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External obstacle detection is a significant task in transmission line inspection and is related to the safe operation of the power transmission grid. In recent years, unmanned aerial vehicles (UAVs) equipped with different devices have been widely used for transmission line inspection. However, because of the complex environment of transmission lines and weak power line textures in the obtained images, most existing methods and systems cannot meet the requirements for real-time and high-accuracy external obstacle detection of transmission lines. In this paper, a novel, to the best of our knowledge, UAV system integrated trinocular vision technology with remote sensing is developed to achieve better external obstacle detection of transmission lines in real time, which is composed of a DJ-Innovations (DJI) UAV equipped with a global positioning system (GPS), angle sensors, trinocular vision including three visible cameras with the same parameters, and a small processor with a pre-implanted software algorithm. In this paper, a new method for external obstacle detection of transmission lines is proposed to satisfy the requirements for real-time and high-accuracy practical inspection applications. First, the original trinocular images need to be rectified. Then, the rectified trinocular images are adopted to achieve three-dimensional reconstruction of power lines. Finally, based on trinocular vision, bag of feature, and GPS, the clearance distance measurement, obstacle classification, and obstacle location are realized. Experimental tests on 220 kV transmission lines reveal that our proposed system can be applied in practical inspection environments and has good performance.

We study how management practices shape export performance using matched production-trade-management data for Chinese and American firms and a randomized control trial in India. Better-managed firms are more likely to export, sell more products to more destinations, and earn higher export revenues and profits. They export higher-quality products at higher prices and lower quality-adjusted prices. They import a wider range of inputs and inputs of higher quality and price, from more advanced countries. We rationalize these patterns with a heterogeneous-firm model in which effective management improves performance by raising production efficiency and quality capacity.

The soaring installation of volatile Photovoltaic (PV) source in both utility-scale and distributed scale renders power systems susceptible to solar dynamics. Automatic generation controls (AGC) parameters are normally determined without any further adaptation, making it a challenge for multi-area interconnected power systems balancing the frequency with the PV integration. Considering the synchronous updating issue of the multi-area power system, an asynchronous tuning method is proposed in this paper to help power systems refine their optimal AGC parameters for better power transfer control. The phasor measurement units (PMU) based data has been considered for the proposed tuning method as well as the other data format. A performance study with manual, two-step tuned and asynchronous tuned AGCs has been conducted on a five-area power system in real-time digital simulator (RTDS). Results present that the asynchronous method provides optimal AGCs for the test five-area power system with PV stations. Results also demonstrate a better frequency and tie-line power response performance with the proposed method.

Background There are few studies that characterize sex-related differences in HIV outcomes among adolescents and young adults (AYA) 15–24 years of age. Methods We conducted a retrospective cohort study among AYA who enrolled in a comprehensive HIV program in Mozambique between 2012–2016. We assessed patients by sex and pregnancy/lactation status, comparing time to combination antiretroviral therapy (ART) initiation using Cox proportional hazard models. We employed multivariable logistic regression to investigate pre- and post-ART retention. Patients were defined as ‘retained pre-ART’ if they attended at least 3 of 4 required visits or started ART in the 6 months after enrollment, and ‘retained post-ART’ if they had any ART pickup or clinical visit during the last 90 days of the one-year follow-up period. Results Of 47,702 patients in the cohort, 81% (n = 38,511) were female and 19% (n = 9,191) were male. Of the females, 57% (n = 21,770) were non-pregnant and non-lactating (NPNL) and 43% (n = 16,741) were pregnant or lactating (PL). PL (aHR 2.64, 95%CI:2.47–2.81) and NPNL females (aHR 1.36, 95%CI:1.30–1.42) were more likely to initiate ART than males. PL females had higher odds of pre-ART retention in care (aOR 3.56, 95%CI: 3.30–3.84), as did NPNL females (aOR 1.71, 95%CI: 1.62–1.81), compared to males. This was also true for retention post-ART initiation, with higher odds for both PL (aOR 1.78, 95%CI:1.63–1.94) and NPNL females (aOR 1.50, 95%CI:1.35–1.65) compared to males. Conclusions PL females were most likely to initiate ART and remain in care post-ART in this AYA cohort, likely reflecting expansion of Option B+. Despite pregnancy and policy driven factors, we observed important sex-related disparities in this cohort. NPNL females were more likely to initiate ART and be retained in care before and after ART initiation than males. These data suggest that young males need targeted interventions to improve these important care continuum outcomes.

