Search

UMR AGAP Equipe DAAV Diversité, adaptation et amélioration de la vigne; Grape varieties and rosé wines, a database resulted of 15 years of experimental vinifications. 19. Journées Internationales de Viticulture GiESCO

WetVegEurope is a research project (http://www.sci.muni.cz/botany/vegsci/wetveg) whose goal is to provide a synthesized formalized classification of the aquatic and marsh vegetation across Europe at the level of phytosociological associations. In order to achieve the project objective, a WetVegEurope database has been created (GIVD ID: EU-00-020, http://www.givd.info/ID/EU-00-020), which currently contains 375,212 vegetation plots of aquatic, marsh and wet vegetation types from 33 European countries. The WetVegEurope database includes datasets from pre-existing national and thematic databases and also 10,616 plots previously not digitalized or even unpublished. This database offers an extensive source of data for future studies on aquatic and marsh plant species and vegetation types at the European scale.

The online open access edition of the Hesperia databank is a milestone of great significance in the study of Paleohispanic languages. In this paper are revised the characteristics of this online edition, by examining the differences involved in online publication based on the possibilities of hypertext, compared to print edition., La publicación en abierto de la base de datos Hesperia supone un hito de gran relevancia en el estudio de las lenguas paleohispánicas. En esta contribución se repasan las características de esta edición en línea, examinando las diferencias que supone la publicación en línea, basada en las posibilidades del hipertexto, frente a la publicación del texto impreso.

Submitted by Isabel Gomes (itg@lisboa.ucp.pt) on 2018-11-27T14:41:41Z No. of bitstreams: 1 152112099 Laura Tepner W.pdf: 5274497 bytes, checksum: e20b1d172c400bf3ec7f063be56c1da2 (MD5) Approved for entry into archive by Isabel Gomes (itg@lisboa.ucp.pt) on 2018-11-27T14:42:00Z (GMT) No. of bitstreams: 1 152112099 Laura Tepner W.pdf: 5274497 bytes, checksum: e20b1d172c400bf3ec7f063be56c1da2 (MD5) Made available in DSpace on 2018-11-27T14:42:00Z (GMT). No. of bitstreams: 1 152112099 Laura Tepner W.pdf: 5274497 bytes, checksum: e20b1d172c400bf3ec7f063be56c1da2 (MD5) Previous issue date: 2014-11-06

In het kader van de eerste fase van het onderzoeksprogramma inzake geologische opberging van radioactief afval is een inventarisatie en evaluatie van geohydrologische gegevens uitgevoerd. Technieken die bruikbaar zijn om een ruimtelijk beeld van de ondergrond te reconstrueren zijn beschreven en toegepast (bijv. de methode van reservoir-analyse). Het rapport geeft voor de geologische eenheden in NO en O Nederland een inventarisatie van de beschikbare openbare gegevens. Gegevens van Tertiaire lagen zijn opgeslagen in een geautomatiseerd systeem waardoor grafische presentaties mogelijk worden. Met behulp van de gegevens en de reservoir-analyses zijn geohydrologische profielen samengesteld door de door de Rijks Geologische Dienst beschreven zoutstructuren. Deze profielen bevatten de gegevens die in het project veiligheidsevaluatie worden gebruikt.

In het kader van de eerste fase van het onderzoeksprogramma inzake geologische opberging van radioactief afval is een inventarisatie en evaluatie van geohydrologische gegevens uitgevoerd. Technieken die bruikbaar zijn om een ruimtelijk beeld van de ondergrond te reconstrueren zijn beschreven en toegepast (bijv. de methode van reservoir-analyse). Het rapport geeft voor de geologische eenheden in NO en O Nederland een inventarisatie van de beschikbare openbare gegevens. Gegevens van Tertiaire lagen zijn opgeslagen in een geautomatiseerd systeem waardoor grafische presentaties mogelijk worden. Met behulp van de gegevens en de reservoir-analyses zijn geohydrologische profielen samengesteld door de door de Rijks Geologische Dienst beschreven zoutstructuren. Deze profielen bevatten de gegevens die in het project veiligheidsevaluatie worden gebruikt.

The governance of knowledge seems to be the Scientific Policy most able to creating value with regard of human and its evolution in cultures and civilizations. The duty of good governance is a consideration of the transfer of knowledge related to scientific and technological progress. Intrinsically, this process requires a system of organization and knowledge management by implementing knowledge production and its influence in society. The objective of the ISKO-Maghreb Chapter is to contribute in understanding the factors that organize knowledge and phenomena that affect the information society. Actions to be undertaken by the scientific ISKO society must take into account socio-cultural, cognitive and economic in the strategic management of knowledge. Towards the knowledge society, it should be seen in its dynamic, its content and its interaction with science and technology associated to universities, companies and politics. In this context, a first orientation is pedagogical attempt to answer the question "what is known about the knowledge and its organization?". Then the issue is moving towards the societal issues of knowledge, theory and practice, to provide clarification to a convergence of KM (Knowledge Management) approaches. Education, science, culture, communication and technology remain the major themes covered by ISKO-Maghreb, for the development of the knowledge organization, expertise management and collective intelligence. In a friendly atmosphere, hospitality and open to exchange, the international symposium ISKOMaghreb' 2012 was thought to enhance the Scientific Society "ISKO" with the universities, the practitioners in the Maghreb countries and the world., La gouvernance de la connaissance paraît être la politique scientifique la plus apte à la création de la valeur au regard de l'Homme et de son évolution dans les cultures et civilisations. Le devoir de cette gouvernance est une prise en considération de la transmission des savoirs liée aux progrès scientifiques et technologiques. Intrinsèquement, ce processus de transmission nécessite un système d'organisation et de management de la connaissance mettant en oeuvre la production du savoir et son rayonnement dans la société. L'objectif du chapitre ISKO-Maghreb est de contribuer dans la compréhension des facteurs qui organisent la connaissance et les phénomènes qui affectent la société de l'information. Les actions à entreprendre par la société savante ISKO devront tenir compte des aspects socio-culturels, cognitifs et économiques dans la gestion stratégique de la connaissance. Vers la société du savoir, la connaissance doit être perçue dans sa dynamique, sa teneur et ses interactions scientifiques et technologiques avec les universités, les entreprises et les politiques. Dans ce contexte, une première orientation est d'ordre pédagogique pour tenter de répondre à la question "que sait-on de la connaissance et de son organisation ?". Ensuite, la question évolue vers les enjeux sociétaux du savoir, en théorie et en pratique, pour apporter des clarifications vers une convergence des démarches KM (Knowledge Management). Éducation, science, culture, communication et technologie restent les thématiques majeures couvertes par ISKO-Maghreb, pour la mise en place de l'organisation de la connaissance, de la gestion des compétences et de l'intelligence collective. Dans un cadre convivial, chaleureux et ouvert aux échanges, le colloque international ISKO-Maghreb'2012 a été pensé en vue de renforcer la Société savante " ISKO " avec les universitaires, les praticiens dans les pays du Maghreb et le reste du monde.

