824 results on '"Zhuo A. Chen"'
Search Results
102. Supplementary Figures 1 - 6 from RRM2 Regulates Bcl-2 in Head and Neck and Lung Cancers: A Potential Target for Cancer Therapy
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Dong M. Shin, Zhuo (Georgia) Chen, Xingming Deng, Gabriel Sica, Zhibo Wang, Zhengjia Chen, Sreenivas Nannapaneni, Lydia Koenig, Dongsheng Wang, A.R.M. Ruhul Amin, and Mohammad Aminur Rahman
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PDF file - 427K, Figure S1. (A) Western blotting was performed for the indicated proteins in Tu212 and Bcl-2 overexpressing Tu212 (Tu212/Bcl-2) cell lines (upper panel) and in Tu686 and Bcl-2 overexpressing Tu686 (Tu686/Bcl-2) cell lines (lower panel) (B) Apoptosis analysis (error bars are mean � SD from 3 independent experiments) of indicated cell lines upon treatment with siC or siR2. Figure S2. (A) Western blotting was performed for the indicated proteins in H1299 and two clones of dominant negative p73 overexpressing H1299 (H1299/dN p73 cl-7 and cl-10) cell lines (upper panel) and in H358 dominant negative p73 overexpressing H358 (H358/dN) cell lines (lower panel). (B) Apoptosis analysis (error bars are mean � SD from 3 independent experiments) of indicated cell lines upon treatment with siC or siR2. Figure S3. (A) Western blotting was performed for the indicated proteins in A549 and Akt overexpressing A549 (A549/Akt) cell lines (upper panel) and in Tu686 and Akt overexpressing Tu686 (Tu686/Akt) cell lines (lower panel) (B) Apoptosis analysis (error bars are mean � SD from 3 independent experiments) of indicated cell lines upon treatment with siC or siR2. Figure S4. Gallery images of a z-stack. Tu212 cells were stained with anti-RRM2 (green), anti-Bcl-2 (red) and DAPI (blue). A z-stack of optical sections was created at 0.59 μm intervals using a confocal microscope (LSM 510; Carl Zeiss MicroImaging, Inc ) collected Inc.). A total of 32 images were collected, shown in a gallery of merged images collected in the z-stack in a single display. Figure S5. The relationship between RRM2 (IHC) and RRM2 (QD-IHF) in HNSCC. Spearman's correlation coefficient was estimated between RRM2 (IHC) and RRM2 (QD-IHF) expression level in tumor tissues from HNSCC patients. Figure S6. The relationship between RRM2 (IHC) and RRM2 (QD-IHF) in NSCLC. Spearman's correlation coefficient was estimated between RRM2 (IHC) and RRM2 (QD-IHF) expression levels in tumor tissues from NSCLC patients.
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- 2023
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103. Supplementary Figure 3 from HER3 Targeting Sensitizes HNSCC to Cetuximab by Reducing HER3 Activity and HER2/HER3 Dimerization: Evidence from Cell Line and Patient-Derived Xenograft Models
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Zhuo G. Chen, Nabil F. Saba, Dong M. Shin, Fadlo R. Khuri, Zhengjia Chen, J. Trad Wadsworth, Mark El-Deiry, Mihir Patel, Michael Rossi, A.R.M. Ruhul Amin, Sreenivas Nannapaneni, Kelly R. Magliocca, Hongzheng Zhang, Guoqing Qian, and Dongsheng Wang
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PDX tumor growth of patient 2,4,5 under the treatment of indicated drug
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- 2023
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104. Supplementary Figure 4 from The Pivotal Role of Integrin β1 in Metastasis of Head and Neck Squamous Cell Carcinoma
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Zhuo G. Chen, Dong M. Shin, Mourad Tighiouart, Zhengjia Chen, Lydia Koenig, Sreenivas Nannapaneni, Mohammad Aminur Rahman, Zhongliang Hu, Ling Su, Donghai Huang, A.R.M. Ruhul Amin, Susan Müller, and Dongsheng Wang
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PDF file, 58K, Over expression of MMP-2 in M4E-15 integrin beta 1 knock-down cells increased the invasive capability.
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- 2023
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105. Supplementary Figure 2 from The Pivotal Role of Integrin β1 in Metastasis of Head and Neck Squamous Cell Carcinoma
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Zhuo G. Chen, Dong M. Shin, Mourad Tighiouart, Zhengjia Chen, Lydia Koenig, Sreenivas Nannapaneni, Mohammad Aminur Rahman, Zhongliang Hu, Ling Su, Donghai Huang, A.R.M. Ruhul Amin, Susan Müller, and Dongsheng Wang
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PDF file, 359K, OS and DFS analysis of chemotherapy on all patients.
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- 2023
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106. Data from Honokiol Radiosensitizes Squamous Cell Carcinoma of the Head and Neck by Downregulation of Survivin
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Dong M. Shin, Jack L. Arbiser, Zhuo Georgia Chen, Nabil F. Saba, Xingming Deng, Zhengjia Chen, Sungjin Kim, Sreenivas Nannapaneni, Jun Zhang, Amy Y. Chen, Mihir R. Patel, Kelly Magliocca, Guoqing Qian, Guo Chen, Wen Huang, Jonathan J. Beitler, and Xu Wang
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Purpose: Previous studies revealed diverging results regarding the role of survivin in squamous cell carcinoma of the head and neck (SCCHN). This study aimed to evaluate the clinical significance of survivin expression in SCCHN; the function of survivin in DNA-damage repair following ionizing radiation therapy (RT) in SCCHN cells; and the potential of honokiol to enhance RT through downregulation of survivin.Experimental Design: Expression of survivin in SCCHN patient primary tumor tissues (n = 100) was analyzed and correlated with clinical parameters. SCCHN cell lines were used to evaluate the function of survivin and the effects of honokiol on survivin expression in vitro and in vivo.Results: Overexpression of survivin was significantly associated with lymph nodes' metastatic status (P = 0.025), worse overall survival (OS), and disease-free survival (DFS) in patients receiving RT (n = 65, OS: P = 0.024, DFS: P = 0.006) and in all patients with SCCHN (n = 100, OS: P = 0.002, DFS: P = 0.003). In SCCHN cells, depletion of survivin led to increased DNA damage and cell death following RT, whereas overexpression of survivin increased clonogenic survival. RT induced nuclear accumulation of survivin and its molecular interaction with γ-H2AX and DNA-PKCs. Survivin specifically bound to DNA DSB sites induced by I-SceI endonuclease. Honokiol (which downregulates survivin expression) in combination with RT significantly augmented cytotoxicity in SCCHN cells with acquired radioresistance and inhibited growth in SCCHN xenograft tumors.Conclusions: Survivin is a negative prognostic factor and is involved in DNA-damage repair induced by RT. Targeting survivin using honokiol in combination with RT may provide novel therapeutic opportunities. Clin Cancer Res; 24(4); 858–69. ©2017 AACR.
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- 2023
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107. Sppelementary Figures from Phase Ib Study of Chemoprevention with Green Tea Polyphenon E and Erlotinib in Patients with Advanced Premalignant Lesions (APL) of the Head and Neck
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Nabil F. Saba, Zhuo G. Chen, Fadlo R. Khuri, Suresh S. Ramalingam, Taofeek K. Owonikoko, Adam M. Klein, Steven M. Roser, Conor E. Steuer, Jonathan J. Beitler, Mark W. El-Deiry, Amy Y. Chen, Zhengjia Chen, Yuan Liu, Qiuying Shi, Mihir R. Patel, Sreenivas Nannapaneni, and Dong M. Shin
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Supplementary Figures S1A & S1B.