We previously reported that cell-free heme (CFH) is increased in the plasma of patients with acute and chronic lung injury and causes pulmonary edema in animal model of acute respiratory distress syndrome (ARDS) post inhalation of halogen gas. However, the mechanisms by which CFH causes pulmonary edema are unclear. Herein we report for the first time the presence of CFH and chlorinated lipids (formed by the interaction of halogen gas, Cl2, with plasmalogens) in the plasma of patients and mice exposed to Cl2gas.Ex vivoincubation of red blood cells (RBC) with halogenated lipids caused oxidative damage to RBC cytoskeletal protein spectrin, resulting in hemolysis and release of CFH. A single intramuscular injection of the heme-scavenging protein hemopexin (4 µg/kg body weight) in mice, one hour post halogen exposure, reversed RBC fragility and decreased CFH levels to those of air controls. Patch clamp and short circuit current measurements revealed that CFH inhibited the activity of amiloride-sensitive (ENaC) and cation sodium (Na+) channels in mouse alveolar cells and trans-epithelial Na+transport across human airway cells with EC50of 125 nM and 500 nM, respectively. Molecular modeling identified 22 putative heme-docking sites on ENaC (energy of binding range: 86-1563 kJ/mol) with at least 2 sites within its narrow transmembrane pore, potentially capable of blocking Na+transport across the channel. In conclusion, results suggested that CFH mediated inhibition of ENaC activity may be responsible for pulmonary edema post inhalation injury.

We investigated the mechanisms involved in the development of airway hyperresponsiveness (AHR) following exposure of mice to halogens. Male mice (C57BL/6; 20–25 g) exposed to either bromine (Br2) or Cl2(600 or 400 ppm, respectively, for 30 min) developed AHR 24 h after exposure. Nifedipine (5 mg/kg body wt; an L-type calcium channel blocker), administered subcutaneously after Br2or Cl2exposure, produced higher AHR compared with Br2or Cl2alone. In contrast, diltiazem (5 mg/kg body wt; a nondihydropyridine L-type calcium channel blocker) decreased AHR to control (air) values. Exposure of immortalized human airway smooth muscle cells (hASMC) to Br2resulted in membrane potential depolarization ( VmAir: 62 ± 3 mV; 3 h post Br2:−45 ± 5 mV; means ± 1 SE; P < 0.001), increased intracellular [Ca2+]i, and increased expression of the calcium-sensing receptor (Ca-SR) protein. Treatment of hASMC with a siRNA against Ca-SR significantly inhibited the Br2and nifedipine-induced Vmdepolarization and [Ca2+]iincrease. Intranasal administration of an antagonist to Ca-SR in mice postexposure to Br2reversed the effects of Br2and nifedipine on AHR. Incubation of hASMC with low-molecular-weight hyaluronan (LMW-HA), generated by exposing high-molecular-weight hyaluronan (HMW-HA) to Br2, caused Vmdepolarization, [Ca2+]iincrease, and Ca-SR expression to a similar extent as exposure to Br2and Cl2. The addition of HMW-HA to cells or mice exposed to Br2, Cl2, or LMW-HA reversed these effects in vitro and improved AHR in vivo. We conclude that detrimental effects of halogen exposure on AHR are mediated via activation of the Ca-SR by LMW-HA.

BackgroundGastric cancer is as the gastrointestinal issue, the second most death-cause complication worldwide. The survival rate of gastric cancer is lesser due to diagnosing it at the advanced stage. SRY-box containing gene 17 (SOX-17) expression and methylation participate a crucial role in the gastric cancer. MethodsIn this research, capture probe modified interdigitated electrode was used to quantify the SOX-17 gene target sequence. To improve the detection, IDE sensing surface was physically-modified by silica-alumina (Si-Al) nanocomposite. Through the biotin-streptavidin strategy, capture probe was immobilized on the surface and complemented by the target sequence. ResultsDoubled the level of capture probe immobilization was noticed on the Si-Al modified surface. From 1 aM concentration of target sequence was detected in the presence of Si-Al nanocomposite, while it was reached 10 aM in the absence, shows ten-folds difference. In addition, higher level of current changes was registered with all the concentrations of target sequence. Control experiments with single, triple and complementary sequences of target were done and there is no significant changes in current were recorded in the substituted sequences, representing the specific detection of target SOX-17 gene sequence. ConclusionThe detection method is shown with nanocomposite-modified IDE surface helps to recognize the gastric cancer effectively.