Facebook compte 500 millions d’inscrits. Au premier regard, exposition de la vie, le réseau social montre la prégnance de la mort. Trois genres d’annonces sont analysés. Les faire-part : un membre annonce la mort d’un proche ; les malaises dans la communication : quelqu’un est mort, sa page existe encore, et les commémorations consécutives à des catastrophes, à des accidents, à des crimes, à des maladies. L’étude rend compte des débats sur le statut des données numériques des morts, enjeu affectif et symbolique. Elle s’attache au rôle des mémoriaux, qui montrent à l’œuvre une morale des réseaux sociaux, la sensibilité et la responsabilité. En devenant un cimetière global, Facebook se constitue comme un lieu de socialisation numérique auquel personne ne peut se soustraire. De ce fait, les morts – sous leur forme numérique ou par leurs banques de données – constituent une force d’influence dont on peut se demander comment elle sera orientée. Three situations are analysed: Death announcement of a close relative, Issues caused by dead members’ pages, “Memorial” pages. The intimate mourning is not easy to express. A “know-how” is developing. A moral code (of solidarity and responsibility) is progressing. Real digital cemeteries are constituting, imposing social networks as places of socialization. Dead people become in a way an influence group.

Las fuentes de información legislativa en materia de telecomunicaciones se encuentran dispersas por cada una de las entidades, lo que produce una desorganización e impide conocer la vigencia de las normas y así mismo su debida aplicación. Es por esto que surge la necesidad de conformar un Banco de Datos Jurídico Automatizado sobre Interconexión de Redes de Telecomunicaciones que haga parte del Banco de Datos sobre Regulación en Telecomunicaciones, de tal manera que sirva como herramienta de desarrollo académico, y acerque a la Universidad a las entidades regulatorias, para proveer soluciones a la desorganización legislativa del sector. Este trabajo de grado hace una explicación de la metodología llevada a cabo para el análisis de la información jurídica, explicando las necesidades que llevan a la conformación del banco de datos y los beneficios que trae la integración de la Informática y el Derecho. Adicionalmente se muestra como fue el desarrollo del tema particular de Interconexión de redes de proveedores de redes y servicios de telecomunicaciones, haciendo énfasis en las dificultades e inconvenientes a lo largo del trabajo. Finalmente se muestran los resultados de la crítica estructural y el Tesauro Temático Especializado para ser incluido en el sistema. The legislative information sources about telecommunications are scattered byeach one of the entities, resulting disorganization, prevents know the validity of norms and their appropriate application. For that reason there is the need to establish an AutomatedJuridical Data Bank on Interconnection of telecommunications networks, as a part of the Data Bank onRegulation in Telecommunicationsin such a way that serves as a tool for academic development, and put together University with Regulatory Agencies, solving the legislative disorganization in the sector. This grade work makes anexplanation of the methodology used for analyzing the juridical information, explaining the needs that lead to the creation of the database and the benefits that brings the integration of Information and Law. Additionally it shown as was the development of the particular issue of interconnection networks of providers of telecommunications networks, emphasizing the difficulties and disadvantages throughout the work. Finally it shows the results of the structural critiqueand The Specialized Thematic Thesaurus to be included in the system. Ingeniero de Telecomunicaciones Pregrado

El proyecto tiene como finalidad la contribución de un banco de datos en Regulación de telecomunicaciones sobre el espectro radioeléctrico, con el fin de favorecer con el desarrollo académico de los estudiantes de Pregrado, Especialización, Diplomado y profesores de la Universidad Santo Tomás. Para el desarrollo de este proyecto, se requirió realizar la organización y análisis de la vigencia de la normatividad para la elaboración de los descriptores y continuamente realizar la conformación de la estructura temática especializada. Finalmente, se realizó la presentación formal del Tesauro Temático Especializado en una incorporación al sistema de los documentos normativos. The purpose of the project is to contribute to a databank on Regulation of Telecommunications regarding the radio-electric spectrum, in order to stimulate the academic development of students’ of the undergraduate, specialization, diploma and faculty at St. Thomas University. The development of this project required the arrangement and analysis of the validity of the regulations, for the development of the descriptors and to continuously carry out the formation of the specialized thematic structure. Finally, there was the formal presentation of the Specialized Thematic Thesaurus, as an addition to the system of normative documents. Ingeniero de Telecomunicaciones Pregrado

VegItaly (http://www.vegitaly.it; GIVD ID EU-IT-001) is a web database designed to store, retrieve and analyse the vegetation data of Italy. Designed for both floristic and phytosociological data, it can handle any field plot according to the definition of “vegetation database” suggested by the Global Index of Vegetation-Plot Databases (GIVD) and the IAVS Ecoinformatics Working Group. VegItaly, supported by Societa Italiana di Scienza della Vegetazione (formerly Societa Italiana di Fitosociologia), is hosted by AnArchive (http://www.anarchive.it), a collaborative project aimed at archiving botanical data that involves several Italian universities coordinated by the University of Perugia. VegItaly’s main aim is to contribute to data archiving and sharing in order to offer the possibility to manage large data sets for statistical analysis on a wide geographic scale.

