. Luckily, this can be made possible with the information about the heart's rhythm and electrical activity provided through electrocardiogram (ECG) and the decision-making machine learning-based autonomous models. In addition, AF has a direct impact on the skin hydration level and, hence, can be used as a measure for detection. In this paper, we present an independent review along with a comparative analysis of the state-of-the-art techniques proposed for AF detection using ECG and skin hydration levels. This paper also highlights the effects of AF on skin hydration level that is missing in most of the previous studies.

Background: Previous studies have demonstrated the possibility of adverse effects of prolonged wearing of personal protective equipment in healthcare workers. However, there are a few studies about the effects on skin characteristics after wearing a mask for non‐healthcare workers. In this study, we evaluated the dermatologic effects of wearing a mask on the skin over time. Materials and Method: Twenty‐one healthy men and women participated in the study. All participants wore masks for 6 hours consecutively. Three measurements were taken (a) before wearing the mask, (b) after wearing the mask for 1 hour, and (c) after wearing the mask for 6 hours. Skin temperature, skin redness, sebum secretion, skin hydration, trans‐epidermal water loss, and skin elasticity were measured. Results: The skin temperature, redness, hydration, and sebum secretion were changed significantly after 1 and 6 hours of wearing a mask. Skin temperature, redness, and hydration showed significant differences between the mask‐wearing area and the non–mask‐wearing area. Conclusion: Mask‐wearing conditions and time can change several skin characteristics. In particular, it is revealed that the perioral area could be most affected. [ABSTRACT FROM AUTHOR]

Systemic cryotherapy is a popular treatment involving a short stay in a cryogenic chamber at a temperature below −100 °C. This leads to a number of physiological reactions, some of them also observed in the skin. The aim of the study was to analyze the effect of a single cryogenic treatment on selected skin characteristics (skin pH, level of hydration and TEWL – Transepidermal Water Loss) in young, healthy people. Skin characteristics in 77 young people (23.63 ± 1.36 years) were assessed. In the study, 43 women and 33 men who took part in a one-time treatment (−120 °C) lasting 3 min. Measurements were made on the forearm skin and (in men) on the face twice: before and immediately after the procedure. Results: Initial differences in hydration of the stratum corneum and TEWL were observed between the group of women and men. After one treatment, the examined characteristics of the forearm skin did not change, and an unfavorable increase in TEWL in men was indicated in the facial area. A single stay in the cryogenic chamber, while maintaining the correct methodology of the treatment, is safe for the skin. The changes taking place depend on the body surface area tested, which indicates that the skin on the limbs and on the face reacts differently to the cryogenic stimulus. [ABSTRACT FROM AUTHOR]

Julie M Bianchini,1 Qihong Zhang,2 Gabriel Hanna,2 Carol R Flach,2 Hequn Wang,1 Michael D Southall,1 Richard Mendelsohn,2 Manpreet Randhawa11Johnson and Johnson Consumer Companies, Inc., Skillman, NJ, USA; 2Department of Chemistry, Rutgers University, Newark, NJ, USAIntroduction: As skin ages, it loses its ability to retain moisture and becomes rough and dry. This results in a clinically dull appearance with a loss of radiance, firmness, and suppleness. Symptoms can be improved with use of a moisturizer that builds and maintains skin hydration over time; however, most moisturizers that occlude the skin surface are perceived as heavy and greasy and are not consumer preferred.Methods: A unique, consumer-preferred gel matrix formula was developed by combining liquid crystal structures, which mimic skin barrier lipid assembly, with specific emulsifiers that deliver water deep into skin. Ex vivo studies were conducted to investigate the superior hydrating effects of the gel matrix formula. Confocal Raman microscopy studies assessed the spatial distribution of water in ex vivo skin after application of the gel matrix formula. To determine the effects of the gel matrix formula on dry facial skin, a 12-week clinical study was conducted with subjects with self-perceived skin dryness and dullness.Results: The formulation significantly increased the relative water content throughout epidermal regions, which was not observed with the application of a competitive gel formula. Instrumental measurements assessed improvements in skin surface moisturization and barrier function. Clinical grading showed significant improvements in hydration-related endpoints including radiance, clarity, and texture. Subject self-agree assessment demonstrated that subjects observed improvements in the appearance of their facial skin.Conclusion: These studies demonstrated that the gel matrix formula increased skin water content in deeper layers, and resulted in significant clinical improvements in hydration, barrier function, and clinical appearance of radiance.Keywords: confocal Raman microscopy, skin radiance, moisturization, dynamic skin barrier, skin hydration