Acute lung injury (ALI) is a severe disease with high morbidity and mortality, and is characterized by devastating inflammation of the lung and increased production of reactive oxygen species (ROS). Recent studies have indicated that fatty acid binding protein (FABP4) is important in the regulation of inflammation. However, the role of FABP4 in sepsis-related ALI, and the specific mechanism of action have not been examined. In vitro, the exposure of human alveolar epithelial A549 cells to lipopolysaccharide (LPS) and recombinant FABP4 (hrFABP4) resulted in the production of pro-inflammatory cytokines, inflammatory cytokines, and ROS, while these changes were ameliorated by pretreatment with the FABP4 inhibitor BMS309403 and FABP4 siRNA. Sequentially, treatment of A549 cells with N-acetylcysteine (NAC) significantly attenuated LPS and hrFABP4-induced the generation of ROS and the release of inflammatory cytokines. In vivo, a cecal ligation and puncture (CLP)-induced ALI murine model was successfully established. Then, the mice were treated with FABP4 inhibitor BMS309403. The results showed treatment with BMS309403 improved the survival rate of CLP-induced ALI mice, and prevented lung inflammation, histopathological changes, and increase of FABP4 induced by CLP. These data indicate that FABP4 plays an important role in lung inflammation of sepsis-induced ALI. Blockade of FABP4 signaling exhibits a protective effect in a CLP-induced ALI mouse model, and in A549 cell LPS specifically induces enhanced expression of FABP4, which then causes inflammatory cytokine production by elevating the ROS level.

This paper presents a chaotic particle swarm optimization (CPSO) algorithm combined with data mining method for transient stability preventive control. The data mining method is utilized to approximate the security region considering transient stability. Therefore, the application effects of different input features and data-mining classifiers are compared first. Then, a two-stage support vector machine (SVM) approach is proposed to generate two models, including a linear SVM model with controllable features provides preventive adjustment rules, and a more accurate SVM model to approximate the actual security region. Finally, the CPSO in combination with the two-stage SVM is proposed to calculate the optimal preventive control strategies. Comprehensive studies are conducted on a 16-machine 68-bus system and 48-machine 140-bus system to verify the effectiveness.

Information regarding the toxic effects of cadmium (Cd) adsorbed by nano-hydroxyapatite (NHAP-Cd) on the growth of crop plants remain limited. We investigated the mechanism of NHAP-Cd (diameters, 20 and 40 nm; NHAP 20 -Cd and NHAP 40 -Cd, respectively) phytotoxicity. Rice seedlings treated with Cd and NHAP 20 -Cd showed more severe growth retardation compared to those treated with NHAP 40 -Cd, for the same Cd concentration. Transmission electron microscopy revealed NHAP in the seedlings. The nanoparticles entered the rice seedlings with no Cd 2+ signals in the NHAP treatments compared to −0.47 pmol cm −2 s −1 of Cd 2+ fluxes in the Cd treatment. The higher Cd 2+ content in the leaves and mesocotyl of NHAP 20 -Cd-treated rice seedlings suggested that smaller NHAP-Cd can translocate easily to the aboveground parts. Further, NHAP-Cd increased oxidative stress, which was determined as catalase activity changes in this study. Thus, NHAP-Cd particles in the growth medium can be transported to rice seedlings and cause toxicity.

Pipeline is an important transportation facility in the oil and gas industries. But cracks inevitably appear in the pipe body due to various factors. Mechanical analysis of cracked pipe structures based on local flexibility has received increased attention in the last three decades. However, few reports exist on the local flexibility of pipes with an arbitrary angled crack. In this paper, the general solution of the local flexibility equations of a pipe with a part through-crack subjected to axial force, shearing force, and bending moment is deduced with respect to an arbitrary angled crack. The proposed equations consider the influence of the crack orientation on the local flexibility coefficient. An adaptive Simpson method is used to calculate the local flexibility coefficients of a cracked pipe. The results of testing by Naniwadekar’s and Authors’ are used to validate the proposed method. The results demonstrate that the proposed method is accurate for calculating local flexibility and can be applied for vibration analysis in a pipe-like structure.

Severe asthma with fungal sensitization (SAFS) defines a subset of human asthmatics with allergy to 1 or more fungal species and difficult-to-control asthma. We have previously reported that human asthmatics sensitized to fungi have worse lung function and a higher degree of atopy, which was associated with higher IL-1 receptor antagonist (IL-1RA) levels in bronchoalveolar lavage fluid. IL-1RA further demonstrated a significant negative association with bronchial hyperresponsiveness to methacholine. Here, we show that IL-1α and IL-1β are elevated in both bronchoalveolar lavage fluid and sputum from human asthmatics sensitized to fungi, implicating an association with IL-1α, IL-1β, or IL-1RA in fungal asthma severity. In an experimental model of fungal-associated allergic airway inflammation, we demonstrate that IL-1R1 signaling promotes type 1 (IFN-γ, CXCL9, CXCL10) and type 17 (IL-17A, IL-22) responses that were associated with neutrophilic inflammation and increased airway hyperreactivity. Each of these were exacerbated in the absence of IL-1RA. Administration of human recombinant IL-1RA (Kineret/anakinra) during fungal-associated allergic airway inflammation improved airway hyperreactivity and lowered type 1 and type 17 responses. Taken together, these data suggest that IL-1R1 signaling contributes to fungal asthma severity via immunopathogenic type 1 and type 17 responses and can be targeted for improving allergic asthma severity.