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- 2023
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108. Data from Chemoprevention of Head and Neck Cancer by Simultaneous Blocking of Epidermal Growth Factor Receptor and Cyclooxygenase-2 Signaling Pathways: Preclinical and Clinical Studies
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Zhuo Georgia Chen, Fadlo R. Khuri, Jennifer R. Grandis, Hyung Ju C. Shin, Zhengjia Chen, Sufi M. Thomas, Jonathan J. Beitler, Rachel Moreno-Williams, William J. Grist, Johann C. Brandes, Scott Kono, Gabriel Sica, Melinda Lewis, Susan Müller, A.R.M. Ruhul Amin, Sreenivas Nannapaneni, Amy Y. Chen, Nabil F. Saba, Hongzheng Zhang, and Dong M. Shin
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Purpose: We investigated the efficacy and underlying molecular mechanism of a novel chemopreventive strategy combining EGF receptor (EGFR) tyrosine kinase inhibitor (TKI) with cyclooxygenase-2 inhibitor (COX-2I).Experimental Design: We examined the inhibition of tumor cell growth by combined EGFR-TKI (erlotinib) and COX-2I (celecoxib) treatment using head and neck cancer cell lines and a preventive xenograft model. We studied the antiangiogenic activity of these agents and examined the affected signaling pathways by immunoblotting analysis in tumor cell lysates and immunohistochemistry (IHC) and enzyme immunoassay (EIA) analyses on the mouse xenograft tissues and blood, respectively. Biomarkers in these signaling pathways were studied by IHC, EIA, and an antibody array analysis in samples collected from participants in a phase I chemoprevention trial of erlotinib and celecoxib.Results: The combined treatment inhibited head and neck cancer cell growth significantly more potently than either single agent alone in cell line and xenograft models, and resulted in greater inhibition of cell-cycle progression at G1 phase than either single drug. The combined treatment modulated the EGFR and mTOR signaling pathways. A phase I chemoprevention trial of combined erlotinib and celecoxib revealed an overall pathologic response rate of 71% at time of data analysis. Analysis of tissue samples from participants consistently showed downregulation of EGFR, pERK, and pS6 levels after treatment, which correlated with clinical response.Conclusion: Treatment with erlotinib combined with celecoxib offers an effective chemopreventive approach through inhibition of EGFR and mTOR pathways, which may serve as potential biomarkers to monitor the intervention of this combination in the clinic. Clin Cancer Res; 19(5); 1244–56. ©2013 AACR.
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- 2023
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109. Data from CXC Chemokine Receptor-4 Antagonist Blocks Both Growth of Primary Tumor and Metastasis of Head and Neck Cancer in Xenograft Mouse Models
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Hyunsuk Shim, Zhuo (Georgia) Chen, Mark M. Goodman, Dong M. Shin, Heidi T. Cho, Aizhi Zhu, Mison Choe, Xin Zhang, Zhongxing Liang, and Younghyoun Yoon
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Squamous cell carcinoma of the head and neck (SCCHN) metastasizes to the lymph nodes and lungs. We have generated previously an orthotopic mouse model for head and neck metastasis and did in vivo selection of SCCHN cells through four rounds of serial metastases. A subpopulation of 686LN cells with high metastatic potential (686LN-Ms) was isolated. When the highly metastatic cells were compared with their low metastatic parental cells (686LN-Ps), we found that CXC chemokine receptor-4 (CXCR4) mRNA levels were significantly higher in the 686LN-Ms cells than the 686LN-Ps cells. Interestingly, the metastatic subclones had lost epithelial morphology and acquired mesenchymal features, which were maintained during cell expansion in vitro. This was featured by decreased E-cadherin and involucrin and increased vimentin and integrin β1. These results imply that CXCR4 and epithelial-mesenchymal transition markers can be potential biomarkers to identify the subpopulation of cells with high metastatic potential. Using the orthotopic SCCHN animal model, we showed that anti-CXCR4 treatment suppressed primary tumor growth by inhibiting tumor angiogenesis and prevented lung metastasis. Because the reduction of metastasis seen in the treated group could have resulted from 2-fold reduction in primary tumor size compared with that in the control group, we examined the effects of the CXCR4 antagonist in an experimental metastatic animal model in which 686LN-Ms cells were i.v. injected. 686LN-Ms cells failed to metastasize in the CXCR4 antagonist-treated group, whereas they metastasized to the lungs in the control group. Our data indicate that CXCR4 is an important target to inhibit tumor progression in SCCHN. [Cancer Res 2007;67(15):7518–23]
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- 2023
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110. Supplementary Methods, Figures 1-2 from Detection of Circulating Tumor Cells in Human Peripheral Blood Using Surface-Enhanced Raman Scattering Nanoparticles
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Dong M. Shin, Shuming Nie, Hyung Ju C. Shin, Melinda M. Lewis, Fadlo R. Khuri, Zhuo Georgia Chen, Jonathan J. Beitler, Ximei Qian, and Xu Wang
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Supplementary Methods, Figures 1-2 from Detection of Circulating Tumor Cells in Human Peripheral Blood Using Surface-Enhanced Raman Scattering Nanoparticles
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- 2023
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111. Supplementary Figures 1-4 from CXC Chemokine Receptor-4 Antagonist Blocks Both Growth of Primary Tumor and Metastasis of Head and Neck Cancer in Xenograft Mouse Models
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Hyunsuk Shim, Zhuo (Georgia) Chen, Mark M. Goodman, Dong M. Shin, Heidi T. Cho, Aizhi Zhu, Mison Choe, Xin Zhang, Zhongxing Liang, and Younghyoun Yoon
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Supplementary Figures 1-4 from CXC Chemokine Receptor-4 Antagonist Blocks Both Growth of Primary Tumor and Metastasis of Head and Neck Cancer in Xenograft Mouse Models
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- 2023
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112. Data from Detection of Circulating Tumor Cells in Human Peripheral Blood Using Surface-Enhanced Raman Scattering Nanoparticles
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Dong M. Shin, Shuming Nie, Hyung Ju C. Shin, Melinda M. Lewis, Fadlo R. Khuri, Zhuo Georgia Chen, Jonathan J. Beitler, Ximei Qian, and Xu Wang
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The detection and characterization of circulating tumor cells (CTC) holds great promise for personalizing medicine and optimizing systemic therapy. However, low specificity, low sensitivity, and the time consuming nature of current approaches have impeded clinical adoption. Here we report a new method using surface-enhanced Raman spectroscopy (SERS) to directly measure targeted CTCs in the presence of white blood cells. SERS nanoparticles with epidermal growth factor peptide as a targeting ligand have successfully identified CTCs in the peripheral blood of 19 patients with squamous cell carcinoma of the head and neck (SCCHN), with a range of 1 to 720 CTCs per milliliter of whole blood. Our technique may provide an important new clinical tool for management of patients with SCCHN and other cancers. Cancer Res; 71(5); 1526–32. ©2011 AACR.
