101. [Screening for inhibitor of vascular endothelial growth factor from random peptide library].
- Author
-
Wu J, Zhang H, Wang J, Yang T, Xian J, Yang C, Zheng W, Chen H, and Wang Q
- Subjects
- Animals, Binding Sites, Endothelial Growth Factors metabolism, Enzyme-Linked Immunosorbent Assay, Humans, Intercellular Signaling Peptides and Proteins metabolism, Lymphokines metabolism, Peptides pharmacology, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors, Endothelial Growth Factors antagonists & inhibitors, Lymphokines antagonists & inhibitors, Peptide Library
- Abstract
Objective: To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library., Methods: Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT., Results: Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells., Conclusion: The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.
- Published
- 2002