Influenza is a contagious respiratory virus that infects epithelial cells throughout the respiratory tract. The viral infection is facilitated by the attachment of the viral hemagglutinin to the si...

Infants have a higher risk of developing allergic asthma than adults. However, the underlying mechanism remains unknown. We show here that sensitization of mice with house-dust mites (HDMs) in the presence of low-dose lipopolysaccharide (LPS) prevented T helper 2 (Th2) cell allergic responses in adult, but not infant, mice. Mechanistically, adult CD11b + migratory dendritic cells (mDCs) upregulated the transcription factor T-bet in response to tumor necrosis factor-α (TNF-α), which was rapidly induced after HDM + LPS sensitization. Consequently, adult CD11b+ mDCs produced interleukin-12 (IL-12), which prevented Th2 cell development by promoting T-bet upregulation in responding T cells. Conversely, infants failed to induce TNF-α after HDM + LPS sensitization. Therefore, CD11b+ mDCs failed to upregulate T-bet and did not secrete IL-12 and Th2 cell responses normally developed in infant mice. Thus, the availability of TNF-α dictates the ability of CD11b+ mDCs to suppress allergic Th2-cell responses upon dose-dependent endotoxin sensitization and is a key mediator governing susceptibility to allergic airway inflammation in infant mice. [The above abstract is a summary from our recently published paper under the same title – Immunity; published: Jan 8, 2019 DOI: https://doi.org/10.1016/j.immuni.2018.11.012]

With the development of social economy at this stage, the construction industry has also increased certain progress, in the construction engineering construction, construction safety management is very important. Compared with the traditional construction safety management, BIM Technology can find the hidden danger in the construction project in time, and check the potential safety hazard in time, so as to ensure the safety of construction. In this paper, BIM Technology is used to manage the safety management of construction site in time and space, and the fall protection control based on BIM is studied. Hope to be able to provide a certain reference and reference for future related project safety management, so as to ensure that the overall construction safety management ability and quality of the construction project can meet the requirements to the greatest extent.

We show using detailed firm-level Chinese data that, among small exporters, firms selling to a more diversified set of countries have more volatile exports, while the opposite holds among large exporters. This result, which stands in marked contrast to standard portfolio theory for small exporters, is robust to a wide array of specifications and controls. Our theoretical explanation for these observations rests on the presence of fixed costs of exports per destination and short-run demand shocks. In this setup, the volatility of a firm’s exports depends not only on the diversification of its destination portfolio but also on whether it exports permanently to all markets. Among small exporters, a more diversified pool of destinations makes the firm more likely to export occasionally to some markets, thereby raising volatility. These results cast doubt on the commonly held belief that diversification must decrease volatility.

A machine learning-based approach is proposed to predict the transient stability of power systems after a large disturbance. The post-disturbance trajectories of generator rotor angles are taken as a whole cluster, and 19 cluster features are defined to depict the overall transient stability characteristics of the power systems. A hybrid approach, which combines the linear support vector machine with the decision tree, is proposed to generate the final transient stability classifier. Comprehensive studies are conducted on the IEEE 39-bus and IEEE 145-bus test systems to verify the performance of the proposed approach. Test results show that by using the cluster features and the proposed approach, the transient stability of the power system can be predicted accurately with a shorter training time. Furthermore, the prediction classifier is robust to unknown load levels and network topologies, especially under situations when some generator measurements are unavailable and the number of input cluster...

A new finite element model is developed to perform vibration analysis of a cracked pipe. To formulate the method, the local flexibility coefficients of a part-through circumferential crack in a pipe that is subjected to axial force, shear force and bending moment are analytically derived using linear fracture mechanics. In particular, an adaptive Simpson method is utilized to carry out the numerical integration for calculating the flexibility coefficients. With the flexibility coefficients, a finite element model is established to study the vibration characteristics of the cracked pipe, with particular emphasis on the crack effect represented by change in natural frequency. As an illustrative application, the finite element model is utilized to identify a crack in a pipe by contour plots of frequency ratio as function of crack location and crack depth, with the crack location and depth identified accurately. The proposed method is effective in characterizing the vibration behavior of a pipe with a crack.