A terminológiai adatbázisokat napjainkban a hatékony fordítói segédeszközök között tartjuk számon. De vajon milyen mélységig ismerjük ezeket a segédeszközöket, mennyire hasznosítjuk a bennük rejlő lehetőségeket? A tanulmány szerzője konkrét példák elemzésével kísérletet tesz a terminológiai adatbázisok hármas felosztására. A megjelenítési felület alapján megvizsgálja felépítésüket, az adatmezők típusait, az általuk tartalmazott adatmennyiséget és az egyéb kapcsolódó elemeket, majd ismerteti az egyszerű, a hagyományos és az összetett terminológiai adatbázisok alapvető jellemzőit. A terminológiai adatbázisok szerkesztése egyfelől megkívánja, hogy ismerjük a terminológia elveit és módszereit, másfelől szükséges elrugaszkodni a hagyományos, nyomtatott segédeszközökre jellemző szemlélettől és élni a szoftverek kínálta lehetőségekkel. A tanulmány kitér a terminológus összetett feladataira is, amelyeket a modern szemléletet és igényeket követve mutat be.

Introduction: The risk of cancer has been extensively investigated with the intake of single food items but food consumption in an individual is usually complex, made up by combinations of different food items. This study aims to identify dietary patterns of Malaysians, and establishing relationship between dietary pattern and oral cancer risk. Methods A hospital-based, case-control study was conducted on 300 Malaysians who seek treatment at participating hospitals between January 2003 and December 2010. The subjects consisted of 150 cases and 150 controls that were matched for gender, age (5 years) and ethnicity. Dietary intake was measured using Food Frequency Questionnaire (FFQ). Factor Analysis (FA) was performed to identify dietary consumptions pattern based on the intake of nine major food groups. Logistic Regression was done to compute the odds ratio (OR) for the components retained by FA. Results: FA had identified four patterns that accounted for 69.4% of total variability within the sample. The first pattern labeled as 'modern' was loaded with processed foods and snacks intakes whereas the second pattern 'prudent' was characterized by the intakes of fruits and vegetables. The third pattern 'traditional' consisted of beverages and starches intakes while the fourth pattern 'combination' was loaded with intakes of dairy foods, fermented foods and meat/byproducts. A significant reduced risk was found for 'prudent' (aOR 0.42, 95%CI: 0.21–0.83, p < 0.05), whereas a significant increased risk was found for both 'traditional' (aOR 2.06, 95%CI: 1.05–4.02) and 'combination' (aOR 3.45, 95%CI: 1.75–6.77, p < 0.05) pattern. Discussion: These findings suggest that intake of 'prudent' pattern may confer protection against oral cancer risk of up to 58% whereas the highest consumption of both 'traditional' and 'combination' pattern may induce twice and thrice the risk of oral cancer. FA was also found to be a useful tool to identify dietary pattern and its relationship with oral cancer. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Introduction: Oral cancer is a debilitating disease and the survival rates for these patients have not improved over the past decades. Tobacco smoking is one of the most common risk factor associated with oral cancer. Objectives: To explore and identify differential genes expression associated with tobacco smoking. Methodology: Next generation sequencing using the Illumina Genome Analyzer was done to sequence five fresh frozen oral cancer tissue samples from smoking patients, two of which were former smoker and an additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients where 4 were smokers and another 4 were non-smokers. The tissues and socio- demographic information were obtained from the Malaysian Oral Cancer Data and Tumour Bank System (MOCDTBS) at the Oral Cancer Research and Coordinating Centre (OCRCC). High quality poly A+ RNA was extracted from macrodissected tumour and normal epithelial tissue to obtain >70. Validation of second generation sequencing was done using commercial and custom microarrays. Principle component analysis (PCA) was applied to the sequenced generated data. Results and discussion: PCA showed distinct clusters separating groups of current and former smokers. The former smoker who ceased smoking >25 years clustered closely with normal non-smoker suggesting expression levels of some of the genes related to tobacco smoking could return to levels similar to never smokers upon cessation of smoking. When comparing differential gene expression between tumor in former and current smokers, ACTC1, MYH2, DES, MYBPH, MYLPF were the top 5 most down-regulated genes. Meanwhile, CYP2W1, FAIM2, OLFML1, KRT13 were found to be up-regulated when comparing differential gene expression between these groups (fold change > 8). Despite prolonged smoking cessation, some gene expression could appear to be permanently altered and these irreversible changes may account for oral cancer risk despite smoking cessation. Conclusion: These findings illustrate the potential for next generation sequencing to provide insights into the unique gene expression profiles associated with risk habits which will be useful in developing biomarkers for prognostic and therapeutic applications in the future. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Introduction: HPV infection has been associated with a subset of head and neck cancers and current evidence suggest that it may be an important risk factor for oral cancer. Using polymerase chain reaction (PCR) and sequencing, we recently demonstrated the presence of HPV in more than 50 of oral squamous cell carcinoma (OSCC) patients and that high-risk HPV is significantly associated with OSCC. Serological detection of HPV has been reported the most convenient method for detecting HPV. However, currently there is a lack of serological assays for the detection of the HPV. The HPV E6 viral oncoprotein is known to play crucial role in tumorigenesis, therefore detecting the presence of the E6 protein could be a useful biomarker for HPV detection.Methods: A pGEX plasmid containing HPV 16 E6 gene was ligated with KT3 oligonucleotide. Constructed plasmid was then transformed into Escherichia coli for production of the recombinant protein which was used as antigen in ELISA assay. ELISA was optimized using anti KT3 antibody to detect the recombinant antigen. HPV ELISA was performed on sera from 18 healthy and 15 OSCC patients obtained from the Malaysian Oral Cancer Database & Tissue Bank System (MOCDTBS) which is coordinated by Oral Cancer Research & Coordinating Centre (OCRCC). Sera that have net OD above calculated cutoff value were determined as HPV seropositive. Fisher's Exact test was used for statistical evaluation.Results: An ELISA method to detect the presence of HPV16 E6 protein was successfully developed. Using this method, 33.3 (5/15) of OSCC and 16.7 (3/18) of healthy patients were found to be HPV 16 seropositive. No significant association was found between HPV 16 seropositivity and OSCC occurrence (P value = 0.428).Discussion: Although there is a trend to support our previous findings using PCR where a larger proportion of OSCC patients were HPV positive in comparison to healthy individuals, our results using ELISA method did not show any statistical significance. This remains to be tested in a larger sample set to confirm pur preliminary result that could more representative of our patient population. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Introduction: ITCs found in cruciferous vegetables has been reported to reduce cancer risk by inducing phase II conjugating enzymes, in particular GSTs. Interestingly, these enzymes also metabolize ITCs therefore; the protective effects of ITCs would depend on the activity of GSTs. This study aimed to determine association between dietary ITCs, GSTs polymorphisms and risk habits (cigarette smoking, alcohol drinking and betel-quid chewing) with oral cancer.Method: Included in this study were 115 OSCC patients and 116 healthy subjects. Information on ITC intake from cruciferous vegetables was collected via a semi-quantitative FFQ. Peripheral blood lymphocytes were obtained for genotyping of GSTM1, GSTT1 and GSTP1 using PCR multiplex and PCR-RFLP. Chi-square and logistic regression were performed to determine the association of ITC and GSTs polymorphism and risk of oral cancer.Results: When dietary ITC was categorized into high (greater than/equal to median) and low (less than median) intake, ITC consumption was higher among cases (51.3%) than controls. However, it was not statistically significant (p = 0.645). Discussion: Odd ratios analysis showed no significant association between ITC intake, GSTM1, GSTT1 or GSTP1 genotypes with oral cancer risk. However, GSTP1 wild-type was associated with later disease onset in women above 55 years of age (p = 0.017). Among men above 45 years of age, there was a significant 17-year difference in the age of OSCC onset between those with GSTP1 wild-type ? low ITC intake and GSTP1 polymorphism ? high ITC intake (p < 0.001). Similarly further analysis stratified by risk habits (drinking and chewing), showed that GSTP1 polymorphism ? high ITC intake was associated with earlier disease onset (p < 0.001). This study suggests that combinatory effects between dietary ITCs, GSTP1 polymorphism and risk habits may be associated with risk of oral cancer and modulate the age of disease onset. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Availability of and accessibility to accurate, reliable and timely data is germane to the operation of the property market, in the field of estate surveying and valuation either for valuation/appraisal, management and agency purposes. In this study, the researcher examines the factors militating against accessibility to such data. The study was conducted using questionnaires, administered on practicing Estate Surveyors and Valuers, within Lagos Metropolis. 190 questionnaire was retrieved out of 300 questionnaire (i.e. 63%) administered on the respondents selected from the Nigerian Institution of Estate Surveyors and Valuers’ Directory and this was used for the analysis. The study revealed that inaccessibility to reliable data is a major barrier to valuation consistency. The study further revealed that there is the need for members of the Nigerian Institution of Estate Surveyors and Valuers, just like their counterpart in RICS, UK, to join hands together and create a strong databank comparable to that of the Investment Property Databank (IPD). The databank created as such should therefore be available and accessible to anyone that requires such information, even at a token.