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- 2023
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113. Integrative structural analysis of the type III secretion system needle complex from Shigella flexneri
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Lara Flacht, Michele Lunelli, Karol Kaszuba, Zhuo Angel Chen, Francis J. O'. Reilly, Juri Rappsilber, Jan Kosinski, and Michael Kolbe
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secretion ,T3SS ,bacteria ,Molecular Biology ,Biochemistry ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit ,infection ,Shigella flexneri ,type III secretion system - Abstract
The type III secretion system (T3SS) is a large, transmembrane protein machinery used by various pathogenic gram‐negative bacteria to transport virulence factors into the host cell during infection. Understanding the structure of T3SSs is crucial for future developments of therapeutics that could target this system. However, much of the knowledge about the structure of T3SS is available only for Salmonella, and it is unclear how this large assembly is conserved across species. Here, we combined cryo‐electron microscopy, cross‐linking mass spectrometry, and integrative modeling to determine the structure of the T3SS needle complex from Shigella flexneri. We show that the Shigella T3SS exhibits unique features distinguishing it from other structurally characterized T3SSs. The secretin pore complex adopts a new fold of its C‐terminal S domain and the pilotin MxiM[SctG] locates around the outer surface of the pore. The export apparatus structure exhibits a conserved pseudohelical arrangement but includes the N‐terminal domain of the SpaS[SctU] subunit, which was not present in any of the previously published virulence‐related T3SS structures. Similar to other T3SSs, however, the apparatus is anchored within the needle complex by a network of flexible linkers that either adjust conformation to connect to equivalent patches on the secretin oligomer or bind distinct surface patches at the same height of the export apparatus. The conserved and unique features delineated by our analysis highlight the necessity to analyze T3SS in a species‐specific manner, in order to fully understand the underlying molecular mechanisms of these systems. The structure of the type III secretion system from Shigella flexneri delineates conserved and unique features, which could be used for the development of broad‐range therapeutics.
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- 2023
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114. International REACH Forgiveness Intervention: A Multi-Site Randomized Controlled Trial
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Man Yee Ho, Everett Worthington, Richard Cowden, Andrea Ortega Bechara, Zhuo Job Chen, Elly Yuliandari Gunatirin, Shaun Joynt, Viacheslav V. Khalanskyi, Hennadii Korzhov, Ni Made Taganing Kurniati, Nicole Rodriguez, Anastasiya Salnykova, Liudmyla Shtanko, Sergiy Tymchenko, Vitaliy L. Voytenko, Anita Zulkaida, Maya B Mathur, and Tyler VanderWeele
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Importance: Forgiveness interventions have been shown in prior randomized trials to reduce depression and anxiety symptoms. Most such interventions require trained therapists, limiting scalability. Objective: To determine whether a brief self-directed forgiveness workbook intervention could alter forgiveness, depression symptoms, and anxiety symptoms. Design: A multi-site randomized waitlist-controlled trial was conducted among 4,598 participants. Recruitment occurred from February 11, 2020 to September 30, 2021. Final follow-up occured October 25, 2021. Setting: Participants were recruited from community-based samples in sites in Colombia, Hong Kong, Indonesia, South Africa, and Ukraine. Participants: Individuals (N = 7,837) were screened for eligibility. For inclusion, participants needed to be ≥18 years and have experienced an interpersonal transgression. Based on these criteria, 4,786 individuals were randomized, but 55 individuals were excluded for suspicious/fraudulent online participation, and 133 were excluded for not providing age or indicating age below 18. The analytic sample consisted of N = 4,598. Interventions: At each site, participants were randomly assigned to either immediate receipt of a self-directed forgiveness workbook intervention, or to receipt after a two-week delay. Main Outcomes and Measures: The primary outcomes were unforgiveness (Transgression-Related Interpersonal Motivations Inventory-18), depression symptoms, and anxiety symptoms (Brief Symptom Inventory-18) measured at two weeks following intervention assignment. Results: The study sample was median age 26 and 73% female. At two weeks follow-up, unforgiveness was lower among the immediate-treatment group compared to the delayed-treatment group (standardized mean difference=-0.52 [95% CI=-0.58, -0.46]); similar patterns were found for depression (standardized mean difference=-0.22 [95% CI=-0.28, -0.16]) and anxiety symptoms (standardized mean difference=-0.21 [95% CI=-0.27, -0.15]). Conclusions and Relevance: A brief workbook intervention promoted forgiveness and reduced depression and anxiety symptoms. The promotion of forgiveness with such workbooks has the potential for widespread dissemination to improve global mental health. Trial Registration: ClinicalTrials.gov Identifier: NCT04257773.
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- 2023
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115. A Minimum Spanning Forest-Based Method for Noninvasive Cancer Detection With Hyperspectral Imaging.
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Robert Pike 0003, Guolan Lu, Dongsheng Wang 0001, Zhuo Georgia Chen, and Baowei Fei
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- 2016
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116. An Improved Joint Sparse Representation of Array Covariance Matrices Approach in Multi-source Direct Position.
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Zhuo-Hao Chen, Kai Yu 0005, Ji-an Luo, and Zhi Wang 0003
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- 2014
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117. Application and Comparison of Discovery Model and Others in Petroleum Resource Assessment
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Qiu-Lin, Guo, Wei, Yan, Zhuo-Heng, Chen, Blondel, Philippe, Series editor, Reitner, Joachim, Series editor, Stüwe, Kurt, Series editor, Trauth, Martin H., Series editor, Yuen, David A., Series editor, Pardo-Igúzquiza, Eulogio, editor, Guardiola-Albert, Carolina, editor, Heredia, Javier, editor, Moreno-Merino, Luis, editor, Durán, Juan José, editor, and Vargas-Guzmán, Jose Antonio, editor
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- 2014
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118. Retrofit Fatigue Cracked Diaphragm Cutouts Using Improved Geometry in Orthotropic Steel Decks
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Zhuo-Yi Chen, Chuan-Xi Li, Jun He, and Hao-Hui Xin
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orthotropic steel deck ,fatigue rehabilitation ,diaphragm cutout ,stress spectrum ,fatigue assessment ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Diaphragm cutouts are set to release redundant constraints and hence reduce weld fatigue at the connection of U-ribs to diaphragms in orthotropic steel decks. However, most fatigue cracks which originate from the edge of cutouts are in fact detected in the diaphragms. Therefore, a retrofit technology on cracked cutouts at the diaphragm is proposed and applied to the orthotropic steel box girder of a suspension bridge. Firstly, the stress concentration on the cutout is analyzed through refined finite element analyses. Furthermore, the fatigue cracked cutouts are retrofitted by changing their geometrical parameters. Thereafter, an optimized geometry and the size of diaphragm cutouts were confirmed and applied in the rehabilitation of a suspension bridge. On-site wheel load tests were carried out before and after retrofitting of the diaphragm cutout. The stress distributions along the edges of the cutouts and at the side of a diaphragm were measured under a moving vehicle. The stress spectra at two critical locations on the edge of a cutout was obtained under longitudinally and laterally moving vehicles. Finally, the fatigue life of the cutouts is assessed by the modified nominal stress method. The analytical and test results indicate that the wheel loads on the deck transmit stress to the diaphragms through the U-ribs, during the load transmission process, the stress flow is obstructed by diaphragm cutouts, resulting in local stress concentrations around the cutouts. In addition, the overall size of the cutouts should be small, but the radius of the transition arc should be large, thus the stress flow will not be obviously obstructed. After the retrofitting of the cutouts by improved geometry, the maximum stress decreases by 87.6 MPa, which is about 40% of the original stress. The equivalent constant amplitude stress is reduced by 55.2% when the lateral position of the wheel loads is taken into consideration. Based on the stresses obtained by finite element analysis (FEA) and experimental tests, the fatigue lives of the original cutouts are 1.7 and 4.9 years, respectively, which increase to 78.1 and 155.5 years, respectively, after the cutouts were retrofitted, which indicates that the improved geometry and retrofit technology can enhance the fatigue performance and extend the fatigue life of diaphragm cutouts with fatigue cracks.