Recent models of international trade have identified product quality as an important determinant of bilateral trade flows. In this paper we examine the relationship between the characteristics of the export market and the aggregate quality of products using Chinese data. We find evidence that product unit values vary with standard gravity variables in a different manner across sectors of the Chinese economy and run contrary to earlier findings for the USA. These results are not compatible with existing heterogeneous firm trade models with constant mark-up and the extension to include product quality. We construct a heterogeneous firm trade model with quality differences and spatial price discrimination, and show that the model provides plausible explanations for our empirical finds as well as other existing findings in the literature.

With the rapid development of computer and information automatics, advanced manufacturing technologies (such as numerical control technology and computer-aided manufacturing) have been invented and matured. In the face of fierce competition in the international market of 21st century, high-tech application becomes one of the main battle fields. In recent years, reverse engineering technology has developed rapidly, through which the digital computer-aided design (CAD) models of products can be obtained rapidly and accurately; in association with optimization design, computer aided design and finite element analysis, reverse engineering technology significantly improves the development efficiency of new products. As one of the representative technologies of the third industrial revolution, three-dimensional (3D) printing technology is adopted in reverse engineering to realize rapid manufacturing of products in a lot of fields; in addition, 3D printing is gradually penetrating into each link of product design, attracting more and more attention from industrial and investment communities.

When trading across borders, firms choose between different payment contracts. Theoretically, this should allow firms to trade-off differences in financing costs and enforcement across countries. This paper provides evidence for this hypothesis employing firm-level data from a large number of developing countries. As predicted, international transactions are more likely paid after delivery when financing costs in the source country are high and when contract enforcement is low. We extend the theory and also show empirically that the more complex an industry is, the more important is contract enforcement and the less important are financing costs for the contract choice.

We previously reported that the highly reactive cell-free heme (CFH) is increased in the plasma of patients with chronic lung injury and causes pulmonary edema in animal model of acute respiratory distress syndrome (ARDS) post inhalation of halogen gas. However, the mechanisms by which CFH causes pulmonary edema are unclear. Herein we report for the first time that CFH and chlorinated lipids (formed by the interaction of halogen gas, Cl2, with plasmalogens) are increased in the plasma of patients exposed to Cl2 gas. Ex vivo incubation of red blood cells (RBC) with halogenated lipids caused oxidative damage to RBC cytoskeletal protein spectrin, resulting in hemolysis and release of CFH. Patch clamp and short circuit current measurements revealed that CFH inhibited the activity of amiloride-sensitive epithelial Na+ channel (ENaC) and cation sodium (Na+) channels in mouse alveolar cells and trans-epithelial Na+ transport across human airway cells with EC50 of 125 nM and 500 nM, respectively. Molecular modeling identified 22 putative heme-docking sites on ENaC (energy of binding range: 86–1563 kJ/mol) with at least 2 sites within its narrow transmembrane pore, potentially capable of blocking Na+ transport across the channel. A single intramuscular injection of the heme-scavenging protein, hemopexin (4 μg/kg body weight), one hour post halogen gas exposure, decreased plasma CFH and improved lung ENaC activity in mice. In conclusion, results suggested that CFH mediated inhibition of ENaC activity may be responsible for pulmonary edema post inhalation injury., Graphical abstract Image 1, Highlights • Red blood cells are prone to hemolysis in patients with lung injury. • Cell-free heme released from red blood cells is highly reactive. • Cell-free heme impairs the activity of amiloride-sensitive epithelial Na+ channel. • The impairment of epithelial Na+ channels causes pulmonary edema. • Removing cell-free heme improves the activity of Na+ channels in lung injury.

Combining the existing safety input data and characteristics, this paper uses the dynamic change method to solve the safety input problem. The origin, flow and characteristics of safety investment are analyzed, the skills of system dynamics modelling and the simulation modeling software Vensim are used to build the model, including models of safety precaution, safety control and accident risk transfer etc. Through simulation modeling, the configuration effects under different configuration schemes in an enterprise are completely decomposed. The results show that the effects of different configuration schemes on accident frequency, loss value and total accidents counts be obtained by applying the model built, which has great positive significance and practical value for dynamic safety investment.