Purpose: The purpose of this study was to evaluate the role of HPV and p53 polymorphisms in oral squamous cell carcinomas (OSCC) affecting Malaysian population. Methods: We analysed frozen samples from 105 OSCC as well as 105 oral specimens derived from healthy individuals. PCR assays targeting two regions of the virus were used. PCR amplification for the analysis of p53 codon 72 arginine/proline alleles was carried out in a separate reaction. Results: HPV DNA was detected in 51.4% OSCC samples, while 24.8% controls were found to be HPV positive. HPV was found to be significantly associated with OSCC (P < 0.001, OR = 4.3 after adjustment for habits) when compared to controls. High-risk HPV was found to be significantly associated with OSCC cases (P < 0.05). Demographic profiles of age, gender, race and habits were not associated with HPV presence in cases and controls. However, significantly less HPV positivity was seen in poorly differentiated compared to well-differentiated OSCCs. No significant association was found between HPV positivity and p53 polymorphisms in cases and control groups. Additionally, we found no association of codon 72 polymorphism with oral cancer. Conclusions: This study indicates that high-risk HPV infection is one of the contributing factors for OSCCs. HPV 16 was the predominant type found in Malaysian patients with OSCC. Further, we did not find any association between p53 codon 72 polymorphism and HPV infection or between the p53 polymorphism and the risk of oral cancer. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Introduction: Several molecular markers have been studied for their usefulness as prognostic markers in oral squamous cell carcinoma (OSCC). One such molecular marker is cyclin D1 which is a proto-oncogene located on 11q13 in humans. Objective: To explore the feasibility of using cyclin D1 as a prognostic marker in tongue and cheek SCC by the fluorescent-in-situ hybridization (FISH) method. Methods: Fifty paraffin-embedded samples (25 each of cheek and tongue SCCs) were obtained from the archives of the Oral Pathology Diagnostic Laboratory. Sociodemographic data, histopathologic diagnoses, lymph node status and survival data were obtained from the Malaysian Oral Cancer Database and Tissue Bank System (MOCDTBS)coordinated by the Oral Cancer Research and Coordinating Centre (OCRCC), University of Malaya. The FISH technique was used to detect the amplification of cyclin D1 using the Vysis protocol. Statistical correlations of cyclin D1 with site and lymph node status were analyzed using the Fisher exact test. Kaplan-Meier and Log Rank (Mantel-Cox) test were used to analyze cyclin D1 amplification and median survival time. Results: Positive amplification of cyclin D1 was detected in 72 (36) of OSCCs. Detection of positive amplification for cyclin D1 was observed in 88 (22) and 56 (14) of the tongue and cheek tumors, respectively, where the difference was statistically significant (P=0.012). Lymph node metastasis of cheek SCCs showed a trend towards a significant association (P= 0.098) with cyclin D1 amplification whereas the lymph node metastasis of tongue SCC was clearly not significant (P=0.593).There was a statistically significant correlation between cyclin D1 positivity and survival rate (P=0.009) for overall SCC cases and (P