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- 2020
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119. Tumor Membrane Vesicle Vaccine Augments the Efficacy of Anti-PD1 Antibody in Immune Checkpoint Inhibitor-Resistant Squamous Cell Carcinoma Models of Head and Neck Cancer
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Ramireddy Bommireddy, Luis E. Munoz, Anita Kumari, Lei Huang, Yijian Fan, Lenore Monterroza, Christopher D. Pack, Sampath Ramachandiran, Shaker J.C. Reddy, Janet Kim, Zhuo G. Chen, Nabil F. Saba, Dong M. Shin, and Periasamy Selvaraj
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cancer ,vaccine ,adjuvants ,IL-12 ,TMVs ,MOC1 ,Medicine - Abstract
Immune checkpoint inhibitor (ICI) immunotherapy improved the survival of head and neck squamous cell carcinoma (HNSCC) patients. However, more than 80% of the patients are still resistant to this therapy. To test whether the efficacy of ICI therapy can be improved by vaccine-induced immunity, we investigated the efficacy of a tumor membrane-based vaccine immunotherapy in murine models of HNSCC. The tumors, grown subcutaneously, are used to prepare tumor membrane vesicles (TMVs). TMVs are then incorporated with glycolipid-anchored immunostimulatory molecules GPI-B7-1 and GPI-IL-12 by protein transfer to generate the TMV vaccine. This TMV vaccine inhibited tumor growth and improved the survival of mice challenged with SCCVII tumor cells. The tumor-free mice survived for several months, remained tumor-free, and were protected following a secondary tumor cell challenge, suggesting that the TMV vaccine induced an anti-tumor immune memory response. However, no synergy with anti-PD1 mAb was observed in this model. In contrast, the TMV vaccine was effective in inhibiting MOC1 and MOC2 murine oral cancer models and synergized with anti-PD1 mAb in extending the survival of tumor-bearing mice. These observations suggest that tumor tissue based TMV vaccines can be harnessed to develop an effective personalized immunotherapy for HNSCC that can enhance the efficacy of immune checkpoint inhibitors.
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- 2020
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120. Artificial Bee Colony Algorithm for Modular Neural Network.
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Zhuo-ming Chen, Yun-Xia Wang, Wei-Xin Ling, Zhen Xing, and Han-Lin-Wei Xiao
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- 2013
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121. Designing a Fuzzy Classifier Using Genetic Algorithm and Application in Chinese Vowel Recognition.
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Wei-Xin Ling, Zhen Xing, Zhuo-ming Chen, and Da-Jun Tang
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- 2013
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122. Reduction of global natural gas hydrate (NGH) resource estimation and implications for the NGH development in the South China Sea
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Xiong-Qi Pang, Cheng-Zao Jia, Zhang-Xing Chen, He-Sheng Shi, Zhuo-Heng Chen, Tao Hu, Tong Wang, Zhi Xu, Xiao-Han Liu, Xing-Wen Zhang, En-Ze Wang, Zhuo-Ya Wu, and Bo Pang
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Geophysics ,Fuel Technology ,Geochemistry and Petrology ,Energy Engineering and Power Technology ,Economic Geology ,Geology ,Geotechnical Engineering and Engineering Geology - Published
- 2022
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123. Resistance traits and molecular characterization of multidrug-resistant Acinetobacter baumannii isolates from an intensive care unit of a tertiary hospital in Guangdong, southern China
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Yuanchun Huang, Jun Liu, Zhuo-Ran Chen, Ying-Kui Qiu, Mao-Zhang Fu, Qing Pan, Hui-Wu Guo, and Nai-Kei Wong
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Acinetobacter baumannii ,Microbiology (medical) ,Resistance (ecology) ,Microbial Sensitivity Tests ,Biology ,Intensive care unit ,Microbiology ,beta-Lactamases ,law.invention ,Anti-Bacterial Agents ,Tertiary Care Centers ,Intensive Care Units ,Aminoglycosides ,Southern china ,Bacterial Proteins ,Enterobacteriaceae ,law ,Drug Resistance, Multiple, Bacterial ,Humans ,Multidrug resistant Acinetobacter baumannii - Abstract
Purpose: This study aims to characterize antimicrobial resistance (AMR), with particular reference to carbapenems and aminoglycosides, in MDR A. baumannii isolates recovered from an intensive care unit in a tertiary hospital. Methods: A. baumannii (n = 95 strains) isolated from patients were subjected to antimicrobial susceptibility test (AST) by Vitek 2 Compact system to determine minimum inhibitory concentrations, followed by genotyping by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). Resistance genes of interest were PCR-amplified and sequenced. Results: All isolates were qualified as MDR, with a resistance rate of >80% to 8 antimicrobials tested. In terms of beta-lactamase detection, the blaOXA23 and blaTEM-1 genes were detected frequently at 92.63% and 91.58%, respectively. The metallo-β-lactamase genes blaIMP, blaVIM, and blaNDM were undetected. Aph (3’)-I was detected in 82 isolates (86.32%), making it the most prevalent aminoglycoside-modifying enzyme (AMEs) encoding gene. In addition, ant (3”)-I was detected at 30.53%, while 26.32% of the strains harboured an aac (6')-Ib gene. ERIC-PCR typing suggested moderate genetic diversity among the isolates, which might be organized into 10 distinct clusters, with Cluster A (n = 86 isolates or 90.53%) being a dominant cluster of epidemic clones.Conclusions: Substantial fractions of the A. baumannii strains prevailing in the ICU were MDR clones exhibiting extremely high resistance to carbapenems and aminoglycosides as monitored throughout the study period. They principally belonged to a single cluster of isolates carrying blaOXA23 and armA co-producing different AMEs genes.
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- 2022
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124. Modeling and analysis of magnetically coupled piezoelectric dual-beam with an annular potential energy function for broadband vibration energy harvesting
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Nan Shao, Zhuo Master Chen, Xian Wang, Chengxin Zhang, Jiawen Xu, Xiaosu Xu, and Ruqiang Yan
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Conventional piezoelectric cantilever-based vibration energy harvesters have narrow bandwidth. In this article, we develop a dual-beam piezoelectric energy harvester featuring an annular potential energy function that can harvest vibration energy over a wide spectrum under small amplitude excitations. The proposed harvester contains two conventional piezoelectric cantilevers placed orthogonal to each other which are coupled by repulsive magnetic force. We demonstrate analytically and numerically that a new annular potential energy function can be configurated with proper configuration. In the new annular stable state, the harvester can detour around the potential barrier rather than jump over it, yielding large-amplitude voltage outputs throughout a wide spectrum. Case studies were carried out and it is proved that the proposed annular stable harvester has a bandwidth of 3.9 Hz and a voltage output performance 3.01 times better than that of a conventional bistable one under excitations of 3 m/s2. The nonlinear dynamics of the proposed harvester are analyzed in detail.