Sea buckthorn (SB) (Elaeagnus rhamnoides (L.) A. Nelson) is rich in flavonoids, phenolic compounds, anthocyanins, carotenoids, and phytosterol. Its phytochemicals exhibit various biological activities, such as antioxidant, immunomodulatory and anti-carcinogenic activities. SB also helps prevent the development of wrinkles and protects the skin’s surface from UV rays. The purpose of the present study was to develop and characterize an SB oil (SBO)-loaded nanostructured lipid carrier (NLC) for improved skin hydration. The response surface methodology (RSM) and central composite design (CCD) were employed to optimize the influencing factors (wax percentage, surfactant percentage, and PEG400 percentage in the surfactant) to achieve the desirable qualities in SBO-NLCs. The optimum (minimum) size of SBO-NLCs (105.26 nm) was obtained with a combination of 2.5% wax, 7.5% surfactant, and 30% PEG400 in the surfactant. A narrow polydispersity index (PDI; 0.16), relatively low zeta potential (ZP; −15.63 mV), and high entrapment efficiency (EE; 90.88%) were observed in this study. Reduced quadratic and reduced 2FI models were adapted to predict conditions to attain the optimum size and PDI of SBO-NLCs, respectively. ZP and EE were predicted with the help of a reduced cubic model. All of the predicted models were statistically significant. Differential scanning calorimetry results suggested that the SBO-NLCs had less crystallinity and therefore reduced the rate of drug expulsion from the inner core of the NLCs. A noticeable level of occlusion effect was observed in the SBO-NLCs. The SBO-NLCs showed a faster vitamin E (biomarker for the drug) release rate into the skin within 24 h, and the released vitamin E level after 48 h was significantly higher than that for the free SBO. Additionally, SBO-NLCs delivered vitamin E into the inner skin significantly (22.73 ± 1.67 µg/cm2 of skin). In conclusion, the SBO-NLC is a potential delivery system that can be used to prevent skin water loss and improve skin hydration. Further investigations, such as drug stability and safety evaluations, are required prior to commercialization for human use.

Background: Handwashing using soap or hand sanitizer is one of the health protocols that must be implemented by society to prevent the spreading of SARS-COV2 virus which can cause pneumonia, acute respiratory syndrome and death. The implementation of health protocols during the COVID-19 pandemic caused the habit of handwashing increase. Hence, it can lead to cause irritant contact dermatitis and dry hand skin due to frequent contact with soap or hand sanitizers. Method: This study is an observational study with the sample from the villager of Banyumas regency. The data collection was taken by cluster random sampling and accidental sampling. Results: Considering the health protocol, most of the villager used to wash their hands regularly and 3 respondents (3.00%) of them did not. 100 respondents took the data and 19 respondents (19.00%) have irritant contact dermatitis and the rest did not. Furthermore, for skin hydration levels with dehydration category (0% - 29%) are 50 respondents (50.00%), normal category (30% - 50%) are 38 respondents (38.00%) and hydration category (51% - 100 %) are 12 respondents (12.00%). Chi-Square analysis showed that there was no significant relationship between handwashing habit and the incidence of irritant contact dermatitis with the value of “P” is 0.394> 0.05 and there was a significant relationship between hand washing habits and skin hydration levels with the value of “P” is 0.010