The implementation of information technology in healthcare is a significant focus for many nations around the world. However, information technology support for clinical care, research and education in oral medicine is currently poorly developed. This situation hampers our ability to transform oral medicine into a 'learning healthcare discipline' in which the divide between clinical practice and research is diminished and, ultimately, eliminated. This paper reviews the needs of and requirements for information technology support of oral medicine and proposes an agenda designed to meet those needs. For oral medicine, this agenda includes analyzing and reviewing current clinical and documentation practices, working toward progressively standardizing clinical data, and helping define requirements for oral medicine systems. IT professionals can contribute by conducting baseline studies about the use of electronic systems, helping develop controlled vocabularies and ontologies, and designing, implementing, and evaluating novel systems centered on the needs of clinicians, researchers and educators. Successfully advancing IT support for oral medicine will require close coordination and collaboration among oral medicine professionals, information technology professionals, system vendors, and funding agencies. If current barriers and obstacles are overcome, practice and research in oral medicine stand ready to derive significant benefits from the application of information technology. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Oxidative stress, i.e. excessive content of reactionary, oxygen, and nitrogen compounds (ROAC), including free radicals, is one of the causes of various dangerous diseases as well as premature aging. The adverse effect of free radicals can be neutralized by antioxidants. In order to carry out antioxidant therapy, one needs to know the contents of antioxidants in food products. We have created the databank for the contents of antioxidants in 1,140 food products, beverages, etc. Apart from water-soluble antioxidants, fat-soluble antioxidants in dairy and fish products, cacao, chocolate, nuts etc. were determined for the first time using an amperometric method.

Objectives: This study was done to investigate the role of single nucleotide polymorphisms(SNPs) within genes of phase I (CYP1A1) and phase II (GSTM1, GSTT1,GSTP1) of the xenobiotic metabolism and its association with oral cancer risk.Methods: An unmatched case-control study was conducted using 207 newly diagnosed oral cancer patients and 117 non-cancer subjects selected from the OCRCC database. Peripheral blood was obtained from consented individuals and the CYP1A1, GSTM1, GSTT1 and GSTP1 genotypes were determined using polymerase chain reaction (PCR) and restriction enzyme digestion (RFLP). Simple and multiple logistic regression yielding odds ratio (OR and aOR) were employed to measure the association between genetic polymorphisms and risk of oral cancer. Results: In comparing cases and controls for CYP1A1, GSTM1 and GSTT1 polymorphism, the OR was 0.84 (95 CI 0.534 - 1.330), 0.99 (95 CI 0.627 - 1.554) and 0.87 (95 CI 0.541 - 1.388) respectively. However, the adjusted OR for GSTP1 polymorphism, as compared to the wild-type, was 0.43 (95 CI 0.221 - 0.837). It was noted that polymorphism of GSTP1 conferred a 57 reduction in risk of oral cancer as compared to individuals with the GSTP1 wild type genotype. Meanwhile individuals with combination of betel quid chewing habit and/or GSTP1 polymorphism has 1.6 times the risk of oral cancer although it was not statistically significant (95 CI 0.974 - 2.635). Conclusions: Analysis suggested that polymorphism of GSTP1 seems to have protective effect on the risk of oral cancer. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Objectives: To characterize differential gene expression between oral cancerous tissues and the normal mucosal tissues of oral cancer patients and oral mucosal tissues of cancer free patients using second generation sequencing. Methods: Five fresh frozen oral cancer tissue samples from smoking patients, with 2 samples of proximal normal were included in this study. An additional 8 samples consisting of normal oral mucosal tissues from the alveolar mucosa of non-cancer patients who had their wisdom tooth removed where 4 were smokers and another 4 were non-smokers was also included. The tissues and socio-demographic information were obtained from the Malaysian Oral Cancer Data and Tumour Bank System (MOCDTBS) at the Oral Cancer Research and Coordinating Centre (OCRCC). Manual macrodissection was used to obtain >70% tumour or normal epithelial tissue. High quality poly A+ RNA was extracted from the macrodissected tissues and second generation sequencing was done using the Illumina Genome Analyzer. Validation of second generation sequencing was done using commercial and custom microarrays. Results: Among the differentially expressed genes, KRT76 which encodes a filament protein that is responsible for the structural integrity of epithelial cells was the most down-regulated gene when comparing all tumours to all normals (excluding proximal normals). KRT76 is the second most down-regulated gene when comparing differential expression between proximal normal and normal non-cancer samples. Further comparison of all tumors against all normals, including the proximal normals, still indicates down-regulation of KRT76, but to a much lesser degree. A similar pattern was also observed for other down-regulated genes such as the KRT2, KRT3, ANKS1B, CPLX2, FGB, HIST1H3A, HIST1H3G, HIST1H4I, LOR and ZFHX2.Conclusions: This pattern of partial cancerisation of proximal normal samples issuggestive of the possible presence of _field cancerisation'.Relevance: The ''field cancerisation'' theory was postulated by Slaughter whereby the occurrence of multiple independent primary tumours was explained by the fact that the upper aerodigestive tract is chronically exposed to carcinogens. Various conventional molecular methods including microarray based technologies have been applied. Nevertheless, there have been no reports utilizing second generation sequencing to elucidate differentially expressed genes in relation to _field cancerisation' in oral carcinogenesis. Using second generation sequencing to discover genes involved in _field cancerisation' may be useful in predicting the outcome of different management strategies for oral cancer patients. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Background: Application of PCR techniques requiring only minute amounts of Genomic DNA. Thus, a less invasive, simpler to perform, and cheaper method to obtain DNA from exfoliated cells is desirable. We aim to develop a method that can obtain high quality of genomic DNA from one sample that allows for numerous application of PCR analysis. Objective: This study describes a simple, inexpensive and non invasive protocol to isolate a high quality of genomic DNA from exfoliated cells by using swish method. Methodology: Twenty two subjects vigorously swished 10 ml of normal saline in their mouth for 60 s and spitted into a collection tube. DNA extraction assay was performed by using saliva DNA isolation kit (Norgen, USA). The washed pellets were suspended in TE buffer and analyzed for the quality and purity of DNA content by using the NanoDrop Spectrophotometer. A ratio of A260/A280 was calculated. The extracted genomic DNA was amplified with primers of p53 intron 6 by using PCR machine. The presence of amplified DNA was then confirmed by electrophoreses analysis, which DNA bands were scanned by Typhoon 9410 variable imager. Results: In this study, the extracted genomic DNA demonstrated an average value of 1.94 O.D. in DNA content purity and 42.9 \backslashensuremath\ensuremathμg/\backslashensuremath\ensuremathμl in DNA yields. The electrophoresis images of the DNA products showed visible and detectable bands of higher molecular weight DNA in all the samples. Conclusion: The results showed that the extracted genomic DNA from the exfoliated cells by applying the swish method, that provides substantially larger amounts and higher molecular weight of DNA for down-stream DNA identification application. In addition, all samples were successfully genotyped by PCR-based assays for p53 gene intron 6 regions, which confirmed that the quality of isolated DNA was reliable in supporting the PCR amplification for the molecular studies. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Lithica is een databank, opgebouwd in microsoft access, die werd gecreëerd om de registratie en analyse van lithische artefacten op verschillende niveaus, en met verschillende vraagstellingen in het achterhoofd, mogelijk te maken. Hierbij staat flexibiliteit en gebruiksgemak centraal. De databank is vrij beschikbaar voor iedereen die ze wil gebruiken.