Severe influenza (IAV) infection can develop into bronchopneumonia and edema, leading to acquired respiratory distress syndrome (ARDS) and pathophysiology. Underlying causes for pulmonary edema and aberrant fluid regulation largely remain unknown, particularly regarding the role of viral-mediated mechanisms. Herein, we show that distinct IAV strains reduced the functions of the epithelial sodium channel (ENaC) and the cystic fibrosis transmembrane regulator (CFTR) in murine respiratory and alveolar epithelia in vivo, as assessed by measurements of nasal potential differences and single-cell electrophysiology. Reduced ion channel activity was distinctly limited to virally infected cells in vivo and not bystander uninfected lung epithelium. Multiple lines of evidence indicated ENaC and CFTR dysfunction during the acute infection period; however, only CFTR dysfunction persisted beyond the infection period. ENaC, CFTR, and Na,K-ATPase activities and protein levels were also reduced in virally infected human airway epithelial cells. Reduced ENaC and CFTR led to changes in airway surface liquid morphology of human tracheobronchial cultures and airways of IAV-infected mice. Pharmacologic correction of CFTR function ameliorated IAV-induced physiologic changes. These changes are consistent with mucous stasis and pulmonary edema; furthermore, they indicate that repurposing therapeutic interventions correcting CFTR dysfunction may be efficacious for treatment of IAV lung pathophysiology.

Recent studies have indicated that Macrophage migration inhibitory factor (MIF) plays an important role in the prevention and treatment of asthma. However the role of MIF in airway inflammation and airway epithelial barrier disruption in house dust mite (HDM)-induced asthma has not been addressed. We hypothesized that MIF contributed to HDM-induced the production of Th2-associated cytokines and E-cadherin dysfunction in asthmatic mice and 16HBE cells. In vivo, a HDM-induced asthma mouse model was set up and mice treated with MIF antagonist ISO-1 after HDM. The mice treated with the ISO-1 ameliorated airway hyper-reactivity, airway inflammation, increased serum IgE levels, the aberrant arrangement of E-cadherin as well as the release of Th2 cytokines induced by HDM. In vitro, the exposure of 16HBE cells to HDM and rhMIF resulted in airway epithelial barrier disruption, inflammatory cytokine production and enhanced glycolytic flux. While these changes were attenuated by MIF siRNA treatment. Sequentially, treatment of 16HBE cells with PFKFB3 antagonist PFK15 significantly lowered rhMIF-induced these changes in 16HBE cells. Therefore, these results indicate that MIF may be an important contributor in airway inflammation and airway epithelial barrier disruption of HDM-induced asthma. Moreover, HDM specifically induces airway inflammation and airway epithelial barrier disruption of 16HBE cells through MIF-mediated enhancement of aerobic glycolysis.

This study aims to analyze the scientific research progress on human fatigue assessment (HFA) by using a bibliometric method, which was conducted a systematic analyses of 28,028 scientific papers about HFA from the Web of Science database, and the results were discussed from the following perspectives: temporal distribution, geographical distribution, discipline distribution, and key words distribution. In addition, this analysis also provides the network of author co-citation and key words. The results are summarized as follows: 1) the number of papers about HFA increased rapidly since 1990s; 2) The United States is a high-yield, high-cited country with 36.70% of the world's total literatures, and whose average citation index on HFA research is 47.89 per paper; China is the developing country with the largest number of publications, but its total citations and average citations are backward, only with an average of 21 citations per article; 3) The assessment of human fatigue shows the development trend of multidisciplinary intersecting; 4) There is a relative lack of cooperation among the world's leading scholars; the Dutch Gijs Bleijenberg is the most published scholar in the world, who have 108 articles on HFA research; the American scholar David Cella has the most influential article, with an h-index of 36; the distribution of Chinese scholars is relatively scattered, and no one has written more than five articles; 5) All of countries in the world had the same research topics, "muscle fatigue" is an important part of the HFA; China needs to pay more attention to the fatigue assessment associated with cancer. The results of the analysis can be used to enhance our understanding of HFA research and support further research in this area.

We present a heterogeneous-firm model in which management ability increases both production efficiency and product quality. Combining six micro-datasets on management practices, production and trade in Chinese and American firms, we find broad support for the model's predictions. First, better managed firms are more likely to export, sell more products to more destination countries, and earn higher export revenues and profits. Second, better managed exporters have higher prices, higher quality, and lower quality-adjusted prices. Finally, they also use a wider range of inputs, higher quality and more expensive inputs, and imported inputs from more advanced countries. The structural estimates indicate that management is important for improving production efficiency and product quality in both countries, but it matters more in China than in the US, especially for product quality. Panel analysis for the US and a randomized control trial in India suggest that management exerts causal effects on product quality, production efficiency, and exports. Poor management practices may thus hinder trade and growth, especially in developing countries.