Background: The human skin is greatly affected by external factors such as UV radiation (UVR), ambient temperature (T), and air humidity. These factors oscillate during the year giving rise to the seasonal variations in the skin properties. The aim of this study was to evaluate the effect of seasons, environmental T, relative and absolute humidity on the skin parameters of Caucasian women, perform a literature review and discuss the possible factors lying behind the found changes. Materials and Methods: We measured stratum corneum (SC) hydration, transepidermal water loss (TEWL), sebum level, erythema index, and elasticity parameters R2 and R7 on the forehead and the cheek of Caucasian women from the Czech Republic throughout the year. We also performed a non‐systematic literature review focused on the seasonal variations in these skin parameters. Results: We confirmed a well‐documented low SC hydration and sebum production in winter. In spring, we found the lowest TEWL (on the forehead) and the highest SC hydration but also the highest erythema index and the lowest elasticity presumably indicating skin photodamage. For most of the skin parameters, the seasonal variations probably arise due to a complex action of different factors as we extensively discussed. Conclusion: The data about the seasonal variations in the skin parameters are still highly inconsistent and further studies are needed for better understanding of the normal skin changes throughout the year. [ABSTRACT FROM AUTHOR]

Background: Silicon is an abundant element in the human body and plays an important role in the skin, taking part in the synthesis of collagen and glycosaminoglycans. The use of nanotechnology methods, which processes materials at an atomic and molecular scale, has allowed the development of nanosilicons. Aims: The study evaluates the effectiveness of a food supplement and a topical solution containing nanosilicon in improving skin hydration and elasticity. Methods: A total of 30 female subjects were randomized to receive the placebo (n = 15) and the active compound (n = 15). All enrolled subjects took the food supplement twice a day for 20 days and then once a day for the next 20 days, and they also had to apply the nanosilicon solution on the right forearm four times a day. Evaluation of several parameters was performed after 20 and 40 days through the use of non‐invasive instrumental methods (Corneometer® CM 825, Cutometer® MPA 580, Visioscan® VC, Tewameter® TM 200). Results: Both treatment groups showed a statistically significant improvement in barrier function and in skin hydration on the right forearm after 20 days; increase in skin elasticity was observed only in the group taking the active compound. Conclusions: The study showed that the administration of a food supplement and a topical solution, both containing nanosilicon, improves hydration, elasticity, and skin barrier function. [ABSTRACT FROM AUTHOR]

Background Workers in the healthcare sector are at high risk of developing occupational hand eczema mainly due to frequent exposure to irritants and/or allergens. Amongst workers in healthcare, nurses are at higher risk of developing hand dermatitis. Objectives To evaluate the effectiveness of a short educational intervention program in preventing occupational hand eczema in nurse apprentices, using two objective tools, namely TEWL and EH, and the HECSI score. Methods Data regarding professions, wet work exposure, activities performed during working hours, self-reported eczema were collected from 230 nurse students, divided in two study groups: the intervention and the control group (CG). The intervention group (IG) was given education about risks and proper skin care and was provided with cosmeceuticals to be used for skin care during hospital activity. The evaluation of skin properties was performed using questionnaires, HECSI score, measurement of transepidermal water loss (TEWL) and epidermal hydration (EH). Results A number of 139 apprentice nurses completed the study. Of those participants who completed the study, 19.1% from CG and 19.6% from IG reported, at T1, hand eczema in the last 3 months, while at T2 (3 months later), 59.52 % of the CG and only 11.34 % from the IG stated having eczema in the last 3 months. In the IG, results showed an improvement of CM with 17% and of TEWL with 16%, with only a 0.5% improvement of CM in CG and a marked impairment of TEWL by 33%. Conclusion Hand eczema is a common occupational dermatosis affecting the medical staff, even during apprenticeship. Early preventive training programs are effective in reducing the burden of occupational contact dermatitis. [ABSTRACT FROM AUTHOR]