In this study, an investigation over digestive diseases has been done in which the sound acts as a detector medium. Pursue to the preprocessing the extracted signal in cepstrum domain is registered. After classification of digestive diseases, the system selects random samples based on their features and generates the interest nonstationary, long-term signals via inverse transform in cepstral domain which is presented in digital and sonic form as the output. This structure is updatable or on the other word, by receiving a new signal the corresponding disease classification is updated in the feature domain., {"references": ["", "M. Akay, 4 Processing, Academic signal. Biomedical press 1994.", "Rangayyan R.M, Biomedical signal Analysis, IEEE Press _ wiley, 2002.", "Cohn A., Biomedical signal Processing, Vol. 1& 2, CR Press 1986.", "Mendel J.M Lessons in Estimation theory for control, Prentice Hall, 1995 MIT.BIH.", "Roffe Y., Einav S., Liaw J., and Keren G., ''Cepstrum Analysis of Reflected Pressure Waves in stenosed Arteries\", Department of Biological Engineering & Computing, 34, pp 175_180, 1996.", "Lizzi, F.L., Feleppa, E.J., Yaremko, M .M., King, D .L.and wai ,P, \"Liver_tissue characterization by digital spectrum and cepstrum analysis\"Proceeding of the 1980 IEEE Ultrasound Symposium. McAvoy B. R. New York, Institute of Electrical and Electronics Engineers, 1981, pp 575_578.", "Mitev, P. ,Hadjitodorov, S."Cepstrum method for fundamental frequency evaluation pathological voices."In: Proc.Of VIII National Conf. Biomedical Physics and Engineering, Sofia, Oct.2000,pp.54_57."]}