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- 2023
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125. Spiritually integrated couple therapy
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Everett L. Worthington, Jennifer S. Ripley, Zhuo Job Chen, Vanessa M. Kent, and Elizabeth Loewer
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- 2023
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126. More spiritual than religious: Concurrent and longitudinal relations with personality traits, mystical experiences, and other individual characteristics
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Zhuo Job Chen, Richard G. Cowden, and Heinz Streib
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General Psychology - Abstract
People who self-identify as predominantly spiritual constitute a considerable and well-established part of the religious landscape in North America and Europe. Thus, further research is needed to document predictors, correlates, and outcomes associated with self-identifying primarily as a spiritual person. In the following set of studies, we contribute to some of these areas using data from German and United States adults. Study 1 (n = 3,491) used cross-sectional data to compare four religious/spiritual (R/S) self-identity groups—more religious than spiritual (MRTS), more spiritual than religious (MSTR), equally religious and spiritual (ERAS), and neither religious nor spiritual (NRNS)—on sociodemographic characteristics and a range of criterion variables (i.e., Big Five personality traits, psychological well-being, generativity, mystical experiences, religious schemata). In Study 2 (n = 751), we applied the analytic template for outcome-wide longitudinal designs to examine associations of the four R/S self-identifications with a range of subsequent outcomes (assessed approximately 3 years later) that were largely comparable to the criterion variables assessed in Study 1. The cross-sectional and longitudinal findings from these complementary studies provide further evidence of differences between these four categories of R/S self-identification, including strong evidence in both studies of an association between the MSTR self-identity and mysticism.
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- 2023
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127. Brain-gut-liver axis: Chronic psychological stress promotes liver injury and fibrosis via gut in rats
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Meng-Yang Xu, Can-Can Guo, Meng-Ying Li, Yu-Han Lou, Zhuo-Ran Chen, Bo-Wei Liu, and Ling Lan
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Microbiology (medical) ,Infectious Diseases ,Immunology ,Microbiology - Abstract
BackgroundThe effect of chronic psychological stress on hepatitis and liver fibrosis is concerned. However, its mechanism remains unclear. We investigated the effect and mechanism of chronic psychological stress in promoting liver injury and fibrosis through gut.MethodsSixty male SD rats were randomly assigned to 6 groups. Rat models of chronic psychological stress (4 weeks) and liver fibrosis (8 weeks) were established. The diversity of gut microbiota in intestinal feces, permeability of intestinal mucosa, pathologies of intestinal and liver tissues, collagen fibers, protein expressions of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor kappa β (NF-κβ), tumor necrosis factor α (TNF-α) and interleukin 1 (IL-1) in liver tissue, liver function and coagulation function in blood and lipopolysaccharide (LPS) in portal vein blood were detected and analyzed.ResultsThe diversities and abundances of gut microbiota were significant differences in rats among each group. The pathological lesions of intestinal and liver tissues, decreased expression of occludin protein in intestinal mucosa, deposition of collagen fibers and increased protein expression of TLR4, MyD88, NF-κβ, TNF-α and IL-1 in liver tissue, increased LPS level in portal vein blood, and abnormalities of liver function and coagulation function, were observed in rats exposed to chronic psychological stress or liver fibrosis. There were significant differences with normal rats. When the dual intervention factors of chronic psychological stress and liver fibrosis were superimposed, the above indicators were further aggravated.ConclusionChronic psychological stress promotes liver injury and fibrosis, depending on changes in the diversity of gut microbiota and increased intestinal permeability caused by psychological stress, LPS that enters liver and acts on TLR4, and active LPS-TLR4 pathway depend on MyD88. It demonstrates the possibility of existence of brain-gut-liver axis.
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- 2022
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128. Functional HPV-specific PD-1+ stem-like CD8 T cells in head and neck cancer
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Maria Cardenas, Mihir R. Patel, John Sidney, Alessandro Sette, Dong M. Shin, Mary Carrington, Tahseen H. Nasti, Haydn T. Kissick, Rafi Ahmed, Xu Wang, Zhuo Georgia Chen, Nataliya Prokhnevska, Andreas Wieland, Rebecca C. Obeng, Christopher C. Griffith, Se Jin Im, Nabil F. Saba, and Christiane S Eberhardt
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Multidisciplinary ,Squamous Cell Carcinoma of Head and Neck ,medicine.medical_treatment ,T cell ,Cellular differentiation ,Immunotherapy ,Biology ,Major histocompatibility complex ,Article ,Epitope ,medicine.anatomical_structure ,Antigen ,Head and Neck Neoplasms ,medicine ,Cancer research ,biology.protein ,Humans ,Cytotoxic T cell ,CD8 - Abstract
T cells are important in tumour immunity but a better understanding is needed of the differentiation of antigen-specific T cells in human cancer1,2. Here we studied CD8 T cells in patients with human papillomavirus (HPV)-positive head and neck cancer and identified several epitopes derived from HPV E2, E5 and E6 proteins that allowed us to analyse virus-specific CD8 T cells using major histocompatibility complex (MHC) class I tetramers. HPV-specific CD8 T cells expressed PD-1 and were detectable in the tumour at levels that ranged from 0.1% to 10% of tumour-infiltrating CD8 T lymphocytes (TILs) for a given epitope. Single-cell RNA-sequencing analyses of tetramer-sorted HPV-specific PD-1+ CD8 TILs revealed three transcriptionally distinct subsets. One subset expressed TCF7 and other genes associated with PD-1+ stem-like CD8 T cells that are critical for maintaining T cell responses in conditions of antigen persistence. The second subset expressed more effector molecules, representing a transitory cell population, and the third subset was characterized by a terminally differentiated gene signature. T cell receptor clonotypes were shared between the three subsets and pseudotime analysis suggested a hypothetical differentiation trajectory from stem-like to transitory to terminally differentiated cells. More notably, HPV-specific PD-1+TCF-1+ stem-like TILs proliferated and differentiated into more effector-like cells after in vitro stimulation with the cognate HPV peptide, whereas the more terminally differentiated cells did not proliferate. The presence of functional HPV-specific PD-1+TCF-1+CD45RO+ stem-like CD8 T cells with proliferative capacity shows that the cellular machinery to respond to PD-1 blockade exists in HPV-positive head and neck cancer, supporting the further investigation of PD-1 targeted therapies in this malignancy. Furthermore, HPV therapeutic vaccination efforts have focused on E6 and E7 proteins; our results suggest that E2 and E5 should also be considered for inclusion as vaccine antigens to elicit tumour-reactive CD8 T cell responses of maximal breadth. An analysis of human papillomavirus (HPV)-specific CD8 T cells in patients with head and neck cancer identifies functional PD-1+TCF-1+CD8 T cells in the tumour with implications for therapeutic vaccination and PD-1 directed immunotherapy.