Background: Objective evaluation of skin condition on the basis of noninvasive methods has become obligatory and may be a good strategy for verifying the efficiency of new cosmetic active ingredients. Aim: The aim of this study was to assess the influence of aucubin‐containing cosmetic macroemulsion on skin parameters using the skin testing equipment. Patients/Methods: The study was carried out on the group of 25 female volunteers of the mean age 27 ± 2 years. The skin parameters were measured using the following devices: Tewameter® TM 300 (transepidermal water loss, TEWL), Corneometer® CM 825 (skin hydration), Cutometer® MPA 580 (skin elasticity), Visioscan® VC 98 (skin topography), and Visioline® VL 650 (skin macrorelief). All measurements were performed on the inner part of the left forearm. Results: The skin parameters measured revealed the beneficial influence of aucubin on TEWL and skin hydration level. The application of aucubin‐enriched cream caused more significant improvements of all determined SELS parameters, in comparison with cream without it. An analogous tendency was noticed in the case of skin macrorelief parameters; the addiction of the active ingredient led to a decrease in the value of total wrinkle area and diminished the mean length and depth of single wrinkles. Conclusions: Noninvasive methods of skin testing have provided a possibility of objective evaluation of the action of new active ingredients in cosmetic formulations. The study has proved the positive influence of aucubin on effectiveness of cosmetic macroemulsions, diminishing TEWL, increasing skin hydration level, and improving the values of SELS and skin macrorelief parameters. [ABSTRACT FROM AUTHOR]

Coffee bean chlorogenic acids, the major components of coffee polyphenols, have various physiological effects in humans such as anti-obesity, anti-hypertension and skin hydration when continuously ingested. Recently, it was reported that a single ingestion of coffee bean chlorogenic acids (CGAs) extracted from green coffee beans improved skin blood flow (SkBF) recovery after cold stress. On the other hand, it was observed that increases in skin hydration and transepidermal water loss (TEWL) were accompanied by a transient increase of SkBF. Thus, a single ingestion of CGAs is also expected to increase skin hydration. In this study, we investigated the effects of a single ingestion of CGAs on facial skin properties. A randomized, placebo-controlled, crossover intervention study was conducted in healthy adult women. Skin hydration, TEWL and SkBF were measured on the cheeks of subjects before and at 60 and 120 min after the ingestion of 270mg CGAs or a placebo with a 1-week washout period. Twenty subjects were enrolled and all completed the study. Significant increases of skin hydration, TEWL and SkBF were observed in the CGAs group at 60 and 120 min compared with the placebo group. These findings suggest that a single ingestion of CGAs transiently increases skin hydration, TEWL and SkBF. [ABSTRACT FROM AUTHOR]

Skin water content monitoring is important for diagnostics and management of edema, dehydration, and other skin conditions as well as for cosmetic applications. Because optoacoustic (OA) technique has high (optical) contrast and (ultrasound) resolution and significant probing depth, it may be suitable for accurate, noninvasive water content monitoring in the skin. In this work we studied OA response from skin tissue phantoms and human wrist skin in the wavelength range from 1370 nm to 1650 nm using a novel, tunable OPO OA system. We identified optimal wavelengths for OA water content monitoring in different skin layers. The results of our study suggest that the OA technique may become a valuable, quantitative tool for accurate, high‐resolution water content monitoring in the skin and other tissues and may find wide applications in dermatology, cosmetology, and tissue trauma management. [ABSTRACT FROM AUTHOR]