Dangguiliuhuang decoction (DGLHD) has been demonstrated to be effective in treating inflammatory, hepatic steatosis, and insulin resistance. In the study, we tried to elucidate the pharmacological efficacy and mechanism of DGLHD against liver fibrosis and predicate potential active ingredients and targets via network analysis and experimental validation. In the formula, we totally discovered 76 potential active ingredients like baicalein, berberine, and wogonin, and 286 corresponding targets including PTGS (prostaglandin-endoperoxide synthase) 2, PPAR (peroxisome proliferator-activated receptors) -γ, and NF-κB (nuclear factor-κB). Pathway and functional enrichment analysis of these putative targets indicated that DGLHD obviously influenced NF-κB and PPAR signaling pathway. Consistently, DGLHD downregulated levels of ALT (alanine transaminase) and AST (aspartate transaminase), reduced production of proinflammatory cytokines-TNF (tumor necrosis factor) -α and IL (Interleukin) -1β in serum and liver from mice with hepatic fibrosis, and inhibited hepatic stellate cell (HSC)-T6 cells proliferation. DGLHD decreased TGF (transforming growth factor) -β1 and α-SMA (smooth muscle actin) expression as well, maintained MMP (matrix metalloprotein) 13-TIMP (tissue inhibitor of metalloproteinases) 1 balance, leading to mitigated ECM (extracellular matrix) deposition in vivo and in vitro. Moreover, our experimental data confirmed that the alleviated inflammation and ECM accumulation were pertinent to NF-κB inhibition and PPAR-γ activation. Overall, our results suggest that DGLHD aims at multiply targets and impedes the progression of hepatic fibrosis by ameliorating abnormal inflammation and ECM deposition, thereby serving as a novel regimen for treating hepatic fibrosis in clinic.

Acid (HCl) aspiration during anesthesia may lead to acute lung injury. There is no effective therapy. We hypothesized that HCl instilled intratracheally in C57BL/6 mice results in the formation of low-molecular weight hyaluronan (L-HA), which activates RhoA and Rho kinase (ROCK), causing airway hyperresponsiveness (AHR) and increased permeability. Furthermore, instillation of high-molecular weight hyaluronan (H-HA; Yabro) will reverse lung injury. We instilled HCl in C57BL/6 wild-type (WT), myeloperoxidase gene-deficient (MPO−/−) mice, and CD44 gene-deficient (CD44−/−) mice. WT mice were also instilled intranasally with H-HA (Yabro) at 1 and 23 h post-HCl. All measurements were performed at 1, 5, or 24 h post-HCl. Instillation of HCl in WT but not in CD44−/− resulted in increased inflammation, AHR, lung injury, and L-HA in the bronchoalveolar lavage fluid (BALF) 24 h post-HCl; L-HA levels and lung injury were significantly lower in HCl-instilled MPO−/− mice. Isolated perfused lungs of HCl instilled WT but not of CD44−/− mice had elevated values of the filtration coefficient ( Kf). Addition of L-HA on the apical surface of human primary bronchial epithelial cell monolayer decreased barrier resistance ( RT). H-HA significantly mitigated inflammation, AHR, and pulmonary vascular leakage at 24 h after HCl instillation and mitigated the increase of Kf and RT, as well as ROCK2 phosphorylation. Increased H- and L-HA levels were found in the BALF of mechanically ventilated patients but not in healthy volunteers. HCl instillation-induced lung injury is mediated by the L-HA-CD44-RhoA-ROCK2 signaling pathway, and H-HA is a potential novel therapeutic agent for acid aspiration-induced lung injury.

Chitin is a polysaccharide that provides structure and rigidity to the cell walls of fungi and insects. Mammals possess multiple chitinases, which function to degrade chitin, thereby supporting a role for chitinases in immune defense. However, chitin degradation has been implicated in the pathogenesis of asthma. Here, we determined the impact of acidic mammalian chitinase (AMCase) ( Chia ) deficiency on host defense during acute exposure to the fungal pathogen Aspergillus fumigatus as well as its contribution to A. fumigatus -associated allergic asthma. We demonstrate that chitin in the fungal cell wall was detected at low levels in A. fumigatus conidia, which emerged at the highest level during hyphal transition. In response to acute A. fumigatus challenge, Chia −/− mice unexpectedly demonstrated lower A. fumigatus lung burdens at 2 days postchallenge. The lower fungal burden correlated with decreased lung interleukin-33 (IL-33) levels yet increased IL-1β and prostaglandin E 2 (PGE 2 ) production, a phenotype that we reported previously to promote the induction of IL-17A and IL-22. During chronic A. fumigatus exposure, AMCase deficiency resulted in lower dynamic and airway lung resistance than in wild-type mice. Improved lung physiology correlated with attenuated levels of the proallergic chemokines CCL17 and CCL22. Surprisingly, examination of inflammatory responses during chronic exposure revealed attenuated IL-17A and IL-22 responses, but not type 2 responses, in the absence of AMCase. Collectively, these data suggest that AMCase functions as a negative regulator of immune responses during acute fungal exposure and is a contributor to fungal asthma severity, putatively via the induction of proinflammatory responses.