Purpose: To assess the expression of pRb and EGFR in oral epithelial lesions using TMA technique. Methods: Paraffin embedded specimens were selected from the archives of the Diagnostic Laboratories at the Faculty of Dentistry, University of Malaya and Universiti Kebangsaan Malaysia. Selected epithelial areas were cored and developed into tissue microarray blocks. A total of 32 samples for pRb and 50 samples for EGFR were analysed using immunohistochemical techniques. Results: Abnormal pRb staining (1+ or less) was seen in 80% (8/10) OSCC, 38.5% (5/13) dysplastic and 22.2% (2/9) normal/hyperplastic epithelium. Positive EGFR staining was observed in 25/35 (71.4%) OSCC cases with 11 cases (31.4%) showing staining of 2+ or more. Out of 5 epithelial dysplasia and 10 normal/hyperplastic epithelium, positive EGFR staining was noted in 3 (60%) and 8 (80%) cases respectively. Interestingly, only 1(20%) epithelial dysplasia and 1(10%) normal/hyperplastic epithelium showed staining of 2+ or more. Conclusion: High frequency of abnormal expression of pRb is observed in OSCC cases as compared to dysplastic and normal/hyperplastic epithelium. EGFR is expressed in OSCC as well as epithelial dysplasia and normal/hyperplastic epithelium. However, higher EGFR positivity is more common in OSCC cases. This study supports the feasibility and ease of using TMA technique for immunohistochemical studies. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Purpose: Human b-Defensin (hBDs) is an epithelial origin, cationic antimicrobial peptides. Their expression is reported in oral mucosa, gingiva and salivary glands. However, their protective role is not well understood. It has been suggested that bacterial and viral infection are an important factors in the process of carcinogenesis. This study investigated the expression of hBD-3 in oral squamous cells carcinoma (OSCC). Methods: 29 OSCC and 23 samples of normal and dysplastic tissues were obtained from Oral Pathology diagnostic laboratory, faculty of Dentistry, UKM and UM. The tissues were arrayed and the expression of hBD-3 was analysed using immunohistochemistry technique. Results: Higher levels of hBD-3 expression were found in stage II and II of OSCC compared to stage I and IV. Expression of hBD-3 was also seen higher in the tumour front arrays. Healthy and dysplastic tissues also seen to expressed hBD-3 but most are moderately stained. Conclusion: High levels of hBD-3 expression is seen especially in stage II and III as well as in tumour front suggest a potential tumour marker, correlation between level of expression and probable infective cancer causes need to be investigate further. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Introduction: The variability of clinical outcomes in oral cancer patients and the heterogeneity of the disease are the main challenges for the improvement of current treatment modalities. Efforts in our laboratory have focused on the molecular profiling of oral cancer to understand the mechanisms underlying the disease. In a previous microarray study, we found Guanine nucleotide binding protein alpha-12 (GNA12) to be up-regulated in oral cancer. Materials and methods: In this study, we validated the expression of GNA12 at the mRNA level in 47 oral squamous cell carcinoma (OSCC) and 18 non-malignant oral mucosa tissues, by quantitative polymerase chain reaction (qPCR). Further, GNA12 protein expression was accessed by immunohistochemistry (IHC) on 44 tumors and 23 non-malignant oral mucosa tissues. Using OSCC cell lines, we examined the effects of GNA12 signaling by in vitro functional assays. Results: We demonstrated that GNA12 mRNA levels were significantly up-regulated in OSCC in comparison to the non-malignant oral mucosa tissues. Consistently, high levels of GNA12 protein expression were detected in 75% of OSCC tissues, while the non-malignant tissues showed negative or weak expression. We demonstrated that expression of activated GNA12 (GQ231L) promoted oral cancer cell migration in a monolayer wound healing assay and cell invasion through the matrigel barrier, but cell proliferation was not changed. Correspondingly, exogenous expression of the regulator of G-protein signaling (RGS) blocked G12 family signaling through RhoA, which resulted in the inhibition of cancer cell migration and invasion. Discussion: In summary, we demonstrated for the first time in OSCC that GNA12 is over-expressed in a large percentage of these patients and notably, the over-expression drives migration and invasion of oral cancer cells. Taken together, this information indicates that targeting GNA12 could benefit oral cancer patients by preventing the spread of the disease. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Darier disease, also known as keratosis follicularis, dyskeratosis follicularis, and benign dyskeratosis, is a rare disorder of keratinisation that primarily affects the skin and, to a lesser extent, the oral mucosa. It was described independently by both Darier and White in 1889. It has a prevalence of 1:100,000 of the population and is inherited as an autosomal dominant trait.1 The age of onset is childhood or adolescence. Patients usually present with multiple small firm reddish-brown papules on the forehead, scalp, neck, shoulders, chest, and limbs. Other cutaneous signs include punctate keratotic pits of the palms and soles and dystrophy of the nails, which is characterised by a red and white sandwich of streaks associated with a V-shaped notch. The oral lesions are usually asymptomatic and are seen as multiple, normal-coloured or white flat-topped papules that predominantly affect the palate. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

International audience; Depuis les premières conférences internationales sur l'environnement et la gestion durable des forêts, la demande européenne d'identifier le plus exactement possible l'originalité des habitats encore en place, de contrôler leur conservation, voire leur restauration, s'est faite de plus en plus pressante. Dans les pays d'Europe occidentale, on connaît relativement bien les principales exigences écologiques des espèces grâce à des travaux de compilation importants dont le plus clair et le mieux illustré est celui de Rameau et al. (1989). Plus tard, Bartoli et al. (2000) mettent au point le logiciel "ECOFLORE". L'originalité du présent travail par rapport à celui de nos prédécesseurs réside dans l'adoption d'une banque informatisée de données sous format numérique et adaptée au territoire couvert par la Nouvelle flore de la Belgique et des Régions voisines (Lambinon et al., 2004). L'utilisation combinée de deux logiciels du commerce permet de visualiser rapidement les gammes communes des exigences hydriques et trophiques de toutes les espèces d'un relevé ou d'un groupe de relevés. Le repérage et le classement des groupes socio-écologiques assisté par ordinateur permettent de constituer des assemblages bien précis dont il faut apprécier la cohérence et la fonctionnalité.

Introduction: We have established a cell line ORL-48(T) from a surgically resected specimen of an untreated primary human oral squamous cell carcinoma of the mandible.This patient did not appear to have any oral habits which have been reported to be associated with oral cancer Materials and Method: The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, HPV infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of the ORL-48(T) cell line were investigated. Results: The ORL-48(T) cell line is immortal, 3T3- independent and grew as a monolayer with the doubling time of 48h. Immunohistochemistry staining of cytokeratins confirmed the cell line is of epithelial origin. Soft agar assays demonstrated that ORL-48(T) expressed a low degree of anchorage independency (CFE

The authors present a study conducted in the northeast of the Province of Venice, in the area falling between the Sile and Livenza rivers, where depressions with elevations below the average sea level alternate with hillocks of fluvial origin. The evolution of this territory has been influenced by numerous environmental factors such as periodic flooding, changes in sea level and the progressive phenomenon of subsidence. Over time, the complexity of the situation has been further compounded by the action of the rivers that presently flow through the territory itself: via aerial photogrametry and remote sensing it is possible to trace their former courses, associated with repeated processes of erosion and sedimentation that have conspicuously modelled the surface of this plain area lying in proximity to the coastline. Added to these factors is the impact of numerous human interventions aimed at diverting the main watercourses (Piave, Livenza) and reclaiming wetland areas. The study involved a sequence of stages. First of all the territory was divided into homogenous Landscape Units, coded according to the criteria defined in the catalogue of landscapes of the Venetian plain; said units were identified on the basis of GIS ArcView information technology, using the Geomorphological Map of the provincial - mainland territory (DOGE project) and the microrelief map and digital models derived therefrom as the basic mapping resources. The soil and geological maps already available were used for purposes of thematic verification and calibration. Pedological data derived from georeferenced field surveys were processed and soils were classified according to profile type and area distribution of several physical and chemical properties. This made it possible to define associations and relations among the soils present, such as to provide a basis for computer-aided soil mapping.