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- 2021
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129. Potential roles of FAT1 somatic mutation in progression of head and neck cancer
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Zhuo G. Chen and Yong Teng
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Cancer Research ,Oncology - Published
- 2022
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130. Analytic thinking, religiosity, and defensiveness against secularism: Absence of causality
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Randle Aaron M. Villanueva, Zhuo Job Chen, and Yingjie Huang
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Social Psychology ,Religious studies ,Applied Psychology - Published
- 2022
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131. Network Analysis of Case Study Petra S.: A Mixed-methods Approach
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Zhuo Job Chen, Anika Steppacher, and Heinz Streib
- Abstract
Three waves of Petra’s interviews (her case study appears in Chapter 11) have been coded with the recently developed content coding scheme that applies over 150 prominent codes to describe each interview. These content codes are subject to quantitative analysis and visualization using the mathematical tools provided by network analysis. For each interview, content codes form a directed network of adjacent connections among the codes, and the edge weights reflect the frequency of each connection. In the analysis, node and network level statistics of centrality, connectivity, spread, subgroups, and homophily are offered to illustrate the node importance and various network structures. Inferential statistics use random graph modeling to test whether any of the network structures significantly differs from randomness such that they convey important information about how different codes connect with each other. Visualization of the trimmed networks provide further aid to understand the empirical connections of content codes. These quantitative analyses are enriched with interpretations of Petra’s life stories, thus providing both a panoramic view of the structure of her interviews, and a high-resolution view of some of their details. The use of network analysis to understand the structure of a qualitative interview opens doors to an array of mixed-methods research possibilities with the Faith Development Interview data and qualitative data alike.
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- 2022
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132. The Six Aspects of Faith Development in Longitudinal Analysis
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Zhuo Job Chen, Heinz Streib, and Ralph Hood
- Abstract
This chapter examines the meaning and development of the six aspects of faith development, perspective taking, social horizon, morality, locus of authority, world coherence, and symbolic function. In the existing literature on faith development, the aspects have been used to account for the variety of dimensions that are important for faith, and to warrant equal attention to every aspect when rating the faith development interview and calculating the total FDI score. The aspects have not being given individual treatment. The current study looks into the specific meaning and possible differences among these aspects. We first offer a theoretical overview of what these aspects measure. Then, with data of N = 75 individuals who completed three waves of faith development interviews, hierarchical linear models evidenced upward faith development only in perspective taking and social horizon, not in the other four aspects. A rigorous outcome-wide analysis explored the possible causes of faith development using self-report personality measures. Most importantly, self-acceptance appeared to be a consistent inhibitor of faith development. There were some associations of neuroticism and the religious schemata of truth of text and teachings and of xenosophia interreligious dialogue with faith development. Overall, the associations of personality measures with faith development aspects were weak and not uniform.
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- 2022
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133. Chinese Psychology of Religion: Eastern Traditions
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Zhuo Job Chen
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This chapter will focus on Eastern traditions including Buddhism, Daoism, Confucianism/syncretism, and folk religions. Buddhism, being originally from India, has been a staple religion among both Han people devoted to various schools of Mahayana Buddhism, and Tibetans, including adjacent ethnicities such as Mongolians and Tu, devoted to various schools of Vajrayana Buddhism. Daoism, by contrast, was originally a Han religion and has arguably shaped, as well as being shaped by, the fundamental Chinese way of thinking. Confucianism is as much a national philosophy as a religion and, syncretizing with Buddhism and Daoism, its influence ranges from defining what an ideal person is to how a society should be governed. The picture of folk religions is much more complex – multiple layers of folk beliefs and practices permeate Chinese lives running the gamut of fengshui, ancestor worshiping, and believing in gods without any official affiliation. This chapter selects key literature from both mainland China (database for Chinese publications is at cnki.net) and Taiwan (airitilibrary.com). Many of the literature reviewed are published in Chinese which the authors read as native speakers. It is our intention to present an insider’s view of what Chinese psychology of religion is like from within its cultural context.
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- 2022
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134. Lack of evolutionary convergence in multiple primary lung cancer suggests insufficient specificity of personalized therapy
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Hua Cheng, Ziyan Guo, Xiaoyu Zhang, Xiao-Jin Wang, Zizhang Li, Wen-Wen Huo, Hong-Cheng Zhong, Xiao-Jian Li, Xiang-Wen Wu, Wen-Hao Li, Zhuo-Wen Chen, Tian-Chi Wu, Xiang-Feng Gan, Bei-Long Zhong, Vassily A. Lyubetsky, Leonid Yu Rusin, Junnan Yang, Qiyi Zhao, Qing-Dong Cao, and Jian-Rong Yang
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Genetics ,Molecular Biology - Abstract
Multiple primary lung cancer (MPLC) is an increasingly prevalent subtype of lung cancer. According to recent genomic studies, the different lesions of a single MPLC patient exhibit functional similarities that may reflect evolutionary convergence. We performed whole-exome sequencing for a unique cohort of MPLC patients with multiple samples from each lesion found. Using our own and other relevant public data, evolutionary tree reconstruction revealed that cancer driver gene mutations occurred at the early trunk, indicating evolutionary contingency rather than adaptive convergence. Additionally, tumors from the same MPLC patient were as genetically diverse as those from different patients, while within-tumor genetic heterogeneity was significantly lower. Furthermore, the aberrant molecular functions enriched in mutated genes for a sample showed a strong overlap with other samples from the same tumor, but not with samples from other tumors or other patients. Overall, there was no evidence of adaptive convergence during the evolution of MPLC. Most importantly, the similar between-tumor diversity and between-patient diversity suggests that personalized therapies may not adequately account for the genetic diversity among different tumors in an MPLC patient. To fully exploit the strategic value of precision medicine, targeted therapies should be designed and delivered on a per-lesion basis.
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- 2022
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135. Correlation analysis between physicians' evaluations of doctor–patient relationship and their preferences for shared decision-making in China
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Zhuo-Ran, Chen, Li, Zhang, Ya-Wei, Chen, Meng-Yang, Xu, Hang, Jia, Meng-Ying, Li, Yu-Han, Lou, and Ling, Lan
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Psychiatry and Mental health - Abstract
Shared decision-making (SDM) is a scientific and reasonable decision-making model. However, whether physicians choose SDM is usually influenced by many factors. It is not clear whether the strained doctor–patient relationship will affect physicians' willingness to choose SDM. Through a survey by questionnaire, 304 physicians' evaluations of doctor–patient relationship (DPR) were quantified by the difficult DPR questionnaire-8. Their preferences for SDM and the reasons were also evaluated. The correlation between physicians' evaluations of DPR and their preferences for SDM were analyzed. 84.5% physicians perceived DPR as poor or strained, 53.3% physicians preferred SDM, mainly because of the influences of medical ethics and social desirability bias. Their preferences for SDM were not significantly correlated with their evaluations of DPR (P > 0.05). Physicians with different evaluations of DPR (good, poor, and strained) all had similar preferences for SDM (42.6, 56.4, and 42.9%), with no significant difference (P > 0.05). There was no correlation between physicians' evaluations of DPR and their preferences for SDM. Physicians' evaluations of poor DPR did not affect their preferences for SDM. This may be influenced by the medical ethics and social desirability bias.
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- 2022
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136. Temporal convolution network‐based time frequency domain integrated model of multiple arch dam deformation and quantification of the load impact
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Xin Wu, Dong‐Jian Zheng, Yong‐Tao Liu, Zhuo‐Yan Chen, and Xing‐Qiao Chen
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Mechanics of Materials ,Building and Construction ,Civil and Structural Engineering - Published
- 2022
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137. Adapting Positively to Trauma: Associations of Posttraumatic Growth, Wisdom, and Virtues in Survivors of Civil War
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Mihaly Csikszentmihalyi, Everett L. Worthington, Richard G. Cowden, Andrea Ortega Bechara, Richard G. Tedeschi, and Zhuo Job Chen
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Psychiatry and Mental health ,Clinical Psychology ,Spanish Civil War ,Posttraumatic growth ,Criminology ,Psychology ,Health Professions (miscellaneous) - Abstract
A growing body of research is emerging on the role of adaptive resources and processes that support positive transformation in the aftermath of adversity. However, few studies in this strand have f...