Introduction: Although collagen is widely used in various forms as a functional ingredient in skin care products, the effect of oral supplementation of collagen tripeptides (CTPs) on human skin is unclear. Moreover, the majority of the positive outcomes of CTP reported so far have not considered the effect of weather conditions. Therefore, we tested the effect of CTP and adjusting for climate change on skin properties in middle-aged women.Materials and Methods: A randomized controlled trial was conducted with 84 women between 40 and 60 years of age. Participants were randomized to receive placebo or 1,000 mg CTP daily for 12 weeks. CTP was prepared from the skin of Nile Tilapia by the digestion method using collagenase from non-pathogenic bacteria of the genus Bacillus. Skin hydration, wrinkling, and elasticity were assessed at baseline and after 6 and 12 weeks with adjustments for temperature, humidity, and ultraviolet A exposure during the evaluation time using weather data from the regional meteorological office.Results: Of the 82 participants, 74 completed the trial without adverse effects. Compared with the control group, trans-epidermal water loss was reduced more in the CTP group after 12 weeks (P < 0.05). At 12 weeks, even after adjustment for humidity, temperature, and UVA in the region, the difference of the two groups in TEWL remained statistically significant (adjusted for humidity and temperature, P = 0.024; adjusted for UVA, P = 0.032; adjusted for temperature, high temperature, and ultraviolet A, P = 0.031). In terms of skin hydration, more improvement was evident in the CTP group than in the control group. In the subgroup analysis, subjects under 50 years of age showed a significant improvement in total score and moisture in the subjective skin improvement questionnaire after taking CTP for 12 weeks. Application of CTP was well-tolerated, and no notable adverse effect was reported from both groups.Discussion: Our findings suggest that oral ingestion of CTP from the Skin of Nile Tilapia (Oreochromis niloticus) is well-tolerated and helps reduce water loss in in middle-aged women.Clinical Trial Registration:www.clinicaltrials.gov/, Identifier: NCT03505684.

Purpose: Five different galenics were analyzed and compared concerning tissue breathability and gas exchange with the environment after an application period of 6 h on pig ear skin. Aim was to find the most suitable galenics for efficient moist treatment for everyday injuries (abrasions, lacerations and cuts) without influencing the transepidermal water loss. Methods: A quantity of 0.1 g of the different test preparations was applied once topically to an area of 2 cm2. The analysis of the breathability was performed by TEWL (transepidermal water loss) measurements in the first hour after product application. The moisture retention effect was assessed by corneometry in the first 5 h after product application. Results: The hydrogel preparations showed a higher breathability in contrast to a semi-occlusive ointment and petrolatum. The same applies to the moisture penetration of the skin. Here, all hydrogel formulations showed the highest tissue hydration. After 3 h an additional increase in moisture was observed for the areas treated with Tyrosur® CareExpert Wound Gel and the ointment. Conclusion: In contrast to petrolatum and the semi-occlusive ointment, treatment with the hydrogels led to a preservation of the breathability and good moistening of the tissue, which is due to the galenics of the gels consisting of water, carbomer and propylene glycol. The increase in moisture after 3 h in areas treated with Tyrosur® CareExpert Wound Gel and the semi-occlusive ointment indicates a sustained moisturizing effect mediated by dexpanthenol.

(1) Background: The study systematically investigated the influence of dispersed particles within a topical formulation on the dermal penetration efficacy of active compounds that are dissolved in the water phase of this formulation. The aim was to prove or disprove if particle-assisted dermal penetration can be used for improved dermal drug delivery. (2) Methods: Fluorescein was used as a surrogate for a hydrophilic active ingredient (AI). It was dissolved in the water phase of different formulations with and without particles. Two different types of particles (titanium dioxide and nanostructured lipid carriers (NLC)) were used. The influence of particle size and number of particles and the influence of skin hydrating excipients was also investigated. (3) Results demonstrate that the addition of particles can strongly increase the dermal penetration efficacy of AI. The effect depends on the size of the particles and the number of particles in the formulation, where smaller sizes and higher numbers resulted in higher penetration parameters. Formulations with NLC that contained 20% w/w or 40% w/w particles resulted in an about 2-fold higher amount of penetrated AI and increased the penetration depth about 2.5-fold. The penetration-enhancing effect was highly significant (p < 0.001) and allowed for an efficient delivery of the AI in the viable dermis. In contrast, the penetration-enhancing effect of excipients that increase the skin hydration was found to be very limited and not significant (≤5%, p > 0.05). (4) Conclusions: Based on the results, it can be concluded that particle-assisted dermal penetration can be considered to be a simple but highly efficient and industrially feasible formulation principle for improved and tailor-made dermal drug delivery of active compounds.