The arsenic adsorption capacity of a manganese oxide-modified biochar composite (MBC), prepared by pyrolysis of a mixture of potassium permanganate and biochar, was investigated in red soil. Adsorption experiments using batch procedures were used to estimate the arsenic adsorption capacities of the absorbent materials. Adsorption and desorption isotherms, Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) were used to characterise the prepared adsorbent materials, and a plausible mechanism for arsenic removal by MBC was proposed. Arsenic in red soil-MBC mixtures exhibited lower mobility than that in soils amended with pristine biochar. The improved removal performance of soil-MBC mixtures was attributed to a lower H/C ratio, higher O/C ratio, higher surface hydrophilicity, and higher surface sorption capacity, even though the impregnation of manganese oxide decreased the specific surface area of the biochar. Arsenic retention increased as the biochar content increased, mainly owing to an increase in soil pH. Several oxygenated functional groups, especially O-H, CO, Mn-O, and Si-O, participated in the adsorption process, and manganese oxides played a significant role in the oxidation of arsenic. This study highlights the potential of MBC as an absorbent to immobilise arsenic for use in contaminated land remediation in the red soils region.

Exposure to chlorine (Cl2) damages airway and alveolar epithelia resulting in acute lung injury and reactive airway hyperresponsiveness (AHR) to methacholine. However, little is known about the effect of preexisting respiratory disease on Cl2-induced lung injury. By using a murine respiratory syncytial virus (RSV) infection model, we found that preexisting RSV infection increases Cl2(187 ppm for 30 min)-induced lung inflammation and airway AHR at 24 h after exposure (5 days after infection). RSV infection and Cl2exposure synergistically induced oxygen desaturation and neutrophil infiltration and increased MCP-1, MIP-1β, IL-10, IFN-γ, and RANTES concentrations in the bronchoalveolar lavage fluid (BALF). In contrast, levels of type 2 cytokines (i.e., IL-4, IL-5, IL-9, and IL-13) were not significantly affected by either RSV infection or Cl2exposure. Cl2exposure, but not RSV infection, induced AHR to methacholine challenge as measured by flexiVent. Moreover, preexisting RSV infection amplified BALF levels of hyaluronan (HA) and AHR. The Cl2-induced AHR was mitigated by treatment with inter-α-trypsin inhibitor antibody, which inhibits HA signaling, suggesting a mechanism of HA-mediated AHR from exacerbated oxidative injury. Our results show for the first time that preexisting RSV infection predisposes the lung to Cl2-induced injury. These data emphasize the necessity for further research on the effects of Cl2in vulnerable populations and the development of appropriate treatments.

Pulmonary exposure to cadmium, a major component of cigarette smoke, has a dramatic impact on lung function and the development of emphysema. Cigarette smoke exposure induces heme oxygenase-1 (HO-1), a cytoprotective enzyme. In this study, we employed a truncated mouse model of emphysema by intratracheal instillation of cadmium (CdCl2) solution (0.025% per 1 mg/kg body wt) in HO-1+/+, HO-1−/−, and overexpressing humanized HO-1 bacterial artificial chromosome (hHO-1BAC) mice. We evaluated the role of HO-1 in cadmium-induced emphysema in mice by analyzing histopathology, micro-computed tomography scans, and lung function tests. CdCl2-exposed HO-1−/− mice exhibited more severe emphysema compared with HO-1+/+ or hHO-1BAC mice. Loss of pulmonary endothelial cells (PECs) from the alveolar capillary membrane is recognized to be a target in emphysema. PECs from HO-1+/+, HO-1−/−, and hHO-1BAC were employed to define the underlying molecular mechanism for the protection from emphysema by HO-1. Electron microscopy, expression of autophagic markers (microtubule-associated protein 1B-light chain 3 II, autophagy protein 5, and Beclin1) and apoptotic marker (cleaved caspase 3) suggested induction of autophagy and apoptosis in PECs after CdCl2 treatment. CdCl2-treated HO-1−/− PECs exhibited downregulation of autophagic markers and significantly increased cleaved caspase 3 expression and activity (∼4-fold higher). Moreover, hHO-1BAC PECs demonstrated upregulated autophagy and absence of cleaved caspase 3 expression or activity. Pretreatment of HO-1+/+ PECs with rapamycin induced autophagy and resulted in reduced cell death upon cadmium treatment. Induction of autophagy following CdCl2 treatment was found to be protective from apoptotic cell death. HO-1 induced protective autophagy in PECs and mitigated cadmium-induced emphysema.