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- 2021
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138. In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation
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Hou-Fu Xia, Zi-Li Yu, Hai-Ming Liu, Yi-Fang Zhao, Min Wu, Fan Miao, Yi Zhao, Zhuo-Kun Chen, and Gang Chen
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In situ ,Chemistry ,viruses ,Cell ,Kinetics ,virus diseases ,respiratory system ,Extracellular vesicles ,In vitro ,Analytical Chemistry ,Extracellular Vesicles ,Mice ,medicine.anatomical_structure ,Membrane ,In vivo ,Biotinylation ,medicine ,Biophysics ,Animals ,Biomarkers - Abstract
Circulating small extracellular vesicles (sEVs) are naturally occurring nanosized membrane vesicles that convey bioactive molecules between cells. Conventionally, to evaluate their behaviors in vivo, circulating sEVs have to be isolated from the bloodstream, then labeled with imaging materials in vitro, and finally injected back into the circulation of animals for subsequent detection. The tedious isolation-labeling-reinfusion procedures might have an undesirable influence on the natural properties of circulating sEVs, thereby changing their behaviors and the detected kinetics in vivo. Herein, we proposed an in situ biotinylation strategy to directly label circulating sEVs with intravenously injected DSPE-PEG-Biotin, aiming to evaluate the in vivo kinetics of circulating sEVs more biofriendly and accurately. Such an analysis strategy is free of isolation-labeling-reinfusion procedures and has no unfavorable influence on the natural behaviors of sEVs. The results showed that the lifetime of generic circulating sEVs in mice was around 3 days. Furthermore, we, for the first time, revealed the distinct in vivo kinetics of circulating sEV subpopulations with different cell sources, among which erythrocyte-derived sEVs showed the longest lifespan. Moreover, compared with circulating sEVs in situ or used as autograft, circulating sEVs used as allograft had the shortest lifetime. In addition, the in situ biotinylation strategy also provides a way for the enrichment of biotinylated circulating sEVs. In summary, this study provides a novel strategy for in situ labeling of circulating sEVs, which would facilitate the accurate characterization of their kinetics in vivo, thereby accelerating their future application as biomarkers and theranositic vectors.
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- 2021
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139. Spiritual Experiences in Soulmate Relationships: Qualitative and Network Analysis of the Mystical Bond
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Janki Patel and Zhuo Job Chen
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Religious experience ,Bond ,Religious studies ,Context (language use) ,Psychology ,Individual level ,General Psychology ,Mysticism ,Network analysis ,Epistemology - Abstract
Mysticism has so far been studied primarily at an individual level and often in the context of a religious tradition. However, one can and often does acquire strong spiritual experience in a mundan...
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- 2021
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140. The Proteomic Landscape of Growth Factor Signaling Networks Associated with FAT1 Mutations in Head and Neck Cancers
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Carter Van Waes, Zhengjia Chen, Dongsheng Wang, Chao Zhang, Jianhong Chen, Zhong Chen, Jieqi Tu, Nabil F. Saba, and Zhuo Georgia Chen
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Cancer Research ,Mutation ,Cancer ,Biology ,medicine.disease ,medicine.disease_cause ,Head and neck squamous-cell carcinoma ,stomatognathic diseases ,Oncology ,ErbB ,Gene expression ,Cancer research ,medicine ,Neuregulin ,Gene ,FAT1 - Abstract
FAT1 is frequently mutated in head and neck squamous cell carcinoma (HNSCC), but the biological and clinical effects of FAT1 mutations in HNSCC remain to be fully elucidated. We investigated the landscape of altered protein and gene expression associated with FAT1 mutations and clinical outcomes of patients with HNSCC. FAT1 mutation was stratified with clinical information from The Cancer Genome Atlas HNSCC databases with more than 200 proteins or phosphorylated sites. FAT1 mutation was significantly more prevalent among HPV(−), female, and older patients and was enriched in oral, larynx, and hypopharynx primary tumors. FAT1 mutation was also significantly associated with lower FAT1 gene expression and increased protein expression of HER3_pY1289, IRS1, and CAVEOLIN1. From an independent International Cancer Genome Consortium dataset, FAT1 mutation in oral cancer co-occurred with top mutated genes TP53 and CASP8. Poorer overall survival or progression-free survival was observed in patients with FAT1 mutation or altered HER3_pY1289, IRS1, or CAVEOLIN1. Pathway analysis revealed dominant ERBB/neuregulin pathways linked to FAT1 mutations in HNSCC, and protein signature panels uncovered the heterogeneity of patient subgroups. Decreased pEGFR, pHER2, and pERK and upregulated pHER3 and HER3 proteins were observed in two FAT1 knockout HNSCC cell lines, supporting that FAT1 alterations lead to altered EGFR/ERBB signaling. In squamous cancers of the lung and cervix, a strong association of FAT1 and EGFR gene expressions was identified. Collectively, these results suggest that alteration of FAT1 appears to involve mostly HPV(−) HNSCC and may contribute to resistance to EGFR-targeted therapy. Significance: Integrative bioinformatics and statistical analyses reveal a panel of genes and proteins associated with FAT1 mutation in HNSCC, providing important insights into prospective clinical investigations with targeted therapies.
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- 2021
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141. Which patents to use as loan collaterals? The role of newness of patents' external technology linkage
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Yuandi Wang, Yan Anthea Zhang, and Zhuo Emma Chen
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Loan ,law ,Strategy and Management ,Financial system ,Linkage (mechanical) ,Business ,Business and International Management ,law.invention - Published
- 2021
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142. Protein structure dynamics by crosslinking mass spectrometry
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Zhuo Angel Chen and Juri Rappsilber
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Structural Biology ,Molecular Biology - Published
- 2023
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143. Deformable registration of histological cancer margins to gross hyperspectral images using demons.
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Martin T. Halicek, James V. Little, Xu Wang, Zhuo Georgia Chen, Mihir Patel, Christopher C. Griffith, Mark W. El-Deiry, Nabil F. Saba, Amy Y. Chen, and Baowei Fei
- Published
- 2018
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144. The diverse functions of FAT1 in cancer progression: good, bad, or ugly?
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Zhuo Georgia Chen, Nabil F. Saba, and Yong Teng
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Cancer Research ,Epithelial-Mesenchymal Transition ,Oncology ,Head and Neck Neoplasms ,Squamous Cell Carcinoma of Head and Neck ,Humans ,Cadherins ,Signal Transduction - Abstract
FAT atypical cadherin 1 (FAT1) is among the most frequently mutated genes in many types of cancer. Its highest mutation rate is found in head and neck squamous cell carcinoma (HNSCC), in which FAT1 is the second most frequently mutated gene. Thus, FAT1 has great potential to serve as a target or prognostic biomarker in cancer treatment. FAT1 encodes a member of the cadherin-like protein family. Under normal physiological conditions, FAT1 serves as a molecular “brake” on mitochondrial respiration and acts as a receptor for a signaling pathway regulating cell–cell contact interaction and planar cell polarity. In many cancers, loss of FAT1 function promotes epithelial-mesenchymal transition (EMT) and the formation of cancer initiation/stem-like cells. However, in some types of cancer, overexpression of FAT1 leads to EMT. The roles of FAT1 in cancer progression, which seems to be cancer-type specific, have not been clarified. To further study the function of FAT1 in cancers, this review summarizes recent relevant literature regarding this protein. In addition to phenotypic alterations due to FAT1 mutations, several signaling pathways and tumor immune systems known or proposed to be regulated by this protein are presented. The potential impact of detecting or targeting FAT1 mutations on cancer treatment is also prospectively discussed.