Aim: A pilot study was carried out in the state of Kelantan, north-east of Malaysia, to determine the feasibility of using pathological and hospital databases to acquire incidence on oral cancer. Method: Data on OC patients (ICD-10: C00.3-00.5, C02-06) were obtained from incident surgical biopsy records of the two main referral centres for head and neck surgery in Kelantan namely, the Department of Pathology, Faculty of Medicine, University of Science and the Oral Surgery clinic, Kota Baru Hospital between January 1994 to December 1998. The crude and age-specific incidence rates were calculated based on the annual population census of Kelantan and this was standardised to the world population. Results: There were forty-three OC cases during that period. The incidence of OC was 6.1 per million annually (males, 1.4 per million; females, 1.0 per million). The age-standardised incidence was 1.7 per million. The main leading sites of OC were the gum and the buccal mucosa/vestibule/retromolar areas. Conclusion: Pathological and hospital records provide an alternative way of obtaining important epidemiological data on OC, crucially in places where population-based cancer registries have not been established. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

Apresenta modelo de biblioteca jurídica que sugere a potencialização do sistema de informações, sem a necessidade de aumento físico considerável do acervo interno. Sua estrutura, apoiada na conexão com bancos e bases de dados, redes eletrônicas de comunicação e bibliotecas informatizadas situadas interna e externamente, permitirá acesso a um conjunto de fontes e acervos muito mais amplo e diversificado do que aquele que existe internamente na forma tradicional vindo completar esta estrutura. It presents model of law library that suggests the potentiality of the information's system, without the necessity of considerable physical increase of the internal quantity. Its structure, supported by connection with banks and databases, electronic networks of communication and situated eletronics libraries intern and external, will allow to access to a much more ample and diversified set of sources and quantities than the one that, in traditional form, exists internally completing this structure.

The expression of Tenascin-C, an adhesion modulating or anti- adhesive extracellular matrix protein, was evaluated in betel quid associated lesions of oral mucosa showing focal hyperkeratosis (n=15), dysplasia (n=15), oral submucous fibrosis (n=7) and oral squamous cell carcinoma (n=8). All the patients were chewers of betel quid containing betel leaves, areca nuts, lime and tobacco. The results obtained from this study were compared to similar lesions in smokers and non-quid users cited in the literature (Shrestha et al, Oral Oncol, 1994). An enhanced expression of TN-C was observed in epithelial connective tissue interface of quid associated hyperkeratotic and dyplastic mucosa. However, the expression was less extensive as compared to similar lesions among smokers or those without smoking or quid habits although the enhanced expression observed in the stromal tissue surrounding the carcinoma cells in carcinoma arising at sites where quids were often placed did not differ. Oral submucous fibrosis showed no or a faint linear immunoreactivity to an intense reactivity often abruptly ending at the epithelial-connective tissue interface. Areas of subepithelial hyalinization in oral submucous fibrosis remained unreactive. These variations in oral submucous fibrosis were observed in different areas of the same specimen. Oral squamous cell carcinoma, however, showed no difference in the expression of TN-C among these two groups of patients. The result of the present study suggest that epithelial hyperproliferation with or without dysplastic changes in quid related oral lesions may induce an altered epithelial-mesenchymal interactions and may be a factor in an enhanced expression of TH-C. However, the reaction products may be less widespread as compared to lesions among smokers or those without smoking or quid habits. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

A retrospective study of odontogenic keratocysts in the Malaysia-Singapore region was done. The purpose of this study is to present the clinicopathological features of odontogenic keratocysts in the Oriental population and to compare the data with those from other reported studies. Biopsy records from 1981 to 1992 of 61 cases of odontogenic keratocysts in Malaysia and Singapore showed that there were 42.6% female and 57.4% male patients. The cysts occurred in 75.4% Chinese, 6.6% Malays, 9.8% Indian, and 8.2% other ethnic groups. The mean age of these patients was 26.98 ± 15.38 years with peak incidence in the second to fourth decades. The location of the lesion was more often in the mandible (65.5%) than the maxilla (31.0%). There is marked predilection for the posterior mandible. Odontogenic keratocysts presenting at the site of dentigerous a cyst was observed in 7 cases (11.5%). Histologically, 90.2% of the cyst were lined by parakeratinised stratified squamous epithelium while only 3.3% of the cyst with orthokeratinised stratified squamous epithelium. Mixed parakeratinised and orthokeratinised epithelial lining was observed in 4 cases (6.5%). Inflammation of cyst wall was found in 42 cases (68.8%). Stellate-like inflamed lining epithelium was observed in 9.8% of the cases while 6.6% of the cases demonstrated Rushton bodies in the epithelial lining. Keratin in the lumen was observed in 19.7% of the cases. Satellite cyst was observed in only 6 cases (9.8%). In conclusion, most clinical and histological features seen in this study similar to that for the Caucasian. The only clinical feature that differs is the peak incidence which ranged from the second to fourth decades with an absence of the second peak for our study. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.

The aim of this study was to determine the periodontal status and treatment needs among the Indian estate population. The Community Periodontal Index of Treatment Needs was used to obtained clinical data on 208 Indian subjects residing in three estates in the state of Selangor. The prevalence of periodontal disease among the subjects was 100. Less than 1 of the subjects had bleeding on probing as the highest CPITN score. About 97 of the subjects showed evidence of periodontal pocket of 4mm or more. Of these, half showed pocketing of 6mm or more. No statistically significant difference (p> 0.05) was observed between males and females in terms of the most severe periodontal conditions encountered. About 2.5, 1.6 and 0.9 sextants were involved with pocketing of 4-5mm, calculus and pocketing of 6mm or more respectively. Again this pattern of sextants involvement shows no significant difference by gender (p>0.05). All subjects required oral hygiene instruction and 97.4 required scaling. Complex periodontal care was required by 48.7 of the subjects. The findings of this study indicate an urgent need for an oral health promotion programme to be instituted aimed at the prevention of periodontal disease and the utilization of appropriate manpower for the control and management of the existing disease in the Indian estate population. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.