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- 2022
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145. The Effect of Electroacupuncture Preconditioning on Regional Cerebral Oxygen Saturation Levels in Elderly Patients with Diabetes
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Jia-Qi Ning, Jian-Sheng Luo, Ling-Ling Ding, Yu-Hong Guo, Zhuo-Ya Chen, Qi Wang, and Rui-Ling Zhou
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Pharmacology ,Internal Medicine ,Targets and Therapy [Diabetes, Metabolic Syndrome and Obesity] - Abstract
Jia-Qi Ning,1,2 Jian-Sheng Luo,1,2 Ling-Ling Ding,2 Yu-Hong Guo,3 Zhuo-Ya Chen,2 Qi Wang,2 Rui-Ling Zhou2 1Department of Anesthesiology, Capital Medical University, Beijing, 100069, Peopleâs Republic of China; 2Department of Anesthesiology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, 100010, Peopleâs Republic of China; 3Department of Emergency, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, 100010, Peopleâs Republic of ChinaCorrespondence: Ling-Ling Ding, Department of Anesthesiology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing, 100010, Peopleâs Republic of China, Tel/Fax +86 1087906647, Email dignlilbc@outlook.com; dinglingling301@126.comObjective: This study aimed to evaluate the effect of electroacupuncture preconditioning on regional cerebral oxygen saturation (rSO2) levels in elderly patients with diabetes.Methods: Forty patients undergoing elective diabetic foot surgery were enrolled in this study. All patients were aged 65 years and above and weighed 45â 75 kg. All were characterized as class II or III according to the American Society of Anesthesiologistsâ physical status classification system. Patients were divided randomly into an electroacupuncture group (group E) and a control group (group C); both groups comprised 20 patients. In group E, the DU20 (Baihui), DU24 (Shenting), and EX-HN1 (Sishencong) acupoints were selected for electroacupuncture 30 min prior to administering anesthesia, while in group C, patients underwent routine anesthesia without electroacupuncture. The patients in both groups were anesthetized using a sciatic nerve block. The number of cases with increased or decreased regional oxygen saturation (rSO2) compared with the baseline as well as rSO2 variability in the two groups were recorded and compared.Results: There was no significant difference in the preoperative rSO2 values between the two groups (54.4 ± 4.8 (L), 53.9 ± 5.2 (R) [group C] vs 54.1 ± 5.2 (L), 54.5 ± 4.6 (R)[group E]). Compared with group C, the rSO2 in group E increased (50.3 ± 3.9 [group C] vs 58.4 ± 3.2[group E]), and this difference was statistically significant (P < 0.001).Conclusion: Electroacupuncture stimulation can increase rSO2 levels in patients with diabetes.Clinical Registration Number: ChiCTR2100048783 (http://www.chictr.org.cn).Keywords: electroacupuncture, preconditioning, regional cerebral oxygen saturation, diabetes
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- 2022
146. Call and response: A six-wave study of bidirectional links between religiosity and spirituality among Pakistani Muslims during Ramadan
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Zhuo Job Chen, Ziasma Khan, Richard G. Cowden, Roman Palitsky, and Yingjie Huang
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Social Psychology ,Religious studies ,Applied Psychology - Published
- 2022
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147. Consonant recognition of dysarthria Based on Wavelet Transform and Fuzzy Support Vector Machines.
- Author
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Zhuo-ming Chen, Wei-Xin Ling, Jian-hui Zhao, and Tao-tao Yao
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- 2011
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148. Shulin packages axonemal outer dynein arms for ciliary targeting
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Andrew P. Carter, Ferdos Abid Ali, G.R. Mali, Juri Rappsilber, Jérôme Boulanger, Clinton K. Lau, Jonathan D. Howe, Farida Begum, Mark Skehel, and Zhuo A. Chen
- Subjects
Models, Molecular ,Cytoplasm ,Protein Conformation ,Movement ,Dynein ,Protozoan Proteins ,Plasma protein binding ,Flagellum ,Microtubules ,Article ,Tetrahymena thermophila ,Protein Domains ,Ciliogenesis ,Image Processing, Computer-Assisted ,Cilia ,Ciliate ,Multidisciplinary ,biology ,Chemistry ,Cilium ,Cryoelectron Microscopy ,Tetrahymena ,Axonemal Dyneins ,biology.organism_classification ,Cell biology ,Gene Knockdown Techniques ,Protein Binding - Abstract
Ciliary motors locked closed by Shulin Motile cilia and flagella are vital cellular organelles with functions that include setting up the left-right body axis, clearing airways of mucus, and driving single-cell movements. Cilia beating is powered by arrays of dynein motors, the key force generators being the outer dynein arm (ODA) complexes. Using the protozoan Tetrahymena , Mali et al. identified a factor, which they name Shulin, that binds newly synthesized ODAs. Cryo–electron microscopy revealed how Shulin locks the dynein motors together by shutting off motor activity and facilitating delivery of ODAs from the cytoplasm to their final position in the cilia. Science , this issue p. 910
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- 2021
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149. A Common Drain Operational Amplifier Using Positive Feedback Integrated by Metal-Oxide TFTs
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Wen-Xing Xu, Jianhua Zou, Junbiao Peng, Xiang-Lin Mei, Lei Zhou, Miao Xu, Wei-Jing Wu, Lei Wang, Yu-Rong Liu, and Zhuo-Jia Chen
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Common drain ,Materials science ,Transconductance ,Phase margin ,Topology (electrical circuits) ,02 engineering and technology ,01 natural sciences ,law.invention ,law ,operational amplifier (OPAMP) ,0103 physical sciences ,0202 electrical engineering, electronic engineering, information engineering ,Electrical and Electronic Engineering ,010302 applied physics ,business.industry ,020208 electrical & electronic engineering ,Transistor ,positive feedback ,TK1-9971 ,Electronic, Optical and Magnetic Materials ,Thin-film transistor ,indium-zinc-oxide (IZO) thin-film transistors (TFTs) ,Operational amplifier ,common-drain ,Optoelectronics ,Cross-coupled ,Electrical engineering. Electronics. Nuclear engineering ,business ,Biotechnology ,Voltage - Abstract
This paper presents a common-drain-based operational amplifier (OPAMP) fabricated by mono n-type indium-zinc-oxide (IZO) thin-film transistors (TFTs). Positive feedback technology is employed to the load TFTs by cross-coupled connection in order to boost the voltage gain of the common-drain differential pair. The OPAMP exhibits an open-loop voltage gain (Av) of 27 dB over a −3 dB bandwidth (BW) of 8.4 kHz at a DC supply voltage of 10 V. The measured unity-gain frequency (UGF), phase margin (PM) and DC power consumption are 119.4 kHz, 36° and 0.96 mW, respectively. Moreover, the chip area of the proposed OPAMP is as small as 0.37 mm $\times0.3$ mm since this concise topology needs only 10 TFTs.
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- 2021
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150. Looking Forward to the Future: Visual Prospects and Optimism
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Kevin Nute and Zhuo Job Chen
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Optimism ,media_common.quotation_subject ,Positive economics ,Psychology ,media_common - Published
- 2021
- Full Text
- View/download PDF
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